Prognostic and clinical impact of PIK3CA mutation in gastric cancer: pyrosequencing technology and literature review

Background Phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha (PIK3CA) mutations that activate the PI3K/AKT signaling pathway have been observed in several types of carcinoma and have been associated with patient prognosis. However, the significance of PIK3CA mutations in gastri...
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Autor*in:	Harada, Kazuto [verfasserIn] Baba, Yoshifumi Shigaki, Hironobu Ishimoto, Takatsugu Miyake, Keisuke Kosumi, Keisuke Tokunaga, Ryuma Izumi, Daisuke Ohuchi, Mayuko Nakamura, Kenichi Kiyozumi, Yuki Kurashige, Junji Iwatsuki, Masaaki Miyamoto, Yuji Sakamoto, Yasuo Yoshida, Naoya Watanabe, Masayuki Baba, Hideo

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2016

Schlagwörter:	PIK3CA mutation Gastric cancer Pyrosequencing Prognosis

Anmerkung:	© The Author(s). 2016

Übergeordnetes Werk:	Enthalten in: BMC cancer - London : BioMed Central, 2001, 16(2016), 1 vom: 07. Juli
Übergeordnetes Werk:	volume:16 ; year:2016 ; number:1 ; day:07 ; month:07

Links:	Volltext

DOI / URN:	10.1186/s12885-016-2422-y

Katalog-ID:	SPR027676935

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520			\|a Background Phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha (PIK3CA) mutations that activate the PI3K/AKT signaling pathway have been observed in several types of carcinoma and have been associated with patient prognosis. However, the significance of PIK3CA mutations in gastric cancer remains unclear. This retrospective study investigated the relationship between PIK3CA mutations and clinical outcomes in patients with gastric cancer. Additionally, we reviewed the rate of PIK3CA mutations in gastric cancer and the association between PIK3CA mutations and prognosis in human cancers. Methods The study included 208 patients with gastric cancer who underwent surgical resection at Kumamoto University Hospital, Japan, between January 2001 and August 2010. Mutations in PIK3CA exons 9 and 20 were quantified by pyrosequencing assays. Results PIK3CA mutations were detected in 25 (12 %) of the 208 patients. Ten patients had c.1634A > G (p.E545G), 10 had c.1624G > A (p.E542K), 13 had c.1633G > A (p.E545K), nine had c.3139C > T (p.H1047R), and 1 had c.3140A > G (p.H1047Y) mutations. PIK3CA mutations were not significantly associated with any clinical, epidemiologic, or pathologic characteristic. Kaplan–Meier analysis showed no significant differences in disease-free survival (log rank P = 0.84) and overall survival (log rank P = 0.74) between patients with and without PIK3CA mutations. Conclusions Mutations in PIK3CA did not correlate with prognosis in patients with gastric cancer, providing additional evidence for the lack of relationship between the two.
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allfields	10.1186/s12885-016-2422-y doi (DE-627)SPR027676935 (SPR)s12885-016-2422-y-e DE-627 ger DE-627 rakwb eng Harada, Kazuto verfasserin aut Prognostic and clinical impact of PIK3CA mutation in gastric cancer: pyrosequencing technology and literature review 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2016 Background Phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha (PIK3CA) mutations that activate the PI3K/AKT signaling pathway have been observed in several types of carcinoma and have been associated with patient prognosis. However, the significance of PIK3CA mutations in gastric cancer remains unclear. This retrospective study investigated the relationship between PIK3CA mutations and clinical outcomes in patients with gastric cancer. Additionally, we reviewed the rate of PIK3CA mutations in gastric cancer and the association between PIK3CA mutations and prognosis in human cancers. Methods The study included 208 patients with gastric cancer who underwent surgical resection at Kumamoto University Hospital, Japan, between January 2001 and August 2010. Mutations in PIK3CA exons 9 and 20 were quantified by pyrosequencing assays. Results PIK3CA mutations were detected in 25 (12 %) of the 208 patients. Ten patients had c.1634A > G (p.E545G), 10 had c.1624G > A (p.E542K), 13 had c.1633G > A (p.E545K), nine had c.3139C > T (p.H1047R), and 