Improvement of the quality of BRAF testing in melanomas with nationwide external quality assessment, for the BRAF EQA group

Background Knowledge about tumour gene mutation status is essential for the treatment of increasing numbers of cancer patients, and testing quality has a major impact on treatment response and cost. In 2012, 4,629 tests for BRAF p.V600 were performed in France, in patients with melanomas. Methods Tw...
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Autor*in:	Emile, Jean-François [verfasserIn] Tisserand, Julie Bergougnoux, Loic Nowak, Frédérique Faucher, Gladwys Surel, Sylvie Lamy, Aude Lecorre, Delphine Helias-Rodzewicz, Zofia Hofman, Paul Sabourin, Jean-Christophe Laurent-Puig, Pierre

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2013

Schlagwörter:	BRAF Mutation Vemurafenib BRAF Inhibitor External Quality Assessment FFPE Sample

Anmerkung:	© Emile et al.; licensee BioMed Central Ltd. 2013

Übergeordnetes Werk:	Enthalten in: BMC cancer - London : BioMed Central, 2001, 13(2013), 1 vom: 11. Okt.
Übergeordnetes Werk:	volume:13 ; year:2013 ; number:1 ; day:11 ; month:10

Links:	Volltext

DOI / URN:	10.1186/1471-2407-13-472

Katalog-ID:	SPR027648230

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520			\|a Background Knowledge about tumour gene mutation status is essential for the treatment of increasing numbers of cancer patients, and testing quality has a major impact on treatment response and cost. In 2012, 4,629 tests for BRAF p.V600 were performed in France, in patients with melanomas. Methods Two batches of unstained melanoma sections were sent, in May and November 2012, to the 46 laboratories supported by the French National Institute of Cancer (INCa). An external quality assessment (EQA) evaluated mutation status, response times and compliance with INCa recommendations. Results All the French laboratories involved in testing participated in the EQA. Fourteen different methods were used to detect BRAF mutations, most consisting of combinations of in-house techniques. False responses were noted in 25/520 cases (4.8%), 11 of which concerned confusion between p.V600E and p.V600K. Thus, 2.7% of responses would have led to inappropriate treatment. Within six months, mean response times decreased from 22 to 12 days (P<0.001), and the percentage of samples evaluated by a pathologist for tumour cell content increased, from 75.2% to 96.9% (P<0.001). Conclusion Despite the use of non-certified methods, the false response rate was low. Nationwide EQA can improve the quality of molecular pathology tests on tumours.
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allfields	10.1186/1471-2407-13-472 doi (DE-627)SPR027648230 (SPR)1471-2407-13-472-e DE-627 ger DE-627 rakwb eng Emile, Jean-François verfasserin aut Improvement of the quality of BRAF testing in melanomas with nationwide external quality assessment, for the BRAF EQA group 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Emile et al.; licensee BioMed Central Ltd. 2013 Background Knowledge about tumour gene mutation status is essential for the treatment of increasing numbers of cancer patients, and testing quality has a major impact on treatment response and cost. In 2012, 4,629 tests for BRAF p.V600 were performed in France, in patients with melanomas. Methods Two batches of unstained melanoma sections were sent, in May and November 2012, to the 46 laboratories supported by the French National Institute of Cancer (INCa). An external quality assessment (EQA) evaluated mutation status, response times and compliance with INCa recommendations. Results All the French laboratories involved in testing participated in the EQA. Fourteen different methods were used to detect BRAF mutations, most consisting of combinations of in-house techniques. False responses were noted in 25/520 cases (4.8%), 11 of which concerned confusion between p.V600E and p.V600K. Thus, 2.7% of responses would have led to inappropriate treatment. Within six months, mean response times decreased from 22 to 12 days (P<0.001), and the percentage of samples evaluated by a pathologist for tumour cell content increased, from 75.2% to 96.9% (P<0.001). Conclusion Despite the use of non-certified methods, the false response rate was low. Nationwide EQA can improve the quality of molecular pathology tests on tumours. BRAF Mutation (dpeaa)DE-He213 Vemurafenib (dpeaa)DE-He213 BRAF Inhibitor (dpeaa)DE-He213 External Quality Assessment (dpeaa)DE-He213 FFPE Sample (dpeaa)DE-He213 Tisserand, Julie aut Bergougnoux, Loic aut Nowak, Frédérique aut Faucher, Gladwys aut Surel, Sylvie aut Lamy, Aude aut Lecorre, Delphine aut Helias-Rodzewicz, Zofia aut Hofman, Paul aut Sabourin, Jean-Christophe aut Laurent-Puig, Pierre aut Enthalten in BMC cancer London : BioMed Central, 2001 13(2013), 1 vom: 11. Okt. (DE-627)326643710 (DE-600)2041352-X 1471-2407 nnns volume:13 year:2013 number:1 day:11 month:10 https://dx.doi.org/10.1186/1471-2407-13-472 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2013 1 11 10
