Strain and bioprocess improvement of a thermophilic anaerobe for the production of ethanol from wood

Background The thermophilic, anaerobic bacterium Thermoanaerobacterium saccharolyticum digests hemicellulose and utilizes the major sugars present in biomass. It was previously engineered to produce ethanol at yields equivalent to yeast. While saccharolytic anaerobes have been long studied as potent...
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Autor*in:	Herring, Christopher D. [verfasserIn] Kenealy, William R. Joe Shaw, A. Covalla, Sean F. Olson, Daniel G. Zhang, Jiayi Ryan Sillers, W. Tsakraklides, Vasiliki Bardsley, John S. Rogers, Stephen R. Thorne, Philip G. Johnson, Jessica P. Foster, Abigail Shikhare, Indraneel D. Klingeman, Dawn M. Brown, Steven D. Davison, Brian H. Lynd, Lee R. Hogsett, David A.

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2016

Schlagwörter:	Cellulosic ethanol Consolidated bioprocessing Organism development Metabolic engineering Bioprocess development Thermophilic bacteria

Anmerkung:	© The Author(s) 2016

Übergeordnetes Werk:	Enthalten in: Biotechnology for biofuels - London : BioMed Central, 2008, 9(2016), 1 vom: 16. Juni
Übergeordnetes Werk:	volume:9 ; year:2016 ; number:1 ; day:16 ; month:06

Links:	Volltext

DOI / URN:	10.1186/s13068-016-0536-8

Katalog-ID:	SPR03014986X

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520			\|a Background The thermophilic, anaerobic bacterium Thermoanaerobacterium saccharolyticum digests hemicellulose and utilizes the major sugars present in biomass. It was previously engineered to produce ethanol at yields equivalent to yeast. While saccharolytic anaerobes have been long studied as potential biomass-fermenting organisms, development efforts for commercial ethanol production have not been reported. Results Here, we describe the highest ethanol titers achieved from T. saccharolyticum during a 4-year project to develop it for industrial production of ethanol from pre-treated hardwood at 51–55 °C. We describe organism and bioprocess development efforts undertaken to improve ethanol production. The final strain M2886 was generated by removing genes for exopolysaccharide synthesis, the regulator perR, and re-introduction of phosphotransacetylase and acetate kinase into the methyglyoxal synthase gene. It was also subject to multiple rounds of adaptation and selection, resulting in mutations later identified by resequencing. The highest ethanol titer achieved was 70 g/L in batch culture with a mixture of cellobiose and maltodextrin. In a “mock hydrolysate” Simultaneous Saccharification and Fermentation (SSF) with Sigmacell-20, glucose, xylose, and acetic acid, an ethanol titer of 61 g/L was achieved, at 92 % of theoretical yield. Fungal cellulases were rapidly inactivated under these conditions and had to be supplemented with cellulosomes from C. thermocellum. Ethanol titers of 31 g/L were reached in a 100 L SSF of pre-treated hardwood and 26 g/L in a fermentation of a hardwood hemicellulose extract. Conclusions This study demonstrates that thermophilic anaerobes are capable of producing ethanol at high yield and at titers greater than 60 g/L from purified substrates, but additional work is needed to produce the same ethanol titers from pre-treated hardwood.
