Definitive host influences the proteomic profile of excretory/secretory products of the trematode Echinostoma caproni

Background Echinostoma caproni is an intestinal trematode extensively used as experimental model for the study of factors that determine the course of intestinal helminth infections, since this markedly depends on the host species. Although the host-dependent mechanisms for either chronic establishm...
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Autor*in:	Cortés, Alba [verfasserIn] Sotillo, Javier Muñoz-Antolí, Carla Trelis, María Esteban, J. Guillermo Toledo, Rafael

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2016

Schlagwörter:	Helminth Excretory/secretory products Proteome plasticity 2-dimensional gel electrophoresis

Anmerkung:	© Cortés et al. 2016

Übergeordnetes Werk:	Enthalten in: Parasites & vectors - London : BioMed Central, 2008, 9(2016), 1 vom: 31. März
Übergeordnetes Werk:	volume:9 ; year:2016 ; number:1 ; day:31 ; month:03

Links:	Volltext

DOI / URN:	10.1186/s13071-016-1465-x

Katalog-ID:	SPR030841356

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520			\|a Background Echinostoma caproni is an intestinal trematode extensively used as experimental model for the study of factors that determine the course of intestinal helminth infections, since this markedly depends on the host species. Although the host-dependent mechanisms for either chronic establishment or early parasite rejection have been broadly studied, little is known regarding the parasite response against different host environments. Methods To identify host-dependent differentially expressed proteins, a comparative proteomic analysis of the excretory/secretory products released from E. caproni adults, isolated from hosts displaying different compatibility with this trematode, was performed. Results A total of 19 differential protein spots were identified (14 overexpressed in mice and 5 overexpressed in rats). The establishment of chronic infections in mice is mainly associated with the overexpression by adult worms of antioxidant and detoxifying enzymes (e.g. glutathione S-transferase, hydroxyacylglutathione hydrolase, thiopurine S-transferase, etc.) and metabolic enzymes like enolase, leucine aminopeptidase or malate dehydrogenase. However, the overexpression of cathepsin L and the structural protein actin observed in worms isolated from rats seems not to be effective for the colonization of the intestinal mucosa of this host. Conclusions The observed differences suggest that protein expression and/or release is modulated by the local environment generated inside the host and provide useful insights in regards to the resistance mechanisms developed by parasites to ensure their long-term survival.
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author_variant	a c ac j s js c m a cma m t mt j g e jg jge r t rt
matchkey_str	article:17563305:2016----::eiiieotnlecshpoemcrfloeceoyertrpoutot
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allfields	10.1186/s13071-016-1465-x doi (DE-627)SPR030841356 (SPR)s13071-016-1465-x-e DE-627 ger DE-627 rakwb eng Cortés, Alba verfasserin aut Definitive host influences the proteomic profile of excretory/secretory products of the trematode Echinostoma caproni 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Cortés et al. 2016 Background Echinostoma caproni is an intestinal trematode extensively used as experimental model for the study of factors that determine the course of intestinal helminth infections, since this markedly depends on the host species. Although the host-dependent mechanisms for either chronic establishment or early parasite rejection have been broadly studied, little is known regarding the parasite response against different host environments. Methods To identify host-dependent differentially expressed proteins, a comparative proteomic analysis of the excretory/secretory products released from E. caproni adults, isolated from hosts displaying different compatibility with this trematode, was performed. Results A total of 19 differential protein spots were identified (14 overexpressed in mice and 5 overexpressed in rats). The establishment of chronic infections in mice is mainly associated with the overexpression by adult worms of antioxidant and detoxifying enzymes (e.g. glutathione S-transferase, hydroxyacylglutathione hydrolase, thiopurine S-transferase, etc.) and metabolic enzymes like enolase, leucine aminopeptidase or malate dehydrogenase. However, the overexpression of cathepsin L and the structural protein actin observed in worms isolated from rats seems not to be effective for the colonization of the intestinal mucosa of this host. Conclusions The observed differences suggest that protein expression and/or release is modulated by the local environment generated inside the host and provide useful insights in regards to the resistance mechanisms developed by parasites to ensure their long-term survival. Helminth (dpeaa)DE-He213 Excretory/secretory products (dpeaa)DE-He213 Proteome plasticity (dpeaa)DE-He213 2-dimensional gel electrophoresis (dpeaa)DE-He213 Sotillo, Javier aut Muñoz-Antolí, Carla aut Trelis, María aut Esteban, J. Guillermo aut Toledo, Rafael aut Enthalten in Parasites & vectors London : BioMed Central, 2008 9(2016), 1 vom: 31. März (DE-627)558690076 (DE-600)2409480-8 1756-3305 nnns volume:9 year:2016 number:1 day:31 month:03 https://dx.doi.org/10.1186/s13071-016-1465-x kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2016 1 31 03
