Epigenetic control of the ubiquitin carboxyl terminal hydrolase 1 in renal cell carcinoma

Background The ubiquitin carboxyl-terminal hydrolase 1 (UCHL1) gene involved in the regulation of cellular ubiquitin levels plays an important role in different cellular processes including cell growth and differentiation. Aberrant expression of UCHL1 has been found in a number of human solid tumors...
Ausführliche Beschreibung

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Autor*in:	Seliger, Barbara [verfasserIn] Handke, Diana Schabel, Elisabeth Bukur, Juergen Lichtenfels, Rudolf Dammann, Reinhard

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2009

Schlagwörter:	Renal Cell Carcinoma Methylation Status Renal Cell Carcinoma Cell Line Primary Renal Cell Carcinoma Combine Bisulfite Restriction Analysis

Anmerkung:	© Seliger et al; licensee BioMed Central Ltd. 2009

Übergeordnetes Werk:	Enthalten in: Journal of translational medicine - London : BioMed Central, 2003, 7(2009), 1 vom: 26. Okt.
Übergeordnetes Werk:	volume:7 ; year:2009 ; number:1 ; day:26 ; month:10

Links:	Volltext

DOI / URN:	10.1186/1479-5876-7-90

Katalog-ID:	SPR028935330

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520			\|a Background The ubiquitin carboxyl-terminal hydrolase 1 (UCHL1) gene involved in the regulation of cellular ubiquitin levels plays an important role in different cellular processes including cell growth and differentiation. Aberrant expression of UCHL1 has been found in a number of human solid tumors including renal cell carcinoma (RCC). In RCC, UCHL1 overexpression is associated with tumor progression and an altered von Hippel Lindau gene expression. Methods To determine the underlying mechanisms for the heterogeneous UCHL1 expression pattern in RCC the UCHL1 promoter DNA methylation status was determined in 17 RCC cell lines as well as in 32 RCC lesions and corresponding tumor adjacent kidney epithelium using combined bisulfite restriction analysis as well as bisulfite DNA sequencing. Results UCHL1 expression was found in all 32 tumor adjacent kidney epithelium samples. However, the lack of or reduced UCHL1 mRNA and/or protein expression was detected in 13/32 RCC biopsies and 7/17 RCC cell lines and due to either a total or partial methylation of the UCHL1 promoter DNA. Upon 2'-deoxy-5-azacytidine treatment an induction of UCHL1 mRNA and protein expression was found in 9/17 RCC cell lines, which was linked to the demethylation degree of the UCHL1 promoter DNA. Conclusion Promoter hypermethylation represents a mechanism for the silencing of the UCHL1 gene expression in RCC and supports the concept of an epigenetic control for the expression of UCHL1 during disease progression.
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allfields	10.1186/1479-5876-7-90 doi (DE-627)SPR028935330 (SPR)1479-5876-7-90-e DE-627 ger DE-627 rakwb eng Seliger, Barbara verfasserin aut Epigenetic control of the ubiquitin carboxyl terminal hydrolase 1 in renal cell carcinoma 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Seliger et al; licensee BioMed Central Ltd. 2009 Background The ubiquitin carboxyl-terminal hydrolase 1 (UCHL1) gene involved in the regulation of cellular ubiquitin levels plays an important role in different cellular processes including cell growth and differentiation. Aberrant expression of UCHL1 has been found in a number of human solid tumors including renal cell carcinoma (RCC). In RCC, UCHL1 overexpression is associated with tumor progression and an altered von Hippel Lindau gene expression. Methods To determine the underlying mechanisms for the heterogeneous UCHL1 expression pattern in RCC the UCHL1 promoter DNA methylation status was determined in 17 RCC cell lines as well as in 32 RCC lesions and corresponding tumor adjacent kidney epithelium using combined bisulfite restriction analysis as well as bisulfite DNA sequencing. Results UCHL1 expression was found in all 32 tumor adjacent kidney epithelium samples. However, the lack of or reduced UCHL1 mRNA and/or protein expression was detected in 13/32 RCC biopsies and 7/17 RCC cell lines and due to either a total or partial methylation of the UCHL1 promoter DNA. Upon 2'-deoxy-5-azacytidine treatment an induction of UCHL1 mRNA and protein expression was found in 9/17 RCC cell lines, which was linked to the demethylation degree of the UCHL1 promoter DNA. Conclusion Promoter hypermethylation represents a mechanism