Cryptic diversity in an Atlantic Forest malaria vector from the mountains of South-East Brazil

Background Anopheles (Kerteszia) cruzii is the primary vector of human and simian malarias in Brazilian regions covered by the Atlantic Rainforest. Previous studies found that An. cruzii presents high levels of behavioural, chromosomal and molecular polymorphisms, which led to the hypothesis that it may be a complex of cryptic species. Here, An. cruzii specimens were collected in five sites in South-East Brazil located at different altitudes on the inner and coastal slopes of two mountain ranges covered by Atlantic Rainforest, known as Serra do Mar and Serra da Mantiqueria. Partial sequences for two genes (Clock and cpr) were generated and compared with previously published sequences from Florianópolis (southern Brazil). Genetic diversity was analysed with estimates of population structure (FST) and haplotype phylogenetic trees in order to understand how many species of the complex may occur in this biome and how populations across the species distribution are related. Results The sequences from specimens collected at sites located on the lower coastal slopes of Serra do Mar (Guapimirim, Tinguá and Sana) clustered together in the phylogenetic analysis, while the major haplotypes from sites located on higher altitude and at the continental side of the same mountains (Bocaina) clustered with those from Serra da Mantiqueira (Itatiaia), an inner mountain range. These two An. cruzii lineages showed statistically significant genetic differentiation and fixed characters, and have high FST values typical of between species comparisons. Finally, in Bocaina, where the two lineages occur in sympatry, we found deviations from Hardy-Weinberg equilibrium due to a deficit of heterozygotes, indicating partial reproductive isolation. These results strongly suggest that at least two distinct lineages of An. cruzii (provisorily named “Group 1” and “Group 2”) occur in the mountains of South-East Brazil. Conclusions At least two genetically distinct An. cruzii lineages occur in the Atlantic Forest covered mountains of South-East Brazil. The co-occurrence of distinct lineages of An. cruzii (possibly incipient species) in those mountains is an interesting biological phenomenon and may have important implications for malaria prevalence, Plasmodium transmission dynamics and control. Ausführliche Beschreibung

Gespeichert in:

Autor*in:	de Rezende Dias, Guilherme [verfasserIn] Fujii, Thais Tenorio Soares Fogel, Bernardo Fernandes Lourenço-de-Oliveira, Ricardo Silva-do-Nascimento, Teresa Fernandes Pitaluga, André Nóbrega Carvalho-Pinto, Carlos José Carvalho, Antonio Bernardo Peixoto, Alexandre Afrânio Rona, Luísa Damazio Pitaluga

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2018

Schlagwörter:	( ) Population genetics Mosquitoes Cryptic species Speciation Malaria

Anmerkung:	© The Author(s). 2018

Übergeordnetes Werk:	Enthalten in: Parasites & vectors - London : BioMed Central, 2008, 11(2018), 1 vom: 15. Jan.
Übergeordnetes Werk:	volume:11 ; year:2018 ; number:1 ; day:15 ; month:01

Links:	Volltext

DOI / URN:	10.1186/s13071-018-2615-0

Katalog-ID:	SPR030328853

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520			\|a Background Anopheles (Kerteszia) cruzii is the primary vector of human and simian malarias in Brazilian regions covered by the Atlantic Rainforest. Previous studies found that An. cruzii presents high levels of behavioural, chromosomal and molecular polymorphisms, which led to the hypothesis that it may be a complex of cryptic species. Here, An. cruzii specimens were collected in five sites in South-East Brazil located at different altitudes on the inner and coastal slopes of two mountain ranges covered by Atlantic Rainforest, known as Serra do Mar and Serra da Mantiqueria. Partial sequences for two genes (Clock and cpr) were generated and compared with previously published sequences from Florianópolis (southern Brazil). Genetic diversity was analysed with estimates of population structure (FST) and haplotype phylogenetic trees in order to understand how many species of the complex may occur in this biome and how populations across the species distribution are related. Results The sequences from specimens collected at sites located on the lower coastal slopes of Serra do Mar (Guapimirim, Tinguá and Sana) clustered together in the phylogenetic analysis, while the major haplotypes from sites located on higher altitude and at the continental side of the same mountains (Bocaina) clustered with those from Serra da Mantiqueira (Itatiaia), an inner mountain range. These two An. cruzii lineages showed statistically significant genetic differentiation and fixed characters, and have high FST values typical of between species comparisons. Finally, in Bocaina, where the two lineages occur in sympatry, we found deviations from Hardy-Weinberg equilibrium due to a deficit of heterozygotes, indicating partial reproductive isolation. These results strongly suggest that at least two distinct lineages of An. cruzii (provisorily named “Group 1” and “Group 2”) occur in the mountains of South-East Brazil. Conclusions At least two genetically distinct An. cruzii lineages occur in the Atlantic Forest covered mountains of South-East Brazil. The co-occurrence of distinct lineages of An. cruzii (possibly incipient species) in those mountains is an interesting biological phenomenon and may have important implications for malaria prevalence, Plasmodium transmission dynamics and control.
