Non-monotonic changes in clonogenic cell survival induced by disulphonated aluminum phthalocyanine photodynamic treatment in a human glioma cell line
Background Photodynamic therapy (PDT) involves excitation of sensitizer molecules by visible light in the presence of molecular oxygen, thereby generating reactive oxygen species (ROS) through electron/energy transfer processes. The ROS, thus produced can cause damage to both the structure and the f...
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| Autor*in: |
Gupta, Seema [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2010 |
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| Anmerkung: |
© Gupta et al; licensee BioMed Central Ltd. 2010 |
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| Übergeordnetes Werk: |
Enthalten in: Journal of translational medicine - London : BioMed Central, 2003, 8(2010), 1 vom: 30. Apr. |
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| Übergeordnetes Werk: |
volume:8 ; year:2010 ; number:1 ; day:30 ; month:04 |
| Links: |
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| DOI / URN: |
10.1186/1479-5876-8-43 |
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| Katalog-ID: |
SPR028936043 |
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| 520 | |a Background Photodynamic therapy (PDT) involves excitation of sensitizer molecules by visible light in the presence of molecular oxygen, thereby generating reactive oxygen species (ROS) through electron/energy transfer processes. The ROS, thus produced can cause damage to both the structure and the function of the cellular constituents resulting in cell death. Our preliminary investigations of dose-response relationships in a human glioma cell line (BMG-1) showed that disulphonated aluminum phthalocyanine ($ AlPcS_{2} $) photodynamically induced loss of cell survival in a concentration dependent manner up to 1 μM, further increases in $ AlPcS_{2} $concentration (>1 μM) were, however, observed to decrease the photodynamic toxicity. Considering the fact that for most photosensitizers only monotonic dose-response (survival) relationships have been reported, this result was unexpected. The present studies were, therefore, undertaken to further investigate the concentration dependent photodynamic effects of $ AlPcS_{2} $. Methods Concentration-dependent cellular uptake, sub-cellular localization, proliferation and photodynamic effects of $ AlPcS_{2} $ were investigated in BMG-1 cells by absorbance and fluorescence measurements, image analysis, cell counting and colony forming assays, flow cytometry and micronuclei formation respectively. Results The cellular uptake as a function of extra-cellular $ AlPcS_{2} $ concentrations was observed to be biphasic. $ AlPcS_{2} $ was distributed throughout the cytoplasm with intense fluorescence in the perinuclear regions at a concentration of 1 μM, while a weak diffuse fluorescence was observed at higher concentrations. A concentration-dependent decrease in cell proliferation with accumulation of cells in $ G_{2} $+M phase was observed after PDT. The response of clonogenic survival after $ AlPcS_{2} $-PDT was non-monotonic with respect to $ AlPcS_{2} $ concentration. Conclusions Based on the results we conclude that concentration-dependent changes in physico-chemical properties of sensitizer such as aggregation may influence intracellular transport and localization of photosensitizer. Consequent modifications in the photodynamic induction of lesions and their repair leading to different modes of cell death may contribute to the observed non-linear effects. | ||
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| 700 | 1 | |a Muralidhar, K |4 aut | |
| 700 | 1 | |a Koru-Sengul, Tulay |4 aut | |
| 700 | 1 | |a Jain, Viney |4 aut | |
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10.1186/1479-5876-8-43 doi (DE-627)SPR028936043 (SPR)1479-5876-8-43-e DE-627 ger DE-627 rakwb eng Gupta, Seema verfasserin aut Non-monotonic changes in clonogenic cell survival induced by disulphonated aluminum phthalocyanine photodynamic treatment in a human glioma cell line 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Gupta et al; licensee BioMed Central Ltd. 2010 Background Photodynamic therapy (PDT) involves excitation of sensitizer molecules by visible light in the presence of molecular oxygen, thereby generating reactive oxygen species (ROS) through electron/energy transfer processes. The ROS, thus produced can cause damage to both the structure and the function of the cellular constituents resulting in cell death. Our preliminary investigations of dose-response relationships in a human glioma cell line (BMG-1) showed that disulphonated aluminum phthalocyanine ($ AlPcS_{2} $) photodynamically induced loss of cell survival in a concentration dependent manner up to 1 μM, further increases in $ AlPcS_{2} $concentration (>1 μM) were, however, observed to decrease the photodynamic toxicity. Considering the fact that for most photosensitizers only monotonic dose-response (survival) relationships have been reported, this result was unexpected. The present studies were, therefore, undertaken to further investigate the concentration dependent photodynamic effects of $ AlPcS_{2} $. Methods Concentration-dependent cellular uptake, sub-cellular localization, proliferation and photodynamic effects of $ AlPcS_{2} $ were investigated in BMG-1 cells by absorbance and fluorescence measurements, image analysis, cell counting and colony forming assays, flow cytometry and micronuclei formation respectively. Results The cellular uptake as a function of extra-cellular $ AlPcS_{2} $ concentrations was observed to be biphasic. $ AlPcS_{2} $ was distributed throughout the cytoplasm with intense fluorescence in the perinuclear regions at a concentration of 1 μM, while a weak diffuse fluorescence was observed at higher concentrations. A concentration-dependent decrease in cell proliferation with accumulation of cells in $ G_{2} $+M phase was observed after PDT. The response of clonogenic survival after $ AlPcS_{2} $-PDT was non-monotonic with respect to $ AlPcS_{2} $ concentration. Conclusions Based on the results we conclude that concentration-dependent changes in physico-chemical properties of sensitizer such as aggregation may influence intracellular transport and localization of photosensitizer. Consequent modifications in the photodynamic induction of lesions and their repair leading to different modes of cell death may contribute to the observed non-linear effects. Phthalocyanine (dpeaa)DE-He213 Clonogenic Survival (dpeaa)DE-He213 Light Dose (dpeaa)DE-He213 Photofrin (dpeaa)DE-He213 Human Glioma Cell Line (dpeaa)DE-He213 Dwarakanath, Bilikere S aut Muralidhar, K aut Koru-Sengul, Tulay aut Jain, Viney aut Enthalten in Journal of translational medicine London : BioMed Central, 2003 8(2010), 1 vom: 30. Apr. (DE-627)369084136 (DE-600)2118570-0 1479-5876 nnns volume:8 year:2010 number:1 day:30 month:04 https://dx.doi.org/10.1186/1479-5876-8-43 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2010 1 30 04 |
| spelling |
10.1186/1479-5876-8-43 doi (DE-627)SPR028936043 (SPR)1479-5876-8-43-e DE-627 ger DE-627 rakwb eng Gupta, Seema verfasserin aut Non-monotonic changes in clonogenic cell survival induced by disulphonated aluminum phthalocyanine photodynamic treatment in a human glioma cell line 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Gupta et al; licensee BioMed Central Ltd. 2010 Background Photodynamic therapy (PDT) involves excitation of sensitizer molecules by visible light in the presence of molecular oxygen, thereby generating reactive oxygen species (ROS) through electron/energy transfer processes. The ROS, thus produced can cause damage to both the structure and the function of the cellular constituents resulting in cell death. Our preliminary investigations of dose-response relationships in a human glioma cell line (BMG-1) showed that disulphonated aluminum phthalocyanine ($ AlPcS_{2} $) photodynamically induced loss of cell survival in a concentration dependent manner up to 1 μM, further increases in $ AlPcS_{2} $concentration (>1 μM) were, however, observed to decrease the photodynamic toxicity. Considering the fact that for most photosensitizers only monotonic dose-response (survival) relationships have been reported, this result was unexpected. The present studies were, therefore, undertaken to further investigate the concentration dependent photodynamic effects of $ AlPcS_{2} $. Methods Concentration-dependent cellular uptake, sub-cellular localization, proliferation and photodynamic effects of $ AlPcS_{2} $ were investigated in BMG-1 cells by absorbance and fluorescence measurements, image analysis, cell counting and colony forming assays, flow cytometry and micronuclei formation respectively. Results The cellular uptake as a function of extra-cellular $ AlPcS_{2} $ concentrations was observed to be biphasic. $ AlPcS_{2} $ was distributed throughout the cytoplasm with intense fluorescence in the perinuclear regions at a concentration of 1 μM, while a weak diffuse fluorescence was observed at higher concentrations. A concentration-dependent decrease in cell proliferation with accumulation of cells in $ G_{2} $+M phase was observed after PDT. The response of clonogenic survival after $ AlPcS_{2} $-PDT was non-monotonic with respect to $ AlPcS_{2} $ concentration. Conclusions Based on the results we conclude that concentration-dependent changes in physico-chemical properties of sensitizer such as aggregation may influence intracellular transport and localization of photosensitizer. Consequent modifications in the photodynamic induction of lesions and their repair leading to different modes of cell death may contribute to the observed non-linear effects. Phthalocyanine (dpeaa)DE-He213 Clonogenic Survival (dpeaa)DE-He213 Light Dose (dpeaa)DE-He213 Photofrin (dpeaa)DE-He213 Human Glioma Cell Line (dpeaa)DE-He213 Dwarakanath, Bilikere S aut Muralidhar, K aut Koru-Sengul, Tulay aut Jain, Viney aut Enthalten in Journal of translational medicine London : BioMed Central, 2003 8(2010), 1 vom: 30. Apr. (DE-627)369084136 (DE-600)2118570-0 1479-5876 nnns volume:8 year:2010 number:1 day:30 month:04 https://dx.doi.org/10.1186/1479-5876-8-43 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2010 1 30 04 |
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10.1186/1479-5876-8-43 doi (DE-627)SPR028936043 (SPR)1479-5876-8-43-e DE-627 ger DE-627 rakwb eng Gupta, Seema verfasserin aut Non-monotonic changes in clonogenic cell survival induced by disulphonated aluminum phthalocyanine photodynamic treatment in a human glioma cell line 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Gupta et al; licensee BioMed Central Ltd. 2010 Background Photodynamic therapy (PDT) involves excitation of sensitizer molecules by visible light in the presence of molecular oxygen, thereby generating reactive oxygen species (ROS) through electron/energy transfer processes. The ROS, thus produced can cause damage to both the structure and the function of the cellular constituents resulting in cell death. Our preliminary investigations of dose-response relationships in a human glioma cell line (BMG-1) showed that disulphonated aluminum phthalocyanine ($ AlPcS_{2} $) photodynamically induced loss of cell survival in a concentration dependent manner up to 1 μM, further increases in $ AlPcS_{2} $concentration (>1 μM) were, however, observed to decrease the photodynamic toxicity. Considering the fact that for most photosensitizers only monotonic dose-response (survival) relationships have been reported, this result was unexpected. The present studies were, therefore, undertaken to further investigate the concentration dependent photodynamic effects of $ AlPcS_{2} $. Methods Concentration-dependent cellular uptake, sub-cellular localization, proliferation and photodynamic effects of $ AlPcS_{2} $ were investigated in BMG-1 cells by absorbance and fluorescence measurements, image analysis, cell counting and colony forming assays, flow cytometry and micronuclei formation respectively. Results The cellular uptake as a function of extra-cellular $ AlPcS_{2} $ concentrations was observed to be biphasic. $ AlPcS_{2} $ was distributed throughout the cytoplasm with intense fluorescence in the perinuclear regions at a concentration of 1 μM, while a weak diffuse fluorescence was observed at higher concentrations. A concentration-dependent decrease in cell proliferation with accumulation of cells in $ G_{2} $+M phase was observed after PDT. The response of clonogenic survival after $ AlPcS_{2} $-PDT was non-monotonic with respect to $ AlPcS_{2} $ concentration. Conclusions Based on the results we conclude that concentration-dependent changes in physico-chemical properties of sensitizer such as aggregation may influence intracellular transport and localization of photosensitizer. Consequent modifications in the photodynamic induction of lesions and their repair leading to different modes of cell death may contribute to the observed non-linear effects. Phthalocyanine (dpeaa)DE-He213 Clonogenic Survival (dpeaa)DE-He213 Light Dose (dpeaa)DE-He213 Photofrin (dpeaa)DE-He213 Human Glioma Cell Line (dpeaa)DE-He213 Dwarakanath, Bilikere S aut Muralidhar, K aut Koru-Sengul, Tulay aut Jain, Viney aut Enthalten in Journal of translational medicine London : BioMed Central, 2003 8(2010), 1 vom: 30. Apr. (DE-627)369084136 (DE-600)2118570-0 1479-5876 nnns volume:8 year:2010 number:1 day:30 month:04 https://dx.doi.org/10.1186/1479-5876-8-43 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2010 1 30 04 |
| allfieldsGer |
