Expression of HIF-1α and HIF-2α correlates to biological and clinical significance in papillary thyroid carcinoma

Background The aim of this study was to detect the expression of hypoxia-inducible factor (HIF)-1α and HIF-2α in papillary thyroid carcinoma (PTC) compared with normal thyroid tissues. Methods The mRNA levels and protein levels of HIF-1α and HIF-2α were detected by real-time PCR and Western blot sep...
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Autor*in:	Liu, Yan-Mei [verfasserIn] Ying, Shen-Peng Huang, Ying-Rui Pan, Yin Chen, Wei-Jun Ni, Ling-Qin Xu, Jin-Ye Shen, Qin-Yan Liang, Yong

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2016

Schlagwörter:	HIF-1α HIF-2α RT-PCR IHC Western blot

Anmerkung:	© Liu et al. 2016

Übergeordnetes Werk:	Enthalten in: World journal of surgical oncology - London : Biomed Central, 2003, 14(2016), 1 vom: 04. Feb.
Übergeordnetes Werk:	volume:14 ; year:2016 ; number:1 ; day:04 ; month:02

Links:	Volltext

DOI / URN:	10.1186/s12957-016-0785-9

Katalog-ID:	SPR02883366X

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520			\|a Background The aim of this study was to detect the expression of hypoxia-inducible factor (HIF)-1α and HIF-2α in papillary thyroid carcinoma (PTC) compared with normal thyroid tissues. Methods The mRNA levels and protein levels of HIF-1α and HIF-2α were detected by real-time PCR and Western blot separately in 30 pairs of PTCs and normal thyroid cases. The protein levels were also detected by immunohistochemistry (IHC) using 92 samples of PTC group and 46 normal samples as control group for analyzing the biological and clinical significance of the expression of HIF-1α/HIF-2α. Results Real-time PCR results showed the mRNA level of HIF-1α and HIF-2α were significantly higher in PTC than normal group (P < 0.001). Also, significantly higher positive rates (73 %/65 %) of HIF-1α and HIF-2α were observed in PTC compared with the control group (27 %/35 %) by IHC (P < 0.01); the consistent results were gotten with Western blot. Although we did not find a significant correlation between the expression of HIF-1α and HIF-2α with gender, age, calcification, or Hashimoto’s disease in the present study (P > 0.05), both of their expressions were correlated to lymph node metastasis (P < 0.05), capsular invasion (P < 0.05), and TNM stage (P < 0.05). Conclusions Overexpression of HIF-1α and HIF-2α are associated with the carcinogenesis of PTC, served as potential biomarkers of PTC.
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allfields	10.1186/s12957-016-0785-9 doi (DE-627)SPR02883366X (SPR)s12957-016-0785-9-e DE-627 ger DE-627 rakwb eng Liu, Yan-Mei verfasserin aut Expression of HIF-1α and HIF-2α correlates to biological and clinical significance in papillary thyroid carcinoma 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Liu et al. 2016 Background The aim of this study was to detect the expression of hypoxia-inducible factor (HIF)-1α and HIF-2α in papillary thyroid carcinoma (PTC) compared with normal thyroid tissues. Methods The mRNA levels and protein levels of HIF-1α and HIF-2α were detected by real-time PCR and Western blot separately in 30 pairs of PTCs and normal thyroid cases. The protein levels were also detected by immunohistochemistry (IHC) using 92 samples of PTC group and 46 normal samples as control group for analyzing the biological and clinical significance of the expression of HIF-1α/HIF-2α. Results Real-time PCR results showed the mRNA level of HIF-1α and HIF-2α were significantly higher in PTC than normal group (P < 0.001). Also, significantly higher positive rates (73 %/65 %) of HIF-1α and HIF-2α were observed in PTC compared with the control group (27 %/35 %) by IHC (P < 0.01); the consistent results were gotten with Western