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allfields	10.1186/s13071-018-2615-0 doi (DE-627)SPR030328853 (SPR)s13071-018-2615-0-e DE-627 ger DE-627 rakwb eng de Rezende Dias, Guilherme verfasserin aut Cryptic diversity in an Atlantic Forest malaria vector from the mountains of South-East Brazil 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2018 Background Anopheles (Kerteszia) cruzii is the primary vector of human and simian malarias in Brazilian regions covered by the Atlantic Rainforest. Previous studies found that An. cruzii presents high levels of behavioural, chromosomal and molecular polymorphisms, which led to the hypothesis that it may be a complex of cryptic species. Here, An. cruzii specimens were collected in five sites in South-East Brazil located at different altitudes on the inner and coastal slopes of two mountain ranges covered by Atlantic Rainforest, known as Serra do Mar and Serra da Mantiqueria. Partial sequences for two genes (Clock and cpr) were generated and compared with previously published sequences from Florianópolis (southern Brazil). Genetic diversity was analysed with estimates of population structure (FST) and haplotype phylogenetic trees in order to understand how many species of the complex may occur in this biome and how populations across the species distribution are related. Results The sequences from specimens collected at sites located on the lower coastal slopes of Serra do Mar (Guapimirim, Tinguá and Sana) clustered together in the phylogenetic analysis, while the major haplotypes from sites located on higher altitude and at the continental side of the same mountains (Bocaina) clustered with those from Serra da Mantiqueira (Itatiaia), an inner mountain range. These two An. cruzii lineages showed statistically significant genetic differentiation and fixed characters, and have high FST values typical of between species comparisons. Finally, in Bocaina, where the two lineages occur in sympatry, we found deviations from Hardy-Weinberg equilibrium due to a deficit of heterozygotes, indicating partial reproductive isolation. These results strongly suggest that at least two distinct lineages of An. cruzii (provisorily named “Group 1” and “Group 2”) occur in the mountains of South-East Brazil. Conclusions At least two genetically distinct An. cruzii lineages occur in the Atlantic Forest covered mountains of South-East Brazil. The co-occurrence of distinct lineages of An. cruzii (possibly incipient species) in those mountains is an interesting biological phenomenon and may have important implications for malaria prevalence, Plasmodium transmission dynamics and control. ( (dpeaa)DE-He213 ) (dpeaa)DE-He213 Population genetics (dpeaa)DE-He213 Mosquitoes (dpeaa)DE-He213 Cryptic species (dpeaa)DE-He213 Speciation (dpeaa)DE-He213 Malaria (dpeaa)DE-He213 Fujii, Thais Tenorio Soares aut Fogel, Bernardo Fernandes aut Lourenço-de-Oliveira, Ricardo aut Silva-do-Nascimento, Teresa Fernandes aut Pitaluga, André Nóbrega aut Carvalho-Pinto, Carlos José aut Carvalho, Antonio Bernardo aut Peixoto, Alexandre Afrânio aut Rona, Luísa Damazio Pitaluga aut Enthalten in Parasites & vectors London : BioMed Central, 2008 11(2018), 1 vom: 15. Jan. (DE-627)558690076 (DE-600)2409480-8 1756-3305 nnns volume:11 year:2018 number:1 day:15 month:01 https://dx.doi.org/10.1186/s13071-018-2615-0 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2018 1 15 01
spelling	10.1186/s13071-018-2615-0 doi (DE-627)SPR030328853 (SPR)s13071-018-2615-0-e DE-627 ger DE-627 rakwb eng de Rezende Dias, Guilherme verfasserin aut Cryptic diversity in an Atlantic Forest malaria vector from the mountains of South-East Brazil 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2018 Background Anopheles (Kerteszia) cruzii is the primary vector of human and simian malarias in Brazilian regions covered by the Atlantic Rainforest. Previous studies found that An. cruzii presents high levels of behavioural, chromosomal and molecular polymorphisms, which led to the hypothesis that it may be a complex of cryptic species. Here, An. cruzii specimens were collected in five sites in South-East Brazil located at different altitudes on the inner and coastal slopes of