Utilization of computer vision and multispectral imaging techniques for classification of cowpea (Vigna unguiculata) seeds
Background The traditional methods for evaluating seeds are usually performed through destructive sampling followed by physical, physiological, biochemical and molecular determinations. Whilst proven to be effective, these approaches can be criticized as being destructive, time consuming, labor inte...
Ausführliche Beschreibung
Autor*in: |
ElMasry, Gamal [verfasserIn] |
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Englisch |
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2019 |
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© The Author(s) 2019 |
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Übergeordnetes Werk: |
Enthalten in: Plant methods - London : BioMed Central, 2005, 15(2019), 1 vom: 12. März |
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Übergeordnetes Werk: |
volume:15 ; year:2019 ; number:1 ; day:12 ; month:03 |
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DOI / URN: |
10.1186/s13007-019-0411-2 |
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SPR029411025 |
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520 | |a Background The traditional methods for evaluating seeds are usually performed through destructive sampling followed by physical, physiological, biochemical and molecular determinations. Whilst proven to be effective, these approaches can be criticized as being destructive, time consuming, labor intensive and requiring experienced seed analysts. Thus, the objective of this study was to investigate the potential of computer vision and multispectral imaging systems supported with multivariate analysis for high-throughput classification of cowpea (Vigna unguiculata) seeds. An automated computer-vision germination system was utilized for uninterrupted monitoring of seeds during imbibition and germination to identify different categories of all individual seeds. By using spectral signatures of single cowpea seeds extracted from multispectral images, different multivariate analysis models based on linear discriminant analysis (LDA) were developed for classifying the seeds into different categories according to ageing, viability, seedling condition and speed of germination. Results The results revealed that the LDA models had good accuracy in distinguishing ‘Aged’ and ‘Non-aged’ seeds with an overall correct classification (OCC) of 97.51, 96.76 and 97%, ‘Germinated’ and ‘Non-germinated’ seeds with OCC of 81.80, 79.05 and 81.0%, ‘Early germinated’, ‘Medium germinated’ and ‘Dead’ seeds with OCC of 77.21, 74.93 and 68.00% and among seeds that give ‘Normal’ and ‘Abnormal’ seedlings with OCC of 68.08, 64.34 and 62.00% in training, cross-validation and independent validation data sets, respectively. Image processing routines were also developed to exploit the full power of the multispectral imaging system in visualizing the difference among seed categories by applying the discriminant model in a pixel-wise manner. Conclusion The results demonstrated the capability of the multispectral imaging system in the ultraviolet, visible and shortwave near infrared range to provide the required information necessary for the discrimination of individual cowpea seeds to different classes. Considering the short time of image acquisition and limited sample preparation, this stat-of-the art multispectral imaging method and chemometric analysis in classifying seeds could be a valuable tool for on-line classification protocols in cost-effective real-time sorting and grading processes as it provides not only morphological and physical features but also chemical information for the seeds being examined. Implementing image processing algorithms specific for seed quality assessment along with the declining cost and increasing power of computer hardware is very efficient to make the development of such computer-integrated systems more attractive in automatic inspection of seed quality. | ||
650 | 4 | |a Cowpea seeds |7 (dpeaa)DE-He213 | |
650 | 4 | |a Black-eyed seeds |7 (dpeaa)DE-He213 | |
650 | 4 | |a Plant development |7 (dpeaa)DE-He213 | |
650 | 4 | |a Germination |7 (dpeaa)DE-He213 | |
650 | 4 | |a Phenotyping |7 (dpeaa)DE-He213 | |
650 | 4 | |a Chemometric |7 (dpeaa)DE-He213 | |
650 | 4 | |a Multispectral imaging |7 (dpeaa)DE-He213 | |
700 | 1 | |a Mandour, Nasser |4 aut | |
700 | 1 | |a Wagner, Marie-Hélène |4 aut | |
700 | 1 | |a Demilly, Didier |4 aut | |
700 | 1 | |a Verdier, Jerome |4 aut | |
700 | 1 | |a Belin, Etienne |4 aut | |
700 | 1 | |a Rousseau, David |4 aut | |
