Serological testing of cattle experimentally infected with Mycoplasmamycoides subsp. mycoides Small Colony using four different tests reveals a variety of seroconversion patterns

Background To study the specific antibody response to infection with Mycoplasma mycoides subsp. mycoides Small Colony (MmmSC), the agent of Contagious Bovine Pleuropneumonia (CBPP), we examined three panels of sera collected during three experimental infection trials in African cattle. The methods u...
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Autor*in:	Schubert, Evelyn [verfasserIn] Sachse, Konrad Jores, Jörg Heller, Martin

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2011

Schlagwörter:	Complement Fixation Test High Responder Lung Tissue Sample Specific Antibody Response Herd Level

Anmerkung:	© Schubert et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (

Übergeordnetes Werk:	Enthalten in: BMC veterinary research - London : BioMed Central, 2005, 7(2011), 1 vom: 18. Nov.
Übergeordnetes Werk:	volume:7 ; year:2011 ; number:1 ; day:18 ; month:11

Links:	Volltext

DOI / URN:	10.1186/1746-6148-7-72

Katalog-ID:	SPR028371720

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520			\|a Background To study the specific antibody response to infection with Mycoplasma mycoides subsp. mycoides Small Colony (MmmSC), the agent of Contagious Bovine Pleuropneumonia (CBPP), we examined three panels of sera collected during three experimental infection trials in African cattle. The methods used included an in-house complement fixation test (CFT), a commercially available CFT, a competitive antibody ELISA (cELISA) and the immunoblotting test (IBT). In addition, lung tissue samples were examined by culture. Results A total of 89% (51/59) of all experimentally infected animals tested positive on at least one of the serological tests throughout the trial. The specific antibody titres to the MmmSC infection became positive first by CFT (6 to 9 days post infection [dpi]), followed by IBT (9 to 13 dpi) and cELISA (13 to 16 dpi). Individual animals were found to display remarkably distinct seroconversion patterns, which allowed their classification into i) early high responders, ii) late high responders, and iii) low responders. In accordance with other studies, none of the present serological tests was capable of detecting all CBPP infected animals. Conclusion Comparison of the assays' performance in terms of sensitivity and specificity raises serious questions as to their reliability for identification of infected individuals in the field. In view of these limitations, a combination of CFT and cELISA can markedly improve CBPP diagnosis at single-animal level.
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allfields	10.1186/1746-6148-7-72 doi (DE-627)SPR028371720 (SPR)1746-6148-7-72-e DE-627 ger DE-627 rakwb eng Schubert, Evelyn verfasserin aut Serological testing of cattle experimentally infected with Mycoplasmamycoides subsp. mycoides Small Colony using four different tests reveals a variety of seroconversion patterns 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Schubert et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( Background To study the specific antibody response to infection with Mycoplasma mycoides subsp. mycoides Small Colony (MmmSC), the agent of Contagious Bovine Pleuropneumonia (CBPP), we examined three panels of sera collected during three experimental infection trials in African cattle. The methods used included an in-house complement fixation test (CFT), a commercially available CFT, a competitive antibody ELISA (cELISA) and the immunoblotting test (IBT). In addition, lung tissue samples were examined by culture. Results A total of 89% (51/59) of all experimentally infected animals tested positive on at least one of the serological tests throughout the trial. The specific antibody titres to the MmmSC infection became positive first by CFT (6 to 9 days post infection [dpi]), followed by IBT (9 to 13 dpi) and cELISA (13 to 16 dpi). Individual animals were found to display remarkably distinct seroconversion patterns, which allowed their classification into i) early high responders, ii) late high responders, and iii) low responders. In accordance with other studies, none of the present serological tests was capable of detecting all CBPP infected animals. Conclusion Comparison of the assays' performance in terms of sensitivity and specificity raises serious questions as to their reliability for identification of infected individuals in the field. In view of these limitations, a combination of CFT and cELISA can markedly improve CBPP diagnosis at single-animal level. Complement Fixation Test (dpeaa)DE-He213 High Responder (dpeaa)DE-He213 Lung Tissue Sample (dpeaa)DE-He213 Specific Antibody Response (dpeaa)DE-He213 Herd Level (dpeaa)DE-He213 Sachse, Konrad aut Jores, Jörg aut Heller, Martin aut Enthalten in BMC veterinary research London : BioMed Central, 2005 7(2011), 1 vom: 18. Nov. (DE-627)489256538 (DE-600)2191675-5 1746-6148 nnns volume:7 year:2011 number:1 day:18 month:11 https://dx.doi.org/10.1186/1746-6148-7-72 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2011 1 18 11
