Identification and characterization of the GmRD26 soybean promoter in response to abiotic stresses: potential tool for biotechnological application

GUS (2.054 bp) and two promoter modules, pGmRD26A::GUS (909 pb) and pGmRD26B::GUS (435 bp), controlling the expression of the β-glucuronidase (uidA) gene. Analysis of GUS activity has demonstrated that pGmRD26 and pGmRD26A induce strong reporter gene expression, as the pAtRD29 positive control promo...
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Autor*in:	Freitas, Elinea O. [verfasserIn] Melo, Bruno P. Lourenço-Tessutti, Isabela T. Arraes, Fabrício B. M. Amorim, Regina M. Lisei-de-Sá, Maria E. Costa, Julia A. Leite, Ana G. B. Faheem, Muhammad Ferreira, Márcio A. Morgante, Carolina V. Fontes, Elizabeth P. B. Grossi-de-Sa, Maria F.

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2019

Schlagwörter:	Stress-responsive promoter Drought tolerance Abscisic acid Promoter modules analysis Gene-promoter characterization

Anmerkung:	© The Author(s). 2019

Übergeordnetes Werk:	Enthalten in: BMC biotechnology - London : BioMed Central, 2001, 19(2019), 1 vom: 20. Nov.
Übergeordnetes Werk:	volume:19 ; year:2019 ; number:1 ; day:20 ; month:11

Links:	Volltext

DOI / URN:	10.1186/s12896-019-0561-3

Katalog-ID:	SPR028480457

Internformat


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041			\|a eng
100	1		\|a Freitas, Elinea O. \|e verfasserin \|4 aut
245	1	0	\|a Identification and characterization of the GmRD26 soybean promoter in response to abiotic stresses: potential tool for biotechnological application
264		1	\|c 2019
336			\|a Text \|b txt \|2 rdacontent
337			\|a Computermedien \|b c \|2 rdamedia
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520			\|a Background Drought is one of the most harmful abiotic stresses for plants, leading to reduced productivity of several economically important crops and, consequently, considerable losses in the agricultural sector. When plants are exposed to stressful conditions, such as drought and high salinity, they modulate the expression of genes that lead to developmental, biochemical, and physiological changes, which help to overcome the deleterious effects of adverse circumstances. Thus, the search for new specific gene promoter sequences has proved to be a powerful biotechnological strategy to control the expression of key genes involved in water deprivation or multiple stress responses. Results This study aimed to identify and characterize the GmRD26 promoter (pGmRD26), which is involved in the regulation of plant responses to drought stress. The expression profile of the GmRD26 gene was investigated by qRT-PCR under normal and stress conditions in Williams 82, BR16 and Embrapa48 soybean-cultivars. Our data confirm that GmRD26 is induced under water deficit with different induction folds between analyzed cultivars, which display different genetic background and physiological behaviour under drought. The characterization of the GmRD26 promoter was performed under simulated stress conditions with abscisic acid (ABA), polyethylene glycol (PEG) and drought (air dry) on A. thaliana plants containing the complete construct of pGmRD26::GUS (2.054 bp) and two promoter modules, pGmRD26A::GUS (909 pb) and pGmRD26B::GUS (435 bp), controlling the expression of the β-glucuronidase (uidA) gene. Analysis of GUS activity has demonstrated that pGmRD26 and pGmRD26A induce strong reporter gene expression, as the pAtRD29 positive control promoter under ABA and PEG treatment. Conclusions The full-length promoter pGmRD26 and the pGmRD26A module provides an improved uidA transcription capacity when compared with the other promoter module, especially in response to polyethylene glycol and drought treatments. These data indicate that pGmRD26A may become a promising biotechnological asset with potential use in the development of modified drought-tolerant plants or other plants designed for stress responses.