two mountain ranges covered by Atlantic Rainforest, known as Serra do Mar and Serra da Mantiqueria. Partial sequences for two genes (Clock and cpr) were generated and compared with previously published sequences from Florianópolis (southern Brazil). Genetic diversity was analysed with estimates of population structure (FST) and haplotype phylogenetic trees in order to understand how many species of the complex may occur in this biome and how populations across the species distribution are related. Results The sequences from specimens collected at sites located on the lower coastal slopes of Serra do Mar (Guapimirim, Tinguá and Sana) clustered together in the phylogenetic analysis, while the major haplotypes from sites located on higher altitude and at the continental side of the same mountains (Bocaina) clustered with those from Serra da Mantiqueira (Itatiaia), an inner mountain range. These two An. cruzii lineages showed statistically significant genetic differentiation and fixed characters, and have high FST values typical of between species comparisons. Finally, in Bocaina, where the two lineages occur in sympatry, we found deviations from Hardy-Weinberg equilibrium due to a deficit of heterozygotes, indicating partial reproductive isolation. These results strongly suggest that at least two distinct lineages of An. cruzii (provisorily named “Group 1” and “Group 2”) occur in the mountains of South-East Brazil. Conclusions At least two genetically distinct An. cruzii lineages occur in the Atlantic Forest covered mountains of South-East Brazil. The co-occurrence of distinct lineages of An. cruzii (possibly incipient species) in those mountains is an interesting biological phenomenon and may have important implications for malaria prevalence, Plasmodium transmission dynamics and control. ( (dpeaa)DE-He213 ) (dpeaa)DE-He213 Population genetics (dpeaa)DE-He213 Mosquitoes (dpeaa)DE-He213 Cryptic species (dpeaa)DE-He213 Speciation (dpeaa)DE-He213 Malaria (dpeaa)DE-He213 Fujii, Thais Tenorio Soares aut Fogel, Bernardo Fernandes aut Lourenço-de-Oliveira, Ricardo aut Silva-do-Nascimento, Teresa Fernandes aut Pitaluga, André Nóbrega aut Carvalho-Pinto, Carlos José aut Carvalho, Antonio Bernardo aut Peixoto, Alexandre Afrânio aut Rona, Luísa Damazio Pitaluga aut Enthalten in Parasites & vectors London : BioMed Central, 2008 11(2018), 1 vom: 15. Jan. (DE-627)558690076 (DE-600)2409480-8 1756-3305 nnns volume:11 year:2018 number:1 day:15 month:01 https://dx.doi.org/10.1186/s13071-018-2615-0 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2018 1 15 01
allfields_unstemmed	10.1186/s13071-018-2615-0 doi (DE-627)SPR030328853 (SPR)s13071-018-2615-0-e DE-627 ger DE-627 rakwb eng de Rezende Dias, Guilherme verfasserin aut Cryptic diversity in an Atlantic Forest malaria vector from the mountains of South-East Brazil 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2018 Background Anopheles (Kerteszia) cruzii is the primary vector of human and simian malarias in Brazilian regions covered by the Atlantic Rainforest. Previous studies found that An. cruzii presents high levels of behavioural, chromosomal and molecular polymorphisms, which led to the hypothesis that it may be a complex of cryptic species. Here, An. cruzii specimens were collected in five sites in South-East Brazil located at different altitudes on the inner and coastal slopes of two mountain ranges covered by Atlantic Rainforest, known as Serra do Mar and Serra da Mantiqueria. Partial sequences for two genes (Clock and cpr) were generated and compared with previously published sequences from Florianópolis (southern Brazil). Genetic diversity was analysed with estimates of population structure (FST) and haplotype phylogenetic trees in order to understand how many species of the complex may occur in this biome and how populations across the species distribution are related. Results The sequences from specimens collected at sites located on the lower coastal slopes of Serra do Mar (Guapimirim, Tinguá and Sana) clustered together in the phylogenetic analysis, while the major haplotypes from sites located on higher altitude and at the continental side of the same mountains (Bocaina) clustered with those