124I-$ HuCC49deltaC_{H} $2 for TAG-72 antigen-directed positron emission tomography (PET) imaging of LS174T colon adenocarcinoma tumor implants in xenograft mice: preliminary results
Background 18F-fluorodeoxyglucose positron emission tomography (18F-FDG-PET) is widely used in diagnostic cancer imaging. However, the use of 18F-FDG in PET-based imaging is limited by its specificity and sensitivity. In contrast, anti-TAG (tumor associated glycoprotein)-72 monoclonal antibodies are...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Zou, Peng [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2010 |
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| Anmerkung: |
© Zou et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( |
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| Übergeordnetes Werk: |
Enthalten in: World journal of surgical oncology - London : Biomed Central, 2003, 8(2010), 1 vom: 06. Aug. |
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| Übergeordnetes Werk: |
volume:8 ; year:2010 ; number:1 ; day:06 ; month:08 |
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| DOI / URN: |
10.1186/1477-7819-8-65 |
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| Katalog-ID: |
SPR028816021 |
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| 100 | 1 | |a Zou, Peng |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a 124I-$ HuCC49deltaC_{H} $2 for TAG-72 antigen-directed positron emission tomography (PET) imaging of LS174T colon adenocarcinoma tumor implants in xenograft mice: preliminary results |
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| 520 | |a Background 18F-fluorodeoxyglucose positron emission tomography (18F-FDG-PET) is widely used in diagnostic cancer imaging. However, the use of 18F-FDG in PET-based imaging is limited by its specificity and sensitivity. In contrast, anti-TAG (tumor associated glycoprotein)-72 monoclonal antibodies are highly specific for binding to a variety of adenocarcinomas, including colorectal cancer. The aim of this preliminary study was to evaluate a complimentary determining region (CDR)-grafted humanized $ C_{H} $2-domain-deleted anti-TAG-72 monoclonal antibody ($ HuCC49deltaC_{H} $2), radiolabeled with iodine-124 (124I), as an antigen-directed and cancer-specific targeting agent for PET-based imaging. Methods $ HuCC49deltaC_{H} $2 was radiolabeled with 124I. Subcutaneous tumor implants of LS174T colon adenocarcinoma cells, which express TAG-72 antigen, were grown on athymic Nu/Nu nude mice as the xenograft model. Intravascular (i.v.) and intraperitoneal (i.p.) administration of 124I-$ HuCC49deltaC_{H} $2 was then evaluated in this xenograft mouse model at various time points from approximately 1 hour to 24 hours after injection using microPET imaging. This was compared to i.v. injection of 18F-FDG in the same xenograft mouse model using microPET imaging at 50 minutes after injection. Results At approximately 1 hour after i.v. injection, 124I-$ HuCC49deltaC_{H} $2 was distributed within the systemic circulation, while at approximately 1 hour after i.p. injection, 124I-$ HuCC49deltaC_{H} $2 was distributed within the peritoneal cavity. At time points from 18 hours to 24 hours after i.v. and i.p. injection, 124I-$ HuCC49deltaC_{H} $2 demonstrated a significantly increased level of specific localization to LS174T tumor implants (p = 0.001) when compared to the 1 hour images. In contrast, approximately 50 minutes after i.v. injection, 18F-FDG failed to demonstrate any increased level of specific localization to a LS174T tumor implant, but showed the propensity toward more nonspecific uptake within the heart, Harderian glands of the bony orbits of the eyes, brown fat of the posterior neck, kidneys, and bladder. Conclusions On microPET imaging, 124I-$ HuCC49deltaC_{H} $2 demonstrates an increased level of specific localization to tumor implants of LS174T colon adenocarcinoma cells in the xenograft mouse model on delayed imaging, while 18F-FDG failed to demonstrate this. The antigen-directed and cancer-specific 124I-radiolabled anti-TAG-72 monoclonal antibody conjugate, 124I-$ HuCC49deltaC_{H} $2, holds future potential for use in human clinical trials for preoperative, intraoperative, and postoperative PET-based imaging strategies, including fused-modality PET-based imaging platforms. | ||
| 650 | 4 | |a Xenograft Mouse |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Xenograft Mouse Model |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a LS174T Cell |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Human Colon Adenocarcinoma Cell |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a microPET Imaging |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Povoski, Stephen P |4 aut | |
| 700 | 1 | |a Hall, Nathan C |4 aut | |
| 700 | 1 | |a Carlton, Michelle M |4 aut | |