spelling	10.1186/1471-2407-13-472 doi (DE-627)SPR027648230 (SPR)1471-2407-13-472-e DE-627 ger DE-627 rakwb eng Emile, Jean-François verfasserin aut Improvement of the quality of BRAF testing in melanomas with nationwide external quality assessment, for the BRAF EQA group 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Emile et al.; licensee BioMed Central Ltd. 2013 Background Knowledge about tumour gene mutation status is essential for the treatment of increasing numbers of cancer patients, and testing quality has a major impact on treatment response and cost. In 2012, 4,629 tests for BRAF p.V600 were performed in France, in patients with melanomas. Methods Two batches of unstained melanoma sections were sent, in May and November 2012, to the 46 laboratories supported by the French National Institute of Cancer (INCa). An external quality assessment (EQA) evaluated mutation status, response times and compliance with INCa recommendations. Results All the French laboratories involved in testing participated in the EQA. Fourteen different methods were used to detect BRAF mutations, most consisting of combinations of in-house techniques. False responses were noted in 25/520 cases (4.8%), 11 of which concerned confusion between p.V600E and p.V600K. Thus, 2.7% of responses would have led to inappropriate treatment. Within six months, mean response times decreased from 22 to 12 days (P<0.001), and the percentage of samples evaluated by a pathologist for tumour cell content increased, from 75.2% to 96.9% (P<0.001). Conclusion Despite the use of non-certified methods, the false response rate was low. Nationwide EQA can improve the quality of molecular pathology tests on tumours. BRAF Mutation (dpeaa)DE-He213 Vemurafenib (dpeaa)DE-He213 BRAF Inhibitor (dpeaa)DE-He213 External Quality Assessment (dpeaa)DE-He213 FFPE Sample (dpeaa)DE-He213 Tisserand, Julie aut Bergougnoux, Loic aut Nowak, Frédérique aut Faucher, Gladwys aut Surel, Sylvie aut Lamy, Aude aut Lecorre, Delphine aut Helias-Rodzewicz, Zofia aut Hofman, Paul aut Sabourin, Jean-Christophe aut Laurent-Puig, Pierre aut Enthalten in BMC cancer London : BioMed Central, 2001 13(2013), 1 vom: 11. Okt. (DE-627)326643710 (DE-600)2041352-X 1471-2407 nnns volume:13 year:2013 number:1 day:11 month:10 https://dx.doi.org/10.1186/1471-2407-13-472 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2013 1 11 10
allfields_unstemmed	10.1186/1471-2407-13-472 doi (DE-627)SPR027648230 (SPR)1471-2407-13-472-e DE-627 ger DE-627 rakwb eng Emile, Jean-François verfasserin aut Improvement of the quality of BRAF testing in melanomas with nationwide external quality assessment, for the BRAF EQA group 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Emile et al.; licensee BioMed Central Ltd. 2013 Background Knowledge about tumour gene mutation status is essential for the treatment of increasing numbers of cancer patients, and testing quality has a major impact on treatment response and cost. In 2012, 4,629 tests for BRAF p.V600 were performed in France, in patients with melanomas. Methods Two batches of unstained melanoma sections were sent, in May and November 2012, to the 46 laboratories supported by the French National Institute of Cancer (INCa). An external quality assessment (EQA) evaluated mutation status, response times and compliance with INCa recommendations. Results All the French laboratories involved in testing participated in the EQA. Fourteen different methods were used to detect BRAF mutations, most consisting of combinations of in-house techniques. False responses were noted in 25/520 cases (4.8%), 11 of which concerned confusion between p.V600E and p.V600K. Thus, 2.7% of responses would have led to inappropriate treatment. Within six months, mean response times decreased from 22 to 12 days (P<0.001), and the percentage of samples evaluated by a pathologist for tumour cell content increased, from 75.2% to 96.9% (P<0.001). Conclusion Despite the use of non-certified methods, the false response rate was low. Nationwide EQA can improve the quality of molecular pathology tests on tumours. BRAF Mutation (dpeaa)DE-He213 Vemurafenib (dpeaa)DE-He213 BRAF Inhibitor (dpeaa)DE-He213 External Quality Assessment (dpeaa)DE-He213 FFPE Sample (dpeaa)DE-He213 Tisserand, Julie aut Bergougnoux, Loic aut Nowak, Frédérique aut Faucher, Gladwys aut Surel, Sylvie aut Lamy, Aude aut Lecorre, Delphine aut Helias-Rodzewicz, Zofia aut Hofman, Paul aut Sabourin, Jean-Christophe aut Laurent-Puig, Pierre aut Enthalten in BMC cancer London : BioMed Central, 2001 13(2013), 1 vom: 11. Okt. (DE-627)326643710 (DE-600)2041352-X 1471-2407 nnns volume:13 year:2013 number:1 day:11 month:10 https://dx.doi.org/10.1186/1471-2407-13-472 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2013 1 11 10