10.1186/1479-5876-8-43 doi (DE-627)SPR028936043 (SPR)1479-5876-8-43-e DE-627 ger DE-627 rakwb eng Gupta, Seema verfasserin aut Non-monotonic changes in clonogenic cell survival induced by disulphonated aluminum phthalocyanine photodynamic treatment in a human glioma cell line 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Gupta et al; licensee BioMed Central Ltd. 2010 Background Photodynamic therapy (PDT) involves excitation of sensitizer molecules by visible light in the presence of molecular oxygen, thereby generating reactive oxygen species (ROS) through electron/energy transfer processes. The ROS, thus produced can cause damage to both the structure and the function of the cellular constituents resulting in cell death. Our preliminary investigations of dose-response relationships in a human glioma cell line (BMG-1) showed that disulphonated aluminum phthalocyanine ($ AlPcS_{2} $) photodynamically induced loss of cell survival in a concentration dependent manner up to 1 μM, further increases in $ AlPcS_{2} $concentration (>1 μM) were, however, observed to decrease the photodynamic toxicity. Considering the fact that for most photosensitizers only monotonic dose-response (survival) relationships have been reported, this result was unexpected. The present studies were, therefore, undertaken to further investigate the concentration dependent photodynamic effects of $ AlPcS_{2} $. Methods Concentration-dependent cellular uptake, sub-cellular localization, proliferation and photodynamic effects of $ AlPcS_{2} $ were investigated in BMG-1 cells by absorbance and fluorescence measurements, image analysis, cell counting and colony forming assays, flow cytometry and micronuclei formation respectively. Results The cellular uptake as a function of extra-cellular $ AlPcS_{2} $ concentrations was observed to be biphasic. $ AlPcS_{2} $ was distributed throughout the cytoplasm with intense fluorescence in the perinuclear regions at a concentration of 1 μM, while a weak diffuse fluorescence was observed at higher concentrations. A concentration-dependent decrease in cell proliferation with accumulation of cells in $ G_{2} $+M phase was observed after PDT. The response of clonogenic survival after $ AlPcS_{2} $-PDT was non-monotonic with respect to $ AlPcS_{2} $ concentration. Conclusions Based on the results we conclude that concentration-dependent changes in physico-chemical properties of sensitizer such as aggregation may influence intracellular transport and localization of photosensitizer. Consequent modifications in the photodynamic induction of lesions and their repair leading to different modes of cell death may contribute to the observed non-linear effects. Phthalocyanine (dpeaa)DE-He213 Clonogenic Survival (dpeaa)DE-He213 Light Dose (dpeaa)DE-He213 Photofrin (dpeaa)DE-He213 Human Glioma Cell Line (dpeaa)DE-He213 Dwarakanath, Bilikere S aut Muralidhar, K aut Koru-Sengul, Tulay aut Jain, Viney aut Enthalten in Journal of translational medicine London : BioMed Central, 2003 8(2010), 1 vom: 30. Apr. (DE-627)369084136 (DE-600)2118570-0 1479-5876 nnns volume:8 year:2010 number:1 day:30 month:04 https://dx.doi.org/10.1186/1479-5876-8-43 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2010 1 30 04 |
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10.1186/1479-5876-8-43 doi (DE-627)SPR028936043 (SPR)1479-5876-8-43-e DE-627 ger DE-627 rakwb eng Gupta, Seema verfasserin aut Non-monotonic changes in clonogenic cell survival induced by disulphonated aluminum phthalocyanine photodynamic treatment in a human glioma cell line 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Gupta et al; licensee BioMed Central Ltd. 2010 Background Photodynamic therapy (PDT) involves excitation of sensitizer molecules by visible light in the presence of molecular oxygen, thereby generating reactive oxygen species (ROS) through electron/energy transfer processes. The ROS, thus produced can cause damage to both the structure and the function of the cellular constituents resulting in cell death. Our preliminary investigations of dose-response relationships in a human glioma cell line (BMG-1) showed that disulphonated aluminum phthalocyanine ($ AlPcS_{2} $) photodynamically induced loss of cell survival in a concentration dependent manner up to 1 μM, further increases in $ AlPcS_{2} $concentration (>1 μM) were, however, observed to decrease the photodynamic toxicity. Considering the fact that for most photosensitizers only monotonic dose-response (survival) relationships have been reported, this result was unexpected. The present studies were, therefore, undertaken to further investigate the concentration dependent photodynamic effects of $ AlPcS_{2} $. Methods Concentration-dependent cellular uptake, sub-cellular localization, proliferation and photodynamic effects of $ AlPcS_{2} $ were investigated in BMG-1 cells by absorbance and fluorescence measurements, image analysis, cell counting and colony forming assays, flow cytometry