spelling	10.1186/s13071-016-1465-x doi (DE-627)SPR030841356 (SPR)s13071-016-1465-x-e DE-627 ger DE-627 rakwb eng Cortés, Alba verfasserin aut Definitive host influences the proteomic profile of excretory/secretory products of the trematode Echinostoma caproni 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Cortés et al. 2016 Background Echinostoma caproni is an intestinal trematode extensively used as experimental model for the study of factors that determine the course of intestinal helminth infections, since this markedly depends on the host species. Although the host-dependent mechanisms for either chronic establishment or early parasite rejection have been broadly studied, little is known regarding the parasite response against different host environments. Methods To identify host-dependent differentially expressed proteins, a comparative proteomic analysis of the excretory/secretory products released from E. caproni adults, isolated from hosts displaying different compatibility with this trematode, was performed. Results A total of 19 differential protein spots were identified (14 overexpressed in mice and 5 overexpressed in rats). The establishment of chronic infections in mice is mainly associated with the overexpression by adult worms of antioxidant and detoxifying enzymes (e.g. glutathione S-transferase, hydroxyacylglutathione hydrolase, thiopurine S-transferase, etc.) and metabolic enzymes like enolase, leucine aminopeptidase or malate dehydrogenase. However, the overexpression of cathepsin L and the structural protein actin observed in worms isolated from rats seems not to be effective for the colonization of the intestinal mucosa of this host. Conclusions The observed differences suggest that protein expression and/or release is modulated by the local environment generated inside the host and provide useful insights in regards to the resistance mechanisms developed by parasites to ensure their long-term survival. Helminth (dpeaa)DE-He213 Excretory/secretory products (dpeaa)DE-He213 Proteome plasticity (dpeaa)DE-He213 2-dimensional gel electrophoresis (dpeaa)DE-He213 Sotillo, Javier aut Muñoz-Antolí, Carla aut Trelis, María aut Esteban, J. Guillermo aut Toledo, Rafael aut Enthalten in Parasites & vectors London : BioMed Central, 2008 9(2016), 1 vom: 31. März (DE-627)558690076 (DE-600)2409480-8 1756-3305 nnns volume:9 year:2016 number:1 day:31 month:03 https://dx.doi.org/10.1186/s13071-016-1465-x kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2016 1 31 03
allfields_unstemmed	10.1186/s13071-016-1465-x doi (DE-627)SPR030841356 (SPR)s13071-016-1465-x-e DE-627 ger DE-627 rakwb eng Cortés, Alba verfasserin aut Definitive host influences the proteomic profile of excretory/secretory products of the trematode Echinostoma caproni 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Cortés et al. 2016 Background Echinostoma caproni is an intestinal trematode extensively used as experimental model for the study of factors that determine the course of intestinal helminth infections, since this markedly depends on the host species. Although the host-dependent mechanisms for either chronic establishment or early parasite rejection have been broadly studied, little is known regarding the parasite response against different host environments. Methods To identify host-dependent differentially expressed proteins, a comparative proteomic analysis of the excretory/secretory products released from E. caproni adults, isolated from hosts displaying different compatibility with this trematode, was performed. Results A total of 19 differential protein spots were identified (14 overexpressed in mice and 5 overexpressed in rats). The establishment of chronic infections in mice is mainly associated with the overexpression by adult worms of antioxidant and detoxifying enzymes (e.g. glutathione S-transferase, hydroxyacylglutathione hydrolase, thiopurine S-transferase, etc.) and metabolic enzymes like enolase, leucine aminopeptidase or malate dehydrogenase. However, the overexpression of cathepsin L and the structural protein actin observed in worms isolated from rats