Characterization of clinical extensively drug-resistant Pseudomonas aeruginosa in the Hunan province of China

Background Pseudomonas aeruginosa strains that are classed as extensively drug resistant (XDR-PA) are resistant to all antibiotics except for one or two classes and are frequently the cause of hard-to-treat infections worldwide. Our study aimed to characterize clinical XDR-PA isolates recovered during 2011–2012 at nine hospitals in the Hunan province of China. Methods Thirty-seven non-repetitive XDR-PA strains from 37 patients were investigated for genes encoding antimicrobial resistance determinants, efflux pumps, outer membrane proteins, and movable genetic elements using polymerase chain reaction (PCR). The expression of genes encoding the efflux pump component MexA and the outer membrane protein OprD was measured using real-time PCR. In addition, clonal relatedness of these XDR-PA isolates was analyzed by pulsed-field gel electrophoresis (PFGE). Results Various genes encoding antimicrobial resistance determinants were found in all isolates. In particular, the blaTEM-1, blaCARB, armA, blaIMP-4, blaVIM-2, and rmtB, were found in 100, 37.8, 22, 22, 19 and 5 % of the isolates, respectively. Remarkably, two isolates coharbored blaIMP-4, blaVIM-2, and armA. In all 37 antibiotic-resistant strains, the relative expression of oprD was decreased while mexA was increased compared to the expression of these genes in antibiotic-susceptible P. aeruginosa strains. All of the XDR-PA isolates harbored class I integrons as well as multiple other mobile genetic elements, such as tnpU, tnp513, tnpA (Tn21), and merA. A high genotypic diversity among the strains was detected by PFGE. Conclusions Multiple antibiotic-resistance mechanisms contributed to the drug resistance of the XDR-PA isolates investigated in this study. Thus, the XDR-PA isolates in this area were not clonally related. Instead, multiple types of movable genetic elements were coharbored within each XDR-PA isolate, which may have aided the rapid development of these XDR-PA strains. This is the first report of XDR-PA strains that coharbor blaIMP-4, blaVIM-2, and armA. Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Li, Jun [verfasserIn] Zou, Mingxiang Dou, Qingya Hu, Yongmei Wang, Haichen Yan, Qun Liu, Wen’ en

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2016

Schlagwörter:	Extensively drug-resistant Resistance mechanism Movable genetic elements Molecular epidemiology PFGE

Anmerkung:	© The Author(s). 2016

Übergeordnetes Werk:	Enthalten in: Annals of clinical microbiology and antimicrobials - London : Biomed Central, 2002, 15(2016), 1 vom: 23. Mai
Übergeordnetes Werk:	volume:15 ; year:2016 ; number:1 ; day:23 ; month:05

Links:	Volltext

DOI / URN:	10.1186/s12941-016-0148-y

Katalog-ID:	SPR029437733

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520			\|a Background Pseudomonas aeruginosa strains that are classed as extensively drug resistant (XDR-PA) are resistant to all antibiotics except for one or two classes and are frequently the cause of hard-to-treat infections worldwide. Our study aimed to characterize clinical XDR-PA isolates recovered during 2011–2012 at nine hospitals in the Hunan province of China. Methods Thirty-seven non-repetitive XDR-PA strains from 37 patients were investigated for genes encoding antimicrobial resistance determinants, efflux pumps, outer membrane proteins, and movable genetic elements using polymerase chain reaction (PCR). The expression of genes encoding the efflux pump component MexA and the outer membrane protein OprD was measured using real-time PCR. In addition, clonal relatedness of these XDR-PA isolates was analyzed by pulsed-field gel electrophoresis (PFGE). Results Various genes encoding antimicrobial resistance determinants were found in all isolates. In particular, the blaTEM-1, blaCARB, armA, blaIMP-4, blaVIM-2, and rmtB, were found in 100, 37.8, 22, 22, 19 and 5 % of the isolates, respectively. Remarkably, two isolates coharbored blaIMP-4, blaVIM-2, and armA. In all 37 antibiotic-resistant strains, the relative expression of oprD was decreased while mexA was increased compared to the expression of these genes in antibiotic-susceptible P. aeruginosa strains. All of the XDR-PA isolates harbored class I integrons as well as multiple other mobile genetic elements, such as tnpU, tnp513, tnpA (Tn21), and merA. A high genotypic diversity among the strains was detected by PFGE. Conclusions Multiple antibiotic-resistance mechanisms contributed to the drug resistance of the XDR-PA isolates investigated in this study. Thus, the XDR-PA isolates in this area were not clonally related. Instead, multiple types of movable genetic elements were coharbored within each XDR-PA isolate, which may have aided the rapid development of these XDR-PA strains. This is the first report of XDR-PA strains that coharbor blaIMP-4, blaVIM-2, and armA.
