A simple, step-by-step dissection protocol for the rapid isolation of mouse dorsal root ganglia

Background The cell bodies of sensory neurons, which transmit information from the external environment to the spinal cord, can be found at all levels of the spinal column in paired structures called dorsal root ganglia (DRG). Rodent DRG neurons have long been studied in the laboratory to improve un...
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Autor*in:	Sleigh, James N. [verfasserIn] Weir, Greg A. Schiavo, Giampietro

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2016

Schlagwörter:	Dorsal root ganglia (DRG) Sensory neuron Peripheral neuropathy Primary culture

Anmerkung:	© Sleigh et al. 2016

Übergeordnetes Werk:	Enthalten in: BMC Research Notes - London, 2008, 9(2016), 1 vom: 11. Feb.
Übergeordnetes Werk:	volume:9 ; year:2016 ; number:1 ; day:11 ; month:02

Links:	Volltext

DOI / URN:	10.1186/s13104-016-1915-8

Katalog-ID:	SPR030314763

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520			\|a Background The cell bodies of sensory neurons, which transmit information from the external environment to the spinal cord, can be found at all levels of the spinal column in paired structures called dorsal root ganglia (DRG). Rodent DRG neurons have long been studied in the laboratory to improve understanding of sensory nerve development and function, and have been instrumental in determining mechanisms underlying pain and neurodegeneration in disorders of the peripheral nervous system. Here, we describe a simple, step-by-step protocol for the swift isolation of mouse DRG, which can be enzymatically dissociated to produce fully differentiated primary neuronal cultures, or processed for downstream analyses, such as immunohistochemistry or RNA profiling. Findings After dissecting out the spinal column, from the base of the skull to the level of the femurs, it can be cut down the mid-line and the spinal cord and meninges removed, before extracting the DRG and detaching unwanted axons. This protocol allows the easy and rapid isolation of DRG with minimal practice and dissection experience. The process is both faster and less technically challenging than extracting the ganglia from the in situ column after performing a dorsal laminectomy. Conclusions This approach reduces the time required to collect DRG, thereby improving efficiency, permitting less opportunity for tissue deterioration, and, ultimately, increasing the chances of generating healthy primary DRG cultures or high quality, reproducible experiments using DRG tissue.
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allfields	10.1186/s13104-016-1915-8 doi (DE-627)SPR030314763 (SPR)s13104-016-1915-8-e DE-627 ger DE-627 rakwb eng Sleigh, James N. verfasserin aut A simple, step-by-step dissection protocol for the rapid isolation of mouse dorsal root ganglia 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Sleigh et al. 2016 Background The cell bodies of sensory neurons, which transmit information from the external environment to the spinal cord, can be found at all levels of the spinal column in paired structures called dorsal root ganglia (DRG). Rodent DRG neurons have long been studied in the laboratory to improve understanding of sensory nerve development and function, and have been instrumental in determining mechanisms underlying pain and neurodegeneration in disorders of the peripheral nervous system. Here, we describe a simple, step-by-step protocol for the swift isolation of mouse DRG, which can be enzymatically dissociated to produce fully differentiated primary neuronal cultures, or processed for downstream analyses, such as immunohistochemistry or RNA profiling. Findings After dissecting out the spinal column, from the base of the skull to the level of the femurs, it can be cut down the mid-line and the spinal cord and meninges removed, before extracting the DRG and detaching unwanted axons. This protocol allows the easy and rapid isolation of DRG with minimal practice and dissection experience. The process is both faster and less technically challenging than extracting the ganglia from the in situ column after performing a dorsal laminectomy. Conclusions This approach reduces the time required to collect DRG, thereby improving efficiency, permitting less opportunity for tissue deterioration, and, ultimately, increasing the chances of generating healthy primary DRG cultures or high quality, reproducible experiments using DRG tissue. Dorsal root ganglia (DRG) (dpeaa)DE-He213 Sensory neuron (dpeaa)DE-He213 Peripheral neuropathy (dpeaa)DE-He213 Primary culture (dpeaa)DE-He213 Weir, Greg A. aut Schiavo, Giampietro aut Enthalten in BMC Research Notes London, 2008 9(2016), 1 vom: 11. Feb. (DE-627)559431805 (DE-600)2413336-X 1756-0500 nnns volume:9 year:2016 number:1 day:11 month:02 https://dx.doi.org/10.1186/s13104-016-1915-8 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2016 1 11 02
