Pro-inflammatory mediators and neutrophils are increased in synovial fluid from heifers with acute ruminal acidosis

Background Acute ruminal acidosis (ARA) is a metabolic disease of cattle characterized by an aseptic synovitis. ARA is the result of an increased intake of highly fermentable carbohydrates that frequently occurs in dairy cattle subjected to high production requirements. In human joint diseases such as rheumatoid arthritis and gout, several pro-inflammatory molecules are increased in the synovial fluid, including cytokines, prostaglandin $ E_{2} $ ($ PGE_{2} $), metalloproteinases, and neutrophil extracellular traps (NETs). The aim of this study was to identify the presence of proinflammatory mediators and neutrophils in the synovial fluid of heifers with ARA, induced by an oligofructose overload. Five heifers were challenged with an oligofructose overload (13 g/kg BW) dissolved in water. As a control, a similar vehicle volume was used in four heifers. Synovial fluid samples were collected from the tarso-crural joint and $ PGE_{2} $, IL-6, IL-1β, ATP, lactate dehydrogenase (LDH), albumin, glucose, matrix metalloproteinase-9 (MMP-9), cellular free DNA, NETs, and serpin B1 were analyzed at 0, 9, and 24 h post treatment. Results At 9 h post oligofructose overload, an increase of IL-1β, IL-6, $ PGE_{2} $, serpin B1 and LDH was detected in the joints when compared to the control group. At 24 h, the synovial fluid was yellowish, viscous, turbid, and contained abundant neutrophils. An increase of DNA-backbone-like traps, histone 3 ($ H_{3} $cit), aggregated neutrophil extracellular traps (aggNETs), and serpin B1 were observed 24 h post treatment. Furthermore, albumins, LDH, ATP, MMP-9, IL-6, and IL-1β were increased after 24 h. Conclusions The overall results indicate that IL-1β, IL-6 and $ PGE_{2} $, were the earliest proinflammatory parameters that increased in the synovial fluid of animals with ARA. Furthermore, the most sever inflammatory response in the joint was observed after 24 h and could be associated with a massive presence of neutrophils and release of aggNETs. Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Hidalgo, Alejandra I. [verfasserIn] Carretta, María D. Alarcón, Pablo Manosalva, Carolina Müller, Ananda Navarro, Max Hidalgo, María A. Kaehne, Thilo Taubert, Anja Hermosilla, Carlos R. Burgos, Rafael A.

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2019

Schlagwörter:	Acute ruminal acidosis Synovial fluid Synovitis Cytokine Neutrophil extracellular traps Prostaglandin E

Anmerkung:	© The Author(s). 2019

Übergeordnetes Werk:	Enthalten in: BMC veterinary research - London : BioMed Central, 2005, 15(2019), 1 vom: 03. Juli
Übergeordnetes Werk:	volume:15 ; year:2019 ; number:1 ; day:03 ; month:07

Links:	Volltext

DOI / URN:	10.1186/s12917-019-1974-x

Katalog-ID:	SPR028399552

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100	1		\|a Hidalgo, Alejandra I. \|e verfasserin \|4 aut
245	1	0	\|a Pro-inflammatory mediators and neutrophils are increased in synovial fluid from heifers with acute ruminal acidosis
264		1	\|c 2019
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337			\|a Computermedien \|b c \|2 rdamedia
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520			\|a Background Acute ruminal acidosis (ARA) is a metabolic disease of cattle characterized by an aseptic synovitis. ARA is the result of an increased intake of highly fermentable carbohydrates that frequently occurs in dairy cattle subjected to high production requirements. In human joint diseases such as rheumatoid arthritis and gout, several pro-inflammatory molecules are increased in the synovial fluid, including cytokines, prostaglandin $ E_{2} $ ($ PGE_{2} $), metalloproteinases, and neutrophil extracellular traps (NETs). The aim of this study was to identify the presence of proinflammatory mediators and neutrophils in the synovial fluid of heifers with ARA, induced by an oligofructose overload. Five heifers were challenged with an oligofructose overload (13 g/kg BW) dissolved in water. As a control, a similar vehicle volume was used in four heifers. Synovial fluid samples were collected from the tarso-crural joint and $ PGE_{2} $, IL-6, IL-1β, ATP, lactate dehydrogenase (LDH), albumin, glucose, matrix metalloproteinase-9 (MMP-9), cellular free DNA, NETs, and serpin B1 were analyzed at 0, 9, and 24 h post treatment. Results At 9 h post oligofructose overload, an increase of IL-1β, IL-6, $ PGE_{2} $, serpin B1 and LDH was detected in the joints when compared to the control group. At 24 h, the synovial fluid was yellowish, viscous, turbid, and contained abundant neutrophils. An increase of DNA-backbone-like traps, histone 3 ($ H_{3} $cit), aggregated neutrophil extracellular traps (aggNETs), and serpin B1 were observed 24 h post treatment. Furthermore, albumins, LDH, ATP, MMP-9, IL-6, and IL-1β were increased after 24 h. Conclusions The overall results indicate that IL-1β, IL-6 and $ PGE_{2} $, were the earliest proinflammatory parameters that increased in the synovial fluid of animals with ARA. Furthermore, the most sever inflammatory response in the joint was observed after 24 h and could be associated with a massive presence of neutrophils and release of aggNETs.