| 700 | 1 | |a Hinkle, George H |4 aut | |
| 700 | 1 | |a Xu, Ronald X |4 aut | |
| 700 | 1 | |a Mojzisik, Cathy M |4 aut | |
| 700 | 1 | |a Johnson, Morgan A |4 aut | |
| 700 | 1 | |a Knopp, Michael V |4 aut | |
| 700 | 1 | |a Martin, Edward W |4 aut | |
| 700 | 1 | |a Sun, Duxin |4 aut | |
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10.1186/1477-7819-8-65 doi (DE-627)SPR028816021 (SPR)1477-7819-8-65-e DE-627 ger DE-627 rakwb eng Zou, Peng verfasserin aut 124I-$ HuCC49deltaC_{H} $2 for TAG-72 antigen-directed positron emission tomography (PET) imaging of LS174T colon adenocarcinoma tumor implants in xenograft mice: preliminary results 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Zou et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( Background 18F-fluorodeoxyglucose positron emission tomography (18F-FDG-PET) is widely used in diagnostic cancer imaging. However, the use of 18F-FDG in PET-based imaging is limited by its specificity and sensitivity. In contrast, anti-TAG (tumor associated glycoprotein)-72 monoclonal antibodies are highly specific for binding to a variety of adenocarcinomas, including colorectal cancer. The aim of this preliminary study was to evaluate a complimentary determining region (CDR)-grafted humanized $ C_{H} $2-domain-deleted anti-TAG-72 monoclonal antibody ($ HuCC49deltaC_{H} $2), radiolabeled with iodine-124 (124I), as an antigen-directed and cancer-specific targeting agent for PET-based imaging. Methods $ HuCC49deltaC_{H} $2 was radiolabeled with 124I. Subcutaneous tumor implants of LS174T colon adenocarcinoma cells, which express TAG-72 antigen, were grown on athymic Nu/Nu nude mice as the xenograft model. Intravascular (i.v.) and intraperitoneal (i.p.) administration of 124I-$ HuCC49deltaC_{H} $2 was then evaluated in this xenograft mouse model at various time points from approximately 1 hour to 24 hours after injection using microPET imaging. This was compared to i.v. injection of 18F-FDG in the same xenograft mouse model using microPET imaging at 50 minutes after injection. Results At approximately 1 hour after i.v. injection, 124I-$ HuCC49deltaC_{H} $2 was distributed within the systemic circulation, while at approximately 1 hour after i.p. injection, 124I-$ HuCC49deltaC_{H} $2 was distributed within the peritoneal cavity. At time points from 18 hours to 24 hours after i.v. and i.p. injection, 124I-$ HuCC49deltaC_{H} $2 demonstrated a significantly increased level of specific localization to LS174T tumor implants (p = 0.001) when compared to the 1 hour images. In contrast, approximately 50 minutes after i.v. injection, 18F-FDG failed to demonstrate any increased level of specific localization to a LS174T tumor implant, but showed the propensity toward more nonspecific uptake within the heart, Harderian glands of the bony orbits of the eyes, brown fat of the posterior neck, kidneys, and bladder. Conclusions On microPET imaging, 124I-$ HuCC49deltaC_{H} $2 demonstrates an increased level of specific localization to tumor implants of LS174T colon adenocarcinoma cells in the xenograft mouse model on delayed imaging, while 18F-FDG failed to demonstrate this. The antigen-directed and cancer-specific 124I-radiolabled anti-TAG-72 monoclonal antibody conjugate, 124I-$ HuCC49deltaC_{H} $2, holds future potential for use in human clinical trials for preoperative, intraoperative, and postoperative PET-based imaging strategies, including fused-modality PET-based imaging platforms. Xenograft Mouse (dpeaa)DE-He213 Xenograft Mouse Model (dpeaa)DE-He213 LS174T Cell (dpeaa)DE-He213 Human Colon Adenocarcinoma Cell (dpeaa)DE-He213 microPET Imaging (dpeaa)DE-He213 Povoski, Stephen P aut Hall, Nathan C aut Carlton, Michelle M aut Hinkle, George H aut Xu, Ronald X aut Mojzisik, Cathy M aut Johnson, Morgan A aut Knopp, Michael V aut Martin, Edward W aut Sun, Duxin aut Enthalten in World journal of surgical oncology London : Biomed Central, 2003 8(2010), 1 vom: 06. Aug. (DE-627)369082907 (DE-600)2118383-1 1477-7819 nnns volume:8 year:2010 number:1 day:06 month:08 https://dx.doi.org/10.1186/1477-7819-8-65 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2010 1 06 08 |
| spelling |