spelling	10.1186/s13104-016-1915-8 doi (DE-627)SPR030314763 (SPR)s13104-016-1915-8-e DE-627 ger DE-627 rakwb eng Sleigh, James N. verfasserin aut A simple, step-by-step dissection protocol for the rapid isolation of mouse dorsal root ganglia 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Sleigh et al. 2016 Background The cell bodies of sensory neurons, which transmit information from the external environment to the spinal cord, can be found at all levels of the spinal column in paired structures called dorsal root ganglia (DRG). Rodent DRG neurons have long been studied in the laboratory to improve understanding of sensory nerve development and function, and have been instrumental in determining mechanisms underlying pain and neurodegeneration in disorders of the peripheral nervous system. Here, we describe a simple, step-by-step protocol for the swift isolation of mouse DRG, which can be enzymatically dissociated to produce fully differentiated primary neuronal cultures, or processed for downstream analyses, such as immunohistochemistry or RNA profiling. Findings After dissecting out the spinal column, from the base of the skull to the level of the femurs, it can be cut down the mid-line and the spinal cord and meninges removed, before extracting the DRG and detaching unwanted axons. This protocol allows the easy and rapid isolation of DRG with minimal practice and dissection experience. The process is both faster and less technically challenging than extracting the ganglia from the in situ column after performing a dorsal laminectomy. Conclusions This approach reduces the time required to collect DRG, thereby improving efficiency, permitting less opportunity for tissue deterioration, and, ultimately, increasing the chances of generating healthy primary DRG cultures or high quality, reproducible experiments using DRG tissue. Dorsal root ganglia (DRG) (dpeaa)DE-He213 Sensory neuron (dpeaa)DE-He213 Peripheral neuropathy (dpeaa)DE-He213 Primary culture (dpeaa)DE-He213 Weir, Greg A. aut Schiavo, Giampietro aut Enthalten in BMC Research Notes London, 2008 9(2016), 1 vom: 11. Feb. (DE-627)559431805 (DE-600)2413336-X 1756-0500 nnns volume:9 year:2016 number:1 day:11 month:02 https://dx.doi.org/10.1186/s13104-016-1915-8 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2016 1 11 02
allfields_unstemmed	10.1186/s13104-016-1915-8 doi (DE-627)SPR030314763 (SPR)s13104-016-1915-8-e DE-627 ger DE-627 rakwb eng Sleigh, James N. verfasserin aut A simple, step-by-step dissection protocol for the rapid isolation of mouse dorsal root ganglia 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Sleigh et al. 2016 Background The cell bodies of sensory neurons, which transmit information from the external environment to the spinal cord, can be found at all levels of the spinal column in paired structures called dorsal root ganglia (DRG). Rodent DRG neurons have long been studied in the laboratory to improve understanding of sensory nerve development and function, and have been instrumental in determining mechanisms underlying pain and neurodegeneration in disorders of the peripheral nervous system. Here, we describe a simple, step-by-step protocol for the swift isolation of mouse DRG, which can be enzymatically dissociated to produce fully differentiated primary neuronal cultures, or processed for downstream analyses, such as immunohistochemistry or RNA profiling. Findings After dissecting out the spinal column, from the base of the skull to the level of the femurs, it can be cut down the mid-line and the spinal cord and meninges removed, before extracting the DRG and detaching unwanted axons. This protocol allows the easy and rapid isolation of DRG with minimal practice and dissection experience. The process is both faster and less technically challenging than extracting the ganglia from the in situ column after performing a dorsal laminectomy. Conclusions This approach reduces the time required to collect DRG, thereby improving efficiency, permitting less opportunity for tissue deterioration, and, ultimately, increasing the chances of generating healthy primary DRG cultures or high quality, reproducible experiments using DRG tissue. Dorsal root ganglia (DRG) (dpeaa)DE-He213 Sensory neuron (dpeaa)DE-He213 Peripheral neuropathy (dpeaa)DE-He213 Primary culture (dpeaa)DE-He213 Weir, Greg A. aut Schiavo, Giampietro aut Enthalten in BMC Research Notes London, 2008 9(2016), 1 vom: 11. Feb. (DE-627)559431805 (DE-600)2413336-X 1756-0500 nnns volume:9 year:2016 number:1 day:11 month:02 https://dx.doi.org/10.1186/s13104-016-1915-8 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2016 1 11 02
