Microarray-based identification and RT-PCR test screening for epithelial-specific mRNAs in peripheral blood of patients with colon cancer

Background The efficacy of screening for colorectal cancer using a simple blood-based assay for the detection of tumor cells disseminated in the circulation at an early stage of the disease is gaining positive feedback from several lines of research. This method seems able to reduce colorectal cance...
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Autor*in:	Solmi, Rossella [verfasserIn] Ugolini, Giampaolo Rosati, Giancarlo Zanotti, Simone Lauriola, Mattia Montroni, Isacco del Governatore, Marco Caira, Antonello Taffurelli, Mario Santini, Donatella Coppola, Domenico Guidotti, Lia Carinci, Paolo Strippoli, Pierluigi

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2006

Schlagwörter:	Circulate Tumor Cell mRNA Marker Normal Human Colon KRT20 mRNA KRT20 mRNA Expression

Anmerkung:	© Solmi et al; licensee BioMed Central Ltd. 2006

Übergeordnetes Werk:	Enthalten in: BMC cancer - London : BioMed Central, 2001, 6(2006), 1 vom: 20. Okt.
Übergeordnetes Werk:	volume:6 ; year:2006 ; number:1 ; day:20 ; month:10

Links:	Volltext

DOI / URN:	10.1186/1471-2407-6-250

Katalog-ID:	SPR027609340

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245	1	0	\|a Microarray-based identification and RT-PCR test screening for epithelial-specific mRNAs in peripheral blood of patients with colon cancer
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520			\|a Background The efficacy of screening for colorectal cancer using a simple blood-based assay for the detection of tumor cells disseminated in the circulation at an early stage of the disease is gaining positive feedback from several lines of research. This method seems able to reduce colorectal cancer mortality and may replace colonoscopy as the most effective means of detecting colonic lesions. Methods In this work, we present a new microarray-based high-throughput screening method to identifying candidate marker mRNAs for the early detection of epithelial cells diluted in peripheral blood cells. This method includes 1. direct comparison of different samples of colonic mucosa and of blood cells to identify consistent epithelial-specific mRNAs from among 20,000 cDNA assayed by microarray slides; 2. identification of candidate marker mRNAs by data analysis, which allowed selection of only 10 putative differentially expressed genes; 3. Selection of some of the most suitable mRNAs (TMEM69, RANBP3 and PRSS22) that were assayed in blood samples from normal subjects and patients with colon cancer as possible markers for the presence of epithelial cells in the blood, using reverse transcription – polymerase chain reaction (RT-PCR). Results Our present results seem to provide an indication, for the first time obtained by genome-scale screening, that a suitable and consistent colon epithelium mRNA marker may be difficult to identify. Conclusion The design of new approaches to identify such markers is warranted.
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700	1		\|a Rosati, Giancarlo \|4 aut
700	1		\|a Zanotti, Simone \|4 aut
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700	1		\|a Montroni, Isacco \|4 aut
700	1		\|a del Governatore, Marco \|4 aut
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700	1		\|a Taffurelli, Mario \|4 aut
700	1		\|a Santini, Donatella \|4 aut
700	1		\|a Coppola, Domenico \|4 aut
700	1		\|a Guidotti, Lia \|4 aut
700	1		\|a Carinci, Paolo \|4 aut
700	1		\|a Strippoli, Pierluigi \|4 aut
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publishDate	2006
