SISH/CISH or qPCR as alternative techniques to FISH for determination of HER2 amplification status on breast tumors core needle biopsies: a multicenter experience based on 840 cases

Background Until now, FISH has been the gold standard technique to identify HER2 amplification status in ambiguous cases of breast cancer. Alternative techniques have been developed to increase the capacities of investigating HER2 amplification status. The aims of this multicenter study in a large series of breast cancer patients were to prospectively compare the level of performance of CISH, SISH, and qPCR alternative techniques on paraffin-embedded core biopsies with “gold standard FISH” for evaluation of HER2 amplification status. Methods This study was performed on 840 cases scored by immunohistochemistry (IHC): 0=317 (38%), 1+=183 (22%), 2+=109 (13%), 3+=231 (27%). Each of the 15 French centers participating in the study analyzed 56 breast carcinoma cases diagnosed on fixed paraffin-embedded core biopsies. HER2 amplification status was determined by commercially available FISH used as the reference technique with determination of the HER2/CEN17 ratio or HER2 copy number status. The alternative techniques performed on the same cases were commercially available SISH or CISH and a common qPCR method especially designed for the study including a set of 10 primer pairs: 2 for HER2 (exons 8 and 26), 5 to evaluate chromosome 17 polysomy TAOK1, UTP6, MRM1, MKS1, SSTR2 and 3 for diploidy control TSN, LAP3 and ADAMTS16. Results The concordance between IHC and FISH was 96% to 95% based on the HER2/CEN17 ratio (n=766) or HER2 copy number (n=840), respectively. The concordance of the alternative techniques with FISH was excellent: 97% and 98% for SISH (498 and 587 cases), 98% and 75% for CISH (108 and 204 cases) and 95% and 93% (699 and 773 cases) for qPCR based on the HER2/CEN17 ratio or HER2 copy number, respectively. Similarly, sensitivity ranged from 99% to 95% for SISH, 100% to 99% for CISH and 89% to 80% for qPCR. The concordance with FISH (ratio) in the 2+ cases was 89% for SISH, 100% for CISH and 93% for qPCR. Conclusion These alternative techniques showed an excellent concordance with FISH in core biopsies allowing their use in routine clinical practice. This newly designed qPCR on paraffin-embedded core biopsies deserves special attention, as it is reliable, easy to perform and less expensive than ISH tests. Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Jacquemier, Jocelyne [verfasserIn] Spyratos, Frédérique Esterni, Benjamin Mozziconacci, Marie-Joëlle Antoine, Martine Arnould, Laurent Lizard, Sarab Bertheau, Philippe Lehmann-Che, Jacqueline Fournier, Cécile Blanc Krieger, Sophie Bibeau, Frédéric Lamy, Pierre-Jean Chenard, Marie Pierre Legrain, Michèle Guinebretière, Jean-Marc Loussouarn, Delphine MacGrogan, Gaëtan Hostein, Isabelle Mathieu, Marie Christine Lacroix, Ludovic Valent, Alexander Robin, Yves Marie Revillion, Françoise Triki, Magali Lacroix Seaume, Aline Salomon, Anne Vincent de Cremoux, Patricia Portefaix, Geneviève Xerri, Luc Vacher, Sophie Bièche, Ivan Penault-Llorca, Frédérique

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2013

Schlagwörter:	HER2 FISH SISH CISH qPCR Multicenter analysis

Anmerkung:	© Jacquemier et al.; licensee BioMed Central Ltd. 2013

Übergeordnetes Werk:	Enthalten in: BMC cancer - London : BioMed Central, 2001, 13(2013), 1 vom: 22. Juli
Übergeordnetes Werk:	volume:13 ; year:2013 ; number:1 ; day:22 ; month:07

Links:	Volltext

DOI / URN:	10.1186/1471-2407-13-351

Katalog-ID:	SPR027647064

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100	1		\|a Jacquemier, Jocelyne \|e verfasserin \|4 aut
245	1	0	\|a SISH/CISH or qPCR as alternative techniques to FISH for determination of HER2 amplification status on breast tumors core needle biopsies: a multicenter experience based on 840 cases
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520			\|a Background Until now, FISH has been the gold standard technique to identify HER2 amplification status in ambiguous cases of breast cancer. Alternative techniques have been developed to increase the capacities of investigating HER2 amplification status. The aims of this multicenter study in a large series of breast cancer patients were to prospectively compare the level of performance of CISH, SISH, and qPCR alternative techniques on paraffin-embedded core biopsies with “gold standard FISH” for evaluation of HER2 amplification status. Methods This study was performed on 840 cases scored by immunohistochemistry (IHC): 0=317 (38%), 1+=183 (22%), 2+=109 (13%), 3+=231 (27%). Each of the 15 French centers participating in the study analyzed 56 breast carcinoma cases diagnosed on fixed paraffin-embedded core biopsies. HER2 amplification status was determined by commercially available FISH used as the reference technique with determination of the HER2/CEN17 ratio or HER2 copy number status. The alternative techniques performed on the same cases were commercially available SISH or CISH and a common qPCR method especially designed for the study including a set of 10 primer pairs: 2 for HER2 (exons 8 and 26), 5 to evaluate chromosome 17 polysomy TAOK1, UTP6, MRM1, MKS1, SSTR2 and 3 for diploidy control TSN, LAP3 and ADAMTS16. Results The concordance between IHC and FISH was 96% to 95% based on the HER2/CEN17 ratio (n=766) or HER2 copy number (n=840), respectively. The concordance of the alternative techniques with FISH was excellent: 97% and 98% for SISH (498 and 587 cases), 98% and 75% for CISH (108 and 204 cases) and 95% and 93% (699 and 773 cases) for qPCR based on the HER2/CEN17 ratio or HER2 copy number, respectively. Similarly, sensitivity ranged from 99% to 95% for SISH, 100% to 99% for CISH and 89% to 80% for qPCR. The concordance with FISH (ratio) in the 2+ cases was 89% for SISH, 100% for CISH and 93% for qPCR. Conclusion These alternative techniques showed an excellent concordance with FISH in core biopsies allowing their use in routine clinical practice. This newly designed qPCR on paraffin-embedded core biopsies deserves special attention, as it is reliable, easy to perform and less expensive than ISH tests.
