Evaluation of immunity against malaria using luciferase-expressing Plasmodium berghei parasites
Background Measurement of liver stage development is of key interest in malaria biology and vaccine studies. Parasite development in liver cells can be visualized in real-time, both in culture and in live mice, using a transgenic Plasmodium berghei parasite, Pb GFP-$ Luc_{con} $, expressing the biol...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Ploemen, Ivo [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2011 |
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© Ploemen et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( |
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| Übergeordnetes Werk: |
Enthalten in: Malaria journal - London : BioMed Central, 2002, 10(2011), 1 vom: 09. Dez. |
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| Übergeordnetes Werk: |
volume:10 ; year:2011 ; number:1 ; day:09 ; month:12 |
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| DOI / URN: |
10.1186/1475-2875-10-350 |
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| Katalog-ID: |
SPR028617746 |
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| 520 | |a Background Measurement of liver stage development is of key interest in malaria biology and vaccine studies. Parasite development in liver cells can be visualized in real-time, both in culture and in live mice, using a transgenic Plasmodium berghei parasite, Pb GFP-$ Luc_{con} $, expressing the bioluminescent reporter luciferase. This study explores the benefit of using these parasites for the evaluation of immunity against malaria, compared to qRT-PCR techniques in vivo and in vitro. Methods Mice were immunized with either radiation attenuated sporozoites (RAS) or wildtype sporozoites under chloroquine prophylaxis (CPS) and challenged with Pb GFP-$ Luc_{con.} $ The in vitro transgenic sporozoites neutralization assay (TSNA) was adapted by replacing Pb CS(Pf) parasites for Pb GFP-$ Luc_{con} $ parasites. Results Application of Pb GFP-$ Luc_{con} $ transgenic parasites provides live quantitative visual information about the relation between parasite liver load and protection. Moreover, fast and reproducible results are obtained by using these parasites in the transgenic sporozoites neutralization assay, measuring functional antibody-mediated immune responses. Conclusions Pb GFP-$ Luc_{con} $ parasites are a straightforward and valuable tool for comprehension of the biological and immunological principles underlying protection against malaria. | ||
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| 700 | 1 | |a Bijker, Else |4 aut | |
| 700 | 1 | |a Hermsen, Cornelus |4 aut | |
| 700 | 1 | |a Sauerwein, Robert |4 aut | |
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10.1186/1475-2875-10-350 doi (DE-627)SPR028617746 (SPR)1475-2875-10-350-e DE-627 ger DE-627 rakwb eng Ploemen, Ivo verfasserin aut Evaluation of immunity against malaria using luciferase-expressing Plasmodium berghei parasites 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Ploemen et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( Background Measurement of liver stage development is of key interest in malaria biology and vaccine studies. Parasite development in liver cells can be visualized in real-time, both in culture and in live mice, using a transgenic Plasmodium berghei parasite, Pb GFP-$ Luc_{con} $, expressing the bioluminescent reporter luciferase. This study explores the benefit of using these parasites for the evaluation of immunity against malaria, compared to qRT-PCR techniques in vivo and in vitro. Methods Mice were immunized with either radiation attenuated sporozoites (RAS) or wildtype sporozoites under chloroquine prophylaxis (CPS) and challenged with Pb GFP-$ Luc_{con.} $ The in vitro transgenic sporozoites neutralization assay (TSNA) was adapted by replacing Pb CS(Pf) parasites for Pb GFP-$ Luc_{con} $ parasites. Results Application of Pb GFP-$ Luc_{con} $ transgenic parasites provides live quantitative visual information about the relation between parasite liver load and protection. Moreover, fast and reproducible results are obtained by using these parasites in the transgenic sporozoites neutralization assay, measuring functional antibody-mediated immune responses. Conclusions Pb GFP-$ Luc_{con} $ parasites are a straightforward and valuable tool for comprehension of the biological and immunological principles underlying protection against malaria. Malaria (dpeaa)DE-He213 Bioluminescent Imaging (dpeaa)DE-He213 Caliper Life (dpeaa)DE-He213 Bioluminescent Intensity (dpeaa)DE-He213 Transgenic Parasite (dpeaa)DE-He213 Behet, Marije aut Nganou-Makamdop, Krystelle aut van Gemert, Geert-Jan aut Bijker, Else aut Hermsen, Cornelus aut Sauerwein, Robert aut Enthalten in Malaria journal London : BioMed Central, 2002 10(2011), 1 vom: 09. Dez. (DE-627)355986582 (DE-600)2091229-8 1475-2875 nnns volume:10 year:2011 number:1 day:09 month:12 https://dx.doi.org/10.1186/1475-2875-10-350 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2011 1 09 12 |
