In vitro evaluation of various bioabsorbable and nonresorbable barrier membranes for guided tissue regeneration
Background Different types of bioabsorbable and nonresorbable membranes have been widely used for guided tissue regeneration (GTR) with its ultimate goal of regenerating lost periodontal structures. The purpose of the present study was to evaluate the biological effects of various bioabsorbable and...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Kasaj, Adrian [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2008 |
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| Anmerkung: |
© Kasaj et al; licensee BioMed Central Ltd. 2008 |
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| Übergeordnetes Werk: |
Enthalten in: Head & face medicine - London : BioMed Central, 2005, 4(2008), 1 vom: 14. Okt. |
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| Übergeordnetes Werk: |
volume:4 ; year:2008 ; number:1 ; day:14 ; month:10 |
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| DOI / URN: |
10.1186/1746-160X-4-22 |
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| Katalog-ID: |
SPR029393949 |
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| 520 | |a Background Different types of bioabsorbable and nonresorbable membranes have been widely used for guided tissue regeneration (GTR) with its ultimate goal of regenerating lost periodontal structures. The purpose of the present study was to evaluate the biological effects of various bioabsorbable and nonresorbable membranes in cultures of primary human gingival fibroblasts (HGF), periodontal ligament fibroblasts (PDLF) and human osteoblast-like (HOB) cells in vitro. Methods Three commercially available collagen membranes [$ TutoDent^{®} $ (TD), $ Resodont^{®} $ (RD) and $ BioGide^{®} $ (BG)] as well as three nonresorbable polytetrafluoroethylene (PTFE) membranes [ACE (AC), $ Cytoplast^{®} $ (CT) and TefGen-$ FD^{®} $ (TG)] were tested. Cells plated on culture dishes (CD) served as positive controls. The effect of the barrier membranes on HGF, PDLF as well as HOB cells was assessed by the Alamar Blue fluorometric proliferation assay after 1, 2.5, 4, 24 and 48 h time periods. The structural and morphological properties of the membranes were evaluated by scanning electron microscopy (SEM). Results The results showed that of the six barriers tested, TD and RD demonstrated the highest rate of HGF proliferation at both earlier (1 h) and later (48 h) time periods (P < 0.001) compared to all other tested barriers and CD. Similarly, TD, RD and BG had significantly higher numbers of cells at all time periods when compared with the positive control in PDLF culture (P ≤ 0.001). In HOB cell culture, the highest rate of cell proliferation was also calculated for TD at all time periods (P < 0.001). SEM observations demonstrated a microporous structure of all collagen membranes, with a compact top surface and a porous bottom surface, whereas the nonresorbable PTFE membranes demonstrated a homogenous structure with a symmetric dense skin layer. Conclusion Results from the present study suggested that GTR membrane materials, per se, may influence cell proliferation in the process of periodontal tissue/bone regeneration. Among the six membranes examined, the bioabsorbable membranes demonstrated to be more suitable to stimulate cellular proliferation compared to nonresorbable PTFE membranes. | ||
| 650 | 4 | |a Collagen Membrane |7 (dpeaa)DE-He213 | |
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| 650 | 4 | |a Guide Tissue Regeneration |7 (dpeaa)DE-He213 | |
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| 700 | 1 | |a Reichert, Christoph |4 aut | |
| 700 | 1 | |a Götz, Hermann |4 aut | |
| 700 | 1 | |a Röhrig, Bernd |4 aut | |
| 700 | 1 | |a Smeets, Ralf |4 aut | |
| 700 | 1 | |a Willershausen, Brita |4 aut | |
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2008 |
