Variance in multiplex suspension array assays: A distribution generation machine for multiplex counts

Background This study attempted to replicate Luminex experimental results for large numbers of beads per classifier using multiplexed assays and routine instrument use conditions. Conclusion Using larger numbers of microspheres per classifier highlights a fundamental stochastic distribution of bead...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Hanley, Brian P [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2008

Schlagwörter:	Swimming Pool Multinomial Distribution Multiplex Assay Assay Bead Bead Count

Anmerkung:	© Hanley; licensee BioMed Central Ltd. 2008

Übergeordnetes Werk:	Enthalten in: Theoretical biology and medical modelling - London : BioMed Central, 2004, 5(2008), 1 vom: 28. Jan.
Übergeordnetes Werk:	volume:5 ; year:2008 ; number:1 ; day:28 ; month:01

Links:	Volltext

DOI / URN:	10.1186/1742-4682-5-3

Katalog-ID:	SPR029126576

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allfields	10.1186/1742-4682-5-3 doi (DE-627)SPR029126576 (SPR)1742-4682-5-3-e DE-627 ger DE-627 rakwb eng Hanley, Brian P verfasserin aut Variance in multiplex suspension array assays: A distribution generation machine for multiplex counts 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Hanley; licensee BioMed Central Ltd. 2008 Background This study attempted to replicate Luminex experimental results for large numbers of beads per classifier using multiplexed assays and routine instrument use conditions. Conclusion Using larger numbers of microspheres per classifier highlights a fundamental stochastic distribution of bead counts issue complicated by other factors. The more classifiers and the higher the count required per classifier there are, the more apparent the distribution of counts per classifier will be, and the more microspheres are required. Additional problems have been identified. Alternate methods of improving precision and reliability are recommended such as intraplexing and multi-well sample replicates to improve precision and confidence. Swimming Pool (dpeaa)DE-He213 Multinomial Distribution (dpeaa)DE-He213 Multiplex Assay (dpeaa)DE-He213 Assay Bead (dpeaa)DE-He213 Bead Count (dpeaa)DE-He213 Enthalten in Theoretical biology and medical modelling London : BioMed Central, 2004 5(2008), 1 vom: 28. Jan. (DE-627)39178482X (DE-600)2156462-0 1742-4682 nnns volume:5 year:2008 number:1 day:28 month:01 https://dx.doi.org/10.1186/1742-4682-5-3 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 5 2008 1 28 01
spelling	10.1186/1742-4682-5-3 doi (DE-627)SPR029126576 (SPR)1742-4682-5-3-e DE-627 ger DE-627 rakwb eng Hanley, Brian P verfasserin aut Variance in multiplex suspension array assays: A distribution generation machine for multiplex counts 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Hanley; licensee BioMed Central Ltd. 2008 Background This study attempted to replicate Luminex experimental results for large numbers of beads per classifier using multiplexed assays and routine instrument use conditions. Conclusion Using larger numbers of microspheres per classifier highlights a fundamental stochastic distribution of bead counts issue complicated by other factors. The more classifiers and the higher the count required per classifier there are, the more apparent the distribution of counts per classifier will be, and the more microspheres are required. Additional problems have been identified. Alternate methods of improving precision and reliability are recommended such as intraplexing and multi-well sample replicates to improve precision and confidence. Swimming Pool (dpeaa)DE-He213 Multinomial Distribution (dpeaa)DE-He213 Multiplex Assay (dpeaa)DE-He213 Assay Bead (dpeaa)DE-He213 Bead Count (dpeaa)DE-He213 Enthalten in Theoretical biology and medical modelling London : BioMed Central, 2004 5(2008), 1 vom: 28. Jan. (DE-627)39178482X (DE-600)2156462-0 1742-4682 nnns volume:5 year:2008 number:1 day:28 month:01 https://dx.doi.org/10.1186/1742-4682-5-3 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 5 2008 1 28 01
