Role of NF-kβ factor Rel2 during Plasmodium falciparum and bacterial infection in Anopheles dirus

Background Anopheles mosquitoes transmit malaria which is one of the world’s most threatening diseases. Anopheles dirus (sensu stricto) is among the main vectors of malaria in South East Asia. The mosquito innate immune response is the first line of defence against malaria parasites during its devel...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Khan, Mohammad Behram [verfasserIn] Liew, Jonathan Wee Kent Leong, Cherng Shii Lau, Yee-Ling

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2016

Schlagwörter:	IMD pathway Bacterial infection

Anmerkung:	© The Author(s). 2016

Übergeordnetes Werk:	Enthalten in: Parasites & vectors - London : BioMed Central, 2008, 9(2016), 1 vom: 29. Sept.
Übergeordnetes Werk:	volume:9 ; year:2016 ; number:1 ; day:29 ; month:09

Links:	Volltext

DOI / URN:	10.1186/s13071-016-1810-0

Katalog-ID:	SPR030240476

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520			\|a Background Anopheles mosquitoes transmit malaria which is one of the world’s most threatening diseases. Anopheles dirus (sensu stricto) is among the main vectors of malaria in South East Asia. The mosquito innate immune response is the first line of defence against malaria parasites during its development. The immune deficiency (IMD) pathway, a conserved immune signaling pathway, influences anti-Plasmodium falciparum activity in Anopheles gambiae, An. stephensi and An. albimanus. The aim of the study was to determine the role of Rel2, an IMD pathway-controlled NF-kappaβ transcription factor, in An. dirus. Methods RACE (Rapid amplification of cDNA ends) was performed on the Rel2 gene. Double-stranded Rel2 was constructed and injected into the thorax of female mosquitoes. The injected mosquitoes were fed on a P. falciparum gametocyte culture and dissected on day 7–9 post-feeding in order to count the oocysts. A survival analysis was conducted by exposing the dsRNA injected mosquitoes to Gram-positive and Gram-negative bacteria. Results This study demonstrated that the Rel2 gene in An. dirus has two isoforms, short length and full length. RNA interference-mediated gene silencing of Rel2 showed that the latter is involved in protection against P. falciparum, Gram-positive bacteria (Micrococcus luteus) with Lys-type peptidoglycan and Gram-negative bacteria (Escherichia coli) with DAP-type peptidoglycan. Conclusion This study suggested that there are similarities in the splicing events and functionality of the Rel2 gene, between the Anopheles species. Among all the important anophelines, the immunity of only a few has been thoroughly investigated. In order to develop novel vector-based control strategies and restrict malaria transmission, the immune pathways of these important vectors should be thoroughly investigated.
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allfields	10.1186/s13071-016-1810-0 doi (DE-627)SPR030240476 (SPR)s13071-016-1810-0-e DE-627 ger DE-627 rakwb eng Khan, Mohammad Behram verfasserin aut Role of NF-kβ factor Rel2 during Plasmodium falciparum and bacterial infection in Anopheles dirus 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2016 Background Anopheles mosquitoes transmit malaria which is one of the world’s most threatening diseases. Anopheles dirus (sensu stricto) is among the main vectors of malaria in South East Asia. The mosquito innate immune response is the first line of defence against malaria parasites during its development. The immune deficiency (IMD) pathway, a conserved immune signaling pathway, influences anti-Plasmodium falciparum activity in Anopheles gambiae, An. stephensi and An. albimanus. The aim of the study was to determine the role of Rel2, an IMD pathway-controlled NF-kappaβ transcription factor, in An. dirus. Methods RACE (Rapid amplification of cDNA ends) was performed on the Rel2 gene. Double-stranded Rel2 was constructed and injected into the thorax of female mosquitoes. The injected mosquitoes were fed on a P. falciparum gametocyte culture and dissected on day 7–9 post-feeding in order to count the oocysts. A survival analysis was conducted by exposing the dsRNA injected mosquitoes to Gram-positive and Gram-negative bacteria. Results This study demonstrated that the Rel2 gene in An. dirus has two isoforms, short length and full length. RNA interference-mediated gene silencing of Rel2 showed that the latter is involved in protection against P. falciparum, Gram-positive bacteria (Micrococcus luteus) with Lys-type peptidoglycan and Gram-negative bacteria (Escherichia coli) with DAP-type peptidoglycan. Conclusion This study suggested that there are similarities in the splicing events and functionality