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allfields	10.1186/1471-2407-13-351 doi (DE-627)SPR027647064 (SPR)1471-2407-13-351-e DE-627 ger DE-627 rakwb eng Jacquemier, Jocelyne verfasserin aut SISH/CISH or qPCR as alternative techniques to FISH for determination of HER2 amplification status on breast tumors core needle biopsies: a multicenter experience based on 840 cases 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Jacquemier et al.; licensee BioMed Central Ltd. 2013 Background Until now, FISH has been the gold standard technique to identify HER2 amplification status in ambiguous cases of breast cancer. Alternative techniques have been developed to increase the capacities of investigating HER2 amplification status. The aims of this multicenter study in a large series of breast cancer patients were to prospectively compare the level of performance of CISH, SISH, and qPCR alternative techniques on paraffin-embedded core biopsies with “gold standard FISH” for evaluation of HER2 amplification status. Methods This study was performed on 840 cases scored by immunohistochemistry (IHC): 0=317 (38%), 1+=183 (22%), 2+=109 (13%), 3+=231 (27%). Each of the 15 French centers participating in the study analyzed 56 breast carcinoma cases diagnosed on fixed paraffin-embedded core biopsies. HER2 amplification status was determined by commercially available FISH used as the reference technique with determination of the HER2/CEN17 ratio or HER2 copy number status. The alternative techniques performed on the same cases were commercially available SISH or CISH and a common qPCR method especially designed for the study including a set of 10 primer pairs: 2 for HER2 (exons 8 and 26), 5 to evaluate chromosome 17 polysomy TAOK1, UTP6, MRM1, MKS1, SSTR2 and 3 for diploidy control TSN, LAP3 and ADAMTS16. Results The concordance between IHC and FISH was 96% to 95% based on the HER2/CEN17 ratio (n=766) or HER2 copy number (n=840), respectively. The concordance of the alternative techniques with FISH was excellent: 97% and 98% for SISH (498 and 587 cases), 98% and 75% for CISH (108 and 204 cases) and 95% and 93% (699 and 773 cases) for qPCR based on the HER2/CEN17 ratio or HER2 copy number, respectively. Similarly, sensitivity ranged from 99% to 95% for SISH, 100% to 99% for CISH and 89% to 80% for qPCR. The concordance with FISH (ratio) in the 2+ cases was 89% for SISH, 100% for CISH and 93% for qPCR. Conclusion These alternative techniques showed an excellent concordance with FISH in core biopsies allowing their use in routine clinical practice. This newly designed qPCR on paraffin-embedded core biopsies deserves special attention, as it is reliable, easy to perform and less expensive than ISH tests. HER2 (dpeaa)DE-He213 FISH (dpeaa)DE-He213 SISH (dpeaa)DE-He213 CISH (dpeaa)DE-He213 qPCR (dpeaa)DE-He213 Multicenter analysis (dpeaa)DE-He213 Spyratos, Frédérique aut Esterni, Benjamin aut Mozziconacci, Marie-Joëlle aut Antoine, Martine aut Arnould, Laurent aut Lizard, Sarab aut Bertheau, Philippe aut Lehmann-Che, Jacqueline aut Fournier, Cécile Blanc aut Krieger, Sophie aut Bibeau, Frédéric aut Lamy, Pierre-Jean aut Chenard, Marie Pierre aut Legrain, Michèle aut Guinebretière, Jean-Marc aut Loussouarn, Delphine aut MacGrogan, Gaëtan aut Hostein, Isabelle aut Mathieu, Marie Christine aut Lacroix, Ludovic aut Valent, Alexander aut Robin, Yves Marie aut Revillion, Françoise aut Triki, Magali Lacroix aut Seaume, Aline aut Salomon, Anne Vincent aut de Cremoux, Patricia aut Portefaix, Geneviève aut Xerri, Luc aut Vacher, Sophie aut Bièche, Ivan aut Penault-Llorca, Frédérique aut Enthalten in BMC cancer London : BioMed Central, 2001 13(2013), 1 vom: 22. Juli (DE-627)326643710 (DE-600)2041352-X 1471-2407 nnns volume:13 year:2013 number:1 day:22 month:07 https://dx.doi.org/10.1186/1471-2407-13-351 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2013 1 22 07
spelling	10.1186/1471-2407-13-351 doi (DE-627)SPR027647064 (SPR)1471-2407-13-351-e DE-627 ger DE-627 rakwb eng Jacquemier, Jocelyne verfasserin aut SISH/CISH or qPCR as alternative techniques to FISH for determination of HER2 amplification status on breast tumors core needle biopsies: a multicenter experience based on 840 cases 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Jacquemier et al.; licensee BioMed Central Ltd. 2013 Background Until now, FISH has been the gold standard technique to identify HER2 amplification status in ambiguous cases of breast cancer. Alternative techniques have been developed to increase the capacities of investigating HER2 amplification status. The aims of this