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allfields	10.1186/s12941-016-0148-y doi (DE-627)SPR029437733 (SPR)s12941-016-0148-y-e DE-627 ger DE-627 rakwb eng Li, Jun verfasserin aut Characterization of clinical extensively drug-resistant Pseudomonas aeruginosa in the Hunan province of China 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2016 Background Pseudomonas aeruginosa strains that are classed as extensively drug resistant (XDR-PA) are resistant to all antibiotics except for one or two classes and are frequently the cause of hard-to-treat infections worldwide. Our study aimed to characterize clinical XDR-PA isolates recovered during 2011–2012 at nine hospitals in the Hunan province of China. Methods Thirty-seven non-repetitive XDR-PA strains from 37 patients were investigated for genes encoding antimicrobial resistance determinants, efflux pumps, outer membrane proteins, and movable genetic elements using polymerase chain reaction (PCR). The expression of genes encoding the efflux pump component MexA and the outer membrane protein OprD was measured using real-time PCR. In addition, clonal relatedness of these XDR-PA isolates was analyzed by pulsed-field gel electrophoresis (PFGE). Results Various genes encoding antimicrobial resistance determinants were found in all isolates. In particular, the blaTEM-1, blaCARB, armA, blaIMP-4, blaVIM-2, and rmtB, were found in 100, 37.8, 22, 22, 19 and 5 % of the isolates, respectively. Remarkably, two isolates coharbored blaIMP-4, blaVIM-2, and armA. In all 37 antibiotic-resistant strains, the relative expression of oprD was decreased while mexA was increased compared to the expression of these genes in antibiotic-susceptible P. aeruginosa strains. All of the XDR-PA isolates harbored class I integrons as well as multiple other mobile genetic elements, such as tnpU, tnp513, tnpA (Tn21), and merA. A high genotypic diversity among the strains was detected by PFGE. Conclusions Multiple antibiotic-resistance mechanisms contributed to the drug resistance of the XDR-PA isolates investigated in this study. Thus, the XDR-PA isolates in this area were not clonally related. Instead, multiple types of movable genetic elements were coharbored within each XDR-PA isolate, which may have aided the rapid development of these XDR-PA strains. This is the first report of XDR-PA strains that coharbor blaIMP-4, blaVIM-2, and armA. Extensively drug-resistant (dpeaa)DE-He213 Resistance mechanism (dpeaa)DE-He213 Movable genetic elements (dpeaa)DE-He213 Molecular epidemiology (dpeaa)DE-He213 PFGE (dpeaa)DE-He213 Zou, Mingxiang aut Dou, Qingya aut Hu, Yongmei aut Wang, Haichen aut Yan, Qun aut Liu, Wen’ en aut Enthalten in Annals of clinical microbiology and antimicrobials London : Biomed Central, 2002 15(2016), 1 vom: 23. Mai (DE-627)359783430 (DE-600)2097873-X 1476-0711 nnns volume:15 year:2016 number:1 day:23 month:05 https://dx.doi.org/10.1186/s12941-016-0148-y kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2016 1 23 05
spelling	10.1186/s12941-016-0148-y doi (DE-627)SPR029437733 (SPR)s12941-016-0148-y-e DE-627 ger DE-627 rakwb eng Li, Jun verfasserin aut Characterization of clinical extensively drug-resistant Pseudomonas aeruginosa in the Hunan province of China 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2016 Background Pseudomonas aeruginosa strains that are classed as extensively drug resistant (XDR-PA) are resistant to all antibiotics except for one or two classes and are frequently the cause of hard-to-treat infections worldwide. Our study aimed to characterize clinical XDR-PA isolates recovered during 2011–2012 at nine hospitals in the Hunan province of China. Methods Thirty-seven non-repetitive XDR-PA strains from 37 patients were investigated for genes encoding antimicrobial resistance determinants, efflux pumps, outer membrane proteins, and movable genetic elements using polymerase chain