In vitro and in vivo antidermatophytic activity of the dichloromethane-methanol (1:1 v/v) extract from the stem bark of Polyscias fulva Hiern (Araliaceae)

Background During the last decades, the number of people suffering from dermatophytoses has seriously increased, mainly due to the development of resistant strains of microorganisms to a range of formally efficient antibiotics. Polyscias fulva, a medium size tree which grows in the West Region of Ca...
Ausführliche Beschreibung

Background During the last decades, the number of people suffering from dermatophytoses has seriously increased, mainly due to the development of resistant strains of microorganisms to a range of formally efficient antibiotics. Polyscias fulva, a medium size tree which grows in the West Region of Cameroon is traditionally used for local application against dermatoses and orally against venereal infections. The dichloromethane-methanol (1:1 v/v) extract from the stem bark of Polyscias fulva was evaluated for its in vitro and in vivo antifungal activities. Methods The plant extract was prepared by maceration of its stem bark powder in $ CH_{2} %$ Cl_{2} $-MeOH (1:1 v/v). The extract obtained was successively partitioned in hexane, ethyl acetate and n-butanol. Phytochemical screening was performed using standard methods. In vitro antidermatophytic activity was assayed by the well diffusion and broth microdilution methods. The degree of dermal irritation of the crude extract was determined in guinea pigs using the occluded dermal irritation test method. The in vivo antidermatophytic activity of the extract-oil formulation (1.25, 2.5 and 5% w/w concentrations) was evaluated using Trichophyton mentagrophytes- induced dermatophytosis in a guinea pigs model. Results Phytochemical screening indicated that, the crude extract, ethyl acetate, n-butanol and residue fractions contain in general saponins, tannins, alkaloids, anthraquinones and phenols while the hexane fraction contains only alkaloids. The ethyl-acetate, n-butanol and residue fractions displayed higher antifungal activities (MIC = 0.125-0.5 mg.$ mL^{-1} $) against eight dermatophytes as compared to the crude extract (MIC = 0.5-1 mg.$ mL^{-1} $). This latter appeared to have slight perceptible erythema effects on guinea pigs as the primary irritation index (PII) was calculated to be 0.54. In vivo, the antidermatophytic activities of the extract-oil formulations were dose-dependent. Griseofulvin-oil 5% at 0.01 g/kg and formulated extract-oil (5%) at 0.1 g/kg eradicated the microbial infection after thirteen and fourteen days of daily treatment respectively. Conclusions The results of preclinical in vitro and in vivo evaluations indicate that the extract-oil formulation at 5% may constitute an alternative means to alleviate fungal infections caused by dermatophytes. Ausführliche Beschreibung

Autor*in:	Njateng, Guy Sedar Singor [verfasserIn] Gatsing, Donatien Mouokeu, Raymond Simplice Lunga, Paul Keilah Kuiate, Jules-Roger

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2013

Schlagwörter:	Antidermatophytic activity Polyscias fulva Extract-oil formulation Irritation test Primary irritation index Guinea pigs

Anmerkung:	© Njateng et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (

Übergeordnetes Werk:	Enthalten in: BMC complementary and alternative medicine - London : BioMed Central, 2001, 13(2013), 1 vom: 06. Mai
Übergeordnetes Werk:	volume:13 ; year:2013 ; number:1 ; day:06 ; month:05

Links:	Volltext

DOI / URN:	10.1186/1472-6882-13-95

Katalog-ID:	SPR028121716