allfieldsGer	10.1186/s13104-016-1915-8 doi (DE-627)SPR030314763 (SPR)s13104-016-1915-8-e DE-627 ger DE-627 rakwb eng Sleigh, James N. verfasserin aut A simple, step-by-step dissection protocol for the rapid isolation of mouse dorsal root ganglia 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Sleigh et al. 2016 Background The cell bodies of sensory neurons, which transmit information from the external environment to the spinal cord, can be found at all levels of the spinal column in paired structures called dorsal root ganglia (DRG). Rodent DRG neurons have long been studied in the laboratory to improve understanding of sensory nerve development and function, and have been instrumental in determining mechanisms underlying pain and neurodegeneration in disorders of the peripheral nervous system. Here, we describe a simple, step-by-step protocol for the swift isolation of mouse DRG, which can be enzymatically dissociated to produce fully differentiated primary neuronal cultures, or processed for downstream analyses, such as immunohistochemistry or RNA profiling. Findings After dissecting out the spinal column, from the base of the skull to the level of the femurs, it can be cut down the mid-line and the spinal cord and meninges removed, before extracting the DRG and detaching unwanted axons. This protocol allows the easy and rapid isolation of DRG with minimal practice and dissection experience. The process is both faster and less technically challenging than extracting the ganglia from the in situ column after performing a dorsal laminectomy. Conclusions This approach reduces the time required to collect DRG, thereby improving efficiency, permitting less opportunity for tissue deterioration, and, ultimately, increasing the chances of generating healthy primary DRG cultures or high quality, reproducible experiments using DRG tissue. Dorsal root ganglia (DRG) (dpeaa)DE-He213 Sensory neuron (dpeaa)DE-He213 Peripheral neuropathy (dpeaa)DE-He213 Primary culture (dpeaa)DE-He213 Weir, Greg A. aut Schiavo, Giampietro aut Enthalten in BMC Research Notes London, 2008 9(2016), 1 vom: 11. Feb. (DE-627)559431805 (DE-600)2413336-X 1756-0500 nnns volume:9 year:2016 number:1 day:11 month:02 https://dx.doi.org/10.1186/s13104-016-1915-8 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2016 1 11 02
allfieldsSound	10.1186/s13104-016-1915-8 doi (DE-627)SPR030314763 (SPR)s13104-016-1915-8-e DE-627 ger DE-627 rakwb eng Sleigh, James N. verfasserin aut A simple, step-by-step dissection protocol for the rapid isolation of mouse dorsal root ganglia 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Sleigh et al. 2016 Background The cell bodies of sensory neurons, which transmit information from the external environment to the spinal cord, can be found at all levels of the spinal column in paired structures called dorsal root ganglia (DRG). Rodent DRG neurons have long been studied in the laboratory to improve understanding of sensory nerve development and function, and have been instrumental in determining mechanisms underlying pain and neurodegeneration in disorders of the peripheral nervous system. Here, we describe a simple, step-by-step protocol for the swift isolation of mouse DRG, which can be enzymatically dissociated to produce fully differentiated primary neuronal cultures, or processed for downstream analyses, such as immunohistochemistry or RNA profiling. Findings After dissecting out the spinal column, from the base of the skull to the level of the femurs, it can be cut down the mid-line and the spinal cord and meninges removed, before extracting the DRG and detaching unwanted axons. This protocol allows the easy and rapid isolation of DRG with minimal practice and dissection experience. The process is both faster and less technically challenging than extracting the ganglia from the in situ column after performing a dorsal laminectomy. Conclusions This approach reduces the time required to collect DRG, thereby improving efficiency, permitting less opportunity for tissue deterioration, and, ultimately, increasing the chances of generating healthy primary DRG cultures or high quality, reproducible experiments using DRG tissue. Dorsal root ganglia (DRG) (dpeaa)DE-He213 Sensory neuron (dpeaa)DE-He213 Peripheral neuropathy (dpeaa)DE-He213 Primary culture (dpeaa)DE-He213 Weir, Greg A. aut Schiavo, Giampietro aut Enthalten in BMC Research Notes London, 2008 9(2016), 1 vom: 11. Feb. (DE-627)559431805 (DE-600)2413336-X 1756-0500 nnns volume:9 year:2016 number:1 day:11 month:02 https://dx.doi.org/10.1186/s13104-016-1915-8 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2016 1 11 02
