Evaluation of DNA damage in COPD patients and its correlation with polymorphisms in repair genes
Background We investigated a potential link between genetic polymorphisms in genes XRCC1 (Arg399Gln), OGG1 (Ser326Cys), XRCC3 (Thr241Met), and XRCC4 (Ile401Thr) with the level of DNA damage and repair, accessed by comet and micronucleus test, in 51 COPD patients and 51 controls. Methods Peripheral b...
Ausführliche Beschreibung
Autor*in: |
da Silva, Andréa Lúcia Gonçalves [verfasserIn] |
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E-Artikel |
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Englisch |
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2013 |
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Anmerkung: |
© da Silva et al.; licensee BioMed Central Ltd. 2013 |
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Übergeordnetes Werk: |
Enthalten in: BMC medical genetics - London : BioMed Central, 2000, 14(2013), 1 vom: 20. Sept. |
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Übergeordnetes Werk: |
volume:14 ; year:2013 ; number:1 ; day:20 ; month:09 |
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DOI / URN: |
10.1186/1471-2350-14-93 |
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Katalog-ID: |
SPR027492052 |
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100 | 1 | |a da Silva, Andréa Lúcia Gonçalves |e verfasserin |4 aut | |
245 | 1 | 0 | |a Evaluation of DNA damage in COPD patients and its correlation with polymorphisms in repair genes |
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520 | |a Background We investigated a potential link between genetic polymorphisms in genes XRCC1 (Arg399Gln), OGG1 (Ser326Cys), XRCC3 (Thr241Met), and XRCC4 (Ile401Thr) with the level of DNA damage and repair, accessed by comet and micronucleus test, in 51 COPD patients and 51 controls. Methods Peripheral blood was used to perform the alkaline and neutral comet assay; and genetic polymorphisms by PCR/RFLP. To assess the susceptibility to exogenous DNA damage, the cells were treated with methyl methanesulphonate for 1-h or 3-h. After 3-h treatment the % residual damage was calculated assuming the value of 1-h treatment as 100%. The cytogenetic damage was evaluated by buccal micronucleus cytome assay (BMCyt). Results COPD patients with the risk allele XRCC1 (Arg399Gln) and XRCC3 (Thr241Met) showed higher DNA damage by comet assay. The residual damage was higher for COPD with risk allele in the four genes. In COPD patients was showed negative correlation between BMCyt (binucleated, nuclear bud, condensed chromatin and karyorrhexic cells) with pulmonary function and some variant genotypes. Conclusion Our results suggest a possible association between variant genotypes in XRCC1 (Arg399Gln), OGG1 (Ser326Cys), XRCC3 (Thr241Met), and XRCC4 (Ile401Thr), DNA damage and progression of COPD. | ||
650 | 4 | |a COPD |7 (dpeaa)DE-He213 | |
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650 | 4 | |a Genetic polymorphisms |7 (dpeaa)DE-He213 | |
650 | 4 | |a BMCyt |7 (dpeaa)DE-He213 | |
700 | 1 | |a da Rosa, Helen Tais |4 aut | |
700 | 1 | |a Karnopp, Thaís Evelyn |4 aut | |
700 | 1 | |a Charlier, Clara Forrer |4 aut | |
700 | 1 | |a Ellwanger, Joel Henrique |4 aut | |
700 | 1 | |a Moura, Dinara Jaqueline |4 aut | |
700 | 1 | |a Possuelo, Lia Gonçalves |4 aut | |
700 | 1 | |a Valim, Andréia Rosane de Moura |4 aut | |
700 | 1 | |a Guecheva, Temenouga Nikolova |4 aut | |
700 | 1 | |a Henriques, João Antonio Pêgas |4 aut | |
