Signatures of human regulatory T cells: an encounter with old friends and new players
Background Naturally occurring $ CD4^{+} %$ CD25^{+} $ regulatory T cells ($ T_{Reg} $) are involved in the control of autoimmune diseases, transplantation tolerance, and anti-tumor immunity. Thus far, genomic studies on $ T_{Reg} $ cells were restricted to murine systems, and requirements for their...
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| Autor*in: |
Pfoertner, Susanne [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2006 |
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| Anmerkung: |
© Pfoertner et al.; licensee BioMed Central Ltd. 2006. This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License ( |
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| Übergeordnetes Werk: |
Enthalten in: Genome biology - London : BioMed Central, 2000, 7(2006), 7 vom: 12. Juli |
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| Übergeordnetes Werk: |
volume:7 ; year:2006 ; number:7 ; day:12 ; month:07 |
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| DOI / URN: |
10.1186/gb-2006-7-7-r54 |
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SPR029996376 |
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| 520 | |a Background Naturally occurring $ CD4^{+} %$ CD25^{+} $ regulatory T cells ($ T_{Reg} $) are involved in the control of autoimmune diseases, transplantation tolerance, and anti-tumor immunity. Thus far, genomic studies on $ T_{Reg} $ cells were restricted to murine systems, and requirements for their development, maintenance, and mode of action in humans are poorly defined. Results To improve characterization of human $ T_{Reg} $ cells, we compiled a unique microarray consisting of 350 $ T_{Reg} $ cell associated genes (Human $ T_{Reg} $ Chip) based on whole genome transcription data from human and mouse $ T_{Reg} $ cells. $ T_{Reg} $ cell specific gene signatures were created from 11 individual healthy donors. Statistical analysis identified 62 genes differentially expressed in $ T_{Reg} $ cells, emphasizing some cross-species differences between mice and humans. Among them, several 'old friends' (including FOXP3, CTLA4, and CCR7) that are known to be involved in $ T_{Reg} $ cell function were recovered. Strikingly, the vast majority of genes identified had not previously been associated with human $ T_{Reg} $ cells (including LGALS3, TIAF1, and TRAF1). Most of these 'new players' however, have been described in the pathogenesis of autoimmunity. Real-time RT-PCR of selected genes validated our microarray results. Pathway analysis was applied to extract signaling modules underlying human $ T_{Reg} $ cell function. Conclusion The comprehensive set of genes reported here provides a defined starting point to unravel the unique characteristics of human $ T_{Reg} $ cells. The Human $ T_{Reg} $ Chip constructed and validated here is available to the scientific community and is a useful tool with which to study the molecular mechanisms that orchestrate $ T_{Reg} $ cells under physiologic and diseased conditions. | ||
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| 700 | 1 | |a Jeron, Andreas |4 aut | |
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| 700 | 1 | |a Westendorf, Astrid M |4 aut | |
| 700 | 1 | |a Toepfer, Tanja |4 aut | |
| 700 | 1 | |a Schrader, Andres J |4 aut | |
| 700 | 1 | |a Franzke, Anke |4 aut | |
| 700 | 1 | |a Buer, Jan |4 aut | |
| 700 | 1 | |a Geffers, Robert |4 aut | |
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10.1186/gb-2006-7-7-r54 doi (DE-627)SPR029996376 (SPR)gb-2006-7-7-r54-e DE-627 ger DE-627 rakwb eng Pfoertner, Susanne verfasserin aut Signatures of human regulatory T cells: an encounter with old friends and new players 2006 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Pfoertner et al.; licensee BioMed Central Ltd. 2006. This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License ( Background Naturally occurring $ CD4^{+} %$ CD25^{+} $ regulatory T cells ($ T_{Reg} $) are involved in the control of autoimmune diseases, transplantation tolerance, and anti-tumor immunity. Thus far, genomic studies on $ T_{Reg} $ cells were restricted to murine systems, and requirements for their development, maintenance, and mode of action in humans are poorly defined. Results To improve characterization of human $ T_{Reg} $ cells, we compiled a unique microarray consisting of 350 $ T_{Reg} $ cell associated genes (Human $ T_{Reg} $ Chip) based on whole genome transcription data from human and mouse $ T_{Reg} $ cells. $ T_{Reg} $ cell specific gene signatures were created from 11 individual healthy donors. Statistical analysis identified 62 genes differentially expressed in $ T_{Reg} $ cells, emphasizing some cross-species differences between mice and humans. Among them, several 'old friends' (including FOXP3, CTLA4, and CCR7) that are known to be involved in $ T_{Reg} $ cell function were recovered. Strikingly, the vast majority of genes identified had not previously been associated with human $ T_{Reg} $ cells (including LGALS3, TIAF1, and TRAF1). Most of these 'new players' however, have been described in the pathogenesis of autoimmunity. Real-time RT-PCR of selected genes validated our microarray results. Pathway analysis was applied to extract signaling modules underlying human $ T_{Reg} $ cell function. Conclusion The comprehensive set of genes reported here provides a defined starting point to unravel the unique characteristics of human $ T_{Reg} $ cells. The Human $ T_{Reg} $ Chip constructed and validated here is available to the scientific community and is a useful tool with which to study the molecular mechanisms that orchestrate $ T_{Reg} $ cells under physiologic and diseased conditions. TReg Cell (dpeaa)DE-He213 Additional Data File (dpeaa)DE-He213 Affymetrix GeneChip (dpeaa)DE-He213 Tumor Necrosis Factor Receptor Superfamily (dpeaa)DE-He213 Signal Intensity Difference (dpeaa)DE-He213 Jeron, Andreas aut Probst-Kepper, Michael aut Guzman, Carlos A aut Hansen, Wiebke aut Westendorf, Astrid M aut Toepfer, Tanja aut Schrader, Andres J aut Franzke, Anke aut Buer, Jan aut Geffers, Robert aut Enthalten in Genome biology London : BioMed Central, 2000 7(2006), 7 vom: 12. Juli (DE-627)326173617 (DE-600)2040529-7 1474-760X nnns volume:7 year:2006 number:7 day:12 month:07 https://dx.doi.org/10.1186/gb-2006-7-7-r54 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2006 7 12 07 |
| spelling |
10.1186/gb-2006-7-7-r54 doi (DE-627)SPR029996376 (SPR)gb-2006-7-7-r54-e DE-627 ger DE-627 rakwb eng Pfoertner, Susanne verfasserin aut Signatures of human regulatory T cells: an encounter with old friends and new players 2006 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Pfoertner et al.; licensee BioMed Central Ltd. 2006. This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License ( Background Naturally occurring $ CD4^{+} %$ CD25^{+} $ regulatory T cells ($ T_{Reg} $) are involved in the control of autoimmune diseases, transplantation tolerance, and anti-tumor immunity. Thus far, genomic studies on $ T_{Reg} $ cells were restricted to murine systems, and requirements for their development, maintenance, and mode of action in humans are poorly defined. Results To improve characterization of human $ T_{Reg} $ cells, we compiled a unique microarray consisting of 350 $ T_{Reg} $ cell associated genes (Human $ T_{Reg} $ Chip) based on whole genome transcription data from human and mouse $ T_{Reg} $ cells. $ T_{Reg} $ cell specific gene signatures were created from 11 individual healthy donors. Statistical analysis identified 62 genes differentially expressed in $ T_{Reg} $ cells, emphasizing some cross-species differences between mice and humans. Among them, several 'old friends' (including FOXP3, CTLA4, and CCR7) that are known to be involved in $ T_{Reg} $ cell function were recovered. Strikingly, the vast majority of genes identified had not previously been associated with human $ T_{Reg} $ cells (including LGALS3, TIAF1, and TRAF1). Most of these 'new players' however, have been described in the pathogenesis of autoimmunity. Real-time RT-PCR of selected genes validated our microarray results. Pathway analysis was applied to extract signaling modules underlying human $ T_{Reg} $ cell function. Conclusion The comprehensive