reaction (PCR). The expression of genes encoding the efflux pump component MexA and the outer membrane protein OprD was measured using real-time PCR. In addition, clonal relatedness of these XDR-PA isolates was analyzed by pulsed-field gel electrophoresis (PFGE). Results Various genes encoding antimicrobial resistance determinants were found in all isolates. In particular, the blaTEM-1, blaCARB, armA, blaIMP-4, blaVIM-2, and rmtB, were found in 100, 37.8, 22, 22, 19 and 5 % of the isolates, respectively. Remarkably, two isolates coharbored blaIMP-4, blaVIM-2, and armA. In all 37 antibiotic-resistant strains, the relative expression of oprD was decreased while mexA was increased compared to the expression of these genes in antibiotic-susceptible P. aeruginosa strains. All of the XDR-PA isolates harbored class I integrons as well as multiple other mobile genetic elements, such as tnpU, tnp513, tnpA (Tn21), and merA. A high genotypic diversity among the strains was detected by PFGE. Conclusions Multiple antibiotic-resistance mechanisms contributed to the drug resistance of the XDR-PA isolates investigated in this study. Thus, the XDR-PA isolates in this area were not clonally related. Instead, multiple types of movable genetic elements were coharbored within each XDR-PA isolate, which may have aided the rapid development of these XDR-PA strains. This is the first report of XDR-PA strains that coharbor blaIMP-4, blaVIM-2, and armA. Extensively drug-resistant (dpeaa)DE-He213 Resistance mechanism (dpeaa)DE-He213 Movable genetic elements (dpeaa)DE-He213 Molecular epidemiology (dpeaa)DE-He213 PFGE (dpeaa)DE-He213 Zou, Mingxiang aut Dou, Qingya aut Hu, Yongmei aut Wang, Haichen aut Yan, Qun aut Liu, Wen’ en aut Enthalten in Annals of clinical microbiology and antimicrobials London : Biomed Central, 2002 15(2016), 1 vom: 23. Mai (DE-627)359783430 (DE-600)2097873-X 1476-0711 nnns volume:15 year:2016 number:1 day:23 month:05 https://dx.doi.org/10.1186/s12941-016-0148-y kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2016 1 23 05
allfields_unstemmed	10.1186/s12941-016-0148-y doi (DE-627)SPR029437733 (SPR)s12941-016-0148-y-e DE-627 ger DE-627 rakwb eng Li, Jun verfasserin aut Characterization of clinical extensively drug-resistant Pseudomonas aeruginosa in the Hunan province of China 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2016 Background Pseudomonas aeruginosa strains that are classed as extensively drug resistant (XDR-PA) are resistant to all antibiotics except for one or two classes and are frequently the cause of hard-to-treat infections worldwide. Our study aimed to characterize clinical XDR-PA isolates recovered during 2011–2012 at nine hospitals in the Hunan province of China. Methods Thirty-seven non-repetitive XDR-PA strains from 37 patients were investigated for genes encoding antimicrobial resistance determinants, efflux pumps, outer membrane proteins, and movable genetic elements using polymerase chain reaction (PCR). The expression of genes encoding the efflux pump component MexA and the outer membrane protein OprD was measured using real-time PCR. In addition, clonal relatedness of these XDR-PA isolates was analyzed by pulsed-field gel electrophoresis (PFGE). Results Various genes encoding antimicrobial resistance determinants were found in all isolates. In particular, the blaTEM-1, blaCARB, armA, blaIMP-4, blaVIM-2, and rmtB, were found in 100, 37.8, 22, 22, 19 and 5 % of the isolates, respectively. Remarkably, two isolates coharbored blaIMP-4, blaVIM-2, and armA. In all 37 antibiotic-resistant strains, the relative expression of oprD was decreased while mexA was increased compared to the expression of these genes in antibiotic-susceptible P. aeruginosa strains. All of the XDR-PA isolates harbored class I integrons as well as multiple other mobile genetic elements, such as tnpU, tnp513, tnpA (Tn21), and merA. A high genotypic