10.1186/1477-7819-8-65 doi (DE-627)SPR028816021 (SPR)1477-7819-8-65-e DE-627 ger DE-627 rakwb eng Zou, Peng verfasserin aut 124I-$ HuCC49deltaC_{H} $2 for TAG-72 antigen-directed positron emission tomography (PET) imaging of LS174T colon adenocarcinoma tumor implants in xenograft mice: preliminary results 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Zou et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( Background 18F-fluorodeoxyglucose positron emission tomography (18F-FDG-PET) is widely used in diagnostic cancer imaging. However, the use of 18F-FDG in PET-based imaging is limited by its specificity and sensitivity. In contrast, anti-TAG (tumor associated glycoprotein)-72 monoclonal antibodies are highly specific for binding to a variety of adenocarcinomas, including colorectal cancer. The aim of this preliminary study was to evaluate a complimentary determining region (CDR)-grafted humanized $ C_{H} $2-domain-deleted anti-TAG-72 monoclonal antibody ($ HuCC49deltaC_{H} $2), radiolabeled with iodine-124 (124I), as an antigen-directed and cancer-specific targeting agent for PET-based imaging. Methods $ HuCC49deltaC_{H} $2 was radiolabeled with 124I. Subcutaneous tumor implants of LS174T colon adenocarcinoma cells, which express TAG-72 antigen, were grown on athymic Nu/Nu nude mice as the xenograft model. Intravascular (i.v.) and intraperitoneal (i.p.) administration of 124I-$ HuCC49deltaC_{H} $2 was then evaluated in this xenograft mouse model at various time points from approximately 1 hour to 24 hours after injection using microPET imaging. This was compared to i.v. injection of 18F-FDG in the same xenograft mouse model using microPET imaging at 50 minutes after injection. Results At approximately 1 hour after i.v. injection, 124I-$ HuCC49deltaC_{H} $2 was distributed within the systemic circulation, while at approximately 1 hour after i.p. injection, 124I-$ HuCC49deltaC_{H} $2 was distributed within the peritoneal cavity. At time points from 18 hours to 24 hours after i.v. and i.p. injection, 124I-$ HuCC49deltaC_{H} $2 demonstrated a significantly increased level of specific localization to LS174T tumor implants (p = 0.001) when compared to the 1 hour images. In contrast, approximately 50 minutes after i.v. injection, 18F-FDG failed to demonstrate any increased level of specific localization to a LS174T tumor implant, but showed the propensity toward more nonspecific uptake within the heart, Harderian glands of the bony orbits of the eyes, brown fat of the posterior neck, kidneys, and bladder. Conclusions On microPET imaging, 124I-$ HuCC49deltaC_{H} $2 demonstrates an increased level of specific localization to tumor implants of LS174T colon adenocarcinoma cells in the xenograft mouse model on delayed imaging, while 18F-FDG failed to demonstrate this. The antigen-directed and cancer-specific 124I-radiolabled anti-TAG-72 monoclonal antibody conjugate, 124I-$ HuCC49deltaC_{H} $2, holds future potential for use in human clinical trials for preoperative, intraoperative, and postoperative PET-based imaging strategies, including fused-modality PET-based imaging platforms. Xenograft Mouse (dpeaa)DE-He213 Xenograft Mouse Model (dpeaa)DE-He213 LS174T Cell (dpeaa)DE-He213 Human Colon Adenocarcinoma Cell (dpeaa)DE-He213 microPET Imaging (dpeaa)DE-He213 Povoski, Stephen P aut Hall, Nathan C aut Carlton, Michelle M aut Hinkle, George H aut Xu, Ronald X aut Mojzisik, Cathy M aut Johnson, Morgan A aut Knopp, Michael V aut Martin, Edward W aut Sun, Duxin aut Enthalten in World journal of surgical oncology London : Biomed Central, 2003 8(2010), 1 vom: 06. Aug. (DE-627)369082907 (DE-600)2118383-1 1477-7819 nnns volume:8 year:2010 number:1 day:06 month:08 https://dx.doi.org/10.1186/1477-7819-8-65 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2010 1 06 08 |
| allfields_unstemmed |
10.1186/1477-7819-8-65 doi (DE-627)SPR028816021 (SPR)1477-7819-8-65-e DE-627 ger DE-627 rakwb eng Zou, Peng verfasserin aut 124I-$ HuCC49deltaC_{H} $2 for TAG-72 antigen-directed positron emission tomography (PET) imaging of LS174T colon adenocarcinoma tumor implants in xenograft mice: preliminary results 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Zou et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( Background 18F-fluorodeoxyglucose positron emission tomography (18F-FDG-PET) is widely used in diagnostic cancer imaging. However, the use of 18F-FDG in PET-based imaging is limited by its specificity and sensitivity. In contrast, anti-TAG (tumor associated glycoprotein)-72 monoclonal antibodies are highly specific for binding to a variety of adenocarcinomas, including colorectal cancer. The aim of this preliminary study was to evaluate a complimentary determining region (CDR)-grafted humanized $ C_{H} $2-domain-deleted anti-TAG-72 monoclonal antibody ($ HuCC49deltaC_{H} $2), radiolabeled with iodine-124 (124I), as an antigen-directed and cancer-specific targeting agent for PET-based imaging. Methods $ HuCC49deltaC_{H} $2 was radiolabeled with 124I. Subcutaneous tumor implants of LS174T colon adenocarcinoma cells, which express TAG-72 antigen, were grown on athymic Nu/Nu nude mice as the xenograft model. Intravascular (i.v.) and intraperitoneal (i.p.) administration of 124I-$ HuCC49deltaC_{H} $2 was then evaluated in this xenograft mouse model at various time points from approximately 1 hour to 24 hours after injection using microPET imaging. This was