1,3-propanediol production with Citrobacter werkmanii DSM17579: effect of a dhaD knock-out

Background 1,3-propanediol (PDO) is a substantially industrial metabolite used in the polymer industry. Although several natural PDO production hosts exist, e.g. Klebsiella sp., Citrobacter sp. and Clostridium sp., the PDO yield on glycerol is insufficient for an economically viable bio-process. Enh...
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Autor*in:	Maervoet, Veerle ET [verfasserIn] De Maeseneire, Sofie L Avci, Fatma G Beauprez, Joeri Soetaert, Wim K De Mey, Marjan

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2014

Schlagwörter:	DSM17579 ∆ Glycerol dehydrogenase 3-hydroxypropionaldehyde 1,3-propanediol Yield

Anmerkung:	© Maervoet et al.; licensee BioMed Central Ltd. 2014

Übergeordnetes Werk:	Enthalten in: Microbial cell factories - London : Biomed Central, 2002, 13(2014), 1 vom: 17. Mai
Übergeordnetes Werk:	volume:13 ; year:2014 ; number:1 ; day:17 ; month:05

Links:	Volltext

DOI / URN:	10.1186/1475-2859-13-70

Katalog-ID:	SPR028565266

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520			\|a Background 1,3-propanediol (PDO) is a substantially industrial metabolite used in the polymer industry. Although several natural PDO production hosts exist, e.g. Klebsiella sp., Citrobacter sp. and Clostridium sp., the PDO yield on glycerol is insufficient for an economically viable bio-process. Enhancing this yield via strain improvement can be achieved by disconnecting the production and growth pathways. In the case of PDO formation, this approach results in a microorganism metabolizing glycerol strictly for PDO production, while catabolizing a co-substrate for growth and maintenance. We applied this strategy to improve the PDO production with Citrobacter werkmanii DSM17579. Results Genetic tools were developed and used to create Citrobacter werkmanii DSM17579 ∆dhaD in which dhaD, encoding for glycerol dehydrogenase, was deleted. Since this strain was unable to grow on glycerol anaerobically, both pathways were disconnected. The knock-out strain was perturbed with 13 different co-substrates for growth and maintenance. Glucose was the most promising, although a competition between NADH-consuming enzymes and 1,3-propanediol dehydrogenase emerged. Conclusion Due to the deletion of dhaD in Citrobacter werkmanii DSM17579, the PDO production and growth pathway were split. As a consequence, the PDO yield on glycerol was improved 1,5 times, strengthening the idea that Citrobacter werkmanii DSM17579 could become an industrially interesting host for PDO production.
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allfields	10.1186/1475-2859-13-70 doi (DE-627)SPR028565266 (SPR)1475-2859-13-70-e DE-627 ger DE-627 rakwb eng Maervoet, Veerle ET verfasserin aut 1,3-propanediol production with Citrobacter werkmanii DSM17579: effect of a dhaD knock-out 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Maervoet et al.; licensee BioMed Central Ltd. 2014 Background 1,3-propanediol (PDO) is a substantially industrial metabolite used in the polymer industry. Although several natural PDO production hosts exist, e.g. Klebsiella sp., Citrobacter sp. and Clostridium sp., the PDO yield on glycerol is insufficient for an economically viable bio-process. Enhancing this yield via strain improvement can be achieved by disconnecting the production and growth pathways. In the case of PDO formation, this approach results in a microorganism metabolizing glycerol strictly for PDO production, while catabolizing a co-substrate for growth and maintenance. We applied this strategy to improve the PDO production with Citrobacter werkmanii DSM17579. Results Genetic tools were developed and used to create Citrobacter werkmanii DSM17579 ∆dhaD in which dhaD, encoding for glycerol dehydrogenase, was deleted. Since this strain