Comparative evaluation of a laboratory-developed real-time PCR assay and RealStar® Adenovirus PCR Kit for quantitative detection of human adenovirus

Background Human adenoviruses are common causes of community-acquired respiratory tract and enteric infections. Severe disseminated infections with high mortality rates may be seen in immunocompromised individuals. An accurate and cost-effective quantitative assay is essential not only for laboratory diagnosis of adenoviral infections, but also for monitoring of response to antiviral treatment. The diagnostic performance of an in-house quantitative polymerase chain reaction assay was compared to a commercial system. Methods The analytical sensitivity, specificity, linearity, precision and accuracy of an in-house adenovirus quantitative polymerase chain reaction assay were evaluated against the RealStar® Adenovirus PCR Kit (Altona Diagnostics GmbH, Hamburg, Germany), using 122 clinical specimens and 18 proficiency testing samples. Results Linear regression analysis of the quantitative results by the in-house assay showed the dynamic range from 2.60 to 9 $ log_{10} $ (plasma) and 2.94 to 9 $ log_{10} $ (viral transport medium) copies/mL, with the coefficient of determination ($ R^{2} $) of 0.996 and 0.998, respectively. A dilution series demonstrated the limits of detection and lower limits of quantification for plasma were 2.06 $ log_{10} $ and 2.60 $ log_{10} $ copies/mL and those for viral transport medium were 2.31 $ log_{10} $ and 2.94 $ log_{10} $ copies/mL respectively. The precision of the in-house assay was highly reproducible among runs with coefficients of variance ranging from 0.07 to 3.21% for plasma and 0.17% to 2.11% for viral transport medium. A comparison of 52 matched samples showed an excellent correlation between the quantitative viral loads measured by the in-house assay and the RealStar® Adenovirus PCR Kit ($ R^{2} $ = 0.984), with an average bias of − 0.16 $ log_{10} $ copies/mL. Conclusions The in-house adenovirus assay is a sensitive and reliable assay with lower cost for the detection and quantification of adenoviral DNA when compared to the RealStar® Adenovirus PCR Kit. Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Wong, Samson S. Y. [verfasserIn] Yip, Cyril C. Y. Sridhar, Siddharth Leung, Kit-Hang Cheng, Andrew K. W. Fung, Ami M. Y. Lam, Ho-Yin Chan, Kwok-Hung Chan, Jasper F. W. Cheng, Vincent C. C. Tang, Bone S. F. Yuen, Kwok-Yung

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2018

Schlagwörter:	Human adenovirus Real-time PCR RealStar® Adenovirus PCR Kit

Anmerkung:	© The Author(s). 2018

Übergeordnetes Werk:	Enthalten in: Virology journal - London : BioMed Central, 2004, 15(2018), 1 vom: 27. Sept.
Übergeordnetes Werk:	volume:15 ; year:2018 ; number:1 ; day:27 ; month:09

Links:	Volltext

DOI / URN:	10.1186/s12985-018-1059-7

Katalog-ID:	SPR029268079

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100	1		\|a Wong, Samson S. Y. \|e verfasserin \|4 aut
245	1	0	\|a Comparative evaluation of a laboratory-developed real-time PCR assay and RealStar® Adenovirus PCR Kit for quantitative detection of human adenovirus
264		1	\|c 2018
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337			\|a Computermedien \|b c \|2 rdamedia
338			\|a Online-Ressource \|b cr \|2 rdacarrier
500			\|a © The Author(s). 2018
520			\|a Background Human adenoviruses are common causes of community-acquired respiratory tract and enteric infections. Severe disseminated infections with high mortality rates may be seen in immunocompromised individuals. An accurate and cost-effective quantitative assay is essential not only for laboratory diagnosis of adenoviral infections, but also for monitoring of response to antiviral treatment. The diagnostic performance of an in-house quantitative polymerase chain reaction assay was compared to a commercial system. Methods The analytical sensitivity, specificity, linearity, precision and accuracy of an in-house adenovirus quantitative polymerase chain reaction assay were evaluated against the RealStar® Adenovirus PCR Kit (Altona Diagnostics GmbH, Hamburg, Germany), using 122 clinical specimens and 18 proficiency testing samples. Results Linear regression analysis of the quantitative results by the in-house assay showed the dynamic range from 2.60 to 9 $ log_{10} $ (plasma) and 2.94 to 9 $ log_{10} $ (viral transport medium) copies/mL, with the coefficient of determination ($ R^{2} $) of 0.996 and 0.998, respectively. A dilution series demonstrated the limits of detection and lower limits of quantification for plasma were 2.06 $ log_{10} $ and 2.60 $ log_{10} $ copies/mL and those for viral transport medium were 2.31 $ log_{10} $ and 2.94 $ log_{10} $ copies/mL respectively. The precision of the in-house assay was highly reproducible among runs with coefficients of variance ranging from 0.07 to 3.21% for plasma and 0.17% to 2.11% for viral transport medium. A comparison of 52 matched samples showed an excellent correlation between the quantitative viral loads measured by the in-house assay and the RealStar® Adenovirus PCR Kit ($ R^{2} $ = 0.984), with an average bias of − 0.16 $ log_{10} $ copies/mL. Conclusions The in-house adenovirus assay is a sensitive and reliable assay with lower cost for the detection and quantification of adenoviral DNA when compared to the RealStar® Adenovirus PCR Kit.