compared to i.v. injection of 18F-FDG in the same xenograft mouse model using microPET imaging at 50 minutes after injection. Results At approximately 1 hour after i.v. injection, 124I-$ HuCC49deltaC_{H} $2 was distributed within the systemic circulation, while at approximately 1 hour after i.p. injection, 124I-$ HuCC49deltaC_{H} $2 was distributed within the peritoneal cavity. At time points from 18 hours to 24 hours after i.v. and i.p. injection, 124I-$ HuCC49deltaC_{H} $2 demonstrated a significantly increased level of specific localization to LS174T tumor implants (p = 0.001) when compared to the 1 hour images. In contrast, approximately 50 minutes after i.v. injection, 18F-FDG failed to demonstrate any increased level of specific localization to a LS174T tumor implant, but showed the propensity toward more nonspecific uptake within the heart, Harderian glands of the bony orbits of the eyes, brown fat of the posterior neck, kidneys, and bladder. Conclusions On microPET imaging, 124I-$ HuCC49deltaC_{H} $2 demonstrates an increased level of specific localization to tumor implants of LS174T colon adenocarcinoma cells in the xenograft mouse model on delayed imaging, while 18F-FDG failed to demonstrate this. The antigen-directed and cancer-specific 124I-radiolabled anti-TAG-72 monoclonal antibody conjugate, 124I-$ HuCC49deltaC_{H} $2, holds future potential for use in human clinical trials for preoperative, intraoperative, and postoperative PET-based imaging strategies, including fused-modality PET-based imaging platforms. Xenograft Mouse (dpeaa)DE-He213 Xenograft Mouse Model (dpeaa)DE-He213 LS174T Cell (dpeaa)DE-He213 Human Colon Adenocarcinoma Cell (dpeaa)DE-He213 microPET Imaging (dpeaa)DE-He213 Povoski, Stephen P aut Hall, Nathan C aut Carlton, Michelle M aut Hinkle, George H aut Xu, Ronald X aut Mojzisik, Cathy M aut Johnson, Morgan A aut Knopp, Michael V aut Martin, Edward W aut Sun, Duxin aut Enthalten in World journal of surgical oncology London : Biomed Central, 2003 8(2010), 1 vom: 06. Aug. (DE-627)369082907 (DE-600)2118383-1 1477-7819 nnns volume:8 year:2010 number:1 day:06 month:08 https://dx.doi.org/10.1186/1477-7819-8-65 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2010 1 06 08 |
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10.1186/1477-7819-8-65 doi (DE-627)SPR028816021 (SPR)1477-7819-8-65-e DE-627 ger DE-627 rakwb eng Zou, Peng verfasserin aut 124I-$ HuCC49deltaC_{H} $2 for TAG-72 antigen-directed positron emission tomography (PET) imaging of LS174T colon adenocarcinoma tumor implants in xenograft mice: preliminary results 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Zou et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( Background 18F-fluorodeoxyglucose positron emission tomography (18F-FDG-PET) is widely used in diagnostic cancer imaging. However, the use of 18F-FDG in PET-based imaging is limited by its specificity and sensitivity. In contrast, anti-TAG (tumor associated glycoprotein)-72 monoclonal antibodies are highly specific for binding to a variety of adenocarcinomas, including colorectal cancer. The aim of this preliminary study was to evaluate a complimentary determining region (CDR)-grafted humanized $ C_{H} $2-domain-deleted anti-TAG-72 monoclonal antibody ($ HuCC49deltaC_{H} $2), radiolabeled with iodine-124 (124I), as an antigen-directed and cancer-specific targeting agent for PET-based imaging. Methods $ HuCC49deltaC_{H} $2 was radiolabeled with 124I. Subcutaneous tumor implants of LS174T colon adenocarcinoma cells, which express TAG-72 antigen, were grown on athymic Nu/Nu nude mice as the xenograft model. Intravascular (i.v.) and intraperitoneal (i.p.) administration of 124I-$ HuCC49deltaC_{H} $2 was then evaluated in this xenograft mouse model at various time points from approximately 1 hour to 24 hours after injection using microPET imaging. This was compared to i.v. injection of 18F-FDG in the same xenograft mouse model using microPET imaging at 50 minutes after injection. Results At approximately 1 hour after i.v. injection, 124I-$ HuCC49deltaC_{H} $2 was distributed within the systemic circulation, while at approximately 1 hour after i.p. injection, 124I-$ HuCC49deltaC_{H} $2 was distributed within the peritoneal cavity. At time points from 18 hours to 24 hours after i.v. and i.p. injection, 124I-$ HuCC49deltaC_{H} $2 demonstrated a significantly increased level of specific localization to LS174T tumor implants (p = 0.001) when compared to the 1 hour images. In contrast, approximately 50 minutes after i.v. injection, 18F-FDG failed to demonstrate any increased level of specific localization to a LS174T tumor implant, but showed the propensity toward more nonspecific uptake within the heart, Harderian glands of the bony orbits of the eyes, brown fat of the posterior neck, kidneys, and