Analysis of epithelial-mesenchymal transition markers in the histogenesis of hepatic progenitor cell in HBV-related liver diseases

Background The origin and heterogeneity of hepatic progenitor cells (HPCs) remain unclear. This study aimed to investigate the involvement of epithelial-mesenchymal transition (EMT) in the histogenesis of HPCs. Methods Surgical liver specimens from patients with HBV-related hepatitis and cirrhosis w...
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Autor*in:	Xu, Wei [verfasserIn] Wang, Nong-Rong Wang, Hua-Feng Feng, Qiong Deng, Jun Gong, Zhi-Qiang Sun, Jian Lou, Xiao-Liang Yu, Xue-Feng Zhou, Lv Hu, Jin-Ping Huang, Xiao-Feng Qi, Xiao-Qing Deng, Yan-Juan Gong, Rui Guo, Yan Wang, Meng-Meng Xiao, Jia-Cheng Deng, Huan

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2016

Schlagwörter:	Hepatic progenitor cell Epithelial-mesenchymal transition HBV Cholangiocyte Histogenesis

Anmerkung:	© The Author(s). 2016

Übergeordnetes Werk:	Enthalten in: Diagnostic pathology - [S.l.] : BioMed Central, 2006, 11(2016), 1 vom: 24. Nov.
Übergeordnetes Werk:	volume:11 ; year:2016 ; number:1 ; day:24 ; month:11

Links:	Volltext

DOI / URN:	10.1186/s13000-016-0587-y

Katalog-ID:	SPR029379342

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520			\|a Background The origin and heterogeneity of hepatic progenitor cells (HPCs) remain unclear. This study aimed to investigate the involvement of epithelial-mesenchymal transition (EMT) in the histogenesis of HPCs. Methods Surgical liver specimens from patients with HBV-related hepatitis and cirrhosis were investigated with double immunofluorescence labeling to detect antigens associated with HPCs and EMT. Ductular reactions were subjected to quantitative reverse transcription PCR following isolation by laser capture microdissection. Electron microscopic examination was performed to find an ultrastructural evidence of EMT. Results The number of EpCAM-positive HPCs was proportional to the disease severity. The S100A4 expression of HPCs was firstly observed in mild hepatitis and increased significantly in moderate hepatitis, but decreased in severe hepatitis and cirrhosis. The levels of MMP-2, Twist, and Snail increased in direct proportion to the number of HPCs. Some hepatocytes adjacent to portal tracts in cirrhosis showed positivity for MMP-2. Although CK7 and E-cadherin levels decreased in mild and moderate hepatitis, HPCs re-expressed both of them in severe hepatitis and cirrhosis. However, HPCs expressed neither vimentin nor αSMA. The relative mRNA expression levels of EpCAM and EMT-associated markers supported immunohistochemical results. Electron microscopic examination demonstrated the existence of intercellular junctions among HPCs, cholangiocytes, and intermediate hepatocyte-like cells. Conclusion We provided preliminary evidence for the involvement of EMT in the histogenesis of HPCs from cholangiocytes in HBV-related liver diseases. HPCs may re-transdifferentiate into hepatocytes, and the differentiation direction depends, at least in part, on interactions between HPCs and the surrounding microenvironment, especially the non-resolving inflammation caused by HBV infection.