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700	1		\|a Alarcón, Pablo \|4 aut
700	1		\|a Manosalva, Carolina \|4 aut
700	1		\|a Müller, Ananda \|4 aut
700	1		\|a Navarro, Max \|4 aut
700	1		\|a Hidalgo, María A. \|4 aut
700	1		\|a Kaehne, Thilo \|4 aut
700	1		\|a Taubert, Anja \|4 aut
700	1		\|a Hermosilla, Carlos R. \|4 aut
700	1		\|a Burgos, Rafael A. \|0 (orcid)0000-0002-8182-2502 \|4 aut
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allfields	10.1186/s12917-019-1974-x doi (DE-627)SPR028399552 (SPR)s12917-019-1974-x-e DE-627 ger DE-627 rakwb eng Hidalgo, Alejandra I. verfasserin aut Pro-inflammatory mediators and neutrophils are increased in synovial fluid from heifers with acute ruminal acidosis 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2019 Background Acute ruminal acidosis (ARA) is a metabolic disease of cattle characterized by an aseptic synovitis. ARA is the result of an increased intake of highly fermentable carbohydrates that frequently occurs in dairy cattle subjected to high production requirements. In human joint diseases such as rheumatoid arthritis and gout, several pro-inflammatory molecules are increased in the synovial fluid, including cytokines, prostaglandin $ E_{2} $ ($ PGE_{2} $), metalloproteinases, and neutrophil extracellular traps (NETs). The aim of this study was to identify the presence of proinflammatory mediators and neutrophils in the synovial fluid of heifers with ARA, induced by an oligofructose overload. Five heifers were challenged with an oligofructose overload (13 g/kg BW) dissolved in water. As a control, a similar vehicle volume was used in four heifers. Synovial fluid samples were collected from the tarso-crural joint and $ PGE_{2} $, IL-6, IL-1β, ATP, lactate dehydrogenase (LDH), albumin, glucose, matrix metalloproteinase-9 (MMP-9), cellular free DNA, NETs, and serpin B1 were analyzed at 0, 9, and 24 h post treatment. Results At 9 h post oligofructose overload, an increase of IL-1β, IL-6, $ PGE_{2} $, serpin B1 and LDH was detected in the joints when compared to the control group. At 24 h, the synovial fluid was yellowish, viscous, turbid, and contained abundant neutrophils. An increase of DNA-backbone-like traps, histone 3 ($ H_{3} $cit), aggregated neutrophil extracellular traps (aggNETs), and serpin B1 were observed 24 h post treatment. Furthermore, albumins, LDH, ATP, MMP-9, IL-6, and IL-1β were increased after 24 h. Conclusions The overall results indicate that IL-1β, IL-6 and $ PGE_{2} $, were the earliest proinflammatory parameters that increased in the synovial fluid of animals with ARA. Furthermore, the most sever inflammatory response in the joint was observed after 24 h and could be associated with a massive presence of neutrophils and release of aggNETs. Acute ruminal acidosis (dpeaa)DE-He213 Synovial fluid (dpeaa)DE-He213 Synovitis (dpeaa)DE-He213 Cytokine (dpeaa)DE-He213 Neutrophil extracellular traps (dpeaa)DE-He213 Prostaglandin E (dpeaa)DE-He213 Carretta, María D. aut Alarcón, Pablo aut Manosalva, Carolina aut Müller, Ananda aut Navarro, Max aut Hidalgo, María A. aut Kaehne, Thilo aut Taubert, Anja aut Hermosilla, Carlos R. aut Burgos, Rafael A. (orcid)0000-0002-8182-2502 aut Enthalten in BMC veterinary research London : BioMed Central, 2005 15(2019), 1 vom: 03. Juli (DE-627)489256538 (DE-600)2191675-5 1746-6148 nnns volume:15 year:2019 number:1 day:03 month:07 https://dx.doi.org/10.1186/s12917-019-1974-x kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2019 1 03 07
spelling	10.1186/s12917-019-1974-x doi (DE-627)SPR028399552 (SPR)s12917-019-1974-x-e DE-627 ger DE-627 rakwb eng Hidalgo, Alejandra I. verfasserin aut Pro-inflammatory mediators and neutrophils are increased in synovial fluid from heifers with acute ruminal acidosis 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2019 Background Acute ruminal acidosis (ARA) is a metabolic disease of cattle characterized by an aseptic synovitis. ARA is the result of an increased intake of highly fermentable carbohydrates that frequently occurs in dairy cattle subjected to high production requirements. In human joint diseases such as rheumatoid arthritis and gout, several pro-inflammatory molecules are increased in the synovial fluid, including