Diffuse reflectance spectroscopy accurately quantifies various degrees of liver steatosis in murine models of fatty liver disease

Background A real-time objective evaluation for the extent of liver steatosis during liver transplantation is currently not available. Diffuse reflectance spectroscopy (DRS) rapidly and accurately assesses the extent of steatosis in human livers with mild steatosis. However, it is yet unknown whethe...
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Autor*in:	Westerkamp, Andrie C. [verfasserIn] Pully, Vishnu V. Karimian, Golnar Bomfati, Fernanda Veldhuis, Zwanida J. Wiersema-Buist, Janneke Hendriks, Benno H. W. Lisman, Ton Porte, Robert J.

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2015

Schlagwörter:	NASH Diffuse Reflectance Spectroscopy Macrovesicular Steatosis Microvesicular Steatosis Hepatocellular Ballooning

Anmerkung:	© Westerkamp et al. 2015

Übergeordnetes Werk:	Enthalten in: Journal of translational medicine - London : BioMed Central, 2003, 13(2015), 1 vom: 21. Sept.
Übergeordnetes Werk:	volume:13 ; year:2015 ; number:1 ; day:21 ; month:09

Links:	Volltext

DOI / URN:	10.1186/s12967-015-0671-1

Katalog-ID:	SPR028957784

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245	1	0	\|a Diffuse reflectance spectroscopy accurately quantifies various degrees of liver steatosis in murine models of fatty liver disease
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520			\|a Background A real-time objective evaluation for the extent of liver steatosis during liver transplantation is currently not available. Diffuse reflectance spectroscopy (DRS) rapidly and accurately assesses the extent of steatosis in human livers with mild steatosis. However, it is yet unknown whether DRS accurately quantifies moderate/severe steatosis and is able to distinguish between micro- and macrovesicular steatosis. Methods C57BL/6JolaHsd mice were fed wit a choline-deficient l-amino acid-defined diet (CD-AA) or a choline-sufficient l-amino acid-defined control diet (CS-AA) for 3, 8, and 20 weeks. In addition B6.V-Lepob/OlaHsd (ob/ob) mice and their lean controls were studied. A total of 104 DRS measurements were performed in liver tissue ex vivo. The degree of steatosis was quantified from the DRS data and compared with histopathological analysis. Results When assessed by histology, livers of mice fed with a CD-AA and CS-AA diet displayed macrovesicular steatosis (range 0–74 %), ob/ob mice revealed only microvesicular steatosis (range 75–80 %), and their lean controls showed no steatosis. The quantification of steatosis by DRS correlated well with pathology (correlation of 0.76 in CD-AA/CS-AA fed mice and a correlation of 0.75 in ob/ob mice). DRS spectra did not distinguish between micro- and macrovesicular steatosis. In samples from CD-AA/CS-AA fed mice, the DRS was able to distinguish between mild and moderate/severe steatosis with a sensitivity and specificity of 86 and 81 %, respectively. Conclusion DRS can quantify steatosis with good agreement to histopathological analysis. DRS may be useful for real-time objective evaluation of liver steatosis during liver transplantation, especially to differentiate between mild and moderate/severe steatosis.