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allfields	10.1186/s13000-016-0587-y doi (DE-627)SPR029379342 (SPR)s13000-016-0587-y-e DE-627 ger DE-627 rakwb eng Xu, Wei verfasserin aut Analysis of epithelial-mesenchymal transition markers in the histogenesis of hepatic progenitor cell in HBV-related liver diseases 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2016 Background The origin and heterogeneity of hepatic progenitor cells (HPCs) remain unclear. This study aimed to investigate the involvement of epithelial-mesenchymal transition (EMT) in the histogenesis of HPCs. Methods Surgical liver specimens from patients with HBV-related hepatitis and cirrhosis were investigated with double immunofluorescence labeling to detect antigens associated with HPCs and EMT. Ductular reactions were subjected to quantitative reverse transcription PCR following isolation by laser capture microdissection. Electron microscopic examination was performed to find an ultrastructural evidence of EMT. Results The number of EpCAM-positive HPCs was proportional to the disease severity. The S100A4 expression of HPCs was firstly observed in mild hepatitis and increased significantly in moderate hepatitis, but decreased in severe hepatitis and cirrhosis. The levels of MMP-2, Twist, and Snail increased in direct proportion to the number of HPCs. Some hepatocytes adjacent to portal tracts in cirrhosis showed positivity for MMP-2. Although CK7 and E-cadherin levels decreased in mild and moderate hepatitis, HPCs re-expressed both of them in severe hepatitis and cirrhosis. However, HPCs expressed neither vimentin nor αSMA. The relative mRNA expression levels of EpCAM and EMT-associated markers supported immunohistochemical results. Electron microscopic examination demonstrated the existence of intercellular junctions among HPCs, cholangiocytes, and intermediate hepatocyte-like cells. Conclusion We provided preliminary evidence for the involvement of EMT in the histogenesis of HPCs from cholangiocytes in HBV-related liver diseases. HPCs may re-transdifferentiate into hepatocytes, and the differentiation direction depends, at least in part, on interactions between HPCs and the surrounding microenvironment, especially the non-resolving inflammation caused by HBV infection. Hepatic progenitor cell (dpeaa)DE-He213 Epithelial-mesenchymal transition (dpeaa)DE-He213 HBV (dpeaa)DE-He213 Cholangiocyte (dpeaa)DE-He213 Histogenesis (dpeaa)DE-He213 Wang, Nong-Rong aut Wang, Hua-Feng aut Feng, Qiong aut Deng, Jun aut Gong, Zhi-Qiang aut Sun, Jian aut Lou, Xiao-Liang aut Yu, Xue-Feng aut Zhou, Lv aut Hu, Jin-Ping aut Huang, Xiao-Feng aut Qi, Xiao-Qing aut Deng, Yan-Juan aut Gong, Rui aut Guo, Yan aut Wang, Meng-Meng aut Xiao, Jia-Cheng aut Deng, Huan (orcid)0000-0002-1596-2013 aut Enthalten in Diagnostic pathology [S.l.] : BioMed Central, 2006 11(2016), 1 vom: 24. Nov. (DE-627)503328960 (DE-600)2210518-9 1746-1596 nnns volume:11 year:2016 number:1 day:24 month:11 https://dx.doi.org/10.1186/s13000-016-0587-y kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_168 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2016 1 24 11
spelling	10.1186/s13000-016-0587-y doi (DE-627)SPR029379342 (SPR)s13000-016-0587-y-e DE-627 ger DE-627 rakwb eng Xu, Wei verfasserin aut Analysis of epithelial-mesenchymal transition markers in the histogenesis of hepatic progenitor cell in HBV-related liver diseases 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2016 Background The origin and heterogeneity of hepatic progenitor cells (HPCs) remain unclear. This study aimed to investigate the involvement of epithelial-mesenchymal transition (EMT) in the histogenesis of HPCs. Methods Surgical liver specimens from patients with HBV-related hepatitis and cirrhosis were investigated with double immunofluorescence labeling to detect antigens associated with HPCs and EMT. Ductular reactions were subjected to quantitative reverse transcription PCR following isolation by laser capture microdissection. Electron microscopic examination was performed to find an ultrastructural evidence of EMT. Results The number of EpCAM-positive HPCs was proportional to the disease severity. The S100A4 expression of HPCs was firstly observed in mild hepatitis and increased