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650		4	\|a Organism development \|7 (dpeaa)DE-He213
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700	1		\|a Tsakraklides, Vasiliki \|4 aut
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700	1		\|a Rogers, Stephen R. \|4 aut
700	1		\|a Thorne, Philip G. \|4 aut
700	1		\|a Johnson, Jessica P. \|4 aut
700	1		\|a Foster, Abigail \|4 aut
700	1		\|a Shikhare, Indraneel D. \|4 aut
700	1		\|a Klingeman, Dawn M. \|4 aut
700	1		\|a Brown, Steven D. \|4 aut
700	1		\|a Davison, Brian H. \|4 aut
700	1		\|a Lynd, Lee R. \|4 aut
700	1		\|a Hogsett, David A. \|4 aut
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allfields	10.1186/s13068-016-0536-8 doi (DE-627)SPR03014986X (SPR)s13068-016-0536-8-e DE-627 ger DE-627 rakwb eng Herring, Christopher D. verfasserin aut Strain and bioprocess improvement of a thermophilic anaerobe for the production of ethanol from wood 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2016 Background The thermophilic, anaerobic bacterium Thermoanaerobacterium saccharolyticum digests hemicellulose and utilizes the major sugars present in biomass. It was previously engineered to produce ethanol at yields equivalent to yeast. While saccharolytic anaerobes have been long studied as potential biomass-fermenting organisms, development efforts for commercial ethanol production have not been reported. Results Here, we describe the highest ethanol titers achieved from T. saccharolyticum during a 4-year project to develop it for industrial production of ethanol from pre-treated hardwood at 51–55 °C. We describe organism and bioprocess development efforts undertaken to improve ethanol production. The final strain M2886 was generated by removing genes for exopolysaccharide synthesis, the regulator perR, and re-introduction of phosphotransacetylase and acetate kinase into the methyglyoxal synthase gene. It was also subject to multiple rounds of adaptation and selection, resulting in mutations later identified by resequencing. The highest ethanol titer achieved was 70 g/L in batch culture with a mixture of cellobiose and maltodextrin. In a “mock hydrolysate” Simultaneous Saccharification and Fermentation (SSF) with Sigmacell-20, glucose, xylose, and acetic acid, an ethanol titer of 61 g/L was achieved, at 92 % of theoretical yield. Fungal cellulases were rapidly inactivated under these conditions and had to be supplemented with cellulosomes from C. thermocellum. Ethanol titers of 31 g/L were reached in a 100 L SSF of pre-treated hardwood and 26 g/L in a fermentation of a hardwood hemicellulose extract. Conclusions This study demonstrates that thermophilic anaerobes are capable of producing ethanol at high yield and at titers greater than 60 g/L from purified substrates, but additional work is needed to produce the same ethanol titers from pre-treated hardwood. Cellulosic ethanol (dpeaa)DE-He213 Consolidated bioprocessing (dpeaa)DE-He213 Organism development (dpeaa)DE-He213 Metabolic engineering (dpeaa)DE-He213 Bioprocess development (dpeaa)DE-He213 Thermophilic bacteria (dpeaa)DE-He213 Kenealy, William R. aut Joe Shaw, A. aut Covalla, Sean F. aut Olson, Daniel G. aut Zhang, Jiayi aut Ryan Sillers, W. aut Tsakraklides, Vasiliki aut Bardsley, John S. aut Rogers, Stephen R. aut Thorne, Philip G. aut Johnson, Jessica P. aut Foster, Abigail aut Shikhare, Indraneel D. aut Klingeman, Dawn M. aut Brown, Steven D. aut Davison, Brian H. aut Lynd, Lee R. aut Hogsett, David A. aut Enthalten in Biotechnology for biofuels London : BioMed Central, 2008 9(2016), 1 vom: 16. Juni (DE-627)563167882 (DE-600)2421351-2 1754-6834 nnns volume:9 year:2016 number:1 day:16 month:06 https://dx.doi.org/10.1186/s13068-016-0536-8 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2027 GBV_ILN_2055 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2119 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2016 1 16 06
