Comparative evaluation of a laboratory-developed real-time PCR assay and RealStar® Adenovirus PCR Kit for quantitative detection of human adenovirus
Background Human adenoviruses are common causes of community-acquired respiratory tract and enteric infections. Severe disseminated infections with high mortality rates may be seen in immunocompromised individuals. An accurate and cost-effective quantitative assay is essential not only for laborator...
Ausführliche Beschreibung
Autor*in: |
Wong, Samson S. Y. [verfasserIn] |
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E-Artikel |
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Englisch |
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2018 |
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Anmerkung: |
© The Author(s). 2018 |
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Übergeordnetes Werk: |
Enthalten in: Virology journal - London : BioMed Central, 2004, 15(2018), 1 vom: 27. Sept. |
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Übergeordnetes Werk: |
volume:15 ; year:2018 ; number:1 ; day:27 ; month:09 |
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DOI / URN: |
10.1186/s12985-018-1059-7 |
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Katalog-ID: |
SPR029268079 |
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100 | 1 | |a Wong, Samson S. Y. |e verfasserin |4 aut | |
245 | 1 | 0 | |a Comparative evaluation of a laboratory-developed real-time PCR assay and RealStar® Adenovirus PCR Kit for quantitative detection of human adenovirus |
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520 | |a Background Human adenoviruses are common causes of community-acquired respiratory tract and enteric infections. Severe disseminated infections with high mortality rates may be seen in immunocompromised individuals. An accurate and cost-effective quantitative assay is essential not only for laboratory diagnosis of adenoviral infections, but also for monitoring of response to antiviral treatment. The diagnostic performance of an in-house quantitative polymerase chain reaction assay was compared to a commercial system. Methods The analytical sensitivity, specificity, linearity, precision and accuracy of an in-house adenovirus quantitative polymerase chain reaction assay were evaluated against the RealStar® Adenovirus PCR Kit (Altona Diagnostics GmbH, Hamburg, Germany), using 122 clinical specimens and 18 proficiency testing samples. Results Linear regression analysis of the quantitative results by the in-house assay showed the dynamic range from 2.60 to 9 $ log_{10} $ (plasma) and 2.94 to 9 $ log_{10} $ (viral transport medium) copies/mL, with the coefficient of determination ($ R^{2} $) of 0.996 and 0.998, respectively. A dilution series demonstrated the limits of detection and lower limits of quantification for plasma were 2.06 $ log_{10} $ and 2.60 $ log_{10} $ copies/mL and those for viral transport medium were 2.31 $ log_{10} $ and 2.94 $ log_{10} $ copies/mL respectively. The precision of the in-house assay was highly reproducible among runs with coefficients of variance ranging from 0.07 to 3.21% for plasma and 0.17% to 2.11% for viral transport medium. A comparison of 52 matched samples showed an excellent correlation between the quantitative viral loads measured by the in-house assay and the RealStar® Adenovirus PCR Kit ($ R^{2} $ = 0.984), with an average bias of − 0.16 $ log_{10} $ copies/mL. Conclusions The in-house adenovirus assay is a sensitive and reliable assay with lower cost for the detection and quantification of adenoviral DNA when compared to the RealStar® Adenovirus PCR Kit. | ||
650 | 4 | |a Human adenovirus |7 (dpeaa)DE-He213 | |
650 | 4 | |a Real-time PCR |7 (dpeaa)DE-He213 | |
650 | 4 | |a RealStar® Adenovirus PCR Kit |7 (dpeaa)DE-He213 | |
700 | 1 | |a Yip, Cyril C. Y. |4 aut | |