was unable to grow on glycerol anaerobically, both pathways were disconnected. The knock-out strain was perturbed with 13 different co-substrates for growth and maintenance. Glucose was the most promising, although a competition between NADH-consuming enzymes and 1,3-propanediol dehydrogenase emerged. Conclusion Due to the deletion of dhaD in Citrobacter werkmanii DSM17579, the PDO production and growth pathway were split. As a consequence, the PDO yield on glycerol was improved 1,5 times, strengthening the idea that Citrobacter werkmanii DSM17579 could become an industrially interesting host for PDO production. DSM17579 ∆ (dpeaa)DE-He213 Glycerol dehydrogenase (dpeaa)DE-He213 3-hydroxypropionaldehyde (dpeaa)DE-He213 1,3-propanediol (dpeaa)DE-He213 Yield (dpeaa)DE-He213 De Maeseneire, Sofie L aut Avci, Fatma G aut Beauprez, Joeri aut Soetaert, Wim K aut De Mey, Marjan aut Enthalten in Microbial cell factories London : Biomed Central, 2002 13(2014), 1 vom: 17. Mai (DE-627)355987651 (DE-600)2091377-1 1475-2859 nnns volume:13 year:2014 number:1 day:17 month:05 https://dx.doi.org/10.1186/1475-2859-13-70 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2014 1 17 05
spelling	10.1186/1475-2859-13-70 doi (DE-627)SPR028565266 (SPR)1475-2859-13-70-e DE-627 ger DE-627 rakwb eng Maervoet, Veerle ET verfasserin aut 1,3-propanediol production with Citrobacter werkmanii DSM17579: effect of a dhaD knock-out 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Maervoet et al.; licensee BioMed Central Ltd. 2014 Background 1,3-propanediol (PDO) is a substantially industrial metabolite used in the polymer industry. Although several natural PDO production hosts exist, e.g. Klebsiella sp., Citrobacter sp. and Clostridium sp., the PDO yield on glycerol is insufficient for an economically viable bio-process. Enhancing this yield via strain improvement can be achieved by disconnecting the production and growth pathways. In the case of PDO formation, this approach results in a microorganism metabolizing glycerol strictly for PDO production, while catabolizing a co-substrate for growth and maintenance. We applied this strategy to improve the PDO production with Citrobacter werkmanii DSM17579. Results Genetic tools were developed and used to create Citrobacter werkmanii DSM17579 ∆dhaD in which dhaD, encoding for glycerol dehydrogenase, was deleted. Since this strain was unable to grow on glycerol anaerobically, both pathways were disconnected. The knock-out strain was perturbed with 13 different co-substrates for growth and maintenance. Glucose was the most promising, although a competition between NADH-consuming enzymes and 1,3-propanediol dehydrogenase emerged. Conclusion Due to the deletion of dhaD in Citrobacter werkmanii DSM17579, the PDO production and growth pathway were split. As a consequence, the PDO yield on glycerol was improved 1,5 times, strengthening the idea that Citrobacter werkmanii DSM17579 could become an industrially interesting host for PDO production. DSM17579 ∆ (dpeaa)DE-He213 Glycerol dehydrogenase (dpeaa)DE-He213 3-hydroxypropionaldehyde (dpeaa)DE-He213 1,3-propanediol (dpeaa)DE-He213 Yield (dpeaa)DE-He213 De Maeseneire, Sofie L aut Avci, Fatma G aut Beauprez, Joeri aut Soetaert, Wim K aut De Mey, Marjan aut Enthalten in Microbial cell factories London : Biomed Central, 2002 13(2014), 1 vom: 17. Mai (DE-627)355987651 (DE-600)2091377-1 1475-2859 nnns volume:13 year:2014 number:1 day:17 month:05 https://dx.doi.org/10.1186/1475-2859-13-70 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2014 1 17 05
