Specific detection of foot-and-mouth disease serotype Asia 1 virus by carboxyl-magnetic beads conjugated with single-domain antibody

Background Immunomagnetic nanobead (IMNB) labeled with specific antibody, has been demonstrated to be useful for the capturing and detection of viruses. Results In this study, we developed an imunomagnetic bead based on carboxyl-magnetic beads (MNB) labeled with a single-domain antibody (sdAb) for c...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Yang, Shunli [verfasserIn] Yin, Shuanghui Shang, Youjun Wang, Di Ma, Weimin He, Jijun Guo, Jianhong Cai, Jianping Liu, Xiangtao

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2015

Schlagwörter:	Reverse Transcription Polymerase Chain Reaction Target Virus Immunomagnetic Bead Capture Capacity Reverse Transcription Polymerase Chain Reaction Assay

Anmerkung:	© Yang et al. 2015

Übergeordnetes Werk:	Enthalten in: BMC biotechnology - London : BioMed Central, 2001, 15(2015), 1 vom: 15. Sept.
Übergeordnetes Werk:	volume:15 ; year:2015 ; number:1 ; day:15 ; month:09

Links:	Volltext

DOI / URN:	10.1186/s12896-015-0201-5

Katalog-ID:	SPR028440412

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520			\|a Background Immunomagnetic nanobead (IMNB) labeled with specific antibody, has been demonstrated to be useful for the capturing and detection of viruses. Results In this study, we developed an imunomagnetic bead based on carboxyl-magnetic beads (MNB) labeled with a single-domain antibody (sdAb) for capturing foot-and-mouth disease (FMD) Asia 1 virus. After magnetic separation, complexes of MNB-sdAb-virus were detected with either a sandwich ELISA or QDs-C5 probe under a fluorescence microscope, and the complexes were used as templates for extraction of total RNA for amplification of the VP1 or 3D gene fragments using RT-PCR and real-time RT-PCR. The Asia 1 VLPs were efficiently captured through IMNB with a high binding rate of 5.09 μg of antigen/μl of bead suspension. Moreover, this method has been successfully used to capture Asia 1 antigen in synthetic samples. Conclusion Ultimately, a specific and highly sensitive capture FMDV Asia 1 tool has been established that has the potential to enhance the sensitivity and reliability when diagnosing FMDV Asia 1.
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700	1		\|a He, Jijun \|4 aut
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700	1		\|a Liu, Xiangtao \|4 aut
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912			\|a GBV_ILN_2031
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912			\|a GBV_ILN_2044
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912			\|a GBV_ILN_4324
912			\|a GBV_ILN_4325
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912			\|a GBV_ILN_4700
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Indexfelder