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10.1186/s13007-019-0411-2 doi (DE-627)SPR029411025 (SPR)s13007-019-0411-2-e DE-627 ger DE-627 rakwb eng ElMasry, Gamal verfasserin aut Utilization of computer vision and multispectral imaging techniques for classification of cowpea (Vigna unguiculata) seeds 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2019 Background The traditional methods for evaluating seeds are usually performed through destructive sampling followed by physical, physiological, biochemical and molecular determinations. Whilst proven to be effective, these approaches can be criticized as being destructive, time consuming, labor intensive and requiring experienced seed analysts. Thus, the objective of this study was to investigate the potential of computer vision and multispectral imaging systems supported with multivariate analysis for high-throughput classification of cowpea (Vigna unguiculata) seeds. An automated computer-vision germination system was utilized for uninterrupted monitoring of seeds during imbibition and germination to identify different categories of all individual seeds. By using spectral signatures of single cowpea seeds extracted from multispectral images, different multivariate analysis models based on linear discriminant analysis (LDA) were developed for classifying the seeds into different categories according to ageing, viability, seedling condition and speed of germination. Results The results revealed that the LDA models had good accuracy in distinguishing ‘Aged’ and ‘Non-aged’ seeds with an overall correct classification (OCC) of 97.51, 96.76 and 97%, ‘Germinated’ and ‘Non-germinated’ seeds with OCC of 81.80, 79.05 and 81.0%, ‘Early germinated’, ‘Medium germinated’ and ‘Dead’ seeds with OCC of 77.21, 74.93 and 68.00% and among seeds that give ‘Normal’ and ‘Abnormal’ seedlings with OCC of 68.08, 64.34 and 62.00% in training, cross-validation and independent validation data sets, respectively. Image processing routines were also developed to exploit the full power of the multispectral imaging system in visualizing the difference among seed categories by applying the discriminant model in a pixel-wise manner. Conclusion The results demonstrated the capability of the multispectral imaging system in the ultraviolet, visible and shortwave near infrared range to provide the required information necessary for the discrimination of individual cowpea seeds to different classes. Considering the short time of image acquisition and limited sample preparation, this stat-of-the art multispectral imaging method and chemometric analysis in classifying seeds could be a valuable tool for on-line classification protocols in cost-effective real-time sorting and grading processes as it provides not only morphological and physical features but also chemical information for the seeds being examined. Implementing image processing algorithms specific for seed quality assessment along with the declining cost and increasing power of computer hardware is very efficient to make the development of such computer-integrated systems more attractive in automatic inspection of seed quality. Cowpea seeds (dpeaa)DE-He213 Black-eyed seeds (dpeaa)DE-He213 Plant development (dpeaa)DE-He213 Germination (dpeaa)DE-He213 Phenotyping (dpeaa)DE-He213 Chemometric (dpeaa)DE-He213 Multispectral imaging (dpeaa)DE-He213 Mandour, Nasser aut Wagner, Marie-Hélène aut Demilly, Didier aut Verdier, Jerome aut Belin, Etienne aut Rousseau, David aut Enthalten in Plant methods London : BioMed Central, 2005 15(2019), 1 vom: 12. März (DE-627)500321191 (DE-600)2203723-8 1746-4811 nnns volume:15 year:2019 number:1 day:12 month:03 https://dx.doi.org/10.1186/s13007-019-0411-2 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2019 1 12 03 |
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10.1186/s13007-019-0411-2 doi (DE-627)SPR029411025 (SPR)s13007-019-0411-2-e DE-627 ger DE-627 rakwb eng ElMasry, Gamal verfasserin aut Utilization of computer vision and multispectral imaging techniques for classification of cowpea (Vigna unguiculata) seeds 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2019 Background The traditional methods for evaluating seeds are usually performed through destructive sampling followed by physical, physiological, biochemical and molecular determinations. Whilst proven to be effective, these approaches can be criticized as being destructive, time consuming, labor intensive and requiring experienced seed analysts. Thus, the objective of this study was to investigate the potential of computer vision and multispectral imaging systems supported with multivariate analysis for high-throughput classification of cowpea (Vigna unguiculata) seeds. An automated computer-vision germination system was utilized for uninterrupted monitoring of seeds during