significantly in moderate hepatitis, but decreased in severe hepatitis and cirrhosis. The levels of MMP-2, Twist, and Snail increased in direct proportion to the number of HPCs. Some hepatocytes adjacent to portal tracts in cirrhosis showed positivity for MMP-2. Although CK7 and E-cadherin levels decreased in mild and moderate hepatitis, HPCs re-expressed both of them in severe hepatitis and cirrhosis. However, HPCs expressed neither vimentin nor αSMA. The relative mRNA expression levels of EpCAM and EMT-associated markers supported immunohistochemical results. Electron microscopic examination demonstrated the existence of intercellular junctions among HPCs, cholangiocytes, and intermediate hepatocyte-like cells. Conclusion We provided preliminary evidence for the involvement of EMT in the histogenesis of HPCs from cholangiocytes in HBV-related liver diseases. HPCs may re-transdifferentiate into hepatocytes, and the differentiation direction depends, at least in part, on interactions between HPCs and the surrounding microenvironment, especially the non-resolving inflammation caused by HBV infection. Hepatic progenitor cell (dpeaa)DE-He213 Epithelial-mesenchymal transition (dpeaa)DE-He213 HBV (dpeaa)DE-He213 Cholangiocyte (dpeaa)DE-He213 Histogenesis (dpeaa)DE-He213 Wang, Nong-Rong aut Wang, Hua-Feng aut Feng, Qiong aut Deng, Jun aut Gong, Zhi-Qiang aut Sun, Jian aut Lou, Xiao-Liang aut Yu, Xue-Feng aut Zhou, Lv aut Hu, Jin-Ping aut Huang, Xiao-Feng aut Qi, Xiao-Qing aut Deng, Yan-Juan aut Gong, Rui aut Guo, Yan aut Wang, Meng-Meng aut Xiao, Jia-Cheng aut Deng, Huan (orcid)0000-0002-1596-2013 aut Enthalten in Diagnostic pathology [S.l.] : BioMed Central, 2006 11(2016), 1 vom: 24. Nov. (DE-627)503328960 (DE-600)2210518-9 1746-1596 nnns volume:11 year:2016 number:1 day:24 month:11 https://dx.doi.org/10.1186/s13000-016-0587-y kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_168 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2016 1 24 11
allfields_unstemmed	10.1186/s13000-016-0587-y doi (DE-627)SPR029379342 (SPR)s13000-016-0587-y-e DE-627 ger DE-627 rakwb eng Xu, Wei verfasserin aut Analysis of epithelial-mesenchymal transition markers in the histogenesis of hepatic progenitor cell in HBV-related liver diseases 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2016 Background The origin and heterogeneity of hepatic progenitor cells (HPCs) remain unclear. This study aimed to investigate the involvement of epithelial-mesenchymal transition (EMT) in the histogenesis of HPCs. Methods Surgical liver specimens from patients with HBV-related hepatitis and cirrhosis were investigated with double immunofluorescence labeling to detect antigens associated with HPCs and EMT. Ductular reactions were subjected to quantitative reverse transcription PCR following isolation by laser capture microdissection. Electron microscopic examination was performed to find an ultrastructural evidence of EMT. Results The number of EpCAM-positive HPCs was proportional to the disease severity. The S100A4 expression of HPCs was firstly observed in mild hepatitis and increased significantly in moderate hepatitis, but decreased in severe hepatitis and cirrhosis. The levels of MMP-2, Twist, and Snail increased in direct proportion to the number of HPCs. Some hepatocytes adjacent to portal tracts in cirrhosis showed positivity for MMP-2. Although CK7 and E-cadherin levels decreased in mild and moderate hepatitis, HPCs re-expressed both of them in severe hepatitis and cirrhosis. However, HPCs expressed neither vimentin nor αSMA. The relative mRNA expression levels of EpCAM and EMT-associated markers supported immunohistochemical results. Electron microscopic examination demonstrated the existence of intercellular junctions among HPCs, cholangiocytes, and intermediate hepatocyte-like cells. Conclusion We provided preliminary evidence for the involvement of EMT in the histogenesis of HPCs from cholangiocytes in HBV-related liver diseases. HPCs may re-transdifferentiate into hepatocytes, and the differentiation direction depends, at least in part, on interactions between HPCs and the surrounding microenvironment, especially the non-resolving inflammation caused by HBV infection. Hepatic progenitor cell (dpeaa)DE-He213 