Modulation of endoglin expression in islets of langerhans by VEGF reveals a novel regulator of islet endothelial cell function

Background Endoglin/CD105 is an auxiliary receptor for transforming growth factor-β with established roles in vascular remodelling. It has recently been shown that heterozygous endoglin deficiency in mice decreases insulin secretion in an animal model of obesity, highlighting a potential role for en...
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Autor*in:	Clarkin, Claire E. [verfasserIn] Mahmoud, Marwa Liu, Bo Sobamowo, Emmanuel O. King, Aileen Arthur, Helen Jones, Peter M. Wheeler-Jones, Caroline P.

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2016

Schlagwörter:	Islets Endoglin VEGF Hypoxia inducible factor-1 alpha

Anmerkung:	© The Author(s) 2016

Übergeordnetes Werk:	Enthalten in: BMC Research Notes - London, 2008, 9(2016), 1 vom: 25. Juli
Übergeordnetes Werk:	volume:9 ; year:2016 ; number:1 ; day:25 ; month:07

Links:	Volltext

DOI / URN:	10.1186/s13104-016-2142-z

Katalog-ID:	SPR030317258

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520			\|a Background Endoglin/CD105 is an auxiliary receptor for transforming growth factor-β with established roles in vascular remodelling. It has recently been shown that heterozygous endoglin deficiency in mice decreases insulin secretion in an animal model of obesity, highlighting a potential role for endoglin in the regulation of islet function. We have previously identified two different populations of endoglin expressing cells in human and mouse islets which are: (i) endothelial cells (ECs) and (ii) islet mesenchymal stromal cells. The contribution of islet EC endoglin expression to islet development and sensitivity to VEGF is unknown and is the focus of this study. Results In vitro culture of mouse islets with $ VEGF_{164} $ for 48 h increased endoglin mRNA levels above untreated controls but VEGF did not modulate VEGFR2, CD31 or CD34 mRNA expression or islet viability. Removal of EC-endoglin expression in vivo reduced islet EC area but had no apparent effect on islet size or architecture. Conclusion EC-specific endoglin expression in islets is sensitive to VEGF and plays partial roles in driving islet vascular development, however such regulation appears to be distinct to mechanisms required to modulate islet viability and size.
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700	1		\|a King, Aileen \|4 aut
700	1		\|a Arthur, Helen \|4 aut
700	1		\|a Jones, Peter M. \|4 aut
700	1		\|a Wheeler-Jones, Caroline P. \|4 aut
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Indexfelder