650		4	\|a Human adenovirus \|7 (dpeaa)DE-He213
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650		4	\|a RealStar® Adenovirus PCR Kit \|7 (dpeaa)DE-He213
700	1		\|a Yip, Cyril C. Y. \|4 aut
700	1		\|a Sridhar, Siddharth \|4 aut
700	1		\|a Leung, Kit-Hang \|4 aut
700	1		\|a Cheng, Andrew K. W. \|4 aut
700	1		\|a Fung, Ami M. Y. \|4 aut
700	1		\|a Lam, Ho-Yin \|4 aut
700	1		\|a Chan, Kwok-Hung \|4 aut
700	1		\|a Chan, Jasper F. W. \|4 aut
700	1		\|a Cheng, Vincent C. C. \|4 aut
700	1		\|a Tang, Bone S. F. \|4 aut
700	1		\|a Yuen, Kwok-Yung \|4 aut
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773	1	8	\|g volume:15 \|g year:2018 \|g number:1 \|g day:27 \|g month:09
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912			\|a GBV_ILN_24
912			\|a GBV_ILN_31
912			\|a GBV_ILN_39
912			\|a GBV_ILN_40
912			\|a GBV_ILN_60
912			\|a GBV_ILN_62
912			\|a GBV_ILN_63
912			\|a GBV_ILN_65
912			\|a GBV_ILN_69
912			\|a GBV_ILN_73
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912			\|a GBV_ILN_95
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912			\|a GBV_ILN_151
912			\|a GBV_ILN_161
912			\|a GBV_ILN_170
912			\|a GBV_ILN_206
912			\|a GBV_ILN_213
912			\|a GBV_ILN_230
912			\|a GBV_ILN_285
912			\|a GBV_ILN_293
912			\|a GBV_ILN_602
912			\|a GBV_ILN_702
912			\|a GBV_ILN_2001
912			\|a GBV_ILN_2003
912			\|a GBV_ILN_2005
912			\|a GBV_ILN_2006
912			\|a GBV_ILN_2008
912			\|a GBV_ILN_2009
912			\|a GBV_ILN_2010
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912			\|a GBV_ILN_2014
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912			\|a GBV_ILN_2061
912			\|a GBV_ILN_2111
912			\|a GBV_ILN_2113
912			\|a GBV_ILN_2190
912			\|a GBV_ILN_4012
912			\|a GBV_ILN_4037
912			\|a GBV_ILN_4112
912			\|a GBV_ILN_4125
912			\|a GBV_ILN_4126
912			\|a GBV_ILN_4249
912			\|a GBV_ILN_4305
912			\|a GBV_ILN_4306
912			\|a GBV_ILN_4307
912			\|a GBV_ILN_4313
912			\|a GBV_ILN_4322
912			\|a GBV_ILN_4323
912			\|a GBV_ILN_4324
912			\|a GBV_ILN_4325
912			\|a GBV_ILN_4338
912			\|a GBV_ILN_4367
912			\|a GBV_ILN_4700
951			\|a AR
952			\|d 15 \|j 2018 \|e 1 \|b 27 \|c 09

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allfields	10.1186/s12985-018-1059-7 doi (DE-627)SPR029268079 (SPR)s12985-018-1059-7-e DE-627 ger DE-627 rakwb eng Wong, Samson S. Y. verfasserin aut Comparative evaluation of a laboratory-developed real-time PCR assay and RealStar® Adenovirus PCR Kit for quantitative detection of human adenovirus 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2018 Background Human adenoviruses are common causes of community-acquired respiratory tract and enteric infections. Severe disseminated infections with high mortality rates may be seen in immunocompromised individuals. An accurate and cost-effective quantitative assay is essential not only for laboratory diagnosis of adenoviral infections, but also for monitoring of response to antiviral treatment. The diagnostic performance of an in-house quantitative polymerase chain reaction assay was compared to a commercial system. Methods The analytical sensitivity, specificity, linearity, precision and accuracy of an in-house adenovirus quantitative polymerase chain reaction assay were evaluated against the RealStar® Adenovirus PCR Kit (Altona Diagnostics GmbH, Hamburg, Germany), using 122 clinical specimens and 18 proficiency testing samples. Results Linear regression analysis of the quantitative results by the in-house assay showed the dynamic range from 2.60 to 9 $ log_{10} $ (plasma) and 2.94 to 9 $ log_{10} $ (viral transport medium) copies/mL, with the coefficient of determination ($ R^{2} $) of 0.996 and 0.998, respectively. A dilution series demonstrated the limits of detection and lower limits of quantification for plasma were 2.06 $ log_{10} $ and 2.60 $ log_{10} $ copies/mL and those for viral transport medium were 2.31 $ log_{10} $ and 2.94 $ log_{10} $ copies/mL respectively. The precision of the in-house assay was highly reproducible among runs with coefficients of variance ranging from 0.07 to 