Intercellular Adhesive Structures Between Stellate Cells – An Analysis in Cultured Human Hepatic Stellate Cells
Abstract To investigate whether or not hepatic stellate cells can form intercellular junctions with each other, we cultured human stellate cells (LI90) on different kinds of substrata. Intercellular junctions were detected between these cultured stellate cells by transmission electron microscopy (TE...
Ausführliche Beschreibung
Autor*in: |
Imai, Katsuyuki [verfasserIn] |
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E-Artikel |
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Englisch |
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2004 |
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Anmerkung: |
© Imai et al; licensee BioMed Central Ltd 2004. This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
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Übergeordnetes Werk: |
Enthalten in: Comparative hepatology - [S.l.] : BioMed Central, 2002, 3(2004), Suppl 1 vom: 14. Jan. |
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Übergeordnetes Werk: |
volume:3 ; year:2004 ; number:Suppl 1 ; day:14 ; month:01 |
Links: |
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DOI / URN: |
10.1186/1476-5926-2-S1-S13 |
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Katalog-ID: |
SPR029397316 |
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520 | |a Abstract To investigate whether or not hepatic stellate cells can form intercellular junctions with each other, we cultured human stellate cells (LI90) on different kinds of substrata. Intercellular junctions were detected between these cultured stellate cells by transmission electron microscopy (TEM). The molecular components of the intercellular adhesive structures were identified by immunofluorescence microscopy. Immunofluorescence for cadherin and catenins was detected at the adhesion sites between the cultured stellate cells. Thus, the intercellular junctions were indicated to be adherens junctions at the molecular level. The junctions developed in the cultured stellate cells irrespective of the type of substratum. These data suggest that the junctional formation between the stellate cells occurs in vivo as well as in vitro. | ||
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700 | 1 | |a Senoo, Haruki |4 aut | |
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10.1186/1476-5926-2-S1-S13 doi (DE-627)SPR029397316 (SPR)1476-5926-2-S1-S13-e DE-627 ger DE-627 rakwb eng Imai, Katsuyuki verfasserin aut Intercellular Adhesive Structures Between Stellate Cells – An Analysis in Cultured Human Hepatic Stellate Cells 2004 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Imai et al; licensee BioMed Central Ltd 2004. This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract To investigate whether or not hepatic stellate cells can form intercellular junctions with each other, we cultured human stellate cells (LI90) on different kinds of substrata. Intercellular junctions were detected between these cultured stellate cells by transmission electron microscopy (TEM). The molecular components of the intercellular adhesive structures were identified by immunofluorescence microscopy. Immunofluorescence for cadherin and catenins was detected at the adhesion sites between the cultured stellate cells. Thus, the intercellular junctions were indicated to be adherens junctions at the molecular level. The junctions developed in the cultured stellate cells irrespective of the type of substratum. These data suggest that the junctional formation between the stellate cells occurs in vivo as well as in vitro. Stellate Cell (dpeaa)DE-He213 Hepatic Stellate Cell (dpeaa)DE-He213 Immunofluorescence Microscopy (dpeaa)DE-He213 Adherens Junction (dpeaa)DE-He213 Molecular Component (dpeaa)DE-He213 Sato, Mitsuru aut Sato, Takeya aut Kojima, Naosuke aut Miura, Mitsutaka aut Higashi, Nobuyo aut Wang, Da-Ren aut Suzuki, Shinsuke aut Senoo, Haruki aut Enthalten in Comparative hepatology [S.l.] : BioMed Central, 2002 3(2004), Suppl 1 vom: 14. Jan. (DE-627)355684209 (DE-600)2091772-7 1476-5926 nnns volume:3 year:2004 number:Suppl 1 day:14 month:01 https://dx.doi.org/10.1186/1476-5926-2-S1-S13 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 3 2004 Suppl 1 14 01 |
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10.1186/1476-5926-2-S1-S13 doi (DE-627)SPR029397316 (SPR)1476-5926-2-S1-S13-e DE-627 ger DE-627 rakwb eng Imai, Katsuyuki verfasserin aut Intercellular Adhesive Structures Between Stellate Cells – An Analysis in Cultured Human Hepatic Stellate Cells 2004 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Imai et al; licensee BioMed Central Ltd 2004. This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract To investigate whether or not hepatic stellate cells can form intercellular junctions with each other, we cultured human stellate cells (LI90) on different kinds of substrata. Intercellular junctions were detected between these cultured stellate cells by transmission electron microscopy (TEM). The molecular components of the intercellular adhesive structures were identified by