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10.1186/1746-160X-4-22 doi (DE-627)SPR029393949 (SPR)1746-160X-4-22-e DE-627 ger DE-627 rakwb eng Kasaj, Adrian verfasserin aut In vitro evaluation of various bioabsorbable and nonresorbable barrier membranes for guided tissue regeneration 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Kasaj et al; licensee BioMed Central Ltd. 2008 Background Different types of bioabsorbable and nonresorbable membranes have been widely used for guided tissue regeneration (GTR) with its ultimate goal of regenerating lost periodontal structures. The purpose of the present study was to evaluate the biological effects of various bioabsorbable and nonresorbable membranes in cultures of primary human gingival fibroblasts (HGF), periodontal ligament fibroblasts (PDLF) and human osteoblast-like (HOB) cells in vitro. Methods Three commercially available collagen membranes [$ TutoDent^{®} $ (TD), $ Resodont^{®} $ (RD) and $ BioGide^{®} $ (BG)] as well as three nonresorbable polytetrafluoroethylene (PTFE) membranes [ACE (AC), $ Cytoplast^{®} $ (CT) and TefGen-$ FD^{®} $ (TG)] were tested. Cells plated on culture dishes (CD) served as positive controls. The effect of the barrier membranes on HGF, PDLF as well as HOB cells was assessed by the Alamar Blue fluorometric proliferation assay after 1, 2.5, 4, 24 and 48 h time periods. The structural and morphological properties of the membranes were evaluated by scanning electron microscopy (SEM). Results The results showed that of the six barriers tested, TD and RD demonstrated the highest rate of HGF proliferation at both earlier (1 h) and later (48 h) time periods (P < 0.001) compared to all other tested barriers and CD. Similarly, TD, RD and BG had significantly higher numbers of cells at all time periods when compared with the positive control in PDLF culture (P ≤ 0.001). In HOB cell culture, the highest rate of cell proliferation was also calculated for TD at all time periods (P < 0.001). SEM observations demonstrated a microporous structure of all collagen membranes, with a compact top surface and a porous bottom surface, whereas the nonresorbable PTFE membranes demonstrated a homogenous structure with a symmetric dense skin layer. Conclusion Results from the present study suggested that GTR membrane materials, per se, may influence cell proliferation in the process of periodontal tissue/bone regeneration. Among the six membranes examined, the bioabsorbable membranes demonstrated to be more suitable to stimulate cellular proliferation compared to nonresorbable PTFE membranes. Collagen Membrane (dpeaa)DE-He213 Gingival Fibroblast (dpeaa)DE-He213 Human Gingival Fibroblast (dpeaa)DE-He213 Guide Tissue Regeneration (dpeaa)DE-He213 Alamar Blue Assay (dpeaa)DE-He213 Reichert, Christoph aut Götz, Hermann aut Röhrig, Bernd aut Smeets, Ralf aut Willershausen, Brita aut Enthalten in Head & face medicine London : BioMed Central, 2005 4(2008), 1 vom: 14. Okt. (DE-627)499547799 (DE-600)2202219-3 1746-160X nnns volume:4 year:2008 number:1 day:14 month:10 https://dx.doi.org/10.1186/1746-160X-4-22 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 4 2008 1 14 10 |
| spelling |
10.1186/1746-160X-4-22 doi (DE-627)SPR029393949 (SPR)1746-160X-4-22-e DE-627 ger DE-627 rakwb eng Kasaj, Adrian verfasserin aut In vitro evaluation of various bioabsorbable and nonresorbable barrier membranes for guided tissue regeneration 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Kasaj et al; licensee BioMed Central Ltd. 2008 Background Different types of bioabsorbable and nonresorbable membranes have been widely used for guided tissue regeneration (GTR) with its ultimate goal of regenerating lost periodontal structures. The purpose of the present study was to evaluate the biological effects of various bioabsorbable and nonresorbable membranes in cultures of primary human gingival fibroblasts (HGF), periodontal ligament fibroblasts (PDLF) and human osteoblast-like (HOB) cells in vitro. Methods Three commercially available collagen membranes [$ TutoDent^{®} $ (TD), $ Resodont^{®} $ (RD) and $ BioGide^{®} $ (BG)] as well as three nonresorbable polytetrafluoroethylene (PTFE) membranes [ACE (AC), $ Cytoplast^{®} $ (CT) and TefGen-$ FD^{®} $ (TG)] were tested. Cells plated on culture dishes (CD) served as positive controls. The effect of the barrier membranes on HGF, PDLF as well as HOB cells was assessed by the Alamar Blue fluorometric proliferation assay after 1, 2.5, 4, 24 and 48 h time periods. The structural and morphological properties of the membranes were evaluated by scanning electron microscopy (SEM). Results The results showed that of the six barriers tested, TD and RD demonstrated the highest rate of HGF proliferation at both earlier (1 h) and later (48 h) time periods (P < 0.001) compared to all other tested barriers and CD. Similarly, TD, RD and BG had significantly higher numbers of cells at all time periods when compared with the positive control in PDLF culture (P ≤ 0.001). In HOB cell culture, the highest rate of cell proliferation was also calculated for TD at all time periods (P < 