3.21% for plasma and 0.17% to 2.11% for viral transport medium. A comparison of 52 matched samples showed an excellent correlation between the quantitative viral loads measured by the in-house assay and the RealStar® Adenovirus PCR Kit ($ R^{2} $ = 0.984), with an average bias of − 0.16 $ log_{10} $ copies/mL. Conclusions The in-house adenovirus assay is a sensitive and reliable assay with lower cost for the detection and quantification of adenoviral DNA when compared to the RealStar® Adenovirus PCR Kit. Human adenovirus (dpeaa)DE-He213 Real-time PCR (dpeaa)DE-He213 RealStar® Adenovirus PCR Kit (dpeaa)DE-He213 Yip, Cyril C. Y. aut Sridhar, Siddharth aut Leung, Kit-Hang aut Cheng, Andrew K. W. aut Fung, Ami M. Y. aut Lam, Ho-Yin aut Chan, Kwok-Hung aut Chan, Jasper F. W. aut Cheng, Vincent C. C. aut Tang, Bone S. F. aut Yuen, Kwok-Yung aut Enthalten in Virology journal London : BioMed Central, 2004 15(2018), 1 vom: 27. Sept. (DE-627)394165004 (DE-600)2160640-7 1743-422X nnns volume:15 year:2018 number:1 day:27 month:09 https://dx.doi.org/10.1186/s12985-018-1059-7 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2018 1 27 09
spelling	10.1186/s12985-018-1059-7 doi (DE-627)SPR029268079 (SPR)s12985-018-1059-7-e DE-627 ger DE-627 rakwb eng Wong, Samson S. Y. verfasserin aut Comparative evaluation of a laboratory-developed real-time PCR assay and RealStar® Adenovirus PCR Kit for quantitative detection of human adenovirus 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2018 Background Human adenoviruses are common causes of community-acquired respiratory tract and enteric infections. Severe disseminated infections with high mortality rates may be seen in immunocompromised individuals. An accurate and cost-effective quantitative assay is essential not only for laboratory diagnosis of adenoviral infections, but also for monitoring of response to antiviral treatment. The diagnostic performance of an in-house quantitative polymerase chain reaction assay was compared to a commercial system. Methods The analytical sensitivity, specificity, linearity, precision and accuracy of an in-house adenovirus quantitative polymerase chain reaction assay were evaluated against the RealStar® Adenovirus PCR Kit (Altona Diagnostics GmbH, Hamburg, Germany), using 122 clinical specimens and 18 proficiency testing samples. Results Linear regression analysis of the quantitative results by the in-house assay showed the dynamic range from 2.60 to 9 $ log_{10} $ (plasma) and 2.94 to 9 $ log_{10} $ (viral transport medium) copies/mL, with the coefficient of determination ($ R^{2} $) of 0.996 and 0.998, respectively. A dilution series demonstrated the limits of detection and lower limits of quantification for plasma were 2.06 $ log_{10} $ and 2.60 $ log_{10} $ copies/mL and those for viral transport medium were 2.31 $ log_{10} $ and 2.94 $ log_{10} $ copies/mL respectively. The precision of the in-house assay was highly reproducible among runs with coefficients of variance ranging from 0.07 to 3.21% for plasma and 0.17% to 2.11% for viral transport medium. A comparison of 52 matched samples showed an excellent correlation between the quantitative viral loads measured by the in-house assay and the RealStar® Adenovirus PCR Kit ($ R^{2} $ = 0.984), with an average bias of − 0.16 $ log_{10} $ copies/mL. Conclusions The in-house adenovirus assay is a sensitive and reliable assay with lower cost for the detection and quantification of adenoviral DNA when compared to the RealStar® Adenovirus PCR Kit. Human adenovirus (dpeaa)DE-He213 Real-time PCR (dpeaa)DE-He213 RealStar® Adenovirus PCR Kit (dpeaa)DE-He213 Yip, Cyril C. Y. aut Sridhar, Siddharth aut Leung, Kit-Hang aut Cheng, Andrew K. W. aut Fung, Ami M. Y. aut Lam, Ho-Yin aut Chan, Kwok-Hung aut Chan, Jasper F. W. aut Cheng, Vincent C. C. aut Tang, Bone S. F. aut Yuen, Kwok-Yung aut Enthalten in Virology journal London : BioMed Central, 2004 15(2018), 1 vom: 27. Sept. (DE-627)394165004 (DE-600)2160640-7 1743-422X nnns volume:15 year:2018 number:1 day:27 month:09 https://dx.doi.org/10.1186/s12985-018-1059-7 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2018 1 27 09