blot. Although we did not find a significant correlation between the expression of HIF-1α and HIF-2α with gender, age, calcification, or Hashimoto’s disease in the present study (P > 0.05), both of their expressions were correlated to lymph node metastasis (P < 0.05), capsular invasion (P < 0.05), and TNM stage (P < 0.05). Conclusions Overexpression of HIF-1α and HIF-2α are associated with the carcinogenesis of PTC, served as potential biomarkers of PTC. HIF-1α (dpeaa)DE-He213 HIF-2α (dpeaa)DE-He213 RT-PCR (dpeaa)DE-He213 IHC (dpeaa)DE-He213 Western blot (dpeaa)DE-He213 Ying, Shen-Peng aut Huang, Ying-Rui aut Pan, Yin aut Chen, Wei-Jun aut Ni, Ling-Qin aut Xu, Jin-Ye aut Shen, Qin-Yan aut Liang, Yong aut Enthalten in World journal of surgical oncology London : Biomed Central, 2003 14(2016), 1 vom: 04. Feb. (DE-627)369082907 (DE-600)2118383-1 1477-7819 nnns volume:14 year:2016 number:1 day:04 month:02 https://dx.doi.org/10.1186/s12957-016-0785-9 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2016 1 04 02
spelling	10.1186/s12957-016-0785-9 doi (DE-627)SPR02883366X (SPR)s12957-016-0785-9-e DE-627 ger DE-627 rakwb eng Liu, Yan-Mei verfasserin aut Expression of HIF-1α and HIF-2α correlates to biological and clinical significance in papillary thyroid carcinoma 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Liu et al. 2016 Background The aim of this study was to detect the expression of hypoxia-inducible factor (HIF)-1α and HIF-2α in papillary thyroid carcinoma (PTC) compared with normal thyroid tissues. Methods The mRNA levels and protein levels of HIF-1α and HIF-2α were detected by real-time PCR and Western blot separately in 30 pairs of PTCs and normal thyroid cases. The protein levels were also detected by immunohistochemistry (IHC) using 92 samples of PTC group and 46 normal samples as control group for analyzing the biological and clinical significance of the expression of HIF-1α/HIF-2α. Results Real-time PCR results showed the mRNA level of HIF-1α and HIF-2α were significantly higher in PTC than normal group (P < 0.001). Also, significantly higher positive rates (73 %/65 %) of HIF-1α and HIF-2α were observed in PTC compared with the control group (27 %/35 %) by IHC (P < 0.01); the consistent results were gotten with Western blot. Although we did not find a significant correlation between the expression of HIF-1α and HIF-2α with gender, age, calcification, or Hashimoto’s disease in the present study (P > 0.05), both of their expressions were correlated to lymph node metastasis (P < 0.05), capsular invasion (P < 0.05), and TNM stage (P < 0.05). Conclusions Overexpression of HIF-1α and HIF-2α are associated with the carcinogenesis of PTC, served as potential biomarkers of PTC. HIF-1α (dpeaa)DE-He213 HIF-2α (dpeaa)DE-He213 RT-PCR (dpeaa)DE-He213 IHC (dpeaa)DE-He213 Western blot (dpeaa)DE-He213 Ying, Shen-Peng aut Huang, Ying-Rui aut Pan, Yin aut Chen, Wei-Jun aut Ni, Ling-Qin aut Xu, Jin-Ye aut Shen, Qin-Yan aut Liang, Yong aut Enthalten in World journal of surgical oncology London : Biomed Central, 2003 14(2016), 1 vom: 04. Feb. (DE-627)369082907 (DE-600)2118383-1 1477-7819 nnns volume:14 year:2016 number:1 day:04 month:02 https://dx.doi.org/10.1186/s12957-016-0785-9 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2016 1 04 02