| spelling |
10.1186/1475-2875-10-350 doi (DE-627)SPR028617746 (SPR)1475-2875-10-350-e DE-627 ger DE-627 rakwb eng Ploemen, Ivo verfasserin aut Evaluation of immunity against malaria using luciferase-expressing Plasmodium berghei parasites 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Ploemen et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( Background Measurement of liver stage development is of key interest in malaria biology and vaccine studies. Parasite development in liver cells can be visualized in real-time, both in culture and in live mice, using a transgenic Plasmodium berghei parasite, Pb GFP-$ Luc_{con} $, expressing the bioluminescent reporter luciferase. This study explores the benefit of using these parasites for the evaluation of immunity against malaria, compared to qRT-PCR techniques in vivo and in vitro. Methods Mice were immunized with either radiation attenuated sporozoites (RAS) or wildtype sporozoites under chloroquine prophylaxis (CPS) and challenged with Pb GFP-$ Luc_{con.} $ The in vitro transgenic sporozoites neutralization assay (TSNA) was adapted by replacing Pb CS(Pf) parasites for Pb GFP-$ Luc_{con} $ parasites. Results Application of Pb GFP-$ Luc_{con} $ transgenic parasites provides live quantitative visual information about the relation between parasite liver load and protection. Moreover, fast and reproducible results are obtained by using these parasites in the transgenic sporozoites neutralization assay, measuring functional antibody-mediated immune responses. Conclusions Pb GFP-$ Luc_{con} $ parasites are a straightforward and valuable tool for comprehension of the biological and immunological principles underlying protection against malaria. Malaria (dpeaa)DE-He213 Bioluminescent Imaging (dpeaa)DE-He213 Caliper Life (dpeaa)DE-He213 Bioluminescent Intensity (dpeaa)DE-He213 Transgenic Parasite (dpeaa)DE-He213 Behet, Marije aut Nganou-Makamdop, Krystelle aut van Gemert, Geert-Jan aut Bijker, Else aut Hermsen, Cornelus aut Sauerwein, Robert aut Enthalten in Malaria journal London : BioMed Central, 2002 10(2011), 1 vom: 09. Dez. (DE-627)355986582 (DE-600)2091229-8 1475-2875 nnns volume:10 year:2011 number:1 day:09 month:12 https://dx.doi.org/10.1186/1475-2875-10-350 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2011 1 09 12 |
| allfields_unstemmed |
10.1186/1475-2875-10-350 doi (DE-627)SPR028617746 (SPR)1475-2875-10-350-e DE-627 ger DE-627 rakwb eng Ploemen, Ivo verfasserin aut Evaluation of immunity against malaria using luciferase-expressing Plasmodium berghei parasites 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Ploemen et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( Background Measurement of liver stage development is of key interest in malaria biology and vaccine studies. Parasite development in liver cells can be visualized in real-time, both in culture and in live mice, using a transgenic Plasmodium berghei parasite, Pb GFP-$ Luc_{con} $, expressing the bioluminescent reporter luciferase. This study explores the benefit of using these parasites for the evaluation of immunity against malaria, compared to qRT-PCR techniques in vivo and in vitro. Methods Mice were immunized with either radiation attenuated sporozoites (RAS) or wildtype sporozoites under chloroquine prophylaxis (CPS) and challenged with Pb GFP-$ Luc_{con.} $ The in vitro transgenic sporozoites neutralization assay (TSNA) was adapted by replacing Pb CS(Pf) parasites for Pb GFP-$ Luc_{con} $ parasites. Results Application of Pb GFP-$ Luc_{con} $ transgenic parasites provides live quantitative visual information about the relation between parasite liver load and protection. Moreover, fast and reproducible results are obtained by using these parasites in the transgenic sporozoites neutralization assay, measuring functional antibody-mediated immune responses. Conclusions Pb GFP-$ Luc_{con} $ parasites are a straightforward and valuable tool for comprehension of the biological and immunological principles underlying protection against malaria. Malaria (dpeaa)DE-He213 Bioluminescent Imaging (dpeaa)DE-He213 Caliper Life (dpeaa)DE-He213 Bioluminescent Intensity (dpeaa)DE-He213 Transgenic Parasite (dpeaa)DE-He213 Behet, Marije aut Nganou-Makamdop, Krystelle aut van Gemert, Geert-Jan aut Bijker, Else aut Hermsen, Cornelus aut Sauerwein, Robert aut Enthalten in Malaria journal London : BioMed Central, 2002 10(2011), 1 vom: 09. Dez. (DE-627)355986582 (DE-600)2091229-8 1475-2875 nnns volume:10 year:2011 number:1 day:09 month:12 https://dx.doi.org/10.1186/1475-2875-10-350 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2011 1 09 12 |
