Optimization of an E. coli L-rhamnose-inducible expression vector: test of various genetic module combinations

Background A capable expression vector is mainly characterized by its production efficiency, stability and induction response. These features can be influenced by a variation of modifications and versatile genetic modules. Results We examined miscellaneous variations of a rhaPBADexpression vector. T...
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Autor*in:	Wegerer, Angelika [verfasserIn] Sun, Tianqi Altenbuchner, Josef

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2008

Schlagwörter:	Genetic Module eGFP Expression Plasmid Stability Stem Loop Plasmid Loss

Anmerkung:	© Wegerer et al; licensee BioMed Central Ltd. 2008. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (

Übergeordnetes Werk:	Enthalten in: BMC biotechnology - London : BioMed Central, 2001, 8(2008), 1 vom: 14. Jan.
Übergeordnetes Werk:	volume:8 ; year:2008 ; number:1 ; day:14 ; month:01

Links:	Volltext

DOI / URN:	10.1186/1472-6750-8-2

Katalog-ID:	SPR028348729

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520			\|a Background A capable expression vector is mainly characterized by its production efficiency, stability and induction response. These features can be influenced by a variation of modifications and versatile genetic modules. Results We examined miscellaneous variations of a rhaPBADexpression vector. The introduction of a stem loop into the translation initiation region of the rhaPBADpromoter resulted in the most significant improvement of eGFP expression. Starting from this plasmid, we constructed a set of expression vectors bearing different genetic modules like rop, ccdAB, cer and combinations thereof, and tested the efficiency of expression and plasmid stability. The plasmid pWA21, containing the stem loop, one cer site and rop, attained high expression levels accompanied by a good stability, and on that score seems to be a well-balanced choice. Conclusion We report the generation of variations of the rhaPBADexpression vector and characterization hereof. The genetic modules showed a complex interplay, therefore two positive effects combined sometimes resulted in a disadvantage.
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allfields	10.1186/1472-6750-8-2 doi (DE-627)SPR028348729 (SPR)1472-6750-8-2-e DE-627 ger DE-627 rakwb eng Wegerer, Angelika verfasserin aut Optimization of an E. coli L-rhamnose-inducible expression vector: test of various genetic module combinations 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Wegerer et al; licensee BioMed Central Ltd. 2008. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( Background A capable expression vector is mainly characterized by its production efficiency, stability and induction response. These features can be influenced by a variation of modifications and versatile genetic modules. Results We examined miscellaneous variations of a rhaPBADexpression vector. The introduction of a stem loop into the translation initiation region of the rhaPBADpromoter resulted in the most significant improvement of eGFP expression. Starting from this plasmid, we constructed a set of expression vectors bearing different genetic modules like rop, ccdAB, cer and combinations thereof, and tested the efficiency of expression and plasmid stability. The plasmid pWA21, containing the stem loop, one cer site and rop, attained high expression levels accompanied by a good stability, and on that score seems to be a well-balanced choice. Conclusion We report the generation of variations of the rhaPBADexpression vector and characterization hereof. The genetic modules showed a complex interplay, therefore two positive effects combined sometimes resulted in a disadvantage. Genetic Module (dpeaa)DE-He213 eGFP Expression (dpeaa)DE-He213 Plasmid Stability (dpeaa)DE-He213 Stem Loop (dpeaa)DE-He213 Plasmid Loss (dpeaa)DE-He213 Sun, Tianqi aut Altenbuchner, Josef aut Enthalten in BMC biotechnology London : BioMed Central, 2001 8(2008), 1 vom: 14. Jan. (DE-627)332164837 (DE-600)2052746-9 1472-6750 nnns volume:8 year:2008 number:1 day:14 month:01 https://dx.doi.org/10.1186/1472-6750-8-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2119 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2008 1 14 01