spelling	10.1186/1746-6148-7-72 doi (DE-627)SPR028371720 (SPR)1746-6148-7-72-e DE-627 ger DE-627 rakwb eng Schubert, Evelyn verfasserin aut Serological testing of cattle experimentally infected with Mycoplasmamycoides subsp. mycoides Small Colony using four different tests reveals a variety of seroconversion patterns 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Schubert et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( Background To study the specific antibody response to infection with Mycoplasma mycoides subsp. mycoides Small Colony (MmmSC), the agent of Contagious Bovine Pleuropneumonia (CBPP), we examined three panels of sera collected during three experimental infection trials in African cattle. The methods used included an in-house complement fixation test (CFT), a commercially available CFT, a competitive antibody ELISA (cELISA) and the immunoblotting test (IBT). In addition, lung tissue samples were examined by culture. Results A total of 89% (51/59) of all experimentally infected animals tested positive on at least one of the serological tests throughout the trial. The specific antibody titres to the MmmSC infection became positive first by CFT (6 to 9 days post infection [dpi]), followed by IBT (9 to 13 dpi) and cELISA (13 to 16 dpi). Individual animals were found to display remarkably distinct seroconversion patterns, which allowed their classification into i) early high responders, ii) late high responders, and iii) low responders. In accordance with other studies, none of the present serological tests was capable of detecting all CBPP infected animals. Conclusion Comparison of the assays' performance in terms of sensitivity and specificity raises serious questions as to their reliability for identification of infected individuals in the field. In view of these limitations, a combination of CFT and cELISA can markedly improve CBPP diagnosis at single-animal level. Complement Fixation Test (dpeaa)DE-He213 High Responder (dpeaa)DE-He213 Lung Tissue Sample (dpeaa)DE-He213 Specific Antibody Response (dpeaa)DE-He213 Herd Level (dpeaa)DE-He213 Sachse, Konrad aut Jores, Jörg aut Heller, Martin aut Enthalten in BMC veterinary research London : BioMed Central, 2005 7(2011), 1 vom: 18. Nov. (DE-627)489256538 (DE-600)2191675-5 1746-6148 nnns volume:7 year:2011 number:1 day:18 month:11 https://dx.doi.org/10.1186/1746-6148-7-72 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2011 1 18 11
allfields_unstemmed	10.1186/1746-6148-7-72 doi (DE-627)SPR028371720 (SPR)1746-6148-7-72-e DE-627 ger DE-627 rakwb eng Schubert, Evelyn verfasserin aut Serological testing of cattle experimentally infected with Mycoplasmamycoides subsp. mycoides Small Colony using four different tests reveals a variety of seroconversion patterns 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Schubert et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( Background To study the specific antibody response to infection with Mycoplasma mycoides subsp. mycoides Small Colony (MmmSC), the agent of Contagious Bovine Pleuropneumonia (CBPP), we examined three panels of sera collected during three experimental infection trials in African cattle. The methods used included an in-house complement fixation test (CFT), a commercially available CFT, a competitive antibody ELISA (cELISA) and the immunoblotting test (IBT). In addition, lung tissue samples were examined by culture. Results A total of 89% (51/59) of all experimentally infected animals tested positive on at least one of the serological tests throughout the trial. The specific antibody titres to the MmmSC infection became positive first by CFT (6 to 9 days post infection [dpi]), followed by IBT (9 to 13 dpi) and cELISA (13 to 16 dpi). Individual animals were found to display remarkably distinct seroconversion patterns, which allowed their classification into i) early high responders, ii) late high responders, and iii) low responders. In accordance with other studies, none of the present serological tests was capable of detecting all