Investigating the blood-host plasticity and dispersal of Anopheles coluzzii using a novel field-based methodology

Background The biting behaviour and dispersal of insect vectors in the field underlies the transmission of many diseases. Here, a novel collection methodology coupled with the molecular analysis of blood-meal sources and digestion rates is introduced with the aim of aiding the understanding of two critical and relatively understudied mosquito behaviours: plasticity in blood-host choice and vector dispersal. Results A collection strategy utilising a transect of mosquito traps placed at 50 m intervals allowed the collection of blood-fed Anopheles coluzzii from a malaria-endemic village of southern Ghana where human host availability ranged from zero (a cattle pen), increasing until humans were the dominant host choice (the middle of the village). Blood-meal analysis using PCR showed statistically significant variation in blood-meal origins for mosquitoes collected across the 250 m transect: with decreasing trend in Bovine Blood Index (OR = 0.60 95% CI: 0.49–0.73, P < 0.01) and correspondingly, an increasing trend in Human Blood Index (OR = 1.50 95% CI: 1.05–2.16, P = 0.028) as the transect approached the village. Using qPCR, the host DNA remaining in the blood meal was quantified for field-caught mosquitoes and calibrated according to timed blood digestion in colony mosquitoes. Time since blood meal was consumed and the corresponding distance the vector was caught from its blood-host allowed the estimation of An. coluzzii dispersal rates. Within 7 hours of feeding, mosquitoes typically remained within 50 m of their blood-host but at 60 hours they had dispersed up to 250 m. Conclusions Using this methodology the remarkably small spatial scale at which An. coluzzii blood-host choice can change was demonstrated. In addition, conducting qPCR on host blood from field-caught mosquitoes and calibrating with timed experiments with colonised mosquitoes presents a novel methodology for investigating the dispersal behaviour of vectors. Future adaptations to this novel method to make it broadly applicable to other types of setting are also discussed. Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Orsborne, James [verfasserIn] Furuya-Kanamori, Luis Jeffries, Claire L. Kristan, Mojca Mohammed, Abdul Rahim Afrane, Yaw A. O’Reilly, Kathleen Massad, Eduardo Drakeley, Chris Walker, Thomas Yakob, Laith

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2019

Schlagwörter:	Blood-meal analysis Host preference Mosquito Biting preference Blood index

Anmerkung:	© The Author(s) 2019

Übergeordnetes Werk:	Enthalten in: Parasites & vectors - London : BioMed Central, 2008, 12(2019), 1 vom: 25. März
Übergeordnetes Werk:	volume:12 ; year:2019 ; number:1 ; day:25 ; month:03

Links:	Volltext

DOI / URN:	10.1186/s13071-019-3401-3

Katalog-ID:	SPR030411874

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allfields	10.1186/s13071-019-3401-3 doi (DE-627)SPR030411874 (SPR)s13071-019-3401-3-e DE-627 ger DE-627 rakwb eng Orsborne, James verfasserin aut Investigating the blood-host plasticity and dispersal of Anopheles coluzzii using a novel field-based methodology 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2019 Background The biting behaviour and dispersal of insect vectors in the field underlies the transmission of many diseases. Here, a novel collection methodology coupled with the molecular analysis of blood-meal sources and digestion rates is introduced with the aim of aiding the understanding of two critical and relatively understudied mosquito behaviours: plasticity in blood-host choice and vector dispersal. Results A collection strategy utilising a transect of mosquito traps placed at 50 m intervals allowed the collection of blood-fed Anopheles coluzzii from a malaria-endemic village of southern Ghana where human host availability ranged from zero (a cattle pen), increasing until humans were the dominant host choice (the middle of the village). Blood-meal analysis using PCR showed statistically significant variation in blood-meal origins for mosquitoes collected across the 250 m transect: with decreasing trend in Bovine Blood Index (OR = 0.60 95% CI: 0.49–0.73, P < 0.01) and correspondingly, an increasing trend in Human Blood Index (OR = 1.50 95% CI: 1.05–2.16, P = 0.028) as the transect approached the village. Using qPCR, the host DNA remaining in the blood meal was quantified for field-caught mosquitoes and calibrated according to timed blood digestion in colony mosquitoes. Time since blood meal was consumed and the corresponding distance the vector was caught from its blood-host