700 | 1 | |a Sridhar, Siddharth |4 aut | |
700 | 1 | |a Leung, Kit-Hang |4 aut | |
700 | 1 | |a Cheng, Andrew K. W. |4 aut | |
700 | 1 | |a Fung, Ami M. Y. |4 aut | |
700 | 1 | |a Lam, Ho-Yin |4 aut | |
700 | 1 | |a Chan, Kwok-Hung |4 aut | |
700 | 1 | |a Chan, Jasper F. W. |4 aut | |
700 | 1 | |a Cheng, Vincent C. C. |4 aut | |
700 | 1 | |a Tang, Bone S. F. |4 aut | |
700 | 1 | |a Yuen, Kwok-Yung |4 aut | |
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912 | |a GBV_ILN_4325 | ||
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10.1186/s12985-018-1059-7 doi (DE-627)SPR029268079 (SPR)s12985-018-1059-7-e DE-627 ger DE-627 rakwb eng Wong, Samson S. Y. verfasserin aut Comparative evaluation of a laboratory-developed real-time PCR assay and RealStar® Adenovirus PCR Kit for quantitative detection of human adenovirus 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2018 Background Human adenoviruses are common causes of community-acquired respiratory tract and enteric infections. Severe disseminated infections with high mortality rates may be seen in immunocompromised individuals. An accurate and cost-effective quantitative assay is essential not only for laboratory diagnosis of adenoviral infections, but also for monitoring of response to antiviral treatment. The diagnostic performance of an in-house quantitative polymerase chain reaction assay was compared to a commercial system. Methods The analytical sensitivity, specificity, linearity, precision and accuracy of an in-house adenovirus quantitative polymerase chain reaction assay were evaluated against the RealStar® Adenovirus PCR Kit (Altona Diagnostics GmbH, Hamburg, Germany), using 122 clinical specimens and 18 proficiency testing samples. Results Linear regression analysis of the quantitative results by the in-house assay showed the dynamic range from 2.60 to 9 $ log_{10} $ (plasma) and 2.94 to 9 $ log_{10} $ (viral transport medium) copies/mL, with the coefficient of determination ($ R^{2} $) of 0.996 and 0.998, respectively. A dilution series demonstrated the limits of detection and lower limits of quantification for plasma were 2.06 $ log_{10} $ and 2.60 $ log_{10} $ copies/mL and those for viral transport medium were 2.31 $ log_{10} $ and 2.94 $ log_{10} $ copies/mL respectively. The precision of the in-house assay was highly reproducible among runs with coefficients of variance ranging from 0.07 to 3.21% for plasma and 0.17% to 2.11% for viral transport medium. A comparison of 52 matched samples showed an excellent correlation between the quantitative viral loads measured by the in-house assay and the RealStar® Adenovirus PCR Kit ($ R^{2} $ = 0.984), with an average bias of − 0.16 $ log_{10} $ copies/mL. Conclusions The in-house adenovirus assay is a sensitive and reliable assay with lower cost for the detection and quantification of adenoviral DNA when compared to the RealStar® Adenovirus PCR Kit. Human adenovirus (dpeaa)DE-He213 Real-time PCR (dpeaa)DE-He213 RealStar® Adenovirus PCR Kit (dpeaa)DE-He213 Yip, Cyril C. Y. aut Sridhar, Siddharth aut Leung, Kit-Hang aut Cheng, Andrew K. W. aut Fung, Ami M. Y. aut Lam, Ho-Yin aut Chan, Kwok-Hung aut Chan, Jasper F. W. aut Cheng, Vincent C. C. aut Tang, Bone S. F. aut Yuen, Kwok-Yung aut Enthalten in Virology journal London : BioMed Central, 2004 15(2018), 1 vom: 27. Sept. (DE-627)394165004 (DE-600)2160640-7 1743-422X nnns volume:15 year:2018 number:1 day:27 month:09 https://dx.doi.org/10.1186/s12985-018-1059-7 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2018 1 27 09 |
spelling |
