A protein chip based inhibitor screening for influenza neuraminidases: the importance of glycan-specific recognition

Abstract A protein chip assay system based on a specific carbohydrate-protein interaction has been constructed for the identification of chemical inhibitors of influenza neuraminidase. The devised chip is composed of three layered proteins, that is, a matrix, a probe, and a neuraminidase protein. Tr...
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Autor*in:	Lee, Jung-kyu [verfasserIn] Park, Chan-Won Kwon, Hyuk-Ku Jung, Seunho Jeong, Hyun-Ja Kang, In-Cheol Choi, Youngjin

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2014

Schlagwörter:	Carbohydrate-protein interaction Drug screening Neuraminidase Protein chip Anti-viral

Anmerkung:	© The Korean BioChip Society and Springer-Verlag Berlin Heidelberg 2014

Übergeordnetes Werk:	Enthalten in: BioChip journal - Seoul : Soc., 2007, 8(2014), 3 vom: Sept., Seite 209-217
Übergeordnetes Werk:	volume:8 ; year:2014 ; number:3 ; month:09 ; pages:209-217

Links:	Volltext

DOI / URN:	10.1007/s13206-014-8307-8

Katalog-ID:	SPR030869269

Internformat


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245	1	2	\|a A protein chip based inhibitor screening for influenza neuraminidases: the importance of glycan-specific recognition
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520			\|a Abstract A protein chip assay system based on a specific carbohydrate-protein interaction has been constructed for the identification of chemical inhibitors of influenza neuraminidase. The devised chip is composed of three layered proteins, that is, a matrix, a probe, and a neuraminidase protein. Transferrin, the matrix protein, was immobilized onto a chip panel to provide biological glycan chains. Fluorescence-labeled lectin or hemagglutinin was added as a glycan-interacting protein probe. Finally specific sialic acid moiety on the transferrin was then cleaved by an avian or human influenza neuraminidase. Each lectin or hemagglutinin showed different glycan specificity as a result of catalytic action of different influenza neuraminidase. Using this principle, we finally tested the neuraminidase of strain H1N1 as a model system to verify the screening capability of this protein chip approach.
650		4	\|a Carbohydrate-protein interaction \|7 (dpeaa)DE-He213
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650		4	\|a Protein chip \|7 (dpeaa)DE-He213
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700	1		\|a Park, Chan-Won \|4 aut
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700	1		\|a Choi, Youngjin \|4 aut
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912			\|a GBV_USEFLAG_A
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912			\|a GBV_ILN_20
912			\|a GBV_ILN_22
912			\|a GBV_ILN_23
912			\|a GBV_ILN_24
912			\|a GBV_ILN_31
912			\|a GBV_ILN_32
912			\|a GBV_ILN_39
912			\|a GBV_ILN_40
912			\|a GBV_ILN_60
912			\|a GBV_ILN_62
912			\|a GBV_ILN_63
912			\|a GBV_ILN_65
912			\|a GBV_ILN_69
912			\|a GBV_ILN_70
912			\|a GBV_ILN_73
912			\|a GBV_ILN_74
912			\|a GBV_ILN_90
912			\|a GBV_ILN_95
912			\|a GBV_ILN_100
912			\|a GBV_ILN_101
912			\|a GBV_ILN_105
912			\|a GBV_ILN_110
912			\|a GBV_ILN_120
912			\|a GBV_ILN_138
912			\|a GBV_ILN_150
912			\|a GBV_ILN_151
912			\|a GBV_ILN_161
912			\|a GBV_ILN_170
912			\|a GBV_ILN_171
912			\|a GBV_ILN_187
912			\|a GBV_ILN_213
912			\|a GBV_ILN_224
912			\|a GBV_ILN_230
912			\|a GBV_ILN_250
912			\|a GBV_ILN_281
912			\|a GBV_ILN_285
912			\|a GBV_ILN_293
912			\|a GBV_ILN_370
912			\|a GBV_ILN_602
912			\|a GBV_ILN_636
912			\|a GBV_ILN_702
912			\|a GBV_ILN_2001
912			\|a GBV_ILN_2003
912			\|a GBV_ILN_2004
912			\|a GBV_ILN_2005
912			\|a GBV_ILN_2006
912			\|a GBV_ILN_2007
912			\|a GBV_ILN_2008
912			\|a GBV_ILN_2009
912			\|a GBV_ILN_2010
912			\|a GBV_ILN_2011
912			\|a GBV_ILN_2014
912			\|a GBV_ILN_2015
912			\|a GBV_ILN_2020
912			\|a GBV_ILN_2021
912			\|a GBV_ILN_2025
912			\|a GBV_ILN_2026
912			\|a GBV_ILN_2027
912			\|a GBV_ILN_2031
912			\|a GBV_ILN_2034
912			\|a GBV_ILN_2037
912			\|a GBV_ILN_2038
912			\|a GBV_ILN_2039
912			\|a GBV_ILN_2044
912			\|a GBV_ILN_2048
912			\|a GBV_ILN_2049
912			\|a GBV_ILN_2050
912			\|a GBV_ILN_2055
912			\|a GBV_ILN_2057
912			\|a GBV_ILN_2059
912			\|a GBV_ILN_2061
912			\|a GBV_ILN_2064
912			\|a GBV_ILN_2065
912			\|a GBV_ILN_2068
912			\|a GBV_ILN_2070
912			\|a GBV_ILN_2086
912			\|a GBV_ILN_2088
912			\|a GBV_ILN_2093
912			\|a GBV_ILN_2106
912			\|a GBV_ILN_2107
912			\|a GBV_ILN_2108
912			\|a GBV_ILN_2110
912			\|a GBV_ILN_2111
912			\|a GBV_ILN_2112
912			\|a GBV_ILN_2113
912			\|a GBV_ILN_2116
912			\|a GBV_ILN_2118
912			\|a GBV_ILN_2119
912			\|a GBV_ILN_2122
912			\|a GBV_ILN_2129
912			\|a GBV_ILN_2143
912			\|a GBV_ILN_2144
912			\|a GBV_ILN_2147
912			\|a GBV_ILN_2148
912			\|a GBV_ILN_2152
912			\|a GBV_ILN_2153
912			\|a GBV_ILN_2188
912			\|a GBV_ILN_2232
912			\|a GBV_ILN_2336
912			\|a GBV_ILN_2446
912			\|a GBV_ILN_2470
912			\|a GBV_ILN_2472
912			\|a GBV_ILN_2507
912			\|a GBV_ILN_2522
912			\|a GBV_ILN_2548
912			\|a GBV_ILN_4035
912			\|a GBV_ILN_4037
912			\|a GBV_ILN_4046
912			\|a GBV_ILN_4112
912			\|a GBV_ILN_4125
912			\|a GBV_ILN_4242
912			\|a GBV_ILN_4246
912			\|a GBV_ILN_4249
912			\|a GBV_ILN_4251
912			\|a GBV_ILN_4305
912			\|a GBV_ILN_4306
912			\|a GBV_ILN_4307
912			\|a GBV_ILN_4313
912			\|a GBV_ILN_4322
912			\|a GBV_ILN_4323
912			\|a GBV_ILN_4324
912			\|a GBV_ILN_4325
912			\|a GBV_ILN_4326
912			\|a GBV_ILN_4333
912			\|a GBV_ILN_4334
912			\|a GBV_ILN_4335
912			\|a GBV_ILN_4336
912			\|a GBV_ILN_4338
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Indexfelder