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10.1186/1471-2350-14-93 doi (DE-627)SPR027492052 (SPR)1471-2350-14-93-e DE-627 ger DE-627 rakwb eng da Silva, Andréa Lúcia Gonçalves verfasserin aut Evaluation of DNA damage in COPD patients and its correlation with polymorphisms in repair genes 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © da Silva et al.; licensee BioMed Central Ltd. 2013 Background We investigated a potential link between genetic polymorphisms in genes XRCC1 (Arg399Gln), OGG1 (Ser326Cys), XRCC3 (Thr241Met), and XRCC4 (Ile401Thr) with the level of DNA damage and repair, accessed by comet and micronucleus test, in 51 COPD patients and 51 controls. Methods Peripheral blood was used to perform the alkaline and neutral comet assay; and genetic polymorphisms by PCR/RFLP. To assess the susceptibility to exogenous DNA damage, the cells were treated with methyl methanesulphonate for 1-h or 3-h. After 3-h treatment the % residual damage was calculated assuming the value of 1-h treatment as 100%. The cytogenetic damage was evaluated by buccal micronucleus cytome assay (BMCyt). Results COPD patients with the risk allele XRCC1 (Arg399Gln) and XRCC3 (Thr241Met) showed higher DNA damage by comet assay. The residual damage was higher for COPD with risk allele in the four genes. In COPD patients was showed negative correlation between BMCyt (binucleated, nuclear bud, condensed chromatin and karyorrhexic cells) with pulmonary function and some variant genotypes. Conclusion Our results suggest a possible association between variant genotypes in XRCC1 (Arg399Gln), OGG1 (Ser326Cys), XRCC3 (Thr241Met), and XRCC4 (Ile401Thr), DNA damage and progression of COPD. COPD (dpeaa)DE-He213 DNA damage (dpeaa)DE-He213 DNA repair (dpeaa)DE-He213 Genetic polymorphisms (dpeaa)DE-He213 BMCyt (dpeaa)DE-He213 da Rosa, Helen Tais aut Karnopp, Thaís Evelyn aut Charlier, Clara Forrer aut Ellwanger, Joel Henrique aut Moura, Dinara Jaqueline aut Possuelo, Lia Gonçalves aut Valim, Andréia Rosane de Moura aut Guecheva, Temenouga Nikolova aut Henriques, João Antonio Pêgas aut Enthalten in BMC medical genetics London : BioMed Central, 2000 14(2013), 1 vom: 20. Sept. (DE-627)326643788 (DE-600)2041359-2 1471-2350 nnns volume:14 year:2013 number:1 day:20 month:09 https://dx.doi.org/10.1186/1471-2350-14-93 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2013 1 20 09 |
spelling |
10.1186/1471-2350-14-93 doi (DE-627)SPR027492052 (SPR)1471-2350-14-93-e DE-627 ger DE-627 rakwb eng da Silva, Andréa Lúcia Gonçalves verfasserin aut Evaluation of DNA damage in COPD patients and its correlation with polymorphisms in repair genes 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © da Silva et al.; licensee BioMed Central Ltd. 2013 Background We investigated a potential link between genetic polymorphisms in genes XRCC1 (Arg399Gln), OGG1 (Ser326Cys), XRCC3 (Thr241Met), and XRCC4 (Ile401Thr) with the level of DNA damage and repair, accessed by comet and micronucleus test, in 51 COPD patients and 51 controls. Methods Peripheral blood was used to perform the alkaline and neutral comet assay; and genetic polymorphisms by PCR/RFLP. To assess the susceptibility to exogenous DNA damage, the cells were treated with methyl methanesulphonate for 1-h or 3-h. After 3-h treatment the % residual damage was calculated assuming the value of 1-h treatment as 100%. The cytogenetic damage was evaluated by buccal micronucleus cytome assay (BMCyt). Results COPD patients with the risk allele XRCC1 (Arg399Gln) and XRCC3 (Thr241Met) showed higher DNA damage by comet assay. The residual damage was higher