and micronuclei formation respectively. Results The cellular uptake as a function of extra-cellular $ AlPcS_{2} $ concentrations was observed to be biphasic. $ AlPcS_{2} $ was distributed throughout the cytoplasm with intense fluorescence in the perinuclear regions at a concentration of 1 μM, while a weak diffuse fluorescence was observed at higher concentrations. A concentration-dependent decrease in cell proliferation with accumulation of cells in $ G_{2} $+M phase was observed after PDT. The response of clonogenic survival after $ AlPcS_{2} $-PDT was non-monotonic with respect to $ AlPcS_{2} $ concentration. Conclusions Based on the results we conclude that concentration-dependent changes in physico-chemical properties of sensitizer such as aggregation may influence intracellular transport and localization of photosensitizer. Consequent modifications in the photodynamic induction of lesions and their repair leading to different modes of cell death may contribute to the observed non-linear effects. Phthalocyanine (dpeaa)DE-He213 Clonogenic Survival (dpeaa)DE-He213 Light Dose (dpeaa)DE-He213 Photofrin (dpeaa)DE-He213 Human Glioma Cell Line (dpeaa)DE-He213 Dwarakanath, Bilikere S aut Muralidhar, K aut Koru-Sengul, Tulay aut Jain, Viney aut Enthalten in Journal of translational medicine London : BioMed Central, 2003 8(2010), 1 vom: 30. Apr. (DE-627)369084136 (DE-600)2118570-0 1479-5876 nnns volume:8 year:2010 number:1 day:30 month:04 https://dx.doi.org/10.1186/1479-5876-8-43 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2010 1 30 04 |
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Non-monotonic changes in clonogenic cell survival induced by disulphonated aluminum phthalocyanine photodynamic treatment in a human glioma cell line |
| abstract |
Background Photodynamic therapy (PDT) involves excitation of sensitizer molecules by visible light in the presence of molecular oxygen, thereby generating reactive oxygen species (ROS) through electron/energy transfer processes. The ROS, thus produced can cause damage to both the structure and the function of the cellular constituents resulting in cell death. Our preliminary investigations of dose-response relationships in a human glioma cell line (BMG-1) showed that disulphonated aluminum phthalocyanine ($ AlPcS_{2} $) photodynamically induced loss of cell survival in a concentration dependent manner up to 1 μM, further increases in $ AlPcS_{2} $concentration (>1 μM) were, however, observed to decrease the photodynamic toxicity. Considering the fact that for most photosensitizers only monotonic dose-response (survival) relationships have been reported, this result was unexpected. The present studies were, therefore, undertaken to further investigate the concentration dependent photodynamic effects of $ AlPcS_{2} $. Methods Concentration-dependent cellular uptake, sub-cellular localization, proliferation and photodynamic effects of $ AlPcS_{2} $ were investigated in BMG-1 cells by absorbance and fluorescence measurements, image analysis, cell counting and colony forming assays, flow cytometry and micronuclei formation respectively. Results The cellular uptake as a function of extra-cellular $ AlPcS_{2} $ concentrations was observed to be biphasic. $ AlPcS_{2} $ was distributed throughout the cytoplasm with intense fluorescence in the perinuclear regions at a concentration of 1 μM, while a weak diffuse fluorescence was observed at higher concentrations. A concentration-dependent decrease in cell proliferation with accumulation of cells in $ G_{2} $+M phase was observed after PDT. The response of clonogenic survival after $ AlPcS_{2} $-PDT was non-monotonic with respect to $ AlPcS_{2} $ concentration. Conclusions Based on the results we conclude that concentration-dependent changes in physico-chemical properties of sensitizer such as aggregation may influence intracellular transport and localization of photosensitizer. Consequent modifications in the photodynamic induction of lesions and their repair leading to different modes of cell death may contribute to the observed non-linear effects. © Gupta et al; licensee BioMed Central Ltd. 2010 |
| abstractGer |