Epithelial-mesenchymal transition (dpeaa)DE-He213 HBV (dpeaa)DE-He213 Cholangiocyte (dpeaa)DE-He213 Histogenesis (dpeaa)DE-He213 Wang, Nong-Rong aut Wang, Hua-Feng aut Feng, Qiong aut Deng, Jun aut Gong, Zhi-Qiang aut Sun, Jian aut Lou, Xiao-Liang aut Yu, Xue-Feng aut Zhou, Lv aut Hu, Jin-Ping aut Huang, Xiao-Feng aut Qi, Xiao-Qing aut Deng, Yan-Juan aut Gong, Rui aut Guo, Yan aut Wang, Meng-Meng aut Xiao, Jia-Cheng aut Deng, Huan (orcid)0000-0002-1596-2013 aut Enthalten in Diagnostic pathology [S.l.] : BioMed Central, 2006 11(2016), 1 vom: 24. Nov. (DE-627)503328960 (DE-600)2210518-9 1746-1596 nnns volume:11 year:2016 number:1 day:24 month:11 https://dx.doi.org/10.1186/s13000-016-0587-y kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_168 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2016 1 24 11
allfieldsGer	10.1186/s13000-016-0587-y doi (DE-627)SPR029379342 (SPR)s13000-016-0587-y-e DE-627 ger DE-627 rakwb eng Xu, Wei verfasserin aut Analysis of epithelial-mesenchymal transition markers in the histogenesis of hepatic progenitor cell in HBV-related liver diseases 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2016 Background The origin and heterogeneity of hepatic progenitor cells (HPCs) remain unclear. This study aimed to investigate the involvement of epithelial-mesenchymal transition (EMT) in the histogenesis of HPCs. Methods Surgical liver specimens from patients with HBV-related hepatitis and cirrhosis were investigated with double immunofluorescence labeling to detect antigens associated with HPCs and EMT. Ductular reactions were subjected to quantitative reverse transcription PCR following isolation by laser capture microdissection. Electron microscopic examination was performed to find an ultrastructural evidence of EMT. Results The number of EpCAM-positive HPCs was proportional to the disease severity. The S100A4 expression of HPCs was firstly observed in mild hepatitis and increased significantly in moderate hepatitis, but decreased in severe hepatitis and cirrhosis. The levels of MMP-2, Twist, and Snail increased in direct proportion to the number of HPCs. Some hepatocytes adjacent to portal tracts in cirrhosis showed positivity for MMP-2. Although CK7 and E-cadherin levels decreased in mild and moderate hepatitis, HPCs re-expressed both of them in severe hepatitis and cirrhosis. However, HPCs expressed neither vimentin nor αSMA. The relative mRNA expression levels of EpCAM and EMT-associated markers supported immunohistochemical results. Electron microscopic examination demonstrated the existence of intercellular junctions among HPCs, cholangiocytes, and intermediate hepatocyte-like cells. Conclusion We provided preliminary evidence for the involvement of EMT in the histogenesis of HPCs from cholangiocytes in HBV-related liver diseases. HPCs may re-transdifferentiate into hepatocytes, and the differentiation direction depends, at least in part, on interactions between HPCs and the surrounding microenvironment, especially the non-resolving inflammation caused by HBV infection. Hepatic progenitor cell (dpeaa)DE-He213 Epithelial-mesenchymal transition (dpeaa)DE-He213 HBV (dpeaa)DE-He213 Cholangiocyte (dpeaa)DE-He213 Histogenesis (dpeaa)DE-He213 Wang, Nong-Rong aut Wang, Hua-Feng aut Feng, Qiong aut Deng, Jun aut Gong, Zhi-Qiang aut Sun, Jian aut Lou, Xiao-Liang aut Yu, Xue-Feng aut Zhou, Lv aut Hu, Jin-Ping aut Huang, Xiao-Feng aut Qi, Xiao-Qing aut Deng, Yan-Juan aut Gong, Rui aut Guo, Yan aut Wang, Meng-Meng aut Xiao, Jia-Cheng aut Deng, Huan (orcid)0000-0002-1596-2013 aut Enthalten in Diagnostic pathology [S.l.] : BioMed Central, 2006 11(2016), 1 vom: 24. Nov. (DE-627)503328960 (DE-600)2210518-9 1746-1596 nnns volume:11 year:2016 number:1 day:24 month:11 https://dx.doi.org/10.1186/s13000-016-0587-y kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_168 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2016 1 24 11