for COPD with risk allele in the four genes. In COPD patients was showed negative correlation between BMCyt (binucleated, nuclear bud, condensed chromatin and karyorrhexic cells) with pulmonary function and some variant genotypes. Conclusion Our results suggest a possible association between variant genotypes in XRCC1 (Arg399Gln), OGG1 (Ser326Cys), XRCC3 (Thr241Met), and XRCC4 (Ile401Thr), DNA damage and progression of COPD. COPD (dpeaa)DE-He213 DNA damage (dpeaa)DE-He213 DNA repair (dpeaa)DE-He213 Genetic polymorphisms (dpeaa)DE-He213 BMCyt (dpeaa)DE-He213 da Rosa, Helen Tais aut Karnopp, Thaís Evelyn aut Charlier, Clara Forrer aut Ellwanger, Joel Henrique aut Moura, Dinara Jaqueline aut Possuelo, Lia Gonçalves aut Valim, Andréia Rosane de Moura aut Guecheva, Temenouga Nikolova aut Henriques, João Antonio Pêgas aut Enthalten in BMC medical genetics London : BioMed Central, 2000 14(2013), 1 vom: 20. Sept. (DE-627)326643788 (DE-600)2041359-2 1471-2350 nnns volume:14 year:2013 number:1 day:20 month:09 https://dx.doi.org/10.1186/1471-2350-14-93 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2013 1 20 09 |
allfields_unstemmed |
10.1186/1471-2350-14-93 doi (DE-627)SPR027492052 (SPR)1471-2350-14-93-e DE-627 ger DE-627 rakwb eng da Silva, Andréa Lúcia Gonçalves verfasserin aut Evaluation of DNA damage in COPD patients and its correlation with polymorphisms in repair genes 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © da Silva et al.; licensee BioMed Central Ltd. 2013 Background We investigated a potential link between genetic polymorphisms in genes XRCC1 (Arg399Gln), OGG1 (Ser326Cys), XRCC3 (Thr241Met), and XRCC4 (Ile401Thr) with the level of DNA damage and repair, accessed by comet and micronucleus test, in 51 COPD patients and 51 controls. Methods Peripheral blood was used to perform the alkaline and neutral comet assay; and genetic polymorphisms by PCR/RFLP. To assess the susceptibility to exogenous DNA damage, the cells were treated with methyl methanesulphonate for 1-h or 3-h. After 3-h treatment the % residual damage was calculated assuming the value of 1-h treatment as 100%. The cytogenetic damage was evaluated by buccal micronucleus cytome assay (BMCyt). Results COPD patients with the risk allele XRCC1 (Arg399Gln) and XRCC3 (Thr241Met) showed higher DNA damage by comet assay. The residual damage was higher for COPD with risk allele in the four genes. In COPD patients was showed negative correlation between BMCyt (binucleated, nuclear bud, condensed chromatin and karyorrhexic cells) with pulmonary function and some variant genotypes. Conclusion Our results suggest a possible association between variant genotypes in XRCC1 (Arg399Gln), OGG1 (Ser326Cys), XRCC3 (Thr241Met), and XRCC4 (Ile401Thr), DNA damage and progression of COPD. COPD (dpeaa)DE-He213 DNA damage (dpeaa)DE-He213 DNA repair (dpeaa)DE-He213 Genetic polymorphisms (dpeaa)DE-He213 BMCyt (dpeaa)DE-He213 da Rosa, Helen Tais aut Karnopp, Thaís Evelyn aut Charlier, Clara Forrer aut Ellwanger, Joel Henrique aut Moura, Dinara Jaqueline aut Possuelo, Lia Gonçalves aut Valim, Andréia Rosane de Moura aut Guecheva, Temenouga Nikolova aut Henriques, João Antonio Pêgas aut Enthalten in BMC medical genetics London : BioMed Central, 2000 14(2013), 1 vom: 20. Sept. (DE-627)326643788 (DE-600)2041359-2 1471-2350 nnns volume:14 year:2013 number:1 day:20 month:09 https://dx.doi.org/10.1186/1471-2350-14-93 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2013 1 20 09 |
allfieldsGer |