cytokines, prostaglandin $ E_{2} $ ($ PGE_{2} $), metalloproteinases, and neutrophil extracellular traps (NETs). The aim of this study was to identify the presence of proinflammatory mediators and neutrophils in the synovial fluid of heifers with ARA, induced by an oligofructose overload. Five heifers were challenged with an oligofructose overload (13 g/kg BW) dissolved in water. As a control, a similar vehicle volume was used in four heifers. Synovial fluid samples were collected from the tarso-crural joint and $ PGE_{2} $, IL-6, IL-1β, ATP, lactate dehydrogenase (LDH), albumin, glucose, matrix metalloproteinase-9 (MMP-9), cellular free DNA, NETs, and serpin B1 were analyzed at 0, 9, and 24 h post treatment. Results At 9 h post oligofructose overload, an increase of IL-1β, IL-6, $ PGE_{2} $, serpin B1 and LDH was detected in the joints when compared to the control group. At 24 h, the synovial fluid was yellowish, viscous, turbid, and contained abundant neutrophils. An increase of DNA-backbone-like traps, histone 3 ($ H_{3} $cit), aggregated neutrophil extracellular traps (aggNETs), and serpin B1 were observed 24 h post treatment. Furthermore, albumins, LDH, ATP, MMP-9, IL-6, and IL-1β were increased after 24 h. Conclusions The overall results indicate that IL-1β, IL-6 and $ PGE_{2} $, were the earliest proinflammatory parameters that increased in the synovial fluid of animals with ARA. Furthermore, the most sever inflammatory response in the joint was observed after 24 h and could be associated with a massive presence of neutrophils and release of aggNETs. Acute ruminal acidosis (dpeaa)DE-He213 Synovial fluid (dpeaa)DE-He213 Synovitis (dpeaa)DE-He213 Cytokine (dpeaa)DE-He213 Neutrophil extracellular traps (dpeaa)DE-He213 Prostaglandin E (dpeaa)DE-He213 Carretta, María D. aut Alarcón, Pablo aut Manosalva, Carolina aut Müller, Ananda aut Navarro, Max aut Hidalgo, María A. aut Kaehne, Thilo aut Taubert, Anja aut Hermosilla, Carlos R. aut Burgos, Rafael A. (orcid)0000-0002-8182-2502 aut Enthalten in BMC veterinary research London : BioMed Central, 2005 15(2019), 1 vom: 03. Juli (DE-627)489256538 (DE-600)2191675-5 1746-6148 nnns volume:15 year:2019 number:1 day:03 month:07 https://dx.doi.org/10.1186/s12917-019-1974-x kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2019 1 03 07
allfields_unstemmed	10.1186/s12917-019-1974-x doi (DE-627)SPR028399552 (SPR)s12917-019-1974-x-e DE-627 ger DE-627 rakwb eng Hidalgo, Alejandra I. verfasserin aut Pro-inflammatory mediators and neutrophils are increased in synovial fluid from heifers with acute ruminal acidosis 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2019 Background Acute ruminal acidosis (ARA) is a metabolic disease of cattle characterized by an aseptic synovitis. ARA is the result of an increased intake of highly fermentable carbohydrates that frequently occurs in dairy cattle subjected to high production requirements. In human joint diseases such as rheumatoid arthritis and gout, several pro-inflammatory molecules are increased in the synovial fluid, including cytokines, prostaglandin $ E_{2} $ ($ PGE_{2} $), metalloproteinases, and neutrophil extracellular traps (NETs). The aim of this study was to identify the presence of proinflammatory mediators and neutrophils in the synovial fluid of heifers with ARA, induced by an oligofructose overload. Five heifers were challenged with an oligofructose overload (13 g/kg BW) dissolved in water. As a control, a similar vehicle volume was used in four heifers. Synovial fluid samples were collected from the tarso-crural joint and $ PGE_{2} $, IL-6, IL-1β, ATP, lactate dehydrogenase (LDH), albumin, glucose, matrix metalloproteinase-9 (MMP-9), cellular free DNA, NETs, and serpin B1 were analyzed at 0, 9, and 24 h post treatment. Results At 9 h post oligofructose overload, an increase of IL-1β, IL-6, $ PGE_{2} $, serpin B1 and LDH was detected in the joints when compared to the control group. At 24 h, the synovial fluid was yellowish, viscous, turbid, and contained abundant neutrophils. An increase of DNA-backbone-like traps, histone 3 ($ H_{3} $cit), aggregated neutrophil extracellular traps (aggNETs), and serpin B1 were observed 24 h post treatment. Furthermore, albumins, LDH, ATP, MMP-9, IL-6, and