Hematopoietic chimerism after allogeneic stem cell transplantation: a comparison of quantitative analysis by automated DNA sizing and fluorescent in situhybridization
Background Allogeneic hematopoietic stem cell transplantation (allo-HSCT) is performed mainly in patients with high-risk or advanced hematologic malignancies and congenital or acquired aplastic anemias. In the context of the significant risk of graft failure after allo-HSCT from alternative donors a...
Ausführliche Beschreibung
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Jółkowska, Justyna [verfasserIn] |
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© Jółkowska et al; licensee BioMed Central Ltd. 2005. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( |
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Übergeordnetes Werk: |
Enthalten in: BMC hematology - London : BioMed Central, 2013, 5(2005), 1 vom: 10. Jan. |
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Übergeordnetes Werk: |
volume:5 ; year:2005 ; number:1 ; day:10 ; month:01 |
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DOI / URN: |
10.1186/1471-2326-5-1 |
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SPR027638987 |
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520 | |a Background Allogeneic hematopoietic stem cell transplantation (allo-HSCT) is performed mainly in patients with high-risk or advanced hematologic malignancies and congenital or acquired aplastic anemias. In the context of the significant risk of graft failure after allo-HSCT from alternative donors and the risk of relapse in recipients transplanted for malignancy, the precise monitoring of posttransplant hematopoietic chimerism is of utmost interest. Useful molecular methods for chimerism quantification after allogeneic transplantation, aimed at distinguishing precisely between donor's and recipient's cells, are PCR-based analyses of polymorphic DNA markers. Such analyses can be performed regardless of donor's and recipient's sex. Additionally, in patients after sex-mismatched allo-HSCT, fluorescent in situ hybridization (FISH) can be applied. Methods We compared different techniques for analysis of posttransplant chimerism, namely FISH and PCR-based molecular methods with automated detection of fluorescent products in an ALFExpress DNA Sequencer (Pharmacia) or ABI 310 Genetic Analyzer (PE). We used Spearman correlation test. Results We have found high correlation between results obtained from the PCR/ALF Express and PCR/ABI 310 Genetic Analyzer. Lower, but still positive correlations were found between results of FISH technique and results obtained using automated DNA sizing technology. Conclusions All the methods applied enable a rapid and accurate detection of post-HSCT chimerism. | ||
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700 | 1 | |a Pieczonka, Anna |4 aut | |
700 | 1 | |a Strabel, Tomasz |4 aut | |
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10.1186/1471-2326-5-1 doi (DE-627)SPR027638987 (SPR)1471-2326-5-1-e DE-627 ger DE-627 rakwb eng Jółkowska, Justyna verfasserin aut Hematopoietic chimerism after allogeneic stem cell transplantation: a comparison of quantitative analysis by automated DNA sizing and fluorescent in situhybridization 2005 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Jółkowska et al; licensee BioMed Central Ltd. 2005. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( Background Allogeneic hematopoietic stem cell transplantation (allo-HSCT) is performed mainly in patients with high-risk or advanced hematologic malignancies and congenital or acquired aplastic anemias. In the context of the significant risk of graft failure after allo-HSCT from alternative donors and the risk of relapse in recipients transplanted for malignancy, the precise monitoring of posttransplant hematopoietic chimerism is of utmost interest. Useful molecular methods for chimerism quantification after allogeneic transplantation, aimed at distinguishing precisely between donor's and recipient's cells, are PCR-based analyses of polymorphic DNA markers. Such analyses can be performed regardless of donor's and recipient's sex. Additionally, in patients after sex-mismatched allo-HSCT, fluorescent in situ hybridization (FISH) can be applied. Methods We compared different techniques for analysis of posttransplant chimerism, namely FISH and PCR-based molecular methods with automated detection of fluorescent products in an ALFExpress DNA Sequencer (Pharmacia) or ABI 310 Genetic Analyzer (PE). We used Spearman correlation test. Results We have found high correlation between results obtained from the PCR/ALF Express and PCR/ABI 310 Genetic Analyzer. Lower, but still positive correlations were found between results of FISH technique and results obtained using automated DNA sizing technology. Conclusions All the methods applied enable a rapid and accurate detection of post-HSCT chimerism. Allogeneic Hematopoietic Stem Cell Transplantation (dpeaa)DE-He213 Short Tandem Repeat Locus (dpeaa)DE-He213 Mixed Chimerism (dpeaa)DE-He213 Hematopoietic Chimerism (dpeaa)DE-He213 Complete Chimerism (dpeaa)DE-He213 Pieczonka, Anna aut Strabel, Tomasz aut Boruczkowski, Dariusz aut Wachowiak, Jacek aut Bader, Peter aut Witt, Michał aut Enthalten in BMC hematology London : BioMed Central, 2013 5(2005), 1 vom: 10. Jan. (DE-627)773473114 (DE-600)2744433-8 2052-1839 nnns volume:5 year:2005 number:1 day:10 month:01 https://dx.doi.org/10.1186/1471-2326-5-1 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_95 GBV_ILN_110 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 AR 5 2005 1 10 01 |
spelling |
10.1186/1471-2326-5-1 doi (DE-627)SPR027638987 (SPR)1471-2326-5-1-e DE-627 ger DE-627 rakwb eng Jółkowska, Justyna verfasserin aut Hematopoietic chimerism after allogeneic stem cell transplantation: a comparison of quantitative analysis by automated DNA sizing and fluorescent in situhybridization 2005 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Jółkowska et al; licensee BioMed Central Ltd. 2005. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( Background Allogeneic hematopoietic stem cell transplantation (allo-HSCT) is performed mainly in patients with high-risk or advanced hematologic malignancies and congenital or acquired aplastic anemias. In the context of the significant risk of graft failure after allo-HSCT from alternative donors and the risk of relapse in recipients transplanted for malignancy, the precise monitoring of posttransplant hematopoietic chimerism is of utmost interest. Useful molecular methods for chimerism quantification after allogeneic transplantation, aimed at distinguishing precisely between donor's and recipient's cells, are PCR-based analyses of polymorphic DNA markers. Such analyses can be performed regardless of donor's and recipient's sex. Additionally, in patients after sex-mismatched allo-HSCT, fluorescent in situ hybridization (FISH) can be applied. Methods We compared different techniques for analysis of posttransplant chimerism, namely FISH and PCR-based molecular methods with automated detection of fluorescent products in an ALFExpress DNA Sequencer (Pharmacia) or ABI 310 Genetic Analyzer (PE). We used Spearman correlation test. Results We have found high correlation between results obtained from the PCR/ALF Express and PCR/ABI 310 Genetic Analyzer. Lower, but still positive correlations were found between results of FISH technique and results obtained using automated DNA sizing technology. Conclusions All the methods applied enable a rapid and accurate detection of post-HSCT chimerism. Allogeneic Hematopoietic Stem Cell Transplantation (dpeaa)DE-He213 Short Tandem Repeat Locus (dpeaa)DE-He213 Mixed Chimerism (dpeaa)DE-He213 Hematopoietic Chimerism (dpeaa)DE-He213 Complete Chimerism (dpeaa)DE-He213 Pieczonka, Anna aut Strabel, Tomasz aut Boruczkowski, Dariusz aut Wachowiak, Jacek aut Bader, Peter aut Witt, Michał aut Enthalten in BMC hematology London : BioMed Central, 2013 5(2005), 1 vom: 10. Jan. (DE-627)773473114 (DE-600)2744433-8 2052-1839 nnns volume:5 year:2005 number:1 day:10 month:01 https://dx.doi.org/10.1186/1471-2326-5-1 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_95 GBV_ILN_110 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 AR 5 2005 1 10 01 |
allfields_unstemmed |
10.1186/1471-2326-5-1 doi (DE-627)SPR027638987 (SPR)1471-2326-5-1-e DE-627 ger DE-627 rakwb eng Jółkowska, Justyna verfasserin aut Hematopoietic chimerism after allogeneic stem cell transplantation: a comparison of quantitative analysis by automated DNA sizing and fluorescent in situhybridization 2005 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Jółkowska et al; licensee BioMed Central Ltd. 2005. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( Background Allogeneic hematopoietic stem cell transplantation (allo-HSCT) is performed mainly in patients with high-risk or advanced hematologic malignancies and congenital or acquired aplastic anemias. In the context of the significant risk of graft failure after allo-HSCT from alternative donors and the risk of relapse in recipients transplanted for malignancy, the precise monitoring of posttransplant hematopoietic chimerism is of utmost interest. Useful molecular methods for chimerism quantification after allogeneic transplantation, aimed at distinguishing precisely between donor's and recipient's cells, are PCR-based analyses of polymorphic DNA markers. Such analyses can be performed regardless of donor's and recipient's sex. Additionally, in patients after sex-mismatched allo-HSCT, fluorescent in situ hybridization (FISH) can be applied. Methods We compared different techniques for analysis of posttransplant chimerism, namely FISH and PCR-based molecular methods with automated detection of fluorescent products in an ALFExpress DNA Sequencer (Pharmacia) or ABI 310 Genetic Analyzer (PE). We used Spearman correlation test. Results We have found high correlation between results obtained from the PCR/ALF Express and PCR/ABI 310 Genetic Analyzer. Lower, but still positive correlations were found between results of FISH technique and results obtained using automated DNA sizing technology. Conclusions All the methods applied enable a rapid and accurate detection of post-HSCT chimerism. Allogeneic Hematopoietic Stem Cell Transplantation (dpeaa)DE-He213 Short Tandem Repeat Locus (dpeaa)DE-He213 Mixed Chimerism (dpeaa)DE-He213 Hematopoietic Chimerism (dpeaa)DE-He213 Complete Chimerism (dpeaa)DE-He213 Pieczonka, Anna aut Strabel, Tomasz aut Boruczkowski, Dariusz aut Wachowiak, Jacek aut Bader, Peter aut Witt, Michał aut Enthalten in BMC hematology London : BioMed Central, 2013 5(2005), 1 vom: 10. Jan. (DE-627)773473114 (DE-600)2744433-8 2052-1839 nnns volume:5 year:2005 number:1 day:10 month:01 https://dx.doi.org/10.1186/1471-2326-5-1 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_95 GBV_ILN_110 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 AR 5 2005 1 10 01 |
allfieldsGer |
10.1186/1471-2326-5-1 doi (DE-627)SPR027638987 (SPR)1471-2326-5-1-e DE-627 ger DE-627 rakwb eng Jółkowska, Justyna verfasserin aut Hematopoietic chimerism after allogeneic stem cell transplantation: a comparison of quantitative analysis by automated DNA sizing and fluorescent in situhybridization 2005 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Jółkowska et al; licensee BioMed Central Ltd. 2005. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( Background Allogeneic hematopoietic stem cell transplantation (allo-HSCT) is performed mainly in patients with high-risk or advanced hematologic malignancies and congenital or acquired aplastic anemias. In the context of the significant risk of graft failure after allo-HSCT from alternative donors and the risk of relapse in recipients transplanted for malignancy, the precise monitoring of posttransplant hematopoietic chimerism is of utmost interest. Useful molecular methods for chimerism quantification after allogeneic transplantation, aimed at distinguishing precisely between donor's and recipient's cells, are PCR-based analyses of polymorphic DNA markers. Such analyses can be performed regardless of donor's and recipient's sex. Additionally, in patients after sex-mismatched allo-HSCT, fluorescent in situ hybridization (FISH) can be applied. Methods We compared different techniques for analysis of posttransplant chimerism, namely FISH and PCR-based molecular methods with automated detection of fluorescent products in an ALFExpress DNA Sequencer (Pharmacia) or ABI 310 Genetic Analyzer (PE). We used Spearman correlation test. Results We have found high correlation between results obtained from the PCR/ALF Express and PCR/ABI 310 Genetic Analyzer. Lower, but still positive correlations were found between results of FISH technique and results obtained using automated DNA sizing technology. Conclusions All the methods applied enable a rapid and accurate detection of post-HSCT chimerism. Allogeneic Hematopoietic Stem Cell Transplantation (dpeaa)DE-He213 Short Tandem Repeat Locus (dpeaa)DE-He213 Mixed Chimerism (dpeaa)DE-He213 Hematopoietic Chimerism (dpeaa)DE-He213 Complete Chimerism (dpeaa)DE-He213 Pieczonka, Anna aut Strabel, Tomasz aut Boruczkowski, Dariusz aut Wachowiak, Jacek aut Bader, Peter aut Witt, Michał aut Enthalten in BMC hematology London : BioMed Central, 2013 5(2005), 1 vom: 10. Jan. (DE-627)773473114 (DE-600)2744433-8 2052-1839 nnns volume:5 year:2005 number:1 day:10 month:01 https://dx.doi.org/10.1186/1471-2326-5-1 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_95 GBV_ILN_110 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 AR 5 2005 1 10 01 |