spelling	10.1186/s13068-016-0536-8 doi (DE-627)SPR03014986X (SPR)s13068-016-0536-8-e DE-627 ger DE-627 rakwb eng Herring, Christopher D. verfasserin aut Strain and bioprocess improvement of a thermophilic anaerobe for the production of ethanol from wood 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2016 Background The thermophilic, anaerobic bacterium Thermoanaerobacterium saccharolyticum digests hemicellulose and utilizes the major sugars present in biomass. It was previously engineered to produce ethanol at yields equivalent to yeast. While saccharolytic anaerobes have been long studied as potential biomass-fermenting organisms, development efforts for commercial ethanol production have not been reported. Results Here, we describe the highest ethanol titers achieved from T. saccharolyticum during a 4-year project to develop it for industrial production of ethanol from pre-treated hardwood at 51–55 °C. We describe organism and bioprocess development efforts undertaken to improve ethanol production. The final strain M2886 was generated by removing genes for exopolysaccharide synthesis, the regulator perR, and re-introduction of phosphotransacetylase and acetate kinase into the methyglyoxal synthase gene. It was also subject to multiple rounds of adaptation and selection, resulting in mutations later identified by resequencing. The highest ethanol titer achieved was 70 g/L in batch culture with a mixture of cellobiose and maltodextrin. In a “mock hydrolysate” Simultaneous Saccharification and Fermentation (SSF) with Sigmacell-20, glucose, xylose, and acetic acid, an ethanol titer of 61 g/L was achieved, at 92 % of theoretical yield. Fungal cellulases were rapidly inactivated under these conditions and had to be supplemented with cellulosomes from C. thermocellum. Ethanol titers of 31 g/L were reached in a 100 L SSF of pre-treated hardwood and 26 g/L in a fermentation of a hardwood hemicellulose extract. Conclusions This study demonstrates that thermophilic anaerobes are capable of producing ethanol at high yield and at titers greater than 60 g/L from purified substrates, but additional work is needed to produce the same ethanol titers from pre-treated hardwood. Cellulosic ethanol (dpeaa)DE-He213 Consolidated bioprocessing (dpeaa)DE-He213 Organism development (dpeaa)DE-He213 Metabolic engineering (dpeaa)DE-He213 Bioprocess development (dpeaa)DE-He213 Thermophilic bacteria (dpeaa)DE-He213 Kenealy, William R. aut Joe Shaw, A. aut Covalla, Sean F. aut Olson, Daniel G. aut Zhang, Jiayi aut Ryan Sillers, W. aut Tsakraklides, Vasiliki aut Bardsley, John S. aut Rogers, Stephen R. aut Thorne, Philip G. aut Johnson, Jessica P. aut Foster, Abigail aut Shikhare, Indraneel D. aut Klingeman, Dawn M. aut Brown, Steven D. aut Davison, Brian H. aut Lynd, Lee R. aut Hogsett, David A. aut Enthalten in Biotechnology for biofuels London : BioMed Central, 2008 9(2016), 1 vom: 16. Juni (DE-627)563167882 (DE-600)2421351-2 1754-6834 nnns volume:9 year:2016 number:1 day:16 month:06 https://dx.doi.org/10.1186/s13068-016-0536-8 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2027 GBV_ILN_2055 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2119 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2016 1 16 06
allfields_unstemmed	10.1186/s13068-016-0536-8 doi (DE-627)SPR03014986X (SPR)s13068-016-0536-8-e DE-627 ger DE-627 rakwb eng Herring, Christopher D. verfasserin aut Strain and bioprocess improvement of a thermophilic anaerobe for the production of ethanol from wood 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2016 Background The thermophilic, anaerobic bacterium Thermoanaerobacterium saccharolyticum digests hemicellulose and utilizes the major sugars present in biomass. It was previously engineered to produce ethanol at yields equivalent to yeast. While saccharolytic anaerobes have been long studied as potential biomass-fermenting organisms, development efforts for commercial ethanol production have not been reported. Results Here, we describe the highest ethanol titers achieved from T. saccharolyticum during a 4-year project to develop it for industrial production of ethanol from pre-treated hardwood at 51–55 °C. We describe organism and bioprocess development efforts undertaken to improve ethanol production. The final strain M2886 was generated by removing genes for exopolysaccharide synthesis, the regulator perR, and re-introduction of phosphotransacetylase and acetate kinase into the methyglyoxal synthase gene. It was also subject