allfields_unstemmed	10.1186/1475-2859-13-70 doi (DE-627)SPR028565266 (SPR)1475-2859-13-70-e DE-627 ger DE-627 rakwb eng Maervoet, Veerle ET verfasserin aut 1,3-propanediol production with Citrobacter werkmanii DSM17579: effect of a dhaD knock-out 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Maervoet et al.; licensee BioMed Central Ltd. 2014 Background 1,3-propanediol (PDO) is a substantially industrial metabolite used in the polymer industry. Although several natural PDO production hosts exist, e.g. Klebsiella sp., Citrobacter sp. and Clostridium sp., the PDO yield on glycerol is insufficient for an economically viable bio-process. Enhancing this yield via strain improvement can be achieved by disconnecting the production and growth pathways. In the case of PDO formation, this approach results in a microorganism metabolizing glycerol strictly for PDO production, while catabolizing a co-substrate for growth and maintenance. We applied this strategy to improve the PDO production with Citrobacter werkmanii DSM17579. Results Genetic tools were developed and used to create Citrobacter werkmanii DSM17579 ∆dhaD in which dhaD, encoding for glycerol dehydrogenase, was deleted. Since this strain was unable to grow on glycerol anaerobically, both pathways were disconnected. The knock-out strain was perturbed with 13 different co-substrates for growth and maintenance. Glucose was the most promising, although a competition between NADH-consuming enzymes and 1,3-propanediol dehydrogenase emerged. Conclusion Due to the deletion of dhaD in Citrobacter werkmanii DSM17579, the PDO production and growth pathway were split. As a consequence, the PDO yield on glycerol was improved 1,5 times, strengthening the idea that Citrobacter werkmanii DSM17579 could become an industrially interesting host for PDO production. DSM17579 ∆ (dpeaa)DE-He213 Glycerol dehydrogenase (dpeaa)DE-He213 3-hydroxypropionaldehyde (dpeaa)DE-He213 1,3-propanediol (dpeaa)DE-He213 Yield (dpeaa)DE-He213 De Maeseneire, Sofie L aut Avci, Fatma G aut Beauprez, Joeri aut Soetaert, Wim K aut De Mey, Marjan aut Enthalten in Microbial cell factories London : Biomed Central, 2002 13(2014), 1 vom: 17. Mai (DE-627)355987651 (DE-600)2091377-1 1475-2859 nnns volume:13 year:2014 number:1 day:17 month:05 https://dx.doi.org/10.1186/1475-2859-13-70 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2014 1 17 05
allfieldsGer	10.1186/1475-2859-13-70 doi (DE-627)SPR028565266 (SPR)1475-2859-13-70-e DE-627 ger DE-627 rakwb eng Maervoet, Veerle ET verfasserin aut 1,3-propanediol production with Citrobacter werkmanii DSM17579: effect of a dhaD knock-out 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Maervoet et al.; licensee BioMed Central Ltd. 2014 Background 1,3-propanediol (PDO) is a substantially industrial metabolite used in the polymer industry. Although several natural PDO production hosts exist, e.g. Klebsiella sp., Citrobacter sp. and Clostridium sp., the PDO yield on glycerol is insufficient for an economically viable bio-process. Enhancing this yield via strain improvement can be achieved by disconnecting the production and growth pathways. In the case of PDO formation, this approach results in a microorganism metabolizing glycerol strictly for PDO production, while catabolizing a co-substrate for growth and maintenance. We applied this strategy to improve the PDO production with Citrobacter werkmanii DSM17579. Results Genetic tools were developed and used to create Citrobacter werkmanii DSM17579 ∆dhaD in which dhaD, encoding for glycerol dehydrogenase, was deleted. Since this strain was unable to grow on glycerol anaerobically, both pathways were disconnected. The knock-out strain was perturbed with 13 different co-substrates for growth and maintenance. Glucose was the most promising, although a competition between NADH-consuming enzymes and 1,3-propanediol dehydrogenase emerged. Conclusion Due to the deletion of dhaD in Citrobacter werkmanii DSM17579, the PDO production and growth pathway were split. As a consequence, the PDO yield on glycerol was improved 1,5 times, strengthening the idea that Citrobacter werkmanii DSM17579 could become an