WNT5B governs the phenotype of basal-like breast cancer by activating WNT signaling

Background Breast cancer is the leading cause of cancer-related death in women worldwide. Metastatic disease remains the primary cause of death in patients with breast cancer. Basal-like breast cancer (BLBC) is associated with aggressive behavior, stem-like phenotype, high histological grade, poor c...
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Autor*in:	Jiang, Shaojie [verfasserIn] Zhang, Miaofeng Zhang, Yanhua Zhou, Weiping Zhu, Tao Ruan, Qing Chen, Hui Fang, Jie Zhou, Fei Sun, Jihong Yang, Xiaoming

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2019

Schlagwörter:	WNT5B Basal-like breast cancer Luminal breast cancer Canonical/non-canonical Wnt signaling Epithelial-mesenchymal transition

Anmerkung:	© The Author(s). 2019

Übergeordnetes Werk:	Enthalten in: Cell communication and signaling - London : Biomed Central, 2003, 17(2019), 1 vom: 28. Aug.
Übergeordnetes Werk:	volume:17 ; year:2019 ; number:1 ; day:28 ; month:08

Links:	Volltext

DOI / URN:	10.1186/s12964-019-0419-2

Katalog-ID:	SPR028909011

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520			\|a Background Breast cancer is the leading cause of cancer-related death in women worldwide. Metastatic disease remains the primary cause of death in patients with breast cancer. Basal-like breast cancer (BLBC) is associated with aggressive behavior, stem-like phenotype, high histological grade, poor clinical features, and high rates of recurrences and/or metastasis. However, the mechanism of BLBC phenotype shaping remains obscure. Methods Seventeen normal breast/breast cancer cell lines were used for evaluating the breast cancer subtype-markers, WNT targets and constitutive components, and epithelial mesenchymal transition (EMT) markers analysis by western blot. One hundred and twenty formalin-fixed breast cancer tissues were used for immunohistochemistry (IHC) staining. Nine online platforms (cBioPortal, CCLE, GEPIA, etc.) were used for related analyses. Results We identified Wnt5b as a key regulatory factor that governs the phenotype of BLBC by activating canonical and non-canonical WNT signaling. Wnt5b exhibited basal-like specificity in cells and clinical samples both at the mRNA and protein levels and also showed good correlation with basal-like phenotype at the mRNA level. Besides, Wnt5b was also a promising therapeutic target for LGK-974 treatment. In addition, we identified that CK1α was expressed at low levels in BLBC and that the activation of CK1α by pyrvinium was an alternative strategy for BLBC treatment. Conclusions Wnt5b is not only a diagnostic biomarker but also a potential therapeutic target of BLBC. Graphical abstract
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allfields	10.1186/s12964-019-0419-2 doi (DE-627)SPR028909011 (SPR)s12964-019-0419-2-e DE-627 ger DE-627 rakwb eng Jiang, Shaojie verfasserin aut WNT5B governs the phenotype of basal-like breast cancer by activating WNT signaling 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2019 Background Breast cancer is the leading cause of cancer-related death in women worldwide. Metastatic disease remains the primary cause of death in patients with breast cancer. Basal-like breast cancer (BLBC) is associated with aggressive behavior, stem-like phenotype, high histological grade, poor clinical features, and high rates of recurrences and/or metastasis. However, the mechanism of BLBC phenotype shaping remains obscure. Methods Seventeen normal breast/breast cancer cell lines were used for evaluating the breast cancer subtype-markers, WNT targets and constitutive components, and epithelial mesenchymal transition (EMT) markers analysis by western blot. One hundred and twenty formalin-fixed breast cancer tissues were used for immunohistochemistry (IHC) staining. Nine online platforms (cBioPortal, CCLE, GEPIA, etc.) were used for related analyses. Results We identified Wnt5b as a key regulatory factor that governs the phenotype of BLBC by activating canonical and non-canonical WNT signaling. Wnt5b exhibited basal-like specificity in cells and clinical samples both at the mRNA and protein levels and also showed good correlation with basal-like phenotype at the mRNA level. Besides, Wnt5b