to multiple rounds of adaptation and selection, resulting in mutations later identified by resequencing. The highest ethanol titer achieved was 70 g/L in batch culture with a mixture of cellobiose and maltodextrin. In a “mock hydrolysate” Simultaneous Saccharification and Fermentation (SSF) with Sigmacell-20, glucose, xylose, and acetic acid, an ethanol titer of 61 g/L was achieved, at 92 % of theoretical yield. Fungal cellulases were rapidly inactivated under these conditions and had to be supplemented with cellulosomes from C. thermocellum. Ethanol titers of 31 g/L were reached in a 100 L SSF of pre-treated hardwood and 26 g/L in a fermentation of a hardwood hemicellulose extract. Conclusions This study demonstrates that thermophilic anaerobes are capable of producing ethanol at high yield and at titers greater than 60 g/L from purified substrates, but additional work is needed to produce the same ethanol titers from pre-treated hardwood. Cellulosic ethanol (dpeaa)DE-He213 Consolidated bioprocessing (dpeaa)DE-He213 Organism development (dpeaa)DE-He213 Metabolic engineering (dpeaa)DE-He213 Bioprocess development (dpeaa)DE-He213 Thermophilic bacteria (dpeaa)DE-He213 Kenealy, William R. aut Joe Shaw, A. aut Covalla, Sean F. aut Olson, Daniel G. aut Zhang, Jiayi aut Ryan Sillers, W. aut Tsakraklides, Vasiliki aut Bardsley, John S. aut Rogers, Stephen R. aut Thorne, Philip G. aut Johnson, Jessica P. aut Foster, Abigail aut Shikhare, Indraneel D. aut Klingeman, Dawn M. aut Brown, Steven D. aut Davison, Brian H. aut Lynd, Lee R. aut Hogsett, David A. aut Enthalten in Biotechnology for biofuels London : BioMed Central, 2008 9(2016), 1 vom: 16. Juni (DE-627)563167882 (DE-600)2421351-2 1754-6834 nnns volume:9 year:2016 number:1 day:16 month:06 https://dx.doi.org/10.1186/s13068-016-0536-8 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2027 GBV_ILN_2055 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2119 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2016 1 16 06
allfieldsGer	10.1186/s13068-016-0536-8 doi (DE-627)SPR03014986X (SPR)s13068-016-0536-8-e DE-627 ger DE-627 rakwb eng Herring, Christopher D. verfasserin aut Strain and bioprocess improvement of a thermophilic anaerobe for the production of ethanol from wood 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2016 Background The thermophilic, anaerobic bacterium Thermoanaerobacterium saccharolyticum digests hemicellulose and utilizes the major sugars present in biomass. It was previously engineered to produce ethanol at yields equivalent to yeast. While saccharolytic anaerobes have been long studied as potential biomass-fermenting organisms, development efforts for commercial ethanol production have not been reported. Results Here, we describe the highest ethanol titers achieved from T. saccharolyticum during a 4-year project to develop it for industrial production of ethanol from pre-treated hardwood at 51–55 °C. We describe organism and bioprocess development efforts undertaken to improve ethanol production. The final strain M2886 was generated by removing genes for exopolysaccharide synthesis, the regulator perR, and re-introduction of phosphotransacetylase and acetate kinase into the methyglyoxal synthase gene. It was also subject to multiple rounds of adaptation and selection, resulting in mutations later identified by resequencing. The highest ethanol titer achieved was 70 g/L in batch culture with a mixture of cellobiose and maltodextrin. In a “mock hydrolysate” Simultaneous Saccharification and Fermentation (SSF) with Sigmacell-20, glucose, xylose, and acetic acid, an ethanol titer of 61 g/L was achieved, at 92 % of theoretical yield. Fungal cellulases were rapidly inactivated under these conditions and had to be supplemented with cellulosomes from C. thermocellum. Ethanol titers of 31 g/L were reached in a 100 L SSF of pre-treated hardwood and 26 g/L in a fermentation of a hardwood hemicellulose extract. Conclusions This study demonstrates that thermophilic anaerobes are capable of producing ethanol at high yield and at titers greater than 60 g/L from purified substrates, but additional work is needed to produce the same ethanol titers from pre-treated hardwood. Cellulosic