0.001). SEM observations demonstrated a microporous structure of all collagen membranes, with a compact top surface and a porous bottom surface, whereas the nonresorbable PTFE membranes demonstrated a homogenous structure with a symmetric dense skin layer. Conclusion Results from the present study suggested that GTR membrane materials, per se, may influence cell proliferation in the process of periodontal tissue/bone regeneration. Among the six membranes examined, the bioabsorbable membranes demonstrated to be more suitable to stimulate cellular proliferation compared to nonresorbable PTFE membranes. Collagen Membrane (dpeaa)DE-He213 Gingival Fibroblast (dpeaa)DE-He213 Human Gingival Fibroblast (dpeaa)DE-He213 Guide Tissue Regeneration (dpeaa)DE-He213 Alamar Blue Assay (dpeaa)DE-He213 Reichert, Christoph aut Götz, Hermann aut Röhrig, Bernd aut Smeets, Ralf aut Willershausen, Brita aut Enthalten in Head & face medicine London : BioMed Central, 2005 4(2008), 1 vom: 14. Okt. (DE-627)499547799 (DE-600)2202219-3 1746-160X nnns volume:4 year:2008 number:1 day:14 month:10 https://dx.doi.org/10.1186/1746-160X-4-22 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 4 2008 1 14 10 |
| allfields_unstemmed |
10.1186/1746-160X-4-22 doi (DE-627)SPR029393949 (SPR)1746-160X-4-22-e DE-627 ger DE-627 rakwb eng Kasaj, Adrian verfasserin aut In vitro evaluation of various bioabsorbable and nonresorbable barrier membranes for guided tissue regeneration 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Kasaj et al; licensee BioMed Central Ltd. 2008 Background Different types of bioabsorbable and nonresorbable membranes have been widely used for guided tissue regeneration (GTR) with its ultimate goal of regenerating lost periodontal structures. The purpose of the present study was to evaluate the biological effects of various bioabsorbable and nonresorbable membranes in cultures of primary human gingival fibroblasts (HGF), periodontal ligament fibroblasts (PDLF) and human osteoblast-like (HOB) cells in vitro. Methods Three commercially available collagen membranes [$ TutoDent^{®} $ (TD), $ Resodont^{®} $ (RD) and $ BioGide^{®} $ (BG)] as well as three nonresorbable polytetrafluoroethylene (PTFE) membranes [ACE (AC), $ Cytoplast^{®} $ (CT) and TefGen-$ FD^{®} $ (TG)] were tested. Cells plated on culture dishes (CD) served as positive controls. The effect of the barrier membranes on HGF, PDLF as well as HOB cells was assessed by the Alamar Blue fluorometric proliferation assay after 1, 2.5, 4, 24 and 48 h time periods. The structural and morphological properties of the membranes were evaluated by scanning electron microscopy (SEM). Results The results showed that of the six barriers tested, TD and RD demonstrated the highest rate of HGF proliferation at both earlier (1 h) and later (48 h) time periods (P < 0.001) compared to all other tested barriers and CD. Similarly, TD, RD and BG had significantly higher numbers of cells at all time periods when compared with the positive control in PDLF culture (P ≤ 0.001). In HOB cell culture, the highest rate of cell proliferation was also calculated for TD at all time periods (P < 0.001). SEM observations demonstrated a microporous structure of all collagen membranes, with a compact top surface and a porous bottom surface, whereas the nonresorbable PTFE membranes demonstrated a homogenous structure with a symmetric dense skin layer. Conclusion Results from the present study suggested that GTR membrane materials, per se, may influence cell proliferation in the process of periodontal tissue/bone regeneration. Among the six membranes examined, the bioabsorbable membranes demonstrated to be more suitable to stimulate cellular proliferation compared to nonresorbable PTFE membranes. Collagen Membrane (dpeaa)DE-He213 Gingival Fibroblast (dpeaa)DE-He213 Human Gingival Fibroblast (dpeaa)DE-He213 Guide Tissue Regeneration (dpeaa)DE-He213 Alamar Blue Assay (dpeaa)DE-He213 Reichert, Christoph aut Götz, Hermann aut Röhrig, Bernd aut Smeets, Ralf aut Willershausen, Brita aut Enthalten in Head & face medicine London : BioMed Central, 2005 4(2008), 1 vom: 14. Okt. (DE-627)499547799 (DE-600)2202219-3 1746-160X nnns volume:4 year:2008 number:1 day:14 month:10 https://dx.doi.org/10.1186/1746-160X-4-22 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 4 2008 1 14 10 |
| allfieldsGer |