allfieldsSound	10.1186/s13000-016-0587-y doi (DE-627)SPR029379342 (SPR)s13000-016-0587-y-e DE-627 ger DE-627 rakwb eng Xu, Wei verfasserin aut Analysis of epithelial-mesenchymal transition markers in the histogenesis of hepatic progenitor cell in HBV-related liver diseases 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2016 Background The origin and heterogeneity of hepatic progenitor cells (HPCs) remain unclear. This study aimed to investigate the involvement of epithelial-mesenchymal transition (EMT) in the histogenesis of HPCs. Methods Surgical liver specimens from patients with HBV-related hepatitis and cirrhosis were investigated with double immunofluorescence labeling to detect antigens associated with HPCs and EMT. Ductular reactions were subjected to quantitative reverse transcription PCR following isolation by laser capture microdissection. Electron microscopic examination was performed to find an ultrastructural evidence of EMT. Results The number of EpCAM-positive HPCs was proportional to the disease severity. The S100A4 expression of HPCs was firstly observed in mild hepatitis and increased significantly in moderate hepatitis, but decreased in severe hepatitis and cirrhosis. The levels of MMP-2, Twist, and Snail increased in direct proportion to the number of HPCs. Some hepatocytes adjacent to portal tracts in cirrhosis showed positivity for MMP-2. Although CK7 and E-cadherin levels decreased in mild and moderate hepatitis, HPCs re-expressed both of them in severe hepatitis and cirrhosis. However, HPCs expressed neither vimentin nor αSMA. The relative mRNA expression levels of EpCAM and EMT-associated markers supported immunohistochemical results. Electron microscopic examination demonstrated the existence of intercellular junctions among HPCs, cholangiocytes, and intermediate hepatocyte-like cells. Conclusion We provided preliminary evidence for the involvement of EMT in the histogenesis of HPCs from cholangiocytes in HBV-related liver diseases. HPCs may re-transdifferentiate into hepatocytes, and the differentiation direction depends, at least in part, on interactions between HPCs and the surrounding microenvironment, especially the non-resolving inflammation caused by HBV infection. Hepatic progenitor cell (dpeaa)DE-He213 Epithelial-mesenchymal transition (dpeaa)DE-He213 HBV (dpeaa)DE-He213 Cholangiocyte (dpeaa)DE-He213 Histogenesis (dpeaa)DE-He213 Wang, Nong-Rong aut Wang, Hua-Feng aut Feng, Qiong aut Deng, Jun aut Gong, Zhi-Qiang aut Sun, Jian aut Lou, Xiao-Liang aut Yu, Xue-Feng aut Zhou, Lv aut Hu, Jin-Ping aut Huang, Xiao-Feng aut Qi, Xiao-Qing aut Deng, Yan-Juan aut Gong, Rui aut Guo, Yan aut Wang, Meng-Meng aut Xiao, Jia-Cheng aut Deng, Huan (orcid)0000-0002-1596-2013 aut Enthalten in Diagnostic pathology [S.l.] : BioMed Central, 2006 11(2016), 1 vom: 24. Nov. (DE-627)503328960 (DE-600)2210518-9 1746-1596 nnns volume:11 year:2016 number:1 day:24 month:11 https://dx.doi.org/10.1186/s13000-016-0587-y kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_168 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2016 1 24 11
language	English
source	Enthalten in Diagnostic pathology 11(2016), 1 vom: 24. Nov. volume:11 year:2016 number:1 day:24 month:11
sourceStr	Enthalten in Diagnostic pathology 11(2016), 1 vom: 24. Nov. volume:11 year:2016 number:1 day:24 month:11
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Hepatic progenitor cell Epithelial-mesenchymal transition HBV Cholangiocyte Histogenesis
isfreeaccess_bool	true
container_title	Diagnostic pathology
authorswithroles_txt_mv	Xu, Wei @@aut@@ Wang, Nong-Rong @@aut@@ Wang, Hua-Feng @@aut@@ Feng, Qiong @@aut@@ Deng, Jun @@aut@@ Gong, Zhi-Qiang @@aut@@ Sun, Jian @@aut@@ Lou, Xiao-Liang @@aut@@ Yu, Xue-Feng @@aut@@ Zhou, Lv @@aut@@ Hu, Jin-Ping @@aut@@ Huang, Xiao-Feng @@aut@@ Qi, Xiao-Qing @@aut@@ Deng, Yan-Juan @@aut@@ Gong, Rui @@aut@@ Guo, Yan @@aut@@ Wang, Meng-Meng @@aut@@ Xiao, Jia-Cheng @@aut@@ Deng, Huan @@aut@@
publishDateDaySort_date	2016-11-24T00:00:00Z
hierarchy_top_id	503328960
id	SPR029379342
language_de	englisch
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author	Xu, Wei
spellingShingle	Xu, Wei misc Hepatic progenitor cell misc Epithelial-mesenchymal transition misc HBV misc Cholangiocyte misc Histogenesis Analysis of epithelial-mesenchymal transition markers in the histogenesis of hepatic progenitor cell in HBV-related liver diseases
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topic_title	Analysis of epithelial-mesenchymal transition markers in the histogenesis of hepatic progenitor cell in HBV-related liver diseases Hepatic progenitor cell (dpeaa)DE-He213 Epithelial-mesenchymal transition (dpeaa)DE-He213 HBV (dpeaa)DE-He213 Cholangiocyte (dpeaa)DE-He213 Histogenesis (dpeaa)DE-He213