allfields_unstemmed	10.1186/s12985-018-1059-7 doi (DE-627)SPR029268079 (SPR)s12985-018-1059-7-e DE-627 ger DE-627 rakwb eng Wong, Samson S. Y. verfasserin aut Comparative evaluation of a laboratory-developed real-time PCR assay and RealStar® Adenovirus PCR Kit for quantitative detection of human adenovirus 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2018 Background Human adenoviruses are common causes of community-acquired respiratory tract and enteric infections. Severe disseminated infections with high mortality rates may be seen in immunocompromised individuals. An accurate and cost-effective quantitative assay is essential not only for laboratory diagnosis of adenoviral infections, but also for monitoring of response to antiviral treatment. The diagnostic performance of an in-house quantitative polymerase chain reaction assay was compared to a commercial system. Methods The analytical sensitivity, specificity, linearity, precision and accuracy of an in-house adenovirus quantitative polymerase chain reaction assay were evaluated against the RealStar® Adenovirus PCR Kit (Altona Diagnostics GmbH, Hamburg, Germany), using 122 clinical specimens and 18 proficiency testing samples. Results Linear regression analysis of the quantitative results by the in-house assay showed the dynamic range from 2.60 to 9 $ log_{10} $ (plasma) and 2.94 to 9 $ log_{10} $ (viral transport medium) copies/mL, with the coefficient of determination ($ R^{2} $) of 0.996 and 0.998, respectively. A dilution series demonstrated the limits of detection and lower limits of quantification for plasma were 2.06 $ log_{10} $ and 2.60 $ log_{10} $ copies/mL and those for viral transport medium were 2.31 $ log_{10} $ and 2.94 $ log_{10} $ copies/mL respectively. The precision of the in-house assay was highly reproducible among runs with coefficients of variance ranging from 0.07 to 3.21% for plasma and 0.17% to 2.11% for viral transport medium. A comparison of 52 matched samples showed an excellent correlation between the quantitative viral loads measured by the in-house assay and the RealStar® Adenovirus PCR Kit ($ R^{2} $ = 0.984), with an average bias of − 0.16 $ log_{10} $ copies/mL. Conclusions The in-house adenovirus assay is a sensitive and reliable assay with lower cost for the detection and quantification of adenoviral DNA when compared to the RealStar® Adenovirus PCR Kit. Human adenovirus (dpeaa)DE-He213 Real-time PCR (dpeaa)DE-He213 RealStar® Adenovirus PCR Kit (dpeaa)DE-He213 Yip, Cyril C. Y. aut Sridhar, Siddharth aut Leung, Kit-Hang aut Cheng, Andrew K. W. aut Fung, Ami M. Y. aut Lam, Ho-Yin aut Chan, Kwok-Hung aut Chan, Jasper F. W. aut Cheng, Vincent C. C. aut Tang, Bone S. F. aut Yuen, Kwok-Yung aut Enthalten in Virology journal London : BioMed Central, 2004 15(2018), 1 vom: 27. Sept. (DE-627)394165004 (DE-600)2160640-7 1743-422X nnns volume:15 year:2018 number:1 day:27 month:09 https://dx.doi.org/10.1186/s12985-018-1059-7 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2018 1 27 09
allfieldsGer	10.1186/s12985-018-1059-7 doi (DE-627)SPR029268079 (SPR)s12985-018-1059-7-e DE-627 ger DE-627 rakwb eng Wong, Samson S. Y. verfasserin aut Comparative evaluation of a laboratory-developed real-time PCR assay and RealStar® Adenovirus PCR Kit for quantitative detection of human adenovirus 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2018 Background Human adenoviruses are common causes of community-acquired respiratory tract and enteric infections. Severe disseminated infections with high mortality rates may be seen in immunocompromised individuals. An accurate and cost-effective quantitative assay is essential not only for laboratory diagnosis of adenoviral infections, but also for monitoring of response to antiviral treatment. The diagnostic performance of an in-house quantitative polymerase chain reaction assay was compared to a commercial system. Methods The analytical