1 had c.3140A > G (p.H1047Y) mutations. PIK3CA mutations were not significantly associated with any clinical, epidemiologic, or pathologic characteristic. Kaplan–Meier analysis showed no significant differences in disease-free survival (log rank P = 0.84) and overall survival (log rank P = 0.74) between patients with and without PIK3CA mutations. Conclusions Mutations in PIK3CA did not correlate with prognosis in patients with gastric cancer, providing additional evidence for the lack of relationship between the two. PIK3CA mutation (dpeaa)DE-He213 Gastric cancer (dpeaa)DE-He213 Pyrosequencing (dpeaa)DE-He213 Prognosis (dpeaa)DE-He213 Baba, Yoshifumi aut Shigaki, Hironobu aut Ishimoto, Takatsugu aut Miyake, Keisuke aut Kosumi, Keisuke aut Tokunaga, Ryuma aut Izumi, Daisuke aut Ohuchi, Mayuko aut Nakamura, Kenichi aut Kiyozumi, Yuki aut Kurashige, Junji aut Iwatsuki, Masaaki aut Miyamoto, Yuji aut Sakamoto, Yasuo aut Yoshida, Naoya aut Watanabe, Masayuki aut Baba, Hideo aut Enthalten in BMC cancer London : BioMed Central, 2001 16(2016), 1 vom: 07. Juli (DE-627)326643710 (DE-600)2041352-X 1471-2407 nnns volume:16 year:2016 number:1 day:07 month:07 https://dx.doi.org/10.1186/s12885-016-2422-y kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 16 2016 1 07 07
spelling	10.1186/s12885-016-2422-y doi (DE-627)SPR027676935 (SPR)s12885-016-2422-y-e DE-627 ger DE-627 rakwb eng Harada, Kazuto verfasserin aut Prognostic and clinical impact of PIK3CA mutation in gastric cancer: pyrosequencing technology and literature review 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2016 Background Phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha (PIK3CA) mutations that activate the PI3K/AKT signaling pathway have been observed in several types of carcinoma and have been associated with patient prognosis. However, the significance of PIK3CA mutations in gastric cancer remains unclear. This retrospective study investigated the relationship between PIK3CA mutations and clinical outcomes in patients with gastric cancer. Additionally, we reviewed the rate of PIK3CA mutations in gastric cancer and the association between PIK3CA mutations and prognosis in human cancers. Methods The study included 208 patients with gastric cancer who underwent surgical resection at Kumamoto University Hospital, Japan, between January 2001 and August 2010. Mutations in PIK3CA exons 9 and 20 were quantified by pyrosequencing assays. Results PIK3CA mutations were detected in 25 (12 %) of the 208 patients. Ten patients had c.1634A > G (p.E545G), 10 had c.1624G > A (p.E542K), 13 had c.1633G > A (p.E545K), nine had c.3139C > T (p.H1047R), and 1 had c.3140A > G (p.H1047Y) mutations. PIK3CA mutations were not significantly associated with any clinical, epidemiologic, or pathologic characteristic. Kaplan–Meier analysis showed no significant differences in disease-free survival (log rank P = 0.84) and overall survival (log rank P = 0.74) between patients with and without PIK3CA mutations. Conclusions Mutations in PIK3CA did not correlate with prognosis in patients with gastric cancer, providing additional evidence for the lack of relationship between the two. PIK3CA mutation (dpeaa)DE-He213 Gastric cancer (dpeaa)DE-He213 Pyrosequencing (dpeaa)DE-He213 Prognosis (dpeaa)DE-He213 Baba, Yoshifumi aut Shigaki, Hironobu aut Ishimoto, Takatsugu aut Miyake, Keisuke aut Kosumi, Keisuke aut Tokunaga, Ryuma aut Izumi, Daisuke aut Ohuchi, Mayuko aut Nakamura, Kenichi aut Kiyozumi, Yuki aut Kurashige, Junji aut Iwatsuki, Masaaki aut Miyamoto, Yuji aut Sakamoto, Yasuo aut Yoshida, Naoya aut Watanabe, Masayuki aut Baba, Hideo aut Enthalten in BMC cancer London : BioMed Central, 2001 16(2016), 1 vom: 07. Juli (DE-627)326643710 (DE-600)2041352-X 1471-2407 nnns volume:16 year:2016 number:1 day:07 month:07 https://dx.doi.org/10.1186/s12885-016-2422-y kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 16 2016 1 07 07