650		4	\|a Stress-responsive promoter \|7 (dpeaa)DE-He213
650		4	\|a Drought tolerance \|7 (dpeaa)DE-He213
650		4	\|a Abscisic acid \|7 (dpeaa)DE-He213
650		4	\|a Promoter modules analysis \|7 (dpeaa)DE-He213
650		4	\|a Gene-promoter characterization \|7 (dpeaa)DE-He213
700	1		\|a Melo, Bruno P. \|4 aut
700	1		\|a Lourenço-Tessutti, Isabela T. \|4 aut
700	1		\|a Arraes, Fabrício B. M. \|4 aut
700	1		\|a Amorim, Regina M. \|4 aut
700	1		\|a Lisei-de-Sá, Maria E. \|0 (orcid)0000-0002-2710-1368 \|4 aut
700	1		\|a Costa, Julia A. \|4 aut
700	1		\|a Leite, Ana G. B. \|4 aut
700	1		\|a Faheem, Muhammad \|4 aut
700	1		\|a Ferreira, Márcio A. \|4 aut
700	1		\|a Morgante, Carolina V. \|4 aut
700	1		\|a Fontes, Elizabeth P. B. \|4 aut
700	1		\|a Grossi-de-Sa, Maria F. \|4 aut
773	0	8	\|i Enthalten in \|t BMC biotechnology \|d London : BioMed Central, 2001 \|g 19(2019), 1 vom: 20. Nov. \|w (DE-627)332164837 \|w (DE-600)2052746-9 \|x 1472-6750 \|7 nnns
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912			\|a GBV_ILN_24
912			\|a GBV_ILN_39
912			\|a GBV_ILN_40
912			\|a GBV_ILN_60
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912			\|a GBV_ILN_63
912			\|a GBV_ILN_65
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912			\|a GBV_ILN_224
912			\|a GBV_ILN_230
912			\|a GBV_ILN_285
912			\|a GBV_ILN_293
912			\|a GBV_ILN_602
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912			\|a GBV_ILN_2056
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912			\|a GBV_ILN_2111
912			\|a GBV_ILN_2113
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912			\|a GBV_ILN_2190
912			\|a GBV_ILN_4012
912			\|a GBV_ILN_4037
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912			\|a GBV_ILN_4249
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912			\|a GBV_ILN_4306
912			\|a GBV_ILN_4307
912			\|a GBV_ILN_4313
912			\|a GBV_ILN_4322
912			\|a GBV_ILN_4323
912			\|a GBV_ILN_4324
912			\|a GBV_ILN_4325
912			\|a GBV_ILN_4338
912			\|a GBV_ILN_4367
912			\|a GBV_ILN_4700
951			\|a AR
952			\|d 19 \|j 2019 \|e 1 \|b 20 \|c 11

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allfields	10.1186/s12896-019-0561-3 doi (DE-627)SPR028480457 (SPR)s12896-019-0561-3-e DE-627 ger DE-627 rakwb eng Freitas, Elinea O. verfasserin aut Identification and characterization of the GmRD26 soybean promoter in response to abiotic stresses: potential tool for biotechnological application 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2019 Background Drought is one of the most harmful abiotic stresses for plants, leading to reduced productivity of several economically important crops and, consequently, considerable losses in the agricultural sector. When plants are exposed to stressful conditions, such as drought and high salinity, they modulate the expression of genes that lead to developmental, biochemical, and physiological changes, which help to overcome the deleterious effects of adverse circumstances. Thus, the search for new specific gene promoter sequences has proved to be a powerful biotechnological strategy to control the expression of key genes involved in water deprivation or multiple stress responses. Results This study aimed to identify and characterize the GmRD26 promoter (pGmRD26), which is involved in the regulation of plant responses to drought stress. The expression profile of the GmRD26 gene was investigated by qRT-PCR under normal and stress conditions in Williams 82, BR16 and Embrapa48 soybean-cultivars. Our data confirm that GmRD26 is induced under water deficit with different induction folds between analyzed cultivars, which display different genetic background and physiological behaviour under drought. The characterization of the GmRD26 promoter was performed under simulated stress conditions with abscisic acid (ABA), polyethylene glycol (PEG) and drought (air dry) on A. thaliana plants containing the complete construct of pGmRD26::GUS (2.054 bp) and two promoter modules, pGmRD26A::GUS (909 pb) and pGmRD26B::GUS (435 bp), controlling the expression of the β-glucuronidase (uidA) gene. Analysis of GUS activity has demonstrated that pGmRD26 and pGmRD26A induce strong reporter gene expression, as the pAtRD29 positive control promoter under ABA and PEG treatment. Conclusions The full-length promoter pGmRD26 and the pGmRD26A module provides an improved uidA transcription capacity when compared with the other promoter module, especially in response to polyethylene glycol and drought treatments. These data indicate that pGmRD26A may become a promising biotechnological asset with potential use in the development of modified drought-tolerant plants or other plants designed for stress responses. Stress-responsive promoter (dpeaa)DE-He213 Drought tolerance (dpeaa)DE-He213 Abscisic acid (dpeaa)DE-He213 Promoter modules analysis (dpeaa)DE-He213 Gene-promoter characterization (dpeaa)DE-He213 Melo, Bruno P. aut Lourenço-Tessutti, Isabela T. aut Arraes, Fabrício B. M. aut Amorim, Regina M. aut Lisei-de-Sá, Maria E. (orcid)0000-0002-2710-1368 aut Costa, Julia A. aut Leite, Ana G. B. aut Faheem, Muhammad aut Ferreira, Márcio A. aut Morgante, Carolina V. aut Fontes, Elizabeth P. B. aut Grossi-de-Sa, Maria F. aut Enthalten in BMC biotechnology London : BioMed Central, 2001 19(2019), 1 vom: 20. Nov. (DE-627)332164837 (DE-600)2052746-9 1472-6750 nnns volume:19 year:2019 number:1 day:20 month:11 https://dx.doi.org/10.1186/s12896-019-0561-3 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2119 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 19 2019 1 20 11