| allfieldsGer |
10.1186/1475-2875-10-350 doi (DE-627)SPR028617746 (SPR)1475-2875-10-350-e DE-627 ger DE-627 rakwb eng Ploemen, Ivo verfasserin aut Evaluation of immunity against malaria using luciferase-expressing Plasmodium berghei parasites 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Ploemen et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( Background Measurement of liver stage development is of key interest in malaria biology and vaccine studies. Parasite development in liver cells can be visualized in real-time, both in culture and in live mice, using a transgenic Plasmodium berghei parasite, Pb GFP-$ Luc_{con} $, expressing the bioluminescent reporter luciferase. This study explores the benefit of using these parasites for the evaluation of immunity against malaria, compared to qRT-PCR techniques in vivo and in vitro. Methods Mice were immunized with either radiation attenuated sporozoites (RAS) or wildtype sporozoites under chloroquine prophylaxis (CPS) and challenged with Pb GFP-$ Luc_{con.} $ The in vitro transgenic sporozoites neutralization assay (TSNA) was adapted by replacing Pb CS(Pf) parasites for Pb GFP-$ Luc_{con} $ parasites. Results Application of Pb GFP-$ Luc_{con} $ transgenic parasites provides live quantitative visual information about the relation between parasite liver load and protection. Moreover, fast and reproducible results are obtained by using these parasites in the transgenic sporozoites neutralization assay, measuring functional antibody-mediated immune responses. Conclusions Pb GFP-$ Luc_{con} $ parasites are a straightforward and valuable tool for comprehension of the biological and immunological principles underlying protection against malaria. Malaria (dpeaa)DE-He213 Bioluminescent Imaging (dpeaa)DE-He213 Caliper Life (dpeaa)DE-He213 Bioluminescent Intensity (dpeaa)DE-He213 Transgenic Parasite (dpeaa)DE-He213 Behet, Marije aut Nganou-Makamdop, Krystelle aut van Gemert, Geert-Jan aut Bijker, Else aut Hermsen, Cornelus aut Sauerwein, Robert aut Enthalten in Malaria journal London : BioMed Central, 2002 10(2011), 1 vom: 09. Dez. (DE-627)355986582 (DE-600)2091229-8 1475-2875 nnns volume:10 year:2011 number:1 day:09 month:12 https://dx.doi.org/10.1186/1475-2875-10-350 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2011 1 09 12 |
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10.1186/1475-2875-10-350 doi (DE-627)SPR028617746 (SPR)1475-2875-10-350-e DE-627 ger DE-627 rakwb eng Ploemen, Ivo verfasserin aut Evaluation of immunity against malaria using luciferase-expressing Plasmodium berghei parasites 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Ploemen et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( Background Measurement of liver stage development is of key interest in malaria biology and vaccine studies. Parasite development in liver cells can be visualized in real-time, both in culture and in live mice, using a transgenic Plasmodium berghei parasite, Pb GFP-$ Luc_{con} $, expressing the bioluminescent reporter luciferase. This study explores the benefit of using these parasites for the evaluation of immunity against malaria, compared to qRT-PCR techniques in vivo and in vitro. Methods Mice were immunized with either radiation attenuated sporozoites (RAS) or wildtype sporozoites under chloroquine prophylaxis (CPS) and challenged with Pb GFP-$ Luc_{con.} $ The in vitro transgenic sporozoites neutralization assay (TSNA) was adapted by replacing Pb CS(Pf) parasites for Pb GFP-$ Luc_{con} $ parasites. Results Application of Pb GFP-$ Luc_{con} $ transgenic parasites provides live quantitative visual information about the relation between parasite liver load and protection. Moreover, fast and reproducible results are obtained by using these parasites in the transgenic sporozoites neutralization assay, measuring functional antibody-mediated immune responses. Conclusions Pb GFP-$ Luc_{con} $ parasites are a straightforward and valuable tool for comprehension of the biological and immunological principles underlying protection against malaria. Malaria (dpeaa)DE-He213 Bioluminescent Imaging (dpeaa)DE-He213 Caliper Life (dpeaa)DE-He213 Bioluminescent Intensity (dpeaa)DE-He213 Transgenic Parasite (dpeaa)DE-He213 Behet, Marije aut Nganou-Makamdop, Krystelle aut van Gemert, Geert-Jan aut Bijker, Else aut Hermsen, Cornelus aut Sauerwein, Robert aut Enthalten in Malaria journal London : BioMed Central, 2002 10(2011), 1 vom: 09. Dez. (DE-627)355986582 (DE-600)2091229-8 1475-2875 nnns volume:10 year:2011 number:1 day:09 month:12 https://dx.doi.org/10.1186/1475-2875-10-350 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2011 1 09 12 |
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10.1186/1475-2875-10-350 |
| title_sort |
evaluation of immunity against malaria using luciferase-expressing plasmodium berghei parasites |