10.1186/s12985-018-1059-7 doi (DE-627)SPR029268079 (SPR)s12985-018-1059-7-e DE-627 ger DE-627 rakwb eng Wong, Samson S. Y. verfasserin aut Comparative evaluation of a laboratory-developed real-time PCR assay and RealStar® Adenovirus PCR Kit for quantitative detection of human adenovirus 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2018 Background Human adenoviruses are common causes of community-acquired respiratory tract and enteric infections. Severe disseminated infections with high mortality rates may be seen in immunocompromised individuals. An accurate and cost-effective quantitative assay is essential not only for laboratory diagnosis of adenoviral infections, but also for monitoring of response to antiviral treatment. The diagnostic performance of an in-house quantitative polymerase chain reaction assay was compared to a commercial system. Methods The analytical sensitivity, specificity, linearity, precision and accuracy of an in-house adenovirus quantitative polymerase chain reaction assay were evaluated against the RealStar® Adenovirus PCR Kit (Altona Diagnostics GmbH, Hamburg, Germany), using 122 clinical specimens and 18 proficiency testing samples. Results Linear regression analysis of the quantitative results by the in-house assay showed the dynamic range from 2.60 to 9 $ log_{10} $ (plasma) and 2.94 to 9 $ log_{10} $ (viral transport medium) copies/mL, with the coefficient of determination ($ R^{2} $) of 0.996 and 0.998, respectively. A dilution series demonstrated the limits of detection and lower limits of quantification for plasma were 2.06 $ log_{10} $ and 2.60 $ log_{10} $ copies/mL and those for viral transport medium were 2.31 $ log_{10} $ and 2.94 $ log_{10} $ copies/mL respectively. The precision of the in-house assay was highly reproducible among runs with coefficients of variance ranging from 0.07 to 3.21% for plasma and 0.17% to 2.11% for viral transport medium. A comparison of 52 matched samples showed an excellent correlation between the quantitative viral loads measured by the in-house assay and the RealStar® Adenovirus PCR Kit ($ R^{2} $ = 0.984), with an average bias of − 0.16 $ log_{10} $ copies/mL. Conclusions The in-house adenovirus assay is a sensitive and reliable assay with lower cost for the detection and quantification of adenoviral DNA when compared to the RealStar® Adenovirus PCR Kit. Human adenovirus (dpeaa)DE-He213 Real-time PCR (dpeaa)DE-He213 RealStar® Adenovirus PCR Kit (dpeaa)DE-He213 Yip, Cyril C. Y. aut Sridhar, Siddharth aut Leung, Kit-Hang aut Cheng, Andrew K. W. aut Fung, Ami M. Y. aut Lam, Ho-Yin aut Chan, Kwok-Hung aut Chan, Jasper F. W. aut Cheng, Vincent C. C. aut Tang, Bone S. F. aut Yuen, Kwok-Yung aut Enthalten in Virology journal London : BioMed Central, 2004 15(2018), 1 vom: 27. Sept. (DE-627)394165004 (DE-600)2160640-7 1743-422X nnns volume:15 year:2018 number:1 day:27 month:09 https://dx.doi.org/10.1186/s12985-018-1059-7 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2018 1 27 09 |
allfields_unstemmed |
10.1186/s12985-018-1059-7 doi (DE-627)SPR029268079 (SPR)s12985-018-1059-7-e DE-627 ger DE-627 rakwb eng Wong, Samson S. Y. verfasserin aut Comparative evaluation of a laboratory-developed real-time PCR assay and RealStar® Adenovirus PCR Kit for quantitative detection of human adenovirus 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2018 Background Human adenoviruses are common causes of community-acquired respiratory tract and enteric infections. Severe disseminated infections with high mortality rates may be seen in immunocompromised individuals. An accurate and cost-effective quantitative assay is essential not only for laboratory diagnosis of adenoviral infections, but also for monitoring of response to antiviral treatment. The diagnostic performance of an in-house quantitative polymerase chain reaction assay was compared to a commercial system. Methods The analytical sensitivity, specificity, linearity, precision and accuracy of an in-house adenovirus quantitative polymerase chain reaction assay were evaluated against the RealStar® Adenovirus PCR Kit (Altona Diagnostics GmbH, Hamburg, Germany), using 