spelling	10.1186/1472-6750-8-2 doi (DE-627)SPR028348729 (SPR)1472-6750-8-2-e DE-627 ger DE-627 rakwb eng Wegerer, Angelika verfasserin aut Optimization of an E. coli L-rhamnose-inducible expression vector: test of various genetic module combinations 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Wegerer et al; licensee BioMed Central Ltd. 2008. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( Background A capable expression vector is mainly characterized by its production efficiency, stability and induction response. These features can be influenced by a variation of modifications and versatile genetic modules. Results We examined miscellaneous variations of a rhaPBADexpression vector. The introduction of a stem loop into the translation initiation region of the rhaPBADpromoter resulted in the most significant improvement of eGFP expression. Starting from this plasmid, we constructed a set of expression vectors bearing different genetic modules like rop, ccdAB, cer and combinations thereof, and tested the efficiency of expression and plasmid stability. The plasmid pWA21, containing the stem loop, one cer site and rop, attained high expression levels accompanied by a good stability, and on that score seems to be a well-balanced choice. Conclusion We report the generation of variations of the rhaPBADexpression vector and characterization hereof. The genetic modules showed a complex interplay, therefore two positive effects combined sometimes resulted in a disadvantage. Genetic Module (dpeaa)DE-He213 eGFP Expression (dpeaa)DE-He213 Plasmid Stability (dpeaa)DE-He213 Stem Loop (dpeaa)DE-He213 Plasmid Loss (dpeaa)DE-He213 Sun, Tianqi aut Altenbuchner, Josef aut Enthalten in BMC biotechnology London : BioMed Central, 2001 8(2008), 1 vom: 14. Jan. (DE-627)332164837 (DE-600)2052746-9 1472-6750 nnns volume:8 year:2008 number:1 day:14 month:01 https://dx.doi.org/10.1186/1472-6750-8-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2119 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2008 1 14 01
allfields_unstemmed	10.1186/1472-6750-8-2 doi (DE-627)SPR028348729 (SPR)1472-6750-8-2-e DE-627 ger DE-627 rakwb eng Wegerer, Angelika verfasserin aut Optimization of an E. coli L-rhamnose-inducible expression vector: test of various genetic module combinations 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Wegerer et al; licensee BioMed Central Ltd. 2008. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( Background A capable expression vector is mainly characterized by its production efficiency, stability and induction response. These features can be influenced by a variation of modifications and versatile genetic modules. Results We examined miscellaneous variations of a rhaPBADexpression vector. The introduction of a stem loop into the translation initiation region of the rhaPBADpromoter resulted in the most significant improvement of eGFP expression. Starting from this plasmid, we constructed a set of expression vectors bearing different genetic modules like rop, ccdAB, cer and combinations thereof, and tested the efficiency of expression and plasmid stability. The plasmid pWA21, containing the stem loop, one cer site and rop, attained high expression levels accompanied by a good stability, and on that score seems to be a well-balanced choice. Conclusion We report the generation of variations of the rhaPBADexpression vector and characterization hereof. The genetic modules showed a complex interplay, therefore two positive effects combined sometimes resulted in a disadvantage. Genetic Module (dpeaa)DE-He213 eGFP Expression (dpeaa)DE-He213 Plasmid Stability (dpeaa)DE-He213 Stem Loop (dpeaa)DE-He213 Plasmid Loss (dpeaa)DE-He213 Sun, Tianqi aut Altenbuchner, Josef aut Enthalten in BMC biotechnology London : BioMed Central, 2001 8(2008), 1 vom: 14. Jan. (DE-627)332164837 (DE-600)2052746-9 1472-6750 nnns volume:8 year:2008 number:1 day:14 month:01 https://dx.doi.org/10.1186/1472-6750-8-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2119 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2008 1 14 01
allfieldsGer	10.1186/1472-6750-8-2 doi (DE-627)SPR028348729 (SPR)1472-6750-8-2-e DE-627 ger DE-627 rakwb eng Wegerer, Angelika verfasserin aut Optimization of an E. coli L-rhamnose-inducible expression vector: test of various genetic module combinations 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Wegerer et al; licensee BioMed Central Ltd. 2008. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( Background A capable expression vector is mainly characterized by its production efficiency, stability and induction response. These features can be influenced by a variation of modifications and versatile genetic modules. Results We examined miscellaneous variations of a rhaPBADexpression vector. The introduction of a stem loop into the translation initiation region of the rhaPBADpromoter resulted in the most significant improvement of eGFP expression. Starting from this plasmid, we constructed a set of expression vectors bearing different genetic modules like rop, ccdAB, cer and combinations thereof, and tested the efficiency of expression and plasmid stability. The plasmid pWA21, containing the stem loop, one cer site and rop, attained high expression levels accompanied by a good stability, and on that score seems to be a well-balanced choice. Conclusion We report the generation of variations of the rhaPBADexpression vector and characterization hereof. The genetic modules showed a complex interplay, therefore two positive effects combined sometimes resulted in a disadvantage. Genetic Module (dpeaa)DE-He213 eGFP Expression (dpeaa)DE-He213 Plasmid Stability (dpeaa)DE-He213 Stem Loop (dpeaa)DE-He213 Plasmid Loss (dpeaa)DE-He213 Sun, Tianqi aut Altenbuchner, Josef aut Enthalten in BMC biotechnology London : BioMed Central, 2001 8(2008), 1 vom: 14. Jan. (DE-627)332164837 (DE-600)2052746-9 1472-6750 nnns volume:8 year:2008 number:1 day:14 month:01 https://dx.doi.org/10.1186/1472-6750-8-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2119 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2008 1 14 01