allfields_unstemmed	10.1186/s12957-016-0785-9 doi (DE-627)SPR02883366X (SPR)s12957-016-0785-9-e DE-627 ger DE-627 rakwb eng Liu, Yan-Mei verfasserin aut Expression of HIF-1α and HIF-2α correlates to biological and clinical significance in papillary thyroid carcinoma 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Liu et al. 2016 Background The aim of this study was to detect the expression of hypoxia-inducible factor (HIF)-1α and HIF-2α in papillary thyroid carcinoma (PTC) compared with normal thyroid tissues. Methods The mRNA levels and protein levels of HIF-1α and HIF-2α were detected by real-time PCR and Western blot separately in 30 pairs of PTCs and normal thyroid cases. The protein levels were also detected by immunohistochemistry (IHC) using 92 samples of PTC group and 46 normal samples as control group for analyzing the biological and clinical significance of the expression of HIF-1α/HIF-2α. Results Real-time PCR results showed the mRNA level of HIF-1α and HIF-2α were significantly higher in PTC than normal group (P < 0.001). Also, significantly higher positive rates (73 %/65 %) of HIF-1α and HIF-2α were observed in PTC compared with the control group (27 %/35 %) by IHC (P < 0.01); the consistent results were gotten with Western blot. Although we did not find a significant correlation between the expression of HIF-1α and HIF-2α with gender, age, calcification, or Hashimoto’s disease in the present study (P > 0.05), both of their expressions were correlated to lymph node metastasis (P < 0.05), capsular invasion (P < 0.05), and TNM stage (P < 0.05). Conclusions Overexpression of HIF-1α and HIF-2α are associated with the carcinogenesis of PTC, served as potential biomarkers of PTC. HIF-1α (dpeaa)DE-He213 HIF-2α (dpeaa)DE-He213 RT-PCR (dpeaa)DE-He213 IHC (dpeaa)DE-He213 Western blot (dpeaa)DE-He213 Ying, Shen-Peng aut Huang, Ying-Rui aut Pan, Yin aut Chen, Wei-Jun aut Ni, Ling-Qin aut Xu, Jin-Ye aut Shen, Qin-Yan aut Liang, Yong aut Enthalten in World journal of surgical oncology London : Biomed Central, 2003 14(2016), 1 vom: 04. Feb. (DE-627)369082907 (DE-600)2118383-1 1477-7819 nnns volume:14 year:2016 number:1 day:04 month:02 https://dx.doi.org/10.1186/s12957-016-0785-9 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2016 1 04 02
allfieldsGer	10.1186/s12957-016-0785-9 doi (DE-627)SPR02883366X (SPR)s12957-016-0785-9-e DE-627 ger DE-627 rakwb eng Liu, Yan-Mei verfasserin aut Expression of HIF-1α and HIF-2α correlates to biological and clinical significance in papillary thyroid carcinoma 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Liu et al. 2016 Background The aim of this study was to detect the expression of hypoxia-inducible factor (HIF)-1α and HIF-2α in papillary thyroid carcinoma (PTC) compared with normal thyroid tissues. Methods The mRNA levels and protein levels of HIF-1α and HIF-2α were detected by real-time PCR and Western blot separately in 30 pairs of PTCs and normal thyroid cases. The protein levels were also detected by immunohistochemistry (IHC) using 92 samples of PTC group and 46 normal samples as control group for analyzing the biological and clinical significance of the expression of HIF-1α/HIF-2α. Results Real-time PCR results showed the mRNA level of HIF-1α and HIF-2α were significantly higher in PTC than normal group (P < 0.001). Also, significantly higher positive rates (73 %/65 %) of HIF-1α and HIF-2α were observed in PTC compared with the control group (27 %/35 %) by IHC (P < 0.01); the consistent results were gotten with Western blot. Although we did not find a significant correlation between the expression of HIF-1α and HIF-2α with gender, age, calcification, or Hashimoto’s disease in the present study (P > 0.05), both of their expressions were correlated to lymph node metastasis (P < 0.05), capsular invasion (P < 0.05), and TNM stage (P < 0.05). Conclusions Overexpression of HIF-1α and HIF-2α are associated with the carcinogenesis of PTC, served as potential biomarkers of PTC. HIF-1α (dpeaa)DE-He213 HIF-2α (dpeaa)DE-He213 RT-PCR (dpeaa)DE-He213 IHC (dpeaa)DE-He213 Western blot (dpeaa)DE-He213 Ying, Shen-Peng aut Huang, Ying-Rui aut Pan, Yin aut Chen, Wei-Jun aut Ni, Ling-Qin aut Xu, Jin-Ye aut Shen, Qin-Yan aut Liang, Yong aut Enthalten in World journal of surgical oncology London : Biomed Central, 2003 14(2016), 1 vom: 04. Feb. (DE-627)369082907 (DE-600)2118383-1 1477-7819 nnns volume:14 year:2016 number:1 day:04 month:02 https://dx.doi.org/10.1186/s12957-016-0785-9 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2016 1 04 02