IL-1β were increased after 24 h. Conclusions The overall results indicate that IL-1β, IL-6 and $ PGE_{2} $, were the earliest proinflammatory parameters that increased in the synovial fluid of animals with ARA. Furthermore, the most sever inflammatory response in the joint was observed after 24 h and could be associated with a massive presence of neutrophils and release of aggNETs. Acute ruminal acidosis (dpeaa)DE-He213 Synovial fluid (dpeaa)DE-He213 Synovitis (dpeaa)DE-He213 Cytokine (dpeaa)DE-He213 Neutrophil extracellular traps (dpeaa)DE-He213 Prostaglandin E (dpeaa)DE-He213 Carretta, María D. aut Alarcón, Pablo aut Manosalva, Carolina aut Müller, Ananda aut Navarro, Max aut Hidalgo, María A. aut Kaehne, Thilo aut Taubert, Anja aut Hermosilla, Carlos R. aut Burgos, Rafael A. (orcid)0000-0002-8182-2502 aut Enthalten in BMC veterinary research London : BioMed Central, 2005 15(2019), 1 vom: 03. Juli (DE-627)489256538 (DE-600)2191675-5 1746-6148 nnns volume:15 year:2019 number:1 day:03 month:07 https://dx.doi.org/10.1186/s12917-019-1974-x kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2019 1 03 07
allfieldsGer	10.1186/s12917-019-1974-x doi (DE-627)SPR028399552 (SPR)s12917-019-1974-x-e DE-627 ger DE-627 rakwb eng Hidalgo, Alejandra I. verfasserin aut Pro-inflammatory mediators and neutrophils are increased in synovial fluid from heifers with acute ruminal acidosis 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2019 Background Acute ruminal acidosis (ARA) is a metabolic disease of cattle characterized by an aseptic synovitis. ARA is the result of an increased intake of highly fermentable carbohydrates that frequently occurs in dairy cattle subjected to high production requirements. In human joint diseases such as rheumatoid arthritis and gout, several pro-inflammatory molecules are increased in the synovial fluid, including cytokines, prostaglandin $ E_{2} $ ($ PGE_{2} $), metalloproteinases, and neutrophil extracellular traps (NETs). The aim of this study was to identify the presence of proinflammatory mediators and neutrophils in the synovial fluid of heifers with ARA, induced by an oligofructose overload. Five heifers were challenged with an oligofructose overload (13 g/kg BW) dissolved in water. As a control, a similar vehicle volume was used in four heifers. Synovial fluid samples were collected from the tarso-crural joint and $ PGE_{2} $, IL-6, IL-1β, ATP, lactate dehydrogenase (LDH), albumin, glucose, matrix metalloproteinase-9 (MMP-9), cellular free DNA, NETs, and serpin B1 were analyzed at 0, 9, and 24 h post treatment. Results At 9 h post oligofructose overload, an increase of IL-1β, IL-6, $ PGE_{2} $, serpin B1 and LDH was detected in the joints when compared to the control group. At 24 h, the synovial fluid was yellowish, viscous, turbid, and contained abundant neutrophils. An increase of DNA-backbone-like traps, histone 3 ($ H_{3} $cit), aggregated neutrophil extracellular traps (aggNETs), and serpin B1 were observed 24 h post treatment. Furthermore, albumins, LDH, ATP, MMP-9, IL-6, and IL-1β were increased after 24 h. Conclusions The overall results indicate that IL-1β, IL-6 and $ PGE_{2} $, were the earliest proinflammatory parameters that increased in the synovial fluid of animals with ARA. Furthermore, the most sever inflammatory response in the joint was observed after 24 h and could be associated with a massive presence of neutrophils and release of aggNETs. Acute ruminal acidosis (dpeaa)DE-He213 Synovial fluid (dpeaa)DE-He213 Synovitis (dpeaa)DE-He213 Cytokine (dpeaa)DE-He213 Neutrophil extracellular traps (dpeaa)DE-He213 Prostaglandin E (dpeaa)DE-He213 Carretta, María D. aut Alarcón, Pablo aut Manosalva, Carolina aut Müller, Ananda aut Navarro, Max aut Hidalgo, María A. aut Kaehne, Thilo aut Taubert, Anja aut Hermosilla, Carlos R. aut Burgos, Rafael A. (orcid)0000-0002-8182-2502 aut Enthalten in BMC veterinary research London : BioMed Central, 2005 15(2019), 1 vom: 03. Juli (DE-627)489256538 (DE-600)2191675-5 1746-6148 nnns volume:15 year:2019 number:1 day:03 month:07 https://dx.doi.org/10.1186/s12917-019-1974-x kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2019 1 03 07