sensitivity, specificity, linearity, precision and accuracy of an in-house adenovirus quantitative polymerase chain reaction assay were evaluated against the RealStar® Adenovirus PCR Kit (Altona Diagnostics GmbH, Hamburg, Germany), using 122 clinical specimens and 18 proficiency testing samples. Results Linear regression analysis of the quantitative results by the in-house assay showed the dynamic range from 2.60 to 9 $ log_{10} $ (plasma) and 2.94 to 9 $ log_{10} $ (viral transport medium) copies/mL, with the coefficient of determination ($ R^{2} $) of 0.996 and 0.998, respectively. A dilution series demonstrated the limits of detection and lower limits of quantification for plasma were 2.06 $ log_{10} $ and 2.60 $ log_{10} $ copies/mL and those for viral transport medium were 2.31 $ log_{10} $ and 2.94 $ log_{10} $ copies/mL respectively. The precision of the in-house assay was highly reproducible among runs with coefficients of variance ranging from 0.07 to 3.21% for plasma and 0.17% to 2.11% for viral transport medium. A comparison of 52 matched samples showed an excellent correlation between the quantitative viral loads measured by the in-house assay and the RealStar® Adenovirus PCR Kit ($ R^{2} $ = 0.984), with an average bias of − 0.16 $ log_{10} $ copies/mL. Conclusions The in-house adenovirus assay is a sensitive and reliable assay with lower cost for the detection and quantification of adenoviral DNA when compared to the RealStar® Adenovirus PCR Kit. Human adenovirus (dpeaa)DE-He213 Real-time PCR (dpeaa)DE-He213 RealStar® Adenovirus PCR Kit (dpeaa)DE-He213 Yip, Cyril C. Y. aut Sridhar, Siddharth aut Leung, Kit-Hang aut Cheng, Andrew K. W. aut Fung, Ami M. Y. aut Lam, Ho-Yin aut Chan, Kwok-Hung aut Chan, Jasper F. W. aut Cheng, Vincent C. C. aut Tang, Bone S. F. aut Yuen, Kwok-Yung aut Enthalten in Virology journal London : BioMed Central, 2004 15(2018), 1 vom: 27. Sept. (DE-627)394165004 (DE-600)2160640-7 1743-422X nnns volume:15 year:2018 number:1 day:27 month:09 https://dx.doi.org/10.1186/s12985-018-1059-7 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2018 1 27 09
allfieldsSound	10.1186/s12985-018-1059-7 doi (DE-627)SPR029268079 (SPR)s12985-018-1059-7-e DE-627 ger DE-627 rakwb eng Wong, Samson S. Y. verfasserin aut Comparative evaluation of a laboratory-developed real-time PCR assay and RealStar® Adenovirus PCR Kit for quantitative detection of human adenovirus 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2018 Background Human adenoviruses are common causes of community-acquired respiratory tract and enteric infections. Severe disseminated infections with high mortality rates may be seen in immunocompromised individuals. An accurate and cost-effective quantitative assay is essential not only for laboratory diagnosis of adenoviral infections, but also for monitoring of response to antiviral treatment. The diagnostic performance of an in-house quantitative polymerase chain reaction assay was compared to a commercial system. Methods The analytical sensitivity, specificity, linearity, precision and accuracy of an in-house adenovirus quantitative polymerase chain reaction assay were evaluated against the RealStar® Adenovirus PCR Kit (Altona Diagnostics GmbH, Hamburg, Germany), using 122 clinical specimens and 18 proficiency testing samples. Results Linear regression analysis of the quantitative results by the in-house assay showed the dynamic range from 2.60 to 9 $ log_{10} $ (plasma) and 2.94 to 9 $ log_{10} $ (viral transport medium) copies/mL, with the coefficient of determination ($ R^{2} $) of 0.996 and 0.998, respectively. A dilution series demonstrated the limits of detection and lower limits of quantification for plasma were 2.06 $ log_{10} $ and 2.60 $ log_{10} $ copies/mL and those for viral transport medium were 2.31 $ log_{10} $ and 2.94 $ log_{10} $ copies/mL respectively. The precision of the in-house assay was highly reproducible among runs with coefficients of variance ranging from 0.07 to 3.21% for plasma and 0.17% to 2.11% for viral transport medium. A comparison