The novel HSV-1 $ U_{S} $5-1 RNA is transcribed off a domain encoding $ U_{S} $ 5, $ U_{S} $ 4, $ U_{S} $ 3, $ U_{S} $ 2 and α22

Background The genome of herpes simplex virus 1 encodes at least 84 transcripts from which proteins are translated and several additional RNAs whose status as mRNAs is unknown. These RNAs include latency-associated transcript, $ Ori_{S} $1 and $ Ori_{S} $2 RNAs and in case of α4 null mutant addition...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Jovasevic, Vladimir [verfasserIn] Roizman, Bernard

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2010

Schlagwörter:	Northern Blot Analysis RNase Protection Assay Northern Blot Experiment Protected Fragment Unique Short

Anmerkung:	© Jovasevic and Roizman; licensee BioMed Central Ltd. 2010

Übergeordnetes Werk:	Enthalten in: Virology journal - London : BioMed Central, 2004, 7(2010), 1 vom: 21. Mai
Übergeordnetes Werk:	volume:7 ; year:2010 ; number:1 ; day:21 ; month:05

Links:	Volltext

DOI / URN:	10.1186/1743-422X-7-103

Katalog-ID:	SPR029241820

Internformat


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040			\|a DE-627 \|b ger \|c DE-627 \|e rakwb
041			\|a eng
100	1		\|a Jovasevic, Vladimir \|e verfasserin \|4 aut
245	1	4	\|a The novel HSV-1 $ U_{S} $5-1 RNA is transcribed off a domain encoding $ U_{S} $ 5, $ U_{S} $ 4, $ U_{S} $ 3, $ U_{S} $ 2 and α22
264		1	\|c 2010
336			\|a Text \|b txt \|2 rdacontent
337			\|a Computermedien \|b c \|2 rdamedia
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500			\|a © Jovasevic and Roizman; licensee BioMed Central Ltd. 2010
520			\|a Background The genome of herpes simplex virus 1 encodes at least 84 transcripts from which proteins are translated and several additional RNAs whose status as mRNAs is unknown. These RNAs include latency-associated transcript, $ Ori_{S} $1 and $ Ori_{S} $2 RNAs and in case of α4 null mutant additional transcript that spans the junction between L and S component of the HSV-1 genome. Current data do not suggest that a peptide is translated from these RNAs. Results We describe here a novel RNA designated $ U_{S} $5-1 that spans 4.5 kb of the unique-short ($ U_{S} $) region. The RNA initiates in $ U_{S} $5 and terminates in the α22 open reading frame. It is expressed antisense to $ U_{S} $5, $ U_{S} $4, $ U_{S} $3 and ICP22 mRNAs. This transcript is expressed with $ γ_{2} $ kinetics and has a half-life of 80 minutes. Conclusion These results identify a novel transcript encoded within HSV-1 genome. Since no major hypothetical open-reading frames are present in this transcript it is feasible that this RNA exerts its function as a non-coding RNA.