topic	misc Hepatic progenitor cell misc Epithelial-mesenchymal transition misc HBV misc Cholangiocyte misc Histogenesis
topic_unstemmed	misc Hepatic progenitor cell misc Epithelial-mesenchymal transition misc HBV misc Cholangiocyte misc Histogenesis
topic_browse	misc Hepatic progenitor cell misc Epithelial-mesenchymal transition misc HBV misc Cholangiocyte misc Histogenesis
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title	Analysis of epithelial-mesenchymal transition markers in the histogenesis of hepatic progenitor cell in HBV-related liver diseases
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title_full	Analysis of epithelial-mesenchymal transition markers in the histogenesis of hepatic progenitor cell in HBV-related liver diseases
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journal	Diagnostic pathology
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author_browse	Xu, Wei Wang, Nong-Rong Wang, Hua-Feng Feng, Qiong Deng, Jun Gong, Zhi-Qiang Sun, Jian Lou, Xiao-Liang Yu, Xue-Feng Zhou, Lv Hu, Jin-Ping Huang, Xiao-Feng Qi, Xiao-Qing Deng, Yan-Juan Gong, Rui Guo, Yan Wang, Meng-Meng Xiao, Jia-Cheng Deng, Huan
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title_sort	analysis of epithelial-mesenchymal transition markers in the histogenesis of hepatic progenitor cell in hbv-related liver diseases
title_auth	Analysis of epithelial-mesenchymal transition markers in the histogenesis of hepatic progenitor cell in HBV-related liver diseases
abstract	Background The origin and heterogeneity of hepatic progenitor cells (HPCs) remain unclear. This study aimed to investigate the involvement of epithelial-mesenchymal transition (EMT) in the histogenesis of HPCs. Methods Surgical liver specimens from patients with HBV-related hepatitis and cirrhosis were investigated with double immunofluorescence labeling to detect antigens associated with HPCs and EMT. Ductular reactions were subjected to quantitative reverse transcription PCR following isolation by laser capture microdissection. Electron microscopic examination was performed to find an ultrastructural evidence of EMT. Results The number of EpCAM-positive HPCs was proportional to the disease severity. The S100A4 expression of HPCs was firstly observed in mild hepatitis and increased significantly in moderate hepatitis, but decreased in severe hepatitis and cirrhosis. The levels of MMP-2, Twist, and Snail increased in direct proportion to the number of HPCs. Some hepatocytes adjacent to portal tracts in cirrhosis showed positivity for MMP-2. Although CK7 and E-cadherin levels decreased in mild and moderate hepatitis, HPCs re-expressed both of them in severe hepatitis and cirrhosis. However, HPCs expressed neither vimentin nor αSMA. The relative mRNA expression levels of EpCAM and EMT-associated markers supported immunohistochemical results. Electron microscopic examination demonstrated the existence of intercellular junctions among HPCs, cholangiocytes, and intermediate hepatocyte-like cells. Conclusion We provided preliminary evidence for the involvement of EMT in the histogenesis of HPCs from cholangiocytes in HBV-related liver diseases. HPCs may re-transdifferentiate into hepatocytes, and the differentiation direction depends, at least in part, on interactions between HPCs and the surrounding microenvironment, especially the non-resolving inflammation caused by HBV infection. © The Author(s). 2016
abstractGer	Background The origin and heterogeneity of hepatic progenitor cells (HPCs) remain unclear. This study aimed to investigate the involvement of epithelial-mesenchymal transition (EMT) in the histogenesis of HPCs. Methods Surgical liver specimens from patients with HBV-related hepatitis and cirrhosis were investigated with double immunofluorescence labeling to detect antigens associated with HPCs and EMT. Ductular reactions were subjected to quantitative reverse transcription PCR following isolation by laser capture microdissection. Electron microscopic examination was performed to find an ultrastructural evidence of EMT. Results The number