multicenter study in a large series of breast cancer patients were to prospectively compare the level of performance of CISH, SISH, and qPCR alternative techniques on paraffin-embedded core biopsies with “gold standard FISH” for evaluation of HER2 amplification status. Methods This study was performed on 840 cases scored by immunohistochemistry (IHC): 0=317 (38%), 1+=183 (22%), 2+=109 (13%), 3+=231 (27%). Each of the 15 French centers participating in the study analyzed 56 breast carcinoma cases diagnosed on fixed paraffin-embedded core biopsies. HER2 amplification status was determined by commercially available FISH used as the reference technique with determination of the HER2/CEN17 ratio or HER2 copy number status. The alternative techniques performed on the same cases were commercially available SISH or CISH and a common qPCR method especially designed for the study including a set of 10 primer pairs: 2 for HER2 (exons 8 and 26), 5 to evaluate chromosome 17 polysomy TAOK1, UTP6, MRM1, MKS1, SSTR2 and 3 for diploidy control TSN, LAP3 and ADAMTS16. Results The concordance between IHC and FISH was 96% to 95% based on the HER2/CEN17 ratio (n=766) or HER2 copy number (n=840), respectively. The concordance of the alternative techniques with FISH was excellent: 97% and 98% for SISH (498 and 587 cases), 98% and 75% for CISH (108 and 204 cases) and 95% and 93% (699 and 773 cases) for qPCR based on the HER2/CEN17 ratio or HER2 copy number, respectively. Similarly, sensitivity ranged from 99% to 95% for SISH, 100% to 99% for CISH and 89% to 80% for qPCR. The concordance with FISH (ratio) in the 2+ cases was 89% for SISH, 100% for CISH and 93% for qPCR. Conclusion These alternative techniques showed an excellent concordance with FISH in core biopsies allowing their use in routine clinical practice. This newly designed qPCR on paraffin-embedded core biopsies deserves special attention, as it is reliable, easy to perform and less expensive than ISH tests. HER2 (dpeaa)DE-He213 FISH (dpeaa)DE-He213 SISH (dpeaa)DE-He213 CISH (dpeaa)DE-He213 qPCR (dpeaa)DE-He213 Multicenter analysis (dpeaa)DE-He213 Spyratos, Frédérique aut Esterni, Benjamin aut Mozziconacci, Marie-Joëlle aut Antoine, Martine aut Arnould, Laurent aut Lizard, Sarab aut Bertheau, Philippe aut Lehmann-Che, Jacqueline aut Fournier, Cécile Blanc aut Krieger, Sophie aut Bibeau, Frédéric aut Lamy, Pierre-Jean aut Chenard, Marie Pierre aut Legrain, Michèle aut Guinebretière, Jean-Marc aut Loussouarn, Delphine aut MacGrogan, Gaëtan aut Hostein, Isabelle aut Mathieu, Marie Christine aut Lacroix, Ludovic aut Valent, Alexander aut Robin, Yves Marie aut Revillion, Françoise aut Triki, Magali Lacroix aut Seaume, Aline aut Salomon, Anne Vincent aut de Cremoux, Patricia aut Portefaix, Geneviève aut Xerri, Luc aut Vacher, Sophie aut Bièche, Ivan aut Penault-Llorca, Frédérique aut Enthalten in BMC cancer London : BioMed Central, 2001 13(2013), 1 vom: 22. Juli (DE-627)326643710 (DE-600)2041352-X 1471-2407 nnns volume:13 year:2013 number:1 day:22 month:07 https://dx.doi.org/10.1186/1471-2407-13-351 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2013 1 22 07
allfields_unstemmed	10.1186/1471-2407-13-351 doi (DE-627)SPR027647064 (SPR)1471-2407-13-351-e DE-627 ger DE-627 rakwb eng Jacquemier, Jocelyne verfasserin aut SISH/CISH or qPCR as alternative techniques to FISH for determination of HER2 amplification status on breast tumors core needle biopsies: a multicenter experience based on 840 cases 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Jacquemier et al.; licensee BioMed Central Ltd. 2013 Background Until now, FISH has been the gold standard technique to identify HER2 amplification status in ambiguous cases of breast cancer. Alternative techniques have been developed to increase the capacities of investigating HER2 amplification status. The aims of this multicenter study in a large series of breast cancer patients were to prospectively compare the level of performance of CISH, SISH, and qPCR alternative techniques on paraffin-embedded core biopsies with “gold standard FISH” for evaluation of HER2 amplification status. Methods This study was performed on 840 cases scored by immunohistochemistry (IHC): 0=317 (38%), 1+=183 (22%), 2+=109 (13%), 3+=231 (27%). Each of the 15 French centers participating in the study analyzed 56 breast carcinoma cases diagnosed on fixed paraffin-embedded core biopsies. HER2 amplification status was determined by commercially available FISH used as the reference technique with determination of the HER2/CEN17 ratio or HER2 copy number status. The alternative techniques