650		4	\|a Northern Blot Analysis \|7 (dpeaa)DE-He213
650		4	\|a RNase Protection Assay \|7 (dpeaa)DE-He213
650		4	\|a Northern Blot Experiment \|7 (dpeaa)DE-He213
650		4	\|a Protected Fragment \|7 (dpeaa)DE-He213
650		4	\|a Unique Short \|7 (dpeaa)DE-He213
700	1		\|a Roizman, Bernard \|4 aut
773	0	8	\|i Enthalten in \|t Virology journal \|d London : BioMed Central, 2004 \|g 7(2010), 1 vom: 21. Mai \|w (DE-627)394165004 \|w (DE-600)2160640-7 \|x 1743-422X \|7 nnns
773	1	8	\|g volume:7 \|g year:2010 \|g number:1 \|g day:21 \|g month:05
856	4	0	\|u https://dx.doi.org/10.1186/1743-422X-7-103 \|z kostenfrei \|3 Volltext
912			\|a GBV_USEFLAG_A
912			\|a SYSFLAG_A
912			\|a GBV_SPRINGER
912			\|a SSG-OLC-PHA
912			\|a GBV_ILN_11
912			\|a GBV_ILN_20
912			\|a GBV_ILN_22
912			\|a GBV_ILN_23
912			\|a GBV_ILN_24
912			\|a GBV_ILN_31
912			\|a GBV_ILN_39
912			\|a GBV_ILN_40
912			\|a GBV_ILN_60
912			\|a GBV_ILN_62
912			\|a GBV_ILN_63
912			\|a GBV_ILN_65
912			\|a GBV_ILN_69
912			\|a GBV_ILN_73
912			\|a GBV_ILN_74
912			\|a GBV_ILN_95
912			\|a GBV_ILN_105
912			\|a GBV_ILN_110
912			\|a GBV_ILN_151
912			\|a GBV_ILN_161
912			\|a GBV_ILN_170
912			\|a GBV_ILN_206
912			\|a GBV_ILN_213
912			\|a GBV_ILN_230
912			\|a GBV_ILN_285
912			\|a GBV_ILN_293
912			\|a GBV_ILN_602
912			\|a GBV_ILN_702
912			\|a GBV_ILN_2001
912			\|a GBV_ILN_2003
912			\|a GBV_ILN_2005
912			\|a GBV_ILN_2006
912			\|a GBV_ILN_2008
912			\|a GBV_ILN_2009
912			\|a GBV_ILN_2010
912			\|a GBV_ILN_2011
912			\|a GBV_ILN_2014
912			\|a GBV_ILN_2015
912			\|a GBV_ILN_2020
912			\|a GBV_ILN_2021
912			\|a GBV_ILN_2025
912			\|a GBV_ILN_2031
912			\|a GBV_ILN_2038
912			\|a GBV_ILN_2044
912			\|a GBV_ILN_2048
912			\|a GBV_ILN_2050
912			\|a GBV_ILN_2055
912			\|a GBV_ILN_2056
912			\|a GBV_ILN_2057
912			\|a GBV_ILN_2061
912			\|a GBV_ILN_2111
912			\|a GBV_ILN_2113
912			\|a GBV_ILN_2190
912			\|a GBV_ILN_4012
912			\|a GBV_ILN_4037
912			\|a GBV_ILN_4112
912			\|a GBV_ILN_4125
912			\|a GBV_ILN_4126
912			\|a GBV_ILN_4249
912			\|a GBV_ILN_4305
912			\|a GBV_ILN_4306
912			\|a GBV_ILN_4307
912			\|a GBV_ILN_4313
912			\|a GBV_ILN_4322
912			\|a GBV_ILN_4323
912			\|a GBV_ILN_4324
912			\|a GBV_ILN_4325
912			\|a GBV_ILN_4338
912			\|a GBV_ILN_4367
912			\|a GBV_ILN_4700
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952			\|d 7 \|j 2010 \|e 1 \|b 21 \|c 05