allfieldsSound |
10.1186/1471-2326-5-1 doi (DE-627)SPR027638987 (SPR)1471-2326-5-1-e DE-627 ger DE-627 rakwb eng Jółkowska, Justyna verfasserin aut Hematopoietic chimerism after allogeneic stem cell transplantation: a comparison of quantitative analysis by automated DNA sizing and fluorescent in situhybridization 2005 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Jółkowska et al; licensee BioMed Central Ltd. 2005. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( Background Allogeneic hematopoietic stem cell transplantation (allo-HSCT) is performed mainly in patients with high-risk or advanced hematologic malignancies and congenital or acquired aplastic anemias. In the context of the significant risk of graft failure after allo-HSCT from alternative donors and the risk of relapse in recipients transplanted for malignancy, the precise monitoring of posttransplant hematopoietic chimerism is of utmost interest. Useful molecular methods for chimerism quantification after allogeneic transplantation, aimed at distinguishing precisely between donor's and recipient's cells, are PCR-based analyses of polymorphic DNA markers. Such analyses can be performed regardless of donor's and recipient's sex. Additionally, in patients after sex-mismatched allo-HSCT, fluorescent in situ hybridization (FISH) can be applied. Methods We compared different techniques for analysis of posttransplant chimerism, namely FISH and PCR-based molecular methods with automated detection of fluorescent products in an ALFExpress DNA Sequencer (Pharmacia) or ABI 310 Genetic Analyzer (PE). We used Spearman correlation test. Results We have found high correlation between results obtained from the PCR/ALF Express and PCR/ABI 310 Genetic Analyzer. Lower, but still positive correlations were found between results of FISH technique and results obtained using automated DNA sizing technology. Conclusions All the methods applied enable a rapid and accurate detection of post-HSCT chimerism. Allogeneic Hematopoietic Stem Cell Transplantation (dpeaa)DE-He213 Short Tandem Repeat Locus (dpeaa)DE-He213 Mixed Chimerism (dpeaa)DE-He213 Hematopoietic Chimerism (dpeaa)DE-He213 Complete Chimerism (dpeaa)DE-He213 Pieczonka, Anna aut Strabel, Tomasz aut Boruczkowski, Dariusz aut Wachowiak, Jacek aut Bader, Peter aut Witt, Michał aut Enthalten in BMC hematology London : BioMed Central, 2013 5(2005), 1 vom: 10. Jan. (DE-627)773473114 (DE-600)2744433-8 2052-1839 nnns volume:5 year:2005 number:1 day:10 month:01 https://dx.doi.org/10.1186/1471-2326-5-1 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_95 GBV_ILN_110 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 AR 5 2005 1 10 01 |
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Jółkowska, Justyna misc Allogeneic Hematopoietic Stem Cell Transplantation misc Short Tandem Repeat Locus misc Mixed Chimerism misc Hematopoietic Chimerism misc Complete Chimerism Hematopoietic chimerism after allogeneic stem cell transplantation: a comparison of quantitative analysis by automated DNA sizing and fluorescent in situhybridization |
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Hematopoietic chimerism after allogeneic stem cell transplantation: a comparison of quantitative analysis by automated DNA sizing and fluorescent in situhybridization Allogeneic Hematopoietic Stem Cell Transplantation (dpeaa)DE-He213 Short Tandem Repeat Locus (dpeaa)DE-He213 Mixed Chimerism (dpeaa)DE-He213 Hematopoietic Chimerism (dpeaa)DE-He213 Complete Chimerism (dpeaa)DE-He213 |
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hematopoietic chimerism after allogeneic stem cell transplantation: a comparison of quantitative analysis by automated dna sizing and fluorescent in situhybridization |
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Hematopoietic chimerism after allogeneic stem cell transplantation: a comparison of quantitative analysis by automated DNA sizing and fluorescent in situhybridization |