allfields_unstemmed	10.1186/s12885-016-2422-y doi (DE-627)SPR027676935 (SPR)s12885-016-2422-y-e DE-627 ger DE-627 rakwb eng Harada, Kazuto verfasserin aut Prognostic and clinical impact of PIK3CA mutation in gastric cancer: pyrosequencing technology and literature review 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2016 Background Phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha (PIK3CA) mutations that activate the PI3K/AKT signaling pathway have been observed in several types of carcinoma and have been associated with patient prognosis. However, the significance of PIK3CA mutations in gastric cancer remains unclear. This retrospective study investigated the relationship between PIK3CA mutations and clinical outcomes in patients with gastric cancer. Additionally, we reviewed the rate of PIK3CA mutations in gastric cancer and the association between PIK3CA mutations and prognosis in human cancers. Methods The study included 208 patients with gastric cancer who underwent surgical resection at Kumamoto University Hospital, Japan, between January 2001 and August 2010. Mutations in PIK3CA exons 9 and 20 were quantified by pyrosequencing assays. Results PIK3CA mutations were detected in 25 (12 %) of the 208 patients. Ten patients had c.1634A > G (p.E545G), 10 had c.1624G > A (p.E542K), 13 had c.1633G > A (p.E545K), nine had c.3139C > T (p.H1047R), and 1 had c.3140A > G (p.H1047Y) mutations. PIK3CA mutations were not significantly associated with any clinical, epidemiologic, or pathologic characteristic. Kaplan–Meier analysis showed no significant differences in disease-free survival (log rank P = 0.84) and overall survival (log rank P = 0.74) between patients with and without PIK3CA mutations. Conclusions Mutations in PIK3CA did not correlate with prognosis in patients with gastric cancer, providing additional evidence for the lack of relationship between the two. PIK3CA mutation (dpeaa)DE-He213 Gastric cancer (dpeaa)DE-He213 Pyrosequencing (dpeaa)DE-He213 Prognosis (dpeaa)DE-He213 Baba, Yoshifumi aut Shigaki, Hironobu aut Ishimoto, Takatsugu aut Miyake, Keisuke aut Kosumi, Keisuke aut Tokunaga, Ryuma aut Izumi, Daisuke aut Ohuchi, Mayuko aut Nakamura, Kenichi aut Kiyozumi, Yuki aut Kurashige, Junji aut Iwatsuki, Masaaki aut Miyamoto, Yuji aut Sakamoto, Yasuo aut Yoshida, Naoya aut Watanabe, Masayuki aut Baba, Hideo aut Enthalten in BMC cancer London : BioMed Central, 2001 16(2016), 1 vom: 07. Juli (DE-627)326643710 (DE-600)2041352-X 1471-2407 nnns volume:16 year:2016 number:1 day:07 month:07 https://dx.doi.org/10.1186/s12885-016-2422-y kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 16 2016 1 07 07
allfieldsGer	10.1186/s12885-016-2422-y doi (DE-627)SPR027676935 (SPR)s12885-016-2422-y-e DE-627 ger DE-627 rakwb eng Harada, Kazuto verfasserin aut Prognostic and clinical impact of PIK3CA mutation in gastric cancer: pyrosequencing technology and literature review 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2016 Background Phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha (PIK3CA) mutations that activate the PI3K/AKT signaling pathway have been observed in several types of carcinoma and have been associated with patient prognosis. However, the significance of PIK3CA mutations in gastric cancer remains unclear. This retrospective study investigated the relationship between PIK3CA mutations and clinical outcomes in patients with gastric cancer. Additionally, we reviewed the rate of PIK3CA mutations in gastric cancer and the association between PIK3CA mutations and prognosis in human cancers. Methods The study included 208 patients with gastric cancer who underwent surgical resection at Kumamoto University Hospital, Japan, between January 2001 and August 2010. Mutations in PIK3CA exons 9 and 20 were quantified by pyrosequencing assays. Results PIK3CA mutations were detected in 25 (12 %) of the 208 patients. Ten patients had c.1634A > G (p.E545G), 10 had c.1624G > A (p.E542K), 13 had c.1633G > A (p.E545K), nine had c.3139C > T (p.H1047R), and 1 had c.3140A > G (p.H1047Y) mutations. PIK3CA mutations were not significantly associated with any clinical, epidemiologic, or pathologic characteristic. Kaplan–Meier analysis showed no significant differences in disease-free survival (log rank P = 0.84) and overall survival (log rank P = 0.74) between patients with and without PIK3CA mutations. Conclusions Mutations in PIK3CA did not correlate with prognosis in patients with gastric cancer, providing additional evidence for the lack of relationship between the two. PIK3CA mutation (dpeaa)DE-He213 Gastric cancer (dpeaa)DE-He213 Pyrosequencing (dpeaa)DE-He213 Prognosis (dpeaa)DE-He213 Baba, Yoshifumi aut Shigaki, Hironobu aut Ishimoto, Takatsugu aut Miyake, Keisuke aut Kosumi, Keisuke aut Tokunaga, Ryuma aut Izumi, Daisuke aut Ohuchi, Mayuko aut Nakamura, Kenichi aut Kiyozumi, Yuki aut Kurashige, Junji aut Iwatsuki, Masaaki aut Miyamoto, Yuji aut Sakamoto, Yasuo aut Yoshida, Naoya aut Watanabe, Masayuki aut Baba, Hideo aut Enthalten in BMC cancer London : BioMed Central, 2001 16(2016), 1 vom: 07. Juli (DE-627)326643710 (DE-600)2041352-X 1471-2407 nnns volume:16 year:2016 number:1 day:07 month:07 https://dx.doi.org/10.1186/s12885-016-2422-y kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 16 2016 1 07 07
allfieldsSound	10.1186/s12885-016-2422-y doi (DE-627)SPR027676935 (SPR)s12885-016-2422-y-e DE-627 ger DE-627 rakwb eng Harada, Kazuto verfasserin aut Prognostic and clinical impact of PIK3CA mutation in gastric cancer: pyrosequencing technology and literature review 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2016 Background Phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha (PIK3CA) mutations that activate the PI3K/AKT signaling pathway have been observed in several types of carcinoma and have been associated with patient prognosis. However, the significance of PIK3CA mutations in gastric cancer remains unclear. This retrospective study investigated the relationship between PIK3CA mutations and clinical outcomes in patients with gastric cancer. Additionally, we reviewed the rate of PIK3CA mutations in gastric cancer and the association between PIK3CA mutations and prognosis in human cancers. Methods The study included 208 patients with gastric cancer who underwent surgical resection at Kumamoto University Hospital, Japan, between January 2001 and August 2010. Mutations in PIK3CA exons 9 and 20 were quantified by pyrosequencing assays. Results PIK3CA mutations were detected in 25 (12 %) of the 208 patients. Ten patients had c.1634A > G (p.E545G), 10 had c.1624G > A (p.E542K), 13 had c.1633G > A (p.E545K), nine had c.3139C > T (p.H1047R), and 1 had c.3140A > G (p.H1047Y) mutations. PIK3CA mutations were not significantly associated with any clinical, epidemiologic, or pathologic characteristic. Kaplan–Meier analysis showed no significant differences in disease-free survival (log rank P = 0.84) and overall survival (log rank P = 0.74) between patients with and without PIK3CA mutations. Conclusions Mutations in PIK3CA did not correlate with prognosis in patients with gastric cancer, providing additional evidence for the lack of relationship between the two. PIK3CA mutation (dpeaa)DE-He213 Gastric cancer (dpeaa)DE-He213 Pyrosequencing (dpeaa)DE-He213 Prognosis (dpeaa)DE-He213 Baba, Yoshifumi aut Shigaki, Hironobu aut Ishimoto, Takatsugu aut Miyake, Keisuke aut Kosumi, Keisuke aut Tokunaga, Ryuma aut Izumi, Daisuke aut Ohuchi, Mayuko aut Nakamura, Kenichi aut Kiyozumi, Yuki aut Kurashige, Junji aut Iwatsuki, Masaaki aut Miyamoto, Yuji aut Sakamoto, Yasuo aut Yoshida, Naoya aut Watanabe, Masayuki aut Baba, Hideo aut Enthalten in BMC cancer London : BioMed Central, 2001 16(2016), 1 vom: 07. Juli (DE-627)326643710 (DE-600)2041352-X 1471-2407 nnns volume:16 year:2016 number:1 day:07 month:07 https://dx.doi.org/10.1186/s12885-016-2422-y kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 16 2016 1 07 07
language	English
source	Enthalten in BMC cancer 16(2016), 1 vom: 07. Juli volume:16 year:2016 number:1 day:07 month:07
sourceStr	Enthalten in BMC cancer 16(2016), 1 vom: 07. Juli volume:16 year:2016 number:1 day:07 month:07
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	PIK3CA mutation Gastric cancer Pyrosequencing Prognosis
isfreeaccess_bool	true
container_title	BMC cancer
authorswithroles_txt_mv	Harada, Kazuto @@aut@@ Baba, Yoshifumi @@aut@@ Shigaki, Hironobu @@aut@@ Ishimoto, Takatsugu @@aut@@ Miyake, Keisuke @@aut@@ Kosumi, Keisuke @@aut@@ Tokunaga, Ryuma @@aut@@ Izumi, Daisuke @@aut@@ Ohuchi, Mayuko @@aut@@ Nakamura, Kenichi @@aut@@ Kiyozumi, Yuki @@aut@@ Kurashige, Junji @@aut@@ Iwatsuki, Masaaki @@aut@@ Miyamoto, Yuji @@aut@@ Sakamoto, Yasuo @@aut@@ Yoshida, Naoya @@aut@@ Watanabe, Masayuki @@aut@@ Baba, Hideo @@aut@@
publishDateDaySort_date	2016-07-07T00:00:00Z