allfields	10.1186/1471-2407-6-250 doi (DE-627)SPR027609340 (SPR)1471-2407-6-250-e DE-627 ger DE-627 rakwb eng Solmi, Rossella verfasserin aut Microarray-based identification and RT-PCR test screening for epithelial-specific mRNAs in peripheral blood of patients with colon cancer 2006 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Solmi et al; licensee BioMed Central Ltd. 2006 Background The efficacy of screening for colorectal cancer using a simple blood-based assay for the detection of tumor cells disseminated in the circulation at an early stage of the disease is gaining positive feedback from several lines of research. This method seems able to reduce colorectal cancer mortality and may replace colonoscopy as the most effective means of detecting colonic lesions. Methods In this work, we present a new microarray-based high-throughput screening method to identifying candidate marker mRNAs for the early detection of epithelial cells diluted in peripheral blood cells. This method includes 1. direct comparison of different samples of colonic mucosa and of blood cells to identify consistent epithelial-specific mRNAs from among 20,000 cDNA assayed by microarray slides; 2. identification of candidate marker mRNAs by data analysis, which allowed selection of only 10 putative differentially expressed genes; 3. Selection of some of the most suitable mRNAs (TMEM69, RANBP3 and PRSS22) that were assayed in blood samples from normal subjects and patients with colon cancer as possible markers for the presence of epithelial cells in the blood, using reverse transcription – polymerase chain reaction (RT-PCR). Results Our present results seem to provide an indication, for the first time obtained by genome-scale screening, that a suitable and consistent colon epithelium mRNA marker may be difficult to identify. Conclusion The design of new approaches to identify such markers is warranted. Circulate Tumor Cell (dpeaa)DE-He213 mRNA Marker (dpeaa)DE-He213 Normal Human Colon (dpeaa)DE-He213 KRT20 mRNA (dpeaa)DE-He213 KRT20 mRNA Expression (dpeaa)DE-He213 Ugolini, Giampaolo aut Rosati, Giancarlo aut Zanotti, Simone aut Lauriola, Mattia aut Montroni, Isacco aut del Governatore, Marco aut Caira, Antonello aut Taffurelli, Mario aut Santini, Donatella aut Coppola, Domenico aut Guidotti, Lia aut Carinci, Paolo aut Strippoli, Pierluigi aut Enthalten in BMC cancer London : BioMed Central, 2001 6(2006), 1 vom: 20. Okt. (DE-627)326643710 (DE-600)2041352-X 1471-2407 nnns volume:6 year:2006 number:1 day:20 month:10 https://dx.doi.org/10.1186/1471-2407-6-250 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2006 1 20 10
spelling	10.1186/1471-2407-6-250 doi (DE-627)SPR027609340 (SPR)1471-2407-6-250-e DE-627 ger DE-627 rakwb eng Solmi, Rossella verfasserin aut Microarray-based identification and RT-PCR test screening for epithelial-specific mRNAs in peripheral blood of patients with colon cancer 2006 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Solmi et al; licensee BioMed Central Ltd. 2006 Background The efficacy of screening for colorectal cancer using a simple blood-based assay for the detection of tumor cells disseminated in the circulation at an early stage of the disease is gaining positive feedback from several lines of research. This method seems able to reduce colorectal cancer mortality and may replace colonoscopy as the most effective means of detecting colonic lesions. Methods In this work, we present a new microarray-based high-throughput screening method to identifying candidate marker mRNAs for the early detection of epithelial cells diluted in peripheral blood cells. This method includes 1. direct comparison of different samples of colonic mucosa and of blood cells to identify consistent epithelial-specific mRNAs from among 20,000 cDNA assayed by microarray slides; 2. identification of candidate marker mRNAs by data analysis, which allowed selection of only 10 putative differentially expressed genes; 3. Selection of some of the most suitable mRNAs (TMEM69, RANBP3 and PRSS22) that were assayed in blood samples from normal subjects and patients with colon cancer as possible markers for the presence of epithelial cells in the blood, using reverse transcription – polymerase chain reaction (RT-PCR). Results Our present results seem to provide an indication, for the first time obtained by genome-scale screening, that a suitable and consistent colon epithelium mRNA marker may be difficult to identify. Conclusion The design of new approaches to identify such markers is warranted. Circulate Tumor Cell (dpeaa)DE-He213 mRNA Marker (dpeaa)DE-He213 Normal Human Colon (dpeaa)DE-He213 KRT20 mRNA (dpeaa)DE-He213 KRT20 mRNA Expression (dpeaa)DE-He213 Ugolini, Giampaolo aut Rosati, Giancarlo aut Zanotti, Simone aut Lauriola, Mattia aut Montroni, Isacco aut del Governatore, Marco aut Caira, Antonello aut Taffurelli, Mario aut Santini, Donatella aut Coppola, Domenico aut Guidotti, Lia aut Carinci, Paolo aut Strippoli, Pierluigi aut Enthalten in BMC cancer London : BioMed Central, 2001 6(2006), 1 vom: 20. Okt. (DE-627)326643710 (DE-600)2041352-X 1471-2407 nnns volume:6 year:2006 number:1 day:20 month:10 https://dx.doi.org/10.1186/1471-2407-6-250 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2006 1 20 10
