Recombinant human erythropoietin increases survival and reduces neuronal apoptosis in a murine model of cerebral malaria

Background Cerebral malaria (CM) is an acute encephalopathy with increased pro-inflammatory cytokines, sequestration of parasitized erythrocytes and localized ischaemia. In children CM induces cognitive impairment in about 10% of the survivors. Erythropoietin (Epo) has – besides of its well known ha...
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Autor*in:	Wiese, Lothar [verfasserIn] Hempel, Casper Penkowa, Milena Kirkby, Nikolai Kurtzhals, Jørgen AL

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2008

Schlagwörter:	Cerebral Malaria Packed Cell Volume Packed Cell Volume Level Eprex Plasmodium Berghei ANKA

Anmerkung:	© Wiese et al; licensee BioMed Central Ltd. 2008

Übergeordnetes Werk:	Enthalten in: Malaria journal - London : BioMed Central, 2002, 7(2008), 1 vom: 07. Jan.
Übergeordnetes Werk:	volume:7 ; year:2008 ; number:1 ; day:07 ; month:01

Links:	Volltext

DOI / URN:	10.1186/1475-2875-7-3

Katalog-ID:	SPR02860136X

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100	1		\|a Wiese, Lothar \|e verfasserin \|4 aut
245	1	0	\|a Recombinant human erythropoietin increases survival and reduces neuronal apoptosis in a murine model of cerebral malaria
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520			\|a Background Cerebral malaria (CM) is an acute encephalopathy with increased pro-inflammatory cytokines, sequestration of parasitized erythrocytes and localized ischaemia. In children CM induces cognitive impairment in about 10% of the survivors. Erythropoietin (Epo) has – besides of its well known haematopoietic properties – significant anti-inflammatory, antioxidant and anti-apoptotic effects in various brain disorders. The neurobiological responses to exogenously injected Epo during murine CM were examined. Methods Female C57BL/6j mice (4–6 weeks), infected with Plasmodium berghei ANKA, were treated with recombinant human Epo (rhEpo; 50–5000 U/kg/OD, i.p.) at different time points. The effect on survival was measured. Brain pathology was investigated by TUNEL (Terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphate (dUTP)-digoxigenin nick end labelling), as a marker of apoptosis. Gene expression in brain tissue was measured by real time PCR. Results Treatment with rhEpo increased survival in mice with CM in a dose- and time-dependent manner and reduced apoptotic cell death of neurons as well as the expression of pro-inflammatory cytokines in the brain. This neuroprotective effect appeared to be independent of the haematopoietic effect. Conclusion These results and its excellent safety profile in humans makes rhEpo a potential candidate for adjunct treatment of CM.
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Indexfelder