122 clinical specimens and 18 proficiency testing samples. Results Linear regression analysis of the quantitative results by the in-house assay showed the dynamic range from 2.60 to 9 $ log_{10} $ (plasma) and 2.94 to 9 $ log_{10} $ (viral transport medium) copies/mL, with the coefficient of determination ($ R^{2} $) of 0.996 and 0.998, respectively. A dilution series demonstrated the limits of detection and lower limits of quantification for plasma were 2.06 $ log_{10} $ and 2.60 $ log_{10} $ copies/mL and those for viral transport medium were 2.31 $ log_{10} $ and 2.94 $ log_{10} $ copies/mL respectively. The precision of the in-house assay was highly reproducible among runs with coefficients of variance ranging from 0.07 to 3.21% for plasma and 0.17% to 2.11% for viral transport medium. A comparison of 52 matched samples showed an excellent correlation between the quantitative viral loads measured by the in-house assay and the RealStar® Adenovirus PCR Kit ($ R^{2} $ = 0.984), with an average bias of − 0.16 $ log_{10} $ copies/mL. Conclusions The in-house adenovirus assay is a sensitive and reliable assay with lower cost for the detection and quantification of adenoviral DNA when compared to the RealStar® Adenovirus PCR Kit. Human adenovirus (dpeaa)DE-He213 Real-time PCR (dpeaa)DE-He213 RealStar® Adenovirus PCR Kit (dpeaa)DE-He213 Yip, Cyril C. Y. aut Sridhar, Siddharth aut Leung, Kit-Hang aut Cheng, Andrew K. W. aut Fung, Ami M. Y. aut Lam, Ho-Yin aut Chan, Kwok-Hung aut Chan, Jasper F. W. aut Cheng, Vincent C. C. aut Tang, Bone S. F. aut Yuen, Kwok-Yung aut Enthalten in Virology journal London : BioMed Central, 2004 15(2018), 1 vom: 27. Sept. (DE-627)394165004 (DE-600)2160640-7 1743-422X nnns volume:15 year:2018 number:1 day:27 month:09 https://dx.doi.org/10.1186/s12985-018-1059-7 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2018 1 27 09 |
allfieldsGer |
10.1186/s12985-018-1059-7 doi (DE-627)SPR029268079 (SPR)s12985-018-1059-7-e DE-627 ger DE-627 rakwb eng Wong, Samson S. Y. verfasserin aut Comparative evaluation of a laboratory-developed real-time PCR assay and RealStar® Adenovirus PCR Kit for quantitative detection of human adenovirus 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2018 Background Human adenoviruses are common causes of community-acquired respiratory tract and enteric infections. Severe disseminated infections with high mortality rates may be seen in immunocompromised individuals. An accurate and cost-effective quantitative assay is essential not only for laboratory diagnosis of adenoviral infections, but also for monitoring of response to antiviral treatment. The diagnostic performance of an in-house quantitative polymerase chain reaction assay was compared to a commercial system. Methods The analytical sensitivity, specificity, linearity, precision and accuracy of an in-house adenovirus quantitative polymerase chain reaction assay were evaluated against the RealStar® Adenovirus PCR Kit (Altona Diagnostics GmbH, Hamburg, Germany), using 122 clinical specimens and 18 proficiency testing samples. Results Linear regression analysis of the quantitative results by the in-house assay showed the dynamic range from 2.60 to 9 $ log_{10} $ (plasma) and 2.94 to 9 $ log_{10} $ (viral transport medium) copies/mL, with the coefficient of determination ($ R^{2} $) of 0.996 and 0.998, respectively. A dilution series demonstrated the limits of detection and lower limits of quantification for plasma were 2.06 $ log_{10} $ and 2.60 $ log_{10} $ copies/mL and those for viral transport medium were 2.31 $ log_{10} $ and 2.94 $ log_{10} $ copies/mL respectively. The precision of the in-house assay was highly reproducible among runs with coefficients of variance ranging from 0.07 to 3.21% for plasma and 0.17% to 2.11% for viral transport medium. A comparison of 52 matched