allfieldsGer	10.1186/1471-2407-13-472 doi (DE-627)SPR027648230 (SPR)1471-2407-13-472-e DE-627 ger DE-627 rakwb eng Emile, Jean-François verfasserin aut Improvement of the quality of BRAF testing in melanomas with nationwide external quality assessment, for the BRAF EQA group 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Emile et al.; licensee BioMed Central Ltd. 2013 Background Knowledge about tumour gene mutation status is essential for the treatment of increasing numbers of cancer patients, and testing quality has a major impact on treatment response and cost. In 2012, 4,629 tests for BRAF p.V600 were performed in France, in patients with melanomas. Methods Two batches of unstained melanoma sections were sent, in May and November 2012, to the 46 laboratories supported by the French National Institute of Cancer (INCa). An external quality assessment (EQA) evaluated mutation status, response times and compliance with INCa recommendations. Results All the French laboratories involved in testing participated in the EQA. Fourteen different methods were used to detect BRAF mutations, most consisting of combinations of in-house techniques. False responses were noted in 25/520 cases (4.8%), 11 of which concerned confusion between p.V600E and p.V600K. Thus, 2.7% of responses would have led to inappropriate treatment. Within six months, mean response times decreased from 22 to 12 days (P<0.001), and the percentage of samples evaluated by a pathologist for tumour cell content increased, from 75.2% to 96.9% (P<0.001). Conclusion Despite the use of non-certified methods, the false response rate was low. Nationwide EQA can improve the quality of molecular pathology tests on tumours. BRAF Mutation (dpeaa)DE-He213 Vemurafenib (dpeaa)DE-He213 BRAF Inhibitor (dpeaa)DE-He213 External Quality Assessment (dpeaa)DE-He213 FFPE Sample (dpeaa)DE-He213 Tisserand, Julie aut Bergougnoux, Loic aut Nowak, Frédérique aut Faucher, Gladwys aut Surel, Sylvie aut Lamy, Aude aut Lecorre, Delphine aut Helias-Rodzewicz, Zofia aut Hofman, Paul aut Sabourin, Jean-Christophe aut Laurent-Puig, Pierre aut Enthalten in BMC cancer London : BioMed Central, 2001 13(2013), 1 vom: 11. Okt. (DE-627)326643710 (DE-600)2041352-X 1471-2407 nnns volume:13 year:2013 number:1 day:11 month:10 https://dx.doi.org/10.1186/1471-2407-13-472 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2013 1 11 10
allfieldsSound	10.1186/1471-2407-13-472 doi (DE-627)SPR027648230 (SPR)1471-2407-13-472-e DE-627 ger DE-627 rakwb eng Emile, Jean-François verfasserin aut Improvement of the quality of BRAF testing in melanomas with nationwide external quality assessment, for the BRAF EQA group 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Emile et al.; licensee BioMed Central Ltd. 2013 Background Knowledge about tumour gene mutation status is essential for the treatment of increasing numbers of cancer patients, and testing quality has a major impact on treatment response and cost. In 2012, 4,629 tests for BRAF p.V600 were performed in France, in patients with melanomas. Methods Two batches of unstained melanoma sections were sent, in May and November 2012, to the 46 laboratories supported by the French National Institute of Cancer (INCa). An external quality assessment (EQA) evaluated mutation status, response times and compliance with INCa recommendations. Results All the French laboratories involved in testing participated in the EQA. Fourteen different methods were used to detect BRAF mutations, most consisting of combinations of in-house techniques. False responses were noted in 25/520 cases (4.8%), 11 of which concerned confusion between p.V600E and p.V600K. Thus, 2.7% of responses would have led to inappropriate treatment. Within six months, mean response times decreased from 22 to 12 days (P<0.001), and the percentage of samples evaluated by a pathologist for tumour cell content increased, from 75.2% to 96.9% (P<0.001). Conclusion Despite the use of non-certified methods, the false response rate was low. Nationwide EQA can improve the quality of molecular pathology tests on tumours. BRAF Mutation (dpeaa)DE-He213 Vemurafenib (dpeaa)DE-He213 BRAF Inhibitor (dpeaa)DE-He213 External Quality Assessment (dpeaa)DE-He213 FFPE Sample (dpeaa)DE-He213 Tisserand, Julie aut Bergougnoux, Loic aut Nowak, Frédérique aut Faucher, Gladwys aut Surel, Sylvie aut Lamy, Aude aut Lecorre, Delphine aut Helias-Rodzewicz, Zofia aut Hofman, Paul aut Sabourin, Jean-Christophe aut Laurent-Puig, Pierre aut Enthalten in BMC cancer London : BioMed Central, 2001 13(2013), 1 vom: 11. Okt. (DE-627)326643710 (DE-600)2041352-X 1471-2407 nnns volume:13 year:2013 number:1 day:11 month:10 https://dx.doi.org/10.1186/1471-2407-13-472 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2013 1 11 10