was also a promising therapeutic target for LGK-974 treatment. In addition, we identified that CK1α was expressed at low levels in BLBC and that the activation of CK1α by pyrvinium was an alternative strategy for BLBC treatment. Conclusions Wnt5b is not only a diagnostic biomarker but also a potential therapeutic target of BLBC. Graphical abstract WNT5B (dpeaa)DE-He213 Basal-like breast cancer (dpeaa)DE-He213 Luminal breast cancer (dpeaa)DE-He213 Canonical/non-canonical Wnt signaling (dpeaa)DE-He213 Epithelial-mesenchymal transition (dpeaa)DE-He213 Zhang, Miaofeng aut Zhang, Yanhua aut Zhou, Weiping aut Zhu, Tao aut Ruan, Qing aut Chen, Hui aut Fang, Jie aut Zhou, Fei aut Sun, Jihong aut Yang, Xiaoming (orcid)0000-0003-2482-8673 aut Enthalten in Cell communication and signaling London : Biomed Central, 2003 17(2019), 1 vom: 28. Aug. (DE-627)37375275X (DE-600)2126315-2 1478-811X nnns volume:17 year:2019 number:1 day:28 month:08 https://dx.doi.org/10.1186/s12964-019-0419-2 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 17 2019 1 28 08
spelling	10.1186/s12964-019-0419-2 doi (DE-627)SPR028909011 (SPR)s12964-019-0419-2-e DE-627 ger DE-627 rakwb eng Jiang, Shaojie verfasserin aut WNT5B governs the phenotype of basal-like breast cancer by activating WNT signaling 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2019 Background Breast cancer is the leading cause of cancer-related death in women worldwide. Metastatic disease remains the primary cause of death in patients with breast cancer. Basal-like breast cancer (BLBC) is associated with aggressive behavior, stem-like phenotype, high histological grade, poor clinical features, and high rates of recurrences and/or metastasis. However, the mechanism of BLBC phenotype shaping remains obscure. Methods Seventeen normal breast/breast cancer cell lines were used for evaluating the breast cancer subtype-markers, WNT targets and constitutive components, and epithelial mesenchymal transition (EMT) markers analysis by western blot. One hundred and twenty formalin-fixed breast cancer tissues were used for immunohistochemistry (IHC) staining. Nine online platforms (cBioPortal, CCLE, GEPIA, etc.) were used for related analyses. Results We identified Wnt5b as a key regulatory factor that governs the phenotype of BLBC by activating canonical and non-canonical WNT signaling. Wnt5b exhibited basal-like specificity in cells and clinical samples both at the mRNA and protein levels and also showed good correlation with basal-like phenotype at the mRNA level. Besides, Wnt5b was also a promising therapeutic target for LGK-974 treatment. In addition, we identified that CK1α was expressed at low levels in BLBC and that the activation of CK1α by pyrvinium was an alternative strategy for BLBC treatment. Conclusions Wnt5b is not only a diagnostic biomarker but also a potential therapeutic target of BLBC. Graphical abstract WNT5B (dpeaa)DE-He213 Basal-like breast cancer (dpeaa)DE-He213 Luminal breast cancer (dpeaa)DE-He213 Canonical/non-canonical Wnt signaling (dpeaa)DE-He213 Epithelial-mesenchymal transition (dpeaa)DE-He213 Zhang, Miaofeng aut Zhang, Yanhua aut Zhou, Weiping aut Zhu, Tao aut Ruan, Qing aut Chen, Hui aut Fang, Jie aut Zhou, Fei aut Sun, Jihong aut Yang, Xiaoming (orcid)0000-0003-2482-8673 aut Enthalten in Cell communication and signaling London : Biomed Central, 2003 17(2019), 1 vom: 28. Aug. (DE-627)37375275X (DE-600)2126315-2 1478-811X nnns volume:17 year:2019 number:1 day:28 month:08 https://dx.doi.org/10.1186/s12964-019-0419-2 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 17 2019 1 28 08
allfields_unstemmed	10.1186/s12964-019-0419-2 doi (DE-627)SPR028909011 (SPR)s12964-019-0419-2-e DE-627 ger DE-627 rakwb eng Jiang, Shaojie verfasserin aut WNT5B governs the phenotype of basal-like breast cancer by activating WNT signaling 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2019 Background Breast cancer is the leading cause of cancer-related death in women worldwide. Metastatic disease remains the primary cause of death in patients with breast cancer. Basal-like breast cancer (BLBC) is associated with aggressive behavior, stem-like phenotype, high histological grade, poor clinical features, and high rates of recurrences and/or metastasis. However, the