industrially interesting host for PDO production. DSM17579 ∆ (dpeaa)DE-He213 Glycerol dehydrogenase (dpeaa)DE-He213 3-hydroxypropionaldehyde (dpeaa)DE-He213 1,3-propanediol (dpeaa)DE-He213 Yield (dpeaa)DE-He213 De Maeseneire, Sofie L aut Avci, Fatma G aut Beauprez, Joeri aut Soetaert, Wim K aut De Mey, Marjan aut Enthalten in Microbial cell factories London : Biomed Central, 2002 13(2014), 1 vom: 17. Mai (DE-627)355987651 (DE-600)2091377-1 1475-2859 nnns volume:13 year:2014 number:1 day:17 month:05 https://dx.doi.org/10.1186/1475-2859-13-70 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2014 1 17 05
allfieldsSound	10.1186/1475-2859-13-70 doi (DE-627)SPR028565266 (SPR)1475-2859-13-70-e DE-627 ger DE-627 rakwb eng Maervoet, Veerle ET verfasserin aut 1,3-propanediol production with Citrobacter werkmanii DSM17579: effect of a dhaD knock-out 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Maervoet et al.; licensee BioMed Central Ltd. 2014 Background 1,3-propanediol (PDO) is a substantially industrial metabolite used in the polymer industry. Although several natural PDO production hosts exist, e.g. Klebsiella sp., Citrobacter sp. and Clostridium sp., the PDO yield on glycerol is insufficient for an economically viable bio-process. Enhancing this yield via strain improvement can be achieved by disconnecting the production and growth pathways. In the case of PDO formation, this approach results in a microorganism metabolizing glycerol strictly for PDO production, while catabolizing a co-substrate for growth and maintenance. We applied this strategy to improve the PDO production with Citrobacter werkmanii DSM17579. Results Genetic tools were developed and used to create Citrobacter werkmanii DSM17579 ∆dhaD in which dhaD, encoding for glycerol dehydrogenase, was deleted. Since this strain was unable to grow on glycerol anaerobically, both pathways were disconnected. The knock-out strain was perturbed with 13 different co-substrates for growth and maintenance. Glucose was the most promising, although a competition between NADH-consuming enzymes and 1,3-propanediol dehydrogenase emerged. Conclusion Due to the deletion of dhaD in Citrobacter werkmanii DSM17579, the PDO production and growth pathway were split. As a consequence, the PDO yield on glycerol was improved 1,5 times, strengthening the idea that Citrobacter werkmanii DSM17579 could become an industrially interesting host for PDO production. DSM17579 ∆ (dpeaa)DE-He213 Glycerol dehydrogenase (dpeaa)DE-He213 3-hydroxypropionaldehyde (dpeaa)DE-He213 1,3-propanediol (dpeaa)DE-He213 Yield (dpeaa)DE-He213 De Maeseneire, Sofie L aut Avci, Fatma G aut Beauprez, Joeri aut Soetaert, Wim K aut De Mey, Marjan aut Enthalten in Microbial cell factories London : Biomed Central, 2002 13(2014), 1 vom: 17. Mai (DE-627)355987651 (DE-600)2091377-1 1475-2859 nnns volume:13 year:2014 number:1 day:17 month:05 https://dx.doi.org/10.1186/1475-2859-13-70 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2014 1 17 05
language	English
source	Enthalten in Microbial cell factories 13(2014), 1 vom: 17. Mai volume:13 year:2014 number:1 day:17 month:05
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topic_facet	DSM17579 ∆ Glycerol dehydrogenase 3-hydroxypropionaldehyde 1,3-propanediol Yield
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container_title	Microbial cell factories
authorswithroles_txt_mv	Maervoet, Veerle ET @@aut@@ De Maeseneire, Sofie L @@aut@@ Avci, Fatma G @@aut@@ Beauprez, Joeri @@aut@@ Soetaert, Wim K @@aut@@ De Mey, Marjan @@aut@@
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author	Maervoet, Veerle ET
spellingShingle	Maervoet, Veerle ET misc DSM17579 ∆ misc Glycerol dehydrogenase misc 3-hydroxypropionaldehyde misc 1,3-propanediol misc Yield 1,3-propanediol production with Citrobacter werkmanii DSM17579: effect of a dhaD knock-out