set of genes reported here provides a defined starting point to unravel the unique characteristics of human $ T_{Reg} $ cells. The Human $ T_{Reg} $ Chip constructed and validated here is available to the scientific community and is a useful tool with which to study the molecular mechanisms that orchestrate $ T_{Reg} $ cells under physiologic and diseased conditions. TReg Cell (dpeaa)DE-He213 Additional Data File (dpeaa)DE-He213 Affymetrix GeneChip (dpeaa)DE-He213 Tumor Necrosis Factor Receptor Superfamily (dpeaa)DE-He213 Signal Intensity Difference (dpeaa)DE-He213 Jeron, Andreas aut Probst-Kepper, Michael aut Guzman, Carlos A aut Hansen, Wiebke aut Westendorf, Astrid M aut Toepfer, Tanja aut Schrader, Andres J aut Franzke, Anke aut Buer, Jan aut Geffers, Robert aut Enthalten in Genome biology London : BioMed Central, 2000 7(2006), 7 vom: 12. Juli (DE-627)326173617 (DE-600)2040529-7 1474-760X nnns volume:7 year:2006 number:7 day:12 month:07 https://dx.doi.org/10.1186/gb-2006-7-7-r54 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2006 7 12 07 |
| allfields_unstemmed |
10.1186/gb-2006-7-7-r54 doi (DE-627)SPR029996376 (SPR)gb-2006-7-7-r54-e DE-627 ger DE-627 rakwb eng Pfoertner, Susanne verfasserin aut Signatures of human regulatory T cells: an encounter with old friends and new players 2006 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Pfoertner et al.; licensee BioMed Central Ltd. 2006. This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License ( Background Naturally occurring $ CD4^{+} %$ CD25^{+} $ regulatory T cells ($ T_{Reg} $) are involved in the control of autoimmune diseases, transplantation tolerance, and anti-tumor immunity. Thus far, genomic studies on $ T_{Reg} $ cells were restricted to murine systems, and requirements for their development, maintenance, and mode of action in humans are poorly defined. Results To improve characterization of human $ T_{Reg} $ cells, we compiled a unique microarray consisting of 350 $ T_{Reg} $ cell associated genes (Human $ T_{Reg} $ Chip) based on whole genome transcription data from human and mouse $ T_{Reg} $ cells. $ T_{Reg} $ cell specific gene signatures were created from 11 individual healthy donors. Statistical analysis identified 62 genes differentially expressed in $ T_{Reg} $ cells, emphasizing some cross-species differences between mice and humans. Among them, several 'old friends' (including FOXP3, CTLA4, and CCR7) that are known to be involved in $ T_{Reg} $ cell function were recovered. Strikingly, the vast majority of genes identified had not previously been associated with human $ T_{Reg} $ cells (including LGALS3, TIAF1, and TRAF1). Most of these 'new players' however, have been described in the pathogenesis of autoimmunity. Real-time RT-PCR of selected genes validated our microarray results. Pathway analysis was applied to extract signaling modules underlying human $ T_{Reg} $ cell function. Conclusion The comprehensive set of genes reported here provides a defined starting point to unravel the unique characteristics of human $ T_{Reg} $ cells. The Human $ T_{Reg} $ Chip constructed and validated here is available to the scientific community and is a useful tool with which to study the molecular mechanisms that orchestrate $ T_{Reg} $ cells under physiologic and diseased conditions. TReg Cell (dpeaa)DE-He213 Additional Data File (dpeaa)DE-He213 Affymetrix GeneChip (dpeaa)DE-He213 Tumor Necrosis Factor Receptor Superfamily (dpeaa)DE-He213 Signal Intensity Difference (dpeaa)DE-He213 Jeron, Andreas aut Probst-Kepper, Michael aut Guzman, Carlos A aut Hansen, Wiebke aut Westendorf, Astrid M aut Toepfer, Tanja aut Schrader, Andres J aut Franzke, Anke aut Buer, Jan aut Geffers, Robert aut Enthalten in Genome biology London : BioMed Central, 2000 7(2006), 7 vom: 12. Juli (DE-627)326173617 (DE-600)2040529-7 1474-760X nnns volume:7 year:2006 number:7 day:12 month:07 https://dx.doi.org/10.1186/gb-2006-7-7-r54 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2006 7 12 07 |
| allfieldsGer |