diversity among the strains was detected by PFGE. Conclusions Multiple antibiotic-resistance mechanisms contributed to the drug resistance of the XDR-PA isolates investigated in this study. Thus, the XDR-PA isolates in this area were not clonally related. Instead, multiple types of movable genetic elements were coharbored within each XDR-PA isolate, which may have aided the rapid development of these XDR-PA strains. This is the first report of XDR-PA strains that coharbor blaIMP-4, blaVIM-2, and armA. Extensively drug-resistant (dpeaa)DE-He213 Resistance mechanism (dpeaa)DE-He213 Movable genetic elements (dpeaa)DE-He213 Molecular epidemiology (dpeaa)DE-He213 PFGE (dpeaa)DE-He213 Zou, Mingxiang aut Dou, Qingya aut Hu, Yongmei aut Wang, Haichen aut Yan, Qun aut Liu, Wen’ en aut Enthalten in Annals of clinical microbiology and antimicrobials London : Biomed Central, 2002 15(2016), 1 vom: 23. Mai (DE-627)359783430 (DE-600)2097873-X 1476-0711 nnns volume:15 year:2016 number:1 day:23 month:05 https://dx.doi.org/10.1186/s12941-016-0148-y kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2016 1 23 05
allfieldsGer	10.1186/s12941-016-0148-y doi (DE-627)SPR029437733 (SPR)s12941-016-0148-y-e DE-627 ger DE-627 rakwb eng Li, Jun verfasserin aut Characterization of clinical extensively drug-resistant Pseudomonas aeruginosa in the Hunan province of China 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2016 Background Pseudomonas aeruginosa strains that are classed as extensively drug resistant (XDR-PA) are resistant to all antibiotics except for one or two classes and are frequently the cause of hard-to-treat infections worldwide. Our study aimed to characterize clinical XDR-PA isolates recovered during 2011–2012 at nine hospitals in the Hunan province of China. Methods Thirty-seven non-repetitive XDR-PA strains from 37 patients were investigated for genes encoding antimicrobial resistance determinants, efflux pumps, outer membrane proteins, and movable genetic elements using polymerase chain reaction (PCR). The expression of genes encoding the efflux pump component MexA and the outer membrane protein OprD was measured using real-time PCR. In addition, clonal relatedness of these XDR-PA isolates was analyzed by pulsed-field gel electrophoresis (PFGE). Results Various genes encoding antimicrobial resistance determinants were found in all isolates. In particular, the blaTEM-1, blaCARB, armA, blaIMP-4, blaVIM-2, and rmtB, were found in 100, 37.8, 22, 22, 19 and 5 % of the isolates, respectively. Remarkably, two isolates coharbored blaIMP-4, blaVIM-2, and armA. In all 37 antibiotic-resistant strains, the relative expression of oprD was decreased while mexA was increased compared to the expression of these genes in antibiotic-susceptible P. aeruginosa strains. All of the XDR-PA isolates harbored class I integrons as well as multiple other mobile genetic elements, such as tnpU, tnp513, tnpA (Tn21), and merA. A high genotypic diversity among the strains was detected by PFGE. Conclusions Multiple antibiotic-resistance mechanisms contributed to the drug resistance of the XDR-PA isolates investigated in this study. Thus, the XDR-PA isolates in this area were not clonally related. Instead, multiple types of movable genetic elements were coharbored within each XDR-PA isolate, which may have aided the rapid development of these XDR-PA strains. This is the first report of XDR-PA strains that coharbor blaIMP-4, blaVIM-2, and armA. Extensively drug-resistant (dpeaa)DE-He213 Resistance mechanism (dpeaa)DE-He213 Movable genetic elements (dpeaa)DE-He213 Molecular epidemiology (dpeaa)DE-He213 PFGE (dpeaa)DE-He213 Zou, Mingxiang aut Dou, Qingya aut Hu, Yongmei aut Wang, Haichen aut Yan, Qun aut Liu, Wen’ en aut Enthalten in Annals of clinical microbiology and antimicrobials London : Biomed Central, 2002 15(2016), 1 vom: 23. Mai (DE-627)359783430 (DE-600)2097873-X 1476-0711 nnns volume:15 year:2016 number:1 day:23 month:05 https://dx.doi.org/10.1186/s12941-016-0148-y kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2016 1 23 05