spelling	10.1186/s12896-019-0561-3 doi (DE-627)SPR028480457 (SPR)s12896-019-0561-3-e DE-627 ger DE-627 rakwb eng Freitas, Elinea O. verfasserin aut Identification and characterization of the GmRD26 soybean promoter in response to abiotic stresses: potential tool for biotechnological application 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2019 Background Drought is one of the most harmful abiotic stresses for plants, leading to reduced productivity of several economically important crops and, consequently, considerable losses in the agricultural sector. When plants are exposed to stressful conditions, such as drought and high salinity, they modulate the expression of genes that lead to developmental, biochemical, and physiological changes, which help to overcome the deleterious effects of adverse circumstances. Thus, the search for new specific gene promoter sequences has proved to be a powerful biotechnological strategy to control the expression of key genes involved in water deprivation or multiple stress responses. Results This study aimed to identify and characterize the GmRD26 promoter (pGmRD26), which is involved in the regulation of plant responses to drought stress. The expression profile of the GmRD26 gene was investigated by qRT-PCR under normal and stress conditions in Williams 82, BR16 and Embrapa48 soybean-cultivars. Our data confirm that GmRD26 is induced under water deficit with different induction folds between analyzed cultivars, which display different genetic background and physiological behaviour under drought. The characterization of the GmRD26 promoter was performed under simulated stress conditions with abscisic acid (ABA), polyethylene glycol (PEG) and drought (air dry) on A. thaliana plants containing the complete construct of pGmRD26::GUS (2.054 bp) and two promoter modules, pGmRD26A::GUS (909 pb) and pGmRD26B::GUS (435 bp), controlling the expression of the β-glucuronidase (uidA) gene. Analysis of GUS activity has demonstrated that pGmRD26 and pGmRD26A induce strong reporter gene expression, as the pAtRD29 positive control promoter under ABA and PEG treatment. Conclusions The full-length promoter pGmRD26 and the pGmRD26A module provides an improved uidA transcription capacity when compared with the other promoter module, especially in response to polyethylene glycol and drought treatments. These data indicate that pGmRD26A may become a promising biotechnological asset with potential use in the development of modified drought-tolerant plants or other plants designed for stress responses. Stress-responsive promoter (dpeaa)DE-He213 Drought tolerance (dpeaa)DE-He213 Abscisic acid (dpeaa)DE-He213 Promoter modules analysis (dpeaa)DE-He213 Gene-promoter characterization (dpeaa)DE-He213 Melo, Bruno P. aut Lourenço-Tessutti, Isabela T. aut Arraes, Fabrício B. M. aut Amorim, Regina M. aut Lisei-de-Sá, Maria E. (orcid)0000-0002-2710-1368 aut Costa, Julia A. aut Leite, Ana G. B. aut Faheem, Muhammad aut Ferreira, Márcio A. aut Morgante, Carolina V. aut Fontes, Elizabeth P. B. aut Grossi-de-Sa, Maria F. aut Enthalten in BMC biotechnology London : BioMed Central, 2001 19(2019), 1 vom: 20. Nov. (DE-627)332164837 (DE-600)2052746-9 1472-6750 nnns volume:19 year:2019 number:1 day:20 month:11 https://dx.doi.org/10.1186/s12896-019-0561-3 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2119 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 19 2019 1 20 11