10.1186/gb-2006-7-7-r54 doi (DE-627)SPR029996376 (SPR)gb-2006-7-7-r54-e DE-627 ger DE-627 rakwb eng Pfoertner, Susanne verfasserin aut Signatures of human regulatory T cells: an encounter with old friends and new players 2006 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Pfoertner et al.; licensee BioMed Central Ltd. 2006. This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License ( Background Naturally occurring $ CD4^{+} %$ CD25^{+} $ regulatory T cells ($ T_{Reg} $) are involved in the control of autoimmune diseases, transplantation tolerance, and anti-tumor immunity. Thus far, genomic studies on $ T_{Reg} $ cells were restricted to murine systems, and requirements for their development, maintenance, and mode of action in humans are poorly defined. Results To improve characterization of human $ T_{Reg} $ cells, we compiled a unique microarray consisting of 350 $ T_{Reg} $ cell associated genes (Human $ T_{Reg} $ Chip) based on whole genome transcription data from human and mouse $ T_{Reg} $ cells. $ T_{Reg} $ cell specific gene signatures were created from 11 individual healthy donors. Statistical analysis identified 62 genes differentially expressed in $ T_{Reg} $ cells, emphasizing some cross-species differences between mice and humans. Among them, several 'old friends' (including FOXP3, CTLA4, and CCR7) that are known to be involved in $ T_{Reg} $ cell function were recovered. Strikingly, the vast majority of genes identified had not previously been associated with human $ T_{Reg} $ cells (including LGALS3, TIAF1, and TRAF1). Most of these 'new players' however, have been described in the pathogenesis of autoimmunity. Real-time RT-PCR of selected genes validated our microarray results. Pathway analysis was applied to extract signaling modules underlying human $ T_{Reg} $ cell function. Conclusion The comprehensive set of genes reported here provides a defined starting point to unravel the unique characteristics of human $ T_{Reg} $ cells. The Human $ T_{Reg} $ Chip constructed and validated here is available to the scientific community and is a useful tool with which to study the molecular mechanisms that orchestrate $ T_{Reg} $ cells under physiologic and diseased conditions. TReg Cell (dpeaa)DE-He213 Additional Data File (dpeaa)DE-He213 Affymetrix GeneChip (dpeaa)DE-He213 Tumor Necrosis Factor Receptor Superfamily (dpeaa)DE-He213 Signal Intensity Difference (dpeaa)DE-He213 Jeron, Andreas aut Probst-Kepper, Michael aut Guzman, Carlos A aut Hansen, Wiebke aut Westendorf, Astrid M aut Toepfer, Tanja aut Schrader, Andres J aut Franzke, Anke aut Buer, Jan aut Geffers, Robert aut Enthalten in Genome biology London : BioMed Central, 2000 7(2006), 7 vom: 12. Juli (DE-627)326173617 (DE-600)2040529-7 1474-760X nnns volume:7 year:2006 number:7 day:12 month:07 https://dx.doi.org/10.1186/gb-2006-7-7-r54 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2006 7 12 07 |
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10.1186/gb-2006-7-7-r54 doi (DE-627)SPR029996376 (SPR)gb-2006-7-7-r54-e DE-627 ger DE-627 rakwb eng Pfoertner, Susanne verfasserin aut Signatures of human regulatory T cells: an encounter with old friends and new players 2006 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Pfoertner et al.; licensee BioMed Central Ltd. 2006. This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License ( Background Naturally occurring $ CD4^{+} %$ CD25^{+} $ regulatory T cells ($ T_{Reg} $) are involved in the control of autoimmune diseases, transplantation tolerance, and anti-tumor immunity. Thus far, genomic studies on $ T_{Reg} $ cells were restricted to murine systems, and requirements for their development, maintenance, and mode of action in humans are poorly defined. Results To improve characterization of human $ T_{Reg} $ cells, we compiled a unique microarray consisting of 350 $ T_{Reg} $ cell associated genes (Human $ T_{Reg} $ Chip) based on whole genome transcription data from human and mouse $ T_{Reg} $ cells. $ T_{Reg} $ cell specific gene signatures were created from 11 individual healthy donors. Statistical analysis identified 62 genes differentially expressed in $ T_{Reg} $ cells, emphasizing some cross-species differences between mice and humans. Among them, several 'old friends' (including