performed on the same cases were commercially available SISH or CISH and a common qPCR method especially designed for the study including a set of 10 primer pairs: 2 for HER2 (exons 8 and 26), 5 to evaluate chromosome 17 polysomy TAOK1, UTP6, MRM1, MKS1, SSTR2 and 3 for diploidy control TSN, LAP3 and ADAMTS16. Results The concordance between IHC and FISH was 96% to 95% based on the HER2/CEN17 ratio (n=766) or HER2 copy number (n=840), respectively. The concordance of the alternative techniques with FISH was excellent: 97% and 98% for SISH (498 and 587 cases), 98% and 75% for CISH (108 and 204 cases) and 95% and 93% (699 and 773 cases) for qPCR based on the HER2/CEN17 ratio or HER2 copy number, respectively. Similarly, sensitivity ranged from 99% to 95% for SISH, 100% to 99% for CISH and 89% to 80% for qPCR. The concordance with FISH (ratio) in the 2+ cases was 89% for SISH, 100% for CISH and 93% for qPCR. Conclusion These alternative techniques showed an excellent concordance with FISH in core biopsies allowing their use in routine clinical practice. This newly designed qPCR on paraffin-embedded core biopsies deserves special attention, as it is reliable, easy to perform and less expensive than ISH tests. HER2 (dpeaa)DE-He213 FISH (dpeaa)DE-He213 SISH (dpeaa)DE-He213 CISH (dpeaa)DE-He213 qPCR (dpeaa)DE-He213 Multicenter analysis (dpeaa)DE-He213 Spyratos, Frédérique aut Esterni, Benjamin aut Mozziconacci, Marie-Joëlle aut Antoine, Martine aut Arnould, Laurent aut Lizard, Sarab aut Bertheau, Philippe aut Lehmann-Che, Jacqueline aut Fournier, Cécile Blanc aut Krieger, Sophie aut Bibeau, Frédéric aut Lamy, Pierre-Jean aut Chenard, Marie Pierre aut Legrain, Michèle aut Guinebretière, Jean-Marc aut Loussouarn, Delphine aut MacGrogan, Gaëtan aut Hostein, Isabelle aut Mathieu, Marie Christine aut Lacroix, Ludovic aut Valent, Alexander aut Robin, Yves Marie aut Revillion, Françoise aut Triki, Magali Lacroix aut Seaume, Aline aut Salomon, Anne Vincent aut de Cremoux, Patricia aut Portefaix, Geneviève aut Xerri, Luc aut Vacher, Sophie aut Bièche, Ivan aut Penault-Llorca, Frédérique aut Enthalten in BMC cancer London : BioMed Central, 2001 13(2013), 1 vom: 22. Juli (DE-627)326643710 (DE-600)2041352-X 1471-2407 nnns volume:13 year:2013 number:1 day:22 month:07 https://dx.doi.org/10.1186/1471-2407-13-351 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2013 1 22 07
allfieldsGer	10.1186/1471-2407-13-351 doi (DE-627)SPR027647064 (SPR)1471-2407-13-351-e DE-627 ger DE-627 rakwb eng Jacquemier, Jocelyne verfasserin aut SISH/CISH or qPCR as alternative techniques to FISH for determination of HER2 amplification status on breast tumors core needle biopsies: a multicenter experience based on 840 cases 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Jacquemier et al.; licensee BioMed Central Ltd. 2013 Background Until now, FISH has been the gold standard technique to identify HER2 amplification status in ambiguous cases of breast cancer. Alternative techniques have been developed to increase the capacities of investigating HER2 amplification status. The aims of this multicenter study in a large series of breast cancer patients were to prospectively compare the level of performance of CISH, SISH, and qPCR alternative techniques on paraffin-embedded core biopsies with “gold standard FISH” for evaluation of HER2 amplification status. Methods This study was performed on 840 cases scored by immunohistochemistry (IHC): 0=317 (38%), 1+=183 (22%), 2+=109 (13%), 3+=231 (27%). Each of the 15 French centers participating in the study analyzed 56 breast carcinoma cases diagnosed on fixed paraffin-embedded core biopsies. HER2 amplification status was determined by commercially available FISH used as the reference technique with determination of the HER2/CEN17 ratio or HER2 copy number status. The alternative techniques performed on the same cases were commercially available SISH or CISH and a common qPCR method especially designed for the study including a set of 10 primer pairs: 2 for HER2 (exons 8 and 26), 5 to evaluate chromosome 17 polysomy TAOK1, UTP6, MRM1, MKS1, SSTR2 and 3 for diploidy control TSN, LAP3 and ADAMTS16. Results The concordance between IHC and FISH was 96% to 95% based on the HER2/CEN17 ratio (n=766) or HER2 copy number (n=840), respectively. The concordance of the alternative techniques with FISH was excellent: 97% and 98% for SISH (498 and 587 cases), 98% and 75% for CISH (108 and 204 cases) and 95% and 93% (699 and 773 cases) for qPCR based on the HER2/CEN17 ratio or HER2 copy number, respectively. Similarly, sensitivity ranged from 99% to 