of EpCAM-positive HPCs was proportional to the disease severity. The S100A4 expression of HPCs was firstly observed in mild hepatitis and increased significantly in moderate hepatitis, but decreased in severe hepatitis and cirrhosis. The levels of MMP-2, Twist, and Snail increased in direct proportion to the number of HPCs. Some hepatocytes adjacent to portal tracts in cirrhosis showed positivity for MMP-2. Although CK7 and E-cadherin levels decreased in mild and moderate hepatitis, HPCs re-expressed both of them in severe hepatitis and cirrhosis. However, HPCs expressed neither vimentin nor αSMA. The relative mRNA expression levels of EpCAM and EMT-associated markers supported immunohistochemical results. Electron microscopic examination demonstrated the existence of intercellular junctions among HPCs, cholangiocytes, and intermediate hepatocyte-like cells. Conclusion We provided preliminary evidence for the involvement of EMT in the histogenesis of HPCs from cholangiocytes in HBV-related liver diseases. HPCs may re-transdifferentiate into hepatocytes, and the differentiation direction depends, at least in part, on interactions between HPCs and the surrounding microenvironment, especially the non-resolving inflammation caused by HBV infection. © The Author(s). 2016
abstract_unstemmed	Background The origin and heterogeneity of hepatic progenitor cells (HPCs) remain unclear. This study aimed to investigate the involvement of epithelial-mesenchymal transition (EMT) in the histogenesis of HPCs. Methods Surgical liver specimens from patients with HBV-related hepatitis and cirrhosis were investigated with double immunofluorescence labeling to detect antigens associated with HPCs and EMT. Ductular reactions were subjected to quantitative reverse transcription PCR following isolation by laser capture microdissection. Electron microscopic examination was performed to find an ultrastructural evidence of EMT. Results The number of EpCAM-positive HPCs was proportional to the disease severity. The S100A4 expression of HPCs was firstly observed in mild hepatitis and increased significantly in moderate hepatitis, but decreased in severe hepatitis and cirrhosis. The levels of MMP-2, Twist, and Snail increased in direct proportion to the number of HPCs. Some hepatocytes adjacent to portal tracts in cirrhosis showed positivity for MMP-2. Although CK7 and E-cadherin levels decreased in mild and moderate hepatitis, HPCs re-expressed both of them in severe hepatitis and cirrhosis. However, HPCs expressed neither vimentin nor αSMA. The relative mRNA expression levels of EpCAM and EMT-associated markers supported immunohistochemical results. Electron microscopic examination demonstrated the existence of intercellular junctions among HPCs, cholangiocytes, and intermediate hepatocyte-like cells. Conclusion We provided preliminary evidence for the involvement of EMT in the histogenesis of HPCs from cholangiocytes in HBV-related liver diseases. HPCs may re-transdifferentiate into hepatocytes, and the differentiation direction depends, at least in part, on interactions between HPCs and the surrounding microenvironment, especially the non-resolving inflammation caused by HBV infection. © The Author(s). 2016
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title_short	Analysis of epithelial-mesenchymal transition markers in the histogenesis of hepatic progenitor cell in HBV-related liver diseases
url	https://dx.doi.org/10.1186/s13000-016-0587-y
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author2	Wang, Nong-Rong Wang, Hua-Feng Feng, Qiong Deng, Jun Gong, Zhi-Qiang Sun, Jian Lou, Xiao-Liang Yu, Xue-Feng Zhou, Lv Hu, Jin-Ping Huang, Xiao-Feng Qi, Xiao-Qing Deng, Yan-Juan Gong, Rui Guo, Yan Wang, Meng-Meng Xiao, Jia-Cheng Deng, Huan
author2Str	Wang, Nong-Rong Wang, Hua-Feng Feng, Qiong Deng, Jun Gong, Zhi-Qiang Sun, Jian Lou, Xiao-Liang Yu, Xue-Feng Zhou, Lv Hu, Jin-Ping Huang, Xiao-Feng Qi, Xiao-Qing Deng, Yan-Juan Gong, Rui Guo, Yan Wang, Meng-Meng Xiao, Jia-Cheng Deng, Huan
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Analysis of epithelial-mesenchymal transition markers in the histogenesis of hepatic progenitor cell in HBV-related liver diseases

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