ethanol (dpeaa)DE-He213 Consolidated bioprocessing (dpeaa)DE-He213 Organism development (dpeaa)DE-He213 Metabolic engineering (dpeaa)DE-He213 Bioprocess development (dpeaa)DE-He213 Thermophilic bacteria (dpeaa)DE-He213 Kenealy, William R. aut Joe Shaw, A. aut Covalla, Sean F. aut Olson, Daniel G. aut Zhang, Jiayi aut Ryan Sillers, W. aut Tsakraklides, Vasiliki aut Bardsley, John S. aut Rogers, Stephen R. aut Thorne, Philip G. aut Johnson, Jessica P. aut Foster, Abigail aut Shikhare, Indraneel D. aut Klingeman, Dawn M. aut Brown, Steven D. aut Davison, Brian H. aut Lynd, Lee R. aut Hogsett, David A. aut Enthalten in Biotechnology for biofuels London : BioMed Central, 2008 9(2016), 1 vom: 16. Juni (DE-627)563167882 (DE-600)2421351-2 1754-6834 nnns volume:9 year:2016 number:1 day:16 month:06 https://dx.doi.org/10.1186/s13068-016-0536-8 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2027 GBV_ILN_2055 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2119 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2016 1 16 06
allfieldsSound	10.1186/s13068-016-0536-8 doi (DE-627)SPR03014986X (SPR)s13068-016-0536-8-e DE-627 ger DE-627 rakwb eng Herring, Christopher D. verfasserin aut Strain and bioprocess improvement of a thermophilic anaerobe for the production of ethanol from wood 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2016 Background The thermophilic, anaerobic bacterium Thermoanaerobacterium saccharolyticum digests hemicellulose and utilizes the major sugars present in biomass. It was previously engineered to produce ethanol at yields equivalent to yeast. While saccharolytic anaerobes have been long studied as potential biomass-fermenting organisms, development efforts for commercial ethanol production have not been reported. Results Here, we describe the highest ethanol titers achieved from T. saccharolyticum during a 4-year project to develop it for industrial production of ethanol from pre-treated hardwood at 51–55 °C. We describe organism and bioprocess development efforts undertaken to improve ethanol production. The final strain M2886 was generated by removing genes for exopolysaccharide synthesis, the regulator perR, and re-introduction of phosphotransacetylase and acetate kinase into the methyglyoxal synthase gene. It was also subject to multiple rounds of adaptation and selection, resulting in mutations later identified by resequencing. The highest ethanol titer achieved was 70 g/L in batch culture with a mixture of cellobiose and maltodextrin. In a “mock hydrolysate” Simultaneous Saccharification and Fermentation (SSF) with Sigmacell-20, glucose, xylose, and acetic acid, an ethanol titer of 61 g/L was achieved, at 92 % of theoretical yield. Fungal cellulases were rapidly inactivated under these conditions and had to be supplemented with cellulosomes from C. thermocellum. Ethanol titers of 31 g/L were reached in a 100 L SSF of pre-treated hardwood and 26 g/L in a fermentation of a hardwood hemicellulose extract. Conclusions This study demonstrates that thermophilic anaerobes are capable of producing ethanol at high yield and at titers greater than 60 g/L from purified substrates, but additional work is needed to produce the same ethanol titers from pre-treated hardwood. Cellulosic ethanol (dpeaa)DE-He213 Consolidated bioprocessing (dpeaa)DE-He213 Organism development (dpeaa)DE-He213 Metabolic engineering (dpeaa)DE-He213 Bioprocess development (dpeaa)DE-He213 Thermophilic bacteria (dpeaa)DE-He213 Kenealy, William R. aut Joe Shaw, A. aut Covalla, Sean F. aut Olson, Daniel G. aut Zhang, Jiayi aut Ryan Sillers, W. aut Tsakraklides, Vasiliki aut Bardsley, John S. aut Rogers, Stephen R. aut Thorne, Philip G. aut Johnson, Jessica P. aut Foster, Abigail aut Shikhare, Indraneel D. aut Klingeman, Dawn M. aut Brown, Steven D. aut Davison, Brian H. aut Lynd, Lee R. aut Hogsett, David A. aut Enthalten in Biotechnology for biofuels London : BioMed Central, 2008 9(2016), 1 vom: 16. Juni (DE-627)563167882 (DE-600)2421351-2 1754-6834 nnns volume:9 year:2016 number:1 day:16 month:06 https://dx.doi.org/10.1186/s13068-016-0536-8 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2027 GBV_ILN_2055 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2119 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2016 1 16 06