of 52 matched samples showed an excellent correlation between the quantitative viral loads measured by the in-house assay and the RealStar® Adenovirus PCR Kit ($ R^{2} $ = 0.984), with an average bias of − 0.16 $ log_{10} $ copies/mL. Conclusions The in-house adenovirus assay is a sensitive and reliable assay with lower cost for the detection and quantification of adenoviral DNA when compared to the RealStar® Adenovirus PCR Kit. Human adenovirus (dpeaa)DE-He213 Real-time PCR (dpeaa)DE-He213 RealStar® Adenovirus PCR Kit (dpeaa)DE-He213 Yip, Cyril C. Y. aut Sridhar, Siddharth aut Leung, Kit-Hang aut Cheng, Andrew K. W. aut Fung, Ami M. Y. aut Lam, Ho-Yin aut Chan, Kwok-Hung aut Chan, Jasper F. W. aut Cheng, Vincent C. C. aut Tang, Bone S. F. aut Yuen, Kwok-Yung aut Enthalten in Virology journal London : BioMed Central, 2004 15(2018), 1 vom: 27. Sept. (DE-627)394165004 (DE-600)2160640-7 1743-422X nnns volume:15 year:2018 number:1 day:27 month:09 https://dx.doi.org/10.1186/s12985-018-1059-7 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2018 1 27 09
language	English
source	Enthalten in Virology journal 15(2018), 1 vom: 27. Sept. volume:15 year:2018 number:1 day:27 month:09
sourceStr	Enthalten in Virology journal 15(2018), 1 vom: 27. Sept. volume:15 year:2018 number:1 day:27 month:09
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Human adenovirus Real-time PCR RealStar® Adenovirus PCR Kit
isfreeaccess_bool	true
container_title	Virology journal
authorswithroles_txt_mv	Wong, Samson S. Y. @@aut@@ Yip, Cyril C. Y. @@aut@@ Sridhar, Siddharth @@aut@@ Leung, Kit-Hang @@aut@@ Cheng, Andrew K. W. @@aut@@ Fung, Ami M. Y. @@aut@@ Lam, Ho-Yin @@aut@@ Chan, Kwok-Hung @@aut@@ Chan, Jasper F. W. @@aut@@ Cheng, Vincent C. C. @@aut@@ Tang, Bone S. F. @@aut@@ Yuen, Kwok-Yung @@aut@@
publishDateDaySort_date	2018-09-27T00:00:00Z
hierarchy_top_id	394165004
id	SPR029268079
language_de	englisch
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Severe disseminated infections with high mortality rates may be seen in immunocompromised individuals. An accurate and cost-effective quantitative assay is essential not only for laboratory diagnosis of adenoviral infections, but also for monitoring of response to antiviral treatment. The diagnostic performance of an in-house quantitative polymerase chain reaction assay was compared to a commercial system. Methods The analytical sensitivity, specificity, linearity, precision and accuracy of an in-house adenovirus quantitative polymerase chain reaction assay were evaluated against the RealStar® Adenovirus PCR Kit (Altona Diagnostics GmbH, Hamburg, Germany), using 122 clinical specimens and 18 proficiency testing samples. Results Linear regression analysis of the quantitative results by the in-house assay showed the dynamic range from 2.60 to 9 $ log_{10} $ (plasma) and 2.94 to 9 $ log_{10} $ (viral transport medium) copies/mL, with the coefficient of determination ($ R^{2} $) of 0.996 and 0.998, respectively. A dilution series demonstrated the limits of detection and lower limits of quantification for plasma were 2.06 $ log_{10} $ and 2.60 $ log_{10} $ copies/mL and those for viral transport medium were 2.31 $ log_{10} $ and 2.94 $ log_{10} $ copies/mL respectively. The precision of the in-house assay was highly reproducible among runs with coefficients of variance ranging from 0.07 to 3.21% for plasma and 0.17% to 2.11% for viral transport medium. A comparison of 52 matched samples showed an excellent correlation between the quantitative viral loads measured by the in-house assay and the RealStar® Adenovirus PCR Kit ($ R^{2} $ = 0.984), with an average bias of − 0.16 $ log_{10} $ copies/mL. 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author	Wong, Samson S. Y.
spellingShingle	Wong, Samson S. Y. misc Human adenovirus misc Real-time PCR misc RealStar® Adenovirus PCR Kit Comparative evaluation of a laboratory-developed real-time PCR assay and RealStar® Adenovirus PCR Kit for quantitative detection of human adenovirus
authorStr	Wong, Samson S. Y.