for the silencing of the UCHL1 gene expression in RCC and supports the concept of an epigenetic control for the expression of UCHL1 during disease progression. Renal Cell Carcinoma (dpeaa)DE-He213 Methylation Status (dpeaa)DE-He213 Renal Cell Carcinoma Cell Line (dpeaa)DE-He213 Primary Renal Cell Carcinoma (dpeaa)DE-He213 Combine Bisulfite Restriction Analysis (dpeaa)DE-He213 Handke, Diana aut Schabel, Elisabeth aut Bukur, Juergen aut Lichtenfels, Rudolf aut Dammann, Reinhard aut Enthalten in Journal of translational medicine London : BioMed Central, 2003 7(2009), 1 vom: 26. Okt. (DE-627)369084136 (DE-600)2118570-0 1479-5876 nnns volume:7 year:2009 number:1 day:26 month:10 https://dx.doi.org/10.1186/1479-5876-7-90 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2009 1 26 10
spelling	10.1186/1479-5876-7-90 doi (DE-627)SPR028935330 (SPR)1479-5876-7-90-e DE-627 ger DE-627 rakwb eng Seliger, Barbara verfasserin aut Epigenetic control of the ubiquitin carboxyl terminal hydrolase 1 in renal cell carcinoma 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Seliger et al; licensee BioMed Central Ltd. 2009 Background The ubiquitin carboxyl-terminal hydrolase 1 (UCHL1) gene involved in the regulation of cellular ubiquitin levels plays an important role in different cellular processes including cell growth and differentiation. Aberrant expression of UCHL1 has been found in a number of human solid tumors including renal cell carcinoma (RCC). In RCC, UCHL1 overexpression is associated with tumor progression and an altered von Hippel Lindau gene expression. Methods To determine the underlying mechanisms for the heterogeneous UCHL1 expression pattern in RCC the UCHL1 promoter DNA methylation status was determined in 17 RCC cell lines as well as in 32 RCC lesions and corresponding tumor adjacent kidney epithelium using combined bisulfite restriction analysis as well as bisulfite DNA sequencing. Results UCHL1 expression was found in all 32 tumor adjacent kidney epithelium samples. However, the lack of or reduced UCHL1 mRNA and/or protein expression was detected in 13/32 RCC biopsies and 7/17 RCC cell lines and due to either a total or partial methylation of the UCHL1 promoter DNA. Upon 2'-deoxy-5-azacytidine treatment an induction of UCHL1 mRNA and protein expression was found in 9/17 RCC cell lines, which was linked to the demethylation degree of the UCHL1 promoter DNA. Conclusion Promoter hypermethylation represents a mechanism for the silencing of the UCHL1 gene expression in RCC and supports the concept of an epigenetic control for the expression of UCHL1 during disease progression. Renal Cell Carcinoma (dpeaa)DE-He213 Methylation Status (dpeaa)DE-He213 Renal Cell Carcinoma Cell Line (dpeaa)DE-He213 Primary Renal Cell Carcinoma (dpeaa)DE-He213 Combine Bisulfite Restriction Analysis (dpeaa)DE-He213 Handke, Diana aut Schabel, Elisabeth aut Bukur, Juergen aut Lichtenfels, Rudolf aut Dammann, Reinhard aut Enthalten in Journal of translational medicine London : BioMed Central, 2003 7(2009), 1 vom: 26. Okt. (DE-627)369084136 (DE-600)2118570-0 1479-5876 nnns volume:7 year:2009 number:1 day:26 month:10 https://dx.doi.org/10.1186/1479-5876-7-90 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2009 1 26 10
allfields_unstemmed	10.1186/1479-5876-7-90 doi (DE-627)SPR028935330 (SPR)1479-5876-7-90-e DE-627 ger DE-627 rakwb eng Seliger, Barbara verfasserin aut Epigenetic control of the ubiquitin carboxyl terminal hydrolase 1 in renal cell carcinoma 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Seliger et al; licensee BioMed Central Ltd. 2009 Background The ubiquitin carboxyl-terminal hydrolase 1 (UCHL1) gene involved in the regulation of cellular ubiquitin levels plays an important role in different cellular processes including cell growth and differentiation. Aberrant expression of UCHL1 has been found in a number of human solid tumors including renal cell carcinoma (RCC). In RCC, UCHL1 overexpression is associated with tumor progression and an altered von Hippel Lindau gene expression. Methods To determine the underlying mechanisms for the heterogeneous UCHL1 expression pattern in RCC the UCHL1 promoter DNA methylation status was determined in 17 RCC cell lines as well as in 32 RCC lesions and corresponding tumor adjacent kidney epithelium using combined bisulfite restriction analysis as well as bisulfite DNA sequencing. Results UCHL1 expression was found in all 32 tumor