from Serra da Mantiqueira (Itatiaia), an inner mountain range. These two An. cruzii lineages showed statistically significant genetic differentiation and fixed characters, and have high FST values typical of between species comparisons. Finally, in Bocaina, where the two lineages occur in sympatry, we found deviations from Hardy-Weinberg equilibrium due to a deficit of heterozygotes, indicating partial reproductive isolation. These results strongly suggest that at least two distinct lineages of An. cruzii (provisorily named “Group 1” and “Group 2”) occur in the mountains of South-East Brazil. Conclusions At least two genetically distinct An. cruzii lineages occur in the Atlantic Forest covered mountains of South-East Brazil. The co-occurrence of distinct lineages of An. cruzii (possibly incipient species) in those mountains is an interesting biological phenomenon and may have important implications for malaria prevalence, Plasmodium transmission dynamics and control. ( (dpeaa)DE-He213 ) (dpeaa)DE-He213 Population genetics (dpeaa)DE-He213 Mosquitoes (dpeaa)DE-He213 Cryptic species (dpeaa)DE-He213 Speciation (dpeaa)DE-He213 Malaria (dpeaa)DE-He213 Fujii, Thais Tenorio Soares aut Fogel, Bernardo Fernandes aut Lourenço-de-Oliveira, Ricardo aut Silva-do-Nascimento, Teresa Fernandes aut Pitaluga, André Nóbrega aut Carvalho-Pinto, Carlos José aut Carvalho, Antonio Bernardo aut Peixoto, Alexandre Afrânio aut Rona, Luísa Damazio Pitaluga aut Enthalten in Parasites & vectors London : BioMed Central, 2008 11(2018), 1 vom: 15. Jan. (DE-627)558690076 (DE-600)2409480-8 1756-3305 nnns volume:11 year:2018 number:1 day:15 month:01 https://dx.doi.org/10.1186/s13071-018-2615-0 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2018 1 15 01
allfieldsGer	10.1186/s13071-018-2615-0 doi (DE-627)SPR030328853 (SPR)s13071-018-2615-0-e DE-627 ger DE-627 rakwb eng de Rezende Dias, Guilherme verfasserin aut Cryptic diversity in an Atlantic Forest malaria vector from the mountains of South-East Brazil 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2018 Background Anopheles (Kerteszia) cruzii is the primary vector of human and simian malarias in Brazilian regions covered by the Atlantic Rainforest. Previous studies found that An. cruzii presents high levels of behavioural, chromosomal and molecular polymorphisms, which led to the hypothesis that it may be a complex of cryptic species. Here, An. cruzii specimens were collected in five sites in South-East Brazil located at different altitudes on the inner and coastal slopes of two mountain ranges covered by Atlantic Rainforest, known as Serra do Mar and Serra da Mantiqueria. Partial sequences for two genes (Clock and cpr) were generated and compared with previously published sequences from Florianópolis (southern Brazil). Genetic diversity was analysed with estimates of population structure (FST) and haplotype phylogenetic trees in order to understand how many species of the complex may occur in this biome and how populations across the species distribution are related. Results The sequences from specimens collected at sites located on the lower coastal slopes of Serra do Mar (Guapimirim, Tinguá and Sana) clustered together in the phylogenetic analysis, while the major haplotypes from sites located on higher altitude and at the continental side of the same mountains (Bocaina) clustered with those from Serra da Mantiqueira (Itatiaia), an inner mountain range. These two An. cruzii lineages showed statistically significant genetic differentiation and fixed characters, and have high FST values typical of between species comparisons. Finally, in Bocaina, where the two lineages occur in sympatry, we found deviations from Hardy-Weinberg equilibrium due to a deficit of heterozygotes, indicating partial reproductive isolation. These results strongly suggest that at least two distinct lineages of An. cruzii (provisorily named “Group 1” and “Group 2”) occur in the mountains of South-East Brazil. Conclusions At least two genetically distinct An. cruzii lineages occur in the Atlantic Forest covered mountains of South-East Brazil. The co-occurrence of distinct lineages of An. cruzii (possibly incipient species) in those mountains is