95% for SISH, 100% to 99% for CISH and 89% to 80% for qPCR. The concordance with FISH (ratio) in the 2+ cases was 89% for SISH, 100% for CISH and 93% for qPCR. Conclusion These alternative techniques showed an excellent concordance with FISH in core biopsies allowing their use in routine clinical practice. This newly designed qPCR on paraffin-embedded core biopsies deserves special attention, as it is reliable, easy to perform and less expensive than ISH tests. HER2 (dpeaa)DE-He213 FISH (dpeaa)DE-He213 SISH (dpeaa)DE-He213 CISH (dpeaa)DE-He213 qPCR (dpeaa)DE-He213 Multicenter analysis (dpeaa)DE-He213 Spyratos, Frédérique aut Esterni, Benjamin aut Mozziconacci, Marie-Joëlle aut Antoine, Martine aut Arnould, Laurent aut Lizard, Sarab aut Bertheau, Philippe aut Lehmann-Che, Jacqueline aut Fournier, Cécile Blanc aut Krieger, Sophie aut Bibeau, Frédéric aut Lamy, Pierre-Jean aut Chenard, Marie Pierre aut Legrain, Michèle aut Guinebretière, Jean-Marc aut Loussouarn, Delphine aut MacGrogan, Gaëtan aut Hostein, Isabelle aut Mathieu, Marie Christine aut Lacroix, Ludovic aut Valent, Alexander aut Robin, Yves Marie aut Revillion, Françoise aut Triki, Magali Lacroix aut Seaume, Aline aut Salomon, Anne Vincent aut de Cremoux, Patricia aut Portefaix, Geneviève aut Xerri, Luc aut Vacher, Sophie aut Bièche, Ivan aut Penault-Llorca, Frédérique aut Enthalten in BMC cancer London : BioMed Central, 2001 13(2013), 1 vom: 22. Juli (DE-627)326643710 (DE-600)2041352-X 1471-2407 nnns volume:13 year:2013 number:1 day:22 month:07 https://dx.doi.org/10.1186/1471-2407-13-351 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2013 1 22 07
allfieldsSound	10.1186/1471-2407-13-351 doi (DE-627)SPR027647064 (SPR)1471-2407-13-351-e DE-627 ger DE-627 rakwb eng Jacquemier, Jocelyne verfasserin aut SISH/CISH or qPCR as alternative techniques to FISH for determination of HER2 amplification status on breast tumors core needle biopsies: a multicenter experience based on 840 cases 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Jacquemier et al.; licensee BioMed Central Ltd. 2013 Background Until now, FISH has been the gold standard technique to identify HER2 amplification status in ambiguous cases of breast cancer. Alternative techniques have been developed to increase the capacities of investigating HER2 amplification status. The aims of this multicenter study in a large series of breast cancer patients were to prospectively compare the level of performance of CISH, SISH, and qPCR alternative techniques on paraffin-embedded core biopsies with “gold standard FISH” for evaluation of HER2 amplification status. Methods This study was performed on 840 cases scored by immunohistochemistry (IHC): 0=317 (38%), 1+=183 (22%), 2+=109 (13%), 3+=231 (27%). Each of the 15 French centers participating in the study analyzed 56 breast carcinoma cases diagnosed on fixed paraffin-embedded core biopsies. HER2 amplification status was determined by commercially available FISH used as the reference technique with determination of the HER2/CEN17 ratio or HER2 copy number status. The alternative techniques performed on the same cases were commercially available SISH or CISH and a common qPCR method especially designed for the study including a set of 10 primer pairs: 2 for HER2 (exons 8 and 26), 5 to evaluate chromosome 17 polysomy TAOK1, UTP6, MRM1, MKS1, SSTR2 and 3 for diploidy control TSN, LAP3 and ADAMTS16. Results The concordance between IHC and FISH was 96% to 95% based on the HER2/CEN17 ratio (n=766) or HER2 copy number (n=840), respectively. The concordance of the alternative techniques with FISH was excellent: 97% and 98% for SISH (498 and 587 cases), 98% and 75% for CISH (108 and 204 cases) and 95% and 93% (699 and 773 cases) for qPCR based on the HER2/CEN17 ratio or HER2 copy number, respectively. Similarly, sensitivity ranged from 99% to 95% for SISH, 100% to 99% for CISH and 89% to 80% for qPCR. The concordance with FISH (ratio) in the 2+ cases was 89% for SISH, 100% for CISH and 93% for qPCR. Conclusion These alternative techniques showed an excellent concordance with FISH in core biopsies allowing their use in routine clinical practice. This newly designed qPCR on paraffin-embedded core biopsies deserves special attention, as it is reliable, easy to perform and less expensive than ISH tests. HER2 (dpeaa)DE-He213 FISH (dpeaa)DE-He213 SISH (dpeaa)DE-He213 CISH (dpeaa)DE-He213 qPCR (dpeaa)DE-He213 Multicenter analysis (dpeaa)DE-He213 Spyratos, Frédérique aut Esterni, Benjamin aut Mozziconacci, Marie-Joëlle aut Antoine, Martine aut Arnould, Laurent aut Lizard, Sarab aut