Indexfelder

author_variant	v j vj b r br
matchkey_str	article:1743422X:2010----::hnvls1_5rasrncieofdmiecdnus
hierarchy_sort_str	2010
publishDate	2010
allfields	10.1186/1743-422X-7-103 doi (DE-627)SPR029241820 (SPR)1743-422X-7-103-e DE-627 ger DE-627 rakwb eng Jovasevic, Vladimir verfasserin aut The novel HSV-1 $ U_{S} $5-1 RNA is transcribed off a domain encoding $ U_{S} $ 5, $ U_{S} $ 4, $ U_{S} $ 3, $ U_{S} $ 2 and α22 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Jovasevic and Roizman; licensee BioMed Central Ltd. 2010 Background The genome of herpes simplex virus 1 encodes at least 84 transcripts from which proteins are translated and several additional RNAs whose status as mRNAs is unknown. These RNAs include latency-associated transcript, $ Ori_{S} $1 and $ Ori_{S} $2 RNAs and in case of α4 null mutant additional transcript that spans the junction between L and S component of the HSV-1 genome. Current data do not suggest that a peptide is translated from these RNAs. Results We describe here a novel RNA designated $ U_{S} $5-1 that spans 4.5 kb of the unique-short ($ U_{S} $) region. The RNA initiates in $ U_{S} $5 and terminates in the α22 open reading frame. It is expressed antisense to $ U_{S} $5, $ U_{S} $4, $ U_{S} $3 and ICP22 mRNAs. This transcript is expressed with $ γ_{2} $ kinetics and has a half-life of 80 minutes. Conclusion These results identify a novel transcript encoded within HSV-1 genome. Since no major hypothetical open-reading frames are present in this transcript it is feasible that this RNA exerts its function as a non-coding RNA. Northern Blot Analysis (dpeaa)DE-He213 RNase Protection Assay (dpeaa)DE-He213 Northern Blot Experiment (dpeaa)DE-He213 Protected Fragment (dpeaa)DE-He213 Unique Short (dpeaa)DE-He213 Roizman, Bernard aut Enthalten in Virology journal London : BioMed Central, 2004 7(2010), 1 vom: 21. Mai (DE-627)394165004 (DE-600)2160640-7 1743-422X nnns volume:7 year:2010 number:1 day:21 month:05 https://dx.doi.org/10.1186/1743-422X-7-103 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2010 1 21 05
spelling	10.1186/1743-422X-7-103 doi (DE-627)SPR029241820 (SPR)1743-422X-7-103-e DE-627 ger DE-627 rakwb eng Jovasevic, Vladimir verfasserin aut The novel HSV-1 $ U_{S} $5-1 RNA is transcribed off a domain encoding $ U_{S} $ 5, $ U_{S} $ 4, $ U_{S} $ 3, $ U_{S} $ 2 and α22 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Jovasevic and Roizman; licensee BioMed Central Ltd. 2010 Background The genome of herpes simplex virus 1 encodes at least 84 transcripts from which proteins are translated and several additional RNAs whose status as mRNAs is unknown. These RNAs include latency-associated transcript, $ Ori_{S} $1 and $ Ori_{S} $2 RNAs and in case of α4 null mutant additional transcript that spans the junction between L and S component of the HSV-1 genome. Current data do not suggest that a peptide is translated from these RNAs. Results We describe here a novel RNA designated $ U_{S} $5-1 that spans 4.5 kb of the unique-short ($ U_{S} $) region. The RNA initiates in $ U_{S} $5 and terminates in the α22 open reading frame. It is expressed antisense to $ U_{S} $5, $ U_{S} $4, $ U_{S} $3 and ICP22 mRNAs. This transcript is expressed with $ γ_{2} $ kinetics and has a half-life of 80 minutes. Conclusion These results identify a novel transcript encoded within HSV-1 genome. Since no major hypothetical open-reading frames are present in this transcript it is feasible that this RNA exerts its function as a non-coding RNA. Northern Blot Analysis (dpeaa)DE-He213 RNase Protection Assay (dpeaa)DE-He213 Northern Blot Experiment (dpeaa)DE-He213 Protected Fragment (dpeaa)DE-He213 Unique Short (dpeaa)DE-He213 Roizman, Bernard aut Enthalten in Virology journal London : BioMed Central, 2004 7(2010), 1 vom: 21. Mai (DE-627)394165004 (DE-600)2160640-7 1743-422X nnns volume:7 year:2010 number:1 day:21 month:05 https://dx.doi.org/10.1186/1743-422X-7-103 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2010 1 21 05