mechanism of BLBC phenotype shaping remains obscure. Methods Seventeen normal breast/breast cancer cell lines were used for evaluating the breast cancer subtype-markers, WNT targets and constitutive components, and epithelial mesenchymal transition (EMT) markers analysis by western blot. One hundred and twenty formalin-fixed breast cancer tissues were used for immunohistochemistry (IHC) staining. Nine online platforms (cBioPortal, CCLE, GEPIA, etc.) were used for related analyses. Results We identified Wnt5b as a key regulatory factor that governs the phenotype of BLBC by activating canonical and non-canonical WNT signaling. Wnt5b exhibited basal-like specificity in cells and clinical samples both at the mRNA and protein levels and also showed good correlation with basal-like phenotype at the mRNA level. Besides, Wnt5b was also a promising therapeutic target for LGK-974 treatment. In addition, we identified that CK1α was expressed at low levels in BLBC and that the activation of CK1α by pyrvinium was an alternative strategy for BLBC treatment. Conclusions Wnt5b is not only a diagnostic biomarker but also a potential therapeutic target of BLBC. Graphical abstract WNT5B (dpeaa)DE-He213 Basal-like breast cancer (dpeaa)DE-He213 Luminal breast cancer (dpeaa)DE-He213 Canonical/non-canonical Wnt signaling (dpeaa)DE-He213 Epithelial-mesenchymal transition (dpeaa)DE-He213 Zhang, Miaofeng aut Zhang, Yanhua aut Zhou, Weiping aut Zhu, Tao aut Ruan, Qing aut Chen, Hui aut Fang, Jie aut Zhou, Fei aut Sun, Jihong aut Yang, Xiaoming (orcid)0000-0003-2482-8673 aut Enthalten in Cell communication and signaling London : Biomed Central, 2003 17(2019), 1 vom: 28. Aug. (DE-627)37375275X (DE-600)2126315-2 1478-811X nnns volume:17 year:2019 number:1 day:28 month:08 https://dx.doi.org/10.1186/s12964-019-0419-2 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 17 2019 1 28 08
allfieldsGer	10.1186/s12964-019-0419-2 doi (DE-627)SPR028909011 (SPR)s12964-019-0419-2-e DE-627 ger DE-627 rakwb eng Jiang, Shaojie verfasserin aut WNT5B governs the phenotype of basal-like breast cancer by activating WNT signaling 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2019 Background Breast cancer is the leading cause of cancer-related death in women worldwide. Metastatic disease remains the primary cause of death in patients with breast cancer. Basal-like breast cancer (BLBC) is associated with aggressive behavior, stem-like phenotype, high histological grade, poor clinical features, and high rates of recurrences and/or metastasis. However, the mechanism of BLBC phenotype shaping remains obscure. Methods Seventeen normal breast/breast cancer cell lines were used for evaluating the breast cancer subtype-markers, WNT targets and constitutive components, and epithelial mesenchymal transition (EMT) markers analysis by western blot. One hundred and twenty formalin-fixed breast cancer tissues were used for immunohistochemistry (IHC) staining. Nine online platforms (cBioPortal, CCLE, GEPIA, etc.) were used for related analyses. Results We identified Wnt5b as a key regulatory factor that governs the phenotype of BLBC by activating canonical and non-canonical WNT signaling. Wnt5b exhibited basal-like specificity in cells and clinical samples both at the mRNA and protein levels and also showed good correlation with basal-like phenotype at the mRNA level. Besides, Wnt5b was also a promising therapeutic target for LGK-974 treatment. In addition, we identified that CK1α was expressed at low levels in BLBC and that the activation of CK1α by pyrvinium was an alternative strategy for BLBC treatment. Conclusions Wnt5b is not only a diagnostic biomarker but also a potential therapeutic target of BLBC. Graphical abstract WNT5B (dpeaa)DE-He213 Basal-like breast cancer (dpeaa)DE-He213 Luminal breast cancer (dpeaa)DE-He213 Canonical/non-canonical Wnt signaling (dpeaa)DE-He213 Epithelial-mesenchymal transition (dpeaa)DE-He213 Zhang, Miaofeng aut Zhang, Yanhua aut Zhou, Weiping aut Zhu, Tao aut Ruan, Qing aut Chen, Hui aut Fang, Jie aut Zhou, Fei aut Sun, Jihong aut Yang, Xiaoming (orcid)0000-0003-2482-8673 aut Enthalten in Cell communication and signaling London : Biomed Central, 2003 17(2019), 1 vom: 28. Aug. (DE-627)37375275X (DE-600)2126315-2 1478-811X nnns volume:17 year:2019 number:1 day:28 month:08 https://dx.doi.org/10.1186/s12964-019-0419-2 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 17 2019 1 28 08
