The endo-lysosomal system of bEnd.3 and hCMEC/D3 brain endothelial cells
Background Brain endothelial cell-based in vitro models are among the most versatile tools in blood–brain barrier research for testing drug penetration to the central nervous system. Transcytosis of large pharmaceuticals across the brain capillary endothelium involves the complex endo-lysosomal syst...
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Gespeichert in:
| Autor*in: |
Toth, Andrea E. [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2019 |
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| Schlagwörter: |
bEnd.3 mouse brain endothelial cell line hCMEC/D3 human brain endothelial cell line |
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| Anmerkung: |
© The Author(s) 2019 |
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| Übergeordnetes Werk: |
Enthalten in: Cerebrospinal fluid research - London : BioMed Central, 2004, 16(2019), 1 vom: 30. Mai |
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| Übergeordnetes Werk: |
volume:16 ; year:2019 ; number:1 ; day:30 ; month:05 |
| Links: |
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| DOI / URN: |
10.1186/s12987-019-0134-9 |
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SPR029293324 |
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| 520 | |a Background Brain endothelial cell-based in vitro models are among the most versatile tools in blood–brain barrier research for testing drug penetration to the central nervous system. Transcytosis of large pharmaceuticals across the brain capillary endothelium involves the complex endo-lysosomal system. This system consists of several types of vesicle, such as early, late and recycling endosomes, retromer-positive structures, and lysosomes. Since the endo-lysosomal system in endothelial cell lines of in vitro blood–brain barrier models has not been investigated in detail, our aim was to characterize this system in different models. Methods For the investigation, we have chosen two widely-used models for in vitro drug transport studies: the bEnd.3 mouse and the hCMEC/D3 human brain endothelial cell line. We compared the structures and attributes of their endo-lysosomal system to that of primary porcine brain endothelial cells. Results We detected significant differences in the vesicular network regarding number, morphology, subcellular distribution and lysosomal activity. The retromer-positive vesicles of the primary cells were distinct in many ways from those of the cell lines. However, the cell lines showed higher lysosomal degradation activity than the primary cells. Additionally, the hCMEC/D3 possessed a strikingly unique ratio of recycling endosomes to late endosomes. Conclusions Taken together our data identify differences in the trafficking network of brain endothelial cells, essentially mapping the endo-lysosomal system of in vitro blood–brain barrier models. This knowledge is valuable for planning the optimal route across the blood–brain barrier and advancing drug delivery to the brain. | ||
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| 650 | 4 | |a hCMEC/D3 human brain endothelial cell line |7 (dpeaa)DE-He213 | |
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| 700 | 1 | |a Tomaka, Weronika |4 aut | |
| 700 | 1 | |a Abbott, N. Joan |4 aut | |
| 700 | 1 | |a Nielsen, Morten S. |4 aut | |
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10.1186/s12987-019-0134-9 doi (DE-627)SPR029293324 (SPR)s12987-019-0134-9-e DE-627 ger DE-627 rakwb eng Toth, Andrea E. verfasserin (orcid)0000-0001-6122-919X aut The endo-lysosomal system of bEnd.3 and hCMEC/D3 brain endothelial cells 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2019 Background Brain endothelial cell-based in vitro models are among the most versatile tools in blood–brain barrier research for testing drug penetration to the central nervous system. Transcytosis of large pharmaceuticals across the brain capillary endothelium involves the complex endo-lysosomal system. This system consists of several types of vesicle, such as early, late and recycling endosomes, retromer-positive structures, and lysosomes. Since the endo-lysosomal system in endothelial cell lines of in vitro blood–brain barrier models has not been investigated in detail, our aim was to characterize this system in different models. Methods For the investigation, we have chosen two widely-used models for in vitro drug transport studies: the bEnd.3 mouse and the hCMEC/D3 human brain endothelial cell line. We compared the structures and attributes of their endo-lysosomal system to that of primary porcine brain endothelial cells. Results We detected significant differences in the vesicular network regarding number, morphology, subcellular distribution and lysosomal activity. The retromer-positive