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700	1		\|a Pully, Vishnu V. \|4 aut
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700	1		\|a Hendriks, Benno H. W. \|4 aut
700	1		\|a Lisman, Ton \|4 aut
700	1		\|a Porte, Robert J. \|4 aut
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allfields	10.1186/s12967-015-0671-1 doi (DE-627)SPR028957784 (SPR)s12967-015-0671-1-e DE-627 ger DE-627 rakwb eng Westerkamp, Andrie C. verfasserin aut Diffuse reflectance spectroscopy accurately quantifies various degrees of liver steatosis in murine models of fatty liver disease 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Westerkamp et al. 2015 Background A real-time objective evaluation for the extent of liver steatosis during liver transplantation is currently not available. Diffuse reflectance spectroscopy (DRS) rapidly and accurately assesses the extent of steatosis in human livers with mild steatosis. However, it is yet unknown whether DRS accurately quantifies moderate/severe steatosis and is able to distinguish between micro- and macrovesicular steatosis. Methods C57BL/6JolaHsd mice were fed wit a choline-deficient l-amino acid-defined diet (CD-AA) or a choline-sufficient l-amino acid-defined control diet (CS-AA) for 3, 8, and 20 weeks. In addition B6.V-Lepob/OlaHsd (ob/ob) mice and their lean controls were studied. A total of 104 DRS measurements were performed in liver tissue ex vivo. The degree of steatosis was quantified from the DRS data and compared with histopathological analysis. Results When assessed by histology, livers of mice fed with a CD-AA and CS-AA diet displayed macrovesicular steatosis (range 0–74 %), ob/ob mice revealed only microvesicular steatosis (range 75–80 %), and their lean controls showed no steatosis. The quantification of steatosis by DRS correlated well with pathology (correlation of 0.76 in CD-AA/CS-AA fed mice and a correlation of 0.75 in ob/ob mice). DRS spectra did not distinguish between micro- and macrovesicular steatosis. In samples from CD-AA/CS-AA fed mice, the DRS was able to distinguish between mild and moderate/severe steatosis with a sensitivity and specificity of 86 and 81 %, respectively. Conclusion DRS can quantify steatosis with good agreement to histopathological analysis. DRS may be useful for real-time objective evaluation of liver steatosis during liver transplantation, especially to differentiate between mild and moderate/severe steatosis. NASH (dpeaa)DE-He213 Diffuse Reflectance Spectroscopy (dpeaa)DE-He213 Macrovesicular Steatosis (dpeaa)DE-He213 Microvesicular Steatosis (dpeaa)DE-He213 Hepatocellular Ballooning (dpeaa)DE-He213 Pully, Vishnu V. aut Karimian, Golnar aut Bomfati, Fernanda aut Veldhuis, Zwanida J. aut Wiersema-Buist, Janneke aut Hendriks, Benno H. W. aut Lisman, Ton aut Porte, Robert J. aut Enthalten in Journal of translational medicine London : BioMed Central, 2003 13(2015), 1 vom: 21. Sept. (DE-627)369084136 (DE-600)2118570-0 1479-5876 nnns volume:13 year:2015 number:1 day:21 month:09 https://dx.doi.org/10.1186/s12967-015-0671-1 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2015 1 21 09
spelling	10.1186/s12967-015-0671-1 doi (DE-627)SPR028957784 (SPR)s12967-015-0671-1-e DE-627 ger DE-627 rakwb eng Westerkamp, Andrie C. verfasserin aut Diffuse reflectance spectroscopy accurately quantifies various degrees of liver steatosis in murine models of fatty liver disease 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Westerkamp et al. 2015 Background A real-time objective evaluation for the extent of liver steatosis during liver transplantation is currently not available. Diffuse reflectance spectroscopy (DRS) rapidly and accurately assesses the extent of steatosis in human livers with mild steatosis. However, it is yet unknown whether DRS accurately quantifies moderate/severe steatosis and is able to distinguish between micro- and macrovesicular steatosis. Methods C57BL/6JolaHsd mice were fed wit a choline-deficient l-amino acid-defined diet (CD-AA) or a choline-sufficient l-amino acid-defined control diet (CS-AA) for 3, 8, and 20 weeks. In addition B6.V-Lepob/OlaHsd (ob/ob) mice and their lean controls were studied. A total of 104 DRS measurements were performed in liver tissue ex vivo. The degree of steatosis was quantified from the DRS data and compared with