language	English
source	Enthalten in BMC cancer 13(2013), 1 vom: 11. Okt. volume:13 year:2013 number:1 day:11 month:10
sourceStr	Enthalten in BMC cancer 13(2013), 1 vom: 11. Okt. volume:13 year:2013 number:1 day:11 month:10
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	BRAF Mutation Vemurafenib BRAF Inhibitor External Quality Assessment FFPE Sample
isfreeaccess_bool	true
container_title	BMC cancer
authorswithroles_txt_mv	Emile, Jean-François @@aut@@ Tisserand, Julie @@aut@@ Bergougnoux, Loic @@aut@@ Nowak, Frédérique @@aut@@ Faucher, Gladwys @@aut@@ Surel, Sylvie @@aut@@ Lamy, Aude @@aut@@ Lecorre, Delphine @@aut@@ Helias-Rodzewicz, Zofia @@aut@@ Hofman, Paul @@aut@@ Sabourin, Jean-Christophe @@aut@@ Laurent-Puig, Pierre @@aut@@
publishDateDaySort_date	2013-10-11T00:00:00Z
hierarchy_top_id	326643710
id	SPR027648230
language_de	englisch
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In 2012, 4,629 tests for BRAF p.V600 were performed in France, in patients with melanomas. Methods Two batches of unstained melanoma sections were sent, in May and November 2012, to the 46 laboratories supported by the French National Institute of Cancer (INCa). An external quality assessment (EQA) evaluated mutation status, response times and compliance with INCa recommendations. Results All the French laboratories involved in testing participated in the EQA. Fourteen different methods were used to detect BRAF mutations, most consisting of combinations of in-house techniques. False responses were noted in 25/520 cases (4.8%), 11 of which concerned confusion between p.V600E and p.V600K. Thus, 2.7% of responses would have led to inappropriate treatment. Within six months, mean response times decreased from 22 to 12 days (P<0.001), and the percentage of samples evaluated by a pathologist for tumour cell content increased, from 75.2% to 96.9% (P<0.001). 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author	Emile, Jean-François
spellingShingle	Emile, Jean-François misc BRAF Mutation misc Vemurafenib misc BRAF Inhibitor misc External Quality Assessment misc FFPE Sample Improvement of the quality of BRAF testing in melanomas with nationwide external quality assessment, for the BRAF EQA group
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topic_title	Improvement of the quality of BRAF testing in melanomas with nationwide external quality assessment, for the BRAF EQA group BRAF Mutation (dpeaa)DE-He213 Vemurafenib (dpeaa)DE-He213 BRAF Inhibitor (dpeaa)DE-He213 External Quality Assessment (dpeaa)DE-He213 FFPE Sample (dpeaa)DE-He213
topic	misc BRAF Mutation misc Vemurafenib misc BRAF Inhibitor misc External Quality Assessment misc FFPE Sample
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title	Improvement of the quality of BRAF testing in melanomas with nationwide external quality assessment, for the BRAF EQA group
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title_full	Improvement of the quality of BRAF testing in melanomas with nationwide external quality assessment, for the BRAF EQA group
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author_browse	Emile, Jean-François Tisserand, Julie Bergougnoux, Loic Nowak, Frédérique Faucher, Gladwys Surel, Sylvie Lamy, Aude Lecorre, Delphine Helias-Rodzewicz, Zofia Hofman, Paul Sabourin, Jean-Christophe Laurent-Puig, Pierre
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author-letter	Emile, Jean-François
doi_str_mv	10.1186/1471-2407-13-472
title_sort	improvement of the quality of braf testing in melanomas with nationwide external quality assessment, for the braf eqa group
title_auth	Improvement of the quality of BRAF testing in melanomas with nationwide external quality assessment, for the BRAF EQA group