samples showed an excellent correlation between the quantitative viral loads measured by the in-house assay and the RealStar® Adenovirus PCR Kit ($ R^{2} $ = 0.984), with an average bias of − 0.16 $ log_{10} $ copies/mL. Conclusions The in-house adenovirus assay is a sensitive and reliable assay with lower cost for the detection and quantification of adenoviral DNA when compared to the RealStar® Adenovirus PCR Kit. Human adenovirus (dpeaa)DE-He213 Real-time PCR (dpeaa)DE-He213 RealStar® Adenovirus PCR Kit (dpeaa)DE-He213 Yip, Cyril C. Y. aut Sridhar, Siddharth aut Leung, Kit-Hang aut Cheng, Andrew K. W. aut Fung, Ami M. Y. aut Lam, Ho-Yin aut Chan, Kwok-Hung aut Chan, Jasper F. W. aut Cheng, Vincent C. C. aut Tang, Bone S. F. aut Yuen, Kwok-Yung aut Enthalten in Virology journal London : BioMed Central, 2004 15(2018), 1 vom: 27. Sept. (DE-627)394165004 (DE-600)2160640-7 1743-422X nnns volume:15 year:2018 number:1 day:27 month:09 https://dx.doi.org/10.1186/s12985-018-1059-7 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2018 1 27 09 |
allfieldsSound |
10.1186/s12985-018-1059-7 doi (DE-627)SPR029268079 (SPR)s12985-018-1059-7-e DE-627 ger DE-627 rakwb eng Wong, Samson S. Y. verfasserin aut Comparative evaluation of a laboratory-developed real-time PCR assay and RealStar® Adenovirus PCR Kit for quantitative detection of human adenovirus 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2018 Background Human adenoviruses are common causes of community-acquired respiratory tract and enteric infections. Severe disseminated infections with high mortality rates may be seen in immunocompromised individuals. An accurate and cost-effective quantitative assay is essential not only for laboratory diagnosis of adenoviral infections, but also for monitoring of response to antiviral treatment. The diagnostic performance of an in-house quantitative polymerase chain reaction assay was compared to a commercial system. Methods The analytical sensitivity, specificity, linearity, precision and accuracy of an in-house adenovirus quantitative polymerase chain reaction assay were evaluated against the RealStar® Adenovirus PCR Kit (Altona Diagnostics GmbH, Hamburg, Germany), using 122 clinical specimens and 18 proficiency testing samples. Results Linear regression analysis of the quantitative results by the in-house assay showed the dynamic range from 2.60 to 9 $ log_{10} $ (plasma) and 2.94 to 9 $ log_{10} $ (viral transport medium) copies/mL, with the coefficient of determination ($ R^{2} $) of 0.996 and 0.998, respectively. A dilution series demonstrated the limits of detection and lower limits of quantification for plasma were 2.06 $ log_{10} $ and 2.60 $ log_{10} $ copies/mL and those for viral transport medium were 2.31 $ log_{10} $ and 2.94 $ log_{10} $ copies/mL respectively. The precision of the in-house assay was highly reproducible among runs with coefficients of variance ranging from 0.07 to 3.21% for plasma and 0.17% to 2.11% for viral transport medium. A comparison of 52 matched samples showed an excellent correlation between the quantitative viral loads measured by the in-house assay and the RealStar® Adenovirus PCR Kit ($ R^{2} $ = 0.984), with an average bias of − 0.16 $ log_{10} $ copies/mL. Conclusions The in-house adenovirus assay is a sensitive and reliable assay with lower cost for the detection and quantification of adenoviral DNA when compared to the RealStar® Adenovirus PCR Kit. Human adenovirus (dpeaa)DE-He213 Real-time PCR (dpeaa)DE-He213 RealStar® Adenovirus PCR Kit (dpeaa)DE-He213 Yip, Cyril C. Y. aut Sridhar, Siddharth aut Leung, Kit-Hang aut Cheng, Andrew K. W. aut Fung, Ami M. Y. aut Lam, Ho-Yin aut Chan, Kwok-Hung aut Chan, Jasper F. W. aut Cheng, Vincent C. C. aut Tang, Bone S. F. aut Yuen, Kwok-Yung aut Enthalten in Virology journal London : BioMed Central, 2004 15(2018), 1 vom: 27. Sept. (DE-627)394165004 (DE-600)2160640-7 1743-422X nnns volume:15 year:2018 number:1 day:27 month:09 https://dx.doi.org/10.1186/s12985-018-1059-7 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2018 1 27 09 |