author_variant	c e c ce cec m m mm b l bl e o s eo eos a k ak h a ha p m j pm pmj c p w j cpw cpwj
matchkey_str	article:17560500:2016----::ouainfnolnxrsinnsesfagrasyefeelaoergltrf
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publishDate	2016
allfields	10.1186/s13104-016-2142-z doi (DE-627)SPR030317258 (SPR)s13104-016-2142-z-e DE-627 ger DE-627 rakwb eng Clarkin, Claire E. verfasserin (orcid)0000-0001-6859-5897 aut Modulation of endoglin expression in islets of langerhans by VEGF reveals a novel regulator of islet endothelial cell function 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2016 Background Endoglin/CD105 is an auxiliary receptor for transforming growth factor-β with established roles in vascular remodelling. It has recently been shown that heterozygous endoglin deficiency in mice decreases insulin secretion in an animal model of obesity, highlighting a potential role for endoglin in the regulation of islet function. We have previously identified two different populations of endoglin expressing cells in human and mouse islets which are: (i) endothelial cells (ECs) and (ii) islet mesenchymal stromal cells. The contribution of islet EC endoglin expression to islet development and sensitivity to VEGF is unknown and is the focus of this study. Results In vitro culture of mouse islets with $ VEGF_{164} $ for 48 h increased endoglin mRNA levels above untreated controls but VEGF did not modulate VEGFR2, CD31 or CD34 mRNA expression or islet viability. Removal of EC-endoglin expression in vivo reduced islet EC area but had no apparent effect on islet size or architecture. Conclusion EC-specific endoglin expression in islets is sensitive to VEGF and plays partial roles in driving islet vascular development, however such regulation appears to be distinct to mechanisms required to modulate islet viability and size. Islets (dpeaa)DE-He213 Endoglin (dpeaa)DE-He213 VEGF (dpeaa)DE-He213 Hypoxia inducible factor-1 alpha (dpeaa)DE-He213 Mahmoud, Marwa aut Liu, Bo aut Sobamowo, Emmanuel O. aut King, Aileen aut Arthur, Helen aut Jones, Peter M. aut Wheeler-Jones, Caroline P. aut Enthalten in BMC Research Notes London, 2008 9(2016), 1 vom: 25. Juli (DE-627)559431805 (DE-600)2413336-X 1756-0500 nnns volume:9 year:2016 number:1 day:25 month:07 https://dx.doi.org/10.1186/s13104-016-2142-z kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2016 1 25 07
spelling	10.1186/s13104-016-2142-z doi (DE-627)SPR030317258 (SPR)s13104-016-2142-z-e DE-627 ger DE-627 rakwb eng Clarkin, Claire E. verfasserin (orcid)0000-0001-6859-5897 aut Modulation of endoglin expression in islets of langerhans by VEGF reveals a novel regulator of islet endothelial cell function 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2016 Background Endoglin/CD105 is an auxiliary receptor for transforming growth factor-β with established roles in vascular remodelling. It has recently been shown that heterozygous endoglin deficiency in mice decreases insulin secretion in an animal model of obesity, highlighting a potential role for endoglin in the regulation of islet function. We have previously identified two different populations of endoglin expressing cells in human and mouse islets which are: (i) endothelial cells (ECs) and (ii) islet mesenchymal stromal cells. The contribution of islet EC endoglin expression to islet development and sensitivity to VEGF is unknown and is the focus of this study. Results In vitro culture of mouse islets with $ VEGF_{164} $ for 48 h increased endoglin mRNA levels above untreated controls but VEGF did not modulate VEGFR2, CD31 or CD34 mRNA expression or islet viability. Removal of EC-endoglin expression in vivo reduced islet EC area but had no apparent effect on islet size or architecture. Conclusion EC-specific endoglin expression in islets is sensitive to VEGF and plays partial roles in driving islet vascular development, however such regulation appears to be distinct to mechanisms required to modulate islet viability and size. Islets (dpeaa)DE-He213 Endoglin (dpeaa)DE-He213 VEGF (dpeaa)DE-He213 Hypoxia inducible factor-1 alpha (dpeaa)DE-He213 Mahmoud, Marwa aut Liu, Bo aut Sobamowo, Emmanuel O. aut King, Aileen aut Arthur, Helen aut Jones, Peter M. aut Wheeler-Jones, Caroline P. aut Enthalten in BMC Research Notes London, 2008 9(2016), 1 vom: 25. Juli (DE-627)559431805 (DE-600)2413336-X 1756-0500 nnns volume:9 year:2016 number:1 day:25 month:07 https://dx.doi.org/10.1186/s13104-016-2142-z kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2016 1 25 07