allfields_unstemmed	10.1186/1742-4682-5-3 doi (DE-627)SPR029126576 (SPR)1742-4682-5-3-e DE-627 ger DE-627 rakwb eng Hanley, Brian P verfasserin aut Variance in multiplex suspension array assays: A distribution generation machine for multiplex counts 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Hanley; licensee BioMed Central Ltd. 2008 Background This study attempted to replicate Luminex experimental results for large numbers of beads per classifier using multiplexed assays and routine instrument use conditions. Conclusion Using larger numbers of microspheres per classifier highlights a fundamental stochastic distribution of bead counts issue complicated by other factors. The more classifiers and the higher the count required per classifier there are, the more apparent the distribution of counts per classifier will be, and the more microspheres are required. Additional problems have been identified. Alternate methods of improving precision and reliability are recommended such as intraplexing and multi-well sample replicates to improve precision and confidence. Swimming Pool (dpeaa)DE-He213 Multinomial Distribution (dpeaa)DE-He213 Multiplex Assay (dpeaa)DE-He213 Assay Bead (dpeaa)DE-He213 Bead Count (dpeaa)DE-He213 Enthalten in Theoretical biology and medical modelling London : BioMed Central, 2004 5(2008), 1 vom: 28. Jan. (DE-627)39178482X (DE-600)2156462-0 1742-4682 nnns volume:5 year:2008 number:1 day:28 month:01 https://dx.doi.org/10.1186/1742-4682-5-3 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 5 2008 1 28 01
allfieldsGer	10.1186/1742-4682-5-3 doi (DE-627)SPR029126576 (SPR)1742-4682-5-3-e DE-627 ger DE-627 rakwb eng Hanley, Brian P verfasserin aut Variance in multiplex suspension array assays: A distribution generation machine for multiplex counts 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Hanley; licensee BioMed Central Ltd. 2008 Background This study attempted to replicate Luminex experimental results for large numbers of beads per classifier using multiplexed assays and routine instrument use conditions. Conclusion Using larger numbers of microspheres per classifier highlights a fundamental stochastic distribution of bead counts issue complicated by other factors. The more classifiers and the higher the count required per classifier there are, the more apparent the distribution of counts per classifier will be, and the more microspheres are required. Additional problems have been identified. Alternate methods of improving precision and reliability are recommended such as intraplexing and multi-well sample replicates to improve precision and confidence. Swimming Pool (dpeaa)DE-He213 Multinomial Distribution (dpeaa)DE-He213 Multiplex Assay (dpeaa)DE-He213 Assay Bead (dpeaa)DE-He213 Bead Count (dpeaa)DE-He213 Enthalten in Theoretical biology and medical modelling London : BioMed Central, 2004 5(2008), 1 vom: 28. Jan. (DE-627)39178482X (DE-600)2156462-0 1742-4682 nnns volume:5 year:2008 number:1 day:28 month:01 https://dx.doi.org/10.1186/1742-4682-5-3 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 5 2008 1 28 01
allfieldsSound	10.1186/1742-4682-5-3 doi (DE-627)SPR029126576 (SPR)1742-4682-5-3-e DE-627 ger DE-627 rakwb eng Hanley, Brian P verfasserin aut Variance in multiplex suspension array assays: A distribution generation machine for multiplex counts 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Hanley; licensee BioMed Central Ltd. 2008 Background This study attempted to replicate Luminex experimental results for large numbers of beads per classifier using multiplexed assays and routine instrument use conditions. Conclusion Using larger numbers of microspheres per classifier highlights a fundamental stochastic distribution of bead counts issue complicated by other factors. The more classifiers and the higher the count required per classifier there are, the more apparent the distribution of counts per classifier will be, and the more microspheres are required. Additional problems have been identified. Alternate methods of improving precision and reliability are recommended such as intraplexing and multi-well sample replicates to improve precision and confidence. Swimming Pool (dpeaa)DE-He213 Multinomial Distribution (dpeaa)DE-He213 Multiplex Assay (dpeaa)DE-He213 Assay Bead (dpeaa)DE-He213 Bead Count (dpeaa)DE-He213 Enthalten in Theoretical biology and medical modelling London : BioMed Central, 2004 5(2008), 1 vom: 28. Jan. (DE-627)39178482X (DE-600)2156462-0 1742-4682 nnns volume:5 year:2008 number:1 day:28 month:01 https://dx.doi.org/10.1186/1742-4682-5-3 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 5 2008 1 28 01