hierarchy_top_id	326643710
id	SPR027676935
language_de	englisch
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However, the significance of PIK3CA mutations in gastric cancer remains unclear. This retrospective study investigated the relationship between PIK3CA mutations and clinical outcomes in patients with gastric cancer. Additionally, we reviewed the rate of PIK3CA mutations in gastric cancer and the association between PIK3CA mutations and prognosis in human cancers. Methods The study included 208 patients with gastric cancer who underwent surgical resection at Kumamoto University Hospital, Japan, between January 2001 and August 2010. Mutations in PIK3CA exons 9 and 20 were quantified by pyrosequencing assays. Results PIK3CA mutations were detected in 25 (12 %) of the 208 patients. Ten patients had c.1634A > G (p.E545G), 10 had c.1624G > A (p.E542K), 13 had c.1633G > A (p.E545K), nine had c.3139C > T (p.H1047R), and 1 had c.3140A > G (p.H1047Y) mutations. PIK3CA mutations were not significantly associated with any clinical, epidemiologic, or pathologic characteristic. 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author	Harada, Kazuto
spellingShingle	Harada, Kazuto misc PIK3CA mutation misc Gastric cancer misc Pyrosequencing misc Prognosis Prognostic and clinical impact of PIK3CA mutation in gastric cancer: pyrosequencing technology and literature review
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topic_title	Prognostic and clinical impact of PIK3CA mutation in gastric cancer: pyrosequencing technology and literature review PIK3CA mutation (dpeaa)DE-He213 Gastric cancer (dpeaa)DE-He213 Pyrosequencing (dpeaa)DE-He213 Prognosis (dpeaa)DE-He213
topic	misc PIK3CA mutation misc Gastric cancer misc Pyrosequencing misc Prognosis
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title	Prognostic and clinical impact of PIK3CA mutation in gastric cancer: pyrosequencing technology and literature review
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title_full	Prognostic and clinical impact of PIK3CA mutation in gastric cancer: pyrosequencing technology and literature review
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author_browse	Harada, Kazuto Baba, Yoshifumi Shigaki, Hironobu Ishimoto, Takatsugu Miyake, Keisuke Kosumi, Keisuke Tokunaga, Ryuma Izumi, Daisuke Ohuchi, Mayuko Nakamura, Kenichi Kiyozumi, Yuki Kurashige, Junji Iwatsuki, Masaaki Miyamoto, Yuji Sakamoto, Yasuo Yoshida, Naoya Watanabe, Masayuki Baba, Hideo
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title_sort	prognostic and clinical impact of pik3ca mutation in gastric cancer: pyrosequencing technology and literature review
title_auth	Prognostic and clinical impact of PIK3CA mutation in gastric cancer: pyrosequencing technology and literature review
abstract	Background Phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha (PIK3CA) mutations that activate the PI3K/AKT signaling pathway have been observed in several types of carcinoma and have been associated with patient prognosis. However, the significance of PIK3CA mutations in gastric cancer remains unclear. This retrospective study investigated the relationship between PIK3CA mutations and clinical outcomes in patients with gastric cancer. Additionally, we reviewed the rate of PIK3CA mutations in gastric cancer and the association between PIK3CA mutations and prognosis in human cancers. Methods The study included 208 patients with gastric cancer who underwent surgical resection at Kumamoto University Hospital, Japan, between January 2001 and August 2010. Mutations in PIK3CA exons 9 and 20 were quantified by pyrosequencing assays. Results PIK3CA mutations were detected in 25 (12 %) of the 208 patients. Ten patients had c.1634A > G (p.E545G), 10 had c.1624G > A (p.E542K), 13 had c.1633G > A (p.E545K), nine had c.3139C > T (p.H1047R), and 1 had c.3140A > G (p.H1047Y) mutations. PIK3CA mutations were not significantly associated with any clinical, epidemiologic, or pathologic characteristic. Kaplan–Meier analysis showed no significant differences in disease-free survival (log rank P = 0.84) and overall survival (log rank P = 0.74) between patients with and without PIK3CA mutations. Conclusions Mutations in PIK3CA did not correlate with prognosis in patients with gastric cancer, providing additional evidence for the lack of relationship between the two. © The Author(s). 2016