allfields_unstemmed	10.1186/s13104-016-2142-z doi (DE-627)SPR030317258 (SPR)s13104-016-2142-z-e DE-627 ger DE-627 rakwb eng Clarkin, Claire E. verfasserin (orcid)0000-0001-6859-5897 aut Modulation of endoglin expression in islets of langerhans by VEGF reveals a novel regulator of islet endothelial cell function 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2016 Background Endoglin/CD105 is an auxiliary receptor for transforming growth factor-β with established roles in vascular remodelling. It has recently been shown that heterozygous endoglin deficiency in mice decreases insulin secretion in an animal model of obesity, highlighting a potential role for endoglin in the regulation of islet function. We have previously identified two different populations of endoglin expressing cells in human and mouse islets which are: (i) endothelial cells (ECs) and (ii) islet mesenchymal stromal cells. The contribution of islet EC endoglin expression to islet development and sensitivity to VEGF is unknown and is the focus of this study. Results In vitro culture of mouse islets with $ VEGF_{164} $ for 48 h increased endoglin mRNA levels above untreated controls but VEGF did not modulate VEGFR2, CD31 or CD34 mRNA expression or islet viability. Removal of EC-endoglin expression in vivo reduced islet EC area but had no apparent effect on islet size or architecture. Conclusion EC-specific endoglin expression in islets is sensitive to VEGF and plays partial roles in driving islet vascular development, however such regulation appears to be distinct to mechanisms required to modulate islet viability and size. Islets (dpeaa)DE-He213 Endoglin (dpeaa)DE-He213 VEGF (dpeaa)DE-He213 Hypoxia inducible factor-1 alpha (dpeaa)DE-He213 Mahmoud, Marwa aut Liu, Bo aut Sobamowo, Emmanuel O. aut King, Aileen aut Arthur, Helen aut Jones, Peter M. aut Wheeler-Jones, Caroline P. aut Enthalten in BMC Research Notes London, 2008 9(2016), 1 vom: 25. Juli (DE-627)559431805 (DE-600)2413336-X 1756-0500 nnns volume:9 year:2016 number:1 day:25 month:07 https://dx.doi.org/10.1186/s13104-016-2142-z kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2016 1 25 07
allfieldsGer	10.1186/s13104-016-2142-z doi (DE-627)SPR030317258 (SPR)s13104-016-2142-z-e DE-627 ger DE-627 rakwb eng Clarkin, Claire E. verfasserin (orcid)0000-0001-6859-5897 aut Modulation of endoglin expression in islets of langerhans by VEGF reveals a novel regulator of islet endothelial cell function 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2016 Background Endoglin/CD105 is an auxiliary receptor for transforming growth factor-β with established roles in vascular remodelling. It has recently been shown that heterozygous endoglin deficiency in mice decreases insulin secretion in an animal model of obesity, highlighting a potential role for endoglin in the regulation of islet function. We have previously identified two different populations of endoglin expressing cells in human and mouse islets which are: (i) endothelial cells (ECs) and (ii) islet mesenchymal stromal cells. The contribution of islet EC endoglin expression to islet development and sensitivity to VEGF is unknown and is the focus of this study. Results In vitro culture of mouse islets with $ VEGF_{164} $ for 48 h increased endoglin mRNA levels above untreated controls but VEGF did not modulate VEGFR2, CD31 or CD34 mRNA expression or islet viability. Removal of EC-endoglin expression in vivo reduced islet EC area but had no apparent effect on islet size or architecture. Conclusion EC-specific endoglin expression in islets is sensitive to VEGF and plays partial roles in driving islet vascular development, however such regulation appears to be distinct to mechanisms required to modulate islet viability and size. Islets (dpeaa)DE-He213 Endoglin (dpeaa)DE-He213 VEGF (dpeaa)DE-He213 Hypoxia inducible factor-1 alpha (dpeaa)DE-He213 Mahmoud, Marwa aut Liu, Bo aut Sobamowo, Emmanuel O. aut King, Aileen aut Arthur, Helen aut Jones, Peter M. aut Wheeler-Jones, Caroline P. aut Enthalten in BMC Research Notes London, 2008 9(2016), 1 vom: 25. Juli (DE-627)559431805 (DE-600)2413336-X 1756-0500 nnns volume:9 year:2016 number:1 day:25 month:07 https://dx.doi.org/10.1186/s13104-016-2142-z kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2016 1 25 07