allfields_unstemmed	10.1186/s12896-019-0561-3 doi (DE-627)SPR028480457 (SPR)s12896-019-0561-3-e DE-627 ger DE-627 rakwb eng Freitas, Elinea O. verfasserin aut Identification and characterization of the GmRD26 soybean promoter in response to abiotic stresses: potential tool for biotechnological application 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2019 Background Drought is one of the most harmful abiotic stresses for plants, leading to reduced productivity of several economically important crops and, consequently, considerable losses in the agricultural sector. When plants are exposed to stressful conditions, such as drought and high salinity, they modulate the expression of genes that lead to developmental, biochemical, and physiological changes, which help to overcome the deleterious effects of adverse circumstances. Thus, the search for new specific gene promoter sequences has proved to be a powerful biotechnological strategy to control the expression of key genes involved in water deprivation or multiple stress responses. Results This study aimed to identify and characterize the GmRD26 promoter (pGmRD26), which is involved in the regulation of plant responses to drought stress. The expression profile of the GmRD26 gene was investigated by qRT-PCR under normal and stress conditions in Williams 82, BR16 and Embrapa48 soybean-cultivars. Our data confirm that GmRD26 is induced under water deficit with different induction folds between analyzed cultivars, which display different genetic background and physiological behaviour under drought. The characterization of the GmRD26 promoter was performed under simulated stress conditions with abscisic acid (ABA), polyethylene glycol (PEG) and drought (air dry) on A. thaliana plants containing the complete construct of pGmRD26::GUS (2.054 bp) and two promoter modules, pGmRD26A::GUS (909 pb) and pGmRD26B::GUS (435 bp), controlling the expression of the β-glucuronidase (uidA) gene. Analysis of GUS activity has demonstrated that pGmRD26 and pGmRD26A induce strong reporter gene expression, as the pAtRD29 positive control promoter under ABA and PEG treatment. Conclusions The full-length promoter pGmRD26 and the pGmRD26A module provides an improved uidA transcription capacity when compared with the other promoter module, especially in response to polyethylene glycol and drought treatments. These data indicate that pGmRD26A may become a promising biotechnological asset with potential use in the development of modified drought-tolerant plants or other plants designed for stress responses. Stress-responsive promoter (dpeaa)DE-He213 Drought tolerance (dpeaa)DE-He213 Abscisic acid (dpeaa)DE-He213 Promoter modules analysis (dpeaa)DE-He213 Gene-promoter characterization (dpeaa)DE-He213 Melo, Bruno P. aut Lourenço-Tessutti, Isabela T. aut Arraes, Fabrício B. M. aut Amorim, Regina M. aut Lisei-de-Sá, Maria E. (orcid)0000-0002-2710-1368 aut Costa, Julia A. aut Leite, Ana G. B. aut Faheem, Muhammad aut Ferreira, Márcio A. aut Morgante, Carolina V. aut Fontes, Elizabeth P. B. aut Grossi-de-Sa, Maria F. aut Enthalten in BMC biotechnology London : BioMed Central, 2001 19(2019), 1 vom: 20. Nov. (DE-627)332164837 (DE-600)2052746-9 1472-6750 nnns volume:19 year:2019 number:1 day:20 month:11 https://dx.doi.org/10.1186/s12896-019-0561-3 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2119 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 19 2019 1 20 11
allfieldsGer	10.1186/s12896-019-0561-3 doi (DE-627)SPR028480457 (SPR)s12896-019-0561-3-e DE-627 ger DE-627 rakwb eng Freitas, Elinea O. verfasserin aut Identification and characterization of the GmRD26 soybean promoter in response to abiotic stresses: potential tool for biotechnological application 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2019 Background Drought is one of the most harmful abiotic stresses for plants, leading to reduced productivity of several economically important crops and, consequently, considerable losses in the agricultural sector. When plants are exposed to stressful conditions, such as drought and high salinity, they modulate the expression of genes that lead to developmental, biochemical, and physiological changes, which help to overcome the deleterious effects of adverse circumstances. Thus, the search for new specific gene promoter sequences has proved to be a powerful biotechnological strategy to control the expression of key genes involved in water deprivation or multiple stress responses. Results This study aimed to identify and characterize the GmRD26 promoter (pGmRD26), which is involved in the regulation of plant responses to drought stress. The expression profile of the GmRD26 gene was investigated by qRT-PCR under normal and stress conditions in Williams 82, BR16 and Embrapa48 soybean-cultivars. Our data confirm that GmRD26 is induced under water deficit with different induction folds between analyzed cultivars, which display different genetic background and physiological behaviour under drought. The characterization of the GmRD26 promoter was performed under simulated stress conditions with abscisic acid (ABA), polyethylene glycol (PEG) and drought (air dry) on A. thaliana plants containing the complete construct of pGmRD26::GUS (2.054 bp) and two promoter modules, pGmRD26A::GUS (909 pb) and pGmRD26B::GUS (435 bp), controlling the expression of the β-glucuronidase (uidA) gene. Analysis of GUS activity has demonstrated that pGmRD26 and pGmRD26A induce strong reporter gene expression, as the pAtRD29 positive control