histopathological analysis. Results When assessed by histology, livers of mice fed with a CD-AA and CS-AA diet displayed macrovesicular steatosis (range 0–74 %), ob/ob mice revealed only microvesicular steatosis (range 75–80 %), and their lean controls showed no steatosis. The quantification of steatosis by DRS correlated well with pathology (correlation of 0.76 in CD-AA/CS-AA fed mice and a correlation of 0.75 in ob/ob mice). DRS spectra did not distinguish between micro- and macrovesicular steatosis. In samples from CD-AA/CS-AA fed mice, the DRS was able to distinguish between mild and moderate/severe steatosis with a sensitivity and specificity of 86 and 81 %, respectively. Conclusion DRS can quantify steatosis with good agreement to histopathological analysis. DRS may be useful for real-time objective evaluation of liver steatosis during liver transplantation, especially to differentiate between mild and moderate/severe steatosis. NASH (dpeaa)DE-He213 Diffuse Reflectance Spectroscopy (dpeaa)DE-He213 Macrovesicular Steatosis (dpeaa)DE-He213 Microvesicular Steatosis (dpeaa)DE-He213 Hepatocellular Ballooning (dpeaa)DE-He213 Pully, Vishnu V. aut Karimian, Golnar aut Bomfati, Fernanda aut Veldhuis, Zwanida J. aut Wiersema-Buist, Janneke aut Hendriks, Benno H. W. aut Lisman, Ton aut Porte, Robert J. aut Enthalten in Journal of translational medicine London : BioMed Central, 2003 13(2015), 1 vom: 21. Sept. (DE-627)369084136 (DE-600)2118570-0 1479-5876 nnns volume:13 year:2015 number:1 day:21 month:09 https://dx.doi.org/10.1186/s12967-015-0671-1 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2015 1 21 09
allfields_unstemmed	10.1186/s12967-015-0671-1 doi (DE-627)SPR028957784 (SPR)s12967-015-0671-1-e DE-627 ger DE-627 rakwb eng Westerkamp, Andrie C. verfasserin aut Diffuse reflectance spectroscopy accurately quantifies various degrees of liver steatosis in murine models of fatty liver disease 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Westerkamp et al. 2015 Background A real-time objective evaluation for the extent of liver steatosis during liver transplantation is currently not available. Diffuse reflectance spectroscopy (DRS) rapidly and accurately assesses the extent of steatosis in human livers with mild steatosis. However, it is yet unknown whether DRS accurately quantifies moderate/severe steatosis and is able to distinguish between micro- and macrovesicular steatosis. Methods C57BL/6JolaHsd mice were fed wit a choline-deficient l-amino acid-defined diet (CD-AA) or a choline-sufficient l-amino acid-defined control diet (CS-AA) for 3, 8, and 20 weeks. In addition B6.V-Lepob/OlaHsd (ob/ob) mice and their lean controls were studied. A total of 104 DRS measurements were performed in liver tissue ex vivo. The degree of steatosis was quantified from the DRS data and compared with histopathological analysis. Results When assessed by histology, livers of mice fed with a CD-AA and CS-AA diet displayed macrovesicular steatosis (range 0–74 %), ob/ob mice revealed only microvesicular steatosis (range 75–80 %), and their lean controls showed no steatosis. The quantification of steatosis by DRS correlated well with pathology (correlation of 0.76 in CD-AA/CS-AA fed mice and a correlation of 0.75 in ob/ob mice). DRS spectra did not distinguish between micro- and macrovesicular steatosis. In samples from CD-AA/CS-AA fed mice, the DRS was able to distinguish between mild and moderate/severe steatosis with a sensitivity and specificity of 86 and 81 %, respectively. Conclusion DRS can quantify steatosis with good agreement to histopathological analysis. DRS may be useful for real-time objective evaluation of liver steatosis during liver transplantation, especially to differentiate between mild and moderate/severe steatosis. NASH (dpeaa)DE-He213 Diffuse Reflectance Spectroscopy (dpeaa)DE-He213 Macrovesicular Steatosis (dpeaa)DE-He213 Microvesicular Steatosis (dpeaa)DE-He213 Hepatocellular Ballooning (dpeaa)DE-He213 Pully, Vishnu V. aut Karimian, Golnar aut Bomfati, Fernanda aut Veldhuis, Zwanida J. aut Wiersema-Buist, Janneke aut Hendriks, Benno H. W. aut Lisman, Ton aut Porte, Robert J. aut Enthalten in Journal of translational medicine London : BioMed Central, 2003 13(2015), 1 vom: 21. Sept. (DE-627)369084136 (DE-600)2118570-0 1479-5876 nnns volume:13 year:2015 number:1 day:21 month:09 https://dx.doi.org/10.1186/s12967-015-0671-1 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2015 1 21 09