allfieldsSound	10.1186/s13104-016-2142-z doi (DE-627)SPR030317258 (SPR)s13104-016-2142-z-e DE-627 ger DE-627 rakwb eng Clarkin, Claire E. verfasserin (orcid)0000-0001-6859-5897 aut Modulation of endoglin expression in islets of langerhans by VEGF reveals a novel regulator of islet endothelial cell function 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2016 Background Endoglin/CD105 is an auxiliary receptor for transforming growth factor-β with established roles in vascular remodelling. It has recently been shown that heterozygous endoglin deficiency in mice decreases insulin secretion in an animal model of obesity, highlighting a potential role for endoglin in the regulation of islet function. We have previously identified two different populations of endoglin expressing cells in human and mouse islets which are: (i) endothelial cells (ECs) and (ii) islet mesenchymal stromal cells. The contribution of islet EC endoglin expression to islet development and sensitivity to VEGF is unknown and is the focus of this study. Results In vitro culture of mouse islets with $ VEGF_{164} $ for 48 h increased endoglin mRNA levels above untreated controls but VEGF did not modulate VEGFR2, CD31 or CD34 mRNA expression or islet viability. Removal of EC-endoglin expression in vivo reduced islet EC area but had no apparent effect on islet size or architecture. Conclusion EC-specific endoglin expression in islets is sensitive to VEGF and plays partial roles in driving islet vascular development, however such regulation appears to be distinct to mechanisms required to modulate islet viability and size. Islets (dpeaa)DE-He213 Endoglin (dpeaa)DE-He213 VEGF (dpeaa)DE-He213 Hypoxia inducible factor-1 alpha (dpeaa)DE-He213 Mahmoud, Marwa aut Liu, Bo aut Sobamowo, Emmanuel O. aut King, Aileen aut Arthur, Helen aut Jones, Peter M. aut Wheeler-Jones, Caroline P. aut Enthalten in BMC Research Notes London, 2008 9(2016), 1 vom: 25. Juli (DE-627)559431805 (DE-600)2413336-X 1756-0500 nnns volume:9 year:2016 number:1 day:25 month:07 https://dx.doi.org/10.1186/s13104-016-2142-z kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2016 1 25 07
language	English
source	Enthalten in BMC Research Notes 9(2016), 1 vom: 25. Juli volume:9 year:2016 number:1 day:25 month:07
sourceStr	Enthalten in BMC Research Notes 9(2016), 1 vom: 25. Juli volume:9 year:2016 number:1 day:25 month:07
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Islets Endoglin VEGF Hypoxia inducible factor-1 alpha
isfreeaccess_bool	true
container_title	BMC Research Notes
authorswithroles_txt_mv	Clarkin, Claire E. @@aut@@ Mahmoud, Marwa @@aut@@ Liu, Bo @@aut@@ Sobamowo, Emmanuel O. @@aut@@ King, Aileen @@aut@@ Arthur, Helen @@aut@@ Jones, Peter M. @@aut@@ Wheeler-Jones, Caroline P. @@aut@@
publishDateDaySort_date	2016-07-25T00:00:00Z
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author	Clarkin, Claire E.
spellingShingle	Clarkin, Claire E. misc Islets misc Endoglin misc VEGF misc Hypoxia inducible factor-1 alpha Modulation of endoglin expression in islets of langerhans by VEGF reveals a novel regulator of islet endothelial cell function
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topic_title	Modulation of endoglin expression in islets of langerhans by VEGF reveals a novel regulator of islet endothelial cell function Islets (dpeaa)DE-He213 Endoglin (dpeaa)DE-He213 VEGF (dpeaa)DE-He213 Hypoxia inducible factor-1 alpha (dpeaa)DE-He213
topic	misc Islets misc Endoglin misc VEGF misc Hypoxia inducible factor-1 alpha
topic_unstemmed	misc Islets misc Endoglin misc VEGF misc Hypoxia inducible factor-1 alpha
topic_browse	misc Islets misc Endoglin misc VEGF misc Hypoxia inducible factor-1 alpha
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title	Modulation of endoglin expression in islets of langerhans by VEGF reveals a novel regulator of islet endothelial cell function
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title_full	Modulation of endoglin expression in islets of langerhans by VEGF reveals a novel regulator of islet endothelial cell function
author_sort	Clarkin, Claire E.
journal	BMC Research Notes
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author_browse	Clarkin, Claire E. Mahmoud, Marwa Liu, Bo Sobamowo, Emmanuel O. King, Aileen Arthur, Helen Jones, Peter M. Wheeler-Jones, Caroline P.
container_volume	9
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author-letter	Clarkin, Claire E.
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title_sort	modulation of endoglin expression in islets of langerhans by vegf reveals a novel regulator of islet endothelial cell function
title_auth	Modulation of endoglin expression in islets of langerhans by VEGF reveals a novel regulator of islet endothelial cell function