allfields_unstemmed	10.1186/1471-2407-6-250 doi (DE-627)SPR027609340 (SPR)1471-2407-6-250-e DE-627 ger DE-627 rakwb eng Solmi, Rossella verfasserin aut Microarray-based identification and RT-PCR test screening for epithelial-specific mRNAs in peripheral blood of patients with colon cancer 2006 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Solmi et al; licensee BioMed Central Ltd. 2006 Background The efficacy of screening for colorectal cancer using a simple blood-based assay for the detection of tumor cells disseminated in the circulation at an early stage of the disease is gaining positive feedback from several lines of research. This method seems able to reduce colorectal cancer mortality and may replace colonoscopy as the most effective means of detecting colonic lesions. Methods In this work, we present a new microarray-based high-throughput screening method to identifying candidate marker mRNAs for the early detection of epithelial cells diluted in peripheral blood cells. This method includes 1. direct comparison of different samples of colonic mucosa and of blood cells to identify consistent epithelial-specific mRNAs from among 20,000 cDNA assayed by microarray slides; 2. identification of candidate marker mRNAs by data analysis, which allowed selection of only 10 putative differentially expressed genes; 3. Selection of some of the most suitable mRNAs (TMEM69, RANBP3 and PRSS22) that were assayed in blood samples from normal subjects and patients with colon cancer as possible markers for the presence of epithelial cells in the blood, using reverse transcription – polymerase chain reaction (RT-PCR). Results Our present results seem to provide an indication, for the first time obtained by genome-scale screening, that a suitable and consistent colon epithelium mRNA marker may be difficult to identify. Conclusion The design of new approaches to identify such markers is warranted. Circulate Tumor Cell (dpeaa)DE-He213 mRNA Marker (dpeaa)DE-He213 Normal Human Colon (dpeaa)DE-He213 KRT20 mRNA (dpeaa)DE-He213 KRT20 mRNA Expression (dpeaa)DE-He213 Ugolini, Giampaolo aut Rosati, Giancarlo aut Zanotti, Simone aut Lauriola, Mattia aut Montroni, Isacco aut del Governatore, Marco aut Caira, Antonello aut Taffurelli, Mario aut Santini, Donatella aut Coppola, Domenico aut Guidotti, Lia aut Carinci, Paolo aut Strippoli, Pierluigi aut Enthalten in BMC cancer London : BioMed Central, 2001 6(2006), 1 vom: 20. Okt. (DE-627)326643710 (DE-600)2041352-X 1471-2407 nnns volume:6 year:2006 number:1 day:20 month:10 https://dx.doi.org/10.1186/1471-2407-6-250 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2006 1 20 10
allfieldsGer	10.1186/1471-2407-6-250 doi (DE-627)SPR027609340 (SPR)1471-2407-6-250-e DE-627 ger DE-627 rakwb eng Solmi, Rossella verfasserin aut Microarray-based identification and RT-PCR test screening for epithelial-specific mRNAs in peripheral blood of patients with colon cancer 2006 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Solmi et al; licensee BioMed Central Ltd. 2006 Background The efficacy of screening for colorectal cancer using a simple blood-based assay for the detection of tumor cells disseminated in the circulation at an early stage of the disease is gaining positive feedback from several lines of research. This method seems able to reduce colorectal cancer mortality and may replace colonoscopy as the most effective means of detecting colonic lesions. Methods In this work, we present a new microarray-based high-throughput screening method to identifying candidate marker mRNAs for the early detection of epithelial cells diluted in peripheral blood cells. This method includes 1. direct comparison of different samples of