allfieldsSound	10.1186/s12917-019-1974-x doi (DE-627)SPR028399552 (SPR)s12917-019-1974-x-e DE-627 ger DE-627 rakwb eng Hidalgo, Alejandra I. verfasserin aut Pro-inflammatory mediators and neutrophils are increased in synovial fluid from heifers with acute ruminal acidosis 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2019 Background Acute ruminal acidosis (ARA) is a metabolic disease of cattle characterized by an aseptic synovitis. ARA is the result of an increased intake of highly fermentable carbohydrates that frequently occurs in dairy cattle subjected to high production requirements. In human joint diseases such as rheumatoid arthritis and gout, several pro-inflammatory molecules are increased in the synovial fluid, including cytokines, prostaglandin $ E_{2} $ ($ PGE_{2} $), metalloproteinases, and neutrophil extracellular traps (NETs). The aim of this study was to identify the presence of proinflammatory mediators and neutrophils in the synovial fluid of heifers with ARA, induced by an oligofructose overload. Five heifers were challenged with an oligofructose overload (13 g/kg BW) dissolved in water. As a control, a similar vehicle volume was used in four heifers. Synovial fluid samples were collected from the tarso-crural joint and $ PGE_{2} $, IL-6, IL-1β, ATP, lactate dehydrogenase (LDH), albumin, glucose, matrix metalloproteinase-9 (MMP-9), cellular free DNA, NETs, and serpin B1 were analyzed at 0, 9, and 24 h post treatment. Results At 9 h post oligofructose overload, an increase of IL-1β, IL-6, $ PGE_{2} $, serpin B1 and LDH was detected in the joints when compared to the control group. At 24 h, the synovial fluid was yellowish, viscous, turbid, and contained abundant neutrophils. An increase of DNA-backbone-like traps, histone 3 ($ H_{3} $cit), aggregated neutrophil extracellular traps (aggNETs), and serpin B1 were observed 24 h post treatment. Furthermore, albumins, LDH, ATP, MMP-9, IL-6, and IL-1β were increased after 24 h. Conclusions The overall results indicate that IL-1β, IL-6 and $ PGE_{2} $, were the earliest proinflammatory parameters that increased in the synovial fluid of animals with ARA. Furthermore, the most sever inflammatory response in the joint was observed after 24 h and could be associated with a massive presence of neutrophils and release of aggNETs. Acute ruminal acidosis (dpeaa)DE-He213 Synovial fluid (dpeaa)DE-He213 Synovitis (dpeaa)DE-He213 Cytokine (dpeaa)DE-He213 Neutrophil extracellular traps (dpeaa)DE-He213 Prostaglandin E (dpeaa)DE-He213 Carretta, María D. aut Alarcón, Pablo aut Manosalva, Carolina aut Müller, Ananda aut Navarro, Max aut Hidalgo, María A. aut Kaehne, Thilo aut Taubert, Anja aut Hermosilla, Carlos R. aut Burgos, Rafael A. (orcid)0000-0002-8182-2502 aut Enthalten in BMC veterinary research London : BioMed Central, 2005 15(2019), 1 vom: 03. Juli (DE-627)489256538 (DE-600)2191675-5 1746-6148 nnns volume:15 year:2019 number:1 day:03 month:07 https://dx.doi.org/10.1186/s12917-019-1974-x kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2019 1 03 07
language	English
source	Enthalten in BMC veterinary research 15(2019), 1 vom: 03. Juli volume:15 year:2019 number:1 day:03 month:07
sourceStr	Enthalten in BMC veterinary research 15(2019), 1 vom: 03. Juli volume:15 year:2019 number:1 day:03 month:07
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Acute ruminal acidosis Synovial fluid Synovitis Cytokine Neutrophil extracellular traps Prostaglandin E
isfreeaccess_bool	true
container_title	BMC veterinary research
authorswithroles_txt_mv	Hidalgo, Alejandra I. @@aut@@ Carretta, María D. @@aut@@ Alarcón, Pablo @@aut@@ Manosalva, Carolina @@aut@@ Müller, Ananda @@aut@@ Navarro, Max @@aut@@ Hidalgo, María A. @@aut@@ Kaehne, Thilo @@aut@@ Taubert, Anja @@aut@@ Hermosilla, Carlos R. @@aut@@ Burgos, Rafael A. @@aut@@
publishDateDaySort_date	2019-07-03T00:00:00Z
hierarchy_top_id	489256538
id	SPR028399552
language_de	englisch