bladder. Conclusions On microPET imaging, 124I-$ HuCC49deltaC_{H} $2 demonstrates an increased level of specific localization to tumor implants of LS174T colon adenocarcinoma cells in the xenograft mouse model on delayed imaging, while 18F-FDG failed to demonstrate this. The antigen-directed and cancer-specific 124I-radiolabled anti-TAG-72 monoclonal antibody conjugate, 124I-$ HuCC49deltaC_{H} $2, holds future potential for use in human clinical trials for preoperative, intraoperative, and postoperative PET-based imaging strategies, including fused-modality PET-based imaging platforms. Xenograft Mouse (dpeaa)DE-He213 Xenograft Mouse Model (dpeaa)DE-He213 LS174T Cell (dpeaa)DE-He213 Human Colon Adenocarcinoma Cell (dpeaa)DE-He213 microPET Imaging (dpeaa)DE-He213 Povoski, Stephen P aut Hall, Nathan C aut Carlton, Michelle M aut Hinkle, George H aut Xu, Ronald X aut Mojzisik, Cathy M aut Johnson, Morgan A aut Knopp, Michael V aut Martin, Edward W aut Sun, Duxin aut Enthalten in World journal of surgical oncology London : Biomed Central, 2003 8(2010), 1 vom: 06. Aug. (DE-627)369082907 (DE-600)2118383-1 1477-7819 nnns volume:8 year:2010 number:1 day:06 month:08 https://dx.doi.org/10.1186/1477-7819-8-65 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2010 1 06 08 |
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10.1186/1477-7819-8-65 doi (DE-627)SPR028816021 (SPR)1477-7819-8-65-e DE-627 ger DE-627 rakwb eng Zou, Peng verfasserin aut 124I-$ HuCC49deltaC_{H} $2 for TAG-72 antigen-directed positron emission tomography (PET) imaging of LS174T colon adenocarcinoma tumor implants in xenograft mice: preliminary results 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Zou et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( Background 18F-fluorodeoxyglucose positron emission tomography (18F-FDG-PET) is widely used in diagnostic cancer imaging. However, the use of 18F-FDG in PET-based imaging is limited by its specificity and sensitivity. In contrast, anti-TAG (tumor associated glycoprotein)-72 monoclonal antibodies are highly specific for binding to a variety of adenocarcinomas, including colorectal cancer. The aim of this preliminary study was to evaluate a complimentary determining region (CDR)-grafted humanized $ C_{H} $2-domain-deleted anti-TAG-72 monoclonal antibody ($ HuCC49deltaC_{H} $2), radiolabeled with iodine-124 (124I), as an antigen-directed and cancer-specific targeting agent for PET-based imaging. Methods $ HuCC49deltaC_{H} $2 was radiolabeled with 124I. Subcutaneous tumor implants of LS174T colon adenocarcinoma cells, which express TAG-72 antigen, were grown on athymic Nu/Nu nude mice as the xenograft model. Intravascular (i.v.) and intraperitoneal (i.p.) administration of 124I-$ HuCC49deltaC_{H} $2 was then evaluated in this xenograft mouse model at various time points from approximately 1 hour to 24 hours after injection using microPET imaging. This was compared to i.v. injection of 18F-FDG in the same xenograft mouse model using microPET imaging at 50 minutes after injection. Results At approximately 1 hour after i.v. injection, 124I-$ HuCC49deltaC_{H} $2 was distributed within the systemic circulation, while at approximately 1 hour after i.p. injection, 124I-$ HuCC49deltaC_{H} $2 was distributed within the peritoneal cavity. At time points from 18 hours to 24 hours after i.v. and i.p. injection, 124I-$ HuCC49deltaC_{H} $2 demonstrated a significantly increased level of specific localization to LS174T tumor implants (p = 0.001) when compared to the 1 hour images. In contrast, approximately 50 minutes after i.v. injection, 18F-FDG failed to demonstrate any increased level of specific localization to a LS174T tumor implant, but showed the propensity toward more nonspecific uptake within the heart, Harderian glands of the bony orbits of the eyes, brown fat of the posterior neck, kidneys, and bladder. Conclusions On microPET imaging, 124I-$ HuCC49deltaC_{H} $2 demonstrates an increased level of specific localization to tumor implants of LS174T colon adenocarcinoma cells in the xenograft mouse model on delayed imaging, while 18F-FDG failed to demonstrate this. The antigen-directed and cancer-specific 124I-radiolabled anti-TAG-72 monoclonal antibody conjugate, 124I-$ HuCC49deltaC_{H} $2, holds future potential for use in human clinical trials for preoperative, intraoperative, and postoperative PET-based imaging strategies, including fused-modality PET-based imaging platforms. Xenograft Mouse (dpeaa)DE-He213 Xenograft Mouse Model (dpeaa)DE-He213 LS174T Cell (dpeaa)DE-He213 Human Colon Adenocarcinoma Cell (dpeaa)DE-He213 microPET Imaging (dpeaa)DE-He213 Povoski, Stephen P aut Hall, Nathan C aut Carlton, Michelle M aut Hinkle, George H aut Xu, Ronald X aut Mojzisik, Cathy M aut Johnson, Morgan A aut Knopp, Michael V aut Martin, Edward W aut Sun, Duxin aut Enthalten in World journal of surgical oncology London : Biomed Central, 2003 8(2010), 1 vom: 06. Aug. (DE-627)369082907 (DE-600)2118383-1 1477-7819 nnns volume:8 year:2010 number:1 day:06 month:08 https://dx.doi.org/10.1186/1477-7819-8-65 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2010 1 06 08 |
