Sustained delivery of siRNA/PEI complex from in situ forming hydrogels potently inhibits the proliferation of gastric cancer

Background Gastric cancer remains a major cause of mortality and morbidity worldwide. In recent years, gene-based therapeutic strategies were confirmed promising in cancer inhibition and attracted great attention. RNA interference (RNAi) is a powerful tool for gene therapy and has been widely employ...
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Autor*in:	Peng, Hao [verfasserIn] Yang, Huawei Song, Liwei Zhou, Zheng Sun, Jinwen Du, Yunfeng Lu, Keyu Li, Tao Yin, Aiguo Xu, Jianhua Wei, Shidong

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2016

Schlagwörter:	siRNA Collagen hydrogel Id1 Gastric cancer

Anmerkung:	© Peng et al. 2016

Übergeordnetes Werk:	Enthalten in: Journal of experimental & clinical cancer research - Berlin : Springer, 2008, 35(2016), 1 vom: 31. März
Übergeordnetes Werk:	volume:35 ; year:2016 ; number:1 ; day:31 ; month:03

Links:	Volltext

DOI / URN:	10.1186/s13046-016-0334-y

Katalog-ID:	SPR029637481

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520			\|a Background Gastric cancer remains a major cause of mortality and morbidity worldwide. In recent years, gene-based therapeutic strategies were confirmed promising in cancer inhibition and attracted great attention. RNA interference (RNAi) is a powerful tool for gene therapy and has been widely employed to aid in treatment for various diseases, especially cancers. However, effective delivery of small interfering RNA (siRNA) to target cells in vivo remains a challenge for that it is prone to degradation and only lasts a few days in rapidly dividing cells. Methods Due to its biocompatibility and well-established safety profile, collagen represents a favourable matrix for in-site drug delivery. In the study, collagen hydrogel was used as carriers to test the feasibility of localized and sustained delivery of Id1-targeted siRNA for in vivo gastric cancer inhibition. To enhance the siRNA delivery, cationic polyethylenimine (PEI) was further emplored for scallold modification. The efficacy of siRNA delivery and cancer inhibition were evaluated with multimodality of mehods in vitro and in vivo. Results Our results showed that addition of polyethylenimine (PEI) to collagen can facilitate entry of Id1-siRNA into target cells, prolong the silencing effect, and further inhibit tumor growth both in vitro and in vivo. Conclusion This collagen-based delivery system may facilitate the pathogenesis elucidation and design of effective therapies against gastric cancer.
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700	1		\|a Wei, Shidong \|4 aut
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allfields	10.1186/s13046-016-0334-y doi (DE-627)SPR029637481 (SPR)s13046-016-0334-y-e DE-627 ger DE-627 rakwb eng Peng, Hao verfasserin aut Sustained delivery of siRNA/PEI complex from in situ forming hydrogels potently inhibits the proliferation of gastric cancer 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Peng et al. 2016 Background Gastric cancer remains a major cause of mortality and morbidity worldwide. In recent years, gene-based therapeutic strategies were confirmed promising in cancer inhibition and attracted great attention. RNA interference (RNAi) is a powerful tool for gene therapy and has been widely employed to aid in treatment for various diseases, especially cancers. However, effective delivery of small interfering RNA (siRNA) to target cells in vivo remains a challenge for that it is prone to degradation and only lasts a few days in rapidly dividing cells. Methods Due to its biocompatibility and well-established safety profile, collagen represents a favourable matrix for in-site drug delivery. In the study, collagen hydrogel was used as carriers to test the feasibility of localized and sustained delivery of Id1-targeted siRNA for in vivo gastric cancer inhibition. To enhance the siRNA delivery, cationic polyethylenimine (PEI) was further emplored for scallold modification. The efficacy of siRNA delivery and cancer inhibition were evaluated with multimodality of mehods in vitro and in vivo. Results Our results showed that addition of polyethylenimine (PEI) to collagen can facilitate entry of Id1-siRNA into target cells, prolong the silencing effect, and further inhibit tumor growth both in vitro and in vivo. Conclusion This collagen-based delivery system may facilitate the pathogenesis elucidation and design of effective therapies against gastric cancer. siRNA (dpeaa)DE-He213 Collagen hydrogel (dpeaa)DE-He213 Id1 (dpeaa)DE-He213 Gastric cancer (dpeaa)DE-He213 Yang, Huawei aut Song, Liwei aut Zhou, Zheng aut Sun, Jinwen aut Du, Yunfeng aut Lu, Keyu aut Li, Tao aut Yin, Aiguo aut Xu, Jianhua aut Wei, Shidong aut Enthalten in Journal of experimental & clinical cancer research Berlin : Springer, 2008 35(2016), 1 vom: 31. März (DE-627)568921380 (DE-600)2430698-8 1756-9966 nnns volume:35 year:2016 number:1 day:31 month:03 https://dx.doi.org/10.1186/s13046-016-0334-y kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 35 2016 1 31 03