adjacent kidney epithelium samples. However, the lack of or reduced UCHL1 mRNA and/or protein expression was detected in 13/32 RCC biopsies and 7/17 RCC cell lines and due to either a total or partial methylation of the UCHL1 promoter DNA. Upon 2'-deoxy-5-azacytidine treatment an induction of UCHL1 mRNA and protein expression was found in 9/17 RCC cell lines, which was linked to the demethylation degree of the UCHL1 promoter DNA. Conclusion Promoter hypermethylation represents a mechanism for the silencing of the UCHL1 gene expression in RCC and supports the concept of an epigenetic control for the expression of UCHL1 during disease progression. Renal Cell Carcinoma (dpeaa)DE-He213 Methylation Status (dpeaa)DE-He213 Renal Cell Carcinoma Cell Line (dpeaa)DE-He213 Primary Renal Cell Carcinoma (dpeaa)DE-He213 Combine Bisulfite Restriction Analysis (dpeaa)DE-He213 Handke, Diana aut Schabel, Elisabeth aut Bukur, Juergen aut Lichtenfels, Rudolf aut Dammann, Reinhard aut Enthalten in Journal of translational medicine London : BioMed Central, 2003 7(2009), 1 vom: 26. Okt. (DE-627)369084136 (DE-600)2118570-0 1479-5876 nnns volume:7 year:2009 number:1 day:26 month:10 https://dx.doi.org/10.1186/1479-5876-7-90 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2009 1 26 10
allfieldsGer	10.1186/1479-5876-7-90 doi (DE-627)SPR028935330 (SPR)1479-5876-7-90-e DE-627 ger DE-627 rakwb eng Seliger, Barbara verfasserin aut Epigenetic control of the ubiquitin carboxyl terminal hydrolase 1 in renal cell carcinoma 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Seliger et al; licensee BioMed Central Ltd. 2009 Background The ubiquitin carboxyl-terminal hydrolase 1 (UCHL1) gene involved in the regulation of cellular ubiquitin levels plays an important role in different cellular processes including cell growth and differentiation. Aberrant expression of UCHL1 has been found in a number of human solid tumors including renal cell carcinoma (RCC). In RCC, UCHL1 overexpression is associated with tumor progression and an altered von Hippel Lindau gene expression. Methods To determine the underlying mechanisms for the heterogeneous UCHL1 expression pattern in RCC the UCHL1 promoter DNA methylation status was determined in 17 RCC cell lines as well as in 32 RCC lesions and corresponding tumor adjacent kidney epithelium using combined bisulfite restriction analysis as well as bisulfite DNA sequencing. Results UCHL1 expression was found in all 32 tumor adjacent kidney epithelium samples. However, the lack of or reduced UCHL1 mRNA and/or protein expression was detected in 13/32 RCC biopsies and 7/17 RCC cell lines and due to either a total or partial methylation of the UCHL1 promoter DNA. Upon 2'-deoxy-5-azacytidine treatment an induction of UCHL1 mRNA and protein expression was found in 9/17 RCC cell lines, which was linked to the demethylation degree of the UCHL1 promoter DNA. Conclusion Promoter hypermethylation represents a mechanism for the silencing of the UCHL1 gene expression in RCC and supports the concept of an epigenetic control for the expression of UCHL1 during disease progression. Renal Cell Carcinoma (dpeaa)DE-He213 Methylation Status (dpeaa)DE-He213 Renal Cell Carcinoma Cell Line (dpeaa)DE-He213 Primary Renal Cell Carcinoma (dpeaa)DE-He213 Combine Bisulfite Restriction Analysis (dpeaa)DE-He213 Handke, Diana aut Schabel, Elisabeth aut Bukur, Juergen aut Lichtenfels, Rudolf aut Dammann, Reinhard aut Enthalten in Journal of translational medicine London : BioMed Central, 2003 7(2009), 1 vom: 26. Okt. (DE-627)369084136 (DE-600)2118570-0 1479-5876 nnns volume:7 year:2009 number:1 day:26 month:10 https://dx.doi.org/10.1186/1479-5876-7-90 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2009 1 26 10
allfieldsSound	10.1186/1479-5876-7-90 doi (DE-627)SPR028935330 (SPR)1479-5876-7-90-e DE-627 ger DE-627 rakwb eng Seliger, Barbara verfasserin aut Epigenetic control of the ubiquitin carboxyl terminal hydrolase 1 in renal cell carcinoma 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Seliger et al; licensee BioMed Central Ltd. 2009 Background The ubiquitin carboxyl-terminal hydrolase 1 (UCHL1) gene involved in the regulation of cellular ubiquitin levels plays an important role in different cellular processes including cell growth and differentiation. Aberrant expression of UCHL1 has been found in a number of human solid tumors including renal cell carcinoma (RCC). In RCC, UCHL1 