author_variant	l w lw c h ch m p mp n k nk j a k ja jak
matchkey_str	article:14752875:2008----::eobnnhmnrtrpitnnraesriaadeuenuoaaotssnm
hierarchy_sort_str	2008
publishDate	2008
allfields	10.1186/1475-2875-7-3 doi (DE-627)SPR02860136X (SPR)1475-2875-7-3-e DE-627 ger DE-627 rakwb eng Wiese, Lothar verfasserin aut Recombinant human erythropoietin increases survival and reduces neuronal apoptosis in a murine model of cerebral malaria 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Wiese et al; licensee BioMed Central Ltd. 2008 Background Cerebral malaria (CM) is an acute encephalopathy with increased pro-inflammatory cytokines, sequestration of parasitized erythrocytes and localized ischaemia. In children CM induces cognitive impairment in about 10% of the survivors. Erythropoietin (Epo) has – besides of its well known haematopoietic properties – significant anti-inflammatory, antioxidant and anti-apoptotic effects in various brain disorders. The neurobiological responses to exogenously injected Epo during murine CM were examined. Methods Female C57BL/6j mice (4–6 weeks), infected with Plasmodium berghei ANKA, were treated with recombinant human Epo (rhEpo; 50–5000 U/kg/OD, i.p.) at different time points. The effect on survival was measured. Brain pathology was investigated by TUNEL (Terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphate (dUTP)-digoxigenin nick end labelling), as a marker of apoptosis. Gene expression in brain tissue was measured by real time PCR. Results Treatment with rhEpo increased survival in mice with CM in a dose- and time-dependent manner and reduced apoptotic cell death of neurons as well as the expression of pro-inflammatory cytokines in the brain. This neuroprotective effect appeared to be independent of the haematopoietic effect. Conclusion These results and its excellent safety profile in humans makes rhEpo a potential candidate for adjunct treatment of CM. Cerebral Malaria (dpeaa)DE-He213 Packed Cell Volume (dpeaa)DE-He213 Packed Cell Volume Level (dpeaa)DE-He213 Eprex (dpeaa)DE-He213 Plasmodium Berghei ANKA (dpeaa)DE-He213 Hempel, Casper aut Penkowa, Milena aut Kirkby, Nikolai aut Kurtzhals, Jørgen AL aut Enthalten in Malaria journal London : BioMed Central, 2002 7(2008), 1 vom: 07. Jan. (DE-627)355986582 (DE-600)2091229-8 1475-2875 nnns volume:7 year:2008 number:1 day:07 month:01 https://dx.doi.org/10.1186/1475-2875-7-3 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2008 1 07 01
spelling	10.1186/1475-2875-7-3 doi (DE-627)SPR02860136X (SPR)1475-2875-7-3-e DE-627 ger DE-627 rakwb eng Wiese, Lothar verfasserin aut Recombinant human erythropoietin increases survival and reduces neuronal apoptosis in a murine model of cerebral malaria 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Wiese et al; licensee BioMed Central Ltd. 2008 Background Cerebral malaria (CM) is an acute encephalopathy with increased pro-inflammatory cytokines, sequestration of parasitized erythrocytes and localized ischaemia. In children CM induces cognitive impairment in about 10% of the survivors. Erythropoietin (Epo) has – besides of its well known haematopoietic properties – significant anti-inflammatory, antioxidant and anti-apoptotic effects in various brain disorders. The neurobiological responses to exogenously injected Epo during murine CM were examined. Methods Female C57BL/6j mice (4–6 weeks), infected with Plasmodium berghei ANKA, were treated with recombinant human Epo (rhEpo; 50–5000 U/kg/OD, i.p.) at different time points. The effect on survival was measured. Brain pathology was investigated by TUNEL (Terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphate (dUTP)-digoxigenin nick end labelling), as a marker of apoptosis. Gene expression in brain tissue was measured by real time PCR. Results Treatment with rhEpo increased survival in mice with CM in a dose- and time-dependent manner and reduced apoptotic cell death of neurons as well as the expression of pro-inflammatory cytokines in the brain. This neuroprotective effect appeared to be independent of the haematopoietic effect. Conclusion These results and its excellent safety profile in humans makes rhEpo a potential candidate for adjunct treatment of CM. Cerebral Malaria (dpeaa)DE-He213 Packed Cell Volume (dpeaa)DE-He213 Packed Cell Volume Level (dpeaa)DE-He213 Eprex (dpeaa)DE-He213 Plasmodium Berghei ANKA (dpeaa)DE-He213 Hempel, Casper aut Penkowa, Milena aut Kirkby, Nikolai aut Kurtzhals, Jørgen AL aut Enthalten in Malaria journal London : BioMed Central, 2002 7(2008), 1 vom: 07. Jan. (DE-627)355986582 (DE-600)2091229-8 1475-2875 nnns volume:7 year:2008 number:1 day:07 month:01 https://dx.doi.org/10.1186/1475-2875-7-3 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2008 1 07 01