an interesting biological phenomenon and may have important implications for malaria prevalence, Plasmodium transmission dynamics and control. ( (dpeaa)DE-He213 ) (dpeaa)DE-He213 Population genetics (dpeaa)DE-He213 Mosquitoes (dpeaa)DE-He213 Cryptic species (dpeaa)DE-He213 Speciation (dpeaa)DE-He213 Malaria (dpeaa)DE-He213 Fujii, Thais Tenorio Soares aut Fogel, Bernardo Fernandes aut Lourenço-de-Oliveira, Ricardo aut Silva-do-Nascimento, Teresa Fernandes aut Pitaluga, André Nóbrega aut Carvalho-Pinto, Carlos José aut Carvalho, Antonio Bernardo aut Peixoto, Alexandre Afrânio aut Rona, Luísa Damazio Pitaluga aut Enthalten in Parasites & vectors London : BioMed Central, 2008 11(2018), 1 vom: 15. Jan. (DE-627)558690076 (DE-600)2409480-8 1756-3305 nnns volume:11 year:2018 number:1 day:15 month:01 https://dx.doi.org/10.1186/s13071-018-2615-0 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2018 1 15 01
allfieldsSound	10.1186/s13071-018-2615-0 doi (DE-627)SPR030328853 (SPR)s13071-018-2615-0-e DE-627 ger DE-627 rakwb eng de Rezende Dias, Guilherme verfasserin aut Cryptic diversity in an Atlantic Forest malaria vector from the mountains of South-East Brazil 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2018 Background Anopheles (Kerteszia) cruzii is the primary vector of human and simian malarias in Brazilian regions covered by the Atlantic Rainforest. Previous studies found that An. cruzii presents high levels of behavioural, chromosomal and molecular polymorphisms, which led to the hypothesis that it may be a complex of cryptic species. Here, An. cruzii specimens were collected in five sites in South-East Brazil located at different altitudes on the inner and coastal slopes of two mountain ranges covered by Atlantic Rainforest, known as Serra do Mar and Serra da Mantiqueria. Partial sequences for two genes (Clock and cpr) were generated and compared with previously published sequences from Florianópolis (southern Brazil). Genetic diversity was analysed with estimates of population structure (FST) and haplotype phylogenetic trees in order to understand how many species of the complex may occur in this biome and how populations across the species distribution are related. Results The sequences from specimens collected at sites located on the lower coastal slopes of Serra do Mar (Guapimirim, Tinguá and Sana) clustered together in the phylogenetic analysis, while the major haplotypes from sites located on higher altitude and at the continental side of the same mountains (Bocaina) clustered with those from Serra da Mantiqueira (Itatiaia), an inner mountain range. These two An. cruzii lineages showed statistically significant genetic differentiation and fixed characters, and have high FST values typical of between species comparisons. Finally, in Bocaina, where the two lineages occur in sympatry, we found deviations from Hardy-Weinberg equilibrium due to a deficit of heterozygotes, indicating partial reproductive isolation. These results strongly suggest that at least two distinct lineages of An. cruzii (provisorily named “Group 1” and “Group 2”) occur in the mountains of South-East Brazil. Conclusions At least two genetically distinct An. cruzii lineages occur in the Atlantic Forest covered mountains of South-East Brazil. The co-occurrence of distinct lineages of An. cruzii (possibly incipient species) in those mountains is an interesting biological phenomenon and may have important implications for malaria prevalence, Plasmodium transmission dynamics and control. ( (dpeaa)DE-He213 ) (dpeaa)DE-He213 Population genetics (dpeaa)DE-He213 Mosquitoes (dpeaa)DE-He213 Cryptic species (dpeaa)DE-He213 Speciation (dpeaa)DE-He213 Malaria (dpeaa)DE-He213 Fujii, Thais Tenorio Soares aut Fogel, Bernardo Fernandes aut Lourenço-de-Oliveira, Ricardo aut Silva-do-Nascimento, Teresa Fernandes aut Pitaluga, André Nóbrega aut Carvalho-Pinto, Carlos José aut Carvalho, Antonio Bernardo aut Peixoto, Alexandre Afrânio aut Rona, Luísa Damazio Pitaluga aut Enthalten in Parasites & vectors London : BioMed Central, 2008 11(2018), 1 vom: 15. Jan. (DE-627)558690076 (DE-600)2409480-8 1756-3305 nnns volume:11 year:2018 number:1 day:15 month:01 https://dx.doi.org/10.1186/s13071-018-2615-0 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2018 1 15 01