author_variant	s y sy s y sy y s ys d w dw w m wm j h jh j g jg j c jc x l xl
matchkey_str	article:14726750:2015----::pcfceetoofoadotdsaeeoyesavrsyabxlantcedcnu
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publishDate	2015
allfields	10.1186/s12896-015-0201-5 doi (DE-627)SPR028440412 (SPR)s12896-015-0201-5-e DE-627 ger DE-627 rakwb eng Yang, Shunli verfasserin aut Specific detection of foot-and-mouth disease serotype Asia 1 virus by carboxyl-magnetic beads conjugated with single-domain antibody 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Yang et al. 2015 Background Immunomagnetic nanobead (IMNB) labeled with specific antibody, has been demonstrated to be useful for the capturing and detection of viruses. Results In this study, we developed an imunomagnetic bead based on carboxyl-magnetic beads (MNB) labeled with a single-domain antibody (sdAb) for capturing foot-and-mouth disease (FMD) Asia 1 virus. After magnetic separation, complexes of MNB-sdAb-virus were detected with either a sandwich ELISA or QDs-C5 probe under a fluorescence microscope, and the complexes were used as templates for extraction of total RNA for amplification of the VP1 or 3D gene fragments using RT-PCR and real-time RT-PCR. The Asia 1 VLPs were efficiently captured through IMNB with a high binding rate of 5.09 μg of antigen/μl of bead suspension. Moreover, this method has been successfully used to capture Asia 1 antigen in synthetic samples. Conclusion Ultimately, a specific and highly sensitive capture FMDV Asia 1 tool has been established that has the potential to enhance the sensitivity and reliability when diagnosing FMDV Asia 1. Reverse Transcription Polymerase Chain Reaction (dpeaa)DE-He213 Target Virus (dpeaa)DE-He213 Immunomagnetic Bead (dpeaa)DE-He213 Capture Capacity (dpeaa)DE-He213 Reverse Transcription Polymerase Chain Reaction Assay (dpeaa)DE-He213 Yin, Shuanghui aut Shang, Youjun aut Wang, Di aut Ma, Weimin aut He, Jijun aut Guo, Jianhong aut Cai, Jianping aut Liu, Xiangtao aut Enthalten in BMC biotechnology London : BioMed Central, 2001 15(2015), 1 vom: 15. Sept. (DE-627)332164837 (DE-600)2052746-9 1472-6750 nnns volume:15 year:2015 number:1 day:15 month:09 https://dx.doi.org/10.1186/s12896-015-0201-5 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2119 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2015 1 15 09
spelling	10.1186/s12896-015-0201-5 doi (DE-627)SPR028440412 (SPR)s12896-015-0201-5-e DE-627 ger DE-627 rakwb eng Yang, Shunli verfasserin aut Specific detection of foot-and-mouth disease serotype Asia 1 virus by carboxyl-magnetic beads conjugated with single-domain antibody 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Yang et al. 2015 Background Immunomagnetic nanobead (IMNB) labeled with specific antibody, has been demonstrated to be useful for the capturing and detection of viruses. Results In this study, we developed an imunomagnetic bead based on carboxyl-magnetic beads (MNB) labeled with a single-domain antibody (sdAb) for capturing foot-and-mouth disease (FMD) Asia 1 virus. After magnetic separation, complexes of MNB-sdAb-virus were detected with either a sandwich ELISA or QDs-C5 probe under a fluorescence microscope, and the complexes were used as templates for extraction of total RNA for amplification of the VP1 or 3D gene fragments using RT-PCR and real-time RT-PCR. The Asia 1 VLPs were efficiently captured through IMNB with a high binding rate of 5.09 μg of antigen/μl of bead suspension. Moreover, this method has been successfully used to capture Asia 1 antigen in synthetic samples. Conclusion Ultimately, a specific and highly sensitive capture FMDV Asia 1 tool has been established that has the potential to enhance the sensitivity and reliability when diagnosing FMDV Asia 1. Reverse Transcription Polymerase Chain Reaction (dpeaa)DE-He213 Target Virus (dpeaa)DE-He213 Immunomagnetic Bead (dpeaa)DE-He213 Capture Capacity (dpeaa)DE-He213 Reverse Transcription Polymerase Chain Reaction Assay (dpeaa)DE-He213 Yin, Shuanghui aut Shang, Youjun aut Wang, Di aut Ma, Weimin aut He, Jijun aut Guo, Jianhong aut Cai, Jianping aut Liu, Xiangtao aut Enthalten in BMC biotechnology London : BioMed Central, 2001 15(2015), 1 vom: 15. Sept. (DE-627)332164837 (DE-600)2052746-9 1472-6750 nnns volume:15 year:2015 number:1 day:15 month:09 https://dx.doi.org/10.1186/s12896-015-0201-5 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2119 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2015 1 15 09
