Rapid gene isolation in barley and wheat by mutant chromosome sequencing

Abstract Identification of causal mutations in barley and wheat is hampered by their large genomes and suppressed recombination. To overcome these obstacles, we have developed MutChromSeq, a complexity reduction approach based on flow sorting and sequencing of mutant chromosomes, to identify induced...
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Autor*in:	Sánchez-Martín, Javier [verfasserIn] Steuernagel, Burkhard Ghosh, Sreya Herren, Gerhard Hurni, Severine Adamski, Nikolai Vrána, Jan Kubaláková, Marie Krattinger, Simon G. Wicker, Thomas Doležel, Jaroslav Keller, Beat Wulff, Brande B. H.

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2016

Schlagwörter:	MutChromSeq Gene cloning Mutational genomics Chromosome flow sorting Triticeae Wheat Barley

Anmerkung:	© The Author(s). 2016

Übergeordnetes Werk:	Enthalten in: Genome biology - London : BioMed Central, 2000, 17(2016), 1 vom: 31. Okt.
Übergeordnetes Werk:	volume:17 ; year:2016 ; number:1 ; day:31 ; month:10

Links:	Volltext

DOI / URN:	10.1186/s13059-016-1082-1

Katalog-ID:	SPR030026075

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520			\|a Abstract Identification of causal mutations in barley and wheat is hampered by their large genomes and suppressed recombination. To overcome these obstacles, we have developed MutChromSeq, a complexity reduction approach based on flow sorting and sequencing of mutant chromosomes, to identify induced mutations by comparison to parental chromosomes. We apply MutChromSeq to six mutants each of the barley Eceriferum-q gene and the wheat Pm2 genes. This approach unambiguously identified single candidate genes that were verified by Sanger sequencing of additional mutants. MutChromSeq enables reference-free forward genetics in barley and wheat, thus opening up their pan-genomes to functional genomics.
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allfields	10.1186/s13059-016-1082-1 doi (DE-627)SPR030026075 (SPR)s13059-016-1082-1-e DE-627 ger DE-627 rakwb eng Sánchez-Martín, Javier verfasserin aut Rapid gene isolation in barley and wheat by mutant chromosome sequencing 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2016 Abstract Identification of causal mutations in barley and wheat is hampered by their large genomes and suppressed recombination. To overcome these obstacles, we have developed MutChromSeq, a complexity reduction approach based on flow sorting and sequencing of mutant chromosomes, to identify induced mutations by comparison to parental chromosomes. We apply MutChromSeq to six mutants each of the barley Eceriferum-q gene and the wheat Pm2 genes. This approach unambiguously identified single candidate genes that were verified by Sanger sequencing of additional mutants. MutChromSeq enables reference-free forward genetics in barley and wheat, thus opening up their pan-genomes to functional genomics. MutChromSeq (dpeaa)DE-He213 Gene cloning (dpeaa)DE-He213 Mutational genomics (dpeaa)DE-He213 Chromosome flow sorting (dpeaa)DE-He213 Triticeae (dpeaa)DE-He213 Wheat (dpeaa)DE-He213 Barley (dpeaa)DE-He213 Steuernagel, Burkhard aut Ghosh, Sreya aut Herren, Gerhard aut Hurni, Severine aut Adamski, Nikolai aut Vrána, Jan aut Kubaláková, Marie aut Krattinger, Simon G. aut Wicker, Thomas aut Doležel, Jaroslav aut Keller, Beat aut Wulff, Brande B. H. aut Enthalten in Genome biology London : BioMed Central, 2000 17(2016), 1 vom: 31. Okt. (DE-627)326173617 (DE-600)2040529-7 1474-760X nnns volume:17 year:2016 number:1 day:31 month:10 https://dx.doi.org/10.1186/s13059-016-1082-1 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 17 2016 1 31 10