allowed the estimation of An. coluzzii dispersal rates. Within 7 hours of feeding, mosquitoes typically remained within 50 m of their blood-host but at 60 hours they had dispersed up to 250 m. Conclusions Using this methodology the remarkably small spatial scale at which An. coluzzii blood-host choice can change was demonstrated. In addition, conducting qPCR on host blood from field-caught mosquitoes and calibrating with timed experiments with colonised mosquitoes presents a novel methodology for investigating the dispersal behaviour of vectors. Future adaptations to this novel method to make it broadly applicable to other types of setting are also discussed. Blood-meal analysis (dpeaa)DE-He213 Host preference (dpeaa)DE-He213 Mosquito (dpeaa)DE-He213 Biting preference (dpeaa)DE-He213 Blood index (dpeaa)DE-He213 Furuya-Kanamori, Luis aut Jeffries, Claire L. aut Kristan, Mojca aut Mohammed, Abdul Rahim aut Afrane, Yaw A. aut O’Reilly, Kathleen aut Massad, Eduardo aut Drakeley, Chris aut Walker, Thomas aut Yakob, Laith (orcid)0000-0001-8639-4511 aut Enthalten in Parasites & vectors London : BioMed Central, 2008 12(2019), 1 vom: 25. März (DE-627)558690076 (DE-600)2409480-8 1756-3305 nnns volume:12 year:2019 number:1 day:25 month:03 https://dx.doi.org/10.1186/s13071-019-3401-3 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2019 1 25 03
spelling	10.1186/s13071-019-3401-3 doi (DE-627)SPR030411874 (SPR)s13071-019-3401-3-e DE-627 ger DE-627 rakwb eng Orsborne, James verfasserin aut Investigating the blood-host plasticity and dispersal of Anopheles coluzzii using a novel field-based methodology 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2019 Background The biting behaviour and dispersal of insect vectors in the field underlies the transmission of many diseases. Here, a novel collection methodology coupled with the molecular analysis of blood-meal sources and digestion rates is introduced with the aim of aiding the understanding of two critical and relatively understudied mosquito behaviours: plasticity in blood-host choice and vector dispersal. Results A collection strategy utilising a transect of mosquito traps placed at 50 m intervals allowed the collection of blood-fed Anopheles coluzzii from a malaria-endemic village of southern Ghana where human host availability ranged from zero (a cattle pen), increasing until humans were the dominant host choice (the middle of the village). Blood-meal analysis using PCR showed statistically significant variation in blood-meal origins for mosquitoes collected across the 250 m transect: with decreasing trend in Bovine Blood Index (OR = 0.60 95% CI: 0.49–0.73, P < 0.01) and correspondingly, an increasing trend in Human Blood Index (OR = 1.50 95% CI: 1.05–2.16, P = 0.028) as the transect approached the village. Using qPCR, the host DNA remaining in the blood meal was quantified for field-caught mosquitoes and calibrated according to timed blood digestion in colony mosquitoes. Time since blood meal was consumed and the corresponding distance the vector was caught from its blood-host allowed the estimation of An. coluzzii dispersal rates. Within 7 hours of feeding, mosquitoes typically remained within 50 m of their blood-host but at 60 hours they had dispersed up to 250 m. Conclusions Using this methodology the remarkably small spatial scale at which An. coluzzii blood-host choice can change was demonstrated. In addition, conducting qPCR on host blood from field-caught mosquitoes and calibrating with timed experiments with colonised mosquitoes presents a novel methodology for investigating the dispersal behaviour of vectors. Future adaptations to this novel method to make it broadly applicable to other types of setting are also discussed. Blood-meal analysis (dpeaa)DE-He213 Host preference (dpeaa)DE-He213 Mosquito (dpeaa)DE-He213 Biting preference (dpeaa)DE-He213 Blood index (dpeaa)DE-He213 Furuya-Kanamori, Luis aut Jeffries, Claire L. aut Kristan, Mojca aut Mohammed, Abdul Rahim aut Afrane, Yaw A. aut O’Reilly, Kathleen aut Massad, Eduardo aut Drakeley, Chris aut Walker, Thomas aut Yakob, Laith (orcid)0000-0001-8639-4511 aut Enthalten in Parasites & vectors London : BioMed Central, 2008 12(2019), 1 vom: 25. März (DE-627)558690076 (DE-600)2409480-8 1756-3305 nnns volume:12 year:2019 number:1 day:25 month:03 https://dx.doi.org/10.1186/s13071-019-3401-3 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2019 1 25 03