language	English
source	Enthalten in Biotechnology for biofuels 9(2016), 1 vom: 16. Juni volume:9 year:2016 number:1 day:16 month:06
sourceStr	Enthalten in Biotechnology for biofuels 9(2016), 1 vom: 16. Juni volume:9 year:2016 number:1 day:16 month:06
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Cellulosic ethanol Consolidated bioprocessing Organism development Metabolic engineering Bioprocess development Thermophilic bacteria
isfreeaccess_bool	true
container_title	Biotechnology for biofuels
authorswithroles_txt_mv	Herring, Christopher D. @@aut@@ Kenealy, William R. @@aut@@ Joe Shaw, A. @@aut@@ Covalla, Sean F. @@aut@@ Olson, Daniel G. @@aut@@ Zhang, Jiayi @@aut@@ Ryan Sillers, W. @@aut@@ Tsakraklides, Vasiliki @@aut@@ Bardsley, John S. @@aut@@ Rogers, Stephen R. @@aut@@ Thorne, Philip G. @@aut@@ Johnson, Jessica P. @@aut@@ Foster, Abigail @@aut@@ Shikhare, Indraneel D. @@aut@@ Klingeman, Dawn M. @@aut@@ Brown, Steven D. @@aut@@ Davison, Brian H. @@aut@@ Lynd, Lee R. @@aut@@ Hogsett, David A. @@aut@@
publishDateDaySort_date	2016-06-16T00:00:00Z
hierarchy_top_id	563167882
id	SPR03014986X
language_de	englisch
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It was previously engineered to produce ethanol at yields equivalent to yeast. While saccharolytic anaerobes have been long studied as potential biomass-fermenting organisms, development efforts for commercial ethanol production have not been reported. Results Here, we describe the highest ethanol titers achieved from T. saccharolyticum during a 4-year project to develop it for industrial production of ethanol from pre-treated hardwood at 51–55 °C. We describe organism and bioprocess development efforts undertaken to improve ethanol production. The final strain M2886 was generated by removing genes for exopolysaccharide synthesis, the regulator perR, and re-introduction of phosphotransacetylase and acetate kinase into the methyglyoxal synthase gene. It was also subject to multiple rounds of adaptation and selection, resulting in mutations later identified by resequencing. The highest ethanol titer achieved was 70 g/L in batch culture with a mixture of cellobiose and maltodextrin. In a “mock hydrolysate” Simultaneous Saccharification and Fermentation (SSF) with Sigmacell-20, glucose, xylose, and acetic acid, an ethanol titer of 61 g/L was achieved, at 92 % of theoretical yield. Fungal cellulases were rapidly inactivated under these conditions and had to be supplemented with cellulosomes from C. thermocellum. Ethanol titers of 31 g/L were reached in a 100 L SSF of pre-treated hardwood and 26 g/L in a fermentation of a hardwood hemicellulose extract. 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author	Herring, Christopher D.
spellingShingle	Herring, Christopher D. misc Cellulosic ethanol misc Consolidated bioprocessing misc Organism development misc Metabolic engineering misc Bioprocess development misc Thermophilic bacteria Strain and bioprocess improvement of a thermophilic anaerobe for the production of ethanol from wood
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topic_title	Strain and bioprocess improvement of a thermophilic anaerobe for the production of ethanol from wood Cellulosic ethanol (dpeaa)DE-He213 Consolidated bioprocessing (dpeaa)DE-He213 Organism development (dpeaa)DE-He213 Metabolic engineering (dpeaa)DE-He213 Bioprocess development (dpeaa)DE-He213 Thermophilic bacteria (dpeaa)DE-He213
topic	misc Cellulosic ethanol misc Consolidated bioprocessing misc Organism development misc Metabolic engineering misc Bioprocess development misc Thermophilic bacteria
topic_unstemmed	misc Cellulosic ethanol misc Consolidated bioprocessing misc Organism development misc Metabolic engineering misc Bioprocess development misc Thermophilic bacteria
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title	Strain and bioprocess improvement of a thermophilic anaerobe for the production of ethanol from wood
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title_full	Strain and bioprocess improvement of a thermophilic anaerobe for the production of ethanol from wood
author_sort	Herring, Christopher D.