spelling	10.1186/s13059-016-1082-1 doi (DE-627)SPR030026075 (SPR)s13059-016-1082-1-e DE-627 ger DE-627 rakwb eng Sánchez-Martín, Javier verfasserin aut Rapid gene isolation in barley and wheat by mutant chromosome sequencing 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2016 Abstract Identification of causal mutations in barley and wheat is hampered by their large genomes and suppressed recombination. To overcome these obstacles, we have developed MutChromSeq, a complexity reduction approach based on flow sorting and sequencing of mutant chromosomes, to identify induced mutations by comparison to parental chromosomes. We apply MutChromSeq to six mutants each of the barley Eceriferum-q gene and the wheat Pm2 genes. This approach unambiguously identified single candidate genes that were verified by Sanger sequencing of additional mutants. MutChromSeq enables reference-free forward genetics in barley and wheat, thus opening up their pan-genomes to functional genomics. MutChromSeq (dpeaa)DE-He213 Gene cloning (dpeaa)DE-He213 Mutational genomics (dpeaa)DE-He213 Chromosome flow sorting (dpeaa)DE-He213 Triticeae (dpeaa)DE-He213 Wheat (dpeaa)DE-He213 Barley (dpeaa)DE-He213 Steuernagel, Burkhard aut Ghosh, Sreya aut Herren, Gerhard aut Hurni, Severine aut Adamski, Nikolai aut Vrána, Jan aut Kubaláková, Marie aut Krattinger, Simon G. aut Wicker, Thomas aut Doležel, Jaroslav aut Keller, Beat aut Wulff, Brande B. H. aut Enthalten in Genome biology London : BioMed Central, 2000 17(2016), 1 vom: 31. Okt. (DE-627)326173617 (DE-600)2040529-7 1474-760X nnns volume:17 year:2016 number:1 day:31 month:10 https://dx.doi.org/10.1186/s13059-016-1082-1 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 17 2016 1 31 10
allfields_unstemmed	10.1186/s13059-016-1082-1 doi (DE-627)SPR030026075 (SPR)s13059-016-1082-1-e DE-627 ger DE-627 rakwb eng Sánchez-Martín, Javier verfasserin aut Rapid gene isolation in barley and wheat by mutant chromosome sequencing 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2016 Abstract Identification of causal mutations in barley and wheat is hampered by their large genomes and suppressed recombination. To overcome these obstacles, we have developed MutChromSeq, a complexity reduction approach based on flow sorting and sequencing of mutant chromosomes, to identify induced mutations by comparison to parental chromosomes. We apply MutChromSeq to six mutants each of the barley Eceriferum-q gene and the wheat Pm2 genes. This approach unambiguously identified single candidate genes that were verified by Sanger sequencing of additional mutants. MutChromSeq enables reference-free forward genetics in barley and wheat, thus opening up their pan-genomes to functional genomics. MutChromSeq (dpeaa)DE-He213 Gene cloning (dpeaa)DE-He213 Mutational genomics (dpeaa)DE-He213 Chromosome flow sorting (dpeaa)DE-He213 Triticeae (dpeaa)DE-He213 Wheat (dpeaa)DE-He213 Barley (dpeaa)DE-He213 Steuernagel, Burkhard aut Ghosh, Sreya aut Herren, Gerhard aut Hurni, Severine aut Adamski, Nikolai aut Vrána, Jan aut Kubaláková, Marie aut Krattinger, Simon G. aut Wicker, Thomas aut Doležel, Jaroslav aut Keller, Beat aut Wulff, Brande B. H. aut Enthalten in Genome biology London : BioMed Central, 2000 17(2016), 1 vom: 31. Okt. (DE-627)326173617 (DE-600)2040529-7 1474-760X nnns volume:17 year:2016 number:1 day:31 month:10 https://dx.doi.org/10.1186/s13059-016-1082-1 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 17 2016 1 31 10
allfieldsGer	10.1186/s13059-016-1082-1 doi (DE-627)SPR030026075 (SPR)s13059-016-1082-1-e DE-627 ger DE-627 rakwb eng Sánchez-Martín, Javier verfasserin aut Rapid gene isolation in barley and wheat by mutant chromosome sequencing 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2016 Abstract Identification of causal mutations in barley and wheat is hampered by their large genomes and suppressed recombination. To overcome these obstacles, we have developed MutChromSeq, a complexity reduction approach based on flow sorting and sequencing of mutant chromosomes, to identify induced mutations by comparison to parental chromosomes. We apply MutChromSeq to six mutants each of the barley Eceriferum-q gene and the wheat Pm2 genes. This approach unambiguously identified single candidate genes that were verified by Sanger sequencing of