allfieldsSound	10.1186/1472-6750-8-2 doi (DE-627)SPR028348729 (SPR)1472-6750-8-2-e DE-627 ger DE-627 rakwb eng Wegerer, Angelika verfasserin aut Optimization of an E. coli L-rhamnose-inducible expression vector: test of various genetic module combinations 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Wegerer et al; licensee BioMed Central Ltd. 2008. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( Background A capable expression vector is mainly characterized by its production efficiency, stability and induction response. These features can be influenced by a variation of modifications and versatile genetic modules. Results We examined miscellaneous variations of a rhaPBADexpression vector. The introduction of a stem loop into the translation initiation region of the rhaPBADpromoter resulted in the most significant improvement of eGFP expression. Starting from this plasmid, we constructed a set of expression vectors bearing different genetic modules like rop, ccdAB, cer and combinations thereof, and tested the efficiency of expression and plasmid stability. The plasmid pWA21, containing the stem loop, one cer site and rop, attained high expression levels accompanied by a good stability, and on that score seems to be a well-balanced choice. Conclusion We report the generation of variations of the rhaPBADexpression vector and characterization hereof. The genetic modules showed a complex interplay, therefore two positive effects combined sometimes resulted in a disadvantage. Genetic Module (dpeaa)DE-He213 eGFP Expression (dpeaa)DE-He213 Plasmid Stability (dpeaa)DE-He213 Stem Loop (dpeaa)DE-He213 Plasmid Loss (dpeaa)DE-He213 Sun, Tianqi aut Altenbuchner, Josef aut Enthalten in BMC biotechnology London : BioMed Central, 2001 8(2008), 1 vom: 14. Jan. (DE-627)332164837 (DE-600)2052746-9 1472-6750 nnns volume:8 year:2008 number:1 day:14 month:01 https://dx.doi.org/10.1186/1472-6750-8-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2119 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2008 1 14 01
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source	Enthalten in BMC biotechnology 8(2008), 1 vom: 14. Jan. volume:8 year:2008 number:1 day:14 month:01
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author	Wegerer, Angelika
spellingShingle	Wegerer, Angelika misc Genetic Module misc eGFP Expression misc Plasmid Stability misc Stem Loop misc Plasmid Loss Optimization of an E. coli L-rhamnose-inducible expression vector: test of various genetic module combinations
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topic_title	Optimization of an E. coli L-rhamnose-inducible expression vector: test of various genetic module combinations Genetic Module (dpeaa)DE-He213 eGFP Expression (dpeaa)DE-He213 Plasmid Stability (dpeaa)DE-He213 Stem Loop (dpeaa)DE-He213 Plasmid Loss (dpeaa)DE-He213
topic	misc Genetic Module misc eGFP Expression misc Plasmid Stability misc Stem Loop misc Plasmid Loss
topic_unstemmed	misc Genetic Module misc eGFP Expression misc Plasmid Stability misc Stem Loop misc Plasmid Loss
topic_browse	misc Genetic Module misc eGFP Expression misc Plasmid Stability misc Stem Loop misc Plasmid Loss
format_facet	Elektronische Aufsätze Aufsätze Elektronische Ressource
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title	Optimization of an E. coli L-rhamnose-inducible expression vector: test of various genetic module combinations
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title_full	Optimization of an E. coli L-rhamnose-inducible expression vector: test of various genetic module combinations
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author_browse	Wegerer, Angelika Sun, Tianqi Altenbuchner, Josef
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doi_str_mv	10.1186/1472-6750-8-2
title_sort	optimization of an e. coli l-rhamnose-inducible expression vector: test of various genetic module combinations
title_auth	Optimization of an E. coli L-rhamnose-inducible expression vector: test of various genetic module combinations