fullrecord	<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">SPR028399552</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230519154901.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">201007s2019 xx \|\|\|\|\|o 00\| \|\|eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1186/s12917-019-1974-x</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)SPR028399552</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(SPR)s12917-019-1974-x-e</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Hidalgo, Alejandra I.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Pro-inflammatory mediators and neutrophils are increased in synovial fluid from heifers with acute ruminal acidosis</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2019</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">© The Author(s). 2019</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Background Acute ruminal acidosis (ARA) is a metabolic disease of cattle characterized by an aseptic synovitis. ARA is the result of an increased intake of highly fermentable carbohydrates that frequently occurs in dairy cattle subjected to high production requirements. In human joint diseases such as rheumatoid arthritis and gout, several pro-inflammatory molecules are increased in the synovial fluid, including cytokines, prostaglandin $ E_{2} $ ($ PGE_{2} $), metalloproteinases, and neutrophil extracellular traps (NETs). The aim of this study was to identify the presence of proinflammatory mediators and neutrophils in the synovial fluid of heifers with ARA, induced by an oligofructose overload. Five heifers were challenged with an oligofructose overload (13 g/kg BW) dissolved in water. As a control, a similar vehicle volume was used in four heifers. Synovial fluid samples were collected from the tarso-crural joint and $ PGE_{2} $, IL-6, IL-1β, ATP, lactate dehydrogenase (LDH), albumin, glucose, matrix metalloproteinase-9 (MMP-9), cellular free DNA, NETs, and serpin B1 were analyzed at 0, 9, and 24 h post treatment. Results At 9 h post oligofructose overload, an increase of IL-1β, IL-6, $ PGE_{2} $, serpin B1 and LDH was detected in the joints when compared to the control group. At 24 h, the synovial fluid was yellowish, viscous, turbid, and contained abundant neutrophils. An increase of DNA-backbone-like traps, histone 3 ($ H_{3} $cit), aggregated neutrophil extracellular traps (aggNETs), and serpin B1 were observed 24 h post treatment. Furthermore, albumins, LDH, ATP, MMP-9, IL-6, and IL-1β were increased after 24 h. Conclusions The overall results indicate that IL-1β, IL-6 and $ PGE_{2} $, were the earliest proinflammatory parameters that increased in the synovial fluid of animals with ARA. 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author	Hidalgo, Alejandra I.
spellingShingle	Hidalgo, Alejandra I. misc Acute ruminal acidosis misc Synovial fluid misc Synovitis misc Cytokine misc Neutrophil extracellular traps misc Prostaglandin E Pro-inflammatory mediators and neutrophils are increased in synovial fluid from heifers with acute ruminal acidosis
authorStr	Hidalgo, Alejandra I.
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topic_title	Pro-inflammatory mediators and neutrophils are increased in synovial fluid from heifers with acute ruminal acidosis Acute ruminal acidosis (dpeaa)DE-He213 Synovial fluid (dpeaa)DE-He213 Synovitis (dpeaa)DE-He213 Cytokine (dpeaa)DE-He213 Neutrophil extracellular traps (dpeaa)DE-He213 Prostaglandin E (dpeaa)DE-He213
topic	misc Acute ruminal acidosis misc Synovial fluid misc Synovitis misc Cytokine misc Neutrophil extracellular traps misc Prostaglandin E
topic_unstemmed	misc Acute ruminal acidosis misc Synovial fluid misc Synovitis misc Cytokine misc Neutrophil extracellular traps misc Prostaglandin E
topic_browse	misc Acute ruminal acidosis misc Synovial fluid misc Synovitis misc Cytokine misc Neutrophil extracellular traps misc Prostaglandin E
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title	Pro-inflammatory mediators and neutrophils are increased in synovial fluid from heifers with acute ruminal acidosis
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title_full	Pro-inflammatory mediators and neutrophils are increased in synovial fluid from heifers with acute ruminal acidosis
author_sort	Hidalgo, Alejandra I.
journal	BMC veterinary research
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author_browse	Hidalgo, Alejandra I. Carretta, María D. Alarcón, Pablo Manosalva, Carolina Müller, Ananda Navarro, Max Hidalgo, María A. Kaehne, Thilo Taubert, Anja Hermosilla, Carlos R. Burgos, Rafael A.