additional mutants. MutChromSeq enables reference-free forward genetics in barley and wheat, thus opening up their pan-genomes to functional genomics. MutChromSeq (dpeaa)DE-He213 Gene cloning (dpeaa)DE-He213 Mutational genomics (dpeaa)DE-He213 Chromosome flow sorting (dpeaa)DE-He213 Triticeae (dpeaa)DE-He213 Wheat (dpeaa)DE-He213 Barley (dpeaa)DE-He213 Steuernagel, Burkhard aut Ghosh, Sreya aut Herren, Gerhard aut Hurni, Severine aut Adamski, Nikolai aut Vrána, Jan aut Kubaláková, Marie aut Krattinger, Simon G. aut Wicker, Thomas aut Doležel, Jaroslav aut Keller, Beat aut Wulff, Brande B. H. aut Enthalten in Genome biology London : BioMed Central, 2000 17(2016), 1 vom: 31. Okt. (DE-627)326173617 (DE-600)2040529-7 1474-760X nnns volume:17 year:2016 number:1 day:31 month:10 https://dx.doi.org/10.1186/s13059-016-1082-1 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 17 2016 1 31 10
allfieldsSound	10.1186/s13059-016-1082-1 doi (DE-627)SPR030026075 (SPR)s13059-016-1082-1-e DE-627 ger DE-627 rakwb eng Sánchez-Martín, Javier verfasserin aut Rapid gene isolation in barley and wheat by mutant chromosome sequencing 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2016 Abstract Identification of causal mutations in barley and wheat is hampered by their large genomes and suppressed recombination. To overcome these obstacles, we have developed MutChromSeq, a complexity reduction approach based on flow sorting and sequencing of mutant chromosomes, to identify induced mutations by comparison to parental chromosomes. We apply MutChromSeq to six mutants each of the barley Eceriferum-q gene and the wheat Pm2 genes. This approach unambiguously identified single candidate genes that were verified by Sanger sequencing of additional mutants. MutChromSeq enables reference-free forward genetics in barley and wheat, thus opening up their pan-genomes to functional genomics. MutChromSeq (dpeaa)DE-He213 Gene cloning (dpeaa)DE-He213 Mutational genomics (dpeaa)DE-He213 Chromosome flow sorting (dpeaa)DE-He213 Triticeae (dpeaa)DE-He213 Wheat (dpeaa)DE-He213 Barley (dpeaa)DE-He213 Steuernagel, Burkhard aut Ghosh, Sreya aut Herren, Gerhard aut Hurni, Severine aut Adamski, Nikolai aut Vrána, Jan aut Kubaláková, Marie aut Krattinger, Simon G. aut Wicker, Thomas aut Doležel, Jaroslav aut Keller, Beat aut Wulff, Brande B. H. aut Enthalten in Genome biology London : BioMed Central, 2000 17(2016), 1 vom: 31. Okt. (DE-627)326173617 (DE-600)2040529-7 1474-760X nnns volume:17 year:2016 number:1 day:31 month:10 https://dx.doi.org/10.1186/s13059-016-1082-1 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 17 2016 1 31 10
language	English
source	Enthalten in Genome biology 17(2016), 1 vom: 31. Okt. volume:17 year:2016 number:1 day:31 month:10
sourceStr	Enthalten in Genome biology 17(2016), 1 vom: 31. Okt. volume:17 year:2016 number:1 day:31 month:10
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	MutChromSeq Gene cloning Mutational genomics Chromosome flow sorting Triticeae Wheat Barley
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container_title	Genome biology
authorswithroles_txt_mv	Sánchez-Martín, Javier @@aut@@ Steuernagel, Burkhard @@aut@@ Ghosh, Sreya @@aut@@ Herren, Gerhard @@aut@@ Hurni, Severine @@aut@@ Adamski, Nikolai @@aut@@ Vrána, Jan @@aut@@ Kubaláková, Marie @@aut@@ Krattinger, Simon G. @@aut@@ Wicker, Thomas @@aut@@ Doležel, Jaroslav @@aut@@ Keller, Beat @@aut@@ Wulff, Brande B. H. @@aut@@
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author	Sánchez-Martín, Javier
spellingShingle	Sánchez-Martín, Javier misc MutChromSeq misc Gene cloning misc Mutational genomics misc Chromosome flow sorting misc Triticeae misc Wheat misc Barley Rapid gene isolation in barley and wheat by mutant chromosome sequencing
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topic_title	Rapid gene isolation in barley and wheat by mutant chromosome sequencing MutChromSeq (dpeaa)DE-He213 Gene cloning (dpeaa)DE-He213 Mutational genomics (dpeaa)DE-He213 Chromosome flow sorting (dpeaa)DE-He213 Triticeae (dpeaa)DE-He213 Wheat (dpeaa)DE-He213 Barley (dpeaa)DE-He213