allfields_unstemmed	10.1186/s12896-015-0201-5 doi (DE-627)SPR028440412 (SPR)s12896-015-0201-5-e DE-627 ger DE-627 rakwb eng Yang, Shunli verfasserin aut Specific detection of foot-and-mouth disease serotype Asia 1 virus by carboxyl-magnetic beads conjugated with single-domain antibody 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Yang et al. 2015 Background Immunomagnetic nanobead (IMNB) labeled with specific antibody, has been demonstrated to be useful for the capturing and detection of viruses. Results In this study, we developed an imunomagnetic bead based on carboxyl-magnetic beads (MNB) labeled with a single-domain antibody (sdAb) for capturing foot-and-mouth disease (FMD) Asia 1 virus. After magnetic separation, complexes of MNB-sdAb-virus were detected with either a sandwich ELISA or QDs-C5 probe under a fluorescence microscope, and the complexes were used as templates for extraction of total RNA for amplification of the VP1 or 3D gene fragments using RT-PCR and real-time RT-PCR. The Asia 1 VLPs were efficiently captured through IMNB with a high binding rate of 5.09 μg of antigen/μl of bead suspension. Moreover, this method has been successfully used to capture Asia 1 antigen in synthetic samples. Conclusion Ultimately, a specific and highly sensitive capture FMDV Asia 1 tool has been established that has the potential to enhance the sensitivity and reliability when diagnosing FMDV Asia 1. Reverse Transcription Polymerase Chain Reaction (dpeaa)DE-He213 Target Virus (dpeaa)DE-He213 Immunomagnetic Bead (dpeaa)DE-He213 Capture Capacity (dpeaa)DE-He213 Reverse Transcription Polymerase Chain Reaction Assay (dpeaa)DE-He213 Yin, Shuanghui aut Shang, Youjun aut Wang, Di aut Ma, Weimin aut He, Jijun aut Guo, Jianhong aut Cai, Jianping aut Liu, Xiangtao aut Enthalten in BMC biotechnology London : BioMed Central, 2001 15(2015), 1 vom: 15. Sept. (DE-627)332164837 (DE-600)2052746-9 1472-6750 nnns volume:15 year:2015 number:1 day:15 month:09 https://dx.doi.org/10.1186/s12896-015-0201-5 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2119 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2015 1 15 09
allfieldsGer	10.1186/s12896-015-0201-5 doi (DE-627)SPR028440412 (SPR)s12896-015-0201-5-e DE-627 ger DE-627 rakwb eng Yang, Shunli verfasserin aut Specific detection of foot-and-mouth disease serotype Asia 1 virus by carboxyl-magnetic beads conjugated with single-domain antibody 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Yang et al. 2015 Background Immunomagnetic nanobead (IMNB) labeled with specific antibody, has been demonstrated to be useful for the capturing and detection of viruses. Results In this study, we developed an imunomagnetic bead based on carboxyl-magnetic beads (MNB) labeled with a single-domain antibody (sdAb) for capturing foot-and-mouth disease (FMD) Asia 1 virus. After magnetic separation, complexes of MNB-sdAb-virus were detected with either a sandwich ELISA or QDs-C5 probe under a fluorescence microscope, and the complexes were used as templates for extraction of total RNA for amplification of the VP1 or 3D gene fragments using RT-PCR and real-time RT-PCR. The Asia 1 VLPs were efficiently captured through IMNB with a high binding rate of 5.09 μg of antigen/μl of bead suspension. Moreover, this method has been successfully used to capture Asia 1 antigen in synthetic samples. Conclusion Ultimately, a specific and highly sensitive capture FMDV Asia 1 tool has been established that has the potential to enhance the sensitivity and reliability when diagnosing FMDV Asia 1. Reverse Transcription Polymerase Chain Reaction (dpeaa)DE-He213 Target Virus (dpeaa)DE-He213 Immunomagnetic Bead (dpeaa)DE-He213 Capture Capacity (dpeaa)DE-He213 Reverse Transcription Polymerase Chain Reaction Assay (dpeaa)DE-He213 Yin, Shuanghui aut Shang, Youjun aut Wang, Di aut Ma, Weimin aut He, Jijun aut Guo, Jianhong aut Cai, Jianping aut Liu, Xiangtao aut Enthalten in BMC biotechnology London : BioMed Central, 2001 15(2015), 1 vom: 15. Sept. (DE-627)332164837 (DE-600)2052746-9 1472-6750 nnns volume:15 year:2015 number:1 day:15 month:09 https://dx.doi.org/10.1186/s12896-015-0201-5 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2119 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2015 1 15 09