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topic_title	1,3-propanediol production with Citrobacter werkmanii DSM17579: effect of a dhaD knock-out DSM17579 ∆ (dpeaa)DE-He213 Glycerol dehydrogenase (dpeaa)DE-He213 3-hydroxypropionaldehyde (dpeaa)DE-He213 1,3-propanediol (dpeaa)DE-He213 Yield (dpeaa)DE-He213
topic	misc DSM17579 ∆ misc Glycerol dehydrogenase misc 3-hydroxypropionaldehyde misc 1,3-propanediol misc Yield
topic_unstemmed	misc DSM17579 ∆ misc Glycerol dehydrogenase misc 3-hydroxypropionaldehyde misc 1,3-propanediol misc Yield
topic_browse	misc DSM17579 ∆ misc Glycerol dehydrogenase misc 3-hydroxypropionaldehyde misc 1,3-propanediol misc Yield
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title	1,3-propanediol production with Citrobacter werkmanii DSM17579: effect of a dhaD knock-out
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title_full	1,3-propanediol production with Citrobacter werkmanii DSM17579: effect of a dhaD knock-out
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title_sort	1,3-propanediol production with citrobacter werkmanii dsm17579: effect of a dhad knock-out
title_auth	1,3-propanediol production with Citrobacter werkmanii DSM17579: effect of a dhaD knock-out
abstract	Background 1,3-propanediol (PDO) is a substantially industrial metabolite used in the polymer industry. Although several natural PDO production hosts exist, e.g. Klebsiella sp., Citrobacter sp. and Clostridium sp., the PDO yield on glycerol is insufficient for an economically viable bio-process. Enhancing this yield via strain improvement can be achieved by disconnecting the production and growth pathways. In the case of PDO formation, this approach results in a microorganism metabolizing glycerol strictly for PDO production, while catabolizing a co-substrate for growth and maintenance. We applied this strategy to improve the PDO production with Citrobacter werkmanii DSM17579. Results Genetic tools were developed and used to create Citrobacter werkmanii DSM17579 ∆dhaD in which dhaD, encoding for glycerol dehydrogenase, was deleted. Since this strain was unable to grow on glycerol anaerobically, both pathways were disconnected. The knock-out strain was perturbed with 13 different co-substrates for growth and maintenance. Glucose was the most promising, although a competition between NADH-consuming enzymes and 1,3-propanediol dehydrogenase emerged. Conclusion Due to the deletion of dhaD in Citrobacter werkmanii DSM17579, the PDO production and growth pathway were split. As a consequence, the PDO yield on glycerol was improved 1,5 times, strengthening the idea that Citrobacter werkmanii DSM17579 could become an industrially interesting host for PDO production. © Maervoet et al.; licensee BioMed Central Ltd. 2014
abstractGer	Background 1,3-propanediol (PDO) is a substantially industrial metabolite used in the polymer industry. Although several natural PDO production hosts exist, e.g. Klebsiella sp., Citrobacter sp. and Clostridium sp., the PDO yield on glycerol is insufficient for an economically viable bio-process. Enhancing this yield via strain improvement can be achieved by disconnecting the production and growth pathways. In the case of PDO formation, this approach results in a microorganism metabolizing glycerol strictly for PDO production, while catabolizing a co-substrate for growth and maintenance. We applied this strategy to improve the PDO production with Citrobacter werkmanii DSM17579. Results Genetic tools were developed and used to create Citrobacter werkmanii DSM17579 ∆dhaD in which dhaD, encoding for glycerol dehydrogenase, was deleted. Since this strain was unable to grow on glycerol anaerobically, both pathways were disconnected. The knock-out strain was perturbed with 13 different co-substrates for growth and maintenance. Glucose was the most promising, although a competition between NADH-consuming enzymes and 1,3-propanediol dehydrogenase emerged. Conclusion Due to the deletion of dhaD in Citrobacter werkmanii DSM17579, the PDO production and growth pathway were split. As a consequence, the PDO yield on glycerol was improved 1,5 times, strengthening the idea that Citrobacter werkmanii DSM17579 could become an industrially interesting host for PDO production. © Maervoet et al.; licensee BioMed Central Ltd. 2014