allfields_unstemmed	10.1186/s13071-019-3401-3 doi (DE-627)SPR030411874 (SPR)s13071-019-3401-3-e DE-627 ger DE-627 rakwb eng Orsborne, James verfasserin aut Investigating the blood-host plasticity and dispersal of Anopheles coluzzii using a novel field-based methodology 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2019 Background The biting behaviour and dispersal of insect vectors in the field underlies the transmission of many diseases. Here, a novel collection methodology coupled with the molecular analysis of blood-meal sources and digestion rates is introduced with the aim of aiding the understanding of two critical and relatively understudied mosquito behaviours: plasticity in blood-host choice and vector dispersal. Results A collection strategy utilising a transect of mosquito traps placed at 50 m intervals allowed the collection of blood-fed Anopheles coluzzii from a malaria-endemic village of southern Ghana where human host availability ranged from zero (a cattle pen), increasing until humans were the dominant host choice (the middle of the village). Blood-meal analysis using PCR showed statistically significant variation in blood-meal origins for mosquitoes collected across the 250 m transect: with decreasing trend in Bovine Blood Index (OR = 0.60 95% CI: 0.49–0.73, P < 0.01) and correspondingly, an increasing trend in Human Blood Index (OR = 1.50 95% CI: 1.05–2.16, P = 0.028) as the transect approached the village. Using qPCR, the host DNA remaining in the blood meal was quantified for field-caught mosquitoes and calibrated according to timed blood digestion in colony mosquitoes. Time since blood meal was consumed and the corresponding distance the vector was caught from its blood-host allowed the estimation of An. coluzzii dispersal rates. Within 7 hours of feeding, mosquitoes typically remained within 50 m of their blood-host but at 60 hours they had dispersed up to 250 m. Conclusions Using this methodology the remarkably small spatial scale at which An. coluzzii blood-host choice can change was demonstrated. In addition, conducting qPCR on host blood from field-caught mosquitoes and calibrating with timed experiments with colonised mosquitoes presents a novel methodology for investigating the dispersal behaviour of vectors. Future adaptations to this novel method to make it broadly applicable to other types of setting are also discussed. Blood-meal analysis (dpeaa)DE-He213 Host preference (dpeaa)DE-He213 Mosquito (dpeaa)DE-He213 Biting preference (dpeaa)DE-He213 Blood index (dpeaa)DE-He213 Furuya-Kanamori, Luis aut Jeffries, Claire L. aut Kristan, Mojca aut Mohammed, Abdul Rahim aut Afrane, Yaw A. aut O’Reilly, Kathleen aut Massad, Eduardo aut Drakeley, Chris aut Walker, Thomas aut Yakob, Laith (orcid)0000-0001-8639-4511 aut Enthalten in Parasites & vectors London : BioMed Central, 2008 12(2019), 1 vom: 25. März (DE-627)558690076 (DE-600)2409480-8 1756-3305 nnns volume:12 year:2019 number:1 day:25 month:03 https://dx.doi.org/10.1186/s13071-019-3401-3 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2019 1 25 03
allfieldsGer	10.1186/s13071-019-3401-3 doi (DE-627)SPR030411874 (SPR)s13071-019-3401-3-e DE-627 ger DE-627 rakwb eng Orsborne, James verfasserin aut Investigating the blood-host plasticity and dispersal of Anopheles coluzzii using a novel field-based methodology 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2019 Background The biting behaviour and dispersal of insect vectors in the field underlies the transmission of many diseases. Here, a novel collection methodology coupled with the molecular analysis of blood-meal sources and digestion rates is introduced with the aim of aiding the understanding of two critical and relatively understudied mosquito behaviours: plasticity in blood-host choice and vector dispersal. Results A collection strategy utilising a transect of mosquito traps placed at 50 m intervals allowed the collection of blood-fed Anopheles coluzzii from a malaria-endemic village of southern Ghana where human host availability ranged from zero (a cattle pen), increasing until humans were the dominant host choice (the middle of the village). Blood-meal analysis using PCR showed statistically significant variation in blood-meal origins for mosquitoes collected across the 250 m transect: with decreasing trend in Bovine Blood Index (OR = 0.60 95% CI: 0.49–0.73, P < 0.01) and correspondingly, an increasing trend in Human Blood Index (OR = 1.50 95% CI: 1.05–2.16, P = 0.028) as the transect approached the village. Using qPCR, the host DNA remaining in the blood meal was quantified for field-caught mosquitoes and calibrated according to timed blood digestion in colony mosquitoes. Time since blood meal was consumed and the corresponding distance the vector was caught from its blood-host allowed the estimation of An. coluzzii dispersal rates. Within 7 hours of feeding, mosquitoes