Bertheau, Philippe aut Lehmann-Che, Jacqueline aut Fournier, Cécile Blanc aut Krieger, Sophie aut Bibeau, Frédéric aut Lamy, Pierre-Jean aut Chenard, Marie Pierre aut Legrain, Michèle aut Guinebretière, Jean-Marc aut Loussouarn, Delphine aut MacGrogan, Gaëtan aut Hostein, Isabelle aut Mathieu, Marie Christine aut Lacroix, Ludovic aut Valent, Alexander aut Robin, Yves Marie aut Revillion, Françoise aut Triki, Magali Lacroix aut Seaume, Aline aut Salomon, Anne Vincent aut de Cremoux, Patricia aut Portefaix, Geneviève aut Xerri, Luc aut Vacher, Sophie aut Bièche, Ivan aut Penault-Llorca, Frédérique aut Enthalten in BMC cancer London : BioMed Central, 2001 13(2013), 1 vom: 22. Juli (DE-627)326643710 (DE-600)2041352-X 1471-2407 nnns volume:13 year:2013 number:1 day:22 month:07 https://dx.doi.org/10.1186/1471-2407-13-351 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2013 1 22 07
language	English
source	Enthalten in BMC cancer 13(2013), 1 vom: 22. Juli volume:13 year:2013 number:1 day:22 month:07
sourceStr	Enthalten in BMC cancer 13(2013), 1 vom: 22. Juli volume:13 year:2013 number:1 day:22 month:07
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	HER2 FISH SISH CISH qPCR Multicenter analysis
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container_title	BMC cancer
authorswithroles_txt_mv	Jacquemier, Jocelyne @@aut@@ Spyratos, Frédérique @@aut@@ Esterni, Benjamin @@aut@@ Mozziconacci, Marie-Joëlle @@aut@@ Antoine, Martine @@aut@@ Arnould, Laurent @@aut@@ Lizard, Sarab @@aut@@ Bertheau, Philippe @@aut@@ Lehmann-Che, Jacqueline @@aut@@ Fournier, Cécile Blanc @@aut@@ Krieger, Sophie @@aut@@ Bibeau, Frédéric @@aut@@ Lamy, Pierre-Jean @@aut@@ Chenard, Marie Pierre @@aut@@ Legrain, Michèle @@aut@@ Guinebretière, Jean-Marc @@aut@@ Loussouarn, Delphine @@aut@@ MacGrogan, Gaëtan @@aut@@ Hostein, Isabelle @@aut@@ Mathieu, Marie Christine @@aut@@ Lacroix, Ludovic @@aut@@ Valent, Alexander @@aut@@ Robin, Yves Marie @@aut@@ Revillion, Françoise @@aut@@ Triki, Magali Lacroix @@aut@@ Seaume, Aline @@aut@@ Salomon, Anne Vincent @@aut@@ de Cremoux, Patricia @@aut@@ Portefaix, Geneviève @@aut@@ Xerri, Luc @@aut@@ Vacher, Sophie @@aut@@ Bièche, Ivan @@aut@@ Penault-Llorca, Frédérique @@aut@@
publishDateDaySort_date	2013-07-22T00:00:00Z
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author	Jacquemier, Jocelyne
spellingShingle	Jacquemier, Jocelyne misc HER2 misc FISH misc SISH misc CISH misc qPCR misc Multicenter analysis SISH/CISH or qPCR as alternative techniques to FISH for determination of HER2 amplification status on breast tumors core needle biopsies: a multicenter experience based on 840 cases
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topic_title	SISH/CISH or qPCR as alternative techniques to FISH for determination of HER2 amplification status on breast tumors core needle biopsies: a multicenter experience based on 840 cases HER2 (dpeaa)DE-He213 FISH (dpeaa)DE-He213 SISH (dpeaa)DE-He213 CISH (dpeaa)DE-He213 qPCR (dpeaa)DE-He213 Multicenter analysis (dpeaa)DE-He213
topic	misc HER2 misc FISH misc SISH misc CISH misc qPCR misc Multicenter analysis
topic_unstemmed	misc HER2 misc FISH misc SISH misc CISH misc qPCR misc Multicenter analysis
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title	SISH/CISH or qPCR as alternative techniques to FISH for determination of HER2 amplification status on breast tumors core needle biopsies: a multicenter experience based on 840 cases
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title_full	SISH/CISH or qPCR as alternative techniques to FISH for determination of HER2 amplification status on breast tumors core needle biopsies: a multicenter experience based on 840 cases
author_sort	Jacquemier, Jocelyne
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author_browse	Jacquemier, Jocelyne Spyratos, Frédérique Esterni, Benjamin Mozziconacci, Marie-Joëlle Antoine, Martine Arnould, Laurent Lizard, Sarab Bertheau, Philippe Lehmann-Che, Jacqueline Fournier, Cécile Blanc Krieger, Sophie Bibeau, Frédéric Lamy, Pierre-Jean Chenard, Marie Pierre Legrain, Michèle Guinebretière, Jean-Marc Loussouarn, Delphine MacGrogan, Gaëtan Hostein, Isabelle Mathieu, Marie Christine Lacroix, Ludovic Valent, Alexander Robin, Yves Marie Revillion, Françoise Triki, Magali Lacroix Seaume, Aline Salomon, Anne Vincent de Cremoux, Patricia Portefaix, Geneviève Xerri, Luc Vacher, Sophie Bièche, Ivan Penault-Llorca, Frédérique
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doi_str_mv	10.1186/1471-2407-13-351
title_sort	sish/cish or qpcr as alternative techniques to fish for determination of her2 amplification status on breast tumors core needle biopsies: a multicenter experience based on 840 cases