10.1186/1746-160X-4-22 doi (DE-627)SPR029393949 (SPR)1746-160X-4-22-e DE-627 ger DE-627 rakwb eng Kasaj, Adrian verfasserin aut In vitro evaluation of various bioabsorbable and nonresorbable barrier membranes for guided tissue regeneration 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Kasaj et al; licensee BioMed Central Ltd. 2008 Background Different types of bioabsorbable and nonresorbable membranes have been widely used for guided tissue regeneration (GTR) with its ultimate goal of regenerating lost periodontal structures. The purpose of the present study was to evaluate the biological effects of various bioabsorbable and nonresorbable membranes in cultures of primary human gingival fibroblasts (HGF), periodontal ligament fibroblasts (PDLF) and human osteoblast-like (HOB) cells in vitro. Methods Three commercially available collagen membranes [$ TutoDent^{®} $ (TD), $ Resodont^{®} $ (RD) and $ BioGide^{®} $ (BG)] as well as three nonresorbable polytetrafluoroethylene (PTFE) membranes [ACE (AC), $ Cytoplast^{®} $ (CT) and TefGen-$ FD^{®} $ (TG)] were tested. Cells plated on culture dishes (CD) served as positive controls. The effect of the barrier membranes on HGF, PDLF as well as HOB cells was assessed by the Alamar Blue fluorometric proliferation assay after 1, 2.5, 4, 24 and 48 h time periods. The structural and morphological properties of the membranes were evaluated by scanning electron microscopy (SEM). Results The results showed that of the six barriers tested, TD and RD demonstrated the highest rate of HGF proliferation at both earlier (1 h) and later (48 h) time periods (P < 0.001) compared to all other tested barriers and CD. Similarly, TD, RD and BG had significantly higher numbers of cells at all time periods when compared with the positive control in PDLF culture (P ≤ 0.001). In HOB cell culture, the highest rate of cell proliferation was also calculated for TD at all time periods (P < 0.001). SEM observations demonstrated a microporous structure of all collagen membranes, with a compact top surface and a porous bottom surface, whereas the nonresorbable PTFE membranes demonstrated a homogenous structure with a symmetric dense skin layer. Conclusion Results from the present study suggested that GTR membrane materials, per se, may influence cell proliferation in the process of periodontal tissue/bone regeneration. Among the six membranes examined, the bioabsorbable membranes demonstrated to be more suitable to stimulate cellular proliferation compared to nonresorbable PTFE membranes. Collagen Membrane (dpeaa)DE-He213 Gingival Fibroblast (dpeaa)DE-He213 Human Gingival Fibroblast (dpeaa)DE-He213 Guide Tissue Regeneration (dpeaa)DE-He213 Alamar Blue Assay (dpeaa)DE-He213 Reichert, Christoph aut Götz, Hermann aut Röhrig, Bernd aut Smeets, Ralf aut Willershausen, Brita aut Enthalten in Head & face medicine London : BioMed Central, 2005 4(2008), 1 vom: 14. Okt. (DE-627)499547799 (DE-600)2202219-3 1746-160X nnns volume:4 year:2008 number:1 day:14 month:10 https://dx.doi.org/10.1186/1746-160X-4-22 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 4 2008 1 14 10 |
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10.1186/1746-160X-4-22 doi (DE-627)SPR029393949 (SPR)1746-160X-4-22-e DE-627 ger DE-627 rakwb eng Kasaj, Adrian verfasserin aut In vitro evaluation of various bioabsorbable and nonresorbable barrier membranes for guided tissue regeneration 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Kasaj et al; licensee BioMed Central Ltd. 2008 Background Different types of bioabsorbable and nonresorbable membranes have been widely used for guided tissue regeneration (GTR) with its ultimate goal of regenerating lost periodontal structures. The purpose of the present study was to evaluate the biological effects of various bioabsorbable and nonresorbable membranes in cultures of primary human gingival fibroblasts (HGF), periodontal ligament fibroblasts (PDLF) and human osteoblast-like (HOB) cells in vitro. Methods Three commercially available collagen membranes [$ TutoDent^{®} $ (TD), $ Resodont^{®} $ (RD) and $ BioGide^{®} $ (BG)] as well as three nonresorbable polytetrafluoroethylene (PTFE) membranes [ACE (AC), $ Cytoplast^{®} $ (CT) and TefGen-$ FD^{®} $ (TG)] were tested. Cells plated on culture dishes (CD) served as positive controls. The effect of the barrier membranes on HGF, PDLF as well as HOB cells was assessed by the Alamar Blue fluorometric proliferation assay after 1, 2.5, 4, 24 and 48 h time periods. The structural and morphological properties of the membranes were evaluated by scanning electron microscopy (SEM). Results The results showed that of the six barriers tested, TD