typically remained within 50 m of their blood-host but at 60 hours they had dispersed up to 250 m. Conclusions Using this methodology the remarkably small spatial scale at which An. coluzzii blood-host choice can change was demonstrated. In addition, conducting qPCR on host blood from field-caught mosquitoes and calibrating with timed experiments with colonised mosquitoes presents a novel methodology for investigating the dispersal behaviour of vectors. Future adaptations to this novel method to make it broadly applicable to other types of setting are also discussed. Blood-meal analysis (dpeaa)DE-He213 Host preference (dpeaa)DE-He213 Mosquito (dpeaa)DE-He213 Biting preference (dpeaa)DE-He213 Blood index (dpeaa)DE-He213 Furuya-Kanamori, Luis aut Jeffries, Claire L. aut Kristan, Mojca aut Mohammed, Abdul Rahim aut Afrane, Yaw A. aut O’Reilly, Kathleen aut Massad, Eduardo aut Drakeley, Chris aut Walker, Thomas aut Yakob, Laith (orcid)0000-0001-8639-4511 aut Enthalten in Parasites & vectors London : BioMed Central, 2008 12(2019), 1 vom: 25. März (DE-627)558690076 (DE-600)2409480-8 1756-3305 nnns volume:12 year:2019 number:1 day:25 month:03 https://dx.doi.org/10.1186/s13071-019-3401-3 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2019 1 25 03
allfieldsSound	10.1186/s13071-019-3401-3 doi (DE-627)SPR030411874 (SPR)s13071-019-3401-3-e DE-627 ger DE-627 rakwb eng Orsborne, James verfasserin aut Investigating the blood-host plasticity and dispersal of Anopheles coluzzii using a novel field-based methodology 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2019 Background The biting behaviour and dispersal of insect vectors in the field underlies the transmission of many diseases. Here, a novel collection methodology coupled with the molecular analysis of blood-meal sources and digestion rates is introduced with the aim of aiding the understanding of two critical and relatively understudied mosquito behaviours: plasticity in blood-host choice and vector dispersal. Results A collection strategy utilising a transect of mosquito traps placed at 50 m intervals allowed the collection of blood-fed Anopheles coluzzii from a malaria-endemic village of southern Ghana where human host availability ranged from zero (a cattle pen), increasing until humans were the dominant host choice (the middle of the village). Blood-meal analysis using PCR showed statistically significant variation in blood-meal origins for mosquitoes collected across the 250 m transect: with decreasing trend in Bovine Blood Index (OR = 0.60 95% CI: 0.49–0.73, P < 0.01) and correspondingly, an increasing trend in Human Blood Index (OR = 1.50 95% CI: 1.05–2.16, P = 0.028) as the transect approached the village. Using qPCR, the host DNA remaining in the blood meal was quantified for field-caught mosquitoes and calibrated according to timed blood digestion in colony mosquitoes. Time since blood meal was consumed and the corresponding distance the vector was caught from its blood-host allowed the estimation of An. coluzzii dispersal rates. Within 7 hours of feeding, mosquitoes typically remained within 50 m of their blood-host but at 60 hours they had dispersed up to 250 m. Conclusions Using this methodology the remarkably small spatial scale at which An. coluzzii blood-host choice can change was demonstrated. In addition, conducting qPCR on host blood from field-caught mosquitoes and calibrating with timed experiments with colonised mosquitoes presents a novel methodology for investigating the dispersal behaviour of vectors. Future adaptations to this novel method to make it broadly applicable to other types of setting are also discussed. Blood-meal analysis (dpeaa)DE-He213 Host preference (dpeaa)DE-He213 Mosquito (dpeaa)DE-He213 Biting preference (dpeaa)DE-He213 Blood index (dpeaa)DE-He213 Furuya-Kanamori, Luis aut Jeffries, Claire L. aut Kristan, Mojca aut Mohammed, Abdul Rahim aut Afrane, Yaw A. aut O’Reilly, Kathleen aut Massad, Eduardo aut Drakeley, Chris aut Walker, Thomas aut Yakob, Laith (orcid)0000-0001-8639-4511 aut Enthalten in Parasites & vectors London : BioMed Central, 2008 12(2019), 1 vom: 25. März (DE-627)558690076 (DE-600)2409480-8 1756-3305 nnns volume:12 year:2019 number:1 day:25 month:03 https://dx.doi.org/10.1186/s13071-019-3401-3 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2019 1 25 03
language	English
source	Enthalten in Parasites & vectors 12(2019), 1 vom: 25. März volume:12 year:2019 number:1 day:25 month:03
sourceStr	Enthalten in Parasites & vectors 12(2019), 1 vom: 25. März volume:12 year:2019 number:1 day:25 month:03
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Blood-meal analysis Host preference Mosquito Biting preference Blood index