CBPP infected animals. Conclusion Comparison of the assays' performance in terms of sensitivity and specificity raises serious questions as to their reliability for identification of infected individuals in the field. In view of these limitations, a combination of CFT and cELISA can markedly improve CBPP diagnosis at single-animal level. Complement Fixation Test (dpeaa)DE-He213 High Responder (dpeaa)DE-He213 Lung Tissue Sample (dpeaa)DE-He213 Specific Antibody Response (dpeaa)DE-He213 Herd Level (dpeaa)DE-He213 Sachse, Konrad aut Jores, Jörg aut Heller, Martin aut Enthalten in BMC veterinary research London : BioMed Central, 2005 7(2011), 1 vom: 18. Nov. (DE-627)489256538 (DE-600)2191675-5 1746-6148 nnns volume:7 year:2011 number:1 day:18 month:11 https://dx.doi.org/10.1186/1746-6148-7-72 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2011 1 18 11
allfieldsGer	10.1186/1746-6148-7-72 doi (DE-627)SPR028371720 (SPR)1746-6148-7-72-e DE-627 ger DE-627 rakwb eng Schubert, Evelyn verfasserin aut Serological testing of cattle experimentally infected with Mycoplasmamycoides subsp. mycoides Small Colony using four different tests reveals a variety of seroconversion patterns 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Schubert et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( Background To study the specific antibody response to infection with Mycoplasma mycoides subsp. mycoides Small Colony (MmmSC), the agent of Contagious Bovine Pleuropneumonia (CBPP), we examined three panels of sera collected during three experimental infection trials in African cattle. The methods used included an in-house complement fixation test (CFT), a commercially available CFT, a competitive antibody ELISA (cELISA) and the immunoblotting test (IBT). In addition, lung tissue samples were examined by culture. Results A total of 89% (51/59) of all experimentally infected animals tested positive on at least one of the serological tests throughout the trial. The specific antibody titres to the MmmSC infection became positive first by CFT (6 to 9 days post infection [dpi]), followed by IBT (9 to 13 dpi) and cELISA (13 to 16 dpi). Individual animals were found to display remarkably distinct seroconversion patterns, which allowed their classification into i) early high responders, ii) late high responders, and iii) low responders. In accordance with other studies, none of the present serological tests was capable of detecting all CBPP infected animals. Conclusion Comparison of the assays' performance in terms of sensitivity and specificity raises serious questions as to their reliability for identification of infected individuals in the field. In view of these limitations, a combination of CFT and cELISA can markedly improve CBPP diagnosis at single-animal level. Complement Fixation Test (dpeaa)DE-He213 High Responder (dpeaa)DE-He213 Lung Tissue Sample (dpeaa)DE-He213 Specific Antibody Response (dpeaa)DE-He213 Herd Level (dpeaa)DE-He213 Sachse, Konrad aut Jores, Jörg aut Heller, Martin aut Enthalten in BMC veterinary research London : BioMed Central, 2005 7(2011), 1 vom: 18. Nov. (DE-627)489256538 (DE-600)2191675-5 1746-6148 nnns volume:7 year:2011 number:1 day:18 month:11 https://dx.doi.org/10.1186/1746-6148-7-72 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2011 1 18 11
allfieldsSound	10.1186/1746-6148-7-72 doi (DE-627)SPR028371720 (SPR)1746-6148-7-72-e DE-627 ger DE-627 rakwb eng Schubert, Evelyn verfasserin aut Serological testing of cattle experimentally infected with Mycoplasmamycoides subsp. mycoides Small Colony using four different tests reveals a variety of seroconversion patterns 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Schubert et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( Background To study the specific antibody response to infection with Mycoplasma mycoides subsp. mycoides Small Colony (MmmSC), the agent of Contagious Bovine Pleuropneumonia (CBPP), we examined three panels of sera collected during three experimental infection trials in African cattle. The methods used included an in-house complement fixation test (CFT), a commercially available CFT, a competitive antibody ELISA (cELISA) and the immunoblotting test (IBT). In addition, lung tissue samples were examined by culture. Results A total of 89% (51/59) of all experimentally infected animals tested positive on at least one of the serological tests throughout the trial. The specific antibody titres to the MmmSC infection became positive first by CFT (6 to 9 days post infection [dpi]), followed by IBT (9 to 13 dpi) and cELISA (13 to 16 dpi). Individual