spelling	10.1186/s13046-016-0334-y doi (DE-627)SPR029637481 (SPR)s13046-016-0334-y-e DE-627 ger DE-627 rakwb eng Peng, Hao verfasserin aut Sustained delivery of siRNA/PEI complex from in situ forming hydrogels potently inhibits the proliferation of gastric cancer 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Peng et al. 2016 Background Gastric cancer remains a major cause of mortality and morbidity worldwide. In recent years, gene-based therapeutic strategies were confirmed promising in cancer inhibition and attracted great attention. RNA interference (RNAi) is a powerful tool for gene therapy and has been widely employed to aid in treatment for various diseases, especially cancers. However, effective delivery of small interfering RNA (siRNA) to target cells in vivo remains a challenge for that it is prone to degradation and only lasts a few days in rapidly dividing cells. Methods Due to its biocompatibility and well-established safety profile, collagen represents a favourable matrix for in-site drug delivery. In the study, collagen hydrogel was used as carriers to test the feasibility of localized and sustained delivery of Id1-targeted siRNA for in vivo gastric cancer inhibition. To enhance the siRNA delivery, cationic polyethylenimine (PEI) was further emplored for scallold modification. The efficacy of siRNA delivery and cancer inhibition were evaluated with multimodality of mehods in vitro and in vivo. Results Our results showed that addition of polyethylenimine (PEI) to collagen can facilitate entry of Id1-siRNA into target cells, prolong the silencing effect, and further inhibit tumor growth both in vitro and in vivo. Conclusion This collagen-based delivery system may facilitate the pathogenesis elucidation and design of effective therapies against gastric cancer. siRNA (dpeaa)DE-He213 Collagen hydrogel (dpeaa)DE-He213 Id1 (dpeaa)DE-He213 Gastric cancer (dpeaa)DE-He213 Yang, Huawei aut Song, Liwei aut Zhou, Zheng aut Sun, Jinwen aut Du, Yunfeng aut Lu, Keyu aut Li, Tao aut Yin, Aiguo aut Xu, Jianhua aut Wei, Shidong aut Enthalten in Journal of experimental & clinical cancer research Berlin : Springer, 2008 35(2016), 1 vom: 31. März (DE-627)568921380 (DE-600)2430698-8 1756-9966 nnns volume:35 year:2016 number:1 day:31 month:03 https://dx.doi.org/10.1186/s13046-016-0334-y kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 35 2016 1 31 03
allfields_unstemmed	10.1186/s13046-016-0334-y doi (DE-627)SPR029637481 (SPR)s13046-016-0334-y-e DE-627 ger DE-627 rakwb eng Peng, Hao verfasserin aut Sustained delivery of siRNA/PEI complex from in situ forming hydrogels potently inhibits the proliferation of gastric cancer 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Peng et al. 2016 Background Gastric cancer remains a major cause of mortality and morbidity worldwide. In recent years, gene-based therapeutic strategies were confirmed promising in cancer inhibition and attracted great attention. RNA interference (RNAi) is a powerful tool for gene therapy and has been widely employed to aid in treatment for various diseases, especially cancers. However, effective delivery of small interfering RNA (siRNA) to target cells in vivo remains a challenge for that it is prone to degradation and only lasts a few days in rapidly dividing cells. Methods Due to its biocompatibility and well-established safety profile, collagen represents a favourable matrix for in-site drug delivery. In the study, collagen hydrogel was used as carriers to test the feasibility of localized and sustained delivery of Id1-targeted siRNA for in vivo gastric cancer inhibition. To enhance the siRNA delivery, cationic polyethylenimine (PEI) was further emplored for scallold modification. The efficacy of siRNA delivery and cancer inhibition were evaluated with multimodality of mehods in vitro and in vivo. Results Our results showed that addition of polyethylenimine (PEI) to collagen can facilitate entry of Id1-siRNA into target cells, prolong the silencing effect, and further inhibit tumor growth both in vitro and in vivo. Conclusion This collagen-based delivery system may facilitate the pathogenesis elucidation and design of effective therapies against gastric cancer. siRNA (dpeaa)DE-He213 Collagen hydrogel (dpeaa)DE-He213 Id1 (dpeaa)DE-He213 Gastric cancer (dpeaa)DE-He213 Yang, Huawei aut Song, Liwei aut Zhou, Zheng aut Sun, Jinwen aut Du, Yunfeng aut Lu, Keyu aut Li, Tao aut Yin, Aiguo aut Xu, Jianhua aut Wei, Shidong aut Enthalten in Journal of experimental & clinical cancer research Berlin : Springer, 2008 35(2016), 1 vom: 31. März (DE-627)568921380 (DE-600)2430698-8 1756-9966 nnns volume:35 year:2016 number:1 day:31 month:03 https://dx.doi.org/10.1186/s13046-016-0334-y kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 35 2016 1 31 03