allfieldsGer	10.1186/s12967-015-0671-1 doi (DE-627)SPR028957784 (SPR)s12967-015-0671-1-e DE-627 ger DE-627 rakwb eng Westerkamp, Andrie C. verfasserin aut Diffuse reflectance spectroscopy accurately quantifies various degrees of liver steatosis in murine models of fatty liver disease 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Westerkamp et al. 2015 Background A real-time objective evaluation for the extent of liver steatosis during liver transplantation is currently not available. Diffuse reflectance spectroscopy (DRS) rapidly and accurately assesses the extent of steatosis in human livers with mild steatosis. However, it is yet unknown whether DRS accurately quantifies moderate/severe steatosis and is able to distinguish between micro- and macrovesicular steatosis. Methods C57BL/6JolaHsd mice were fed wit a choline-deficient l-amino acid-defined diet (CD-AA) or a choline-sufficient l-amino acid-defined control diet (CS-AA) for 3, 8, and 20 weeks. In addition B6.V-Lepob/OlaHsd (ob/ob) mice and their lean controls were studied. A total of 104 DRS measurements were performed in liver tissue ex vivo. The degree of steatosis was quantified from the DRS data and compared with histopathological analysis. Results When assessed by histology, livers of mice fed with a CD-AA and CS-AA diet displayed macrovesicular steatosis (range 0–74 %), ob/ob mice revealed only microvesicular steatosis (range 75–80 %), and their lean controls showed no steatosis. The quantification of steatosis by DRS correlated well with pathology (correlation of 0.76 in CD-AA/CS-AA fed mice and a correlation of 0.75 in ob/ob mice). DRS spectra did not distinguish between micro- and macrovesicular steatosis. In samples from CD-AA/CS-AA fed mice, the DRS was able to distinguish between mild and moderate/severe steatosis with a sensitivity and specificity of 86 and 81 %, respectively. Conclusion DRS can quantify steatosis with good agreement to histopathological analysis. DRS may be useful for real-time objective evaluation of liver steatosis during liver transplantation, especially to differentiate between mild and moderate/severe steatosis. NASH (dpeaa)DE-He213 Diffuse Reflectance Spectroscopy (dpeaa)DE-He213 Macrovesicular Steatosis (dpeaa)DE-He213 Microvesicular Steatosis (dpeaa)DE-He213 Hepatocellular Ballooning (dpeaa)DE-He213 Pully, Vishnu V. aut Karimian, Golnar aut Bomfati, Fernanda aut Veldhuis, Zwanida J. aut Wiersema-Buist, Janneke aut Hendriks, Benno H. W. aut Lisman, Ton aut Porte, Robert J. aut Enthalten in Journal of translational medicine London : BioMed Central, 2003 13(2015), 1 vom: 21. Sept. (DE-627)369084136 (DE-600)2118570-0 1479-5876 nnns volume:13 year:2015 number:1 day:21 month:09 https://dx.doi.org/10.1186/s12967-015-0671-1 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2015 1 21 09
allfieldsSound	10.1186/s12967-015-0671-1 doi (DE-627)SPR028957784 (SPR)s12967-015-0671-1-e DE-627 ger DE-627 rakwb eng Westerkamp, Andrie C. verfasserin aut Diffuse reflectance spectroscopy accurately quantifies various degrees of liver steatosis in murine models of fatty liver disease 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Westerkamp et al. 2015 Background A real-time objective evaluation for the extent of liver steatosis during liver transplantation is currently not available. Diffuse reflectance spectroscopy (DRS) rapidly and accurately assesses the extent of steatosis in human livers with mild steatosis. However, it is yet unknown whether DRS accurately quantifies moderate/severe steatosis and is able to distinguish between micro- and macrovesicular steatosis. Methods C57BL/6JolaHsd mice were fed wit a choline-deficient l-amino acid-defined diet (CD-AA) or a choline-sufficient l-amino acid-defined control diet (CS-AA) for 3, 8, and 20 weeks. In addition B6.V-Lepob/OlaHsd (ob/ob) mice and their lean controls were studied. A total of 104 DRS measurements were performed in liver tissue ex vivo. The degree of steatosis was quantified from the DRS data and compared with histopathological analysis. Results When assessed by histology, livers of mice fed with a CD-AA and CS-AA diet displayed macrovesicular steatosis (range 