allfieldsSound	10.1186/s12964-019-0419-2 doi (DE-627)SPR028909011 (SPR)s12964-019-0419-2-e DE-627 ger DE-627 rakwb eng Jiang, Shaojie verfasserin aut WNT5B governs the phenotype of basal-like breast cancer by activating WNT signaling 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2019 Background Breast cancer is the leading cause of cancer-related death in women worldwide. Metastatic disease remains the primary cause of death in patients with breast cancer. Basal-like breast cancer (BLBC) is associated with aggressive behavior, stem-like phenotype, high histological grade, poor clinical features, and high rates of recurrences and/or metastasis. However, the mechanism of BLBC phenotype shaping remains obscure. Methods Seventeen normal breast/breast cancer cell lines were used for evaluating the breast cancer subtype-markers, WNT targets and constitutive components, and epithelial mesenchymal transition (EMT) markers analysis by western blot. One hundred and twenty formalin-fixed breast cancer tissues were used for immunohistochemistry (IHC) staining. Nine online platforms (cBioPortal, CCLE, GEPIA, etc.) were used for related analyses. Results We identified Wnt5b as a key regulatory factor that governs the phenotype of BLBC by activating canonical and non-canonical WNT signaling. Wnt5b exhibited basal-like specificity in cells and clinical samples both at the mRNA and protein levels and also showed good correlation with basal-like phenotype at the mRNA level. Besides, Wnt5b was also a promising therapeutic target for LGK-974 treatment. In addition, we identified that CK1α was expressed at low levels in BLBC and that the activation of CK1α by pyrvinium was an alternative strategy for BLBC treatment. Conclusions Wnt5b is not only a diagnostic biomarker but also a potential therapeutic target of BLBC. Graphical abstract WNT5B (dpeaa)DE-He213 Basal-like breast cancer (dpeaa)DE-He213 Luminal breast cancer (dpeaa)DE-He213 Canonical/non-canonical Wnt signaling (dpeaa)DE-He213 Epithelial-mesenchymal transition (dpeaa)DE-He213 Zhang, Miaofeng aut Zhang, Yanhua aut Zhou, Weiping aut Zhu, Tao aut Ruan, Qing aut Chen, Hui aut Fang, Jie aut Zhou, Fei aut Sun, Jihong aut Yang, Xiaoming (orcid)0000-0003-2482-8673 aut Enthalten in Cell communication and signaling London : Biomed Central, 2003 17(2019), 1 vom: 28. Aug. (DE-627)37375275X (DE-600)2126315-2 1478-811X nnns volume:17 year:2019 number:1 day:28 month:08 https://dx.doi.org/10.1186/s12964-019-0419-2 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 17 2019 1 28 08
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Metastatic disease remains the primary cause of death in patients with breast cancer. Basal-like breast cancer (BLBC) is associated with aggressive behavior, stem-like phenotype, high histological grade, poor clinical features, and high rates of recurrences and/or metastasis. However, the mechanism of BLBC phenotype shaping remains obscure. Methods Seventeen normal breast/breast cancer cell lines were used for evaluating the breast cancer subtype-markers, WNT targets and constitutive components, and epithelial mesenchymal transition (EMT) markers analysis by western blot. One hundred and twenty formalin-fixed breast cancer tissues were used for immunohistochemistry (IHC) staining. Nine online platforms (cBioPortal, CCLE, GEPIA, etc.) were used for related analyses. Results We identified Wnt5b as a key regulatory factor that governs the phenotype of BLBC by activating canonical and non-canonical WNT signaling. Wnt5b exhibited basal-like specificity in cells and clinical samples both at the mRNA and protein levels and also showed good correlation with basal-like phenotype at the mRNA level. Besides, Wnt5b was also a promising therapeutic target for LGK-974 treatment. In addition, we identified that CK1α was expressed at low levels in BLBC and that the activation of CK1α by pyrvinium was an alternative strategy for BLBC treatment. Conclusions Wnt5b is not only a diagnostic biomarker but also a potential therapeutic target of BLBC. 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author	Jiang, Shaojie