allfieldsGer	10.1186/s13046-016-0334-y doi (DE-627)SPR029637481 (SPR)s13046-016-0334-y-e DE-627 ger DE-627 rakwb eng Peng, Hao verfasserin aut Sustained delivery of siRNA/PEI complex from in situ forming hydrogels potently inhibits the proliferation of gastric cancer 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Peng et al. 2016 Background Gastric cancer remains a major cause of mortality and morbidity worldwide. In recent years, gene-based therapeutic strategies were confirmed promising in cancer inhibition and attracted great attention. RNA interference (RNAi) is a powerful tool for gene therapy and has been widely employed to aid in treatment for various diseases, especially cancers. However, effective delivery of small interfering RNA (siRNA) to target cells in vivo remains a challenge for that it is prone to degradation and only lasts a few days in rapidly dividing cells. Methods Due to its biocompatibility and well-established safety profile, collagen represents a favourable matrix for in-site drug delivery. In the study, collagen hydrogel was used as carriers to test the feasibility of localized and sustained delivery of Id1-targeted siRNA for in vivo gastric cancer inhibition. To enhance the siRNA delivery, cationic polyethylenimine (PEI) was further emplored for scallold modification. The efficacy of siRNA delivery and cancer inhibition were evaluated with multimodality of mehods in vitro and in vivo. Results Our results showed that addition of polyethylenimine (PEI) to collagen can facilitate entry of Id1-siRNA into target cells, prolong the silencing effect, and further inhibit tumor growth both in vitro and in vivo. Conclusion This collagen-based delivery system may facilitate the pathogenesis elucidation and design of effective therapies against gastric cancer. siRNA (dpeaa)DE-He213 Collagen hydrogel (dpeaa)DE-He213 Id1 (dpeaa)DE-He213 Gastric cancer (dpeaa)DE-He213 Yang, Huawei aut Song, Liwei aut Zhou, Zheng aut Sun, Jinwen aut Du, Yunfeng aut Lu, Keyu aut Li, Tao aut Yin, Aiguo aut Xu, Jianhua aut Wei, Shidong aut Enthalten in Journal of experimental & clinical cancer research Berlin : Springer, 2008 35(2016), 1 vom: 31. März (DE-627)568921380 (DE-600)2430698-8 1756-9966 nnns volume:35 year:2016 number:1 day:31 month:03 https://dx.doi.org/10.1186/s13046-016-0334-y kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 35 2016 1 31 03
allfieldsSound	10.1186/s13046-016-0334-y doi (DE-627)SPR029637481 (SPR)s13046-016-0334-y-e DE-627 ger DE-627 rakwb eng Peng, Hao verfasserin aut Sustained delivery of siRNA/PEI complex from in situ forming hydrogels potently inhibits the proliferation of gastric cancer 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Peng et al. 2016 Background Gastric cancer remains a major cause of mortality and morbidity worldwide. In recent years, gene-based therapeutic strategies were confirmed promising in cancer inhibition and attracted great attention. RNA interference (RNAi) is a powerful tool for gene therapy and has been widely employed to aid in treatment for various diseases, especially cancers. However, effective delivery of small interfering RNA (siRNA) to target cells in vivo remains a challenge for that it is prone to degradation and only lasts a few days in rapidly dividing cells. Methods Due to its biocompatibility and well-established safety profile, collagen represents a favourable matrix for in-site drug delivery. In the study, collagen hydrogel was used as carriers to test the feasibility of localized and sustained delivery of Id1-targeted siRNA for in vivo gastric cancer inhibition. To enhance the siRNA delivery, cationic polyethylenimine (PEI) was further emplored for scallold modification. The efficacy of siRNA delivery and cancer inhibition were evaluated with multimodality of mehods in vitro and in vivo. Results Our results showed that addition of polyethylenimine (PEI) to collagen can facilitate entry