10.1186/1471-2350-14-93 doi (DE-627)SPR027492052 (SPR)1471-2350-14-93-e DE-627 ger DE-627 rakwb eng da Silva, Andréa Lúcia Gonçalves verfasserin aut Evaluation of DNA damage in COPD patients and its correlation with polymorphisms in repair genes 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © da Silva et al.; licensee BioMed Central Ltd. 2013 Background We investigated a potential link between genetic polymorphisms in genes XRCC1 (Arg399Gln), OGG1 (Ser326Cys), XRCC3 (Thr241Met), and XRCC4 (Ile401Thr) with the level of DNA damage and repair, accessed by comet and micronucleus test, in 51 COPD patients and 51 controls. Methods Peripheral blood was used to perform the alkaline and neutral comet assay; and genetic polymorphisms by PCR/RFLP. To assess the susceptibility to exogenous DNA damage, the cells were treated with methyl methanesulphonate for 1-h or 3-h. After 3-h treatment the % residual damage was calculated assuming the value of 1-h treatment as 100%. The cytogenetic damage was evaluated by buccal micronucleus cytome assay (BMCyt). Results COPD patients with the risk allele XRCC1 (Arg399Gln) and XRCC3 (Thr241Met) showed higher DNA damage by comet assay. The residual damage was higher for COPD with risk allele in the four genes. In COPD patients was showed negative correlation between BMCyt (binucleated, nuclear bud, condensed chromatin and karyorrhexic cells) with pulmonary function and some variant genotypes. Conclusion Our results suggest a possible association between variant genotypes in XRCC1 (Arg399Gln), OGG1 (Ser326Cys), XRCC3 (Thr241Met), and XRCC4 (Ile401Thr), DNA damage and progression of COPD. COPD (dpeaa)DE-He213 DNA damage (dpeaa)DE-He213 DNA repair (dpeaa)DE-He213 Genetic polymorphisms (dpeaa)DE-He213 BMCyt (dpeaa)DE-He213 da Rosa, Helen Tais aut Karnopp, Thaís Evelyn aut Charlier, Clara Forrer aut Ellwanger, Joel Henrique aut Moura, Dinara Jaqueline aut Possuelo, Lia Gonçalves aut Valim, Andréia Rosane de Moura aut Guecheva, Temenouga Nikolova aut Henriques, João Antonio Pêgas aut Enthalten in BMC medical genetics London : BioMed Central, 2000 14(2013), 1 vom: 20. Sept. (DE-627)326643788 (DE-600)2041359-2 1471-2350 nnns volume:14 year:2013 number:1 day:20 month:09 https://dx.doi.org/10.1186/1471-2350-14-93 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2013 1 20 09 |
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10.1186/1471-2350-14-93 doi (DE-627)SPR027492052 (SPR)1471-2350-14-93-e DE-627 ger DE-627 rakwb eng da Silva, Andréa Lúcia Gonçalves verfasserin aut Evaluation of DNA damage in COPD patients and its correlation with polymorphisms in repair genes 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © da Silva et al.; licensee BioMed Central Ltd. 2013 Background We investigated a potential link between genetic polymorphisms in genes XRCC1 (Arg399Gln), OGG1 (Ser326Cys), XRCC3 (Thr241Met), and XRCC4 (Ile401Thr) with the level of DNA damage and repair, accessed by comet and micronucleus test, in 51 COPD patients and 51 controls. Methods Peripheral blood was used to perform the alkaline and neutral comet assay; and genetic polymorphisms by PCR/RFLP. To assess the susceptibility to exogenous DNA damage, the cells were treated with methyl methanesulphonate for 1-h or 3-h. After 3-h treatment the % residual damage was calculated assuming the value of 1-h treatment as 100%. The cytogenetic damage was evaluated by buccal micronucleus cytome assay (BMCyt). Results COPD patients with the risk allele XRCC1 (Arg399Gln) and XRCC3 (Thr241Met) showed higher DNA damage by comet assay. The residual damage was higher for COPD with risk allele