FOXP3, CTLA4, and CCR7) that are known to be involved in $ T_{Reg} $ cell function were recovered. Strikingly, the vast majority of genes identified had not previously been associated with human $ T_{Reg} $ cells (including LGALS3, TIAF1, and TRAF1). Most of these 'new players' however, have been described in the pathogenesis of autoimmunity. Real-time RT-PCR of selected genes validated our microarray results. Pathway analysis was applied to extract signaling modules underlying human $ T_{Reg} $ cell function. Conclusion The comprehensive set of genes reported here provides a defined starting point to unravel the unique characteristics of human $ T_{Reg} $ cells. The Human $ T_{Reg} $ Chip constructed and validated here is available to the scientific community and is a useful tool with which to study the molecular mechanisms that orchestrate $ T_{Reg} $ cells under physiologic and diseased conditions. TReg Cell (dpeaa)DE-He213 Additional Data File (dpeaa)DE-He213 Affymetrix GeneChip (dpeaa)DE-He213 Tumor Necrosis Factor Receptor Superfamily (dpeaa)DE-He213 Signal Intensity Difference (dpeaa)DE-He213 Jeron, Andreas aut Probst-Kepper, Michael aut Guzman, Carlos A aut Hansen, Wiebke aut Westendorf, Astrid M aut Toepfer, Tanja aut Schrader, Andres J aut Franzke, Anke aut Buer, Jan aut Geffers, Robert aut Enthalten in Genome biology London : BioMed Central, 2000 7(2006), 7 vom: 12. Juli (DE-627)326173617 (DE-600)2040529-7 1474-760X nnns volume:7 year:2006 number:7 day:12 month:07 https://dx.doi.org/10.1186/gb-2006-7-7-r54 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2006 7 12 07 |
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Signatures of human regulatory T cells: an encounter with old friends and new players |
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Background Naturally occurring $ CD4^{+} %$ CD25^{+} $ regulatory T cells ($ T_{Reg} $) are involved in the control of autoimmune diseases, transplantation tolerance, and anti-tumor immunity. Thus far, genomic studies on $ T_{Reg} $ cells were restricted to murine systems, and requirements for their development, maintenance, and mode of action in humans are poorly defined. Results To improve characterization of human $ T_{Reg} $ cells, we compiled a unique microarray consisting of 350 $ T_{Reg} $ cell associated genes (Human $ T_{Reg} $ Chip) based on whole genome transcription data from human and mouse $ T_{Reg} $ cells. $ T_{Reg} $ cell specific gene signatures were created from 11 individual healthy donors. Statistical analysis identified 62 genes differentially expressed in $ T_{Reg} $ cells, emphasizing some cross-species differences between mice and humans. Among them, several 'old friends' (including FOXP3, CTLA4, and CCR7) that are known to be involved in $ T_{Reg} $ cell function were recovered. Strikingly, the vast majority of genes identified had not previously been associated with human $ T_{Reg} $ cells (including LGALS3, TIAF1, and TRAF1). Most of these 'new players' however, have been described in the pathogenesis of autoimmunity. Real-time RT-PCR of selected genes validated our microarray results. Pathway analysis was applied to extract signaling modules underlying human $ T_{Reg} $ cell function. Conclusion The comprehensive set of genes reported here provides a defined starting point to unravel the unique characteristics of human $ T_{Reg} $ cells. The Human $ T_{Reg} $ Chip constructed and validated here is available to the scientific community and is a useful tool with which to study the molecular mechanisms that orchestrate $ T_{Reg} $ cells under physiologic and diseased conditions. © Pfoertner et al.; licensee BioMed Central Ltd. 2006. This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License ( |
| abstractGer |