imbibition and germination to identify different categories of all individual seeds. By using spectral signatures of single cowpea seeds extracted from multispectral images, different multivariate analysis models based on linear discriminant analysis (LDA) were developed for classifying the seeds into different categories according to ageing, viability, seedling condition and speed of germination. Results The results revealed that the LDA models had good accuracy in distinguishing ‘Aged’ and ‘Non-aged’ seeds with an overall correct classification (OCC) of 97.51, 96.76 and 97%, ‘Germinated’ and ‘Non-germinated’ seeds with OCC of 81.80, 79.05 and 81.0%, ‘Early germinated’, ‘Medium germinated’ and ‘Dead’ seeds with OCC of 77.21, 74.93 and 68.00% and among seeds that give ‘Normal’ and ‘Abnormal’ seedlings with OCC of 68.08, 64.34 and 62.00% in training, cross-validation and independent validation data sets, respectively. Image processing routines were also developed to exploit the full power of the multispectral imaging system in visualizing the difference among seed categories by applying the discriminant model in a pixel-wise manner. Conclusion The results demonstrated the capability of the multispectral imaging system in the ultraviolet, visible and shortwave near infrared range to provide the required information necessary for the discrimination of individual cowpea seeds to different classes. Considering the short time of image acquisition and limited sample preparation, this stat-of-the art multispectral imaging method and chemometric analysis in classifying seeds could be a valuable tool for on-line classification protocols in cost-effective real-time sorting and grading processes as it provides not only morphological and physical features but also chemical information for the seeds being examined. Implementing image processing algorithms specific for seed quality assessment along with the declining cost and increasing power of computer hardware is very efficient to make the development of such computer-integrated systems more attractive in automatic inspection of seed quality. Cowpea seeds (dpeaa)DE-He213 Black-eyed seeds (dpeaa)DE-He213 Plant development (dpeaa)DE-He213 Germination (dpeaa)DE-He213 Phenotyping (dpeaa)DE-He213 Chemometric (dpeaa)DE-He213 Multispectral imaging (dpeaa)DE-He213 Mandour, Nasser aut Wagner, Marie-Hélène aut Demilly, Didier aut Verdier, Jerome aut Belin, Etienne aut Rousseau, David aut Enthalten in Plant methods London : BioMed Central, 2005 15(2019), 1 vom: 12. März (DE-627)500321191 (DE-600)2203723-8 1746-4811 nnns volume:15 year:2019 number:1 day:12 month:03 https://dx.doi.org/10.1186/s13007-019-0411-2 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2019 1 12 03 |
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10.1186/s13007-019-0411-2 doi (DE-627)SPR029411025 (SPR)s13007-019-0411-2-e DE-627 ger DE-627 rakwb eng ElMasry, Gamal verfasserin aut Utilization of computer vision and multispectral imaging techniques for classification of cowpea (Vigna unguiculata) seeds 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2019 Background The traditional methods for evaluating seeds are usually performed through destructive sampling followed by physical, physiological, biochemical and molecular determinations. Whilst proven to be effective, these approaches can be criticized as being destructive, time consuming, labor intensive and requiring experienced seed analysts. Thus, the objective of this study was to investigate the potential of computer vision and multispectral imaging systems supported with multivariate analysis for high-throughput classification of cowpea (Vigna unguiculata) seeds. An automated computer-vision germination system was utilized for uninterrupted monitoring of seeds during imbibition and germination to identify different categories of all individual seeds. By using spectral signatures of single cowpea seeds extracted from multispectral images, different multivariate analysis models based on linear discriminant analysis (LDA) were developed for classifying the seeds into different categories according to ageing, viability, seedling condition and speed of germination. Results The results revealed that the LDA models had good accuracy in distinguishing ‘Aged’ and ‘Non-aged’ seeds with an overall correct classification (OCC) of 97.51, 96.76 and 97%, ‘Germinated’ and ‘Non-germinated’ seeds with OCC of 81.80, 79.05 and 81.0%, ‘Early germinated’, ‘Medium germinated’ and ‘Dead’ seeds with OCC of 77.21, 74.93 and 68.00% and among seeds that give ‘Normal’ and ‘Abnormal’ seedlings with OCC of 68.08, 64.34 and 62.00% in training, cross-validation and independent validation data sets, respectively. Image