overexpression is associated with tumor progression and an altered von Hippel Lindau gene expression. Methods To determine the underlying mechanisms for the heterogeneous UCHL1 expression pattern in RCC the UCHL1 promoter DNA methylation status was determined in 17 RCC cell lines as well as in 32 RCC lesions and corresponding tumor adjacent kidney epithelium using combined bisulfite restriction analysis as well as bisulfite DNA sequencing. Results UCHL1 expression was found in all 32 tumor adjacent kidney epithelium samples. However, the lack of or reduced UCHL1 mRNA and/or protein expression was detected in 13/32 RCC biopsies and 7/17 RCC cell lines and due to either a total or partial methylation of the UCHL1 promoter DNA. Upon 2'-deoxy-5-azacytidine treatment an induction of UCHL1 mRNA and protein expression was found in 9/17 RCC cell lines, which was linked to the demethylation degree of the UCHL1 promoter DNA. Conclusion Promoter hypermethylation represents a mechanism for the silencing of the UCHL1 gene expression in RCC and supports the concept of an epigenetic control for the expression of UCHL1 during disease progression. Renal Cell Carcinoma (dpeaa)DE-He213 Methylation Status (dpeaa)DE-He213 Renal Cell Carcinoma Cell Line (dpeaa)DE-He213 Primary Renal Cell Carcinoma (dpeaa)DE-He213 Combine Bisulfite Restriction Analysis (dpeaa)DE-He213 Handke, Diana aut Schabel, Elisabeth aut Bukur, Juergen aut Lichtenfels, Rudolf aut Dammann, Reinhard aut Enthalten in Journal of translational medicine London : BioMed Central, 2003 7(2009), 1 vom: 26. Okt. (DE-627)369084136 (DE-600)2118570-0 1479-5876 nnns volume:7 year:2009 number:1 day:26 month:10 https://dx.doi.org/10.1186/1479-5876-7-90 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2009 1 26 10
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author	Seliger, Barbara
spellingShingle	Seliger, Barbara misc Renal Cell Carcinoma misc Methylation Status misc Renal Cell Carcinoma Cell Line misc Primary Renal Cell Carcinoma misc Combine Bisulfite Restriction Analysis Epigenetic control of the ubiquitin carboxyl terminal hydrolase 1 in renal cell carcinoma
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topic_title	Epigenetic control of the ubiquitin carboxyl terminal hydrolase 1 in renal cell carcinoma Renal Cell Carcinoma (dpeaa)DE-He213 Methylation Status (dpeaa)DE-He213 Renal Cell Carcinoma Cell Line (dpeaa)DE-He213 Primary Renal Cell Carcinoma (dpeaa)DE-He213 Combine Bisulfite Restriction Analysis (dpeaa)DE-He213
topic	misc Renal Cell Carcinoma misc Methylation Status misc Renal Cell Carcinoma Cell Line misc Primary Renal Cell Carcinoma misc Combine Bisulfite Restriction Analysis
topic_unstemmed	misc Renal Cell Carcinoma misc Methylation Status misc Renal Cell Carcinoma Cell Line misc Primary Renal Cell Carcinoma misc Combine Bisulfite Restriction Analysis
topic_browse	misc Renal Cell Carcinoma misc Methylation Status misc Renal Cell Carcinoma Cell Line misc Primary Renal Cell Carcinoma misc Combine Bisulfite Restriction Analysis
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title	Epigenetic control of the ubiquitin carboxyl terminal hydrolase 1 in renal cell carcinoma
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title_full	Epigenetic control of the ubiquitin carboxyl terminal hydrolase 1 in renal cell carcinoma
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title_sort	epigenetic control of the ubiquitin carboxyl terminal hydrolase 1 in renal cell carcinoma
title_auth	Epigenetic control of the ubiquitin carboxyl terminal hydrolase 1 in renal cell carcinoma
abstract	Background The ubiquitin carboxyl-terminal hydrolase 1 (UCHL1) gene involved in the regulation of cellular ubiquitin levels plays an important role in different cellular processes including cell growth and differentiation. Aberrant expression of UCHL1 has been found in a number of human solid tumors including renal cell carcinoma (RCC). In RCC, UCHL1 overexpression is associated with tumor progression and an altered von Hippel Lindau gene expression. Methods To determine the underlying mechanisms for the heterogeneous UCHL1 expression pattern in RCC the UCHL1 promoter DNA methylation status was determined in 17 RCC cell lines as well as in 32 RCC lesions and corresponding tumor adjacent kidney epithelium using combined bisulfite restriction analysis as well as bisulfite DNA sequencing. Results UCHL1 expression was found in all 32 