vesicles of the primary cells were distinct in many ways from those of the cell lines. However, the cell lines showed higher lysosomal degradation activity than the primary cells. Additionally, the hCMEC/D3 possessed a strikingly unique ratio of recycling endosomes to late endosomes. Conclusions Taken together our data identify differences in the trafficking network of brain endothelial cells, essentially mapping the endo-lysosomal system of in vitro blood–brain barrier models. This knowledge is valuable for planning the optimal route across the blood–brain barrier and advancing drug delivery to the brain. bEnd.3 mouse brain endothelial cell line (dpeaa)DE-He213 hCMEC/D3 human brain endothelial cell line (dpeaa)DE-He213 Primary porcine brain endothelial cell (dpeaa)DE-He213 Blood–brain barrier (dpeaa)DE-He213 Endo-lysosomal system (dpeaa)DE-He213 Vesicular transport (dpeaa)DE-He213 Intracellular trafficking (dpeaa)DE-He213 Lysosomes (dpeaa)DE-He213 Nielsen, Simone S. E. aut Tomaka, Weronika aut Abbott, N. Joan aut Nielsen, Morten S. aut Enthalten in Cerebrospinal fluid research London : BioMed Central, 2004 16(2019), 1 vom: 30. Mai (DE-627)476171717 (DE-600)2171132-X 1743-8454 nnns volume:16 year:2019 number:1 day:30 month:05 https://dx.doi.org/10.1186/s12987-019-0134-9 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_2003 AR 16 2019 1 30 05 |
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10.1186/s12987-019-0134-9 doi (DE-627)SPR029293324 (SPR)s12987-019-0134-9-e DE-627 ger DE-627 rakwb eng Toth, Andrea E. verfasserin (orcid)0000-0001-6122-919X aut The endo-lysosomal system of bEnd.3 and hCMEC/D3 brain endothelial cells 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2019 Background Brain endothelial cell-based in vitro models are among the most versatile tools in blood–brain barrier research for testing drug penetration to the central nervous system. Transcytosis of large pharmaceuticals across the brain capillary endothelium involves the complex endo-lysosomal system. This system consists of several types of vesicle, such as early, late and recycling endosomes, retromer-positive structures, and lysosomes. Since the endo-lysosomal system in endothelial cell lines of in vitro blood–brain barrier models has not been investigated in detail, our aim was to characterize this system in different models. Methods For the investigation, we have chosen two widely-used models for in vitro drug transport studies: the bEnd.3 mouse and the hCMEC/D3 human brain endothelial cell line. We compared the structures and attributes of their endo-lysosomal system to that of primary porcine brain endothelial cells. Results We detected significant differences in the vesicular network regarding number, morphology, subcellular distribution and lysosomal activity. The retromer-positive vesicles of the primary cells were distinct in many ways from those of the cell lines. However, the cell lines showed higher lysosomal degradation activity than the primary cells. Additionally, the hCMEC/D3 possessed a strikingly unique ratio of recycling endosomes to late endosomes. Conclusions Taken together our data identify differences in the trafficking network of brain endothelial cells, essentially mapping the endo-lysosomal system of in vitro blood–brain barrier models. This knowledge is valuable for planning the optimal route across the blood–brain barrier and advancing drug delivery to the brain. bEnd.3 mouse brain endothelial cell line (dpeaa)DE-He213 hCMEC/D3 human brain endothelial cell line (dpeaa)DE-He213 Primary porcine brain endothelial cell (dpeaa)DE-He213 Blood–brain barrier (dpeaa)DE-He213 Endo-lysosomal system (dpeaa)DE-He213 Vesicular transport (dpeaa)DE-He213 Intracellular trafficking (dpeaa)DE-He213 Lysosomes (dpeaa)DE-He213 Nielsen, Simone S. E. aut Tomaka, Weronika aut Abbott, N. Joan aut Nielsen, Morten S. aut Enthalten in Cerebrospinal fluid research London : BioMed Central, 2004 16(2019), 1 vom: 30. Mai (DE-627)476171717 (DE-600)2171132-X 1743-8454 nnns volume:16 year:2019 number:1 day:30 month:05 https://dx.doi.org/10.1186/s12987-019-0134-9 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_2003 AR 16 2019 1 30 05 |