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Wong, Samson S. Y. @@aut@@ Yip, Cyril C. Y. @@aut@@ Sridhar, Siddharth @@aut@@ Leung, Kit-Hang @@aut@@ Cheng, Andrew K. W. @@aut@@ Fung, Ami M. Y. @@aut@@ Lam, Ho-Yin @@aut@@ Chan, Kwok-Hung @@aut@@ Chan, Jasper F. W. @@aut@@ Cheng, Vincent C. C. @@aut@@ Tang, Bone S. F. @@aut@@ Yuen, Kwok-Yung @@aut@@ |
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Severe disseminated infections with high mortality rates may be seen in immunocompromised individuals. An accurate and cost-effective quantitative assay is essential not only for laboratory diagnosis of adenoviral infections, but also for monitoring of response to antiviral treatment. The diagnostic performance of an in-house quantitative polymerase chain reaction assay was compared to a commercial system. Methods The analytical sensitivity, specificity, linearity, precision and accuracy of an in-house adenovirus quantitative polymerase chain reaction assay were evaluated against the RealStar® Adenovirus PCR Kit (Altona Diagnostics GmbH, Hamburg, Germany), using 122 clinical specimens and 18 proficiency testing samples. Results Linear regression analysis of the quantitative results by the in-house assay showed the dynamic range from 2.60 to 9 $ log_{10} $ (plasma) and 2.94 to 9 $ log_{10} $ (viral transport medium) copies/mL, with the coefficient of determination ($ R^{2} $) of 0.996 and 0.998, respectively. A dilution series demonstrated the limits of detection and lower limits of quantification for plasma were 2.06 $ log_{10} $ and 2.60 $ log_{10} $ copies/mL and those for viral transport medium were 2.31 $ log_{10} $ and 2.94 $ log_{10} $ copies/mL respectively. The precision of the in-house assay was highly reproducible among runs with coefficients of variance ranging from 0.07 to 3.21% for plasma and 0.17% to 2.11% for viral transport medium. A comparison of 52 matched samples showed an excellent correlation between the quantitative viral loads measured by the in-house assay and the RealStar® Adenovirus PCR Kit ($ R^{2} $ = 0.984), with an average bias of − 0.16 $ log_{10} $ copies/mL. 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Wong, Samson S. Y. |
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Wong, Samson S. Y. misc Human adenovirus misc Real-time PCR misc RealStar® Adenovirus PCR Kit Comparative evaluation of a laboratory-developed real-time PCR assay and RealStar® Adenovirus PCR Kit for quantitative detection of human adenovirus |
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Comparative evaluation of a laboratory-developed real-time PCR assay and RealStar® Adenovirus PCR Kit for quantitative detection of human adenovirus Human adenovirus (dpeaa)DE-He213 Real-time PCR (dpeaa)DE-He213 RealStar® Adenovirus PCR Kit (dpeaa)DE-He213 |
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Comparative evaluation of a laboratory-developed real-time PCR assay and RealStar® Adenovirus PCR Kit for quantitative detection of human adenovirus |
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(DE-627)SPR029268079 (SPR)s12985-018-1059-7-e |
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Comparative evaluation of a laboratory-developed real-time PCR assay and RealStar® Adenovirus PCR Kit for quantitative detection of human adenovirus |
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Wong, Samson S. Y. |
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Virology journal |
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Wong, Samson S. Y. Yip, Cyril C. Y. Sridhar, Siddharth Leung, Kit-Hang Cheng, Andrew K. W. Fung, Ami M. Y. Lam, Ho-Yin Chan, Kwok-Hung Chan, Jasper F. W. Cheng, Vincent C. C. Tang, Bone S. F. Yuen, Kwok-Yung |