abstractGer	Background Phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha (PIK3CA) mutations that activate the PI3K/AKT signaling pathway have been observed in several types of carcinoma and have been associated with patient prognosis. However, the significance of PIK3CA mutations in gastric cancer remains unclear. This retrospective study investigated the relationship between PIK3CA mutations and clinical outcomes in patients with gastric cancer. Additionally, we reviewed the rate of PIK3CA mutations in gastric cancer and the association between PIK3CA mutations and prognosis in human cancers. Methods The study included 208 patients with gastric cancer who underwent surgical resection at Kumamoto University Hospital, Japan, between January 2001 and August 2010. Mutations in PIK3CA exons 9 and 20 were quantified by pyrosequencing assays. Results PIK3CA mutations were detected in 25 (12 %) of the 208 patients. Ten patients had c.1634A > G (p.E545G), 10 had c.1624G > A (p.E542K), 13 had c.1633G > A (p.E545K), nine had c.3139C > T (p.H1047R), and 1 had c.3140A > G (p.H1047Y) mutations. PIK3CA mutations were not significantly associated with any clinical, epidemiologic, or pathologic characteristic. Kaplan–Meier analysis showed no significant differences in disease-free survival (log rank P = 0.84) and overall survival (log rank P = 0.74) between patients with and without PIK3CA mutations. Conclusions Mutations in PIK3CA did not correlate with prognosis in patients with gastric cancer, providing additional evidence for the lack of relationship between the two. © The Author(s). 2016
abstract_unstemmed	Background Phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha (PIK3CA) mutations that activate the PI3K/AKT signaling pathway have been observed in several types of carcinoma and have been associated with patient prognosis. However, the significance of PIK3CA mutations in gastric cancer remains unclear. This retrospective study investigated the relationship between PIK3CA mutations and clinical outcomes in patients with gastric cancer. Additionally, we reviewed the rate of PIK3CA mutations in gastric cancer and the association between PIK3CA mutations and prognosis in human cancers. Methods The study included 208 patients with gastric cancer who underwent surgical resection at Kumamoto University Hospital, Japan, between January 2001 and August 2010. Mutations in PIK3CA exons 9 and 20 were quantified by pyrosequencing assays. Results PIK3CA mutations were detected in 25 (12 %) of the 208 patients. Ten patients had c.1634A > G (p.E545G), 10 had c.1624G > A (p.E542K), 13 had c.1633G > A (p.E545K), nine had c.3139C > T (p.H1047R), and 1 had c.3140A > G (p.H1047Y) mutations. PIK3CA mutations were not significantly associated with any clinical, epidemiologic, or pathologic characteristic. Kaplan–Meier analysis showed no significant differences in disease-free survival (log rank P = 0.84) and overall survival (log rank P = 0.74) between patients with and without PIK3CA mutations. Conclusions Mutations in PIK3CA did not correlate with prognosis in patients with gastric cancer, providing additional evidence for the lack of relationship between the two. © The Author(s). 2016
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title_short	Prognostic and clinical impact of PIK3CA mutation in gastric cancer: pyrosequencing technology and literature review
url	https://dx.doi.org/10.1186/s12885-016-2422-y
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author2	Baba, Yoshifumi Shigaki, Hironobu Ishimoto, Takatsugu Miyake, Keisuke Kosumi, Keisuke Tokunaga, Ryuma Izumi, Daisuke Ohuchi, Mayuko Nakamura, Kenichi Kiyozumi, Yuki Kurashige, Junji Iwatsuki, Masaaki Miyamoto, Yuji Sakamoto, Yasuo Yoshida, Naoya Watanabe, Masayuki Baba, Hideo
author2Str	Baba, Yoshifumi Shigaki, Hironobu Ishimoto, Takatsugu Miyake, Keisuke Kosumi, Keisuke Tokunaga, Ryuma Izumi, Daisuke Ohuchi, Mayuko Nakamura, Kenichi Kiyozumi, Yuki Kurashige, Junji Iwatsuki, Masaaki Miyamoto, Yuji Sakamoto, Yasuo Yoshida, Naoya Watanabe, Masayuki Baba, Hideo
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