spellingShingle	Jiang, Shaojie misc WNT5B misc Basal-like breast cancer misc Luminal breast cancer misc Canonical/non-canonical Wnt signaling misc Epithelial-mesenchymal transition WNT5B governs the phenotype of basal-like breast cancer by activating WNT signaling
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topic_title	WNT5B governs the phenotype of basal-like breast cancer by activating WNT signaling WNT5B (dpeaa)DE-He213 Basal-like breast cancer (dpeaa)DE-He213 Luminal breast cancer (dpeaa)DE-He213 Canonical/non-canonical Wnt signaling (dpeaa)DE-He213 Epithelial-mesenchymal transition (dpeaa)DE-He213
topic	misc WNT5B misc Basal-like breast cancer misc Luminal breast cancer misc Canonical/non-canonical Wnt signaling misc Epithelial-mesenchymal transition
topic_unstemmed	misc WNT5B misc Basal-like breast cancer misc Luminal breast cancer misc Canonical/non-canonical Wnt signaling misc Epithelial-mesenchymal transition
topic_browse	misc WNT5B misc Basal-like breast cancer misc Luminal breast cancer misc Canonical/non-canonical Wnt signaling misc Epithelial-mesenchymal transition
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title	WNT5B governs the phenotype of basal-like breast cancer by activating WNT signaling
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title_full	WNT5B governs the phenotype of basal-like breast cancer by activating WNT signaling
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title_sort	wnt5b governs the phenotype of basal-like breast cancer by activating wnt signaling
title_auth	WNT5B governs the phenotype of basal-like breast cancer by activating WNT signaling
abstract	Background Breast cancer is the leading cause of cancer-related death in women worldwide. Metastatic disease remains the primary cause of death in patients with breast cancer. Basal-like breast cancer (BLBC) is associated with aggressive behavior, stem-like phenotype, high histological grade, poor clinical features, and high rates of recurrences and/or metastasis. However, the mechanism of BLBC phenotype shaping remains obscure. Methods Seventeen normal breast/breast cancer cell lines were used for evaluating the breast cancer subtype-markers, WNT targets and constitutive components, and epithelial mesenchymal transition (EMT) markers analysis by western blot. One hundred and twenty formalin-fixed breast cancer tissues were used for immunohistochemistry (IHC) staining. Nine online platforms (cBioPortal, CCLE, GEPIA, etc.) were used for related analyses. Results We identified Wnt5b as a key regulatory factor that governs the phenotype of BLBC by activating canonical and non-canonical WNT signaling. Wnt5b exhibited basal-like specificity in cells and clinical samples both at the mRNA and protein levels and also showed good correlation with basal-like phenotype at the mRNA level. Besides, Wnt5b was also a promising therapeutic target for LGK-974 treatment. In addition, we identified that CK1α was expressed at low levels in BLBC and that the activation of CK1α by pyrvinium was an alternative strategy for BLBC treatment. Conclusions Wnt5b is not only a diagnostic biomarker but also a potential therapeutic target of BLBC. Graphical abstract © The Author(s). 2019
abstractGer	Background Breast cancer is the leading cause of cancer-related death in women worldwide. Metastatic disease remains the primary cause of death in patients with breast cancer. Basal-like breast cancer (BLBC) is associated with aggressive behavior, stem-like phenotype, high histological grade, poor clinical features, and high rates of recurrences and/or metastasis. However, the mechanism of BLBC phenotype shaping remains obscure. Methods Seventeen normal breast/breast cancer cell lines were used for evaluating the breast cancer subtype-markers, WNT targets and constitutive components, and epithelial mesenchymal transition (EMT) markers analysis by western blot. One hundred and twenty formalin-fixed breast cancer tissues were used for immunohistochemistry (IHC) staining. Nine online platforms (cBioPortal, CCLE, GEPIA, etc.) were used for related analyses. Results We identified Wnt5b as a key regulatory factor that governs the phenotype of BLBC by activating canonical and non-canonical WNT signaling. Wnt5b exhibited basal-like specificity in cells and clinical samples both at the mRNA and protein levels and also showed good correlation with basal-like phenotype at the mRNA level. Besides, Wnt5b was also a promising therapeutic target for LGK-974 treatment. In addition, we identified that CK1α was expressed at low levels in BLBC and that the activation of CK1α by pyrvinium was an alternative strategy for BLBC treatment. Conclusions Wnt5b is not only a diagnostic biomarker but also a potential therapeutic target of BLBC. Graphical abstract © The Author(s). 2019