and RD demonstrated the highest rate of HGF proliferation at both earlier (1 h) and later (48 h) time periods (P < 0.001) compared to all other tested barriers and CD. Similarly, TD, RD and BG had significantly higher numbers of cells at all time periods when compared with the positive control in PDLF culture (P ≤ 0.001). In HOB cell culture, the highest rate of cell proliferation was also calculated for TD at all time periods (P < 0.001). SEM observations demonstrated a microporous structure of all collagen membranes, with a compact top surface and a porous bottom surface, whereas the nonresorbable PTFE membranes demonstrated a homogenous structure with a symmetric dense skin layer. Conclusion Results from the present study suggested that GTR membrane materials, per se, may influence cell proliferation in the process of periodontal tissue/bone regeneration. Among the six membranes examined, the bioabsorbable membranes demonstrated to be more suitable to stimulate cellular proliferation compared to nonresorbable PTFE membranes. Collagen Membrane (dpeaa)DE-He213 Gingival Fibroblast (dpeaa)DE-He213 Human Gingival Fibroblast (dpeaa)DE-He213 Guide Tissue Regeneration (dpeaa)DE-He213 Alamar Blue Assay (dpeaa)DE-He213 Reichert, Christoph aut Götz, Hermann aut Röhrig, Bernd aut Smeets, Ralf aut Willershausen, Brita aut Enthalten in Head & face medicine London : BioMed Central, 2005 4(2008), 1 vom: 14. Okt. (DE-627)499547799 (DE-600)2202219-3 1746-160X nnns volume:4 year:2008 number:1 day:14 month:10 https://dx.doi.org/10.1186/1746-160X-4-22 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 4 2008 1 14 10 |
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In vitro evaluation of various bioabsorbable and nonresorbable barrier membranes for guided tissue regeneration |
| abstract |
Background Different types of bioabsorbable and nonresorbable membranes have been widely used for guided tissue regeneration (GTR) with its ultimate goal of regenerating lost periodontal structures. The purpose of the present study was to evaluate the biological effects of various bioabsorbable and nonresorbable membranes in cultures of primary human gingival fibroblasts (HGF), periodontal ligament fibroblasts (PDLF) and human osteoblast-like (HOB) cells in vitro. Methods Three commercially available collagen membranes [$ TutoDent^{®} $ (TD), $ Resodont^{®} $ (RD) and $ BioGide^{®} $ (BG)] as well as three nonresorbable polytetrafluoroethylene (PTFE) membranes [ACE (AC), $ Cytoplast^{®} $ (CT) and TefGen-$ FD^{®} $ (TG)] were tested. Cells plated on culture dishes (CD) served as positive controls. The effect of the barrier membranes on HGF, PDLF as well as HOB cells was assessed by the Alamar Blue fluorometric proliferation assay after 1, 2.5, 4, 24 and 48 h time periods. The structural and morphological properties of the membranes were evaluated by scanning electron microscopy (SEM). Results The results showed that of the six barriers tested, TD and RD demonstrated the highest rate of HGF proliferation at both earlier (1 h) and later (48 h) time periods (P < 0.001) compared to all other tested barriers and CD. Similarly, TD, RD and BG had significantly higher numbers of cells at all time periods when compared with the positive control in PDLF culture (P ≤ 0.001). In HOB cell culture, the highest rate of cell proliferation was also calculated for TD at all time periods (P < 0.001). SEM observations demonstrated a microporous structure of all collagen membranes, with a compact top surface and a porous bottom surface, whereas the nonresorbable PTFE membranes demonstrated a homogenous structure with a symmetric dense skin layer. Conclusion Results from the present study suggested that GTR membrane materials, per se, may influence cell proliferation in the process of periodontal tissue/bone regeneration. Among the six membranes examined, the bioabsorbable membranes demonstrated to be more suitable to stimulate cellular proliferation compared to nonresorbable PTFE membranes. © Kasaj et al; licensee BioMed Central Ltd. 2008 |
| abstractGer |