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author	Hanley, Brian P
spellingShingle	Hanley, Brian P misc Swimming Pool misc Multinomial Distribution misc Multiplex Assay misc Assay Bead misc Bead Count Variance in multiplex suspension array assays: A distribution generation machine for multiplex counts
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topic_title	Variance in multiplex suspension array assays: A distribution generation machine for multiplex counts Swimming Pool (dpeaa)DE-He213 Multinomial Distribution (dpeaa)DE-He213 Multiplex Assay (dpeaa)DE-He213 Assay Bead (dpeaa)DE-He213 Bead Count (dpeaa)DE-He213
topic	misc Swimming Pool misc Multinomial Distribution misc Multiplex Assay misc Assay Bead misc Bead Count
topic_unstemmed	misc Swimming Pool misc Multinomial Distribution misc Multiplex Assay misc Assay Bead misc Bead Count
topic_browse	misc Swimming Pool misc Multinomial Distribution misc Multiplex Assay misc Assay Bead misc Bead Count
format_facet	Elektronische Aufsätze Aufsätze Elektronische Ressource
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title	Variance in multiplex suspension array assays: A distribution generation machine for multiplex counts
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title_full	Variance in multiplex suspension array assays: A distribution generation machine for multiplex counts
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title_sort	variance in multiplex suspension array assays: a distribution generation machine for multiplex counts
title_auth	Variance in multiplex suspension array assays: A distribution generation machine for multiplex counts
abstract	Background This study attempted to replicate Luminex experimental results for large numbers of beads per classifier using multiplexed assays and routine instrument use conditions. Conclusion Using larger numbers of microspheres per classifier highlights a fundamental stochastic distribution of bead counts issue complicated by other factors. The more classifiers and the higher the count required per classifier there are, the more apparent the distribution of counts per classifier will be, and the more microspheres are required. Additional problems have been identified. Alternate methods of improving precision and reliability are recommended such as intraplexing and multi-well sample replicates to improve precision and confidence. © Hanley; licensee BioMed Central Ltd. 2008
abstractGer	Background This study attempted to replicate Luminex experimental results for large numbers of beads per classifier using multiplexed assays and routine instrument use conditions. Conclusion Using larger numbers of microspheres per classifier highlights a fundamental stochastic distribution of bead counts issue complicated by other factors. The more classifiers and the higher the count required per classifier there are, the more apparent the distribution of counts per classifier will be, and the more microspheres are required. Additional problems have been identified. Alternate methods of improving precision and reliability are recommended such as intraplexing and multi-well sample replicates to improve precision and confidence. © Hanley; licensee BioMed Central Ltd. 2008
abstract_unstemmed	Background This study attempted to replicate Luminex experimental results for large numbers of beads per classifier using multiplexed assays and routine instrument use conditions. Conclusion Using larger numbers of microspheres per classifier highlights a fundamental stochastic distribution of bead counts issue complicated by other factors. The more classifiers and the higher the count required per classifier there are, the more apparent the distribution of counts per classifier will be, and the more microspheres are required. Additional problems have been identified. Alternate methods of improving precision and reliability are recommended such as intraplexing and multi-well sample replicates to improve precision and confidence. © Hanley; licensee BioMed Central Ltd. 2008
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title_short	Variance in multiplex suspension array assays: A distribution generation machine for multiplex counts
url	https://dx.doi.org/10.1186/1742-4682-5-3
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up_date	2024-07-03T23:36:49.144Z
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