allfieldsSound	10.1186/s12941-016-0148-y doi (DE-627)SPR029437733 (SPR)s12941-016-0148-y-e DE-627 ger DE-627 rakwb eng Li, Jun verfasserin aut Characterization of clinical extensively drug-resistant Pseudomonas aeruginosa in the Hunan province of China 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2016 Background Pseudomonas aeruginosa strains that are classed as extensively drug resistant (XDR-PA) are resistant to all antibiotics except for one or two classes and are frequently the cause of hard-to-treat infections worldwide. Our study aimed to characterize clinical XDR-PA isolates recovered during 2011–2012 at nine hospitals in the Hunan province of China. Methods Thirty-seven non-repetitive XDR-PA strains from 37 patients were investigated for genes encoding antimicrobial resistance determinants, efflux pumps, outer membrane proteins, and movable genetic elements using polymerase chain reaction (PCR). The expression of genes encoding the efflux pump component MexA and the outer membrane protein OprD was measured using real-time PCR. In addition, clonal relatedness of these XDR-PA isolates was analyzed by pulsed-field gel electrophoresis (PFGE). Results Various genes encoding antimicrobial resistance determinants were found in all isolates. In particular, the blaTEM-1, blaCARB, armA, blaIMP-4, blaVIM-2, and rmtB, were found in 100, 37.8, 22, 22, 19 and 5 % of the isolates, respectively. Remarkably, two isolates coharbored blaIMP-4, blaVIM-2, and armA. In all 37 antibiotic-resistant strains, the relative expression of oprD was decreased while mexA was increased compared to the expression of these genes in antibiotic-susceptible P. aeruginosa strains. All of the XDR-PA isolates harbored class I integrons as well as multiple other mobile genetic elements, such as tnpU, tnp513, tnpA (Tn21), and merA. A high genotypic diversity among the strains was detected by PFGE. Conclusions Multiple antibiotic-resistance mechanisms contributed to the drug resistance of the XDR-PA isolates investigated in this study. Thus, the XDR-PA isolates in this area were not clonally related. Instead, multiple types of movable genetic elements were coharbored within each XDR-PA isolate, which may have aided the rapid development of these XDR-PA strains. This is the first report of XDR-PA strains that coharbor blaIMP-4, blaVIM-2, and armA. Extensively drug-resistant (dpeaa)DE-He213 Resistance mechanism (dpeaa)DE-He213 Movable genetic elements (dpeaa)DE-He213 Molecular epidemiology (dpeaa)DE-He213 PFGE (dpeaa)DE-He213 Zou, Mingxiang aut Dou, Qingya aut Hu, Yongmei aut Wang, Haichen aut Yan, Qun aut Liu, Wen’ en aut Enthalten in Annals of clinical microbiology and antimicrobials London : Biomed Central, 2002 15(2016), 1 vom: 23. Mai (DE-627)359783430 (DE-600)2097873-X 1476-0711 nnns volume:15 year:2016 number:1 day:23 month:05 https://dx.doi.org/10.1186/s12941-016-0148-y kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2016 1 23 05
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source	Enthalten in Annals of clinical microbiology and antimicrobials 15(2016), 1 vom: 23. Mai volume:15 year:2016 number:1 day:23 month:05
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spellingShingle	Li, Jun misc Extensively drug-resistant misc Resistance mechanism misc Movable genetic elements misc Molecular epidemiology misc PFGE Characterization of clinical extensively drug-resistant Pseudomonas aeruginosa in the Hunan province of China
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topic_title	Characterization of clinical extensively drug-resistant Pseudomonas aeruginosa in the Hunan province of China Extensively drug-resistant (dpeaa)DE-He213 Resistance mechanism (dpeaa)DE-He213 Movable genetic elements (dpeaa)DE-He213 Molecular epidemiology (dpeaa)DE-He213 PFGE (dpeaa)DE-He213