promoter under ABA and PEG treatment. Conclusions The full-length promoter pGmRD26 and the pGmRD26A module provides an improved uidA transcription capacity when compared with the other promoter module, especially in response to polyethylene glycol and drought treatments. These data indicate that pGmRD26A may become a promising biotechnological asset with potential use in the development of modified drought-tolerant plants or other plants designed for stress responses. Stress-responsive promoter (dpeaa)DE-He213 Drought tolerance (dpeaa)DE-He213 Abscisic acid (dpeaa)DE-He213 Promoter modules analysis (dpeaa)DE-He213 Gene-promoter characterization (dpeaa)DE-He213 Melo, Bruno P. aut Lourenço-Tessutti, Isabela T. aut Arraes, Fabrício B. M. aut Amorim, Regina M. aut Lisei-de-Sá, Maria E. (orcid)0000-0002-2710-1368 aut Costa, Julia A. aut Leite, Ana G. B. aut Faheem, Muhammad aut Ferreira, Márcio A. aut Morgante, Carolina V. aut Fontes, Elizabeth P. B. aut Grossi-de-Sa, Maria F. aut Enthalten in BMC biotechnology London : BioMed Central, 2001 19(2019), 1 vom: 20. Nov. (DE-627)332164837 (DE-600)2052746-9 1472-6750 nnns volume:19 year:2019 number:1 day:20 month:11 https://dx.doi.org/10.1186/s12896-019-0561-3 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2119 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 19 2019 1 20 11
allfieldsSound	10.1186/s12896-019-0561-3 doi (DE-627)SPR028480457 (SPR)s12896-019-0561-3-e DE-627 ger DE-627 rakwb eng Freitas, Elinea O. verfasserin aut Identification and characterization of the GmRD26 soybean promoter in response to abiotic stresses: potential tool for biotechnological application 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2019 Background Drought is one of the most harmful abiotic stresses for plants, leading to reduced productivity of several economically important crops and, consequently, considerable losses in the agricultural sector. When plants are exposed to stressful conditions, such as drought and high salinity, they modulate the expression of genes that lead to developmental, biochemical, and physiological changes, which help to overcome the deleterious effects of adverse circumstances. Thus, the search for new specific gene promoter sequences has proved to be a powerful biotechnological strategy to control the expression of key genes involved in water deprivation or multiple stress responses. Results This study aimed to identify and characterize the GmRD26 promoter (pGmRD26), which is involved in the regulation of plant responses to drought stress. The expression profile of the GmRD26 gene was investigated by qRT-PCR under normal and stress conditions in Williams 82, BR16 and Embrapa48 soybean-cultivars. Our data confirm that GmRD26 is induced under water deficit with different induction folds between analyzed cultivars, which display different genetic background and physiological behaviour under drought. The characterization of the GmRD26 promoter was performed under simulated stress conditions with abscisic acid (ABA), polyethylene glycol (PEG) and drought (air dry) on A. thaliana plants containing the complete construct of pGmRD26::GUS (2.054 bp) and two promoter modules, pGmRD26A::GUS (909 pb) and pGmRD26B::GUS (435 bp), controlling the expression of the β-glucuronidase (uidA) gene. Analysis of GUS activity has demonstrated that pGmRD26 and pGmRD26A induce strong reporter gene expression, as the pAtRD29 positive control promoter under ABA and PEG treatment. Conclusions The full-length promoter pGmRD26 and the pGmRD26A module provides an improved uidA transcription capacity when compared with the other promoter module, especially in response to polyethylene glycol and drought treatments. These data indicate that pGmRD26A may become a promising biotechnological asset with potential use in the development of modified drought-tolerant plants or other plants designed for stress responses. Stress-responsive promoter (dpeaa)DE-He213 Drought tolerance (dpeaa)DE-He213 Abscisic acid (dpeaa)DE-He213 Promoter modules analysis (dpeaa)DE-He213 Gene-promoter characterization (dpeaa)DE-He213 Melo, Bruno P. aut Lourenço-Tessutti, Isabela T. aut Arraes, Fabrício B. M. aut Amorim, Regina M. aut Lisei-de-Sá, Maria E. (orcid)0000-0002-2710-1368 aut Costa, Julia A. aut Leite, Ana G. B. aut Faheem, Muhammad aut Ferreira, Márcio A. aut Morgante, Carolina V. aut Fontes, Elizabeth P. B. aut Grossi-de-Sa, Maria F. aut Enthalten in BMC biotechnology London : BioMed Central, 2001 19(2019), 1 vom: 20. Nov. (DE-627)332164837 (DE-600)2052746-9 1472-6750 nnns volume:19 year:2019 number:1 day:20 month:11 https://dx.doi.org/10.1186/s12896-019-0561-3 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2119 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 19 2019 1 20 11
language	English