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Zou, Peng @@aut@@ Povoski, Stephen P @@aut@@ Hall, Nathan C @@aut@@ Carlton, Michelle M @@aut@@ Hinkle, George H @@aut@@ Xu, Ronald X @@aut@@ Mojzisik, Cathy M @@aut@@ Johnson, Morgan A @@aut@@ Knopp, Michael V @@aut@@ Martin, Edward W @@aut@@ Sun, Duxin @@aut@@ |
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Methods $ HuCC49deltaC_{H} $2 was radiolabeled with 124I. Subcutaneous tumor implants of LS174T colon adenocarcinoma cells, which express TAG-72 antigen, were grown on athymic Nu/Nu nude mice as the xenograft model. Intravascular (i.v.) and intraperitoneal (i.p.) administration of 124I-$ HuCC49deltaC_{H} $2 was then evaluated in this xenograft mouse model at various time points from approximately 1 hour to 24 hours after injection using microPET imaging. This was compared to i.v. injection of 18F-FDG in the same xenograft mouse model using microPET imaging at 50 minutes after injection. Results At approximately 1 hour after i.v. injection, 124I-$ HuCC49deltaC_{H} $2 was distributed within the systemic circulation, while at approximately 1 hour after i.p. injection, 124I-$ HuCC49deltaC_{H} $2 was distributed within the peritoneal cavity. 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Zou, Peng misc Xenograft Mouse misc Xenograft Mouse Model misc LS174T Cell misc Human Colon Adenocarcinoma Cell misc microPET Imaging 124I-$ HuCC49deltaC_{H} $2 for TAG-72 antigen-directed positron emission tomography (PET) imaging of LS174T colon adenocarcinoma tumor implants in xenograft mice: preliminary results |
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124I-$ HuCC49deltaC_{H} $2 for TAG-72 antigen-directed positron emission tomography (PET) imaging of LS174T colon adenocarcinoma tumor implants in xenograft mice: preliminary results Xenograft Mouse (dpeaa)DE-He213 Xenograft Mouse Model (dpeaa)DE-He213 LS174T Cell (dpeaa)DE-He213 Human Colon Adenocarcinoma Cell (dpeaa)DE-He213 microPET Imaging (dpeaa)DE-He213 |
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124I-$ HuCC49deltaC_{H} $2 for TAG-72 antigen-directed positron emission tomography (PET) imaging of LS174T colon adenocarcinoma tumor implants in xenograft mice: preliminary results |
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124I-$ HuCC49deltaC_{H} $2 for TAG-72 antigen-directed positron emission tomography (PET) imaging of LS174T colon adenocarcinoma tumor implants in xenograft mice: preliminary results |
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Zou, Peng Povoski, Stephen P Hall, Nathan C Carlton, Michelle M Hinkle, George H Xu, Ronald X Mojzisik, Cathy M Johnson, Morgan A Knopp, Michael V Martin, Edward W Sun, Duxin |
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124i-$ hucc49deltac_{h} $2 for tag-72 antigen-directed positron emission tomography (pet) imaging of ls174t colon adenocarcinoma tumor implants in xenograft mice: preliminary results |
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124I-$ HuCC49deltaC_{H} $2 for TAG-72 antigen-directed positron emission tomography (PET) imaging of LS174T colon adenocarcinoma tumor implants in xenograft mice: preliminary results |
| abstract |
Background 18F-fluorodeoxyglucose positron emission tomography (18F-FDG-PET) is widely used in diagnostic cancer imaging. However, the use of 18F-FDG in PET-based imaging is limited by its specificity and sensitivity. In contrast, anti-TAG (tumor associated glycoprotein)-72 monoclonal antibodies are highly specific for binding to a variety of adenocarcinomas, including colorectal cancer. The aim of this preliminary study was to evaluate a complimentary determining region (CDR)-grafted humanized $ C_{H} $2-domain-deleted anti-TAG-72 monoclonal antibody ($ HuCC49deltaC_{H} $2), radiolabeled with iodine-124 (124I), as an antigen-directed and cancer-specific targeting agent for PET-based imaging. Methods $ HuCC49deltaC_{H} $2 was radiolabeled with 124I. Subcutaneous tumor implants of LS174T colon adenocarcinoma cells, which express TAG-72 antigen, were grown on athymic Nu/Nu nude mice as the xenograft model. Intravascular (i.v.) and intraperitoneal (i.p.) administration of 124I-$ HuCC49deltaC_{H} $2 was then evaluated in this xenograft mouse model at various time points from approximately 1 hour to 24 hours after injection using microPET imaging. This was compared to i.v. injection of 18F-FDG in the same xenograft mouse model using microPET imaging at 50 minutes