title_auth	SISH/CISH or qPCR as alternative techniques to FISH for determination of HER2 amplification status on breast tumors core needle biopsies: a multicenter experience based on 840 cases
abstract	Background Until now, FISH has been the gold standard technique to identify HER2 amplification status in ambiguous cases of breast cancer. Alternative techniques have been developed to increase the capacities of investigating HER2 amplification status. The aims of this multicenter study in a large series of breast cancer patients were to prospectively compare the level of performance of CISH, SISH, and qPCR alternative techniques on paraffin-embedded core biopsies with “gold standard FISH” for evaluation of HER2 amplification status. Methods This study was performed on 840 cases scored by immunohistochemistry (IHC): 0=317 (38%), 1+=183 (22%), 2+=109 (13%), 3+=231 (27%). Each of the 15 French centers participating in the study analyzed 56 breast carcinoma cases diagnosed on fixed paraffin-embedded core biopsies. HER2 amplification status was determined by commercially available FISH used as the reference technique with determination of the HER2/CEN17 ratio or HER2 copy number status. The alternative techniques performed on the same cases were commercially available SISH or CISH and a common qPCR method especially designed for the study including a set of 10 primer pairs: 2 for HER2 (exons 8 and 26), 5 to evaluate chromosome 17 polysomy TAOK1, UTP6, MRM1, MKS1, SSTR2 and 3 for diploidy control TSN, LAP3 and ADAMTS16. Results The concordance between IHC and FISH was 96% to 95% based on the HER2/CEN17 ratio (n=766) or HER2 copy number (n=840), respectively. The concordance of the alternative techniques with FISH was excellent: 97% and 98% for SISH (498 and 587 cases), 98% and 75% for CISH (108 and 204 cases) and 95% and 93% (699 and 773 cases) for qPCR based on the HER2/CEN17 ratio or HER2 copy number, respectively. Similarly, sensitivity ranged from 99% to 95% for SISH, 100% to 99% for CISH and 89% to 80% for qPCR. The concordance with FISH (ratio) in the 2+ cases was 89% for SISH, 100% for CISH and 93% for qPCR. Conclusion These alternative techniques showed an excellent concordance with FISH in core biopsies allowing their use in routine clinical practice. This newly designed qPCR on paraffin-embedded core biopsies deserves special attention, as it is reliable, easy to perform and less expensive than ISH tests. © Jacquemier et al.; licensee BioMed Central Ltd. 2013
abstractGer	Background Until now, FISH has been the gold standard technique to identify HER2 amplification status in ambiguous cases of breast cancer. Alternative techniques have been developed to increase the capacities of investigating HER2 amplification status. The aims of this multicenter study in a large series of breast cancer patients were to prospectively compare the level of performance of CISH, SISH, and qPCR alternative techniques on paraffin-embedded core biopsies with “gold standard FISH” for evaluation of HER2 amplification status. Methods This study was performed on 840 cases scored by immunohistochemistry (IHC): 0=317 (38%), 1+=183 (22%), 2+=109 (13%), 3+=231 (27%). Each of the 15 French centers participating in the study analyzed 56 breast carcinoma cases diagnosed on fixed paraffin-embedded core biopsies. HER2 amplification status was determined by commercially available FISH used as the reference technique with determination of the HER2/CEN17 ratio or HER2 copy number status. The alternative techniques performed on the same cases were commercially available SISH or CISH and a common qPCR method especially designed for the study including a set of 10 primer pairs: 2 for HER2 (exons 8 and 26), 5 to evaluate chromosome 17 polysomy TAOK1, UTP6, MRM1, MKS1, SSTR2 and 3 for diploidy control TSN, LAP3 and ADAMTS16. Results The concordance between IHC and FISH was 96% to 95% based on the HER2/CEN17 ratio (n=766) or HER2 copy number (n=840), respectively. The concordance of the alternative techniques with FISH was excellent: 97% and 98% for SISH (498 and 587 cases), 98% and 75% for CISH (108 and 204 cases) and 95% and 93% (699 and 773 cases) for qPCR based on the HER2/CEN17 ratio or HER2 copy number, respectively. Similarly, sensitivity ranged from 99% to 95% for SISH, 100% to 99% for CISH and 89% to 80% for qPCR. The concordance with FISH (ratio) in the 2+ cases was 89% for SISH, 100% for CISH and 93% for qPCR. Conclusion These alternative techniques showed an excellent concordance with FISH in core biopsies allowing their use in routine clinical practice. This newly designed qPCR on paraffin-embedded core biopsies deserves special attention, as it is reliable, easy to perform and less expensive than ISH tests. © Jacquemier et al.; licensee BioMed Central Ltd. 2013