processing routines were also developed to exploit the full power of the multispectral imaging system in visualizing the difference among seed categories by applying the discriminant model in a pixel-wise manner. Conclusion The results demonstrated the capability of the multispectral imaging system in the ultraviolet, visible and shortwave near infrared range to provide the required information necessary for the discrimination of individual cowpea seeds to different classes. Considering the short time of image acquisition and limited sample preparation, this stat-of-the art multispectral imaging method and chemometric analysis in classifying seeds could be a valuable tool for on-line classification protocols in cost-effective real-time sorting and grading processes as it provides not only morphological and physical features but also chemical information for the seeds being examined. Implementing image processing algorithms specific for seed quality assessment along with the declining cost and increasing power of computer hardware is very efficient to make the development of such computer-integrated systems more attractive in automatic inspection of seed quality. Cowpea seeds (dpeaa)DE-He213 Black-eyed seeds (dpeaa)DE-He213 Plant development (dpeaa)DE-He213 Germination (dpeaa)DE-He213 Phenotyping (dpeaa)DE-He213 Chemometric (dpeaa)DE-He213 Multispectral imaging (dpeaa)DE-He213 Mandour, Nasser aut Wagner, Marie-Hélène aut Demilly, Didier aut Verdier, Jerome aut Belin, Etienne aut Rousseau, David aut Enthalten in Plant methods London : BioMed Central, 2005 15(2019), 1 vom: 12. März (DE-627)500321191 (DE-600)2203723-8 1746-4811 nnns volume:15 year:2019 number:1 day:12 month:03 https://dx.doi.org/10.1186/s13007-019-0411-2 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2019 1 12 03 |
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10.1186/s13007-019-0411-2 doi (DE-627)SPR029411025 (SPR)s13007-019-0411-2-e DE-627 ger DE-627 rakwb eng ElMasry, Gamal verfasserin aut Utilization of computer vision and multispectral imaging techniques for classification of cowpea (Vigna unguiculata) seeds 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2019 Background The traditional methods for evaluating seeds are usually performed through destructive sampling followed by physical, physiological, biochemical and molecular determinations. Whilst proven to be effective, these approaches can be criticized as being destructive, time consuming, labor intensive and requiring experienced seed analysts. Thus, the objective of this study was to investigate the potential of computer vision and multispectral imaging systems supported with multivariate analysis for high-throughput classification of cowpea (Vigna unguiculata) seeds. An automated computer-vision germination system was utilized for uninterrupted monitoring of seeds during imbibition and germination to identify different categories of all individual seeds. By using spectral signatures of single cowpea seeds extracted from multispectral images, different multivariate analysis models based on linear discriminant analysis (LDA) were developed for classifying the seeds into different categories according to ageing, viability, seedling condition and speed of germination. Results The results revealed that the LDA models had good accuracy in distinguishing ‘Aged’ and ‘Non-aged’ seeds with an overall correct classification (OCC) of 97.51, 96.76 and 97%, ‘Germinated’ and ‘Non-germinated’ seeds with OCC of 81.80, 79.05 and 81.0%, ‘Early germinated’, ‘Medium germinated’ and ‘Dead’ seeds with OCC of 77.21, 74.93 and 68.00% and among seeds that give ‘Normal’ and ‘Abnormal’ seedlings with OCC of 68.08, 64.34 and 62.00% in training, cross-validation and independent validation data sets, respectively. Image processing routines were also developed to exploit the full power of the multispectral imaging system in visualizing the difference among seed categories by applying the discriminant model in a pixel-wise manner. Conclusion The results demonstrated the capability of the multispectral imaging system in the ultraviolet, visible and shortwave near infrared range to provide the required information necessary for the discrimination of individual cowpea seeds to different classes. Considering the short time of image acquisition and limited sample preparation, this stat-of-the art multispectral imaging method and chemometric analysis in classifying seeds could be a valuable tool for on-line classification protocols in cost-effective real-time sorting and grading processes as it provides not only morphological and physical features but also chemical information for the seeds being examined. Implementing image processing algorithms