of Id1-siRNA into target cells, prolong the silencing effect, and further inhibit tumor growth both in vitro and in vivo. Conclusion This collagen-based delivery system may facilitate the pathogenesis elucidation and design of effective therapies against gastric cancer. siRNA (dpeaa)DE-He213 Collagen hydrogel (dpeaa)DE-He213 Id1 (dpeaa)DE-He213 Gastric cancer (dpeaa)DE-He213 Yang, Huawei aut Song, Liwei aut Zhou, Zheng aut Sun, Jinwen aut Du, Yunfeng aut Lu, Keyu aut Li, Tao aut Yin, Aiguo aut Xu, Jianhua aut Wei, Shidong aut Enthalten in Journal of experimental & clinical cancer research Berlin : Springer, 2008 35(2016), 1 vom: 31. März (DE-627)568921380 (DE-600)2430698-8 1756-9966 nnns volume:35 year:2016 number:1 day:31 month:03 https://dx.doi.org/10.1186/s13046-016-0334-y kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 35 2016 1 31 03
language	English
source	Enthalten in Journal of experimental & clinical cancer research 35(2016), 1 vom: 31. März volume:35 year:2016 number:1 day:31 month:03
sourceStr	Enthalten in Journal of experimental & clinical cancer research 35(2016), 1 vom: 31. März volume:35 year:2016 number:1 day:31 month:03
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authorswithroles_txt_mv	Peng, Hao @@aut@@ Yang, Huawei @@aut@@ Song, Liwei @@aut@@ Zhou, Zheng @@aut@@ Sun, Jinwen @@aut@@ Du, Yunfeng @@aut@@ Lu, Keyu @@aut@@ Li, Tao @@aut@@ Yin, Aiguo @@aut@@ Xu, Jianhua @@aut@@ Wei, Shidong @@aut@@
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The efficacy of siRNA delivery and cancer inhibition were evaluated with multimodality of mehods in vitro and in vivo. Results Our results showed that addition of polyethylenimine (PEI) to collagen can facilitate entry of Id1-siRNA into target cells, prolong the silencing effect, and further inhibit tumor growth both in vitro and in vivo. 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author	Peng, Hao
spellingShingle	Peng, Hao misc siRNA misc Collagen hydrogel misc Id1 misc Gastric cancer Sustained delivery of siRNA/PEI complex from in situ forming hydrogels potently inhibits the proliferation of gastric cancer
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topic_title	Sustained delivery of siRNA/PEI complex from in situ forming hydrogels potently inhibits the proliferation of gastric cancer siRNA (dpeaa)DE-He213 Collagen hydrogel (dpeaa)DE-He213 Id1 (dpeaa)DE-He213 Gastric cancer (dpeaa)DE-He213
topic	misc siRNA misc Collagen hydrogel misc Id1 misc Gastric cancer
topic_unstemmed	misc siRNA misc Collagen hydrogel misc Id1 misc Gastric cancer
topic_browse	misc siRNA misc Collagen hydrogel misc Id1 misc Gastric cancer
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title	Sustained delivery of siRNA/PEI complex from in situ forming hydrogels potently inhibits the proliferation of gastric cancer
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title_full	Sustained delivery of siRNA/PEI complex from in situ forming hydrogels potently inhibits the proliferation of gastric cancer
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author_browse	Peng, Hao Yang, Huawei Song, Liwei Zhou, Zheng Sun, Jinwen Du, Yunfeng Lu, Keyu Li, Tao Yin, Aiguo Xu, Jianhua Wei, Shidong
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title_sort	sustained delivery of sirna/pei complex from in situ forming hydrogels potently inhibits the proliferation of gastric cancer
title_auth	Sustained delivery of siRNA/PEI complex from in situ forming hydrogels potently inhibits the proliferation of gastric cancer
abstract	Background Gastric cancer remains a major cause of mortality and morbidity worldwide. In recent years, gene-based therapeutic strategies were confirmed promising in cancer inhibition and attracted great attention. RNA interference (RNAi) is a powerful tool for gene therapy and has been widely employed to aid in treatment for various diseases, especially cancers. However, effective delivery of small interfering RNA (siRNA) to target cells in vivo remains a challenge for that it is prone to degradation and only lasts a few days in rapidly dividing cells. Methods Due to its biocompatibility and well-established safety profile, collagen represents a favourable matrix for in-site drug delivery. In the study, collagen hydrogel was used as carriers to test the feasibility of localized and sustained delivery of Id1-targeted siRNA for in vivo gastric cancer inhibition. To enhance the siRNA delivery, cationic polyethylenimine (PEI) was further emplored for scallold modification. The efficacy of siRNA delivery and cancer inhibition were evaluated with multimodality of mehods in vitro and in vivo. Results Our results showed that addition of polyethylenimine (PEI) to collagen can facilitate entry of Id1-siRNA into target cells, prolong the silencing effect, and further inhibit tumor growth both in vitro and in vivo. Conclusion This collagen-based delivery system may facilitate the pathogenesis elucidation and design of effective therapies against gastric cancer. © Peng et al. 2016