allfieldsSound	10.1186/s12896-015-0201-5 doi (DE-627)SPR028440412 (SPR)s12896-015-0201-5-e DE-627 ger DE-627 rakwb eng Yang, Shunli verfasserin aut Specific detection of foot-and-mouth disease serotype Asia 1 virus by carboxyl-magnetic beads conjugated with single-domain antibody 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Yang et al. 2015 Background Immunomagnetic nanobead (IMNB) labeled with specific antibody, has been demonstrated to be useful for the capturing and detection of viruses. Results In this study, we developed an imunomagnetic bead based on carboxyl-magnetic beads (MNB) labeled with a single-domain antibody (sdAb) for capturing foot-and-mouth disease (FMD) Asia 1 virus. After magnetic separation, complexes of MNB-sdAb-virus were detected with either a sandwich ELISA or QDs-C5 probe under a fluorescence microscope, and the complexes were used as templates for extraction of total RNA for amplification of the VP1 or 3D gene fragments using RT-PCR and real-time RT-PCR. The Asia 1 VLPs were efficiently captured through IMNB with a high binding rate of 5.09 μg of antigen/μl of bead suspension. Moreover, this method has been successfully used to capture Asia 1 antigen in synthetic samples. Conclusion Ultimately, a specific and highly sensitive capture FMDV Asia 1 tool has been established that has the potential to enhance the sensitivity and reliability when diagnosing FMDV Asia 1. Reverse Transcription Polymerase Chain Reaction (dpeaa)DE-He213 Target Virus (dpeaa)DE-He213 Immunomagnetic Bead (dpeaa)DE-He213 Capture Capacity (dpeaa)DE-He213 Reverse Transcription Polymerase Chain Reaction Assay (dpeaa)DE-He213 Yin, Shuanghui aut Shang, Youjun aut Wang, Di aut Ma, Weimin aut He, Jijun aut Guo, Jianhong aut Cai, Jianping aut Liu, Xiangtao aut Enthalten in BMC biotechnology London : BioMed Central, 2001 15(2015), 1 vom: 15. Sept. (DE-627)332164837 (DE-600)2052746-9 1472-6750 nnns volume:15 year:2015 number:1 day:15 month:09 https://dx.doi.org/10.1186/s12896-015-0201-5 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2119 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2015 1 15 09
language	English
source	Enthalten in BMC biotechnology 15(2015), 1 vom: 15. Sept. volume:15 year:2015 number:1 day:15 month:09
sourceStr	Enthalten in BMC biotechnology 15(2015), 1 vom: 15. Sept. volume:15 year:2015 number:1 day:15 month:09
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Reverse Transcription Polymerase Chain Reaction Target Virus Immunomagnetic Bead Capture Capacity Reverse Transcription Polymerase Chain Reaction Assay
isfreeaccess_bool	true
container_title	BMC biotechnology
authorswithroles_txt_mv	Yang, Shunli @@aut@@ Yin, Shuanghui @@aut@@ Shang, Youjun @@aut@@ Wang, Di @@aut@@ Ma, Weimin @@aut@@ He, Jijun @@aut@@ Guo, Jianhong @@aut@@ Cai, Jianping @@aut@@ Liu, Xiangtao @@aut@@
publishDateDaySort_date	2015-09-15T00:00:00Z
hierarchy_top_id	332164837
id	SPR028440412
language_de	englisch
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author	Yang, Shunli
spellingShingle	Yang, Shunli misc Reverse Transcription Polymerase Chain Reaction misc Target Virus misc Immunomagnetic Bead misc Capture Capacity misc Reverse Transcription Polymerase Chain Reaction Assay Specific detection of foot-and-mouth disease serotype Asia 1 virus by carboxyl-magnetic beads conjugated with single-domain antibody
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topic_title	Specific detection of foot-and-mouth disease serotype Asia 1 virus by carboxyl-magnetic beads conjugated with single-domain antibody Reverse Transcription Polymerase Chain Reaction (dpeaa)DE-He213 Target Virus (dpeaa)DE-He213 Immunomagnetic Bead (dpeaa)DE-He213 Capture Capacity (dpeaa)DE-He213 Reverse Transcription Polymerase Chain Reaction Assay (dpeaa)DE-He213
topic	misc Reverse Transcription Polymerase Chain Reaction misc Target Virus misc Immunomagnetic Bead misc Capture Capacity misc Reverse Transcription Polymerase Chain Reaction Assay
topic_unstemmed	misc Reverse Transcription Polymerase Chain Reaction misc Target Virus misc Immunomagnetic Bead misc Capture Capacity misc Reverse Transcription Polymerase Chain Reaction Assay
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title	Specific detection of foot-and-mouth disease serotype Asia 1 virus by carboxyl-magnetic beads conjugated with single-domain antibody
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title_full	Specific detection of foot-and-mouth disease serotype Asia 1 virus by carboxyl-magnetic beads conjugated with single-domain antibody
author_sort	Yang, Shunli
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author_browse	Yang, Shunli Yin, Shuanghui Shang, Youjun Wang, Di Ma, Weimin He, Jijun Guo, Jianhong Cai, Jianping Liu, Xiangtao