after injection. Results At approximately 1 hour after i.v. injection, 124I-$ HuCC49deltaC_{H} $2 was distributed within the systemic circulation, while at approximately 1 hour after i.p. injection, 124I-$ HuCC49deltaC_{H} $2 was distributed within the peritoneal cavity. At time points from 18 hours to 24 hours after i.v. and i.p. injection, 124I-$ HuCC49deltaC_{H} $2 demonstrated a significantly increased level of specific localization to LS174T tumor implants (p = 0.001) when compared to the 1 hour images. In contrast, approximately 50 minutes after i.v. injection, 18F-FDG failed to demonstrate any increased level of specific localization to a LS174T tumor implant, but showed the propensity toward more nonspecific uptake within the heart, Harderian glands of the bony orbits of the eyes, brown fat of the posterior neck, kidneys, and bladder. Conclusions On microPET imaging, 124I-$ HuCC49deltaC_{H} $2 demonstrates an increased level of specific localization to tumor implants of LS174T colon adenocarcinoma cells in the xenograft mouse model on delayed imaging, while 18F-FDG failed to demonstrate this. The antigen-directed and cancer-specific 124I-radiolabled anti-TAG-72 monoclonal antibody conjugate, 124I-$ HuCC49deltaC_{H} $2, holds future potential for use in human clinical trials for preoperative, intraoperative, and postoperative PET-based imaging strategies, including fused-modality PET-based imaging platforms. © Zou et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( |
| abstractGer |
Background 18F-fluorodeoxyglucose positron emission tomography (18F-FDG-PET) is widely used in diagnostic cancer imaging. However, the use of 18F-FDG in PET-based imaging is limited by its specificity and sensitivity. In contrast, anti-TAG (tumor associated glycoprotein)-72 monoclonal antibodies are highly specific for binding to a variety of adenocarcinomas, including colorectal cancer. The aim of this preliminary study was to evaluate a complimentary determining region (CDR)-grafted humanized $ C_{H} $2-domain-deleted anti-TAG-72 monoclonal antibody ($ HuCC49deltaC_{H} $2), radiolabeled with iodine-124 (124I), as an antigen-directed and cancer-specific targeting agent for PET-based imaging. Methods $ HuCC49deltaC_{H} $2 was radiolabeled with 124I. Subcutaneous tumor implants of LS174T colon adenocarcinoma cells, which express TAG-72 antigen, were grown on athymic Nu/Nu nude mice as the xenograft model. Intravascular (i.v.) and intraperitoneal (i.p.) administration of 124I-$ HuCC49deltaC_{H} $2 was then evaluated in this xenograft mouse model at various time points from approximately 1 hour to 24 hours after injection using microPET imaging. This was compared to i.v. injection of 18F-FDG in the same xenograft mouse model using microPET imaging at 50 minutes after injection. Results At approximately 1 hour after i.v. injection, 124I-$ HuCC49deltaC_{H} $2 was distributed within the systemic circulation, while at approximately 1 hour after i.p. injection, 124I-$ HuCC49deltaC_{H} $2 was distributed within the peritoneal cavity. At time points from 18 hours to 24 hours after i.v. and i.p. injection, 124I-$ HuCC49deltaC_{H} $2 demonstrated a significantly increased level of specific localization to LS174T tumor implants (p = 0.001) when compared to the 1 hour images. In contrast, approximately 50 minutes after i.v. injection, 18F-FDG failed to demonstrate any increased level of specific localization to a LS174T tumor implant, but showed the propensity toward more nonspecific uptake within the heart, Harderian glands of the bony orbits of the eyes, brown fat of the posterior neck, kidneys, and bladder. Conclusions On microPET imaging, 124I-$ HuCC49deltaC_{H} $2 demonstrates an increased level of specific localization to tumor implants of LS174T colon adenocarcinoma cells in the xenograft mouse model on delayed imaging, while 18F-FDG failed to demonstrate this. The antigen-directed and cancer-specific 124I-radiolabled anti-TAG-72 monoclonal antibody conjugate, 124I-$ HuCC49deltaC_{H} $2, holds future potential for use in human clinical trials for preoperative, intraoperative, and postoperative PET-based imaging strategies, including fused-modality PET-based imaging platforms. © Zou et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( |
| abstract_unstemmed |