language	English
source	Enthalten in BMC Research Notes 9(2016), 1 vom: 11. Feb. volume:9 year:2016 number:1 day:11 month:02
sourceStr	Enthalten in BMC Research Notes 9(2016), 1 vom: 11. Feb. volume:9 year:2016 number:1 day:11 month:02
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Dorsal root ganglia (DRG) Sensory neuron Peripheral neuropathy Primary culture
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container_title	BMC Research Notes
authorswithroles_txt_mv	Sleigh, James N. @@aut@@ Weir, Greg A. @@aut@@ Schiavo, Giampietro @@aut@@
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Rodent DRG neurons have long been studied in the laboratory to improve understanding of sensory nerve development and function, and have been instrumental in determining mechanisms underlying pain and neurodegeneration in disorders of the peripheral nervous system. Here, we describe a simple, step-by-step protocol for the swift isolation of mouse DRG, which can be enzymatically dissociated to produce fully differentiated primary neuronal cultures, or processed for downstream analyses, such as immunohistochemistry or RNA profiling. Findings After dissecting out the spinal column, from the base of the skull to the level of the femurs, it can be cut down the mid-line and the spinal cord and meninges removed, before extracting the DRG and detaching unwanted axons. This protocol allows the easy and rapid isolation of DRG with minimal practice and dissection experience. The process is both faster and less technically challenging than extracting the ganglia from the in situ column after performing a dorsal laminectomy. 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author	Sleigh, James N.
spellingShingle	Sleigh, James N. misc Dorsal root ganglia (DRG) misc Sensory neuron misc Peripheral neuropathy misc Primary culture A simple, step-by-step dissection protocol for the rapid isolation of mouse dorsal root ganglia
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topic_title	A simple, step-by-step dissection protocol for the rapid isolation of mouse dorsal root ganglia Dorsal root ganglia (DRG) (dpeaa)DE-He213 Sensory neuron (dpeaa)DE-He213 Peripheral neuropathy (dpeaa)DE-He213 Primary culture (dpeaa)DE-He213
topic	misc Dorsal root ganglia (DRG) misc Sensory neuron misc Peripheral neuropathy misc Primary culture
topic_unstemmed	misc Dorsal root ganglia (DRG) misc Sensory neuron misc Peripheral neuropathy misc Primary culture
topic_browse	misc Dorsal root ganglia (DRG) misc Sensory neuron misc Peripheral neuropathy misc Primary culture
format_facet	Elektronische Aufsätze Aufsätze Elektronische Ressource
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title	A simple, step-by-step dissection protocol for the rapid isolation of mouse dorsal root ganglia
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title_full	A simple, step-by-step dissection protocol for the rapid isolation of mouse dorsal root ganglia
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author_browse	Sleigh, James N. Weir, Greg A. Schiavo, Giampietro
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format_se	Elektronische Aufsätze
author-letter	Sleigh, James N.
doi_str_mv	10.1186/s13104-016-1915-8
title_sort	simple, step-by-step dissection protocol for the rapid isolation of mouse dorsal root ganglia
title_auth	A simple, step-by-step dissection protocol for the rapid isolation of mouse dorsal root ganglia
abstract	Background The cell bodies of sensory neurons, which transmit information from the external environment to the spinal cord, can be found at all levels of the spinal column in paired structures called dorsal root ganglia (DRG). Rodent DRG neurons have long been studied in the laboratory to improve understanding of sensory nerve development and function, and have been instrumental in determining mechanisms underlying pain and neurodegeneration in disorders of the peripheral nervous system. Here, we describe a simple, step-by-step protocol for the swift isolation of mouse DRG, which can be enzymatically dissociated to produce fully differentiated primary neuronal cultures, or processed for downstream analyses, such as