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10.1186/s12987-019-0134-9 doi (DE-627)SPR029293324 (SPR)s12987-019-0134-9-e DE-627 ger DE-627 rakwb eng Toth, Andrea E. verfasserin (orcid)0000-0001-6122-919X aut The endo-lysosomal system of bEnd.3 and hCMEC/D3 brain endothelial cells 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2019 Background Brain endothelial cell-based in vitro models are among the most versatile tools in blood–brain barrier research for testing drug penetration to the central nervous system. Transcytosis of large pharmaceuticals across the brain capillary endothelium involves the complex endo-lysosomal system. This system consists of several types of vesicle, such as early, late and recycling endosomes, retromer-positive structures, and lysosomes. Since the endo-lysosomal system in endothelial cell lines of in vitro blood–brain barrier models has not been investigated in detail, our aim was to characterize this system in different models. Methods For the investigation, we have chosen two widely-used models for in vitro drug transport studies: the bEnd.3 mouse and the hCMEC/D3 human brain endothelial cell line. We compared the structures and attributes of their endo-lysosomal system to that of primary porcine brain endothelial cells. Results We detected significant differences in the vesicular network regarding number, morphology, subcellular distribution and lysosomal activity. The retromer-positive vesicles of the primary cells were distinct in many ways from those of the cell lines. However, the cell lines showed higher lysosomal degradation activity than the primary cells. Additionally, the hCMEC/D3 possessed a strikingly unique ratio of recycling endosomes to late endosomes. Conclusions Taken together our data identify differences in the trafficking network of brain endothelial cells, essentially mapping the endo-lysosomal system of in vitro blood–brain barrier models. This knowledge is valuable for planning the optimal route across the blood–brain barrier and advancing drug delivery to the brain. bEnd.3 mouse brain endothelial cell line (dpeaa)DE-He213 hCMEC/D3 human brain endothelial cell line (dpeaa)DE-He213 Primary porcine brain endothelial cell (dpeaa)DE-He213 Blood–brain barrier (dpeaa)DE-He213 Endo-lysosomal system (dpeaa)DE-He213 Vesicular transport (dpeaa)DE-He213 Intracellular trafficking (dpeaa)DE-He213 Lysosomes (dpeaa)DE-He213 Nielsen, Simone S. E. aut Tomaka, Weronika aut Abbott, N. Joan aut Nielsen, Morten S. aut Enthalten in Cerebrospinal fluid research London : BioMed Central, 2004 16(2019), 1 vom: 30. Mai (DE-627)476171717 (DE-600)2171132-X 1743-8454 nnns volume:16 year:2019 number:1 day:30 month:05 https://dx.doi.org/10.1186/s12987-019-0134-9 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_2003 AR 16 2019 1 30 05 |
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10.1186/s12987-019-0134-9 doi (DE-627)SPR029293324 (SPR)s12987-019-0134-9-e DE-627 ger DE-627 rakwb eng Toth, Andrea E. verfasserin (orcid)0000-0001-6122-919X aut The endo-lysosomal system of bEnd.3 and hCMEC/D3 brain endothelial cells 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2019 Background Brain endothelial cell-based in vitro models are among the most versatile tools in blood–brain barrier research for testing drug penetration to the central nervous system. Transcytosis of large pharmaceuticals across the brain capillary endothelium involves the complex endo-lysosomal system. This system consists of several types of vesicle, such as early, late and recycling endosomes, retromer-positive structures, and lysosomes. Since the endo-lysosomal system in endothelial cell lines of in vitro blood–brain barrier models has not been investigated in detail, our aim was to characterize this system in different models. Methods For the investigation, we have chosen two widely-used models for in vitro drug transport studies: the bEnd.3 mouse and the hCMEC/D3 human brain endothelial cell line. We compared the structures and attributes of their endo-lysosomal system to that of primary porcine brain endothelial cells. Results We detected significant differences in the vesicular network regarding number, morphology, subcellular distribution and lysosomal activity. The retromer-positive vesicles of the primary cells were distinct in many ways from those of the cell lines. However, the cell lines showed higher lysosomal degradation activity than the primary cells. Additionally, the hCMEC/D3 possessed a strikingly unique ratio of recycling endosomes to late endosomes. Conclusions Taken together our data identify differences in the trafficking network of brain endothelial cells, essentially mapping the endo-lysosomal system of in vitro blood–brain barrier models. This knowledge is valuable for planning the optimal route across the blood–brain barrier and advancing drug delivery to the brain. bEnd.3 mouse brain endothelial cell line (dpeaa)DE-He213 hCMEC/D3 human brain endothelial cell line (dpeaa)DE-He213 Primary porcine brain endothelial cell (dpeaa)DE-He213 Blood–brain barrier (dpeaa)DE-He213 Endo-lysosomal system (dpeaa)DE-He213 Vesicular transport (dpeaa)DE-He213 Intracellular trafficking (dpeaa)DE-He213 Lysosomes (dpeaa)DE-He213 Nielsen, Simone S. E. aut Tomaka, Weronika aut Abbott, N. Joan aut Nielsen, Morten S. aut Enthalten in Cerebrospinal fluid research London : BioMed Central, 2004 16(2019), 1 vom: 30. Mai (DE-627)476171717 (DE-600)2171132-X 1743-8454 nnns volume:16 year:2019 number:1 day:30 month:05 https://dx.doi.org/10.1186/s12987-019-0134-9 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_2003 AR 16 2019 1 30 05 |