seems not to be effective for the colonization of the intestinal mucosa of this host. Conclusions The observed differences suggest that protein expression and/or release is modulated by the local environment generated inside the host and provide useful insights in regards to the resistance mechanisms developed by parasites to ensure their long-term survival. Helminth (dpeaa)DE-He213 Excretory/secretory products (dpeaa)DE-He213 Proteome plasticity (dpeaa)DE-He213 2-dimensional gel electrophoresis (dpeaa)DE-He213 Sotillo, Javier aut Muñoz-Antolí, Carla aut Trelis, María aut Esteban, J. Guillermo aut Toledo, Rafael aut Enthalten in Parasites & vectors London : BioMed Central, 2008 9(2016), 1 vom: 31. März (DE-627)558690076 (DE-600)2409480-8 1756-3305 nnns volume:9 year:2016 number:1 day:31 month:03 https://dx.doi.org/10.1186/s13071-016-1465-x kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2016 1 31 03
allfieldsGer	10.1186/s13071-016-1465-x doi (DE-627)SPR030841356 (SPR)s13071-016-1465-x-e DE-627 ger DE-627 rakwb eng Cortés, Alba verfasserin aut Definitive host influences the proteomic profile of excretory/secretory products of the trematode Echinostoma caproni 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Cortés et al. 2016 Background Echinostoma caproni is an intestinal trematode extensively used as experimental model for the study of factors that determine the course of intestinal helminth infections, since this markedly depends on the host species. Although the host-dependent mechanisms for either chronic establishment or early parasite rejection have been broadly studied, little is known regarding the parasite response against different host environments. Methods To identify host-dependent differentially expressed proteins, a comparative proteomic analysis of the excretory/secretory products released from E. caproni adults, isolated from hosts displaying different compatibility with this trematode, was performed. Results A total of 19 differential protein spots were identified (14 overexpressed in mice and 5 overexpressed in rats). The establishment of chronic infections in mice is mainly associated with the overexpression by adult worms of antioxidant and detoxifying enzymes (e.g. glutathione S-transferase, hydroxyacylglutathione hydrolase, thiopurine S-transferase, etc.) and metabolic enzymes like enolase, leucine aminopeptidase or malate dehydrogenase. However, the overexpression of cathepsin L and the structural protein actin observed in worms isolated from rats seems not to be effective for the colonization of the intestinal mucosa of this host. Conclusions The observed differences suggest that protein expression and/or release is modulated by the local environment generated inside the host and provide useful insights in regards to the resistance mechanisms developed by parasites to ensure their long-term survival. Helminth (dpeaa)DE-He213 Excretory/secretory products (dpeaa)DE-He213 Proteome plasticity (dpeaa)DE-He213 2-dimensional gel electrophoresis (dpeaa)DE-He213 Sotillo, Javier aut Muñoz-Antolí, Carla aut Trelis, María aut Esteban, J. Guillermo aut Toledo, Rafael aut Enthalten in Parasites & vectors London : BioMed Central, 2008 9(2016), 1 vom: 31. März (DE-627)558690076 (DE-600)2409480-8 1756-3305 nnns volume:9 year:2016 number:1 day:31 month:03 https://dx.doi.org/10.1186/s13071-016-1465-x kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2016 1 31 03
allfieldsSound	10.1186/s13071-016-1465-x doi (DE-627)SPR030841356 (SPR)s13071-016-1465-x-e DE-627 ger DE-627 rakwb eng Cortés, Alba verfasserin aut Definitive host influences the proteomic profile of excretory/secretory products of the trematode Echinostoma caproni 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Cortés et al. 2016 Background Echinostoma caproni is an intestinal trematode extensively used as experimental model for the study of factors that determine the course of intestinal helminth infections, since this markedly depends on the host species. Although the host-dependent mechanisms for either chronic establishment or early parasite rejection have been broadly studied, little is known regarding the parasite response against different host environments. Methods To identify host-dependent differentially expressed proteins, a comparative proteomic analysis of the excretory/secretory products released from E. caproni adults, isolated from hosts displaying different compatibility with this trematode, was performed. Results A total of 19 differential protein spots were identified (14 overexpressed in mice and 5 overexpressed in rats). The establishment of chronic infections in mice is mainly associated with the overexpression by adult worms of antioxidant and detoxifying enzymes (e.g. glutathione S-transferase, hydroxyacylglutathione hydrolase, thiopurine