of the Rel2 gene, between the Anopheles species. Among all the important anophelines, the immunity of only a few has been thoroughly investigated. In order to develop novel vector-based control strategies and restrict malaria transmission, the immune pathways of these important vectors should be thoroughly investigated. IMD pathway (dpeaa)DE-He213 Bacterial infection (dpeaa)DE-He213 Liew, Jonathan Wee Kent aut Leong, Cherng Shii aut Lau, Yee-Ling aut Enthalten in Parasites & vectors London : BioMed Central, 2008 9(2016), 1 vom: 29. Sept. (DE-627)558690076 (DE-600)2409480-8 1756-3305 nnns volume:9 year:2016 number:1 day:29 month:09 https://dx.doi.org/10.1186/s13071-016-1810-0 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2016 1 29 09
spelling	10.1186/s13071-016-1810-0 doi (DE-627)SPR030240476 (SPR)s13071-016-1810-0-e DE-627 ger DE-627 rakwb eng Khan, Mohammad Behram verfasserin aut Role of NF-kβ factor Rel2 during Plasmodium falciparum and bacterial infection in Anopheles dirus 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2016 Background Anopheles mosquitoes transmit malaria which is one of the world’s most threatening diseases. Anopheles dirus (sensu stricto) is among the main vectors of malaria in South East Asia. The mosquito innate immune response is the first line of defence against malaria parasites during its development. The immune deficiency (IMD) pathway, a conserved immune signaling pathway, influences anti-Plasmodium falciparum activity in Anopheles gambiae, An. stephensi and An. albimanus. The aim of the study was to determine the role of Rel2, an IMD pathway-controlled NF-kappaβ transcription factor, in An. dirus. Methods RACE (Rapid amplification of cDNA ends) was performed on the Rel2 gene. Double-stranded Rel2 was constructed and injected into the thorax of female mosquitoes. The injected mosquitoes were fed on a P. falciparum gametocyte culture and dissected on day 7–9 post-feeding in order to count the oocysts. A survival analysis was conducted by exposing the dsRNA injected mosquitoes to Gram-positive and Gram-negative bacteria. Results This study demonstrated that the Rel2 gene in An. dirus has two isoforms, short length and full length. RNA interference-mediated gene silencing of Rel2 showed that the latter is involved in protection against P. falciparum, Gram-positive bacteria (Micrococcus luteus) with Lys-type peptidoglycan and Gram-negative bacteria (Escherichia coli) with DAP-type peptidoglycan. Conclusion This study suggested that there are similarities in the splicing events and functionality of the Rel2 gene, between the Anopheles species. Among all the important anophelines, the immunity of only a few has been thoroughly investigated. In order to develop novel vector-based control strategies and restrict malaria transmission, the immune pathways of these important vectors should be thoroughly investigated. IMD pathway (dpeaa)DE-He213 Bacterial infection (dpeaa)DE-He213 Liew, Jonathan Wee Kent aut Leong, Cherng Shii aut Lau, Yee-Ling aut Enthalten in Parasites & vectors London : BioMed Central, 2008 9(2016), 1 vom: 29. Sept. (DE-627)558690076 (DE-600)2409480-8 1756-3305 nnns volume:9 year:2016 number:1 day:29 month:09 https://dx.doi.org/10.1186/s13071-016-1810-0 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2016 1 29 09
allfields_unstemmed	10.1186/s13071-016-1810-0 doi (DE-627)SPR030240476 (SPR)s13071-016-1810-0-e DE-627 ger DE-627 rakwb eng Khan, Mohammad Behram verfasserin aut Role of NF-kβ factor Rel2 during Plasmodium falciparum and bacterial infection in Anopheles dirus 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2016 Background Anopheles mosquitoes transmit malaria which is one of the world’s most threatening diseases. Anopheles dirus (sensu stricto) is among the main vectors of malaria in South East Asia. The mosquito innate immune response is the first line of defence against malaria parasites during its development. The immune deficiency (IMD) pathway, a conserved immune signaling pathway, influences anti-Plasmodium falciparum activity in Anopheles gambiae, An. stephensi and An. albimanus. The aim of the study was to determine the role of Rel2, an IMD pathway-controlled NF-kappaβ transcription factor, in An. dirus. Methods RACE (Rapid amplification of cDNA ends) was performed on the Rel2 gene. Double-stranded Rel2 was constructed and injected into the thorax of female mosquitoes. The injected mosquitoes were fed on a P. falciparum gametocyte culture and dissected on day 7–9 post-feeding in order to count the oocysts. A survival analysis was conducted by exposing the dsRNA injected mosquitoes to Gram-positive and Gram-negative bacteria. Results This study demonstrated that the Rel2 gene in