abstract	Background Knowledge about tumour gene mutation status is essential for the treatment of increasing numbers of cancer patients, and testing quality has a major impact on treatment response and cost. In 2012, 4,629 tests for BRAF p.V600 were performed in France, in patients with melanomas. Methods Two batches of unstained melanoma sections were sent, in May and November 2012, to the 46 laboratories supported by the French National Institute of Cancer (INCa). An external quality assessment (EQA) evaluated mutation status, response times and compliance with INCa recommendations. Results All the French laboratories involved in testing participated in the EQA. Fourteen different methods were used to detect BRAF mutations, most consisting of combinations of in-house techniques. False responses were noted in 25/520 cases (4.8%), 11 of which concerned confusion between p.V600E and p.V600K. Thus, 2.7% of responses would have led to inappropriate treatment. Within six months, mean response times decreased from 22 to 12 days (P<0.001), and the percentage of samples evaluated by a pathologist for tumour cell content increased, from 75.2% to 96.9% (P<0.001). Conclusion Despite the use of non-certified methods, the false response rate was low. Nationwide EQA can improve the quality of molecular pathology tests on tumours. © Emile et al.; licensee BioMed Central Ltd. 2013
abstractGer	Background Knowledge about tumour gene mutation status is essential for the treatment of increasing numbers of cancer patients, and testing quality has a major impact on treatment response and cost. In 2012, 4,629 tests for BRAF p.V600 were performed in France, in patients with melanomas. Methods Two batches of unstained melanoma sections were sent, in May and November 2012, to the 46 laboratories supported by the French National Institute of Cancer (INCa). An external quality assessment (EQA) evaluated mutation status, response times and compliance with INCa recommendations. Results All the French laboratories involved in testing participated in the EQA. Fourteen different methods were used to detect BRAF mutations, most consisting of combinations of in-house techniques. False responses were noted in 25/520 cases (4.8%), 11 of which concerned confusion between p.V600E and p.V600K. Thus, 2.7% of responses would have led to inappropriate treatment. Within six months, mean response times decreased from 22 to 12 days (P<0.001), and the percentage of samples evaluated by a pathologist for tumour cell content increased, from 75.2% to 96.9% (P<0.001). Conclusion Despite the use of non-certified methods, the false response rate was low. Nationwide EQA can improve the quality of molecular pathology tests on tumours. © Emile et al.; licensee BioMed Central Ltd. 2013
abstract_unstemmed	Background Knowledge about tumour gene mutation status is essential for the treatment of increasing numbers of cancer patients, and testing quality has a major impact on treatment response and cost. In 2012, 4,629 tests for BRAF p.V600 were performed in France, in patients with melanomas. Methods Two batches of unstained melanoma sections were sent, in May and November 2012, to the 46 laboratories supported by the French National Institute of Cancer (INCa). An external quality assessment (EQA) evaluated mutation status, response times and compliance with INCa recommendations. Results All the French laboratories involved in testing participated in the EQA. Fourteen different methods were used to detect BRAF mutations, most consisting of combinations of in-house techniques. False responses were noted in 25/520 cases (4.8%), 11 of which concerned confusion between p.V600E and p.V600K. Thus, 2.7% of responses would have led to inappropriate treatment. Within six months, mean response times decreased from 22 to 12 days (P<0.001), and the percentage of samples evaluated by a pathologist for tumour cell content increased, from 75.2% to 96.9% (P<0.001). Conclusion Despite the use of non-certified methods, the false response rate was low. Nationwide EQA can improve the quality of molecular pathology tests on tumours. © Emile et al.; licensee BioMed Central Ltd. 2013
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title_short	Improvement of the quality of BRAF testing in melanomas with nationwide external quality assessment, for the BRAF EQA group
url	https://dx.doi.org/10.1186/1471-2407-13-472
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author2	Tisserand, Julie Bergougnoux, Loic Nowak, Frédérique Faucher, Gladwys Surel, Sylvie Lamy, Aude Lecorre, Delphine Helias-Rodzewicz, Zofia Hofman, Paul Sabourin, Jean-Christophe Laurent-Puig, Pierre
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Improvement of the quality of BRAF testing in melanomas with nationwide external quality assessment, for the BRAF EQA group

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