tumor adjacent kidney epithelium samples. However, the lack of or reduced UCHL1 mRNA and/or protein expression was detected in 13/32 RCC biopsies and 7/17 RCC cell lines and due to either a total or partial methylation of the UCHL1 promoter DNA. Upon 2'-deoxy-5-azacytidine treatment an induction of UCHL1 mRNA and protein expression was found in 9/17 RCC cell lines, which was linked to the demethylation degree of the UCHL1 promoter DNA. Conclusion Promoter hypermethylation represents a mechanism for the silencing of the UCHL1 gene expression in RCC and supports the concept of an epigenetic control for the expression of UCHL1 during disease progression. © Seliger et al; licensee BioMed Central Ltd. 2009
abstractGer	Background The ubiquitin carboxyl-terminal hydrolase 1 (UCHL1) gene involved in the regulation of cellular ubiquitin levels plays an important role in different cellular processes including cell growth and differentiation. Aberrant expression of UCHL1 has been found in a number of human solid tumors including renal cell carcinoma (RCC). In RCC, UCHL1 overexpression is associated with tumor progression and an altered von Hippel Lindau gene expression. Methods To determine the underlying mechanisms for the heterogeneous UCHL1 expression pattern in RCC the UCHL1 promoter DNA methylation status was determined in 17 RCC cell lines as well as in 32 RCC lesions and corresponding tumor adjacent kidney epithelium using combined bisulfite restriction analysis as well as bisulfite DNA sequencing. Results UCHL1 expression was found in all 32 tumor adjacent kidney epithelium samples. However, the lack of or reduced UCHL1 mRNA and/or protein expression was detected in 13/32 RCC biopsies and 7/17 RCC cell lines and due to either a total or partial methylation of the UCHL1 promoter DNA. Upon 2'-deoxy-5-azacytidine treatment an induction of UCHL1 mRNA and protein expression was found in 9/17 RCC cell lines, which was linked to the demethylation degree of the UCHL1 promoter DNA. Conclusion Promoter hypermethylation represents a mechanism for the silencing of the UCHL1 gene expression in RCC and supports the concept of an epigenetic control for the expression of UCHL1 during disease progression. © Seliger et al; licensee BioMed Central Ltd. 2009
abstract_unstemmed	Background The ubiquitin carboxyl-terminal hydrolase 1 (UCHL1) gene involved in the regulation of cellular ubiquitin levels plays an important role in different cellular processes including cell growth and differentiation. Aberrant expression of UCHL1 has been found in a number of human solid tumors including renal cell carcinoma (RCC). In RCC, UCHL1 overexpression is associated with tumor progression and an altered von Hippel Lindau gene expression. Methods To determine the underlying mechanisms for the heterogeneous UCHL1 expression pattern in RCC the UCHL1 promoter DNA methylation status was determined in 17 RCC cell lines as well as in 32 RCC lesions and corresponding tumor adjacent kidney epithelium using combined bisulfite restriction analysis as well as bisulfite DNA sequencing. Results UCHL1 expression was found in all 32 tumor adjacent kidney epithelium samples. However, the lack of or reduced UCHL1 mRNA and/or protein expression was detected in 13/32 RCC biopsies and 7/17 RCC cell lines and due to either a total or partial methylation of the UCHL1 promoter DNA. Upon 2'-deoxy-5-azacytidine treatment an induction of UCHL1 mRNA and protein expression was found in 9/17 RCC cell lines, which was linked to the demethylation degree of the UCHL1 promoter DNA. Conclusion Promoter hypermethylation represents a mechanism for the silencing of the UCHL1 gene expression in RCC and supports the concept of an epigenetic control for the expression of UCHL1 during disease progression. © Seliger et al; licensee BioMed Central Ltd. 2009
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title_short	Epigenetic control of the ubiquitin carboxyl terminal hydrolase 1 in renal cell carcinoma
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Upon 2'-deoxy-5-azacytidine treatment an induction of UCHL1 mRNA and protein expression was found in 9/17 RCC cell lines, which was linked to the demethylation degree of the UCHL1 promoter DNA. 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Epigenetic control of the ubiquitin carboxyl terminal hydrolase 1 in renal cell carcinoma

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