language	English
source	Enthalten in Parasites & vectors 11(2018), 1 vom: 15. Jan. volume:11 year:2018 number:1 day:15 month:01
sourceStr	Enthalten in Parasites & vectors 11(2018), 1 vom: 15. Jan. volume:11 year:2018 number:1 day:15 month:01
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	( ) Population genetics Mosquitoes Cryptic species Speciation Malaria
isfreeaccess_bool	true
container_title	Parasites & vectors
authorswithroles_txt_mv	de Rezende Dias, Guilherme @@aut@@ Fujii, Thais Tenorio Soares @@aut@@ Fogel, Bernardo Fernandes @@aut@@ Lourenço-de-Oliveira, Ricardo @@aut@@ Silva-do-Nascimento, Teresa Fernandes @@aut@@ Pitaluga, André Nóbrega @@aut@@ Carvalho-Pinto, Carlos José @@aut@@ Carvalho, Antonio Bernardo @@aut@@ Peixoto, Alexandre Afrânio @@aut@@ Rona, Luísa Damazio Pitaluga @@aut@@
publishDateDaySort_date	2018-01-15T00:00:00Z
hierarchy_top_id	558690076
id	SPR030328853
language_de	englisch
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Previous studies found that An. cruzii presents high levels of behavioural, chromosomal and molecular polymorphisms, which led to the hypothesis that it may be a complex of cryptic species. Here, An. cruzii specimens were collected in five sites in South-East Brazil located at different altitudes on the inner and coastal slopes of two mountain ranges covered by Atlantic Rainforest, known as Serra do Mar and Serra da Mantiqueria. Partial sequences for two genes (Clock and cpr) were generated and compared with previously published sequences from Florianópolis (southern Brazil). Genetic diversity was analysed with estimates of population structure (FST) and haplotype phylogenetic trees in order to understand how many species of the complex may occur in this biome and how populations across the species distribution are related. Results The sequences from specimens collected at sites located on the lower coastal slopes of Serra do Mar (Guapimirim, Tinguá and Sana) clustered together in the phylogenetic analysis, while the major haplotypes from sites located on higher altitude and at the continental side of the same mountains (Bocaina) clustered with those from Serra da Mantiqueira (Itatiaia), an inner mountain range. These two An. cruzii lineages showed statistically significant genetic differentiation and fixed characters, and have high FST values typical of between species comparisons. Finally, in Bocaina, where the two lineages occur in sympatry, we found deviations from Hardy-Weinberg equilibrium due to a deficit of heterozygotes, indicating partial reproductive isolation. These results strongly suggest that at least two distinct lineages of An. cruzii (provisorily named “Group 1” and “Group 2”) occur in the mountains of South-East Brazil. Conclusions At least two genetically distinct An. cruzii lineages occur in the Atlantic Forest covered mountains of South-East Brazil. 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author	de Rezende Dias, Guilherme
spellingShingle	de Rezende Dias, Guilherme misc ( misc ) misc Population genetics misc Mosquitoes misc Cryptic species misc Speciation misc Malaria Cryptic diversity in an Atlantic Forest malaria vector from the mountains of South-East Brazil
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topic_title	Cryptic diversity in an Atlantic Forest malaria vector from the mountains of South-East Brazil ( (dpeaa)DE-He213 ) (dpeaa)DE-He213 Population genetics (dpeaa)DE-He213 Mosquitoes (dpeaa)DE-He213 Cryptic species (dpeaa)DE-He213 Speciation (dpeaa)DE-He213 Malaria (dpeaa)DE-He213
topic	misc ( misc ) misc Population genetics misc Mosquitoes misc Cryptic species misc Speciation misc Malaria
topic_unstemmed	misc ( misc ) misc Population genetics misc Mosquitoes misc Cryptic species misc Speciation misc Malaria
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title	Cryptic diversity in an Atlantic Forest malaria vector from the mountains of South-East Brazil
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title_full	Cryptic diversity in an Atlantic Forest malaria vector from the mountains of South-East Brazil
author_sort	de Rezende Dias, Guilherme