S-transferase, etc.) and metabolic enzymes like enolase, leucine aminopeptidase or malate dehydrogenase. However, the overexpression of cathepsin L and the structural protein actin observed in worms isolated from rats seems not to be effective for the colonization of the intestinal mucosa of this host. Conclusions The observed differences suggest that protein expression and/or release is modulated by the local environment generated inside the host and provide useful insights in regards to the resistance mechanisms developed by parasites to ensure their long-term survival. Helminth (dpeaa)DE-He213 Excretory/secretory products (dpeaa)DE-He213 Proteome plasticity (dpeaa)DE-He213 2-dimensional gel electrophoresis (dpeaa)DE-He213 Sotillo, Javier aut Muñoz-Antolí, Carla aut Trelis, María aut Esteban, J. Guillermo aut Toledo, Rafael aut Enthalten in Parasites & vectors London : BioMed Central, 2008 9(2016), 1 vom: 31. März (DE-627)558690076 (DE-600)2409480-8 1756-3305 nnns volume:9 year:2016 number:1 day:31 month:03 https://dx.doi.org/10.1186/s13071-016-1465-x kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2016 1 31 03
language	English
source	Enthalten in Parasites & vectors 9(2016), 1 vom: 31. März volume:9 year:2016 number:1 day:31 month:03
sourceStr	Enthalten in Parasites & vectors 9(2016), 1 vom: 31. März volume:9 year:2016 number:1 day:31 month:03
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Helminth Excretory/secretory products Proteome plasticity 2-dimensional gel electrophoresis
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container_title	Parasites & vectors
authorswithroles_txt_mv	Cortés, Alba @@aut@@ Sotillo, Javier @@aut@@ Muñoz-Antolí, Carla @@aut@@ Trelis, María @@aut@@ Esteban, J. Guillermo @@aut@@ Toledo, Rafael @@aut@@
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However, the overexpression of cathepsin L and the structural protein actin observed in worms isolated from rats seems not to be effective for the colonization of the intestinal mucosa of this host. 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author	Cortés, Alba
spellingShingle	Cortés, Alba misc Helminth misc Excretory/secretory products misc Proteome plasticity misc 2-dimensional gel electrophoresis Definitive host influences the proteomic profile of excretory/secretory products of the trematode Echinostoma caproni
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topic_title	Definitive host influences the proteomic profile of excretory/secretory products of the trematode Echinostoma caproni Helminth (dpeaa)DE-He213 Excretory/secretory products (dpeaa)DE-He213 Proteome plasticity (dpeaa)DE-He213 2-dimensional gel electrophoresis (dpeaa)DE-He213
topic	misc Helminth misc Excretory/secretory products misc Proteome plasticity misc 2-dimensional gel electrophoresis
topic_unstemmed	misc Helminth misc Excretory/secretory products misc Proteome plasticity misc 2-dimensional gel electrophoresis
topic_browse	misc Helminth misc Excretory/secretory products misc Proteome plasticity misc 2-dimensional gel electrophoresis
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title	Definitive host influences the proteomic profile of excretory/secretory products of the trematode Echinostoma caproni
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title_full	Definitive host influences the proteomic profile of excretory/secretory products of the trematode Echinostoma caproni
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author_browse	Cortés, Alba Sotillo, Javier Muñoz-Antolí, Carla Trelis, María Esteban, J. Guillermo Toledo, Rafael
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title_sort	definitive host influences the proteomic profile of excretory/secretory products of the trematode echinostoma caproni
title_auth	Definitive host influences the proteomic profile of excretory/secretory products of the trematode Echinostoma caproni
abstract	Background Echinostoma caproni is an intestinal trematode extensively used as experimental model for the study of factors that determine the course of intestinal helminth infections, since this markedly depends on the host species. Although the host-dependent mechanisms for either chronic establishment or early parasite rejection have been broadly studied, little is known regarding the parasite response against different host environments. Methods To identify host-dependent differentially expressed proteins, a comparative proteomic analysis of the excretory/secretory products released from E. caproni adults, isolated from hosts displaying different compatibility with this trematode, was performed. Results A total of 19 differential protein spots were identified (14 overexpressed in mice and 5 overexpressed in rats). The establishment of chronic infections in mice is mainly associated with the overexpression