0–74 %), ob/ob mice revealed only microvesicular steatosis (range 75–80 %), and their lean controls showed no steatosis. The quantification of steatosis by DRS correlated well with pathology (correlation of 0.76 in CD-AA/CS-AA fed mice and a correlation of 0.75 in ob/ob mice). DRS spectra did not distinguish between micro- and macrovesicular steatosis. In samples from CD-AA/CS-AA fed mice, the DRS was able to distinguish between mild and moderate/severe steatosis with a sensitivity and specificity of 86 and 81 %, respectively. Conclusion DRS can quantify steatosis with good agreement to histopathological analysis. DRS may be useful for real-time objective evaluation of liver steatosis during liver transplantation, especially to differentiate between mild and moderate/severe steatosis. NASH (dpeaa)DE-He213 Diffuse Reflectance Spectroscopy (dpeaa)DE-He213 Macrovesicular Steatosis (dpeaa)DE-He213 Microvesicular Steatosis (dpeaa)DE-He213 Hepatocellular Ballooning (dpeaa)DE-He213 Pully, Vishnu V. aut Karimian, Golnar aut Bomfati, Fernanda aut Veldhuis, Zwanida J. aut Wiersema-Buist, Janneke aut Hendriks, Benno H. W. aut Lisman, Ton aut Porte, Robert J. aut Enthalten in Journal of translational medicine London : BioMed Central, 2003 13(2015), 1 vom: 21. Sept. (DE-627)369084136 (DE-600)2118570-0 1479-5876 nnns volume:13 year:2015 number:1 day:21 month:09 https://dx.doi.org/10.1186/s12967-015-0671-1 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2015 1 21 09
language	English
source	Enthalten in Journal of translational medicine 13(2015), 1 vom: 21. Sept. volume:13 year:2015 number:1 day:21 month:09
sourceStr	Enthalten in Journal of translational medicine 13(2015), 1 vom: 21. Sept. volume:13 year:2015 number:1 day:21 month:09
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	NASH Diffuse Reflectance Spectroscopy Macrovesicular Steatosis Microvesicular Steatosis Hepatocellular Ballooning
isfreeaccess_bool	true
container_title	Journal of translational medicine
authorswithroles_txt_mv	Westerkamp, Andrie C. @@aut@@ Pully, Vishnu V. @@aut@@ Karimian, Golnar @@aut@@ Bomfati, Fernanda @@aut@@ Veldhuis, Zwanida J. @@aut@@ Wiersema-Buist, Janneke @@aut@@ Hendriks, Benno H. W. @@aut@@ Lisman, Ton @@aut@@ Porte, Robert J. @@aut@@
publishDateDaySort_date	2015-09-21T00:00:00Z
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language_de	englisch
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Diffuse reflectance spectroscopy (DRS) rapidly and accurately assesses the extent of steatosis in human livers with mild steatosis. However, it is yet unknown whether DRS accurately quantifies moderate/severe steatosis and is able to distinguish between micro- and macrovesicular steatosis. Methods C57BL/6JolaHsd mice were fed wit a choline-deficient l-amino acid-defined diet (CD-AA) or a choline-sufficient l-amino acid-defined control diet (CS-AA) for 3, 8, and 20 weeks. In addition B6.V-Lepob/OlaHsd (ob/ob) mice and their lean controls were studied. A total of 104 DRS measurements were performed in liver tissue ex vivo. The degree of steatosis was quantified from the DRS data and compared with histopathological analysis. Results When assessed by histology, livers of mice fed with a CD-AA and CS-AA diet displayed macrovesicular steatosis (range 0–74 %), ob/ob mice revealed only microvesicular steatosis (range 75–80 %), and their lean controls showed no steatosis. The quantification of steatosis by DRS correlated well with pathology (correlation of 0.76 in CD-AA/CS-AA fed mice and a correlation of 0.75 in ob/ob mice). DRS spectra did not distinguish between micro- and macrovesicular steatosis. In samples from CD-AA/CS-AA fed mice, the DRS was able to distinguish between mild and moderate/severe steatosis with a sensitivity and specificity of 86 and 81 %, respectively. Conclusion DRS can quantify steatosis with good agreement to histopathological analysis. 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author	Westerkamp, Andrie C.
spellingShingle	Westerkamp, Andrie C. misc NASH misc Diffuse Reflectance Spectroscopy misc Macrovesicular Steatosis misc Microvesicular Steatosis misc Hepatocellular Ballooning Diffuse reflectance spectroscopy accurately quantifies various degrees of liver steatosis in murine models of fatty liver disease
authorStr	Westerkamp, Andrie C.