immunofluorescence microscopy. Immunofluorescence for cadherin and catenins was detected at the adhesion sites between the cultured stellate cells. Thus, the intercellular junctions were indicated to be adherens junctions at the molecular level. The junctions developed in the cultured stellate cells irrespective of the type of substratum. These data suggest that the junctional formation between the stellate cells occurs in vivo as well as in vitro. Stellate Cell (dpeaa)DE-He213 Hepatic Stellate Cell (dpeaa)DE-He213 Immunofluorescence Microscopy (dpeaa)DE-He213 Adherens Junction (dpeaa)DE-He213 Molecular Component (dpeaa)DE-He213 Sato, Mitsuru aut Sato, Takeya aut Kojima, Naosuke aut Miura, Mitsutaka aut Higashi, Nobuyo aut Wang, Da-Ren aut Suzuki, Shinsuke aut Senoo, Haruki aut Enthalten in Comparative hepatology [S.l.] : BioMed Central, 2002 3(2004), Suppl 1 vom: 14. Jan. (DE-627)355684209 (DE-600)2091772-7 1476-5926 nnns volume:3 year:2004 number:Suppl 1 day:14 month:01 https://dx.doi.org/10.1186/1476-5926-2-S1-S13 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 3 2004 Suppl 1 14 01 |
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10.1186/1476-5926-2-S1-S13 doi (DE-627)SPR029397316 (SPR)1476-5926-2-S1-S13-e DE-627 ger DE-627 rakwb eng Imai, Katsuyuki verfasserin aut Intercellular Adhesive Structures Between Stellate Cells – An Analysis in Cultured Human Hepatic Stellate Cells 2004 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Imai et al; licensee BioMed Central Ltd 2004. This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract To investigate whether or not hepatic stellate cells can form intercellular junctions with each other, we cultured human stellate cells (LI90) on different kinds of substrata. Intercellular junctions were detected between these cultured stellate cells by transmission electron microscopy (TEM). The molecular components of the intercellular adhesive structures were identified by immunofluorescence microscopy. Immunofluorescence for cadherin and catenins was detected at the adhesion sites between the cultured stellate cells. Thus, the intercellular junctions were indicated to be adherens junctions at the molecular level. The junctions developed in the cultured stellate cells irrespective of the type of substratum. These data suggest that the junctional formation between the stellate cells occurs in vivo as well as in vitro. Stellate Cell (dpeaa)DE-He213 Hepatic Stellate Cell (dpeaa)DE-He213 Immunofluorescence Microscopy (dpeaa)DE-He213 Adherens Junction (dpeaa)DE-He213 Molecular Component (dpeaa)DE-He213 Sato, Mitsuru aut Sato, Takeya aut Kojima, Naosuke aut Miura, Mitsutaka aut Higashi, Nobuyo aut Wang, Da-Ren aut Suzuki, Shinsuke aut Senoo, Haruki aut Enthalten in Comparative hepatology [S.l.] : BioMed Central, 2002 3(2004), Suppl 1 vom: 14. Jan. (DE-627)355684209 (DE-600)2091772-7 1476-5926 nnns volume:3 year:2004 number:Suppl 1 day:14 month:01 https://dx.doi.org/10.1186/1476-5926-2-S1-S13 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 3 2004 Suppl 1 14 01 |
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10.1186/1476-5926-2-S1-S13 doi (DE-627)SPR029397316 (SPR)1476-5926-2-S1-S13-e DE-627 ger DE-627 rakwb eng Imai, Katsuyuki verfasserin aut Intercellular Adhesive Structures Between Stellate Cells – An Analysis in Cultured Human Hepatic Stellate Cells 2004 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Imai et al; licensee BioMed Central Ltd 2004. This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract To investigate whether or not hepatic stellate cells can form intercellular junctions with each other, we cultured human stellate cells (LI90) on different kinds of substrata. Intercellular junctions were detected between these cultured stellate cells by transmission electron microscopy (TEM). The molecular components of the intercellular adhesive structures were identified by immunofluorescence microscopy. Immunofluorescence for cadherin and catenins was detected at the adhesion sites between the cultured stellate cells. Thus, the intercellular junctions were indicated to be adherens junctions at the molecular level. The junctions developed in the cultured stellate cells irrespective of the type of substratum. These data suggest that the junctional formation between the stellate cells occurs in vivo as well as in vitro. Stellate Cell (dpeaa)DE-He213 Hepatic Stellate Cell (dpeaa)DE-He213 Immunofluorescence Microscopy (dpeaa)DE-He213 Adherens Junction (dpeaa)DE-He213 Molecular Component (dpeaa)DE-He213 Sato, Mitsuru aut Sato, Takeya aut Kojima, Naosuke aut Miura, Mitsutaka aut Higashi, Nobuyo aut Wang, Da-Ren aut Suzuki, Shinsuke aut Senoo, Haruki aut Enthalten in Comparative hepatology [S.l.] : BioMed Central, 2002 3(2004), Suppl 1 vom: 14. Jan. (DE-627)355684209 (DE-600)2091772-7 1476-5926 nnns volume:3 year:2004 number:Suppl 1 day:14 month:01 https://dx.doi.org/10.1186/1476-5926-2-S1-S13 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 3 2004 Suppl 1 14 01 |