colonic mucosa and of blood cells to identify consistent epithelial-specific mRNAs from among 20,000 cDNA assayed by microarray slides; 2. identification of candidate marker mRNAs by data analysis, which allowed selection of only 10 putative differentially expressed genes; 3. Selection of some of the most suitable mRNAs (TMEM69, RANBP3 and PRSS22) that were assayed in blood samples from normal subjects and patients with colon cancer as possible markers for the presence of epithelial cells in the blood, using reverse transcription – polymerase chain reaction (RT-PCR). Results Our present results seem to provide an indication, for the first time obtained by genome-scale screening, that a suitable and consistent colon epithelium mRNA marker may be difficult to identify. Conclusion The design of new approaches to identify such markers is warranted. Circulate Tumor Cell (dpeaa)DE-He213 mRNA Marker (dpeaa)DE-He213 Normal Human Colon (dpeaa)DE-He213 KRT20 mRNA (dpeaa)DE-He213 KRT20 mRNA Expression (dpeaa)DE-He213 Ugolini, Giampaolo aut Rosati, Giancarlo aut Zanotti, Simone aut Lauriola, Mattia aut Montroni, Isacco aut del Governatore, Marco aut Caira, Antonello aut Taffurelli, Mario aut Santini, Donatella aut Coppola, Domenico aut Guidotti, Lia aut Carinci, Paolo aut Strippoli, Pierluigi aut Enthalten in BMC cancer London : BioMed Central, 2001 6(2006), 1 vom: 20. Okt. (DE-627)326643710 (DE-600)2041352-X 1471-2407 nnns volume:6 year:2006 number:1 day:20 month:10 https://dx.doi.org/10.1186/1471-2407-6-250 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2006 1 20 10
allfieldsSound	10.1186/1471-2407-6-250 doi (DE-627)SPR027609340 (SPR)1471-2407-6-250-e DE-627 ger DE-627 rakwb eng Solmi, Rossella verfasserin aut Microarray-based identification and RT-PCR test screening for epithelial-specific mRNAs in peripheral blood of patients with colon cancer 2006 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Solmi et al; licensee BioMed Central Ltd. 2006 Background The efficacy of screening for colorectal cancer using a simple blood-based assay for the detection of tumor cells disseminated in the circulation at an early stage of the disease is gaining positive feedback from several lines of research. This method seems able to reduce colorectal cancer mortality and may replace colonoscopy as the most effective means of detecting colonic lesions. Methods In this work, we present a new microarray-based high-throughput screening method to identifying candidate marker mRNAs for the early detection of epithelial cells diluted in peripheral blood cells. This method includes 1. direct comparison of different samples of colonic mucosa and of blood cells to identify consistent epithelial-specific mRNAs from among 20,000 cDNA assayed by microarray slides; 2. identification of candidate marker mRNAs by data analysis, which allowed selection of only 10 putative differentially expressed genes; 3. Selection of some of the most suitable mRNAs (TMEM69, RANBP3 and PRSS22) that were assayed in blood samples from normal subjects and patients with colon cancer as possible markers for the presence of epithelial cells in the blood, using reverse transcription – polymerase chain reaction (RT-PCR). Results Our present results seem to provide an indication, for the first time obtained by genome-scale screening, that a suitable and consistent colon epithelium mRNA marker may be difficult to identify. Conclusion The design of new approaches to identify such markers is warranted. Circulate Tumor Cell (dpeaa)DE-He213 mRNA Marker (dpeaa)DE-He213 Normal Human Colon (dpeaa)DE-He213 KRT20 mRNA (dpeaa)DE-He213 KRT20 mRNA Expression (dpeaa)DE-He213 Ugolini, Giampaolo aut Rosati, Giancarlo aut Zanotti, Simone aut Lauriola, Mattia aut Montroni, Isacco aut del Governatore, Marco aut Caira, Antonello aut Taffurelli, Mario aut Santini, Donatella aut Coppola, Domenico aut Guidotti, Lia aut Carinci, Paolo aut Strippoli, Pierluigi aut Enthalten in BMC cancer London : BioMed Central, 2001 6(2006), 1 vom: 20. Okt. (DE-627)326643710 (DE-600)2041352-X 1471-2407 nnns volume:6 year:2006 number:1 day:20 month:10 https://dx.doi.org/10.1186/1471-2407-6-250 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2006 1 20 10