allfields_unstemmed	10.1186/1475-2875-7-3 doi (DE-627)SPR02860136X (SPR)1475-2875-7-3-e DE-627 ger DE-627 rakwb eng Wiese, Lothar verfasserin aut Recombinant human erythropoietin increases survival and reduces neuronal apoptosis in a murine model of cerebral malaria 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Wiese et al; licensee BioMed Central Ltd. 2008 Background Cerebral malaria (CM) is an acute encephalopathy with increased pro-inflammatory cytokines, sequestration of parasitized erythrocytes and localized ischaemia. In children CM induces cognitive impairment in about 10% of the survivors. Erythropoietin (Epo) has – besides of its well known haematopoietic properties – significant anti-inflammatory, antioxidant and anti-apoptotic effects in various brain disorders. The neurobiological responses to exogenously injected Epo during murine CM were examined. Methods Female C57BL/6j mice (4–6 weeks), infected with Plasmodium berghei ANKA, were treated with recombinant human Epo (rhEpo; 50–5000 U/kg/OD, i.p.) at different time points. The effect on survival was measured. Brain pathology was investigated by TUNEL (Terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphate (dUTP)-digoxigenin nick end labelling), as a marker of apoptosis. Gene expression in brain tissue was measured by real time PCR. Results Treatment with rhEpo increased survival in mice with CM in a dose- and time-dependent manner and reduced apoptotic cell death of neurons as well as the expression of pro-inflammatory cytokines in the brain. This neuroprotective effect appeared to be independent of the haematopoietic effect. Conclusion These results and its excellent safety profile in humans makes rhEpo a potential candidate for adjunct treatment of CM. Cerebral Malaria (dpeaa)DE-He213 Packed Cell Volume (dpeaa)DE-He213 Packed Cell Volume Level (dpeaa)DE-He213 Eprex (dpeaa)DE-He213 Plasmodium Berghei ANKA (dpeaa)DE-He213 Hempel, Casper aut Penkowa, Milena aut Kirkby, Nikolai aut Kurtzhals, Jørgen AL aut Enthalten in Malaria journal London : BioMed Central, 2002 7(2008), 1 vom: 07. Jan. (DE-627)355986582 (DE-600)2091229-8 1475-2875 nnns volume:7 year:2008 number:1 day:07 month:01 https://dx.doi.org/10.1186/1475-2875-7-3 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2008 1 07 01
allfieldsGer	10.1186/1475-2875-7-3 doi (DE-627)SPR02860136X (SPR)1475-2875-7-3-e DE-627 ger DE-627 rakwb eng Wiese, Lothar verfasserin aut Recombinant human erythropoietin increases survival and reduces neuronal apoptosis in a murine model of cerebral malaria 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Wiese et al; licensee BioMed Central Ltd. 2008 Background Cerebral malaria (CM) is an acute encephalopathy with increased pro-inflammatory cytokines, sequestration of parasitized erythrocytes and localized ischaemia. In children CM induces cognitive impairment in about 10% of the survivors. Erythropoietin (Epo) has – besides of its well known haematopoietic properties – significant anti-inflammatory, antioxidant and anti-apoptotic effects in various brain disorders. The neurobiological responses to exogenously injected Epo during murine CM were examined. Methods Female C57BL/6j mice (4–6 weeks), infected with Plasmodium berghei ANKA, were treated with recombinant human Epo (rhEpo; 50–5000 U/kg/OD, i.p.) at different time points. The effect on survival was measured. Brain pathology was investigated by TUNEL (Terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphate (dUTP)-digoxigenin nick end labelling), as a marker of apoptosis. Gene expression in brain tissue was measured by real time PCR. Results Treatment with rhEpo increased survival in mice with CM in a dose- and time-dependent manner and reduced apoptotic cell death of neurons as well as the expression of pro-inflammatory cytokines in the brain. This neuroprotective effect appeared to be independent of the haematopoietic effect. Conclusion These results and its excellent safety profile in humans makes rhEpo a potential candidate for adjunct treatment of CM. Cerebral Malaria (dpeaa)DE-He213 Packed Cell Volume (dpeaa)DE-He213 Packed Cell Volume Level (dpeaa)DE-He213 Eprex (dpeaa)DE-He213 Plasmodium Berghei ANKA (dpeaa)DE-He213 Hempel, Casper aut Penkowa, Milena aut Kirkby, Nikolai aut Kurtzhals, Jørgen AL aut Enthalten in Malaria journal London : BioMed Central, 2002 7(2008), 1 vom: 07. Jan. (DE-627)355986582 (DE-600)2091229-8 1475-2875 nnns volume:7 year:2008 number:1 day:07 month:01 https://dx.doi.org/10.1186/1475-2875-7-3 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2008 1 07 01