source	Enthalten in BMC biotechnology 19(2019), 1 vom: 20. Nov. volume:19 year:2019 number:1 day:20 month:11
sourceStr	Enthalten in BMC biotechnology 19(2019), 1 vom: 20. Nov. volume:19 year:2019 number:1 day:20 month:11
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Stress-responsive promoter Drought tolerance Abscisic acid Promoter modules analysis Gene-promoter characterization
isfreeaccess_bool	true
container_title	BMC biotechnology
authorswithroles_txt_mv	Freitas, Elinea O. @@aut@@ Melo, Bruno P. @@aut@@ Lourenço-Tessutti, Isabela T. @@aut@@ Arraes, Fabrício B. M. @@aut@@ Amorim, Regina M. @@aut@@ Lisei-de-Sá, Maria E. @@aut@@ Costa, Julia A. @@aut@@ Leite, Ana G. B. @@aut@@ Faheem, Muhammad @@aut@@ Ferreira, Márcio A. @@aut@@ Morgante, Carolina V. @@aut@@ Fontes, Elizabeth P. B. @@aut@@ Grossi-de-Sa, Maria F. @@aut@@
publishDateDaySort_date	2019-11-20T00:00:00Z
hierarchy_top_id	332164837
id	SPR028480457
language_de	englisch
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When plants are exposed to stressful conditions, such as drought and high salinity, they modulate the expression of genes that lead to developmental, biochemical, and physiological changes, which help to overcome the deleterious effects of adverse circumstances. Thus, the search for new specific gene promoter sequences has proved to be a powerful biotechnological strategy to control the expression of key genes involved in water deprivation or multiple stress responses. Results This study aimed to identify and characterize the GmRD26 promoter (pGmRD26), which is involved in the regulation of plant responses to drought stress. The expression profile of the GmRD26 gene was investigated by qRT-PCR under normal and stress conditions in Williams 82, BR16 and Embrapa48 soybean-cultivars. Our data confirm that GmRD26 is induced under water deficit with different induction folds between analyzed cultivars, which display different genetic background and physiological behaviour under drought. The characterization of the GmRD26 promoter was performed under simulated stress conditions with abscisic acid (ABA), polyethylene glycol (PEG) and drought (air dry) on A. thaliana plants containing the complete construct of pGmRD26::GUS (2.054 bp) and two promoter modules, pGmRD26A::GUS (909 pb) and pGmRD26B::GUS (435 bp), controlling the expression of the β-glucuronidase (uidA) gene. Analysis of GUS activity has demonstrated that pGmRD26 and pGmRD26A induce strong reporter gene expression, as the pAtRD29 positive control promoter under ABA and PEG treatment. Conclusions The full-length promoter pGmRD26 and the pGmRD26A module provides an improved uidA transcription capacity when compared with the other promoter module, especially in response to polyethylene glycol and drought treatments. 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author	Freitas, Elinea O.
spellingShingle	Freitas, Elinea O. misc Stress-responsive promoter misc Drought tolerance misc Abscisic acid misc Promoter modules analysis misc Gene-promoter characterization Identification and characterization of the GmRD26 soybean promoter in response to abiotic stresses: potential tool for biotechnological application
authorStr	Freitas, Elinea O.
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topic_title	Identification and characterization of the GmRD26 soybean promoter in response to abiotic stresses: potential tool for biotechnological application Stress-responsive promoter (dpeaa)DE-He213 Drought tolerance (dpeaa)DE-He213 Abscisic acid (dpeaa)DE-He213 Promoter modules analysis (dpeaa)DE-He213 Gene-promoter characterization (dpeaa)DE-He213
topic	misc Stress-responsive promoter misc Drought tolerance misc Abscisic acid misc Promoter modules analysis misc Gene-promoter characterization
topic_unstemmed	misc Stress-responsive promoter misc Drought tolerance misc Abscisic acid misc Promoter modules analysis misc Gene-promoter characterization
topic_browse	misc Stress-responsive promoter misc Drought tolerance misc Abscisic acid misc Promoter modules analysis misc Gene-promoter characterization
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title	Identification and characterization of the GmRD26 soybean promoter in response to abiotic stresses: potential tool for biotechnological application
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title_full	Identification and characterization of the GmRD26 soybean promoter in response to abiotic stresses: potential tool for biotechnological application
author_sort	Freitas, Elinea O.
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author_browse	Freitas, Elinea O. Melo, Bruno P. Lourenço-Tessutti, Isabela T. Arraes, Fabrício B. M. Amorim, Regina M. Lisei-de-Sá, Maria E. Costa, Julia A. Leite, Ana G. B. Faheem, Muhammad Ferreira, Márcio A. Morgante, Carolina V. Fontes, Elizabeth P. B. Grossi-de-Sa, Maria F.