allfields_unstemmed	10.1186/1743-422X-7-103 doi (DE-627)SPR029241820 (SPR)1743-422X-7-103-e DE-627 ger DE-627 rakwb eng Jovasevic, Vladimir verfasserin aut The novel HSV-1 $ U_{S} $5-1 RNA is transcribed off a domain encoding $ U_{S} $ 5, $ U_{S} $ 4, $ U_{S} $ 3, $ U_{S} $ 2 and α22 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Jovasevic and Roizman; licensee BioMed Central Ltd. 2010 Background The genome of herpes simplex virus 1 encodes at least 84 transcripts from which proteins are translated and several additional RNAs whose status as mRNAs is unknown. These RNAs include latency-associated transcript, $ Ori_{S} $1 and $ Ori_{S} $2 RNAs and in case of α4 null mutant additional transcript that spans the junction between L and S component of the HSV-1 genome. Current data do not suggest that a peptide is translated from these RNAs. Results We describe here a novel RNA designated $ U_{S} $5-1 that spans 4.5 kb of the unique-short ($ U_{S} $) region. The RNA initiates in $ U_{S} $5 and terminates in the α22 open reading frame. It is expressed antisense to $ U_{S} $5, $ U_{S} $4, $ U_{S} $3 and ICP22 mRNAs. This transcript is expressed with $ γ_{2} $ kinetics and has a half-life of 80 minutes. Conclusion These results identify a novel transcript encoded within HSV-1 genome. Since no major hypothetical open-reading frames are present in this transcript it is feasible that this RNA exerts its function as a non-coding RNA. Northern Blot Analysis (dpeaa)DE-He213 RNase Protection Assay (dpeaa)DE-He213 Northern Blot Experiment (dpeaa)DE-He213 Protected Fragment (dpeaa)DE-He213 Unique Short (dpeaa)DE-He213 Roizman, Bernard aut Enthalten in Virology journal London : BioMed Central, 2004 7(2010), 1 vom: 21. Mai (DE-627)394165004 (DE-600)2160640-7 1743-422X nnns volume:7 year:2010 number:1 day:21 month:05 https://dx.doi.org/10.1186/1743-422X-7-103 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2010 1 21 05
allfieldsGer	10.1186/1743-422X-7-103 doi (DE-627)SPR029241820 (SPR)1743-422X-7-103-e DE-627 ger DE-627 rakwb eng Jovasevic, Vladimir verfasserin aut The novel HSV-1 $ U_{S} $5-1 RNA is transcribed off a domain encoding $ U_{S} $ 5, $ U_{S} $ 4, $ U_{S} $ 3, $ U_{S} $ 2 and α22 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Jovasevic and Roizman; licensee BioMed Central Ltd. 2010 Background The genome of herpes simplex virus 1 encodes at least 84 transcripts from which proteins are translated and several additional RNAs whose status as mRNAs is unknown. These RNAs include latency-associated transcript, $ Ori_{S} $1 and $ Ori_{S} $2 RNAs and in case of α4 null mutant additional transcript that spans the junction between L and S component of the HSV-1 genome. Current data do not suggest that a peptide is translated from these RNAs. Results We describe here a novel RNA designated $ U_{S} $5-1 that spans 4.5 kb of the unique-short ($ U_{S} $) region. The RNA initiates in $ U_{S} $5 and terminates in the α22 open reading frame. It is expressed antisense to $ U_{S} $5, $ U_{S} $4, $ U_{S} $3 and ICP22 mRNAs. This transcript is expressed with $ γ_{2} $ kinetics and has a half-life of 80 minutes. Conclusion These results identify a novel transcript encoded within HSV-1 genome. Since no major hypothetical open-reading frames are present in this transcript it is feasible that this RNA exerts its function as a non-coding RNA. Northern Blot Analysis (dpeaa)DE-He213 RNase Protection Assay (dpeaa)DE-He213 Northern Blot Experiment (dpeaa)DE-He213 Protected Fragment (dpeaa)DE-He213 Unique Short (dpeaa)DE-He213 Roizman, Bernard aut Enthalten in Virology journal London : BioMed Central, 2004 7(2010), 1 vom: 21. Mai (DE-627)394165004 (DE-600)2160640-7 1743-422X nnns volume:7 year:2010 number:1 day:21 month:05 https://dx.doi.org/10.1186/1743-422X-7-103 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2010 1 21 05