An. dirus has two isoforms, short length and full length. RNA interference-mediated gene silencing of Rel2 showed that the latter is involved in protection against P. falciparum, Gram-positive bacteria (Micrococcus luteus) with Lys-type peptidoglycan and Gram-negative bacteria (Escherichia coli) with DAP-type peptidoglycan. Conclusion This study suggested that there are similarities in the splicing events and functionality of the Rel2 gene, between the Anopheles species. Among all the important anophelines, the immunity of only a few has been thoroughly investigated. In order to develop novel vector-based control strategies and restrict malaria transmission, the immune pathways of these important vectors should be thoroughly investigated. IMD pathway (dpeaa)DE-He213 Bacterial infection (dpeaa)DE-He213 Liew, Jonathan Wee Kent aut Leong, Cherng Shii aut Lau, Yee-Ling aut Enthalten in Parasites & vectors London : BioMed Central, 2008 9(2016), 1 vom: 29. Sept. (DE-627)558690076 (DE-600)2409480-8 1756-3305 nnns volume:9 year:2016 number:1 day:29 month:09 https://dx.doi.org/10.1186/s13071-016-1810-0 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2016 1 29 09
allfieldsGer	10.1186/s13071-016-1810-0 doi (DE-627)SPR030240476 (SPR)s13071-016-1810-0-e DE-627 ger DE-627 rakwb eng Khan, Mohammad Behram verfasserin aut Role of NF-kβ factor Rel2 during Plasmodium falciparum and bacterial infection in Anopheles dirus 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2016 Background Anopheles mosquitoes transmit malaria which is one of the world’s most threatening diseases. Anopheles dirus (sensu stricto) is among the main vectors of malaria in South East Asia. The mosquito innate immune response is the first line of defence against malaria parasites during its development. The immune deficiency (IMD) pathway, a conserved immune signaling pathway, influences anti-Plasmodium falciparum activity in Anopheles gambiae, An. stephensi and An. albimanus. The aim of the study was to determine the role of Rel2, an IMD pathway-controlled NF-kappaβ transcription factor, in An. dirus. Methods RACE (Rapid amplification of cDNA ends) was performed on the Rel2 gene. Double-stranded Rel2 was constructed and injected into the thorax of female mosquitoes. The injected mosquitoes were fed on a P. falciparum gametocyte culture and dissected on day 7–9 post-feeding in order to count the oocysts. A survival analysis was conducted by exposing the dsRNA injected mosquitoes to Gram-positive and Gram-negative bacteria. Results This study demonstrated that the Rel2 gene in An. dirus has two isoforms, short length and full length. RNA interference-mediated gene silencing of Rel2 showed that the latter is involved in protection against P. falciparum, Gram-positive bacteria (Micrococcus luteus) with Lys-type peptidoglycan and Gram-negative bacteria (Escherichia coli) with DAP-type peptidoglycan. Conclusion This study suggested that there are similarities in the splicing events and functionality of the Rel2 gene, between the Anopheles species. Among all the important anophelines, the immunity of only a few has been thoroughly investigated. In order to develop novel vector-based control strategies and restrict malaria transmission, the immune pathways of these important vectors should be thoroughly investigated. IMD pathway (dpeaa)DE-He213 Bacterial infection (dpeaa)DE-He213 Liew, Jonathan Wee Kent aut Leong, Cherng Shii aut Lau, Yee-Ling aut Enthalten in Parasites & vectors London : BioMed Central, 2008 9(2016), 1 vom: 29. Sept. (DE-627)558690076 (DE-600)2409480-8 1756-3305 nnns volume:9 year:2016 number:1 day:29 month:09 https://dx.doi.org/10.1186/s13071-016-1810-0 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2016 1 29 09
allfieldsSound	10.1186/s13071-016-1810-0 doi (DE-627)SPR030240476 (SPR)s13071-016-1810-0-e DE-627 ger DE-627 rakwb eng Khan, Mohammad Behram verfasserin aut Role of NF-kβ factor Rel2 during Plasmodium falciparum and bacterial infection in Anopheles dirus 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2016 Background Anopheles mosquitoes transmit malaria which is one of the world’s most threatening diseases. Anopheles dirus (sensu stricto) is among the main vectors of malaria in South East Asia. The mosquito innate immune response is the first line of defence against malaria parasites during its development. The immune deficiency (IMD) pathway, a conserved immune signaling pathway, influences anti-Plasmodium falciparum activity in Anopheles gambiae, An. stephensi and An. albimanus. The aim of the study was to determine the role of Rel2, an IMD pathway-controlled NF-kappaβ transcription factor, in An. dirus. Methods RACE (Rapid amplification of cDNA ends) was performed