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topic_title	Diffuse reflectance spectroscopy accurately quantifies various degrees of liver steatosis in murine models of fatty liver disease NASH (dpeaa)DE-He213 Diffuse Reflectance Spectroscopy (dpeaa)DE-He213 Macrovesicular Steatosis (dpeaa)DE-He213 Microvesicular Steatosis (dpeaa)DE-He213 Hepatocellular Ballooning (dpeaa)DE-He213
topic	misc NASH misc Diffuse Reflectance Spectroscopy misc Macrovesicular Steatosis misc Microvesicular Steatosis misc Hepatocellular Ballooning
topic_unstemmed	misc NASH misc Diffuse Reflectance Spectroscopy misc Macrovesicular Steatosis misc Microvesicular Steatosis misc Hepatocellular Ballooning
topic_browse	misc NASH misc Diffuse Reflectance Spectroscopy misc Macrovesicular Steatosis misc Microvesicular Steatosis misc Hepatocellular Ballooning
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title	Diffuse reflectance spectroscopy accurately quantifies various degrees of liver steatosis in murine models of fatty liver disease
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title_full	Diffuse reflectance spectroscopy accurately quantifies various degrees of liver steatosis in murine models of fatty liver disease
author_sort	Westerkamp, Andrie C.
journal	Journal of translational medicine
journalStr	Journal of translational medicine
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author_browse	Westerkamp, Andrie C. Pully, Vishnu V. Karimian, Golnar Bomfati, Fernanda Veldhuis, Zwanida J. Wiersema-Buist, Janneke Hendriks, Benno H. W. Lisman, Ton Porte, Robert J.
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author-letter	Westerkamp, Andrie C.
doi_str_mv	10.1186/s12967-015-0671-1
title_sort	diffuse reflectance spectroscopy accurately quantifies various degrees of liver steatosis in murine models of fatty liver disease
title_auth	Diffuse reflectance spectroscopy accurately quantifies various degrees of liver steatosis in murine models of fatty liver disease
abstract	Background A real-time objective evaluation for the extent of liver steatosis during liver transplantation is currently not available. Diffuse reflectance spectroscopy (DRS) rapidly and accurately assesses the extent of steatosis in human livers with mild steatosis. However, it is yet unknown whether DRS accurately quantifies moderate/severe steatosis and is able to distinguish between micro- and macrovesicular steatosis. Methods C57BL/6JolaHsd mice were fed wit a choline-deficient l-amino acid-defined diet (CD-AA) or a choline-sufficient l-amino acid-defined control diet (CS-AA) for 3, 8, and 20 weeks. In addition B6.V-Lepob/OlaHsd (ob/ob) mice and their lean controls were studied. A total of 104 DRS measurements were performed in liver tissue ex vivo. The degree of steatosis was quantified from the DRS data and compared with histopathological analysis. Results When assessed by histology, livers of mice fed with a CD-AA and CS-AA diet displayed macrovesicular steatosis (range 0–74 %), ob/ob mice revealed only microvesicular steatosis (range 75–80 %), and their lean controls showed no steatosis. The quantification of steatosis by DRS correlated well with pathology (correlation of 0.76 in CD-AA/CS-AA fed mice and a correlation of 0.75 in ob/ob mice). DRS spectra did not distinguish between micro- and macrovesicular steatosis. In samples from CD-AA/CS-AA fed mice, the DRS was able to distinguish between mild and moderate/severe steatosis with a sensitivity and specificity of 86 and 81 %, respectively. Conclusion DRS can quantify steatosis with good agreement to histopathological analysis. DRS may be useful for real-time objective evaluation of liver steatosis during liver transplantation, especially to differentiate between mild and moderate/severe steatosis. © Westerkamp et al. 2015