abstract_unstemmed	Background 1,3-propanediol (PDO) is a substantially industrial metabolite used in the polymer industry. Although several natural PDO production hosts exist, e.g. Klebsiella sp., Citrobacter sp. and Clostridium sp., the PDO yield on glycerol is insufficient for an economically viable bio-process. Enhancing this yield via strain improvement can be achieved by disconnecting the production and growth pathways. In the case of PDO formation, this approach results in a microorganism metabolizing glycerol strictly for PDO production, while catabolizing a co-substrate for growth and maintenance. We applied this strategy to improve the PDO production with Citrobacter werkmanii DSM17579. Results Genetic tools were developed and used to create Citrobacter werkmanii DSM17579 ∆dhaD in which dhaD, encoding for glycerol dehydrogenase, was deleted. Since this strain was unable to grow on glycerol anaerobically, both pathways were disconnected. The knock-out strain was perturbed with 13 different co-substrates for growth and maintenance. Glucose was the most promising, although a competition between NADH-consuming enzymes and 1,3-propanediol dehydrogenase emerged. Conclusion Due to the deletion of dhaD in Citrobacter werkmanii DSM17579, the PDO production and growth pathway were split. As a consequence, the PDO yield on glycerol was improved 1,5 times, strengthening the idea that Citrobacter werkmanii DSM17579 could become an industrially interesting host for PDO production. © Maervoet et al.; licensee BioMed Central Ltd. 2014
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title_short	1,3-propanediol production with Citrobacter werkmanii DSM17579: effect of a dhaD knock-out
url	https://dx.doi.org/10.1186/1475-2859-13-70
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author2	De Maeseneire, Sofie L Avci, Fatma G Beauprez, Joeri Soetaert, Wim K De Mey, Marjan
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up_date	2024-07-03T20:15:41.052Z
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Although several natural PDO production hosts exist, e.g. Klebsiella sp., Citrobacter sp. and Clostridium sp., the PDO yield on glycerol is insufficient for an economically viable bio-process. Enhancing this yield via strain improvement can be achieved by disconnecting the production and growth pathways. In the case of PDO formation, this approach results in a microorganism metabolizing glycerol strictly for PDO production, while catabolizing a co-substrate for growth and maintenance. We applied this strategy to improve the PDO production with Citrobacter werkmanii DSM17579. Results Genetic tools were developed and used to create Citrobacter werkmanii DSM17579 ∆dhaD in which dhaD, encoding for glycerol dehydrogenase, was deleted. Since this strain was unable to grow on glycerol anaerobically, both pathways were disconnected. The knock-out strain was perturbed with 13 different co-substrates for growth and maintenance. Glucose was the most promising, although a competition between NADH-consuming enzymes and 1,3-propanediol dehydrogenase emerged. Conclusion Due to the deletion of dhaD in Citrobacter werkmanii DSM17579, the PDO production and growth pathway were split. 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1,3-propanediol production with Citrobacter werkmanii DSM17579: effect of a dhaD knock-out

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