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author-letter	Hidalgo, Alejandra I.
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title_sort	pro-inflammatory mediators and neutrophils are increased in synovial fluid from heifers with acute ruminal acidosis
title_auth	Pro-inflammatory mediators and neutrophils are increased in synovial fluid from heifers with acute ruminal acidosis
abstract	Background Acute ruminal acidosis (ARA) is a metabolic disease of cattle characterized by an aseptic synovitis. ARA is the result of an increased intake of highly fermentable carbohydrates that frequently occurs in dairy cattle subjected to high production requirements. In human joint diseases such as rheumatoid arthritis and gout, several pro-inflammatory molecules are increased in the synovial fluid, including cytokines, prostaglandin $ E_{2} $ ($ PGE_{2} $), metalloproteinases, and neutrophil extracellular traps (NETs). The aim of this study was to identify the presence of proinflammatory mediators and neutrophils in the synovial fluid of heifers with ARA, induced by an oligofructose overload. Five heifers were challenged with an oligofructose overload (13 g/kg BW) dissolved in water. As a control, a similar vehicle volume was used in four heifers. Synovial fluid samples were collected from the tarso-crural joint and $ PGE_{2} $, IL-6, IL-1β, ATP, lactate dehydrogenase (LDH), albumin, glucose, matrix metalloproteinase-9 (MMP-9), cellular free DNA, NETs, and serpin B1 were analyzed at 0, 9, and 24 h post treatment. Results At 9 h post oligofructose overload, an increase of IL-1β, IL-6, $ PGE_{2} $, serpin B1 and LDH was detected in the joints when compared to the control group. At 24 h, the synovial fluid was yellowish, viscous, turbid, and contained abundant neutrophils. An increase of DNA-backbone-like traps, histone 3 ($ H_{3} $cit), aggregated neutrophil extracellular traps (aggNETs), and serpin B1 were observed 24 h post treatment. Furthermore, albumins, LDH, ATP, MMP-9, IL-6, and IL-1β were increased after 24 h. Conclusions The overall results indicate that IL-1β, IL-6 and $ PGE_{2} $, were the earliest proinflammatory parameters that increased in the synovial fluid of animals with ARA. Furthermore, the most sever inflammatory response in the joint was observed after 24 h and could be associated with a massive presence of neutrophils and release of aggNETs. © The Author(s). 2019
abstractGer	Background Acute ruminal acidosis (ARA) is a metabolic disease of cattle characterized by an aseptic synovitis. ARA is the result of an increased intake of highly fermentable carbohydrates that frequently occurs in dairy cattle subjected to high production requirements. In human joint diseases such as rheumatoid arthritis and gout, several pro-inflammatory molecules are increased in the synovial fluid, including cytokines, prostaglandin $ E_{2} $ ($ PGE_{2} $), metalloproteinases, and neutrophil extracellular traps (NETs). The aim of this study was to identify the presence of proinflammatory mediators and neutrophils in the synovial fluid of heifers with ARA, induced by an oligofructose overload. Five heifers were challenged with an oligofructose overload (13 g/kg BW) dissolved in water. As a control, a similar vehicle volume was used in four heifers. Synovial fluid samples were collected from the tarso-crural joint and $ PGE_{2} $, IL-6, IL-1β, ATP, lactate dehydrogenase (LDH), albumin, glucose, matrix metalloproteinase-9 (MMP-9), cellular free DNA, NETs, and serpin B1 were analyzed at 0, 9, and 24 h post treatment. Results At 9 h post oligofructose overload, an increase of IL-1β, IL-6, $ PGE_{2} $, serpin B1 and LDH was detected in the joints when compared to the control group. At 24 h, the synovial fluid was yellowish, viscous, turbid, and contained abundant neutrophils. An increase of DNA-backbone-like traps, histone 3 ($ H_{3} $cit), aggregated neutrophil extracellular traps (aggNETs), and serpin B1 were observed 24 h post treatment. Furthermore, albumins, LDH, ATP, MMP-9, IL-6, and IL-1β were increased after 24 h. Conclusions The overall results indicate that IL-1β, IL-6 and $ PGE_{2} $, were the earliest proinflammatory parameters that increased in the synovial fluid of animals with ARA. Furthermore, the most sever inflammatory response in the joint was observed after 24 h and could be associated with a massive presence of neutrophils and release of aggNETs. © The Author(s). 2019