allfieldsSound	10.1186/s12957-016-0785-9 doi (DE-627)SPR02883366X (SPR)s12957-016-0785-9-e DE-627 ger DE-627 rakwb eng Liu, Yan-Mei verfasserin aut Expression of HIF-1α and HIF-2α correlates to biological and clinical significance in papillary thyroid carcinoma 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Liu et al. 2016 Background The aim of this study was to detect the expression of hypoxia-inducible factor (HIF)-1α and HIF-2α in papillary thyroid carcinoma (PTC) compared with normal thyroid tissues. Methods The mRNA levels and protein levels of HIF-1α and HIF-2α were detected by real-time PCR and Western blot separately in 30 pairs of PTCs and normal thyroid cases. The protein levels were also detected by immunohistochemistry (IHC) using 92 samples of PTC group and 46 normal samples as control group for analyzing the biological and clinical significance of the expression of HIF-1α/HIF-2α. Results Real-time PCR results showed the mRNA level of HIF-1α and HIF-2α were significantly higher in PTC than normal group (P < 0.001). Also, significantly higher positive rates (73 %/65 %) of HIF-1α and HIF-2α were observed in PTC compared with the control group (27 %/35 %) by IHC (P < 0.01); the consistent results were gotten with Western blot. Although we did not find a significant correlation between the expression of HIF-1α and HIF-2α with gender, age, calcification, or Hashimoto’s disease in the present study (P > 0.05), both of their expressions were correlated to lymph node metastasis (P < 0.05), capsular invasion (P < 0.05), and TNM stage (P < 0.05). Conclusions Overexpression of HIF-1α and HIF-2α are associated with the carcinogenesis of PTC, served as potential biomarkers of PTC. HIF-1α (dpeaa)DE-He213 HIF-2α (dpeaa)DE-He213 RT-PCR (dpeaa)DE-He213 IHC (dpeaa)DE-He213 Western blot (dpeaa)DE-He213 Ying, Shen-Peng aut Huang, Ying-Rui aut Pan, Yin aut Chen, Wei-Jun aut Ni, Ling-Qin aut Xu, Jin-Ye aut Shen, Qin-Yan aut Liang, Yong aut Enthalten in World journal of surgical oncology London : Biomed Central, 2003 14(2016), 1 vom: 04. Feb. (DE-627)369082907 (DE-600)2118383-1 1477-7819 nnns volume:14 year:2016 number:1 day:04 month:02 https://dx.doi.org/10.1186/s12957-016-0785-9 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2016 1 04 02
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source	Enthalten in World journal of surgical oncology 14(2016), 1 vom: 04. Feb. volume:14 year:2016 number:1 day:04 month:02
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Methods The mRNA levels and protein levels of HIF-1α and HIF-2α were detected by real-time PCR and Western blot separately in 30 pairs of PTCs and normal thyroid cases. The protein levels were also detected by immunohistochemistry (IHC) using 92 samples of PTC group and 46 normal samples as control group for analyzing the biological and clinical significance of the expression of HIF-1α/HIF-2α. Results Real-time PCR results showed the mRNA level of HIF-1α and HIF-2α were significantly higher in PTC than normal group (P < 0.001). Also, significantly higher positive rates (73 %/65 %) of HIF-1α and HIF-2α were observed in PTC compared with the control group (27 %/35 %) by IHC (P < 0.01); the consistent results were gotten with Western blot. Although we did not find a significant correlation between the expression of HIF-1α and HIF-2α with gender, age, calcification, or Hashimoto’s disease in the present study (P > 0.05), both of their expressions were correlated to lymph node metastasis (P < 0.05), capsular invasion (P < 0.05), and TNM stage (P < 0.05). 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author	Liu, Yan-Mei