language	English
source	Enthalten in BMC cancer 6(2006), 1 vom: 20. Okt. volume:6 year:2006 number:1 day:20 month:10
sourceStr	Enthalten in BMC cancer 6(2006), 1 vom: 20. Okt. volume:6 year:2006 number:1 day:20 month:10
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Circulate Tumor Cell mRNA Marker Normal Human Colon KRT20 mRNA KRT20 mRNA Expression
isfreeaccess_bool	true
container_title	BMC cancer
authorswithroles_txt_mv	Solmi, Rossella @@aut@@ Ugolini, Giampaolo @@aut@@ Rosati, Giancarlo @@aut@@ Zanotti, Simone @@aut@@ Lauriola, Mattia @@aut@@ Montroni, Isacco @@aut@@ del Governatore, Marco @@aut@@ Caira, Antonello @@aut@@ Taffurelli, Mario @@aut@@ Santini, Donatella @@aut@@ Coppola, Domenico @@aut@@ Guidotti, Lia @@aut@@ Carinci, Paolo @@aut@@ Strippoli, Pierluigi @@aut@@
publishDateDaySort_date	2006-10-20T00:00:00Z
hierarchy_top_id	326643710
id	SPR027609340
language_de	englisch
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author	Solmi, Rossella
spellingShingle	Solmi, Rossella misc Circulate Tumor Cell misc mRNA Marker misc Normal Human Colon misc KRT20 mRNA misc KRT20 mRNA Expression Microarray-based identification and RT-PCR test screening for epithelial-specific mRNAs in peripheral blood of patients with colon cancer
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topic_title	Microarray-based identification and RT-PCR test screening for epithelial-specific mRNAs in peripheral blood of patients with colon cancer Circulate Tumor Cell (dpeaa)DE-He213 mRNA Marker (dpeaa)DE-He213 Normal Human Colon (dpeaa)DE-He213 KRT20 mRNA (dpeaa)DE-He213 KRT20 mRNA Expression (dpeaa)DE-He213
topic	misc Circulate Tumor Cell misc mRNA Marker misc Normal Human Colon misc KRT20 mRNA misc KRT20 mRNA Expression
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title	Microarray-based identification and RT-PCR test screening for epithelial-specific mRNAs in peripheral blood of patients with colon cancer
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title_full	Microarray-based identification and RT-PCR test screening for epithelial-specific mRNAs in peripheral blood of patients with colon cancer
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author_browse	Solmi, Rossella Ugolini, Giampaolo Rosati, Giancarlo Zanotti, Simone Lauriola, Mattia Montroni, Isacco del Governatore, Marco Caira, Antonello Taffurelli, Mario Santini, Donatella Coppola, Domenico Guidotti, Lia Carinci, Paolo Strippoli, Pierluigi
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doi_str_mv	10.1186/1471-2407-6-250
title_sort	microarray-based identification and rt-pcr test screening for epithelial-specific mrnas in peripheral blood of patients with colon cancer
title_auth	Microarray-based identification and RT-PCR test screening for epithelial-specific mRNAs in peripheral blood of patients with colon cancer
abstract	Background The efficacy of screening for colorectal cancer using a simple blood-based assay for the detection of tumor cells disseminated in the circulation at an early stage of the disease is gaining positive feedback from several lines of research. This method seems able to reduce colorectal cancer mortality and may replace colonoscopy as the most effective means of detecting colonic lesions. Methods In this work, we present a new microarray-based high-throughput screening method to identifying candidate marker mRNAs for the early detection of epithelial cells diluted in peripheral blood cells. This method includes 1. direct comparison of different samples of colonic mucosa and of blood cells to identify consistent epithelial-specific mRNAs from among 20,000 cDNA assayed by microarray slides; 2. identification of candidate marker mRNAs by data analysis, which allowed selection of only 10 putative differentially expressed genes; 3. Selection of some of the most suitable mRNAs (TMEM69, RANBP3 and PRSS22) that were assayed in blood samples from normal subjects and patients with colon cancer as possible markers for the presence of epithelial cells in the blood, using reverse transcription – polymerase chain reaction (RT-PCR). Results Our present results seem to provide an indication, for the first time obtained by genome-scale screening, that a suitable and consistent colon epithelium mRNA marker may be difficult to identify. Conclusion The design of new approaches to identify such markers is warranted. © Solmi et al; licensee BioMed Central Ltd. 2006