Background 18F-fluorodeoxyglucose positron emission tomography (18F-FDG-PET) is widely used in diagnostic cancer imaging. However, the use of 18F-FDG in PET-based imaging is limited by its specificity and sensitivity. In contrast, anti-TAG (tumor associated glycoprotein)-72 monoclonal antibodies are highly specific for binding to a variety of adenocarcinomas, including colorectal cancer. The aim of this preliminary study was to evaluate a complimentary determining region (CDR)-grafted humanized $ C_{H} $2-domain-deleted anti-TAG-72 monoclonal antibody ($ HuCC49deltaC_{H} $2), radiolabeled with iodine-124 (124I), as an antigen-directed and cancer-specific targeting agent for PET-based imaging. Methods $ HuCC49deltaC_{H} $2 was radiolabeled with 124I. Subcutaneous tumor implants of LS174T colon adenocarcinoma cells, which express TAG-72 antigen, were grown on athymic Nu/Nu nude mice as the xenograft model. Intravascular (i.v.) and intraperitoneal (i.p.) administration of 124I-$ HuCC49deltaC_{H} $2 was then evaluated in this xenograft mouse model at various time points from approximately 1 hour to 24 hours after injection using microPET imaging. This was compared to i.v. injection of 18F-FDG in the same xenograft mouse model using microPET imaging at 50 minutes after injection. Results At approximately 1 hour after i.v. injection, 124I-$ HuCC49deltaC_{H} $2 was distributed within the systemic circulation, while at approximately 1 hour after i.p. injection, 124I-$ HuCC49deltaC_{H} $2 was distributed within the peritoneal cavity. At time points from 18 hours to 24 hours after i.v. and i.p. injection, 124I-$ HuCC49deltaC_{H} $2 demonstrated a significantly increased level of specific localization to LS174T tumor implants (p = 0.001) when compared to the 1 hour images. In contrast, approximately 50 minutes after i.v. injection, 18F-FDG failed to demonstrate any increased level of specific localization to a LS174T tumor implant, but showed the propensity toward more nonspecific uptake within the heart, Harderian glands of the bony orbits of the eyes, brown fat of the posterior neck, kidneys, and bladder. Conclusions On microPET imaging, 124I-$ HuCC49deltaC_{H} $2 demonstrates an increased level of specific localization to tumor implants of LS174T colon adenocarcinoma cells in the xenograft mouse model on delayed imaging, while 18F-FDG failed to demonstrate this. The antigen-directed and cancer-specific 124I-radiolabled anti-TAG-72 monoclonal antibody conjugate, 124I-$ HuCC49deltaC_{H} $2, holds future potential for use in human clinical trials for preoperative, intraoperative, and postoperative PET-based imaging strategies, including fused-modality PET-based imaging platforms. © Zou et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( |
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At time points from 18 hours to 24 hours after i.v. and i.p. injection, 124I-$ HuCC49deltaC_{H} $2 demonstrated a significantly increased level of specific localization to LS174T tumor implants (p = 0.001) when compared to the 1 hour images. In contrast, approximately 50 minutes after i.v. injection, 18F-FDG failed to demonstrate any increased level of specific localization to a LS174T tumor implant, but showed the propensity toward more nonspecific uptake within the heart, Harderian glands of the bony orbits of the eyes, brown fat of the posterior neck, kidneys, and bladder. Conclusions On microPET imaging, 124I-$ HuCC49deltaC_{H} $2 demonstrates an increased level of specific localization to tumor implants of LS174T colon adenocarcinoma cells in the xenograft mouse model on delayed imaging, while 18F-FDG failed to demonstrate this. The antigen-directed and cancer-specific 124I-radiolabled anti-TAG-72 monoclonal antibody conjugate, 124I-$ HuCC49deltaC_{H} $2, holds future potential for use in human clinical trials for preoperative, intraoperative, and postoperative PET-based imaging strategies, including fused-modality PET-based imaging platforms.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Xenograft Mouse</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Xenograft Mouse Model</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">LS174T Cell</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Human Colon Adenocarcinoma Cell</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">microPET Imaging</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Povoski, Stephen P</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Hall, Nathan C</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Carlton, Michelle M</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Hinkle, George H</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Xu, Ronald X</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Mojzisik, Cathy M</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Johnson, Morgan A</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Knopp, Michael V</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Martin, Edward W</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Sun, Duxin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">World journal of surgical oncology</subfield><subfield code="d">London : Biomed Central, 2003</subfield><subfield code="g">8(2010), 1 vom: 06. 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