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10.1186/s12987-019-0134-9 doi (DE-627)SPR029293324 (SPR)s12987-019-0134-9-e DE-627 ger DE-627 rakwb eng Toth, Andrea E. verfasserin (orcid)0000-0001-6122-919X aut The endo-lysosomal system of bEnd.3 and hCMEC/D3 brain endothelial cells 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2019 Background Brain endothelial cell-based in vitro models are among the most versatile tools in blood–brain barrier research for testing drug penetration to the central nervous system. Transcytosis of large pharmaceuticals across the brain capillary endothelium involves the complex endo-lysosomal system. This system consists of several types of vesicle, such as early, late and recycling endosomes, retromer-positive structures, and lysosomes. Since the endo-lysosomal system in endothelial cell lines of in vitro blood–brain barrier models has not been investigated in detail, our aim was to characterize this system in different models. Methods For the investigation, we have chosen two widely-used models for in vitro drug transport studies: the bEnd.3 mouse and the hCMEC/D3 human brain endothelial cell line. We compared the structures and attributes of their endo-lysosomal system to that of primary porcine brain endothelial cells. Results We detected significant differences in the vesicular network regarding number, morphology, subcellular distribution and lysosomal activity. The retromer-positive vesicles of the primary cells were distinct in many ways from those of the cell lines. However, the cell lines showed higher lysosomal degradation activity than the primary cells. Additionally, the hCMEC/D3 possessed a strikingly unique ratio of recycling endosomes to late endosomes. Conclusions Taken together our data identify differences in the trafficking network of brain endothelial cells, essentially mapping the endo-lysosomal system of in vitro blood–brain barrier models. This knowledge is valuable for planning the optimal route across the blood–brain barrier and advancing drug delivery to the brain. bEnd.3 mouse brain endothelial cell line (dpeaa)DE-He213 hCMEC/D3 human brain endothelial cell line (dpeaa)DE-He213 Primary porcine brain endothelial cell (dpeaa)DE-He213 Blood–brain barrier (dpeaa)DE-He213 Endo-lysosomal system (dpeaa)DE-He213 Vesicular transport (dpeaa)DE-He213 Intracellular trafficking (dpeaa)DE-He213 Lysosomes (dpeaa)DE-He213 Nielsen, Simone S. E. aut Tomaka, Weronika aut Abbott, N. Joan aut Nielsen, Morten S. aut Enthalten in Cerebrospinal fluid research London : BioMed Central, 2004 16(2019), 1 vom: 30. Mai (DE-627)476171717 (DE-600)2171132-X 1743-8454 nnns volume:16 year:2019 number:1 day:30 month:05 https://dx.doi.org/10.1186/s12987-019-0134-9 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_2003 AR 16 2019 1 30 05 |
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Toth, Andrea E. misc bEnd.3 mouse brain endothelial cell line misc hCMEC/D3 human brain endothelial cell line misc Primary porcine brain endothelial cell misc Blood–brain barrier misc Endo-lysosomal system misc Vesicular transport misc Intracellular trafficking misc Lysosomes The endo-lysosomal system of bEnd.3 and hCMEC/D3 brain endothelial cells |
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The endo-lysosomal system of bEnd.3 and hCMEC/D3 brain endothelial cells bEnd.3 mouse brain endothelial cell line (dpeaa)DE-He213 hCMEC/D3 human brain endothelial cell line (dpeaa)DE-He213 Primary porcine brain endothelial cell (dpeaa)DE-He213 Blood–brain barrier (dpeaa)DE-He213 Endo-lysosomal system (dpeaa)DE-He213 Vesicular transport (dpeaa)DE-He213 Intracellular trafficking (dpeaa)DE-He213 Lysosomes (dpeaa)DE-He213 |
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Toth, Andrea E. Nielsen, Simone S. E. Tomaka, Weronika Abbott, N. Joan Nielsen, Morten S. |
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endo-lysosomal system of bend.3 and hcmec/d3 brain endothelial cells |
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The endo-lysosomal system of bEnd.3 and hCMEC/D3 brain endothelial cells |