specific for seed quality assessment along with the declining cost and increasing power of computer hardware is very efficient to make the development of such computer-integrated systems more attractive in automatic inspection of seed quality. Cowpea seeds (dpeaa)DE-He213 Black-eyed seeds (dpeaa)DE-He213 Plant development (dpeaa)DE-He213 Germination (dpeaa)DE-He213 Phenotyping (dpeaa)DE-He213 Chemometric (dpeaa)DE-He213 Multispectral imaging (dpeaa)DE-He213 Mandour, Nasser aut Wagner, Marie-Hélène aut Demilly, Didier aut Verdier, Jerome aut Belin, Etienne aut Rousseau, David aut Enthalten in Plant methods London : BioMed Central, 2005 15(2019), 1 vom: 12. März (DE-627)500321191 (DE-600)2203723-8 1746-4811 nnns volume:15 year:2019 number:1 day:12 month:03 https://dx.doi.org/10.1186/s13007-019-0411-2 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2019 1 12 03 |
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10.1186/s13007-019-0411-2 doi (DE-627)SPR029411025 (SPR)s13007-019-0411-2-e DE-627 ger DE-627 rakwb eng ElMasry, Gamal verfasserin aut Utilization of computer vision and multispectral imaging techniques for classification of cowpea (Vigna unguiculata) seeds 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2019 Background The traditional methods for evaluating seeds are usually performed through destructive sampling followed by physical, physiological, biochemical and molecular determinations. Whilst proven to be effective, these approaches can be criticized as being destructive, time consuming, labor intensive and requiring experienced seed analysts. Thus, the objective of this study was to investigate the potential of computer vision and multispectral imaging systems supported with multivariate analysis for high-throughput classification of cowpea (Vigna unguiculata) seeds. An automated computer-vision germination system was utilized for uninterrupted monitoring of seeds during imbibition and germination to identify different categories of all individual seeds. By using spectral signatures of single cowpea seeds extracted from multispectral images, different multivariate analysis models based on linear discriminant analysis (LDA) were developed for classifying the seeds into different categories according to ageing, viability, seedling condition and speed of germination. Results The results revealed that the LDA models had good accuracy in distinguishing ‘Aged’ and ‘Non-aged’ seeds with an overall correct classification (OCC) of 97.51, 96.76 and 97%, ‘Germinated’ and ‘Non-germinated’ seeds with OCC of 81.80, 79.05 and 81.0%, ‘Early germinated’, ‘Medium germinated’ and ‘Dead’ seeds with OCC of 77.21, 74.93 and 68.00% and among seeds that give ‘Normal’ and ‘Abnormal’ seedlings with OCC of 68.08, 64.34 and 62.00% in training, cross-validation and independent validation data sets, respectively. Image processing routines were also developed to exploit the full power of the multispectral imaging system in visualizing the difference among seed categories by applying the discriminant model in a pixel-wise manner. Conclusion The results demonstrated the capability of the multispectral imaging system in the ultraviolet, visible and shortwave near infrared range to provide the required information necessary for the discrimination of individual cowpea seeds to different classes. Considering the short time of image acquisition and limited sample preparation, this stat-of-the art multispectral imaging method and chemometric analysis in classifying seeds could be a valuable tool for on-line classification protocols in cost-effective real-time sorting and grading processes as it provides not only morphological and physical features but also chemical information for the seeds being examined. Implementing image processing algorithms specific for seed quality assessment along with the declining cost and increasing power of computer hardware is very efficient to make the development of such computer-integrated systems more attractive in automatic inspection of seed quality. Cowpea seeds (dpeaa)DE-He213 Black-eyed seeds (dpeaa)DE-He213 Plant development (dpeaa)DE-He213 Germination (dpeaa)DE-He213 Phenotyping (dpeaa)DE-He213 Chemometric (dpeaa)DE-He213 Multispectral imaging (dpeaa)DE-He213 Mandour, Nasser aut Wagner, Marie-Hélène aut Demilly, Didier aut Verdier, Jerome aut Belin, Etienne aut Rousseau, David aut Enthalten in Plant methods London : BioMed Central, 2005 15(2019), 1 vom: 12. März (DE-627)500321191 (DE-600)2203723-8 1746-4811 nnns volume:15 year:2019 number:1 day:12 month:03 https://dx.doi.org/10.1186/s13007-019-0411-2 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2019 1 12 03 |