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topic_title	Comparative evaluation of a laboratory-developed real-time PCR assay and RealStar® Adenovirus PCR Kit for quantitative detection of human adenovirus Human adenovirus (dpeaa)DE-He213 Real-time PCR (dpeaa)DE-He213 RealStar® Adenovirus PCR Kit (dpeaa)DE-He213
topic	misc Human adenovirus misc Real-time PCR misc RealStar® Adenovirus PCR Kit
topic_unstemmed	misc Human adenovirus misc Real-time PCR misc RealStar® Adenovirus PCR Kit
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title	Comparative evaluation of a laboratory-developed real-time PCR assay and RealStar® Adenovirus PCR Kit for quantitative detection of human adenovirus
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title_full	Comparative evaluation of a laboratory-developed real-time PCR assay and RealStar® Adenovirus PCR Kit for quantitative detection of human adenovirus
author_sort	Wong, Samson S. Y.
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author_browse	Wong, Samson S. Y. Yip, Cyril C. Y. Sridhar, Siddharth Leung, Kit-Hang Cheng, Andrew K. W. Fung, Ami M. Y. Lam, Ho-Yin Chan, Kwok-Hung Chan, Jasper F. W. Cheng, Vincent C. C. Tang, Bone S. F. Yuen, Kwok-Yung
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author-letter	Wong, Samson S. Y.
doi_str_mv	10.1186/s12985-018-1059-7
title_sort	comparative evaluation of a laboratory-developed real-time pcr assay and realstar® adenovirus pcr kit for quantitative detection of human adenovirus
title_auth	Comparative evaluation of a laboratory-developed real-time PCR assay and RealStar® Adenovirus PCR Kit for quantitative detection of human adenovirus
abstract	Background Human adenoviruses are common causes of community-acquired respiratory tract and enteric infections. Severe disseminated infections with high mortality rates may be seen in immunocompromised individuals. An accurate and cost-effective quantitative assay is essential not only for laboratory diagnosis of adenoviral infections, but also for monitoring of response to antiviral treatment. The diagnostic performance of an in-house quantitative polymerase chain reaction assay was compared to a commercial system. Methods The analytical sensitivity, specificity, linearity, precision and accuracy of an in-house adenovirus quantitative polymerase chain reaction assay were evaluated against the RealStar® Adenovirus PCR Kit (Altona Diagnostics GmbH, Hamburg, Germany), using 122 clinical specimens and 18 proficiency testing samples. Results Linear regression analysis of the quantitative results by the in-house assay showed the dynamic range from 2.60 to 9 $ log_{10} $ (plasma) and 2.94 to 9 $ log_{10} $ (viral transport medium) copies/mL, with the coefficient of determination ($ R^{2} $) of 0.996 and 0.998, respectively. A dilution series demonstrated the limits of detection and lower limits of quantification for plasma were 2.06 $ log_{10} $ and 2.60 $ log_{10} $ copies/mL and those for viral transport medium were 2.31 $ log_{10} $ and 2.94 $ log_{10} $ copies/mL respectively. The precision of the in-house assay was highly reproducible among runs with coefficients of variance ranging from 0.07 to 3.21% for plasma and 0.17% to 2.11% for viral transport medium. A comparison of 52 matched samples showed an excellent correlation between the quantitative viral loads measured by the in-house assay and the RealStar® Adenovirus PCR Kit ($ R^{2} $ = 0.984), with an average bias of − 0.16 $ log_{10} $ copies/mL. Conclusions The in-house adenovirus assay is a sensitive and reliable assay with lower cost for the detection and quantification of adenoviral DNA when compared to the RealStar® Adenovirus PCR Kit. © The Author(s). 2018
abstractGer	Background Human adenoviruses are common causes of community-acquired respiratory tract and enteric infections. Severe disseminated infections with high mortality rates may be seen in immunocompromised individuals. An accurate and cost-effective quantitative assay is essential not only for laboratory diagnosis of adenoviral infections, but also for monitoring of response to antiviral treatment. The diagnostic performance of an in-house quantitative polymerase chain reaction assay was compared to a commercial system. Methods The analytical sensitivity, specificity, linearity, precision and accuracy of an in-house adenovirus quantitative polymerase chain reaction assay were evaluated against the RealStar® Adenovirus PCR Kit (Altona Diagnostics GmbH, Hamburg, Germany), using 122 clinical specimens and 18 proficiency testing samples. Results Linear regression analysis of the quantitative results by the in-house assay showed the dynamic range from 2.60 to 9 $ log_{10} $ (plasma) and 2.94 to 9 $ log_{10} $ (viral transport medium) copies/mL, with the coefficient