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10.1186/1476-5926-2-S1-S13 doi (DE-627)SPR029397316 (SPR)1476-5926-2-S1-S13-e DE-627 ger DE-627 rakwb eng Imai, Katsuyuki verfasserin aut Intercellular Adhesive Structures Between Stellate Cells – An Analysis in Cultured Human Hepatic Stellate Cells 2004 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Imai et al; licensee BioMed Central Ltd 2004. This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract To investigate whether or not hepatic stellate cells can form intercellular junctions with each other, we cultured human stellate cells (LI90) on different kinds of substrata. Intercellular junctions were detected between these cultured stellate cells by transmission electron microscopy (TEM). The molecular components of the intercellular adhesive structures were identified by immunofluorescence microscopy. Immunofluorescence for cadherin and catenins was detected at the adhesion sites between the cultured stellate cells. Thus, the intercellular junctions were indicated to be adherens junctions at the molecular level. The junctions developed in the cultured stellate cells irrespective of the type of substratum. These data suggest that the junctional formation between the stellate cells occurs in vivo as well as in vitro. Stellate Cell (dpeaa)DE-He213 Hepatic Stellate Cell (dpeaa)DE-He213 Immunofluorescence Microscopy (dpeaa)DE-He213 Adherens Junction (dpeaa)DE-He213 Molecular Component (dpeaa)DE-He213 Sato, Mitsuru aut Sato, Takeya aut Kojima, Naosuke aut Miura, Mitsutaka aut Higashi, Nobuyo aut Wang, Da-Ren aut Suzuki, Shinsuke aut Senoo, Haruki aut Enthalten in Comparative hepatology [S.l.] : BioMed Central, 2002 3(2004), Suppl 1 vom: 14. Jan. (DE-627)355684209 (DE-600)2091772-7 1476-5926 nnns volume:3 year:2004 number:Suppl 1 day:14 month:01 https://dx.doi.org/10.1186/1476-5926-2-S1-S13 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 3 2004 Suppl 1 14 01 |
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intercellular adhesive structures between stellate cells – an analysis in cultured human hepatic stellate cells |
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Intercellular Adhesive Structures Between Stellate Cells – An Analysis in Cultured Human Hepatic Stellate Cells |
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Abstract To investigate whether or not hepatic stellate cells can form intercellular junctions with each other, we cultured human stellate cells (LI90) on different kinds of substrata. Intercellular junctions were detected between these cultured stellate cells by transmission electron microscopy (TEM). The molecular components of the intercellular adhesive structures were identified by immunofluorescence microscopy. Immunofluorescence for cadherin and catenins was detected at the adhesion sites between the cultured stellate cells. Thus, the intercellular junctions were indicated to be adherens junctions at the molecular level. The junctions developed in the cultured stellate cells irrespective of the type of substratum. These data suggest that the junctional formation between the stellate cells occurs in vivo as well as in vitro. © Imai et al; licensee BioMed Central Ltd 2004. This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
abstractGer |
Abstract To investigate whether or not hepatic stellate cells can form intercellular junctions with each other, we cultured human stellate cells (LI90) on different kinds of substrata. Intercellular junctions were detected between these cultured stellate cells by transmission electron microscopy (TEM). The molecular components of the intercellular adhesive structures were identified by immunofluorescence microscopy. Immunofluorescence for cadherin and catenins was detected at the adhesion sites between the cultured stellate cells. Thus, the intercellular junctions were indicated to be adherens junctions at the molecular level. The junctions developed in the cultured stellate cells irrespective of the type of substratum. These data suggest that the junctional formation between the stellate cells occurs in vivo as well as in vitro. © Imai et al; licensee BioMed Central Ltd 2004. This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
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Abstract To investigate whether or not hepatic stellate cells can form intercellular junctions with each other, we cultured human stellate cells (LI90) on different kinds of substrata. Intercellular junctions were detected between these cultured stellate cells by transmission electron microscopy (TEM). The molecular components of the intercellular adhesive structures were identified by immunofluorescence microscopy. Immunofluorescence for cadherin and catenins was detected at the adhesion sites between the cultured stellate cells. Thus, the intercellular junctions were indicated to be adherens junctions at the molecular level. The junctions developed in the cultured stellate cells irrespective of the type of substratum. These data suggest that the junctional formation between the stellate cells occurs in vivo as well as in vitro. © Imai et al; licensee BioMed Central Ltd 2004. This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
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