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utilization of computer vision and multispectral imaging techniques for classification of cowpea (vigna unguiculata) seeds |
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Utilization of computer vision and multispectral imaging techniques for classification of cowpea (Vigna unguiculata) seeds |
abstract |
Background The traditional methods for evaluating seeds are usually performed through destructive sampling followed by physical, physiological, biochemical and molecular determinations. Whilst proven to be effective, these approaches can be criticized as being destructive, time consuming, labor intensive and requiring experienced seed analysts. Thus, the objective of this study was to investigate the potential of computer vision and multispectral imaging systems supported with multivariate analysis for high-throughput classification of cowpea (Vigna unguiculata) seeds. An automated computer-vision germination system was utilized for uninterrupted monitoring of seeds during imbibition and germination to identify different categories of all individual seeds. By using spectral signatures of single cowpea seeds extracted from multispectral images, different multivariate analysis models based on linear discriminant analysis (LDA) were developed for classifying the seeds into different categories according to ageing, viability, seedling condition and speed of germination. Results The results revealed that the LDA models had good accuracy in distinguishing ‘Aged’ and ‘Non-aged’ seeds with an overall correct classification (OCC) of 97.51, 96.76 and 97%, ‘Germinated’ and ‘Non-germinated’ seeds with OCC of 81.80, 79.05 and 81.0%, ‘Early germinated’, ‘Medium germinated’ and ‘Dead’ seeds with OCC of 77.21, 74.93 and 68.00% and among seeds that give ‘Normal’ and ‘Abnormal’ seedlings with OCC of 68.08, 64.34 and 62.00% in training, cross-validation and independent validation data sets, respectively. Image processing routines were also developed to exploit the full power of the multispectral imaging system in visualizing the difference among seed categories by applying the discriminant model in a pixel-wise manner. Conclusion The results demonstrated the capability of the multispectral imaging system in the ultraviolet, visible and shortwave near infrared range to provide the required information necessary for the discrimination of individual cowpea seeds to different classes. Considering the short time of image acquisition and limited sample preparation, this stat-of-the art multispectral imaging method and chemometric analysis in classifying seeds could be a valuable tool for on-line classification protocols in cost-effective real-time sorting and grading processes as it provides not only morphological and physical features but also chemical information for the seeds being examined. Implementing image processing algorithms specific for seed quality assessment along with the declining cost and increasing power of computer hardware is very efficient to make the development of such computer-integrated systems more attractive in automatic inspection of seed quality. © The Author(s) 2019 |
abstractGer |
Background The traditional methods for evaluating seeds are usually performed through destructive sampling followed by physical, physiological, biochemical and molecular determinations. Whilst proven to be effective, these approaches can be criticized as being destructive, time consuming, labor intensive and requiring experienced seed analysts. Thus, the objective of this study was to investigate the potential of computer vision and multispectral imaging systems supported with multivariate analysis for high-throughput classification of cowpea (Vigna unguiculata) seeds. An automated computer-vision germination system was utilized for uninterrupted monitoring of seeds during imbibition and germination to identify different categories of all individual seeds. By using spectral signatures of single cowpea seeds extracted from multispectral images, different multivariate analysis models based on linear discriminant analysis (LDA) were developed for classifying the seeds into different categories according to ageing, viability, seedling condition and speed of germination. Results The results revealed that the LDA models had good accuracy in distinguishing ‘Aged’ and ‘Non-aged’ seeds with an overall correct classification (OCC) of 97.51, 96.76 and 97%, ‘Germinated’ and ‘Non-germinated’ seeds with OCC of 81.80, 79.05 and 81.0%, ‘Early germinated’, ‘Medium germinated’ and ‘Dead’ seeds with OCC of 77.21, 74.93 and 68.00% and among seeds that give ‘Normal’ and ‘Abnormal’ seedlings with OCC of 68.08, 64.34 and 62.00% in training, cross-validation and independent validation data