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Background Allogeneic hematopoietic stem cell transplantation (allo-HSCT) is performed mainly in patients with high-risk or advanced hematologic malignancies and congenital or acquired aplastic anemias. In the context of the significant risk of graft failure after allo-HSCT from alternative donors and the risk of relapse in recipients transplanted for malignancy, the precise monitoring of posttransplant hematopoietic chimerism is of utmost interest. Useful molecular methods for chimerism quantification after allogeneic transplantation, aimed at distinguishing precisely between donor's and recipient's cells, are PCR-based analyses of polymorphic DNA markers. Such analyses can be performed regardless of donor's and recipient's sex. Additionally, in patients after sex-mismatched allo-HSCT, fluorescent in situ hybridization (FISH) can be applied. Methods We compared different techniques for analysis of posttransplant chimerism, namely FISH and PCR-based molecular methods with automated detection of fluorescent products in an ALFExpress DNA Sequencer (Pharmacia) or ABI 310 Genetic Analyzer (PE). We used Spearman correlation test. Results We have found high correlation between results obtained from the PCR/ALF Express and PCR/ABI 310 Genetic Analyzer. Lower, but still positive correlations were found between results of FISH technique and results obtained using automated DNA sizing technology. Conclusions All the methods applied enable a rapid and accurate detection of post-HSCT chimerism. © Jółkowska et al; licensee BioMed Central Ltd. 2005. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( |
abstractGer |
Background Allogeneic hematopoietic stem cell transplantation (allo-HSCT) is performed mainly in patients with high-risk or advanced hematologic malignancies and congenital or acquired aplastic anemias. In the context of the significant risk of graft failure after allo-HSCT from alternative donors and the risk of relapse in recipients transplanted for malignancy, the precise monitoring of posttransplant hematopoietic chimerism is of utmost interest. Useful molecular methods for chimerism quantification after allogeneic transplantation, aimed at distinguishing precisely between donor's and recipient's cells, are PCR-based analyses of polymorphic DNA markers. Such analyses can be performed regardless of donor's and recipient's sex. Additionally, in patients after sex-mismatched allo-HSCT, fluorescent in situ hybridization (FISH) can be applied. Methods We compared different techniques for analysis of posttransplant chimerism, namely FISH and PCR-based molecular methods with automated detection of fluorescent products in an ALFExpress DNA Sequencer (Pharmacia) or ABI 310 Genetic Analyzer (PE). We used Spearman correlation test. Results We have found high correlation between results obtained from the PCR/ALF Express and PCR/ABI 310 Genetic Analyzer. Lower, but still positive correlations were found between results of FISH technique and results obtained using automated DNA sizing technology. Conclusions All the methods applied enable a rapid and accurate detection of post-HSCT chimerism. © Jółkowska et al; licensee BioMed Central Ltd. 2005. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( |
abstract_unstemmed |
Background Allogeneic hematopoietic stem cell transplantation (allo-HSCT) is performed mainly in patients with high-risk or advanced hematologic malignancies and congenital or acquired aplastic anemias. In the context of the significant risk of graft failure after allo-HSCT from alternative donors and the risk of relapse in recipients transplanted for malignancy, the precise monitoring of posttransplant hematopoietic chimerism is of utmost interest. Useful molecular methods for chimerism quantification after allogeneic transplantation, aimed at distinguishing precisely between donor's and recipient's cells, are PCR-based analyses of polymorphic DNA markers. Such analyses can be performed regardless of donor's and recipient's sex. Additionally, in patients after sex-mismatched allo-HSCT, fluorescent in situ hybridization (FISH) can be applied. Methods We compared different techniques for analysis of posttransplant chimerism, namely FISH and PCR-based molecular methods with automated detection of fluorescent products in an ALFExpress DNA Sequencer (Pharmacia) or ABI 310 Genetic Analyzer (PE). We used Spearman correlation test. Results We have found high correlation between results obtained from the PCR/ALF Express and PCR/ABI 310 Genetic Analyzer. Lower, but still positive correlations were found between results of FISH technique and results obtained using automated DNA sizing technology. Conclusions All the methods applied enable a rapid and accurate detection of post-HSCT chimerism. © Jółkowska et al; licensee BioMed Central Ltd. 2005. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( |
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