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Background Brain endothelial cell-based in vitro models are among the most versatile tools in blood–brain barrier research for testing drug penetration to the central nervous system. Transcytosis of large pharmaceuticals across the brain capillary endothelium involves the complex endo-lysosomal system. This system consists of several types of vesicle, such as early, late and recycling endosomes, retromer-positive structures, and lysosomes. Since the endo-lysosomal system in endothelial cell lines of in vitro blood–brain barrier models has not been investigated in detail, our aim was to characterize this system in different models. Methods For the investigation, we have chosen two widely-used models for in vitro drug transport studies: the bEnd.3 mouse and the hCMEC/D3 human brain endothelial cell line. We compared the structures and attributes of their endo-lysosomal system to that of primary porcine brain endothelial cells. Results We detected significant differences in the vesicular network regarding number, morphology, subcellular distribution and lysosomal activity. The retromer-positive vesicles of the primary cells were distinct in many ways from those of the cell lines. However, the cell lines showed higher lysosomal degradation activity than the primary cells. Additionally, the hCMEC/D3 possessed a strikingly unique ratio of recycling endosomes to late endosomes. Conclusions Taken together our data identify differences in the trafficking network of brain endothelial cells, essentially mapping the endo-lysosomal system of in vitro blood–brain barrier models. This knowledge is valuable for planning the optimal route across the blood–brain barrier and advancing drug delivery to the brain. © The Author(s) 2019 |
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Background Brain endothelial cell-based in vitro models are among the most versatile tools in blood–brain barrier research for testing drug penetration to the central nervous system. Transcytosis of large pharmaceuticals across the brain capillary endothelium involves the complex endo-lysosomal system. This system consists of several types of vesicle, such as early, late and recycling endosomes, retromer-positive structures, and lysosomes. Since the endo-lysosomal system in endothelial cell lines of in vitro blood–brain barrier models has not been investigated in detail, our aim was to characterize this system in different models. Methods For the investigation, we have chosen two widely-used models for in vitro drug transport studies: the bEnd.3 mouse and the hCMEC/D3 human brain endothelial cell line. We compared the structures and attributes of their endo-lysosomal system to that of primary porcine brain endothelial cells. Results We detected significant differences in the vesicular network regarding number, morphology, subcellular distribution and lysosomal activity. The retromer-positive vesicles of the primary cells were distinct in many ways from those of the cell lines. However, the cell lines showed higher lysosomal degradation activity than the primary cells. Additionally, the hCMEC/D3 possessed a strikingly unique ratio of recycling endosomes to late endosomes. Conclusions Taken together our data identify differences in the trafficking network of brain endothelial cells, essentially mapping the endo-lysosomal system of in vitro blood–brain barrier models. This knowledge is valuable for planning the optimal route across the blood–brain barrier and advancing drug delivery to the brain. © The Author(s) 2019 |
| abstract_unstemmed |
Background Brain endothelial cell-based in vitro models are among the most versatile tools in blood–brain barrier research for testing drug penetration to the central nervous system. Transcytosis of large pharmaceuticals across the brain capillary endothelium involves the complex endo-lysosomal system. This system consists of several types of vesicle, such as early, late and recycling endosomes, retromer-positive structures, and lysosomes. Since the endo-lysosomal system in endothelial cell lines of in vitro blood–brain barrier models has not been investigated in detail, our aim was to characterize this system in different models. Methods For the investigation, we have chosen two widely-used models for in vitro drug transport studies: the bEnd.3 mouse and the hCMEC/D3 human brain endothelial cell line. We compared the structures and attributes of their endo-lysosomal system to that of primary porcine brain endothelial cells. Results We detected significant differences in the vesicular network regarding number, morphology, subcellular distribution and lysosomal activity. The retromer-positive vesicles of the primary cells were distinct in many ways from those of the cell lines. However, the cell lines showed higher lysosomal degradation activity than the primary cells. Additionally, the hCMEC/D3 possessed a strikingly unique ratio of recycling endosomes to late endosomes. Conclusions Taken together our data identify differences in the trafficking network of brain endothelial cells, essentially mapping the endo-lysosomal system of in vitro blood–brain barrier models. This knowledge is valuable for planning the optimal route across the blood–brain barrier and advancing drug delivery to the brain. © The Author(s) 2019 |
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