on the Rel2 gene. Double-stranded Rel2 was constructed and injected into the thorax of female mosquitoes. The injected mosquitoes were fed on a P. falciparum gametocyte culture and dissected on day 7–9 post-feeding in order to count the oocysts. A survival analysis was conducted by exposing the dsRNA injected mosquitoes to Gram-positive and Gram-negative bacteria. Results This study demonstrated that the Rel2 gene in An. dirus has two isoforms, short length and full length. RNA interference-mediated gene silencing of Rel2 showed that the latter is involved in protection against P. falciparum, Gram-positive bacteria (Micrococcus luteus) with Lys-type peptidoglycan and Gram-negative bacteria (Escherichia coli) with DAP-type peptidoglycan. Conclusion This study suggested that there are similarities in the splicing events and functionality of the Rel2 gene, between the Anopheles species. Among all the important anophelines, the immunity of only a few has been thoroughly investigated. In order to develop novel vector-based control strategies and restrict malaria transmission, the immune pathways of these important vectors should be thoroughly investigated. IMD pathway (dpeaa)DE-He213 Bacterial infection (dpeaa)DE-He213 Liew, Jonathan Wee Kent aut Leong, Cherng Shii aut Lau, Yee-Ling aut Enthalten in Parasites & vectors London : BioMed Central, 2008 9(2016), 1 vom: 29. Sept. (DE-627)558690076 (DE-600)2409480-8 1756-3305 nnns volume:9 year:2016 number:1 day:29 month:09 https://dx.doi.org/10.1186/s13071-016-1810-0 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2016 1 29 09
language	English
source	Enthalten in Parasites & vectors 9(2016), 1 vom: 29. Sept. volume:9 year:2016 number:1 day:29 month:09
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Results This study demonstrated that the Rel2 gene in An. dirus has two isoforms, short length and full length. RNA interference-mediated gene silencing of Rel2 showed that the latter is involved in protection against P. falciparum, Gram-positive bacteria (Micrococcus luteus) with Lys-type peptidoglycan and Gram-negative bacteria (Escherichia coli) with DAP-type peptidoglycan. Conclusion This study suggested that there are similarities in the splicing events and functionality of the Rel2 gene, between the Anopheles species. Among all the important anophelines, the immunity of only a few has been thoroughly investigated. 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author	Khan, Mohammad Behram
spellingShingle	Khan, Mohammad Behram misc IMD pathway misc Bacterial infection Role of NF-kβ factor Rel2 during Plasmodium falciparum and bacterial infection in Anopheles dirus
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topic_title	Role of NF-kβ factor Rel2 during Plasmodium falciparum and bacterial infection in Anopheles dirus IMD pathway (dpeaa)DE-He213 Bacterial infection (dpeaa)DE-He213
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title	Role of NF-kβ factor Rel2 during Plasmodium falciparum and bacterial infection in Anopheles dirus
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title_full	Role of NF-kβ factor Rel2 during Plasmodium falciparum and bacterial infection in Anopheles dirus
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title_sort	role of nf-kβ factor rel2 during plasmodium falciparum and bacterial infection in anopheles dirus
title_auth	Role of NF-kβ factor Rel2 during Plasmodium falciparum and bacterial infection in Anopheles dirus
abstract	Background Anopheles mosquitoes transmit malaria which is one of the world’s most threatening diseases. Anopheles dirus (sensu stricto) is among the main vectors of malaria in South East Asia. The mosquito innate immune response is the first line of defence against malaria parasites during its development. The immune deficiency (IMD) pathway, a conserved immune signaling pathway, influences anti-Plasmodium falciparum activity in Anopheles gambiae, An. stephensi and An. albimanus. The aim of the study was to determine the role of Rel2, an IMD pathway-controlled NF-kappaβ transcription factor, in An. dirus. Methods RACE (Rapid amplification of cDNA ends) was performed on the Rel2 gene. Double-stranded Rel2 was constructed and injected into the thorax of female mosquitoes. The injected mosquitoes were fed on a P. falciparum gametocyte culture and dissected on day 7–9 post-feeding in order to count the oocysts. A survival analysis was conducted by exposing the dsRNA injected mosquitoes to Gram-positive and Gram-negative bacteria. Results This study demonstrated that the Rel2 gene in An. dirus has two isoforms, short length and full length. RNA interference-mediated gene silencing of Rel2 showed that the latter is involved in protection against P. falciparum, Gram-positive bacteria (Micrococcus luteus) with Lys-type peptidoglycan and Gram-negative bacteria (Escherichia coli) with DAP-type peptidoglycan. Conclusion This study suggested that there are similarities in the splicing events and functionality of the Rel2 gene, between the Anopheles species. Among all the important anophelines, the immunity of only a few has been thoroughly investigated. In order to develop novel vector-based control strategies and restrict malaria transmission, the immune pathways of these important vectors should be thoroughly investigated. © The Author(s). 2016