Background Naturally occurring $ CD4^{+} %$ CD25^{+} $ regulatory T cells ($ T_{Reg} $) are involved in the control of autoimmune diseases, transplantation tolerance, and anti-tumor immunity. Thus far, genomic studies on $ T_{Reg} $ cells were restricted to murine systems, and requirements for their development, maintenance, and mode of action in humans are poorly defined. Results To improve characterization of human $ T_{Reg} $ cells, we compiled a unique microarray consisting of 350 $ T_{Reg} $ cell associated genes (Human $ T_{Reg} $ Chip) based on whole genome transcription data from human and mouse $ T_{Reg} $ cells. $ T_{Reg} $ cell specific gene signatures were created from 11 individual healthy donors. Statistical analysis identified 62 genes differentially expressed in $ T_{Reg} $ cells, emphasizing some cross-species differences between mice and humans. Among them, several 'old friends' (including FOXP3, CTLA4, and CCR7) that are known to be involved in $ T_{Reg} $ cell function were recovered. Strikingly, the vast majority of genes identified had not previously been associated with human $ T_{Reg} $ cells (including LGALS3, TIAF1, and TRAF1). Most of these 'new players' however, have been described in the pathogenesis of autoimmunity. Real-time RT-PCR of selected genes validated our microarray results. Pathway analysis was applied to extract signaling modules underlying human $ T_{Reg} $ cell function. Conclusion The comprehensive set of genes reported here provides a defined starting point to unravel the unique characteristics of human $ T_{Reg} $ cells. The Human $ T_{Reg} $ Chip constructed and validated here is available to the scientific community and is a useful tool with which to study the molecular mechanisms that orchestrate $ T_{Reg} $ cells under physiologic and diseased conditions. © Pfoertner et al.; licensee BioMed Central Ltd. 2006. This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License ( |
| abstract_unstemmed |
Background Naturally occurring $ CD4^{+} %$ CD25^{+} $ regulatory T cells ($ T_{Reg} $) are involved in the control of autoimmune diseases, transplantation tolerance, and anti-tumor immunity. Thus far, genomic studies on $ T_{Reg} $ cells were restricted to murine systems, and requirements for their development, maintenance, and mode of action in humans are poorly defined. Results To improve characterization of human $ T_{Reg} $ cells, we compiled a unique microarray consisting of 350 $ T_{Reg} $ cell associated genes (Human $ T_{Reg} $ Chip) based on whole genome transcription data from human and mouse $ T_{Reg} $ cells. $ T_{Reg} $ cell specific gene signatures were created from 11 individual healthy donors. Statistical analysis identified 62 genes differentially expressed in $ T_{Reg} $ cells, emphasizing some cross-species differences between mice and humans. Among them, several 'old friends' (including FOXP3, CTLA4, and CCR7) that are known to be involved in $ T_{Reg} $ cell function were recovered. Strikingly, the vast majority of genes identified had not previously been associated with human $ T_{Reg} $ cells (including LGALS3, TIAF1, and TRAF1). Most of these 'new players' however, have been described in the pathogenesis of autoimmunity. Real-time RT-PCR of selected genes validated our microarray results. Pathway analysis was applied to extract signaling modules underlying human $ T_{Reg} $ cell function. Conclusion The comprehensive set of genes reported here provides a defined starting point to unravel the unique characteristics of human $ T_{Reg} $ cells. The Human $ T_{Reg} $ Chip constructed and validated here is available to the scientific community and is a useful tool with which to study the molecular mechanisms that orchestrate $ T_{Reg} $ cells under physiologic and diseased conditions. © Pfoertner et al.; licensee BioMed Central Ltd. 2006. This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License ( |
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Signatures of human regulatory T cells: an encounter with old friends and new players |
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Jeron, Andreas Probst-Kepper, Michael Guzman, Carlos A Hansen, Wiebke Westendorf, Astrid M Toepfer, Tanja Schrader, Andres J Franzke, Anke Buer, Jan Geffers, Robert |
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Jeron, Andreas Probst-Kepper, Michael Guzman, Carlos A Hansen, Wiebke Westendorf, Astrid M Toepfer, Tanja Schrader, Andres J Franzke, Anke Buer, Jan Geffers, Robert |
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