spellingShingle	Liu, Yan-Mei misc HIF-1α misc HIF-2α misc RT-PCR misc IHC misc Western blot Expression of HIF-1α and HIF-2α correlates to biological and clinical significance in papillary thyroid carcinoma
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topic_title	Expression of HIF-1α and HIF-2α correlates to biological and clinical significance in papillary thyroid carcinoma HIF-1α (dpeaa)DE-He213 HIF-2α (dpeaa)DE-He213 RT-PCR (dpeaa)DE-He213 IHC (dpeaa)DE-He213 Western blot (dpeaa)DE-He213
topic	misc HIF-1α misc HIF-2α misc RT-PCR misc IHC misc Western blot
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title	Expression of HIF-1α and HIF-2α correlates to biological and clinical significance in papillary thyroid carcinoma
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title_full	Expression of HIF-1α and HIF-2α correlates to biological and clinical significance in papillary thyroid carcinoma
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author_browse	Liu, Yan-Mei Ying, Shen-Peng Huang, Ying-Rui Pan, Yin Chen, Wei-Jun Ni, Ling-Qin Xu, Jin-Ye Shen, Qin-Yan Liang, Yong
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title_sort	expression of hif-1α and hif-2α correlates to biological and clinical significance in papillary thyroid carcinoma
title_auth	Expression of HIF-1α and HIF-2α correlates to biological and clinical significance in papillary thyroid carcinoma
abstract	Background The aim of this study was to detect the expression of hypoxia-inducible factor (HIF)-1α and HIF-2α in papillary thyroid carcinoma (PTC) compared with normal thyroid tissues. Methods The mRNA levels and protein levels of HIF-1α and HIF-2α were detected by real-time PCR and Western blot separately in 30 pairs of PTCs and normal thyroid cases. The protein levels were also detected by immunohistochemistry (IHC) using 92 samples of PTC group and 46 normal samples as control group for analyzing the biological and clinical significance of the expression of HIF-1α/HIF-2α. Results Real-time PCR results showed the mRNA level of HIF-1α and HIF-2α were significantly higher in PTC than normal group (P < 0.001). Also, significantly higher positive rates (73 %/65 %) of HIF-1α and HIF-2α were observed in PTC compared with the control group (27 %/35 %) by IHC (P < 0.01); the consistent results were gotten with Western blot. Although we did not find a significant correlation between the expression of HIF-1α and HIF-2α with gender, age, calcification, or Hashimoto’s disease in the present study (P > 0.05), both of their expressions were correlated to lymph node metastasis (P < 0.05), capsular invasion (P < 0.05), and TNM stage (P < 0.05). Conclusions Overexpression of HIF-1α and HIF-2α are associated with the carcinogenesis of PTC, served as potential biomarkers of PTC. © Liu et al. 2016
abstractGer	Background The aim of this study was to detect the expression of hypoxia-inducible factor (HIF)-1α and HIF-2α in papillary thyroid carcinoma (PTC) compared with normal thyroid tissues. Methods The mRNA levels and protein levels of HIF-1α and HIF-2α were detected by real-time PCR and Western blot separately in 30 pairs of PTCs and normal thyroid cases. The protein levels were also detected by immunohistochemistry (IHC) using 92 samples of PTC group and 46 normal samples as control group for analyzing the biological and clinical significance of the expression of HIF-1α/HIF-2α. Results Real-time PCR results showed the mRNA level of HIF-1α and HIF-2α were significantly higher in PTC than normal group (P < 0.001). Also, significantly higher positive rates (73 %/65 %) of HIF-1α and HIF-2α were observed in PTC compared with the control group (27 %/35 %) by IHC (P < 0.01); the consistent results were gotten with Western blot. Although we did not find a significant correlation between the expression of HIF-1α and HIF-2α with gender, age, calcification, or Hashimoto’s disease in the present study (P > 0.05), both of their expressions were correlated to lymph node metastasis (P < 0.05), capsular invasion (P < 0.05), and TNM stage (P < 0.05). Conclusions Overexpression of HIF-1α and HIF-2α are associated with the carcinogenesis of PTC, served as potential biomarkers of PTC. © Liu et al. 2016