Background Photodynamic therapy (PDT) involves excitation of sensitizer molecules by visible light in the presence of molecular oxygen, thereby generating reactive oxygen species (ROS) through electron/energy transfer processes. The ROS, thus produced can cause damage to both the structure and the function of the cellular constituents resulting in cell death. Our preliminary investigations of dose-response relationships in a human glioma cell line (BMG-1) showed that disulphonated aluminum phthalocyanine ($ AlPcS_{2} $) photodynamically induced loss of cell survival in a concentration dependent manner up to 1 μM, further increases in $ AlPcS_{2} $concentration (>1 μM) were, however, observed to decrease the photodynamic toxicity. Considering the fact that for most photosensitizers only monotonic dose-response (survival) relationships have been reported, this result was unexpected. The present studies were, therefore, undertaken to further investigate the concentration dependent photodynamic effects of $ AlPcS_{2} $. Methods Concentration-dependent cellular uptake, sub-cellular localization, proliferation and photodynamic effects of $ AlPcS_{2} $ were investigated in BMG-1 cells by absorbance and fluorescence measurements, image analysis, cell counting and colony forming assays, flow cytometry and micronuclei formation respectively. Results The cellular uptake as a function of extra-cellular $ AlPcS_{2} $ concentrations was observed to be biphasic. $ AlPcS_{2} $ was distributed throughout the cytoplasm with intense fluorescence in the perinuclear regions at a concentration of 1 μM, while a weak diffuse fluorescence was observed at higher concentrations. A concentration-dependent decrease in cell proliferation with accumulation of cells in $ G_{2} $+M phase was observed after PDT. The response of clonogenic survival after $ AlPcS_{2} $-PDT was non-monotonic with respect to $ AlPcS_{2} $ concentration. Conclusions Based on the results we conclude that concentration-dependent changes in physico-chemical properties of sensitizer such as aggregation may influence intracellular transport and localization of photosensitizer. Consequent modifications in the photodynamic induction of lesions and their repair leading to different modes of cell death may contribute to the observed non-linear effects. © Gupta et al; licensee BioMed Central Ltd. 2010 |
| abstract_unstemmed |
Background Photodynamic therapy (PDT) involves excitation of sensitizer molecules by visible light in the presence of molecular oxygen, thereby generating reactive oxygen species (ROS) through electron/energy transfer processes. The ROS, thus produced can cause damage to both the structure and the function of the cellular constituents resulting in cell death. Our preliminary investigations of dose-response relationships in a human glioma cell line (BMG-1) showed that disulphonated aluminum phthalocyanine ($ AlPcS_{2} $) photodynamically induced loss of cell survival in a concentration dependent manner up to 1 μM, further increases in $ AlPcS_{2} $concentration (>1 μM) were, however, observed to decrease the photodynamic toxicity. Considering the fact that for most photosensitizers only monotonic dose-response (survival) relationships have been reported, this result was unexpected. The present studies were, therefore, undertaken to further investigate the concentration dependent photodynamic effects of $ AlPcS_{2} $. Methods Concentration-dependent cellular uptake, sub-cellular localization, proliferation and photodynamic effects of $ AlPcS_{2} $ were investigated in BMG-1 cells by absorbance and fluorescence measurements, image analysis, cell counting and colony forming assays, flow cytometry and micronuclei formation respectively. Results The cellular uptake as a function of extra-cellular $ AlPcS_{2} $ concentrations was observed to be biphasic. $ AlPcS_{2} $ was distributed throughout the cytoplasm with intense fluorescence in the perinuclear regions at a concentration of 1 μM, while a weak diffuse fluorescence was observed at higher concentrations. A concentration-dependent decrease in cell proliferation with accumulation of cells in $ G_{2} $+M phase was observed after PDT. The response of clonogenic survival after $ AlPcS_{2} $-PDT was non-monotonic with respect to $ AlPcS_{2} $ concentration. Conclusions Based on the results we conclude that concentration-dependent changes in physico-chemical properties of sensitizer such as aggregation may influence intracellular transport and localization of photosensitizer. Consequent modifications in the photodynamic induction of lesions and their repair leading to different modes of cell death may contribute to the observed non-linear effects. © Gupta et al; licensee BioMed Central Ltd. 2010 |
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Non-monotonic changes in clonogenic cell survival induced by disulphonated aluminum phthalocyanine photodynamic treatment in a human glioma cell line |
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Dwarakanath, Bilikere S Muralidhar, K Koru-Sengul, Tulay Jain, Viney |
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