allfieldsSound	10.1186/1743-422X-7-103 doi (DE-627)SPR029241820 (SPR)1743-422X-7-103-e DE-627 ger DE-627 rakwb eng Jovasevic, Vladimir verfasserin aut The novel HSV-1 $ U_{S} $5-1 RNA is transcribed off a domain encoding $ U_{S} $ 5, $ U_{S} $ 4, $ U_{S} $ 3, $ U_{S} $ 2 and α22 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Jovasevic and Roizman; licensee BioMed Central Ltd. 2010 Background The genome of herpes simplex virus 1 encodes at least 84 transcripts from which proteins are translated and several additional RNAs whose status as mRNAs is unknown. These RNAs include latency-associated transcript, $ Ori_{S} $1 and $ Ori_{S} $2 RNAs and in case of α4 null mutant additional transcript that spans the junction between L and S component of the HSV-1 genome. Current data do not suggest that a peptide is translated from these RNAs. Results We describe here a novel RNA designated $ U_{S} $5-1 that spans 4.5 kb of the unique-short ($ U_{S} $) region. The RNA initiates in $ U_{S} $5 and terminates in the α22 open reading frame. It is expressed antisense to $ U_{S} $5, $ U_{S} $4, $ U_{S} $3 and ICP22 mRNAs. This transcript is expressed with $ γ_{2} $ kinetics and has a half-life of 80 minutes. Conclusion These results identify a novel transcript encoded within HSV-1 genome. Since no major hypothetical open-reading frames are present in this transcript it is feasible that this RNA exerts its function as a non-coding RNA. Northern Blot Analysis (dpeaa)DE-He213 RNase Protection Assay (dpeaa)DE-He213 Northern Blot Experiment (dpeaa)DE-He213 Protected Fragment (dpeaa)DE-He213 Unique Short (dpeaa)DE-He213 Roizman, Bernard aut Enthalten in Virology journal London : BioMed Central, 2004 7(2010), 1 vom: 21. Mai (DE-627)394165004 (DE-600)2160640-7 1743-422X nnns volume:7 year:2010 number:1 day:21 month:05 https://dx.doi.org/10.1186/1743-422X-7-103 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2010 1 21 05
language	English
source	Enthalten in Virology journal 7(2010), 1 vom: 21. Mai volume:7 year:2010 number:1 day:21 month:05
sourceStr	Enthalten in Virology journal 7(2010), 1 vom: 21. Mai volume:7 year:2010 number:1 day:21 month:05
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These RNAs include latency-associated transcript, $ Ori_{S} $1 and $ Ori_{S} $2 RNAs and in case of α4 null mutant additional transcript that spans the junction between L and S component of the HSV-1 genome. Current data do not suggest that a peptide is translated from these RNAs. Results We describe here a novel RNA designated $ U_{S} $5-1 that spans 4.5 kb of the unique-short ($ U_{S} $) region. The RNA initiates in $ U_{S} $5 and terminates in the α22 open reading frame. It is expressed antisense to $ U_{S} $5, $ U_{S} $4, $ U_{S} $3 and ICP22 mRNAs. This transcript is expressed with $ γ_{2} $ kinetics and has a half-life of 80 minutes. Conclusion These results identify a novel transcript encoded within HSV-1 genome. 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author	Jovasevic, Vladimir
spellingShingle	Jovasevic, Vladimir misc Northern Blot Analysis misc RNase Protection Assay misc Northern Blot Experiment misc Protected Fragment misc Unique Short The novel HSV-1 $ U_{S} $5-1 RNA is transcribed off a domain encoding $ U_{S} $ 5, $ U_{S} $ 4, $ U_{S} $ 3, $ U_{S} $ 2 and α22
authorStr	Jovasevic, Vladimir
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topic_title	The novel HSV-1 $ U_{S} $5-1 RNA is transcribed off a domain encoding $ U_{S} $ 5, $ U_{S} $ 4, $ U_{S} $ 3, $ U_{S} $ 2 and α22 Northern Blot Analysis (dpeaa)DE-He213 RNase Protection Assay (dpeaa)DE-He213 Northern Blot Experiment (dpeaa)DE-He213 Protected Fragment (dpeaa)DE-He213 Unique Short (dpeaa)DE-He213
topic	misc Northern Blot Analysis misc RNase Protection Assay misc Northern Blot Experiment misc Protected Fragment misc Unique Short