animals were found to display remarkably distinct seroconversion patterns, which allowed their classification into i) early high responders, ii) late high responders, and iii) low responders. In accordance with other studies, none of the present serological tests was capable of detecting all CBPP infected animals. Conclusion Comparison of the assays' performance in terms of sensitivity and specificity raises serious questions as to their reliability for identification of infected individuals in the field. In view of these limitations, a combination of CFT and cELISA can markedly improve CBPP diagnosis at single-animal level. Complement Fixation Test (dpeaa)DE-He213 High Responder (dpeaa)DE-He213 Lung Tissue Sample (dpeaa)DE-He213 Specific Antibody Response (dpeaa)DE-He213 Herd Level (dpeaa)DE-He213 Sachse, Konrad aut Jores, Jörg aut Heller, Martin aut Enthalten in BMC veterinary research London : BioMed Central, 2005 7(2011), 1 vom: 18. Nov. (DE-627)489256538 (DE-600)2191675-5 1746-6148 nnns volume:7 year:2011 number:1 day:18 month:11 https://dx.doi.org/10.1186/1746-6148-7-72 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2011 1 18 11
language	English
source	Enthalten in BMC veterinary research 7(2011), 1 vom: 18. Nov. volume:7 year:2011 number:1 day:18 month:11
sourceStr	Enthalten in BMC veterinary research 7(2011), 1 vom: 18. Nov. volume:7 year:2011 number:1 day:18 month:11
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Complement Fixation Test High Responder Lung Tissue Sample Specific Antibody Response Herd Level
isfreeaccess_bool	false
container_title	BMC veterinary research
authorswithroles_txt_mv	Schubert, Evelyn @@aut@@ Sachse, Konrad @@aut@@ Jores, Jörg @@aut@@ Heller, Martin @@aut@@
publishDateDaySort_date	2011-11-18T00:00:00Z
hierarchy_top_id	489256538
id	SPR028371720
language_de	englisch
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author	Schubert, Evelyn
spellingShingle	Schubert, Evelyn misc Complement Fixation Test misc High Responder misc Lung Tissue Sample misc Specific Antibody Response misc Herd Level Serological testing of cattle experimentally infected with Mycoplasmamycoides subsp. mycoides Small Colony using four different tests reveals a variety of seroconversion patterns
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topic_title	Serological testing of cattle experimentally infected with Mycoplasmamycoides subsp. mycoides Small Colony using four different tests reveals a variety of seroconversion patterns Complement Fixation Test (dpeaa)DE-He213 High Responder (dpeaa)DE-He213 Lung Tissue Sample (dpeaa)DE-He213 Specific Antibody Response (dpeaa)DE-He213 Herd Level (dpeaa)DE-He213
topic	misc Complement Fixation Test misc High Responder misc Lung Tissue Sample misc Specific Antibody Response misc Herd Level
topic_unstemmed	misc Complement Fixation Test misc High Responder misc Lung Tissue Sample misc Specific Antibody Response misc Herd Level
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title	Serological testing of cattle experimentally infected with Mycoplasmamycoides subsp. mycoides Small Colony using four different tests reveals a variety of seroconversion patterns
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title_full	Serological testing of cattle experimentally infected with Mycoplasmamycoides subsp. mycoides Small Colony using four different tests reveals a variety of seroconversion patterns
author_sort	Schubert, Evelyn
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author_browse	Schubert, Evelyn Sachse, Konrad Jores, Jörg Heller, Martin
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title_sort	serological testing of cattle experimentally infected with mycoplasmamycoides subsp. mycoides small colony using four different tests reveals a variety of seroconversion patterns
title_auth	Serological testing of cattle experimentally infected with Mycoplasmamycoides subsp. mycoides Small Colony using four different tests reveals a variety of seroconversion patterns