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author_browse	de Rezende Dias, Guilherme Fujii, Thais Tenorio Soares Fogel, Bernardo Fernandes Lourenço-de-Oliveira, Ricardo Silva-do-Nascimento, Teresa Fernandes Pitaluga, André Nóbrega Carvalho-Pinto, Carlos José Carvalho, Antonio Bernardo Peixoto, Alexandre Afrânio Rona, Luísa Damazio Pitaluga
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format_se	Elektronische Aufsätze
author-letter	de Rezende Dias, Guilherme
doi_str_mv	10.1186/s13071-018-2615-0
title_sort	cryptic diversity in an atlantic forest malaria vector from the mountains of south-east brazil
title_auth	Cryptic diversity in an Atlantic Forest malaria vector from the mountains of South-East Brazil
abstract	Background Anopheles (Kerteszia) cruzii is the primary vector of human and simian malarias in Brazilian regions covered by the Atlantic Rainforest. Previous studies found that An. cruzii presents high levels of behavioural, chromosomal and molecular polymorphisms, which led to the hypothesis that it may be a complex of cryptic species. Here, An. cruzii specimens were collected in five sites in South-East Brazil located at different altitudes on the inner and coastal slopes of two mountain ranges covered by Atlantic Rainforest, known as Serra do Mar and Serra da Mantiqueria. Partial sequences for two genes (Clock and cpr) were generated and compared with previously published sequences from Florianópolis (southern Brazil). Genetic diversity was analysed with estimates of population structure (FST) and haplotype phylogenetic trees in order to understand how many species of the complex may occur in this biome and how populations across the species distribution are related. Results The sequences from specimens collected at sites located on the lower coastal slopes of Serra do Mar (Guapimirim, Tinguá and Sana) clustered together in the phylogenetic analysis, while the major haplotypes from sites located on higher altitude and at the continental side of the same mountains (Bocaina) clustered with those from Serra da Mantiqueira (Itatiaia), an inner mountain range. These two An. cruzii lineages showed statistically significant genetic differentiation and fixed characters, and have high FST values typical of between species comparisons. Finally, in Bocaina, where the two lineages occur in sympatry, we found deviations from Hardy-Weinberg equilibrium due to a deficit of heterozygotes, indicating partial reproductive isolation. These results strongly suggest that at least two distinct lineages of An. cruzii (provisorily named “Group 1” and “Group 2”) occur in the mountains of South-East Brazil. Conclusions At least two genetically distinct An. cruzii lineages occur in the Atlantic Forest covered mountains of South-East Brazil. The co-occurrence of distinct lineages of An. cruzii (possibly incipient species) in those mountains is an interesting biological phenomenon and may have important implications for malaria prevalence, Plasmodium transmission dynamics and control. © The Author(s). 2018
abstractGer	Background Anopheles (Kerteszia) cruzii is the primary vector of human and simian malarias in Brazilian regions covered by the Atlantic Rainforest. Previous studies found that An. cruzii presents high levels of behavioural, chromosomal and molecular polymorphisms, which led to the hypothesis that it may be a complex of cryptic species. Here, An. cruzii specimens were collected in five sites in South-East Brazil located at different altitudes on the inner and coastal slopes of two mountain ranges covered by Atlantic Rainforest, known as Serra do Mar and Serra da Mantiqueria. Partial sequences for two genes (Clock and cpr) were generated and compared with previously published sequences from Florianópolis (southern Brazil). Genetic diversity was analysed with estimates of population structure (FST) and haplotype phylogenetic trees in order to understand how many species of the complex may occur in this biome and how populations across the species distribution are related. Results The sequences from specimens collected at sites located on the lower coastal slopes of Serra do Mar (Guapimirim, Tinguá and Sana) clustered together in the phylogenetic