by adult worms of antioxidant and detoxifying enzymes (e.g. glutathione S-transferase, hydroxyacylglutathione hydrolase, thiopurine S-transferase, etc.) and metabolic enzymes like enolase, leucine aminopeptidase or malate dehydrogenase. However, the overexpression of cathepsin L and the structural protein actin observed in worms isolated from rats seems not to be effective for the colonization of the intestinal mucosa of this host. Conclusions The observed differences suggest that protein expression and/or release is modulated by the local environment generated inside the host and provide useful insights in regards to the resistance mechanisms developed by parasites to ensure their long-term survival. © Cortés et al. 2016
abstractGer	Background Echinostoma caproni is an intestinal trematode extensively used as experimental model for the study of factors that determine the course of intestinal helminth infections, since this markedly depends on the host species. Although the host-dependent mechanisms for either chronic establishment or early parasite rejection have been broadly studied, little is known regarding the parasite response against different host environments. Methods To identify host-dependent differentially expressed proteins, a comparative proteomic analysis of the excretory/secretory products released from E. caproni adults, isolated from hosts displaying different compatibility with this trematode, was performed. Results A total of 19 differential protein spots were identified (14 overexpressed in mice and 5 overexpressed in rats). The establishment of chronic infections in mice is mainly associated with the overexpression by adult worms of antioxidant and detoxifying enzymes (e.g. glutathione S-transferase, hydroxyacylglutathione hydrolase, thiopurine S-transferase, etc.) and metabolic enzymes like enolase, leucine aminopeptidase or malate dehydrogenase. However, the overexpression of cathepsin L and the structural protein actin observed in worms isolated from rats seems not to be effective for the colonization of the intestinal mucosa of this host. Conclusions The observed differences suggest that protein expression and/or release is modulated by the local environment generated inside the host and provide useful insights in regards to the resistance mechanisms developed by parasites to ensure their long-term survival. © Cortés et al. 2016
abstract_unstemmed	Background Echinostoma caproni is an intestinal trematode extensively used as experimental model for the study of factors that determine the course of intestinal helminth infections, since this markedly depends on the host species. Although the host-dependent mechanisms for either chronic establishment or early parasite rejection have been broadly studied, little is known regarding the parasite response against different host environments. Methods To identify host-dependent differentially expressed proteins, a comparative proteomic analysis of the excretory/secretory products released from E. caproni adults, isolated from hosts displaying different compatibility with this trematode, was performed. Results A total of 19 differential protein spots were identified (14 overexpressed in mice and 5 overexpressed in rats). The establishment of chronic infections in mice is mainly associated with the overexpression by adult worms of antioxidant and detoxifying enzymes (e.g. glutathione S-transferase, hydroxyacylglutathione hydrolase, thiopurine S-transferase, etc.) and metabolic enzymes like enolase, leucine aminopeptidase or malate dehydrogenase. However, the overexpression of cathepsin L and the structural protein actin observed in worms isolated from rats seems not to be effective for the colonization of the intestinal mucosa of this host. Conclusions The observed differences suggest that protein expression and/or release is modulated by the local environment generated inside the host and provide useful insights in regards to the resistance mechanisms developed by parasites to ensure their long-term survival. © Cortés et al. 2016
collection_details	GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700
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title_short	Definitive host influences the proteomic profile of excretory/secretory products of the trematode Echinostoma caproni
url	https://dx.doi.org/10.1186/s13071-016-1465-x
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author2	Sotillo, Javier Muñoz-Antolí, Carla Trelis, María Esteban, J. Guillermo Toledo, Rafael
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up_date	2024-07-03T20:25:53.135Z
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Definitive host influences the proteomic profile of excretory/secretory products of the trematode Echinostoma caproni

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