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title	Characterization of clinical extensively drug-resistant Pseudomonas aeruginosa in the Hunan province of China
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title_full	Characterization of clinical extensively drug-resistant Pseudomonas aeruginosa in the Hunan province of China
author_sort	Li, Jun
journal	Annals of clinical microbiology and antimicrobials
journalStr	Annals of clinical microbiology and antimicrobials
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author_browse	Li, Jun Zou, Mingxiang Dou, Qingya Hu, Yongmei Wang, Haichen Yan, Qun Liu, Wen’ en
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doi_str_mv	10.1186/s12941-016-0148-y
title_sort	characterization of clinical extensively drug-resistant pseudomonas aeruginosa in the hunan province of china
title_auth	Characterization of clinical extensively drug-resistant Pseudomonas aeruginosa in the Hunan province of China
abstract	Background Pseudomonas aeruginosa strains that are classed as extensively drug resistant (XDR-PA) are resistant to all antibiotics except for one or two classes and are frequently the cause of hard-to-treat infections worldwide. Our study aimed to characterize clinical XDR-PA isolates recovered during 2011–2012 at nine hospitals in the Hunan province of China. Methods Thirty-seven non-repetitive XDR-PA strains from 37 patients were investigated for genes encoding antimicrobial resistance determinants, efflux pumps, outer membrane proteins, and movable genetic elements using polymerase chain reaction (PCR). The expression of genes encoding the efflux pump component MexA and the outer membrane protein OprD was measured using real-time PCR. In addition, clonal relatedness of these XDR-PA isolates was analyzed by pulsed-field gel electrophoresis (PFGE). Results Various genes encoding antimicrobial resistance determinants were found in all isolates. In particular, the blaTEM-1, blaCARB, armA, blaIMP-4, blaVIM-2, and rmtB, were found in 100, 37.8, 22, 22, 19 and 5 % of the isolates, respectively. Remarkably, two isolates coharbored blaIMP-4, blaVIM-2, and armA. In all 37 antibiotic-resistant strains, the relative expression of oprD was decreased while mexA was increased compared to the expression of these genes in antibiotic-susceptible P. aeruginosa strains. All of the XDR-PA isolates harbored class I integrons as well as multiple other mobile genetic elements, such as tnpU, tnp513, tnpA (Tn21), and merA. A high genotypic diversity among the strains was detected by PFGE. Conclusions Multiple antibiotic-resistance mechanisms contributed to the drug resistance of the XDR-PA isolates investigated in this study. Thus, the XDR-PA isolates in this area were not clonally related. Instead, multiple types of movable genetic elements were coharbored within each XDR-PA isolate, which may have aided the rapid development of these XDR-PA strains. This is the first report of XDR-PA strains that coharbor blaIMP-4, blaVIM-2, and armA. © The Author(s). 2016
abstractGer	Background Pseudomonas aeruginosa strains that are classed as extensively drug resistant (XDR-PA) are resistant to all antibiotics except for one or two classes and are frequently the cause of hard-to-treat infections worldwide. Our study aimed to characterize clinical XDR-PA isolates recovered during 2011–2012 at nine hospitals in the Hunan province of China. Methods Thirty-seven non-repetitive XDR-PA strains from 37 patients were investigated for genes encoding antimicrobial resistance determinants, efflux pumps, outer membrane proteins, and movable genetic elements using polymerase chain reaction (PCR). The expression of genes encoding the efflux pump component MexA and the outer membrane protein OprD was measured using real-time PCR. In addition, clonal relatedness