of determination ($ R^{2} $) of 0.996 and 0.998, respectively. A dilution series demonstrated the limits of detection and lower limits of quantification for plasma were 2.06 $ log_{10} $ and 2.60 $ log_{10} $ copies/mL and those for viral transport medium were 2.31 $ log_{10} $ and 2.94 $ log_{10} $ copies/mL respectively. The precision of the in-house assay was highly reproducible among runs with coefficients of variance ranging from 0.07 to 3.21% for plasma and 0.17% to 2.11% for viral transport medium. A comparison of 52 matched samples showed an excellent correlation between the quantitative viral loads measured by the in-house assay and the RealStar® Adenovirus PCR Kit ($ R^{2} $ = 0.984), with an average bias of − 0.16 $ log_{10} $ copies/mL. Conclusions The in-house adenovirus assay is a sensitive and reliable assay with lower cost for the detection and quantification of adenoviral DNA when compared to the RealStar® Adenovirus PCR Kit. © The Author(s). 2018
abstract_unstemmed	Background Human adenoviruses are common causes of community-acquired respiratory tract and enteric infections. Severe disseminated infections with high mortality rates may be seen in immunocompromised individuals. An accurate and cost-effective quantitative assay is essential not only for laboratory diagnosis of adenoviral infections, but also for monitoring of response to antiviral treatment. The diagnostic performance of an in-house quantitative polymerase chain reaction assay was compared to a commercial system. Methods The analytical sensitivity, specificity, linearity, precision and accuracy of an in-house adenovirus quantitative polymerase chain reaction assay were evaluated against the RealStar® Adenovirus PCR Kit (Altona Diagnostics GmbH, Hamburg, Germany), using 122 clinical specimens and 18 proficiency testing samples. Results Linear regression analysis of the quantitative results by the in-house assay showed the dynamic range from 2.60 to 9 $ log_{10} $ (plasma) and 2.94 to 9 $ log_{10} $ (viral transport medium) copies/mL, with the coefficient of determination ($ R^{2} $) of 0.996 and 0.998, respectively. A dilution series demonstrated the limits of detection and lower limits of quantification for plasma were 2.06 $ log_{10} $ and 2.60 $ log_{10} $ copies/mL and those for viral transport medium were 2.31 $ log_{10} $ and 2.94 $ log_{10} $ copies/mL respectively. The precision of the in-house assay was highly reproducible among runs with coefficients of variance ranging from 0.07 to 3.21% for plasma and 0.17% to 2.11% for viral transport medium. A comparison of 52 matched samples showed an excellent correlation between the quantitative viral loads measured by the in-house assay and the RealStar® Adenovirus PCR Kit ($ R^{2} $ = 0.984), with an average bias of − 0.16 $ log_{10} $ copies/mL. Conclusions The in-house adenovirus assay is a sensitive and reliable assay with lower cost for the detection and quantification of adenoviral DNA when compared to the RealStar® Adenovirus PCR Kit. © The Author(s). 2018
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title_short	Comparative evaluation of a laboratory-developed real-time PCR assay and RealStar® Adenovirus PCR Kit for quantitative detection of human adenovirus
url	https://dx.doi.org/10.1186/s12985-018-1059-7
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author2	Yip, Cyril C. Y. Sridhar, Siddharth Leung, Kit-Hang Cheng, Andrew K. W. Fung, Ami M. Y. Lam, Ho-Yin Chan, Kwok-Hung Chan, Jasper F. W. Cheng, Vincent C. C. Tang, Bone S. F. Yuen, Kwok-Yung
author2Str	Yip, Cyril C. Y. Sridhar, Siddharth Leung, Kit-Hang Cheng, Andrew K. W. Fung, Ami M. Y. Lam, Ho-Yin Chan, Kwok-Hung Chan, Jasper F. W. Cheng, Vincent C. C. Tang, Bone S. F. Yuen, Kwok-Yung
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Severe disseminated infections with high mortality rates may be seen in immunocompromised individuals. An accurate and cost-effective quantitative assay is essential not only for laboratory diagnosis of adenoviral infections, but also for monitoring of response to antiviral treatment. The diagnostic performance of an in-house quantitative polymerase chain reaction assay was compared to a commercial system. Methods The analytical sensitivity, specificity, linearity, precision and accuracy of an in-house adenovirus quantitative polymerase chain reaction assay were evaluated against the RealStar® Adenovirus PCR Kit (Altona Diagnostics GmbH, Hamburg, Germany), using 122 clinical specimens and 18 proficiency testing samples. 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Comparative evaluation of a laboratory-developed real-time PCR assay and RealStar® Adenovirus PCR Kit for quantitative detection of human adenovirus

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