allfieldsSound	10.1186/1475-2875-7-3 doi (DE-627)SPR02860136X (SPR)1475-2875-7-3-e DE-627 ger DE-627 rakwb eng Wiese, Lothar verfasserin aut Recombinant human erythropoietin increases survival and reduces neuronal apoptosis in a murine model of cerebral malaria 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Wiese et al; licensee BioMed Central Ltd. 2008 Background Cerebral malaria (CM) is an acute encephalopathy with increased pro-inflammatory cytokines, sequestration of parasitized erythrocytes and localized ischaemia. In children CM induces cognitive impairment in about 10% of the survivors. Erythropoietin (Epo) has – besides of its well known haematopoietic properties – significant anti-inflammatory, antioxidant and anti-apoptotic effects in various brain disorders. The neurobiological responses to exogenously injected Epo during murine CM were examined. Methods Female C57BL/6j mice (4–6 weeks), infected with Plasmodium berghei ANKA, were treated with recombinant human Epo (rhEpo; 50–5000 U/kg/OD, i.p.) at different time points. The effect on survival was measured. Brain pathology was investigated by TUNEL (Terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphate (dUTP)-digoxigenin nick end labelling), as a marker of apoptosis. Gene expression in brain tissue was measured by real time PCR. Results Treatment with rhEpo increased survival in mice with CM in a dose- and time-dependent manner and reduced apoptotic cell death of neurons as well as the expression of pro-inflammatory cytokines in the brain. This neuroprotective effect appeared to be independent of the haematopoietic effect. Conclusion These results and its excellent safety profile in humans makes rhEpo a potential candidate for adjunct treatment of CM. Cerebral Malaria (dpeaa)DE-He213 Packed Cell Volume (dpeaa)DE-He213 Packed Cell Volume Level (dpeaa)DE-He213 Eprex (dpeaa)DE-He213 Plasmodium Berghei ANKA (dpeaa)DE-He213 Hempel, Casper aut Penkowa, Milena aut Kirkby, Nikolai aut Kurtzhals, Jørgen AL aut Enthalten in Malaria journal London : BioMed Central, 2002 7(2008), 1 vom: 07. Jan. (DE-627)355986582 (DE-600)2091229-8 1475-2875 nnns volume:7 year:2008 number:1 day:07 month:01 https://dx.doi.org/10.1186/1475-2875-7-3 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2008 1 07 01
language	English
source	Enthalten in Malaria journal 7(2008), 1 vom: 07. Jan. volume:7 year:2008 number:1 day:07 month:01
sourceStr	Enthalten in Malaria journal 7(2008), 1 vom: 07. Jan. volume:7 year:2008 number:1 day:07 month:01
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Cerebral Malaria Packed Cell Volume Packed Cell Volume Level Eprex Plasmodium Berghei ANKA
isfreeaccess_bool	true
container_title	Malaria journal
authorswithroles_txt_mv	Wiese, Lothar @@aut@@ Hempel, Casper @@aut@@ Penkowa, Milena @@aut@@ Kirkby, Nikolai @@aut@@ Kurtzhals, Jørgen AL @@aut@@
publishDateDaySort_date	2008-01-07T00:00:00Z
hierarchy_top_id	355986582
id	SPR02860136X
language_de	englisch
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author	Wiese, Lothar
spellingShingle	Wiese, Lothar misc Cerebral Malaria misc Packed Cell Volume misc Packed Cell Volume Level misc Eprex misc Plasmodium Berghei ANKA Recombinant human erythropoietin increases survival and reduces neuronal apoptosis in a murine model of cerebral malaria
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topic_title	Recombinant human erythropoietin increases survival and reduces neuronal apoptosis in a murine model of cerebral malaria Cerebral Malaria (dpeaa)DE-He213 Packed Cell Volume (dpeaa)DE-He213 Packed Cell Volume Level (dpeaa)DE-He213 Eprex (dpeaa)DE-He213 Plasmodium Berghei ANKA (dpeaa)DE-He213
topic	misc Cerebral Malaria misc Packed Cell Volume misc Packed Cell Volume Level misc Eprex misc Plasmodium Berghei ANKA
topic_unstemmed	misc Cerebral Malaria misc Packed Cell Volume misc Packed Cell Volume Level misc Eprex misc Plasmodium Berghei ANKA
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title	Recombinant human erythropoietin increases survival and reduces neuronal apoptosis in a murine model of cerebral malaria
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title_full	Recombinant human erythropoietin increases survival and reduces neuronal apoptosis in a murine model of cerebral malaria
author_sort	Wiese, Lothar
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author_browse	Wiese, Lothar Hempel, Casper Penkowa, Milena Kirkby, Nikolai Kurtzhals, Jørgen AL
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title_sort	recombinant human erythropoietin increases survival and reduces neuronal apoptosis in a murine model of cerebral malaria
title_auth	Recombinant human erythropoietin increases survival and reduces neuronal apoptosis in a murine model of cerebral malaria