abstract_unstemmed	Background Acute ruminal acidosis (ARA) is a metabolic disease of cattle characterized by an aseptic synovitis. ARA is the result of an increased intake of highly fermentable carbohydrates that frequently occurs in dairy cattle subjected to high production requirements. In human joint diseases such as rheumatoid arthritis and gout, several pro-inflammatory molecules are increased in the synovial fluid, including cytokines, prostaglandin $ E_{2} $ ($ PGE_{2} $), metalloproteinases, and neutrophil extracellular traps (NETs). The aim of this study was to identify the presence of proinflammatory mediators and neutrophils in the synovial fluid of heifers with ARA, induced by an oligofructose overload. Five heifers were challenged with an oligofructose overload (13 g/kg BW) dissolved in water. As a control, a similar vehicle volume was used in four heifers. Synovial fluid samples were collected from the tarso-crural joint and $ PGE_{2} $, IL-6, IL-1β, ATP, lactate dehydrogenase (LDH), albumin, glucose, matrix metalloproteinase-9 (MMP-9), cellular free DNA, NETs, and serpin B1 were analyzed at 0, 9, and 24 h post treatment. Results At 9 h post oligofructose overload, an increase of IL-1β, IL-6, $ PGE_{2} $, serpin B1 and LDH was detected in the joints when compared to the control group. At 24 h, the synovial fluid was yellowish, viscous, turbid, and contained abundant neutrophils. An increase of DNA-backbone-like traps, histone 3 ($ H_{3} $cit), aggregated neutrophil extracellular traps (aggNETs), and serpin B1 were observed 24 h post treatment. Furthermore, albumins, LDH, ATP, MMP-9, IL-6, and IL-1β were increased after 24 h. Conclusions The overall results indicate that IL-1β, IL-6 and $ PGE_{2} $, were the earliest proinflammatory parameters that increased in the synovial fluid of animals with ARA. Furthermore, the most sever inflammatory response in the joint was observed after 24 h and could be associated with a massive presence of neutrophils and release of aggNETs. © The Author(s). 2019
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title_short	Pro-inflammatory mediators and neutrophils are increased in synovial fluid from heifers with acute ruminal acidosis
url	https://dx.doi.org/10.1186/s12917-019-1974-x
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author2	Carretta, María D. Alarcón, Pablo Manosalva, Carolina Müller, Ananda Navarro, Max Hidalgo, María A. Kaehne, Thilo Taubert, Anja Hermosilla, Carlos R. Burgos, Rafael A.
author2Str	Carretta, María D. Alarcón, Pablo Manosalva, Carolina Müller, Ananda Navarro, Max Hidalgo, María A. Kaehne, Thilo Taubert, Anja Hermosilla, Carlos R. Burgos, Rafael A.
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ARA is the result of an increased intake of highly fermentable carbohydrates that frequently occurs in dairy cattle subjected to high production requirements. In human joint diseases such as rheumatoid arthritis and gout, several pro-inflammatory molecules are increased in the synovial fluid, including cytokines, prostaglandin $ E_{2} $ ($ PGE_{2} $), metalloproteinases, and neutrophil extracellular traps (NETs). The aim of this study was to identify the presence of proinflammatory mediators and neutrophils in the synovial fluid of heifers with ARA, induced by an oligofructose overload. Five heifers were challenged with an oligofructose overload (13 g/kg BW) dissolved in water. As a control, a similar vehicle volume was used in four heifers. Synovial fluid samples were collected from the tarso-crural joint and $ PGE_{2} $, IL-6, IL-1β, ATP, lactate dehydrogenase (LDH), albumin, glucose, matrix metalloproteinase-9 (MMP-9), cellular free DNA, NETs, and serpin B1 were analyzed at 0, 9, and 24 h post treatment. Results At 9 h post oligofructose overload, an increase of IL-1β, IL-6, $ PGE_{2} $, serpin B1 and LDH was detected in the joints when compared to the control group. At 24 h, the synovial fluid was yellowish, viscous, turbid, and contained abundant neutrophils. An increase of DNA-backbone-like traps, histone 3 ($ H_{3} $cit), aggregated neutrophil extracellular traps (aggNETs), and serpin B1 were observed 24 h post treatment. Furthermore, albumins, LDH, ATP, MMP-9, IL-6, and IL-1β were increased after 24 h. Conclusions The overall results indicate that IL-1β, IL-6 and $ PGE_{2} $, were the earliest proinflammatory parameters that increased in the synovial fluid of animals with ARA. 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Pro-inflammatory mediators and neutrophils are increased in synovial fluid from heifers with acute ruminal acidosis

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