in the four genes. In COPD patients was showed negative correlation between BMCyt (binucleated, nuclear bud, condensed chromatin and karyorrhexic cells) with pulmonary function and some variant genotypes. Conclusion Our results suggest a possible association between variant genotypes in XRCC1 (Arg399Gln), OGG1 (Ser326Cys), XRCC3 (Thr241Met), and XRCC4 (Ile401Thr), DNA damage and progression of COPD. COPD (dpeaa)DE-He213 DNA damage (dpeaa)DE-He213 DNA repair (dpeaa)DE-He213 Genetic polymorphisms (dpeaa)DE-He213 BMCyt (dpeaa)DE-He213 da Rosa, Helen Tais aut Karnopp, Thaís Evelyn aut Charlier, Clara Forrer aut Ellwanger, Joel Henrique aut Moura, Dinara Jaqueline aut Possuelo, Lia Gonçalves aut Valim, Andréia Rosane de Moura aut Guecheva, Temenouga Nikolova aut Henriques, João Antonio Pêgas aut Enthalten in BMC medical genetics London : BioMed Central, 2000 14(2013), 1 vom: 20. Sept. (DE-627)326643788 (DE-600)2041359-2 1471-2350 nnns volume:14 year:2013 number:1 day:20 month:09 https://dx.doi.org/10.1186/1471-2350-14-93 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2013 1 20 09 |
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da Silva, Andréa Lúcia Gonçalves @@aut@@ da Rosa, Helen Tais @@aut@@ Karnopp, Thaís Evelyn @@aut@@ Charlier, Clara Forrer @@aut@@ Ellwanger, Joel Henrique @@aut@@ Moura, Dinara Jaqueline @@aut@@ Possuelo, Lia Gonçalves @@aut@@ Valim, Andréia Rosane de Moura @@aut@@ Guecheva, Temenouga Nikolova @@aut@@ Henriques, João Antonio Pêgas @@aut@@ |
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Evaluation of DNA damage in COPD patients and its correlation with polymorphisms in repair genes |
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da Silva, Andréa Lúcia Gonçalves |
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BMC medical genetics |
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da Silva, Andréa Lúcia Gonçalves da Rosa, Helen Tais Karnopp, Thaís Evelyn Charlier, Clara Forrer Ellwanger, Joel Henrique Moura, Dinara Jaqueline Possuelo, Lia Gonçalves Valim, Andréia Rosane de Moura Guecheva, Temenouga Nikolova Henriques, João Antonio Pêgas |
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da Silva, Andréa Lúcia Gonçalves |
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10.1186/1471-2350-14-93 |
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evaluation of dna damage in copd patients and its correlation with polymorphisms in repair genes |
title_auth |
Evaluation of DNA damage in COPD patients and its correlation with polymorphisms in repair genes |
abstract |
Background We investigated a potential link between genetic polymorphisms in genes XRCC1 (Arg399Gln), OGG1 (Ser326Cys), XRCC3 (Thr241Met), and XRCC4 (Ile401Thr) with the level of DNA damage and repair, accessed by comet and micronucleus test, in 51 COPD patients and 51 controls. Methods Peripheral blood was used to perform the alkaline and neutral comet assay; and genetic polymorphisms by PCR/RFLP. To assess the susceptibility to exogenous DNA damage, the cells were treated with methyl methanesulphonate for 1-h or 3-h. After 3-h treatment the % residual damage was calculated assuming the value of 1-h treatment as 100%. The cytogenetic damage was evaluated by buccal micronucleus cytome assay (BMCyt). Results COPD patients with the risk allele XRCC1 (Arg399Gln) and XRCC3 (Thr241Met) showed higher DNA damage by comet assay. The residual damage was higher for COPD with risk allele in the four genes. In COPD patients was showed negative correlation between BMCyt (binucleated, nuclear bud, condensed chromatin and karyorrhexic cells) with pulmonary function and some variant genotypes. Conclusion Our results suggest a possible association between variant genotypes in XRCC1 (Arg399Gln), OGG1 (Ser326Cys), XRCC3 (Thr241Met), and XRCC4 (Ile401Thr), DNA damage and progression of COPD. © da Silva et al.; licensee BioMed Central Ltd. 2013 |