Background Different types of bioabsorbable and nonresorbable membranes have been widely used for guided tissue regeneration (GTR) with its ultimate goal of regenerating lost periodontal structures. The purpose of the present study was to evaluate the biological effects of various bioabsorbable and nonresorbable membranes in cultures of primary human gingival fibroblasts (HGF), periodontal ligament fibroblasts (PDLF) and human osteoblast-like (HOB) cells in vitro. Methods Three commercially available collagen membranes [$ TutoDent^{®} $ (TD), $ Resodont^{®} $ (RD) and $ BioGide^{®} $ (BG)] as well as three nonresorbable polytetrafluoroethylene (PTFE) membranes [ACE (AC), $ Cytoplast^{®} $ (CT) and TefGen-$ FD^{®} $ (TG)] were tested. Cells plated on culture dishes (CD) served as positive controls. The effect of the barrier membranes on HGF, PDLF as well as HOB cells was assessed by the Alamar Blue fluorometric proliferation assay after 1, 2.5, 4, 24 and 48 h time periods. The structural and morphological properties of the membranes were evaluated by scanning electron microscopy (SEM). Results The results showed that of the six barriers tested, TD and RD demonstrated the highest rate of HGF proliferation at both earlier (1 h) and later (48 h) time periods (P < 0.001) compared to all other tested barriers and CD. Similarly, TD, RD and BG had significantly higher numbers of cells at all time periods when compared with the positive control in PDLF culture (P ≤ 0.001). In HOB cell culture, the highest rate of cell proliferation was also calculated for TD at all time periods (P < 0.001). SEM observations demonstrated a microporous structure of all collagen membranes, with a compact top surface and a porous bottom surface, whereas the nonresorbable PTFE membranes demonstrated a homogenous structure with a symmetric dense skin layer. Conclusion Results from the present study suggested that GTR membrane materials, per se, may influence cell proliferation in the process of periodontal tissue/bone regeneration. Among the six membranes examined, the bioabsorbable membranes demonstrated to be more suitable to stimulate cellular proliferation compared to nonresorbable PTFE membranes. © Kasaj et al; licensee BioMed Central Ltd. 2008 |
| abstract_unstemmed |
Background Different types of bioabsorbable and nonresorbable membranes have been widely used for guided tissue regeneration (GTR) with its ultimate goal of regenerating lost periodontal structures. The purpose of the present study was to evaluate the biological effects of various bioabsorbable and nonresorbable membranes in cultures of primary human gingival fibroblasts (HGF), periodontal ligament fibroblasts (PDLF) and human osteoblast-like (HOB) cells in vitro. Methods Three commercially available collagen membranes [$ TutoDent^{®} $ (TD), $ Resodont^{®} $ (RD) and $ BioGide^{®} $ (BG)] as well as three nonresorbable polytetrafluoroethylene (PTFE) membranes [ACE (AC), $ Cytoplast^{®} $ (CT) and TefGen-$ FD^{®} $ (TG)] were tested. Cells plated on culture dishes (CD) served as positive controls. The effect of the barrier membranes on HGF, PDLF as well as HOB cells was assessed by the Alamar Blue fluorometric proliferation assay after 1, 2.5, 4, 24 and 48 h time periods. The structural and morphological properties of the membranes were evaluated by scanning electron microscopy (SEM). Results The results showed that of the six barriers tested, TD and RD demonstrated the highest rate of HGF proliferation at both earlier (1 h) and later (48 h) time periods (P < 0.001) compared to all other tested barriers and CD. Similarly, TD, RD and BG had significantly higher numbers of cells at all time periods when compared with the positive control in PDLF culture (P ≤ 0.001). In HOB cell culture, the highest rate of cell proliferation was also calculated for TD at all time periods (P < 0.001). SEM observations demonstrated a microporous structure of all collagen membranes, with a compact top surface and a porous bottom surface, whereas the nonresorbable PTFE membranes demonstrated a homogenous structure with a symmetric dense skin layer. Conclusion Results from the present study suggested that GTR membrane materials, per se, may influence cell proliferation in the process of periodontal tissue/bone regeneration. Among the six membranes examined, the bioabsorbable membranes demonstrated to be more suitable to stimulate cellular proliferation compared to nonresorbable PTFE membranes. © Kasaj et al; licensee BioMed Central Ltd. 2008 |
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| title_short |
In vitro evaluation of various bioabsorbable and nonresorbable barrier membranes for guided tissue regeneration |
| url |
https://dx.doi.org/10.1186/1746-160X-4-22 |
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| author2 |
Reichert, Christoph Götz, Hermann Röhrig, Bernd Smeets, Ralf Willershausen, Brita |
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