abstract	Background Cerebral malaria (CM) is an acute encephalopathy with increased pro-inflammatory cytokines, sequestration of parasitized erythrocytes and localized ischaemia. In children CM induces cognitive impairment in about 10% of the survivors. Erythropoietin (Epo) has – besides of its well known haematopoietic properties – significant anti-inflammatory, antioxidant and anti-apoptotic effects in various brain disorders. The neurobiological responses to exogenously injected Epo during murine CM were examined. Methods Female C57BL/6j mice (4–6 weeks), infected with Plasmodium berghei ANKA, were treated with recombinant human Epo (rhEpo; 50–5000 U/kg/OD, i.p.) at different time points. The effect on survival was measured. Brain pathology was investigated by TUNEL (Terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphate (dUTP)-digoxigenin nick end labelling), as a marker of apoptosis. Gene expression in brain tissue was measured by real time PCR. Results Treatment with rhEpo increased survival in mice with CM in a dose- and time-dependent manner and reduced apoptotic cell death of neurons as well as the expression of pro-inflammatory cytokines in the brain. This neuroprotective effect appeared to be independent of the haematopoietic effect. Conclusion These results and its excellent safety profile in humans makes rhEpo a potential candidate for adjunct treatment of CM. © Wiese et al; licensee BioMed Central Ltd. 2008
abstractGer	Background Cerebral malaria (CM) is an acute encephalopathy with increased pro-inflammatory cytokines, sequestration of parasitized erythrocytes and localized ischaemia. In children CM induces cognitive impairment in about 10% of the survivors. Erythropoietin (Epo) has – besides of its well known haematopoietic properties – significant anti-inflammatory, antioxidant and anti-apoptotic effects in various brain disorders. The neurobiological responses to exogenously injected Epo during murine CM were examined. Methods Female C57BL/6j mice (4–6 weeks), infected with Plasmodium berghei ANKA, were treated with recombinant human Epo (rhEpo; 50–5000 U/kg/OD, i.p.) at different time points. The effect on survival was measured. Brain pathology was investigated by TUNEL (Terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphate (dUTP)-digoxigenin nick end labelling), as a marker of apoptosis. Gene expression in brain tissue was measured by real time PCR. Results Treatment with rhEpo increased survival in mice with CM in a dose- and time-dependent manner and reduced apoptotic cell death of neurons as well as the expression of pro-inflammatory cytokines in the brain. This neuroprotective effect appeared to be independent of the haematopoietic effect. Conclusion These results and its excellent safety profile in humans makes rhEpo a potential candidate for adjunct treatment of CM. © Wiese et al; licensee BioMed Central Ltd. 2008
abstract_unstemmed	Background Cerebral malaria (CM) is an acute encephalopathy with increased pro-inflammatory cytokines, sequestration of parasitized erythrocytes and localized ischaemia. In children CM induces cognitive impairment in about 10% of the survivors. Erythropoietin (Epo) has – besides of its well known haematopoietic properties – significant anti-inflammatory, antioxidant and anti-apoptotic effects in various brain disorders. The neurobiological responses to exogenously injected Epo during murine CM were examined. Methods Female C57BL/6j mice (4–6 weeks), infected with Plasmodium berghei ANKA, were treated with recombinant human Epo (rhEpo; 50–5000 U/kg/OD, i.p.) at different time points. The effect on survival was measured. Brain pathology was investigated by TUNEL (Terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphate (dUTP)-digoxigenin nick end labelling), as a marker of apoptosis. Gene expression in brain tissue was measured by real time PCR. Results Treatment with rhEpo increased survival in mice with CM in a dose- and time-dependent manner and reduced apoptotic cell death of neurons as well as the expression of pro-inflammatory cytokines in the brain. This neuroprotective effect appeared to be independent of the haematopoietic effect. Conclusion These results and its excellent safety profile in humans makes rhEpo a potential candidate for adjunct treatment of CM. © Wiese et al; licensee BioMed Central Ltd. 2008
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title_short	Recombinant human erythropoietin increases survival and reduces neuronal apoptosis in a murine model of cerebral malaria
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Recombinant human erythropoietin increases survival and reduces neuronal apoptosis in a murine model of cerebral malaria

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