immunohistochemistry or RNA profiling. Findings After dissecting out the spinal column, from the base of the skull to the level of the femurs, it can be cut down the mid-line and the spinal cord and meninges removed, before extracting the DRG and detaching unwanted axons. This protocol allows the easy and rapid isolation of DRG with minimal practice and dissection experience. The process is both faster and less technically challenging than extracting the ganglia from the in situ column after performing a dorsal laminectomy. Conclusions This approach reduces the time required to collect DRG, thereby improving efficiency, permitting less opportunity for tissue deterioration, and, ultimately, increasing the chances of generating healthy primary DRG cultures or high quality, reproducible experiments using DRG tissue. © Sleigh et al. 2016
abstractGer	Background The cell bodies of sensory neurons, which transmit information from the external environment to the spinal cord, can be found at all levels of the spinal column in paired structures called dorsal root ganglia (DRG). Rodent DRG neurons have long been studied in the laboratory to improve understanding of sensory nerve development and function, and have been instrumental in determining mechanisms underlying pain and neurodegeneration in disorders of the peripheral nervous system. Here, we describe a simple, step-by-step protocol for the swift isolation of mouse DRG, which can be enzymatically dissociated to produce fully differentiated primary neuronal cultures, or processed for downstream analyses, such as immunohistochemistry or RNA profiling. Findings After dissecting out the spinal column, from the base of the skull to the level of the femurs, it can be cut down the mid-line and the spinal cord and meninges removed, before extracting the DRG and detaching unwanted axons. This protocol allows the easy and rapid isolation of DRG with minimal practice and dissection experience. The process is both faster and less technically challenging than extracting the ganglia from the in situ column after performing a dorsal laminectomy. Conclusions This approach reduces the time required to collect DRG, thereby improving efficiency, permitting less opportunity for tissue deterioration, and, ultimately, increasing the chances of generating healthy primary DRG cultures or high quality, reproducible experiments using DRG tissue. © Sleigh et al. 2016
abstract_unstemmed	Background The cell bodies of sensory neurons, which transmit information from the external environment to the spinal cord, can be found at all levels of the spinal column in paired structures called dorsal root ganglia (DRG). Rodent DRG neurons have long been studied in the laboratory to improve understanding of sensory nerve development and function, and have been instrumental in determining mechanisms underlying pain and neurodegeneration in disorders of the peripheral nervous system. Here, we describe a simple, step-by-step protocol for the swift isolation of mouse DRG, which can be enzymatically dissociated to produce fully differentiated primary neuronal cultures, or processed for downstream analyses, such as immunohistochemistry or RNA profiling. Findings After dissecting out the spinal column, from the base of the skull to the level of the femurs, it can be cut down the mid-line and the spinal cord and meninges removed, before extracting the DRG and detaching unwanted axons. This protocol allows the easy and rapid isolation of DRG with minimal practice and dissection experience. The process is both faster and less technically challenging than extracting the ganglia from the in situ column after performing a dorsal laminectomy. Conclusions This approach reduces the time required to collect DRG, thereby improving efficiency, permitting less opportunity for tissue deterioration, and, ultimately, increasing the chances of generating healthy primary DRG cultures or high quality, reproducible experiments using DRG tissue. © Sleigh et al. 2016
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title_short	A simple, step-by-step dissection protocol for the rapid isolation of mouse dorsal root ganglia
url	https://dx.doi.org/10.1186/s13104-016-1915-8
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author2	Weir, Greg A. Schiavo, Giampietro
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up_date	2024-07-03T15:22:59.579Z
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A simple, step-by-step dissection protocol for the rapid isolation of mouse dorsal root ganglia

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