abstract_unstemmed	Background The aim of this study was to detect the expression of hypoxia-inducible factor (HIF)-1α and HIF-2α in papillary thyroid carcinoma (PTC) compared with normal thyroid tissues. Methods The mRNA levels and protein levels of HIF-1α and HIF-2α were detected by real-time PCR and Western blot separately in 30 pairs of PTCs and normal thyroid cases. The protein levels were also detected by immunohistochemistry (IHC) using 92 samples of PTC group and 46 normal samples as control group for analyzing the biological and clinical significance of the expression of HIF-1α/HIF-2α. Results Real-time PCR results showed the mRNA level of HIF-1α and HIF-2α were significantly higher in PTC than normal group (P < 0.001). Also, significantly higher positive rates (73 %/65 %) of HIF-1α and HIF-2α were observed in PTC compared with the control group (27 %/35 %) by IHC (P < 0.01); the consistent results were gotten with Western blot. Although we did not find a significant correlation between the expression of HIF-1α and HIF-2α with gender, age, calcification, or Hashimoto’s disease in the present study (P > 0.05), both of their expressions were correlated to lymph node metastasis (P < 0.05), capsular invasion (P < 0.05), and TNM stage (P < 0.05). Conclusions Overexpression of HIF-1α and HIF-2α are associated with the carcinogenesis of PTC, served as potential biomarkers of PTC. © Liu et al. 2016
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title_short	Expression of HIF-1α and HIF-2α correlates to biological and clinical significance in papillary thyroid carcinoma
url	https://dx.doi.org/10.1186/s12957-016-0785-9
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author2	Ying, Shen-Peng Huang, Ying-Rui Pan, Yin Chen, Wei-Jun Ni, Ling-Qin Xu, Jin-Ye Shen, Qin-Yan Liang, Yong
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up_date	2024-07-03T22:00:24.755Z
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Methods The mRNA levels and protein levels of HIF-1α and HIF-2α were detected by real-time PCR and Western blot separately in 30 pairs of PTCs and normal thyroid cases. The protein levels were also detected by immunohistochemistry (IHC) using 92 samples of PTC group and 46 normal samples as control group for analyzing the biological and clinical significance of the expression of HIF-1α/HIF-2α. Results Real-time PCR results showed the mRNA level of HIF-1α and HIF-2α were significantly higher in PTC than normal group (P < 0.001). Also, significantly higher positive rates (73 %/65 %) of HIF-1α and HIF-2α were observed in PTC compared with the control group (27 %/35 %) by IHC (P < 0.01); the consistent results were gotten with Western blot. Although we did not find a significant correlation between the expression of HIF-1α and HIF-2α with gender, age, calcification, or Hashimoto’s disease in the present study (P > 0.05), both of their expressions were correlated to lymph node metastasis (P < 0.05), capsular invasion (P < 0.05), and TNM stage (P < 0.05). 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Expression of HIF-1α and HIF-2α correlates to biological and clinical significance in papillary thyroid carcinoma

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