abstract	Background Endoglin/CD105 is an auxiliary receptor for transforming growth factor-β with established roles in vascular remodelling. It has recently been shown that heterozygous endoglin deficiency in mice decreases insulin secretion in an animal model of obesity, highlighting a potential role for endoglin in the regulation of islet function. We have previously identified two different populations of endoglin expressing cells in human and mouse islets which are: (i) endothelial cells (ECs) and (ii) islet mesenchymal stromal cells. The contribution of islet EC endoglin expression to islet development and sensitivity to VEGF is unknown and is the focus of this study. Results In vitro culture of mouse islets with $ VEGF_{164} $ for 48 h increased endoglin mRNA levels above untreated controls but VEGF did not modulate VEGFR2, CD31 or CD34 mRNA expression or islet viability. Removal of EC-endoglin expression in vivo reduced islet EC area but had no apparent effect on islet size or architecture. Conclusion EC-specific endoglin expression in islets is sensitive to VEGF and plays partial roles in driving islet vascular development, however such regulation appears to be distinct to mechanisms required to modulate islet viability and size. © The Author(s) 2016
abstractGer	Background Endoglin/CD105 is an auxiliary receptor for transforming growth factor-β with established roles in vascular remodelling. It has recently been shown that heterozygous endoglin deficiency in mice decreases insulin secretion in an animal model of obesity, highlighting a potential role for endoglin in the regulation of islet function. We have previously identified two different populations of endoglin expressing cells in human and mouse islets which are: (i) endothelial cells (ECs) and (ii) islet mesenchymal stromal cells. The contribution of islet EC endoglin expression to islet development and sensitivity to VEGF is unknown and is the focus of this study. Results In vitro culture of mouse islets with $ VEGF_{164} $ for 48 h increased endoglin mRNA levels above untreated controls but VEGF did not modulate VEGFR2, CD31 or CD34 mRNA expression or islet viability. Removal of EC-endoglin expression in vivo reduced islet EC area but had no apparent effect on islet size or architecture. Conclusion EC-specific endoglin expression in islets is sensitive to VEGF and plays partial roles in driving islet vascular development, however such regulation appears to be distinct to mechanisms required to modulate islet viability and size. © The Author(s) 2016
abstract_unstemmed	Background Endoglin/CD105 is an auxiliary receptor for transforming growth factor-β with established roles in vascular remodelling. It has recently been shown that heterozygous endoglin deficiency in mice decreases insulin secretion in an animal model of obesity, highlighting a potential role for endoglin in the regulation of islet function. We have previously identified two different populations of endoglin expressing cells in human and mouse islets which are: (i) endothelial cells (ECs) and (ii) islet mesenchymal stromal cells. The contribution of islet EC endoglin expression to islet development and sensitivity to VEGF is unknown and is the focus of this study. Results In vitro culture of mouse islets with $ VEGF_{164} $ for 48 h increased endoglin mRNA levels above untreated controls but VEGF did not modulate VEGFR2, CD31 or CD34 mRNA expression or islet viability. Removal of EC-endoglin expression in vivo reduced islet EC area but had no apparent effect on islet size or architecture. Conclusion EC-specific endoglin expression in islets is sensitive to VEGF and plays partial roles in driving islet vascular development, however such regulation appears to be distinct to mechanisms required to modulate islet viability and size. © The Author(s) 2016
collection_details	GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700
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title_short	Modulation of endoglin expression in islets of langerhans by VEGF reveals a novel regulator of islet endothelial cell function
url	https://dx.doi.org/10.1186/s13104-016-2142-z
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author2	Mahmoud, Marwa Liu, Bo Sobamowo, Emmanuel O. King, Aileen Arthur, Helen Jones, Peter M. Wheeler-Jones, Caroline P.
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doi_str	10.1186/s13104-016-2142-z
up_date	2024-07-03T15:24:02.670Z
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Modulation of endoglin expression in islets of langerhans by VEGF reveals a novel regulator of islet endothelial cell function

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