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author-letter	Yang, Shunli
doi_str_mv	10.1186/s12896-015-0201-5
title_sort	specific detection of foot-and-mouth disease serotype asia 1 virus by carboxyl-magnetic beads conjugated with single-domain antibody
title_auth	Specific detection of foot-and-mouth disease serotype Asia 1 virus by carboxyl-magnetic beads conjugated with single-domain antibody
abstract	Background Immunomagnetic nanobead (IMNB) labeled with specific antibody, has been demonstrated to be useful for the capturing and detection of viruses. Results In this study, we developed an imunomagnetic bead based on carboxyl-magnetic beads (MNB) labeled with a single-domain antibody (sdAb) for capturing foot-and-mouth disease (FMD) Asia 1 virus. After magnetic separation, complexes of MNB-sdAb-virus were detected with either a sandwich ELISA or QDs-C5 probe under a fluorescence microscope, and the complexes were used as templates for extraction of total RNA for amplification of the VP1 or 3D gene fragments using RT-PCR and real-time RT-PCR. The Asia 1 VLPs were efficiently captured through IMNB with a high binding rate of 5.09 μg of antigen/μl of bead suspension. Moreover, this method has been successfully used to capture Asia 1 antigen in synthetic samples. Conclusion Ultimately, a specific and highly sensitive capture FMDV Asia 1 tool has been established that has the potential to enhance the sensitivity and reliability when diagnosing FMDV Asia 1. © Yang et al. 2015
abstractGer	Background Immunomagnetic nanobead (IMNB) labeled with specific antibody, has been demonstrated to be useful for the capturing and detection of viruses. Results In this study, we developed an imunomagnetic bead based on carboxyl-magnetic beads (MNB) labeled with a single-domain antibody (sdAb) for capturing foot-and-mouth disease (FMD) Asia 1 virus. After magnetic separation, complexes of MNB-sdAb-virus were detected with either a sandwich ELISA or QDs-C5 probe under a fluorescence microscope, and the complexes were used as templates for extraction of total RNA for amplification of the VP1 or 3D gene fragments using RT-PCR and real-time RT-PCR. The Asia 1 VLPs were efficiently captured through IMNB with a high binding rate of 5.09 μg of antigen/μl of bead suspension. Moreover, this method has been successfully used to capture Asia 1 antigen in synthetic samples. Conclusion Ultimately, a specific and highly sensitive capture FMDV Asia 1 tool has been established that has the potential to enhance the sensitivity and reliability when diagnosing FMDV Asia 1. © Yang et al. 2015
abstract_unstemmed	Background Immunomagnetic nanobead (IMNB) labeled with specific antibody, has been demonstrated to be useful for the capturing and detection of viruses. Results In this study, we developed an imunomagnetic bead based on carboxyl-magnetic beads (MNB) labeled with a single-domain antibody (sdAb) for capturing foot-and-mouth disease (FMD) Asia 1 virus. After magnetic separation, complexes of MNB-sdAb-virus were detected with either a sandwich ELISA or QDs-C5 probe under a fluorescence microscope, and the complexes were used as templates for extraction of total RNA for amplification of the VP1 or 3D gene fragments using RT-PCR and real-time RT-PCR. The Asia 1 VLPs were efficiently captured through IMNB with a high binding rate of 5.09 μg of antigen/μl of bead suspension. Moreover, this method has been successfully used to capture Asia 1 antigen in synthetic samples. Conclusion Ultimately, a specific and highly sensitive capture FMDV Asia 1 tool has been established that has the potential to enhance the sensitivity and reliability when diagnosing FMDV Asia 1. © Yang et al. 2015
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title_short	Specific detection of foot-and-mouth disease serotype Asia 1 virus by carboxyl-magnetic beads conjugated with single-domain antibody
url	https://dx.doi.org/10.1186/s12896-015-0201-5
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author2	Yin, Shuanghui Shang, Youjun Wang, Di Ma, Weimin He, Jijun Guo, Jianhong Cai, Jianping Liu, Xiangtao
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up_date	2024-07-03T19:29:01.963Z
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Specific detection of foot-and-mouth disease serotype Asia 1 virus by carboxyl-magnetic beads conjugated with single-domain antibody

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