abstractGer	Background Gastric cancer remains a major cause of mortality and morbidity worldwide. In recent years, gene-based therapeutic strategies were confirmed promising in cancer inhibition and attracted great attention. RNA interference (RNAi) is a powerful tool for gene therapy and has been widely employed to aid in treatment for various diseases, especially cancers. However, effective delivery of small interfering RNA (siRNA) to target cells in vivo remains a challenge for that it is prone to degradation and only lasts a few days in rapidly dividing cells. Methods Due to its biocompatibility and well-established safety profile, collagen represents a favourable matrix for in-site drug delivery. In the study, collagen hydrogel was used as carriers to test the feasibility of localized and sustained delivery of Id1-targeted siRNA for in vivo gastric cancer inhibition. To enhance the siRNA delivery, cationic polyethylenimine (PEI) was further emplored for scallold modification. The efficacy of siRNA delivery and cancer inhibition were evaluated with multimodality of mehods in vitro and in vivo. Results Our results showed that addition of polyethylenimine (PEI) to collagen can facilitate entry of Id1-siRNA into target cells, prolong the silencing effect, and further inhibit tumor growth both in vitro and in vivo. Conclusion This collagen-based delivery system may facilitate the pathogenesis elucidation and design of effective therapies against gastric cancer. © Peng et al. 2016
abstract_unstemmed	Background Gastric cancer remains a major cause of mortality and morbidity worldwide. In recent years, gene-based therapeutic strategies were confirmed promising in cancer inhibition and attracted great attention. RNA interference (RNAi) is a powerful tool for gene therapy and has been widely employed to aid in treatment for various diseases, especially cancers. However, effective delivery of small interfering RNA (siRNA) to target cells in vivo remains a challenge for that it is prone to degradation and only lasts a few days in rapidly dividing cells. Methods Due to its biocompatibility and well-established safety profile, collagen represents a favourable matrix for in-site drug delivery. In the study, collagen hydrogel was used as carriers to test the feasibility of localized and sustained delivery of Id1-targeted siRNA for in vivo gastric cancer inhibition. To enhance the siRNA delivery, cationic polyethylenimine (PEI) was further emplored for scallold modification. The efficacy of siRNA delivery and cancer inhibition were evaluated with multimodality of mehods in vitro and in vivo. Results Our results showed that addition of polyethylenimine (PEI) to collagen can facilitate entry of Id1-siRNA into target cells, prolong the silencing effect, and further inhibit tumor growth both in vitro and in vivo. Conclusion This collagen-based delivery system may facilitate the pathogenesis elucidation and design of effective therapies against gastric cancer. © Peng et al. 2016
collection_details	GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700
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title_short	Sustained delivery of siRNA/PEI complex from in situ forming hydrogels potently inhibits the proliferation of gastric cancer
url	https://dx.doi.org/10.1186/s13046-016-0334-y
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author2	Yang, Huawei Song, Liwei Zhou, Zheng Sun, Jinwen Du, Yunfeng Lu, Keyu Li, Tao Yin, Aiguo Xu, Jianhua Wei, Shidong
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up_date	2024-07-04T01:48:31.370Z
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The efficacy of siRNA delivery and cancer inhibition were evaluated with multimodality of mehods in vitro and in vivo. Results Our results showed that addition of polyethylenimine (PEI) to collagen can facilitate entry of Id1-siRNA into target cells, prolong the silencing effect, and further inhibit tumor growth both in vitro and in vivo. 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Sustained delivery of siRNA/PEI complex from in situ forming hydrogels potently inhibits the proliferation of gastric cancer

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