abstract	Background A capable expression vector is mainly characterized by its production efficiency, stability and induction response. These features can be influenced by a variation of modifications and versatile genetic modules. Results We examined miscellaneous variations of a rhaPBADexpression vector. The introduction of a stem loop into the translation initiation region of the rhaPBADpromoter resulted in the most significant improvement of eGFP expression. Starting from this plasmid, we constructed a set of expression vectors bearing different genetic modules like rop, ccdAB, cer and combinations thereof, and tested the efficiency of expression and plasmid stability. The plasmid pWA21, containing the stem loop, one cer site and rop, attained high expression levels accompanied by a good stability, and on that score seems to be a well-balanced choice. Conclusion We report the generation of variations of the rhaPBADexpression vector and characterization hereof. The genetic modules showed a complex interplay, therefore two positive effects combined sometimes resulted in a disadvantage. © Wegerer et al; licensee BioMed Central Ltd. 2008. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (
abstractGer	Background A capable expression vector is mainly characterized by its production efficiency, stability and induction response. These features can be influenced by a variation of modifications and versatile genetic modules. Results We examined miscellaneous variations of a rhaPBADexpression vector. The introduction of a stem loop into the translation initiation region of the rhaPBADpromoter resulted in the most significant improvement of eGFP expression. Starting from this plasmid, we constructed a set of expression vectors bearing different genetic modules like rop, ccdAB, cer and combinations thereof, and tested the efficiency of expression and plasmid stability. The plasmid pWA21, containing the stem loop, one cer site and rop, attained high expression levels accompanied by a good stability, and on that score seems to be a well-balanced choice. Conclusion We report the generation of variations of the rhaPBADexpression vector and characterization hereof. The genetic modules showed a complex interplay, therefore two positive effects combined sometimes resulted in a disadvantage. © Wegerer et al; licensee BioMed Central Ltd. 2008. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (
abstract_unstemmed	Background A capable expression vector is mainly characterized by its production efficiency, stability and induction response. These features can be influenced by a variation of modifications and versatile genetic modules. Results We examined miscellaneous variations of a rhaPBADexpression vector. The introduction of a stem loop into the translation initiation region of the rhaPBADpromoter resulted in the most significant improvement of eGFP expression. Starting from this plasmid, we constructed a set of expression vectors bearing different genetic modules like rop, ccdAB, cer and combinations thereof, and tested the efficiency of expression and plasmid stability. The plasmid pWA21, containing the stem loop, one cer site and rop, attained high expression levels accompanied by a good stability, and on that score seems to be a well-balanced choice. Conclusion We report the generation of variations of the rhaPBADexpression vector and characterization hereof. The genetic modules showed a complex interplay, therefore two positive effects combined sometimes resulted in a disadvantage. © Wegerer et al; licensee BioMed Central Ltd. 2008. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (
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title_short	Optimization of an E. coli L-rhamnose-inducible expression vector: test of various genetic module combinations
url	https://dx.doi.org/10.1186/1472-6750-8-2
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This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Background A capable expression vector is mainly characterized by its production efficiency, stability and induction response. These features can be influenced by a variation of modifications and versatile genetic modules. Results We examined miscellaneous variations of a rhaPBADexpression vector. The introduction of a stem loop into the translation initiation region of the rhaPBADpromoter resulted in the most significant improvement of eGFP expression. Starting from this plasmid, we constructed a set of expression vectors bearing different genetic modules like rop, ccdAB, cer and combinations thereof, and tested the efficiency of expression and plasmid stability. The plasmid pWA21, containing the stem loop, one cer site and rop, attained high expression levels accompanied by a good stability, and on that score seems to be a well-balanced choice. Conclusion We report the generation of variations of the rhaPBADexpression vector and characterization hereof. 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Optimization of an E. coli L-rhamnose-inducible expression vector: test of various genetic module combinations

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