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Elektronische Aufsätze |
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Wong, Samson S. Y. |
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10.1186/s12985-018-1059-7 |
title_sort |
comparative evaluation of a laboratory-developed real-time pcr assay and realstar® adenovirus pcr kit for quantitative detection of human adenovirus |
title_auth |
Comparative evaluation of a laboratory-developed real-time PCR assay and RealStar® Adenovirus PCR Kit for quantitative detection of human adenovirus |
abstract |
Background Human adenoviruses are common causes of community-acquired respiratory tract and enteric infections. Severe disseminated infections with high mortality rates may be seen in immunocompromised individuals. An accurate and cost-effective quantitative assay is essential not only for laboratory diagnosis of adenoviral infections, but also for monitoring of response to antiviral treatment. The diagnostic performance of an in-house quantitative polymerase chain reaction assay was compared to a commercial system. Methods The analytical sensitivity, specificity, linearity, precision and accuracy of an in-house adenovirus quantitative polymerase chain reaction assay were evaluated against the RealStar® Adenovirus PCR Kit (Altona Diagnostics GmbH, Hamburg, Germany), using 122 clinical specimens and 18 proficiency testing samples. Results Linear regression analysis of the quantitative results by the in-house assay showed the dynamic range from 2.60 to 9 $ log_{10} $ (plasma) and 2.94 to 9 $ log_{10} $ (viral transport medium) copies/mL, with the coefficient of determination ($ R^{2} $) of 0.996 and 0.998, respectively. A dilution series demonstrated the limits of detection and lower limits of quantification for plasma were 2.06 $ log_{10} $ and 2.60 $ log_{10} $ copies/mL and those for viral transport medium were 2.31 $ log_{10} $ and 2.94 $ log_{10} $ copies/mL respectively. The precision of the in-house assay was highly reproducible among runs with coefficients of variance ranging from 0.07 to 3.21% for plasma and 0.17% to 2.11% for viral transport medium. A comparison of 52 matched samples showed an excellent correlation between the quantitative viral loads measured by the in-house assay and the RealStar® Adenovirus PCR Kit ($ R^{2} $ = 0.984), with an average bias of − 0.16 $ log_{10} $ copies/mL. Conclusions The in-house adenovirus assay is a sensitive and reliable assay with lower cost for the detection and quantification of adenoviral DNA when compared to the RealStar® Adenovirus PCR Kit. © The Author(s). 2018 |
abstractGer |
Background Human adenoviruses are common causes of community-acquired respiratory tract and enteric infections. Severe disseminated infections with high mortality rates may be seen in immunocompromised individuals. An accurate and cost-effective quantitative assay is essential not only for laboratory diagnosis of adenoviral infections, but also for monitoring of response to antiviral treatment. The diagnostic performance of an in-house quantitative polymerase chain reaction assay was compared to a commercial system. Methods The analytical sensitivity, specificity, linearity, precision and accuracy of an in-house adenovirus quantitative polymerase chain reaction assay were evaluated against the RealStar® Adenovirus PCR Kit (Altona Diagnostics GmbH, Hamburg, Germany), using 122 clinical specimens and 18 proficiency testing samples. Results Linear regression analysis of the quantitative results by the in-house assay showed the dynamic range from 2.60 to 9 $ log_{10} $ (plasma) and 2.94 to 9 $ log_{10} $ (viral transport medium) copies/mL, with the coefficient of determination ($ R^{2} $) of 0.996 and 