Alteration of protein expression pattern of vascular endothelial growth factor (VEGF) from soluble to cell-associated isoform during tumourigenesis

Background Vascular endothelial growth factor (VEGF) is a potent mitogen for endothelial cells, and its expression has been correlated with increased tumour angiogenesis. Although numerous publications dealing with the measurement of circulating VEGF for diagnostic and therapeutic monitoring have be...
Ausführliche Beschreibung

Background Vascular endothelial growth factor (VEGF) is a potent mitogen for endothelial cells, and its expression has been correlated with increased tumour angiogenesis. Although numerous publications dealing with the measurement of circulating VEGF for diagnostic and therapeutic monitoring have been published, the relationship between the production of tissue VEGF and its concentration in blood is still unclear. The aims of this study were to determine: 1) The expression pattern of VEGF isoforms at the protein level in colorectal and lung adenocarcinoma in comparison to the pattern in corresponding adjacent normal tissues 2) The relationship between the expression pattern of VEGF and total level of circulating VEGF in the blood to clarify whether the results of measuring circulating VEGF can be used to predict VEGF expression in tumour tissues. Methods Ninety-four tissue samples were obtained from patients, 76 colorectal tumour tissues and 18 lung tumour tissues. VEGF protein expression pattern and total circulating VEGF were examined using western blot and capture ELISA, respectively. Results Three major protein bands were predominately detected in tumour samples with an apparent molecular mass under reducing conditions of 18, 23 and 26 kDa. The 18 kDa VEGF protein was expressed equally in both normal and colorectal tumour tissues and predominately expressed in normal tissues of lung, whereas the 23 and 26 kDa protein was only detected at higher levels in tumour tissues. The 18, 23 and 26 kDa proteins are believed to represent the $ VEGF_{121} $, the $ VEGF_{165} $ and the $ VEGF_{189} $, respectively. There was a significant correlation of the expression of $ VEGF_{165} $ with a smaller tumour size maximum diameter <5 cm (p < 0.05), and there was a significant correlation of $ VEGF_{189} $ with advanced clinical stage of colorectal tumours. The measurement of total circulating VEGF in serum revealed that cancer patients significantly (p < 0.001) possessed a higher level of circulating VEGF (1081 ± 652 pg/ml in colorectal and 1,251 ± 568 pg/ml in lung) than a healthy volunteer group (543 ± 344 pg/ml). No correlation between the level of circulating VEGF and the pathologic features of tumours was observed. Conclusion Our findings indicate that the expression patterns of VEGF isoforms are altered during tumourigenesis as certain isoform overexpression in tumour tissues correlated with tumour progression indicating their important role in tumour development. However, measurement of VEGF in the circulation as a prognostic marker needs to be carefully evaluated as the cell-associated isoform ($ VEGF_{189} $), but not the soluble isoform ($ VEGF_{121} $ and $ VEGF_{165} $) appears to play important role in tumour progression. Ausführliche Beschreibung

Autor*in:	Cressey, Ratchada [verfasserIn] Wattananupong, Onusa Lertprasertsuke, Nirush Vinitketkumnuen, Usanee

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2005

Schlagwörter:	Vascular Endothelial Growth Factor Vascular Endothelial Growth Factor Expression Vascular Endothelial Growth Factor Level Vascular Endothelial Growth Factor Protein Advanced Clinical Stage

Anmerkung:	© Cressey et al; licensee BioMed Central Ltd. 2005. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (

Übergeordnetes Werk:	Enthalten in: BMC cancer - London : BioMed Central, 2001, 5(2005), 1 vom: 04. Okt.
Übergeordnetes Werk:	volume:5 ; year:2005 ; number:1 ; day:04 ; month:10

Links:	Volltext

DOI / URN:	10.1186/1471-2407-5-128

Katalog-ID:	SPR027606163