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author-letter	Freitas, Elinea O.
doi_str_mv	10.1186/s12896-019-0561-3
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title_sort	identification and characterization of the gmrd26 soybean promoter in response to abiotic stresses: potential tool for biotechnological application
title_auth	Identification and characterization of the GmRD26 soybean promoter in response to abiotic stresses: potential tool for biotechnological application
abstract	Background Drought is one of the most harmful abiotic stresses for plants, leading to reduced productivity of several economically important crops and, consequently, considerable losses in the agricultural sector. When plants are exposed to stressful conditions, such as drought and high salinity, they modulate the expression of genes that lead to developmental, biochemical, and physiological changes, which help to overcome the deleterious effects of adverse circumstances. Thus, the search for new specific gene promoter sequences has proved to be a powerful biotechnological strategy to control the expression of key genes involved in water deprivation or multiple stress responses. Results This study aimed to identify and characterize the GmRD26 promoter (pGmRD26), which is involved in the regulation of plant responses to drought stress. The expression profile of the GmRD26 gene was investigated by qRT-PCR under normal and stress conditions in Williams 82, BR16 and Embrapa48 soybean-cultivars. Our data confirm that GmRD26 is induced under water deficit with different induction folds between analyzed cultivars, which display different genetic background and physiological behaviour under drought. The characterization of the GmRD26 promoter was performed under simulated stress conditions with abscisic acid (ABA), polyethylene glycol (PEG) and drought (air dry) on A. thaliana plants containing the complete construct of pGmRD26::GUS (2.054 bp) and two promoter modules, pGmRD26A::GUS (909 pb) and pGmRD26B::GUS (435 bp), controlling the expression of the β-glucuronidase (uidA) gene. Analysis of GUS activity has demonstrated that pGmRD26 and pGmRD26A induce strong reporter gene expression, as the pAtRD29 positive control promoter under ABA and PEG treatment. Conclusions The full-length promoter pGmRD26 and the pGmRD26A module provides an improved uidA transcription capacity when compared with the other promoter module, especially in response to polyethylene glycol and drought treatments. These data indicate that pGmRD26A may become a promising biotechnological asset with potential use in the development of modified drought-tolerant plants or other plants designed for stress responses. © The Author(s). 2019
abstractGer	Background Drought is one of the most harmful abiotic stresses for plants, leading to reduced productivity of several economically important crops and, consequently, considerable losses in the agricultural sector. When plants are exposed to stressful conditions, such as drought and high salinity, they modulate the expression of genes that lead to developmental, biochemical, and physiological changes, which help to overcome the deleterious effects of adverse circumstances. Thus, the search for new specific gene promoter sequences has proved to be a powerful biotechnological strategy to control the expression of key genes involved in water deprivation or multiple stress responses. Results This study aimed to identify and characterize the GmRD26 promoter (pGmRD26), which is involved in the regulation of plant responses to drought stress. The expression profile of the GmRD26 gene was investigated by qRT-PCR under normal and stress conditions in Williams 82, BR16 and Embrapa48 soybean-cultivars. Our data confirm that GmRD26 is induced under water deficit with different induction folds between analyzed cultivars, which display different genetic background and physiological behaviour under drought. The characterization of the GmRD26 promoter was performed under simulated stress conditions with abscisic acid (ABA), polyethylene glycol (PEG) and drought (air dry) on A. thaliana plants containing the complete construct of pGmRD26::GUS (2.054 bp) and two promoter modules, pGmRD26A::GUS (909 pb) and pGmRD26B::GUS (435 bp), controlling the expression of the β-glucuronidase (uidA) gene. Analysis of GUS activity has demonstrated that pGmRD26 and pGmRD26A induce strong reporter gene expression, as the pAtRD29 positive control promoter under ABA and PEG treatment. Conclusions The full-length promoter pGmRD26 and the pGmRD26A module provides an improved uidA transcription capacity when compared with the other promoter module, especially in response to polyethylene glycol and drought treatments. These data indicate that pGmRD26A may become a promising biotechnological asset with potential use in the development of modified drought-tolerant plants or other plants designed for stress responses. © The Author(s). 2019