topic	misc MutChromSeq misc Gene cloning misc Mutational genomics misc Chromosome flow sorting misc Triticeae misc Wheat misc Barley
topic_unstemmed	misc MutChromSeq misc Gene cloning misc Mutational genomics misc Chromosome flow sorting misc Triticeae misc Wheat misc Barley
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title	Rapid gene isolation in barley and wheat by mutant chromosome sequencing
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title_full	Rapid gene isolation in barley and wheat by mutant chromosome sequencing
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author_browse	Sánchez-Martín, Javier Steuernagel, Burkhard Ghosh, Sreya Herren, Gerhard Hurni, Severine Adamski, Nikolai Vrána, Jan Kubaláková, Marie Krattinger, Simon G. Wicker, Thomas Doležel, Jaroslav Keller, Beat Wulff, Brande B. H.
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title_sort	rapid gene isolation in barley and wheat by mutant chromosome sequencing
title_auth	Rapid gene isolation in barley and wheat by mutant chromosome sequencing
abstract	Abstract Identification of causal mutations in barley and wheat is hampered by their large genomes and suppressed recombination. To overcome these obstacles, we have developed MutChromSeq, a complexity reduction approach based on flow sorting and sequencing of mutant chromosomes, to identify induced mutations by comparison to parental chromosomes. We apply MutChromSeq to six mutants each of the barley Eceriferum-q gene and the wheat Pm2 genes. This approach unambiguously identified single candidate genes that were verified by Sanger sequencing of additional mutants. MutChromSeq enables reference-free forward genetics in barley and wheat, thus opening up their pan-genomes to functional genomics. © The Author(s). 2016
abstractGer	Abstract Identification of causal mutations in barley and wheat is hampered by their large genomes and suppressed recombination. To overcome these obstacles, we have developed MutChromSeq, a complexity reduction approach based on flow sorting and sequencing of mutant chromosomes, to identify induced mutations by comparison to parental chromosomes. We apply MutChromSeq to six mutants each of the barley Eceriferum-q gene and the wheat Pm2 genes. This approach unambiguously identified single candidate genes that were verified by Sanger sequencing of additional mutants. MutChromSeq enables reference-free forward genetics in barley and wheat, thus opening up their pan-genomes to functional genomics. © The Author(s). 2016
abstract_unstemmed	Abstract Identification of causal mutations in barley and wheat is hampered by their large genomes and suppressed recombination. To overcome these obstacles, we have developed MutChromSeq, a complexity reduction approach based on flow sorting and sequencing of mutant chromosomes, to identify induced mutations by comparison to parental chromosomes. We apply MutChromSeq to six mutants each of the barley Eceriferum-q gene and the wheat Pm2 genes. This approach unambiguously identified single candidate genes that were verified by Sanger sequencing of additional mutants. MutChromSeq enables reference-free forward genetics in barley and wheat, thus opening up their pan-genomes to functional genomics. © The Author(s). 2016
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title_short	Rapid gene isolation in barley and wheat by mutant chromosome sequencing
url	https://dx.doi.org/10.1186/s13059-016-1082-1
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author2	Steuernagel, Burkhard Ghosh, Sreya Herren, Gerhard Hurni, Severine Adamski, Nikolai Vrána, Jan Kubaláková, Marie Krattinger, Simon G. Wicker, Thomas Doležel, Jaroslav Keller, Beat Wulff, Brande B. H.
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Rapid gene isolation in barley and wheat by mutant chromosome sequencing

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