0.998, respectively. A dilution series demonstrated the limits of detection and lower limits of quantification for plasma were 2.06 $ log_{10} $ and 2.60 $ log_{10} $ copies/mL and those for viral transport medium were 2.31 $ log_{10} $ and 2.94 $ log_{10} $ copies/mL respectively. The precision of the in-house assay was highly reproducible among runs with coefficients of variance ranging from 0.07 to 3.21% for plasma and 0.17% to 2.11% for viral transport medium. A comparison of 52 matched samples showed an excellent correlation between the quantitative viral loads measured by the in-house assay and the RealStar® Adenovirus PCR Kit ($ R^{2} $ = 0.984), with an average bias of − 0.16 $ log_{10} $ copies/mL. Conclusions The in-house adenovirus assay is a sensitive and reliable assay with lower cost for the detection and quantification of adenoviral DNA when compared to the RealStar® Adenovirus PCR Kit. © The Author(s). 2018 |
abstract_unstemmed |
Background Human adenoviruses are common causes of community-acquired respiratory tract and enteric infections. Severe disseminated infections with high mortality rates may be seen in immunocompromised individuals. An accurate and cost-effective quantitative assay is essential not only for laboratory diagnosis of adenoviral infections, but also for monitoring of response to antiviral treatment. The diagnostic performance of an in-house quantitative polymerase chain reaction assay was compared to a commercial system. Methods The analytical sensitivity, specificity, linearity, precision and accuracy of an in-house adenovirus quantitative polymerase chain reaction assay were evaluated against the RealStar® Adenovirus PCR Kit (Altona Diagnostics GmbH, Hamburg, Germany), using 122 clinical specimens and 18 proficiency testing samples. Results Linear regression analysis of the quantitative results by the in-house assay showed the dynamic range from 2.60 to 9 $ log_{10} $ (plasma) and 2.94 to 9 $ log_{10} $ (viral transport medium) copies/mL, with the coefficient of determination ($ R^{2} $) of 0.996 and 0.998, respectively. A dilution series demonstrated the limits of detection and lower limits of quantification for plasma were 2.06 $ log_{10} $ and 2.60 $ log_{10} $ copies/mL and those for viral transport medium were 2.31 $ log_{10} $ and 2.94 $ log_{10} $ copies/mL respectively. The precision of the in-house assay was highly reproducible among runs with coefficients of variance ranging from 0.07 to 3.21% for plasma and 0.17% to 2.11% for viral transport medium. A comparison of 52 matched samples showed an excellent correlation between the quantitative viral loads measured by the in-house assay and the RealStar® Adenovirus PCR Kit ($ R^{2} $ = 0.984), with an average bias of − 0.16 $ log_{10} $ copies/mL. Conclusions The in-house adenovirus assay is a sensitive and reliable assay with lower cost for the detection and quantification of adenoviral DNA when compared to the RealStar® Adenovirus PCR Kit. © The Author(s). 2018 |
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title_short |
Comparative evaluation of a laboratory-developed real-time PCR assay and RealStar® Adenovirus PCR Kit for quantitative detection of human adenovirus |
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https://dx.doi.org/10.1186/s12985-018-1059-7 |
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Yip, Cyril C. Y. Sridhar, Siddharth Leung, Kit-Hang Cheng, Andrew K. W. Fung, Ami M. Y. Lam, Ho-Yin Chan, Kwok-Hung Chan, Jasper F. W. Cheng, Vincent C. C. Tang, Bone S. F. Yuen, Kwok-Yung |
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Yip, Cyril C. Y. Sridhar, Siddharth Leung, Kit-Hang Cheng, Andrew K. W. Fung, Ami M. Y. Lam, Ho-Yin Chan, Kwok-Hung Chan, Jasper F. W. Cheng, Vincent C. C. Tang, Bone S. F. Yuen, Kwok-Yung |
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