topic_unstemmed	misc Northern Blot Analysis misc RNase Protection Assay misc Northern Blot Experiment misc Protected Fragment misc Unique Short
topic_browse	misc Northern Blot Analysis misc RNase Protection Assay misc Northern Blot Experiment misc Protected Fragment misc Unique Short
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title	The novel HSV-1 $ U_{S} $5-1 RNA is transcribed off a domain encoding $ U_{S} $ 5, $ U_{S} $ 4, $ U_{S} $ 3, $ U_{S} $ 2 and α22
ctrlnum	(DE-627)SPR029241820 (SPR)1743-422X-7-103-e
title_full	The novel HSV-1 $ U_{S} $5-1 RNA is transcribed off a domain encoding $ U_{S} $ 5, $ U_{S} $ 4, $ U_{S} $ 3, $ U_{S} $ 2 and α22
author_sort	Jovasevic, Vladimir
journal	Virology journal
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author_browse	Jovasevic, Vladimir Roizman, Bernard
container_volume	7
format_se	Elektronische Aufsätze
author-letter	Jovasevic, Vladimir
doi_str_mv	10.1186/1743-422X-7-103
title_sort	novel hsv-1 $ u_{s} $5-1 rna is transcribed off a domain encoding $ u_{s} $ 5, $ u_{s} $ 4, $ u_{s} $ 3, $ u_{s} $ 2 and α22
title_auth	The novel HSV-1 $ U_{S} $5-1 RNA is transcribed off a domain encoding $ U_{S} $ 5, $ U_{S} $ 4, $ U_{S} $ 3, $ U_{S} $ 2 and α22
abstract	Background The genome of herpes simplex virus 1 encodes at least 84 transcripts from which proteins are translated and several additional RNAs whose status as mRNAs is unknown. These RNAs include latency-associated transcript, $ Ori_{S} $1 and $ Ori_{S} $2 RNAs and in case of α4 null mutant additional transcript that spans the junction between L and S component of the HSV-1 genome. Current data do not suggest that a peptide is translated from these RNAs. Results We describe here a novel RNA designated $ U_{S} $5-1 that spans 4.5 kb of the unique-short ($ U_{S} $) region. The RNA initiates in $ U_{S} $5 and terminates in the α22 open reading frame. It is expressed antisense to $ U_{S} $5, $ U_{S} $4, $ U_{S} $3 and ICP22 mRNAs. This transcript is expressed with $ γ_{2} $ kinetics and has a half-life of 80 minutes. Conclusion These results identify a novel transcript encoded within HSV-1 genome. Since no major hypothetical open-reading frames are present in this transcript it is feasible that this RNA exerts its function as a non-coding RNA. © Jovasevic and Roizman; licensee BioMed Central Ltd. 2010
abstractGer	Background The genome of herpes simplex virus 1 encodes at least 84 transcripts from which proteins are translated and several additional RNAs whose status as mRNAs is unknown. These RNAs include latency-associated transcript, $ Ori_{S} $1 and $ Ori_{S} $2 RNAs and in case of α4 null mutant additional transcript that spans the junction between L and S component of the HSV-1 genome. Current data do not suggest that a peptide is translated from these RNAs. Results We describe here a novel RNA designated $ U_{S} $5-1 that spans 4.5 kb of the unique-short ($ U_{S} $) region. The RNA initiates in $ U_{S} $5 and terminates in the α22 open reading frame. It is expressed antisense to $ U_{S} $5, $ U_{S} $4, $ U_{S} $3 and ICP22 mRNAs. This transcript is expressed with $ γ_{2} $ kinetics and has a half-life of 80 minutes. Conclusion These results identify a novel transcript encoded within HSV-1 genome. Since no major hypothetical open-reading frames are present in this transcript it is feasible that this RNA exerts its function as a non-coding RNA. © Jovasevic and Roizman; licensee BioMed Central Ltd. 2010