abstract	Background To study the specific antibody response to infection with Mycoplasma mycoides subsp. mycoides Small Colony (MmmSC), the agent of Contagious Bovine Pleuropneumonia (CBPP), we examined three panels of sera collected during three experimental infection trials in African cattle. The methods used included an in-house complement fixation test (CFT), a commercially available CFT, a competitive antibody ELISA (cELISA) and the immunoblotting test (IBT). In addition, lung tissue samples were examined by culture. Results A total of 89% (51/59) of all experimentally infected animals tested positive on at least one of the serological tests throughout the trial. The specific antibody titres to the MmmSC infection became positive first by CFT (6 to 9 days post infection [dpi]), followed by IBT (9 to 13 dpi) and cELISA (13 to 16 dpi). Individual animals were found to display remarkably distinct seroconversion patterns, which allowed their classification into i) early high responders, ii) late high responders, and iii) low responders. In accordance with other studies, none of the present serological tests was capable of detecting all CBPP infected animals. Conclusion Comparison of the assays' performance in terms of sensitivity and specificity raises serious questions as to their reliability for identification of infected individuals in the field. In view of these limitations, a combination of CFT and cELISA can markedly improve CBPP diagnosis at single-animal level. © Schubert et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (
abstractGer	Background To study the specific antibody response to infection with Mycoplasma mycoides subsp. mycoides Small Colony (MmmSC), the agent of Contagious Bovine Pleuropneumonia (CBPP), we examined three panels of sera collected during three experimental infection trials in African cattle. The methods used included an in-house complement fixation test (CFT), a commercially available CFT, a competitive antibody ELISA (cELISA) and the immunoblotting test (IBT). In addition, lung tissue samples were examined by culture. Results A total of 89% (51/59) of all experimentally infected animals tested positive on at least one of the serological tests throughout the trial. The specific antibody titres to the MmmSC infection became positive first by CFT (6 to 9 days post infection [dpi]), followed by IBT (9 to 13 dpi) and cELISA (13 to 16 dpi). Individual animals were found to display remarkably distinct seroconversion patterns, which allowed their classification into i) early high responders, ii) late high responders, and iii) low responders. In accordance with other studies, none of the present serological tests was capable of detecting all CBPP infected animals. Conclusion Comparison of the assays' performance in terms of sensitivity and specificity raises serious questions as to their reliability for identification of infected individuals in the field. In view of these limitations, a combination of CFT and cELISA can markedly improve CBPP diagnosis at single-animal level. © Schubert et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (
abstract_unstemmed	Background To study the specific antibody response to infection with Mycoplasma mycoides subsp. mycoides Small Colony (MmmSC), the agent of Contagious Bovine Pleuropneumonia (CBPP), we examined three panels of sera collected during three experimental infection trials in African cattle. The methods used included an in-house complement fixation test (CFT), a commercially available CFT, a competitive antibody ELISA (cELISA) and the immunoblotting test (IBT). In addition, lung tissue samples were examined by culture. Results A total of 89% (51/59) of all experimentally infected animals tested positive on at least one of the serological tests throughout the trial. The specific antibody titres to the MmmSC infection became positive first by CFT (6 to 9 days post infection [dpi]), followed by IBT (9 to 13 dpi) and cELISA (13 to 16 dpi). Individual animals were found to display remarkably distinct seroconversion patterns, which allowed their classification into i) early high responders, ii) late high responders, and iii) low responders. In accordance with other studies, none of the present serological tests was capable of detecting all CBPP infected animals. Conclusion Comparison of the assays' performance in terms of sensitivity and specificity raises serious questions as to their reliability for identification of infected individuals in the field. In view of these limitations, a combination of CFT and cELISA can markedly improve CBPP diagnosis at single-animal level. © Schubert et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (
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title_short	Serological testing of cattle experimentally infected with Mycoplasmamycoides subsp. mycoides Small Colony using four different tests reveals a variety of seroconversion patterns
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Serological testing of cattle experimentally infected with Mycoplasmamycoides subsp. mycoides Small Colony using four different tests reveals a variety of seroconversion patterns

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