isfreeaccess_bool	true
container_title	Parasites & vectors
authorswithroles_txt_mv	Orsborne, James @@aut@@ Furuya-Kanamori, Luis @@aut@@ Jeffries, Claire L. @@aut@@ Kristan, Mojca @@aut@@ Mohammed, Abdul Rahim @@aut@@ Afrane, Yaw A. @@aut@@ O’Reilly, Kathleen @@aut@@ Massad, Eduardo @@aut@@ Drakeley, Chris @@aut@@ Walker, Thomas @@aut@@ Yakob, Laith @@aut@@
publishDateDaySort_date	2019-03-25T00:00:00Z
hierarchy_top_id	558690076
id	SPR030411874
language_de	englisch
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author	Orsborne, James
spellingShingle	Orsborne, James misc Blood-meal analysis misc Host preference misc Mosquito misc Biting preference misc Blood index Investigating the blood-host plasticity and dispersal of Anopheles coluzzii using a novel field-based methodology
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topic_title	Investigating the blood-host plasticity and dispersal of Anopheles coluzzii using a novel field-based methodology Blood-meal analysis (dpeaa)DE-He213 Host preference (dpeaa)DE-He213 Mosquito (dpeaa)DE-He213 Biting preference (dpeaa)DE-He213 Blood index (dpeaa)DE-He213
topic	misc Blood-meal analysis misc Host preference misc Mosquito misc Biting preference misc Blood index
topic_unstemmed	misc Blood-meal analysis misc Host preference misc Mosquito misc Biting preference misc Blood index
topic_browse	misc Blood-meal analysis misc Host preference misc Mosquito misc Biting preference misc Blood index
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title	Investigating the blood-host plasticity and dispersal of Anopheles coluzzii using a novel field-based methodology
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title_full	Investigating the blood-host plasticity and dispersal of Anopheles coluzzii using a novel field-based methodology
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author_browse	Orsborne, James Furuya-Kanamori, Luis Jeffries, Claire L. Kristan, Mojca Mohammed, Abdul Rahim Afrane, Yaw A. O’Reilly, Kathleen Massad, Eduardo Drakeley, Chris Walker, Thomas Yakob, Laith
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author-letter	Orsborne, James
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title_sort	investigating the blood-host plasticity and dispersal of anopheles coluzzii using a novel field-based methodology
title_auth	Investigating the blood-host plasticity and dispersal of Anopheles coluzzii using a novel field-based methodology
abstract	Background The biting behaviour and dispersal of insect vectors in the field underlies the transmission of many diseases. Here, a novel collection methodology coupled with the molecular analysis of blood-meal sources and digestion rates is introduced with the aim of aiding the understanding of two critical and relatively understudied mosquito behaviours: plasticity in blood-host choice and vector dispersal. Results A collection strategy utilising a transect of mosquito traps placed at 50 m intervals allowed the collection of blood-fed Anopheles coluzzii from a malaria-endemic village of southern Ghana where human host availability ranged from zero (a cattle pen), increasing until humans were the dominant host choice (the middle of the village). Blood-meal analysis using PCR showed statistically significant variation in blood-meal origins for mosquitoes collected across the 250 m transect: with decreasing trend in Bovine Blood Index (OR = 0.60 95% CI: 0.49–0.73, P < 0.01) and correspondingly, an increasing trend in Human Blood Index (OR = 1.50 95% CI: 1.05–2.16, P = 0.028) as the transect approached the village. Using qPCR, the host DNA remaining in the blood meal was quantified for field-caught mosquitoes and calibrated according to timed blood digestion in colony mosquitoes. Time since blood meal was consumed and the corresponding distance the vector was caught from its blood-host allowed the estimation of An. coluzzii dispersal rates. Within 7 hours of feeding, mosquitoes typically remained within 50 m of their blood-host but at 60 hours they had dispersed up to 250 m. Conclusions Using this methodology the remarkably small spatial scale at which An. coluzzii blood-host choice can change was demonstrated. In addition, conducting qPCR on host blood from field-caught mosquitoes and calibrating with timed experiments with colonised mosquitoes presents a novel methodology for investigating the dispersal behaviour of vectors. Future adaptations to this novel method to make it broadly applicable to other types of setting are also discussed. © The Author(s) 2019