abstract_unstemmed	Background The genome of herpes simplex virus 1 encodes at least 84 transcripts from which proteins are translated and several additional RNAs whose status as mRNAs is unknown. These RNAs include latency-associated transcript, $ Ori_{S} $1 and $ Ori_{S} $2 RNAs and in case of α4 null mutant additional transcript that spans the junction between L and S component of the HSV-1 genome. Current data do not suggest that a peptide is translated from these RNAs. Results We describe here a novel RNA designated $ U_{S} $5-1 that spans 4.5 kb of the unique-short ($ U_{S} $) region. The RNA initiates in $ U_{S} $5 and terminates in the α22 open reading frame. It is expressed antisense to $ U_{S} $5, $ U_{S} $4, $ U_{S} $3 and ICP22 mRNAs. This transcript is expressed with $ γ_{2} $ kinetics and has a half-life of 80 minutes. Conclusion These results identify a novel transcript encoded within HSV-1 genome. Since no major hypothetical open-reading frames are present in this transcript it is feasible that this RNA exerts its function as a non-coding RNA. © Jovasevic and Roizman; licensee BioMed Central Ltd. 2010
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container_issue	1
title_short	The novel HSV-1 $ U_{S} $5-1 RNA is transcribed off a domain encoding $ U_{S} $ 5, $ U_{S} $ 4, $ U_{S} $ 3, $ U_{S} $ 2 and α22
url	https://dx.doi.org/10.1186/1743-422X-7-103
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up_date	2024-07-04T00:07:35.794Z
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fullrecord_marcxml	<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">SPR029241820</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230519233952.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">201007s2010 xx \|\|\|\|\|o 00\| \|\|eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1186/1743-422X-7-103</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)SPR029241820</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(SPR)1743-422X-7-103-e</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Jovasevic, Vladimir</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="4"><subfield code="a">The novel HSV-1 $ U_{S} $5-1 RNA is transcribed off a domain encoding $ U_{S} $ 5, $ U_{S} $ 4, $ U_{S} $ 3, $ U_{S} $ 2 and α22</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2010</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">© Jovasevic and Roizman; licensee BioMed Central Ltd. 2010</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Background The genome of herpes simplex virus 1 encodes at least 84 transcripts from which proteins are translated and several additional RNAs whose status as mRNAs is unknown. These RNAs include latency-associated transcript, $ Ori_{S} $1 and $ Ori_{S} $2 RNAs and in case of α4 null mutant additional transcript that spans the junction between L and S component of the HSV-1 genome. Current data do not suggest that a peptide is translated from these RNAs. Results We describe here a novel RNA designated $ U_{S} $5-1 that spans 4.5 kb of the unique-short ($ U_{S} $) region. The RNA initiates in $ U_{S} $5 and terminates in the α22 open reading frame. It is expressed antisense to $ U_{S} $5, $ U_{S} $4, $ U_{S} $3 and ICP22 mRNAs. This transcript is expressed with $ γ_{2} $ kinetics and has a half-life of 80 minutes. Conclusion These results identify a novel transcript encoded within HSV-1 genome. Since no major hypothetical open-reading frames are present in this transcript it is feasible that this RNA exerts its function as a non-coding RNA.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Northern Blot Analysis</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">RNase Protection Assay</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Northern Blot Experiment</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Protected Fragment</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Unique Short</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Roizman, Bernard</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Virology journal</subfield><subfield code="d">London : BioMed Central, 2004</subfield><subfield code="g">7(2010), 1 vom: 21. 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The novel HSV-1 $ U_{S} $5-1 RNA is transcribed off a domain encoding $ U_{S} $ 5, $ U_{S} $ 4, $ U_{S} $ 3, $ U_{S} $ 2 and α22

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