journal	Biotechnology for biofuels
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author_browse	Herring, Christopher D. Kenealy, William R. Joe Shaw, A. Covalla, Sean F. Olson, Daniel G. Zhang, Jiayi Ryan Sillers, W. Tsakraklides, Vasiliki Bardsley, John S. Rogers, Stephen R. Thorne, Philip G. Johnson, Jessica P. Foster, Abigail Shikhare, Indraneel D. Klingeman, Dawn M. Brown, Steven D. Davison, Brian H. Lynd, Lee R. Hogsett, David A.
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author-letter	Herring, Christopher D.
doi_str_mv	10.1186/s13068-016-0536-8
title_sort	strain and bioprocess improvement of a thermophilic anaerobe for the production of ethanol from wood
title_auth	Strain and bioprocess improvement of a thermophilic anaerobe for the production of ethanol from wood
abstract	Background The thermophilic, anaerobic bacterium Thermoanaerobacterium saccharolyticum digests hemicellulose and utilizes the major sugars present in biomass. It was previously engineered to produce ethanol at yields equivalent to yeast. While saccharolytic anaerobes have been long studied as potential biomass-fermenting organisms, development efforts for commercial ethanol production have not been reported. Results Here, we describe the highest ethanol titers achieved from T. saccharolyticum during a 4-year project to develop it for industrial production of ethanol from pre-treated hardwood at 51–55 °C. We describe organism and bioprocess development efforts undertaken to improve ethanol production. The final strain M2886 was generated by removing genes for exopolysaccharide synthesis, the regulator perR, and re-introduction of phosphotransacetylase and acetate kinase into the methyglyoxal synthase gene. It was also subject to multiple rounds of adaptation and selection, resulting in mutations later identified by resequencing. The highest ethanol titer achieved was 70 g/L in batch culture with a mixture of cellobiose and maltodextrin. In a “mock hydrolysate” Simultaneous Saccharification and Fermentation (SSF) with Sigmacell-20, glucose, xylose, and acetic acid, an ethanol titer of 61 g/L was achieved, at 92 % of theoretical yield. Fungal cellulases were rapidly inactivated under these conditions and had to be supplemented with cellulosomes from C. thermocellum. Ethanol titers of 31 g/L were reached in a 100 L SSF of pre-treated hardwood and 26 g/L in a fermentation of a hardwood hemicellulose extract. Conclusions This study demonstrates that thermophilic anaerobes are capable of producing ethanol at high yield and at titers greater than 60 g/L from purified substrates, but additional work is needed to produce the same ethanol titers from pre-treated hardwood. © The Author(s) 2016
abstractGer	Background The thermophilic, anaerobic bacterium Thermoanaerobacterium saccharolyticum digests hemicellulose and utilizes the major sugars present in biomass. It was previously engineered to produce ethanol at yields equivalent to yeast. While saccharolytic anaerobes have been long studied as potential biomass-fermenting organisms, development efforts for commercial ethanol production have not been reported. Results Here, we describe the highest ethanol titers achieved from T. saccharolyticum during a 4-year project to develop it for industrial production of ethanol from pre-treated hardwood at 51–55 °C. We describe organism and bioprocess development efforts undertaken to improve ethanol production. The final strain M2886 was generated by removing genes for exopolysaccharide synthesis, the regulator perR, and re-introduction of phosphotransacetylase and acetate kinase into the methyglyoxal synthase gene. It was also subject to multiple rounds of adaptation and selection, resulting in mutations later identified by resequencing. The highest ethanol titer achieved was 70 g/L in batch culture with a mixture of cellobiose and maltodextrin. In a “mock