abstract_unstemmed	Background Until now, FISH has been the gold standard technique to identify HER2 amplification status in ambiguous cases of breast cancer. Alternative techniques have been developed to increase the capacities of investigating HER2 amplification status. The aims of this multicenter study in a large series of breast cancer patients were to prospectively compare the level of performance of CISH, SISH, and qPCR alternative techniques on paraffin-embedded core biopsies with “gold standard FISH” for evaluation of HER2 amplification status. Methods This study was performed on 840 cases scored by immunohistochemistry (IHC): 0=317 (38%), 1+=183 (22%), 2+=109 (13%), 3+=231 (27%). Each of the 15 French centers participating in the study analyzed 56 breast carcinoma cases diagnosed on fixed paraffin-embedded core biopsies. HER2 amplification status was determined by commercially available FISH used as the reference technique with determination of the HER2/CEN17 ratio or HER2 copy number status. The alternative techniques performed on the same cases were commercially available SISH or CISH and a common qPCR method especially designed for the study including a set of 10 primer pairs: 2 for HER2 (exons 8 and 26), 5 to evaluate chromosome 17 polysomy TAOK1, UTP6, MRM1, MKS1, SSTR2 and 3 for diploidy control TSN, LAP3 and ADAMTS16. Results The concordance between IHC and FISH was 96% to 95% based on the HER2/CEN17 ratio (n=766) or HER2 copy number (n=840), respectively. The concordance of the alternative techniques with FISH was excellent: 97% and 98% for SISH (498 and 587 cases), 98% and 75% for CISH (108 and 204 cases) and 95% and 93% (699 and 773 cases) for qPCR based on the HER2/CEN17 ratio or HER2 copy number, respectively. Similarly, sensitivity ranged from 99% to 95% for SISH, 100% to 99% for CISH and 89% to 80% for qPCR. The concordance with FISH (ratio) in the 2+ cases was 89% for SISH, 100% for CISH and 93% for qPCR. Conclusion These alternative techniques showed an excellent concordance with FISH in core biopsies allowing their use in routine clinical practice. This newly designed qPCR on paraffin-embedded core biopsies deserves special attention, as it is reliable, easy to perform and less expensive than ISH tests. © Jacquemier et al.; licensee BioMed Central Ltd. 2013
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title_short	SISH/CISH or qPCR as alternative techniques to FISH for determination of HER2 amplification status on breast tumors core needle biopsies: a multicenter experience based on 840 cases
url	https://dx.doi.org/10.1186/1471-2407-13-351
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author2	Spyratos, Frédérique Esterni, Benjamin Mozziconacci, Marie-Joëlle Antoine, Martine Arnould, Laurent Lizard, Sarab Bertheau, Philippe Lehmann-Che, Jacqueline Fournier, Cécile Blanc Krieger, Sophie Bibeau, Frédéric Lamy, Pierre-Jean Chenard, Marie Pierre Legrain, Michèle Guinebretière, Jean-Marc Loussouarn, Delphine MacGrogan, Gaëtan Hostein, Isabelle Mathieu, Marie Christine Lacroix, Ludovic Valent, Alexander Robin, Yves Marie Revillion, Françoise Triki, Magali Lacroix Seaume, Aline Salomon, Anne Vincent de Cremoux, Patricia Portefaix, Geneviève Xerri, Luc Vacher, Sophie Bièche, Ivan Penault-Llorca, Frédérique
author2Str	Spyratos, Frédérique Esterni, Benjamin Mozziconacci, Marie-Joëlle Antoine, Martine Arnould, Laurent Lizard, Sarab Bertheau, Philippe Lehmann-Che, Jacqueline Fournier, Cécile Blanc Krieger, Sophie Bibeau, Frédéric Lamy, Pierre-Jean Chenard, Marie Pierre Legrain, Michèle Guinebretière, Jean-Marc Loussouarn, Delphine MacGrogan, Gaëtan Hostein, Isabelle Mathieu, Marie Christine Lacroix, Ludovic Valent, Alexander Robin, Yves Marie Revillion, Françoise Triki, Magali Lacroix Seaume, Aline Salomon, Anne Vincent de Cremoux, Patricia Portefaix, Geneviève Xerri, Luc Vacher, Sophie Bièche, Ivan Penault-Llorca, Frédérique
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doi_str	10.1186/1471-2407-13-351
up_date	2024-07-03T14:12:30.267Z
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SISH/CISH or qPCR as alternative techniques to FISH for determination of HER2 amplification status on breast tumors core needle biopsies: a multicenter experience based on 840 cases

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