analysis, while the major haplotypes from sites located on higher altitude and at the continental side of the same mountains (Bocaina) clustered with those from Serra da Mantiqueira (Itatiaia), an inner mountain range. These two An. cruzii lineages showed statistically significant genetic differentiation and fixed characters, and have high FST values typical of between species comparisons. Finally, in Bocaina, where the two lineages occur in sympatry, we found deviations from Hardy-Weinberg equilibrium due to a deficit of heterozygotes, indicating partial reproductive isolation. These results strongly suggest that at least two distinct lineages of An. cruzii (provisorily named “Group 1” and “Group 2”) occur in the mountains of South-East Brazil. Conclusions At least two genetically distinct An. cruzii lineages occur in the Atlantic Forest covered mountains of South-East Brazil. The co-occurrence of distinct lineages of An. cruzii (possibly incipient species) in those mountains is an interesting biological phenomenon and may have important implications for malaria prevalence, Plasmodium transmission dynamics and control. © The Author(s). 2018
abstract_unstemmed	Background Anopheles (Kerteszia) cruzii is the primary vector of human and simian malarias in Brazilian regions covered by the Atlantic Rainforest. Previous studies found that An. cruzii presents high levels of behavioural, chromosomal and molecular polymorphisms, which led to the hypothesis that it may be a complex of cryptic species. Here, An. cruzii specimens were collected in five sites in South-East Brazil located at different altitudes on the inner and coastal slopes of two mountain ranges covered by Atlantic Rainforest, known as Serra do Mar and Serra da Mantiqueria. Partial sequences for two genes (Clock and cpr) were generated and compared with previously published sequences from Florianópolis (southern Brazil). Genetic diversity was analysed with estimates of population structure (FST) and haplotype phylogenetic trees in order to understand how many species of the complex may occur in this biome and how populations across the species distribution are related. Results The sequences from specimens collected at sites located on the lower coastal slopes of Serra do Mar (Guapimirim, Tinguá and Sana) clustered together in the phylogenetic analysis, while the major haplotypes from sites located on higher altitude and at the continental side of the same mountains (Bocaina) clustered with those from Serra da Mantiqueira (Itatiaia), an inner mountain range. These two An. cruzii lineages showed statistically significant genetic differentiation and fixed characters, and have high FST values typical of between species comparisons. Finally, in Bocaina, where the two lineages occur in sympatry, we found deviations from Hardy-Weinberg equilibrium due to a deficit of heterozygotes, indicating partial reproductive isolation. These results strongly suggest that at least two distinct lineages of An. cruzii (provisorily named “Group 1” and “Group 2”) occur in the mountains of South-East Brazil. Conclusions At least two genetically distinct An. cruzii lineages occur in the Atlantic Forest covered mountains of South-East Brazil. The co-occurrence of distinct lineages of An. cruzii (possibly incipient species) in those mountains is an interesting biological phenomenon and may have important implications for malaria prevalence, Plasmodium transmission dynamics and control. © The Author(s). 2018
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title_short	Cryptic diversity in an Atlantic Forest malaria vector from the mountains of South-East Brazil
url	https://dx.doi.org/10.1186/s13071-018-2615-0
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author2	Fujii, Thais Tenorio Soares Fogel, Bernardo Fernandes Lourenço-de-Oliveira, Ricardo Silva-do-Nascimento, Teresa Fernandes Pitaluga, André Nóbrega Carvalho-Pinto, Carlos José Carvalho, Antonio Bernardo Peixoto, Alexandre Afrânio Rona, Luísa Damazio Pitaluga
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Cryptic diversity in an Atlantic Forest malaria vector from the mountains of South-East Brazil

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