abstractGer	Background The biting behaviour and dispersal of insect vectors in the field underlies the transmission of many diseases. Here, a novel collection methodology coupled with the molecular analysis of blood-meal sources and digestion rates is introduced with the aim of aiding the understanding of two critical and relatively understudied mosquito behaviours: plasticity in blood-host choice and vector dispersal. Results A collection strategy utilising a transect of mosquito traps placed at 50 m intervals allowed the collection of blood-fed Anopheles coluzzii from a malaria-endemic village of southern Ghana where human host availability ranged from zero (a cattle pen), increasing until humans were the dominant host choice (the middle of the village). Blood-meal analysis using PCR showed statistically significant variation in blood-meal origins for mosquitoes collected across the 250 m transect: with decreasing trend in Bovine Blood Index (OR = 0.60 95% CI: 0.49–0.73, P < 0.01) and correspondingly, an increasing trend in Human Blood Index (OR = 1.50 95% CI: 1.05–2.16, P = 0.028) as the transect approached the village. Using qPCR, the host DNA remaining in the blood meal was quantified for field-caught mosquitoes and calibrated according to timed blood digestion in colony mosquitoes. Time since blood meal was consumed and the corresponding distance the vector was caught from its blood-host allowed the estimation of An. coluzzii dispersal rates. Within 7 hours of feeding, mosquitoes typically remained within 50 m of their blood-host but at 60 hours they had dispersed up to 250 m. Conclusions Using this methodology the remarkably small spatial scale at which An. coluzzii blood-host choice can change was demonstrated. In addition, conducting qPCR on host blood from field-caught mosquitoes and calibrating with timed experiments with colonised mosquitoes presents a novel methodology for investigating the dispersal behaviour of vectors. Future adaptations to this novel method to make it broadly applicable to other types of setting are also discussed. © The Author(s) 2019
abstract_unstemmed	Background The biting behaviour and dispersal of insect vectors in the field underlies the transmission of many diseases. Here, a novel collection methodology coupled with the molecular analysis of blood-meal sources and digestion rates is introduced with the aim of aiding the understanding of two critical and relatively understudied mosquito behaviours: plasticity in blood-host choice and vector dispersal. Results A collection strategy utilising a transect of mosquito traps placed at 50 m intervals allowed the collection of blood-fed Anopheles coluzzii from a malaria-endemic village of southern Ghana where human host availability ranged from zero (a cattle pen), increasing until humans were the dominant host choice (the middle of the village). Blood-meal analysis using PCR showed statistically significant variation in blood-meal origins for mosquitoes collected across the 250 m transect: with decreasing trend in Bovine Blood Index (OR = 0.60 95% CI: 0.49–0.73, P < 0.01) and correspondingly, an increasing trend in Human Blood Index (OR = 1.50 95% CI: 1.05–2.16, P = 0.028) as the transect approached the village. Using qPCR, the host DNA remaining in the blood meal was quantified for field-caught mosquitoes and calibrated according to timed blood digestion in colony mosquitoes. Time since blood meal was consumed and the corresponding distance the vector was caught from its blood-host allowed the estimation of An. coluzzii dispersal rates. Within 7 hours of feeding, mosquitoes typically remained within 50 m of their blood-host but at 60 hours they had dispersed up to 250 m. Conclusions Using this methodology the remarkably small spatial scale at which An. coluzzii blood-host choice can change was demonstrated. In addition, conducting qPCR on host blood from field-caught mosquitoes and calibrating with timed experiments with colonised mosquitoes presents a novel methodology for investigating the dispersal behaviour of vectors. Future adaptations to this novel method to make it broadly applicable to other types of setting are also discussed. © The Author(s) 2019
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title_short	Investigating the blood-host plasticity and dispersal of Anopheles coluzzii using a novel field-based methodology
url	https://dx.doi.org/10.1186/s13071-019-3401-3
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author2	Furuya-Kanamori, Luis Jeffries, Claire L. Kristan, Mojca Mohammed, Abdul Rahim Afrane, Yaw A. O’Reilly, Kathleen Massad, Eduardo Drakeley, Chris Walker, Thomas Yakob, Laith
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