| title_auth |
Evaluation of immunity against malaria using luciferase-expressing Plasmodium berghei parasites |
| abstract |
Background Measurement of liver stage development is of key interest in malaria biology and vaccine studies. Parasite development in liver cells can be visualized in real-time, both in culture and in live mice, using a transgenic Plasmodium berghei parasite, Pb GFP-$ Luc_{con} $, expressing the bioluminescent reporter luciferase. This study explores the benefit of using these parasites for the evaluation of immunity against malaria, compared to qRT-PCR techniques in vivo and in vitro. Methods Mice were immunized with either radiation attenuated sporozoites (RAS) or wildtype sporozoites under chloroquine prophylaxis (CPS) and challenged with Pb GFP-$ Luc_{con.} $ The in vitro transgenic sporozoites neutralization assay (TSNA) was adapted by replacing Pb CS(Pf) parasites for Pb GFP-$ Luc_{con} $ parasites. Results Application of Pb GFP-$ Luc_{con} $ transgenic parasites provides live quantitative visual information about the relation between parasite liver load and protection. Moreover, fast and reproducible results are obtained by using these parasites in the transgenic sporozoites neutralization assay, measuring functional antibody-mediated immune responses. Conclusions Pb GFP-$ Luc_{con} $ parasites are a straightforward and valuable tool for comprehension of the biological and immunological principles underlying protection against malaria. © Ploemen et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( |
| abstractGer |
Background Measurement of liver stage development is of key interest in malaria biology and vaccine studies. Parasite development in liver cells can be visualized in real-time, both in culture and in live mice, using a transgenic Plasmodium berghei parasite, Pb GFP-$ Luc_{con} $, expressing the bioluminescent reporter luciferase. This study explores the benefit of using these parasites for the evaluation of immunity against malaria, compared to qRT-PCR techniques in vivo and in vitro. Methods Mice were immunized with either radiation attenuated sporozoites (RAS) or wildtype sporozoites under chloroquine prophylaxis (CPS) and challenged with Pb GFP-$ Luc_{con.} $ The in vitro transgenic sporozoites neutralization assay (TSNA) was adapted by replacing Pb CS(Pf) parasites for Pb GFP-$ Luc_{con} $ parasites. Results Application of Pb GFP-$ Luc_{con} $ transgenic parasites provides live quantitative visual information about the relation between parasite liver load and protection. Moreover, fast and reproducible results are obtained by using these parasites in the transgenic sporozoites neutralization assay, measuring functional antibody-mediated immune responses. Conclusions Pb GFP-$ Luc_{con} $ parasites are a straightforward and valuable tool for comprehension of the biological and immunological principles underlying protection against malaria. © Ploemen et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( |
| abstract_unstemmed |
Background Measurement of liver stage development is of key interest in malaria biology and vaccine studies. Parasite development in liver cells can be visualized in real-time, both in culture and in live mice, using a transgenic Plasmodium berghei parasite, Pb GFP-$ Luc_{con} $, expressing the bioluminescent reporter luciferase. This study explores the benefit of using these parasites for the evaluation of immunity against malaria, compared to qRT-PCR techniques in vivo and in vitro. Methods Mice were immunized with either radiation attenuated sporozoites (RAS) or wildtype sporozoites under chloroquine prophylaxis (CPS) and challenged with Pb GFP-$ Luc_{con.} $ The in vitro transgenic sporozoites neutralization assay (TSNA) was adapted by replacing Pb CS(Pf) parasites for Pb GFP-$ Luc_{con} $ parasites. Results Application of Pb GFP-$ Luc_{con} $ transgenic parasites provides live quantitative visual information about the relation between parasite liver load and protection. Moreover, fast and reproducible results are obtained by using these parasites in the transgenic sporozoites neutralization assay, measuring functional antibody-mediated immune responses. Conclusions Pb GFP-$ Luc_{con} $ parasites are a straightforward and valuable tool for comprehension of the biological and immunological principles underlying protection against malaria. © Ploemen et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( |
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Evaluation of immunity against malaria using luciferase-expressing Plasmodium berghei parasites |
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Behet, Marije Nganou-Makamdop, Krystelle van Gemert, Geert-Jan Bijker, Else Hermsen, Cornelus Sauerwein, Robert |
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