abstractGer	Background A real-time objective evaluation for the extent of liver steatosis during liver transplantation is currently not available. Diffuse reflectance spectroscopy (DRS) rapidly and accurately assesses the extent of steatosis in human livers with mild steatosis. However, it is yet unknown whether DRS accurately quantifies moderate/severe steatosis and is able to distinguish between micro- and macrovesicular steatosis. Methods C57BL/6JolaHsd mice were fed wit a choline-deficient l-amino acid-defined diet (CD-AA) or a choline-sufficient l-amino acid-defined control diet (CS-AA) for 3, 8, and 20 weeks. In addition B6.V-Lepob/OlaHsd (ob/ob) mice and their lean controls were studied. A total of 104 DRS measurements were performed in liver tissue ex vivo. The degree of steatosis was quantified from the DRS data and compared with histopathological analysis. Results When assessed by histology, livers of mice fed with a CD-AA and CS-AA diet displayed macrovesicular steatosis (range 0–74 %), ob/ob mice revealed only microvesicular steatosis (range 75–80 %), and their lean controls showed no steatosis. The quantification of steatosis by DRS correlated well with pathology (correlation of 0.76 in CD-AA/CS-AA fed mice and a correlation of 0.75 in ob/ob mice). DRS spectra did not distinguish between micro- and macrovesicular steatosis. In samples from CD-AA/CS-AA fed mice, the DRS was able to distinguish between mild and moderate/severe steatosis with a sensitivity and specificity of 86 and 81 %, respectively. Conclusion DRS can quantify steatosis with good agreement to histopathological analysis. DRS may be useful for real-time objective evaluation of liver steatosis during liver transplantation, especially to differentiate between mild and moderate/severe steatosis. © Westerkamp et al. 2015
abstract_unstemmed	Background A real-time objective evaluation for the extent of liver steatosis during liver transplantation is currently not available. Diffuse reflectance spectroscopy (DRS) rapidly and accurately assesses the extent of steatosis in human livers with mild steatosis. However, it is yet unknown whether DRS accurately quantifies moderate/severe steatosis and is able to distinguish between micro- and macrovesicular steatosis. Methods C57BL/6JolaHsd mice were fed wit a choline-deficient l-amino acid-defined diet (CD-AA) or a choline-sufficient l-amino acid-defined control diet (CS-AA) for 3, 8, and 20 weeks. In addition B6.V-Lepob/OlaHsd (ob/ob) mice and their lean controls were studied. A total of 104 DRS measurements were performed in liver tissue ex vivo. The degree of steatosis was quantified from the DRS data and compared with histopathological analysis. Results When assessed by histology, livers of mice fed with a CD-AA and CS-AA diet displayed macrovesicular steatosis (range 0–74 %), ob/ob mice revealed only microvesicular steatosis (range 75–80 %), and their lean controls showed no steatosis. The quantification of steatosis by DRS correlated well with pathology (correlation of 0.76 in CD-AA/CS-AA fed mice and a correlation of 0.75 in ob/ob mice). DRS spectra did not distinguish between micro- and macrovesicular steatosis. In samples from CD-AA/CS-AA fed mice, the DRS was able to distinguish between mild and moderate/severe steatosis with a sensitivity and specificity of 86 and 81 %, respectively. Conclusion DRS can quantify steatosis with good agreement to histopathological analysis. DRS may be useful for real-time objective evaluation of liver steatosis during liver transplantation, especially to differentiate between mild and moderate/severe steatosis. © Westerkamp et al. 2015
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title_short	Diffuse reflectance spectroscopy accurately quantifies various degrees of liver steatosis in murine models of fatty liver disease
url	https://dx.doi.org/10.1186/s12967-015-0671-1
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author2	Pully, Vishnu V. Karimian, Golnar Bomfati, Fernanda Veldhuis, Zwanida J. Wiersema-Buist, Janneke Hendriks, Benno H. W. Lisman, Ton Porte, Robert J.
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up_date	2024-07-03T22:44:30.423Z
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