sets, respectively. Image processing routines were also developed to exploit the full power of the multispectral imaging system in visualizing the difference among seed categories by applying the discriminant model in a pixel-wise manner. Conclusion The results demonstrated the capability of the multispectral imaging system in the ultraviolet, visible and shortwave near infrared range to provide the required information necessary for the discrimination of individual cowpea seeds to different classes. Considering the short time of image acquisition and limited sample preparation, this stat-of-the art multispectral imaging method and chemometric analysis in classifying seeds could be a valuable tool for on-line classification protocols in cost-effective real-time sorting and grading processes as it provides not only morphological and physical features but also chemical information for the seeds being examined. Implementing image processing algorithms specific for seed quality assessment along with the declining cost and increasing power of computer hardware is very efficient to make the development of such computer-integrated systems more attractive in automatic inspection of seed quality. © The Author(s) 2019 |
abstract_unstemmed |
Background The traditional methods for evaluating seeds are usually performed through destructive sampling followed by physical, physiological, biochemical and molecular determinations. Whilst proven to be effective, these approaches can be criticized as being destructive, time consuming, labor intensive and requiring experienced seed analysts. Thus, the objective of this study was to investigate the potential of computer vision and multispectral imaging systems supported with multivariate analysis for high-throughput classification of cowpea (Vigna unguiculata) seeds. An automated computer-vision germination system was utilized for uninterrupted monitoring of seeds during imbibition and germination to identify different categories of all individual seeds. By using spectral signatures of single cowpea seeds extracted from multispectral images, different multivariate analysis models based on linear discriminant analysis (LDA) were developed for classifying the seeds into different categories according to ageing, viability, seedling condition and speed of germination. Results The results revealed that the LDA models had good accuracy in distinguishing ‘Aged’ and ‘Non-aged’ seeds with an overall correct classification (OCC) of 97.51, 96.76 and 97%, ‘Germinated’ and ‘Non-germinated’ seeds with OCC of 81.80, 79.05 and 81.0%, ‘Early germinated’, ‘Medium germinated’ and ‘Dead’ seeds with OCC of 77.21, 74.93 and 68.00% and among seeds that give ‘Normal’ and ‘Abnormal’ seedlings with OCC of 68.08, 64.34 and 62.00% in training, cross-validation and independent validation data sets, respectively. Image processing routines were also developed to exploit the full power of the multispectral imaging system in visualizing the difference among seed categories by applying the discriminant model in a pixel-wise manner. Conclusion The results demonstrated the capability of the multispectral imaging system in the ultraviolet, visible and shortwave near infrared range to provide the required information necessary for the discrimination of individual cowpea seeds to different classes. Considering the short time of image acquisition and limited sample preparation, this stat-of-the art multispectral imaging method and chemometric analysis in classifying seeds could be a valuable tool for on-line classification protocols in cost-effective real-time sorting and grading processes as it provides not only morphological and physical features but also chemical information for the seeds being examined. Implementing image processing algorithms specific for seed quality assessment along with the declining cost and increasing power of computer hardware is very efficient to make the development of such computer-integrated systems more attractive in automatic inspection of seed quality. © The Author(s) 2019 |
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title_short |
Utilization of computer vision and multispectral imaging techniques for classification of cowpea (Vigna unguiculata) seeds |
url |
https://dx.doi.org/10.1186/s13007-019-0411-2 |
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Mandour, Nasser Wagner, Marie-Hélène Demilly, Didier Verdier, Jerome Belin, Etienne Rousseau, David |
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Mandour, Nasser Wagner, Marie-Hélène Demilly, Didier Verdier, Jerome Belin, Etienne Rousseau, David |
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up_date |
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