abstract_unstemmed	Background Breast cancer is the leading cause of cancer-related death in women worldwide. Metastatic disease remains the primary cause of death in patients with breast cancer. Basal-like breast cancer (BLBC) is associated with aggressive behavior, stem-like phenotype, high histological grade, poor clinical features, and high rates of recurrences and/or metastasis. However, the mechanism of BLBC phenotype shaping remains obscure. Methods Seventeen normal breast/breast cancer cell lines were used for evaluating the breast cancer subtype-markers, WNT targets and constitutive components, and epithelial mesenchymal transition (EMT) markers analysis by western blot. One hundred and twenty formalin-fixed breast cancer tissues were used for immunohistochemistry (IHC) staining. Nine online platforms (cBioPortal, CCLE, GEPIA, etc.) were used for related analyses. Results We identified Wnt5b as a key regulatory factor that governs the phenotype of BLBC by activating canonical and non-canonical WNT signaling. Wnt5b exhibited basal-like specificity in cells and clinical samples both at the mRNA and protein levels and also showed good correlation with basal-like phenotype at the mRNA level. Besides, Wnt5b was also a promising therapeutic target for LGK-974 treatment. In addition, we identified that CK1α was expressed at low levels in BLBC and that the activation of CK1α by pyrvinium was an alternative strategy for BLBC treatment. Conclusions Wnt5b is not only a diagnostic biomarker but also a potential therapeutic target of BLBC. Graphical abstract © The Author(s). 2019
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title_short	WNT5B governs the phenotype of basal-like breast cancer by activating WNT signaling
url	https://dx.doi.org/10.1186/s12964-019-0419-2
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author2	Zhang, Miaofeng Zhang, Yanhua Zhou, Weiping Zhu, Tao Ruan, Qing Chen, Hui Fang, Jie Zhou, Fei Sun, Jihong Yang, Xiaoming
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up_date	2024-07-03T22:26:55.747Z
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Metastatic disease remains the primary cause of death in patients with breast cancer. Basal-like breast cancer (BLBC) is associated with aggressive behavior, stem-like phenotype, high histological grade, poor clinical features, and high rates of recurrences and/or metastasis. However, the mechanism of BLBC phenotype shaping remains obscure. Methods Seventeen normal breast/breast cancer cell lines were used for evaluating the breast cancer subtype-markers, WNT targets and constitutive components, and epithelial mesenchymal transition (EMT) markers analysis by western blot. One hundred and twenty formalin-fixed breast cancer tissues were used for immunohistochemistry (IHC) staining. Nine online platforms (cBioPortal, CCLE, GEPIA, etc.) were used for related analyses. Results We identified Wnt5b as a key regulatory factor that governs the phenotype of BLBC by activating canonical and non-canonical WNT signaling. Wnt5b exhibited basal-like specificity in cells and clinical samples both at the mRNA and protein levels and also showed good correlation with basal-like phenotype at the mRNA level. Besides, Wnt5b was also a promising therapeutic target for LGK-974 treatment. In addition, we identified that CK1α was expressed at low levels in BLBC and that the activation of CK1α by pyrvinium was an alternative strategy for BLBC treatment. Conclusions Wnt5b is not only a diagnostic biomarker but also a potential therapeutic target of BLBC. 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WNT5B governs the phenotype of basal-like breast cancer by activating WNT signaling

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