abstractGer	Background The efficacy of screening for colorectal cancer using a simple blood-based assay for the detection of tumor cells disseminated in the circulation at an early stage of the disease is gaining positive feedback from several lines of research. This method seems able to reduce colorectal cancer mortality and may replace colonoscopy as the most effective means of detecting colonic lesions. Methods In this work, we present a new microarray-based high-throughput screening method to identifying candidate marker mRNAs for the early detection of epithelial cells diluted in peripheral blood cells. This method includes 1. direct comparison of different samples of colonic mucosa and of blood cells to identify consistent epithelial-specific mRNAs from among 20,000 cDNA assayed by microarray slides; 2. identification of candidate marker mRNAs by data analysis, which allowed selection of only 10 putative differentially expressed genes; 3. Selection of some of the most suitable mRNAs (TMEM69, RANBP3 and PRSS22) that were assayed in blood samples from normal subjects and patients with colon cancer as possible markers for the presence of epithelial cells in the blood, using reverse transcription – polymerase chain reaction (RT-PCR). Results Our present results seem to provide an indication, for the first time obtained by genome-scale screening, that a suitable and consistent colon epithelium mRNA marker may be difficult to identify. Conclusion The design of new approaches to identify such markers is warranted. © Solmi et al; licensee BioMed Central Ltd. 2006
abstract_unstemmed	Background The efficacy of screening for colorectal cancer using a simple blood-based assay for the detection of tumor cells disseminated in the circulation at an early stage of the disease is gaining positive feedback from several lines of research. This method seems able to reduce colorectal cancer mortality and may replace colonoscopy as the most effective means of detecting colonic lesions. Methods In this work, we present a new microarray-based high-throughput screening method to identifying candidate marker mRNAs for the early detection of epithelial cells diluted in peripheral blood cells. This method includes 1. direct comparison of different samples of colonic mucosa and of blood cells to identify consistent epithelial-specific mRNAs from among 20,000 cDNA assayed by microarray slides; 2. identification of candidate marker mRNAs by data analysis, which allowed selection of only 10 putative differentially expressed genes; 3. Selection of some of the most suitable mRNAs (TMEM69, RANBP3 and PRSS22) that were assayed in blood samples from normal subjects and patients with colon cancer as possible markers for the presence of epithelial cells in the blood, using reverse transcription – polymerase chain reaction (RT-PCR). Results Our present results seem to provide an indication, for the first time obtained by genome-scale screening, that a suitable and consistent colon epithelium mRNA marker may be difficult to identify. Conclusion The design of new approaches to identify such markers is warranted. © Solmi et al; licensee BioMed Central Ltd. 2006
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title_short	Microarray-based identification and RT-PCR test screening for epithelial-specific mRNAs in peripheral blood of patients with colon cancer
url	https://dx.doi.org/10.1186/1471-2407-6-250
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author2	Ugolini, Giampaolo Rosati, Giancarlo Zanotti, Simone Lauriola, Mattia Montroni, Isacco del Governatore, Marco Caira, Antonello Taffurelli, Mario Santini, Donatella Coppola, Domenico Guidotti, Lia Carinci, Paolo Strippoli, Pierluigi
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Microarray-based identification and RT-PCR test screening for epithelial-specific mRNAs in peripheral blood of patients with colon cancer

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