abstractGer |
Background We investigated a potential link between genetic polymorphisms in genes XRCC1 (Arg399Gln), OGG1 (Ser326Cys), XRCC3 (Thr241Met), and XRCC4 (Ile401Thr) with the level of DNA damage and repair, accessed by comet and micronucleus test, in 51 COPD patients and 51 controls. Methods Peripheral blood was used to perform the alkaline and neutral comet assay; and genetic polymorphisms by PCR/RFLP. To assess the susceptibility to exogenous DNA damage, the cells were treated with methyl methanesulphonate for 1-h or 3-h. After 3-h treatment the % residual damage was calculated assuming the value of 1-h treatment as 100%. The cytogenetic damage was evaluated by buccal micronucleus cytome assay (BMCyt). Results COPD patients with the risk allele XRCC1 (Arg399Gln) and XRCC3 (Thr241Met) showed higher DNA damage by comet assay. The residual damage was higher for COPD with risk allele in the four genes. In COPD patients was showed negative correlation between BMCyt (binucleated, nuclear bud, condensed chromatin and karyorrhexic cells) with pulmonary function and some variant genotypes. Conclusion Our results suggest a possible association between variant genotypes in XRCC1 (Arg399Gln), OGG1 (Ser326Cys), XRCC3 (Thr241Met), and XRCC4 (Ile401Thr), DNA damage and progression of COPD. © da Silva et al.; licensee BioMed Central Ltd. 2013 |
abstract_unstemmed |
Background We investigated a potential link between genetic polymorphisms in genes XRCC1 (Arg399Gln), OGG1 (Ser326Cys), XRCC3 (Thr241Met), and XRCC4 (Ile401Thr) with the level of DNA damage and repair, accessed by comet and micronucleus test, in 51 COPD patients and 51 controls. Methods Peripheral blood was used to perform the alkaline and neutral comet assay; and genetic polymorphisms by PCR/RFLP. To assess the susceptibility to exogenous DNA damage, the cells were treated with methyl methanesulphonate for 1-h or 3-h. After 3-h treatment the % residual damage was calculated assuming the value of 1-h treatment as 100%. The cytogenetic damage was evaluated by buccal micronucleus cytome assay (BMCyt). Results COPD patients with the risk allele XRCC1 (Arg399Gln) and XRCC3 (Thr241Met) showed higher DNA damage by comet assay. The residual damage was higher for COPD with risk allele in the four genes. In COPD patients was showed negative correlation between BMCyt (binucleated, nuclear bud, condensed chromatin and karyorrhexic cells) with pulmonary function and some variant genotypes. Conclusion Our results suggest a possible association between variant genotypes in XRCC1 (Arg399Gln), OGG1 (Ser326Cys), XRCC3 (Thr241Met), and XRCC4 (Ile401Thr), DNA damage and progression of COPD. © da Silva et al.; licensee BioMed Central Ltd. 2013 |
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Evaluation of DNA damage in COPD patients and its correlation with polymorphisms in repair genes |
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da Rosa, Helen Tais Karnopp, Thaís Evelyn Charlier, Clara Forrer Ellwanger, Joel Henrique Moura, Dinara Jaqueline Possuelo, Lia Gonçalves Valim, Andréia Rosane de Moura Guecheva, Temenouga Nikolova Henriques, João Antonio Pêgas |
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