abstract_unstemmed	Background Drought is one of the most harmful abiotic stresses for plants, leading to reduced productivity of several economically important crops and, consequently, considerable losses in the agricultural sector. When plants are exposed to stressful conditions, such as drought and high salinity, they modulate the expression of genes that lead to developmental, biochemical, and physiological changes, which help to overcome the deleterious effects of adverse circumstances. Thus, the search for new specific gene promoter sequences has proved to be a powerful biotechnological strategy to control the expression of key genes involved in water deprivation or multiple stress responses. Results This study aimed to identify and characterize the GmRD26 promoter (pGmRD26), which is involved in the regulation of plant responses to drought stress. The expression profile of the GmRD26 gene was investigated by qRT-PCR under normal and stress conditions in Williams 82, BR16 and Embrapa48 soybean-cultivars. Our data confirm that GmRD26 is induced under water deficit with different induction folds between analyzed cultivars, which display different genetic background and physiological behaviour under drought. The characterization of the GmRD26 promoter was performed under simulated stress conditions with abscisic acid (ABA), polyethylene glycol (PEG) and drought (air dry) on A. thaliana plants containing the complete construct of pGmRD26::GUS (2.054 bp) and two promoter modules, pGmRD26A::GUS (909 pb) and pGmRD26B::GUS (435 bp), controlling the expression of the β-glucuronidase (uidA) gene. Analysis of GUS activity has demonstrated that pGmRD26 and pGmRD26A induce strong reporter gene expression, as the pAtRD29 positive control promoter under ABA and PEG treatment. Conclusions The full-length promoter pGmRD26 and the pGmRD26A module provides an improved uidA transcription capacity when compared with the other promoter module, especially in response to polyethylene glycol and drought treatments. These data indicate that pGmRD26A may become a promising biotechnological asset with potential use in the development of modified drought-tolerant plants or other plants designed for stress responses. © The Author(s). 2019
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title_short	Identification and characterization of the GmRD26 soybean promoter in response to abiotic stresses: potential tool for biotechnological application
url	https://dx.doi.org/10.1186/s12896-019-0561-3
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author2	Melo, Bruno P. Lourenço-Tessutti, Isabela T. Arraes, Fabrício B. M. Amorim, Regina M. Lisei-de-Sá, Maria E. Costa, Julia A. Leite, Ana G. B. Faheem, Muhammad Ferreira, Márcio A. Morgante, Carolina V. Fontes, Elizabeth P. B. Grossi-de-Sa, Maria F.
author2Str	Melo, Bruno P. Lourenço-Tessutti, Isabela T. Arraes, Fabrício B. M. Amorim, Regina M. Lisei-de-Sá, Maria E. Costa, Julia A. Leite, Ana G. B. Faheem, Muhammad Ferreira, Márcio A. Morgante, Carolina V. Fontes, Elizabeth P. B. Grossi-de-Sa, Maria F.
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up_date	2024-07-03T19:41:43.398Z
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When plants are exposed to stressful conditions, such as drought and high salinity, they modulate the expression of genes that lead to developmental, biochemical, and physiological changes, which help to overcome the deleterious effects of adverse circumstances. Thus, the search for new specific gene promoter sequences has proved to be a powerful biotechnological strategy to control the expression of key genes involved in water deprivation or multiple stress responses. Results This study aimed to identify and characterize the GmRD26 promoter (pGmRD26), which is involved in the regulation of plant responses to drought stress. The expression profile of the GmRD26 gene was investigated by qRT-PCR under normal and stress conditions in Williams 82, BR16 and Embrapa48 soybean-cultivars. Our data confirm that GmRD26 is induced under water deficit with different induction folds between analyzed cultivars, which display different genetic background and physiological behaviour under drought. The characterization of the GmRD26 promoter was performed under simulated stress conditions with abscisic acid (ABA), polyethylene glycol (PEG) and drought (air dry) on A. thaliana plants containing the complete construct of pGmRD26::GUS (2.054 bp) and two promoter modules, pGmRD26A::GUS (909 pb) and pGmRD26B::GUS (435 bp), controlling the expression of the β-glucuronidase (uidA) gene. Analysis of GUS activity has demonstrated that pGmRD26 and pGmRD26A induce strong reporter gene expression, as the pAtRD29 positive control promoter under ABA and PEG treatment. Conclusions The full-length promoter pGmRD26 and the pGmRD26A module provides an improved uidA transcription capacity when compared with the other promoter module, especially in response to polyethylene glycol and drought treatments. These data indicate that pGmRD26A may become a promising biotechnological asset with potential use in the development of modified drought-tolerant plants or other plants designed for stress responses.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Stress-responsive promoter</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Drought tolerance</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Abscisic acid</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Promoter modules analysis</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Gene-promoter characterization</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Melo, Bruno P.</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Lourenço-Tessutti, Isabela T.</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Arraes, Fabrício B. 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Identification and characterization of the GmRD26 soybean promoter in response to abiotic stresses: potential tool for biotechnological application

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