hydrolysate” Simultaneous Saccharification and Fermentation (SSF) with Sigmacell-20, glucose, xylose, and acetic acid, an ethanol titer of 61 g/L was achieved, at 92 % of theoretical yield. Fungal cellulases were rapidly inactivated under these conditions and had to be supplemented with cellulosomes from C. thermocellum. Ethanol titers of 31 g/L were reached in a 100 L SSF of pre-treated hardwood and 26 g/L in a fermentation of a hardwood hemicellulose extract. Conclusions This study demonstrates that thermophilic anaerobes are capable of producing ethanol at high yield and at titers greater than 60 g/L from purified substrates, but additional work is needed to produce the same ethanol titers from pre-treated hardwood. © The Author(s) 2016
abstract_unstemmed	Background The thermophilic, anaerobic bacterium Thermoanaerobacterium saccharolyticum digests hemicellulose and utilizes the major sugars present in biomass. It was previously engineered to produce ethanol at yields equivalent to yeast. While saccharolytic anaerobes have been long studied as potential biomass-fermenting organisms, development efforts for commercial ethanol production have not been reported. Results Here, we describe the highest ethanol titers achieved from T. saccharolyticum during a 4-year project to develop it for industrial production of ethanol from pre-treated hardwood at 51–55 °C. We describe organism and bioprocess development efforts undertaken to improve ethanol production. The final strain M2886 was generated by removing genes for exopolysaccharide synthesis, the regulator perR, and re-introduction of phosphotransacetylase and acetate kinase into the methyglyoxal synthase gene. It was also subject to multiple rounds of adaptation and selection, resulting in mutations later identified by resequencing. The highest ethanol titer achieved was 70 g/L in batch culture with a mixture of cellobiose and maltodextrin. In a “mock hydrolysate” Simultaneous Saccharification and Fermentation (SSF) with Sigmacell-20, glucose, xylose, and acetic acid, an ethanol titer of 61 g/L was achieved, at 92 % of theoretical yield. Fungal cellulases were rapidly inactivated under these conditions and had to be supplemented with cellulosomes from C. thermocellum. Ethanol titers of 31 g/L were reached in a 100 L SSF of pre-treated hardwood and 26 g/L in a fermentation of a hardwood hemicellulose extract. Conclusions This study demonstrates that thermophilic anaerobes are capable of producing ethanol at high yield and at titers greater than 60 g/L from purified substrates, but additional work is needed to produce the same ethanol titers from pre-treated hardwood. © The Author(s) 2016
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title_short	Strain and bioprocess improvement of a thermophilic anaerobe for the production of ethanol from wood
url	https://dx.doi.org/10.1186/s13068-016-0536-8
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author2	Kenealy, William R. Joe Shaw, A. Covalla, Sean F. Olson, Daniel G. Zhang, Jiayi Ryan Sillers, W. Tsakraklides, Vasiliki Bardsley, John S. Rogers, Stephen R. Thorne, Philip G. Johnson, Jessica P. Foster, Abigail Shikhare, Indraneel D. Klingeman, Dawn M. Brown, Steven D. Davison, Brian H. Lynd, Lee R. Hogsett, David A.
author2Str	Kenealy, William R. Joe Shaw, A. Covalla, Sean F. Olson, Daniel G. Zhang, Jiayi Ryan Sillers, W. Tsakraklides, Vasiliki Bardsley, John S. Rogers, Stephen R. Thorne, Philip G. Johnson, Jessica P. Foster, Abigail Shikhare, Indraneel D. Klingeman, Dawn M. Brown, Steven D. Davison, Brian H. Lynd, Lee R. Hogsett, David A.
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up_date	2024-07-03T14:16:51.941Z
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Strain and bioprocess improvement of a thermophilic anaerobe for the production of ethanol from wood

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