abstractGer	Background Anopheles mosquitoes transmit malaria which is one of the world’s most threatening diseases. Anopheles dirus (sensu stricto) is among the main vectors of malaria in South East Asia. The mosquito innate immune response is the first line of defence against malaria parasites during its development. The immune deficiency (IMD) pathway, a conserved immune signaling pathway, influences anti-Plasmodium falciparum activity in Anopheles gambiae, An. stephensi and An. albimanus. The aim of the study was to determine the role of Rel2, an IMD pathway-controlled NF-kappaβ transcription factor, in An. dirus. Methods RACE (Rapid amplification of cDNA ends) was performed on the Rel2 gene. Double-stranded Rel2 was constructed and injected into the thorax of female mosquitoes. The injected mosquitoes were fed on a P. falciparum gametocyte culture and dissected on day 7–9 post-feeding in order to count the oocysts. A survival analysis was conducted by exposing the dsRNA injected mosquitoes to Gram-positive and Gram-negative bacteria. Results This study demonstrated that the Rel2 gene in An. dirus has two isoforms, short length and full length. RNA interference-mediated gene silencing of Rel2 showed that the latter is involved in protection against P. falciparum, Gram-positive bacteria (Micrococcus luteus) with Lys-type peptidoglycan and Gram-negative bacteria (Escherichia coli) with DAP-type peptidoglycan. Conclusion This study suggested that there are similarities in the splicing events and functionality of the Rel2 gene, between the Anopheles species. Among all the important anophelines, the immunity of only a few has been thoroughly investigated. In order to develop novel vector-based control strategies and restrict malaria transmission, the immune pathways of these important vectors should be thoroughly investigated. © The Author(s). 2016
abstract_unstemmed	Background Anopheles mosquitoes transmit malaria which is one of the world’s most threatening diseases. Anopheles dirus (sensu stricto) is among the main vectors of malaria in South East Asia. The mosquito innate immune response is the first line of defence against malaria parasites during its development. The immune deficiency (IMD) pathway, a conserved immune signaling pathway, influences anti-Plasmodium falciparum activity in Anopheles gambiae, An. stephensi and An. albimanus. The aim of the study was to determine the role of Rel2, an IMD pathway-controlled NF-kappaβ transcription factor, in An. dirus. Methods RACE (Rapid amplification of cDNA ends) was performed on the Rel2 gene. Double-stranded Rel2 was constructed and injected into the thorax of female mosquitoes. The injected mosquitoes were fed on a P. falciparum gametocyte culture and dissected on day 7–9 post-feeding in order to count the oocysts. A survival analysis was conducted by exposing the dsRNA injected mosquitoes to Gram-positive and Gram-negative bacteria. Results This study demonstrated that the Rel2 gene in An. dirus has two isoforms, short length and full length. RNA interference-mediated gene silencing of Rel2 showed that the latter is involved in protection against P. falciparum, Gram-positive bacteria (Micrococcus luteus) with Lys-type peptidoglycan and Gram-negative bacteria (Escherichia coli) with DAP-type peptidoglycan. Conclusion This study suggested that there are similarities in the splicing events and functionality of the Rel2 gene, between the Anopheles species. Among all the important anophelines, the immunity of only a few has been thoroughly investigated. In order to develop novel vector-based control strategies and restrict malaria transmission, the immune pathways of these important vectors should be thoroughly investigated. © The Author(s). 2016
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title_short	Role of NF-kβ factor Rel2 during Plasmodium falciparum and bacterial infection in Anopheles dirus
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Role of NF-kβ factor Rel2 during Plasmodium falciparum and bacterial infection in Anopheles dirus

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