of these XDR-PA isolates was analyzed by pulsed-field gel electrophoresis (PFGE). Results Various genes encoding antimicrobial resistance determinants were found in all isolates. In particular, the blaTEM-1, blaCARB, armA, blaIMP-4, blaVIM-2, and rmtB, were found in 100, 37.8, 22, 22, 19 and 5 % of the isolates, respectively. Remarkably, two isolates coharbored blaIMP-4, blaVIM-2, and armA. In all 37 antibiotic-resistant strains, the relative expression of oprD was decreased while mexA was increased compared to the expression of these genes in antibiotic-susceptible P. aeruginosa strains. All of the XDR-PA isolates harbored class I integrons as well as multiple other mobile genetic elements, such as tnpU, tnp513, tnpA (Tn21), and merA. A high genotypic diversity among the strains was detected by PFGE. Conclusions Multiple antibiotic-resistance mechanisms contributed to the drug resistance of the XDR-PA isolates investigated in this study. Thus, the XDR-PA isolates in this area were not clonally related. Instead, multiple types of movable genetic elements were coharbored within each XDR-PA isolate, which may have aided the rapid development of these XDR-PA strains. This is the first report of XDR-PA strains that coharbor blaIMP-4, blaVIM-2, and armA. © The Author(s). 2016
abstract_unstemmed	Background Pseudomonas aeruginosa strains that are classed as extensively drug resistant (XDR-PA) are resistant to all antibiotics except for one or two classes and are frequently the cause of hard-to-treat infections worldwide. Our study aimed to characterize clinical XDR-PA isolates recovered during 2011–2012 at nine hospitals in the Hunan province of China. Methods Thirty-seven non-repetitive XDR-PA strains from 37 patients were investigated for genes encoding antimicrobial resistance determinants, efflux pumps, outer membrane proteins, and movable genetic elements using polymerase chain reaction (PCR). The expression of genes encoding the efflux pump component MexA and the outer membrane protein OprD was measured using real-time PCR. In addition, clonal relatedness of these XDR-PA isolates was analyzed by pulsed-field gel electrophoresis (PFGE). Results Various genes encoding antimicrobial resistance determinants were found in all isolates. In particular, the blaTEM-1, blaCARB, armA, blaIMP-4, blaVIM-2, and rmtB, were found in 100, 37.8, 22, 22, 19 and 5 % of the isolates, respectively. Remarkably, two isolates coharbored blaIMP-4, blaVIM-2, and armA. In all 37 antibiotic-resistant strains, the relative expression of oprD was decreased while mexA was increased compared to the expression of these genes in antibiotic-susceptible P. aeruginosa strains. All of the XDR-PA isolates harbored class I integrons as well as multiple other mobile genetic elements, such as tnpU, tnp513, tnpA (Tn21), and merA. A high genotypic diversity among the strains was detected by PFGE. Conclusions Multiple antibiotic-resistance mechanisms contributed to the drug resistance of the XDR-PA isolates investigated in this study. Thus, the XDR-PA isolates in this area were not clonally related. Instead, multiple types of movable genetic elements were coharbored within each XDR-PA isolate, which may have aided the rapid development of these XDR-PA strains. This is the first report of XDR-PA strains that coharbor blaIMP-4, blaVIM-2, and armA. © The Author(s). 2016
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title_short	Characterization of clinical extensively drug-resistant Pseudomonas aeruginosa in the Hunan province of China
url	https://dx.doi.org/10.1186/s12941-016-0148-y
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author2	Zou, Mingxiang Dou, Qingya Hu, Yongmei Wang, Haichen Yan, Qun Liu, Wen’ en
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doi_str	10.1186/s12941-016-0148-y
up_date	2024-07-04T00:58:02.076Z
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