author_variant	j k l jkl c w p cwp h k k hkk s j sj h j j hjj i c k ick y c yc
matchkey_str	article:20927843:2014----::poenhpaeihbtrcennfrnleznuaiiaetemotne
hierarchy_sort_str	2014
publishDate	2014
allfields	10.1007/s13206-014-8307-8 doi (DE-627)SPR030869269 (SPR)s13206-014-8307-8-e DE-627 ger DE-627 rakwb eng Lee, Jung-kyu verfasserin aut A protein chip based inhibitor screening for influenza neuraminidases: the importance of glycan-specific recognition 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Korean BioChip Society and Springer-Verlag Berlin Heidelberg 2014 Abstract A protein chip assay system based on a specific carbohydrate-protein interaction has been constructed for the identification of chemical inhibitors of influenza neuraminidase. The devised chip is composed of three layered proteins, that is, a matrix, a probe, and a neuraminidase protein. Transferrin, the matrix protein, was immobilized onto a chip panel to provide biological glycan chains. Fluorescence-labeled lectin or hemagglutinin was added as a glycan-interacting protein probe. Finally specific sialic acid moiety on the transferrin was then cleaved by an avian or human influenza neuraminidase. Each lectin or hemagglutinin showed different glycan specificity as a result of catalytic action of different influenza neuraminidase. Using this principle, we finally tested the neuraminidase of strain H1N1 as a model system to verify the screening capability of this protein chip approach. Carbohydrate-protein interaction (dpeaa)DE-He213 Drug screening (dpeaa)DE-He213 Neuraminidase (dpeaa)DE-He213 Protein chip (dpeaa)DE-He213 Anti-viral (dpeaa)DE-He213 Park, Chan-Won aut Kwon, Hyuk-Ku aut Jung, Seunho aut Jeong, Hyun-Ja aut Kang, In-Cheol aut Choi, Youngjin aut Enthalten in BioChip journal Seoul : Soc., 2007 8(2014), 3 vom: Sept., Seite 209-217 (DE-627)608497258 (DE-600)2513796-7 2092-7843 nnns volume:8 year:2014 number:3 month:09 pages:209-217 https://dx.doi.org/10.1007/s13206-014-8307-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 8 2014 3 09 209-217
spelling	10.1007/s13206-014-8307-8 doi (DE-627)SPR030869269 (SPR)s13206-014-8307-8-e DE-627 ger DE-627 rakwb eng Lee, Jung-kyu verfasserin aut A protein chip based inhibitor screening for influenza neuraminidases: the importance of glycan-specific recognition 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Korean BioChip Society and Springer-Verlag Berlin Heidelberg 2014 Abstract A protein chip assay system based on a specific carbohydrate-protein interaction has been constructed for the identification of chemical inhibitors of influenza neuraminidase. The devised chip is composed of three layered proteins, that is, a matrix, a probe, and a neuraminidase protein. Transferrin, the matrix protein, was immobilized onto a chip panel to provide biological glycan chains. Fluorescence-labeled lectin or hemagglutinin was added as a glycan-interacting protein probe. Finally specific sialic acid moiety on the transferrin was then cleaved by an avian or human influenza neuraminidase. Each lectin or hemagglutinin showed different glycan specificity as a result of catalytic action of different influenza neuraminidase. Using this principle, we finally tested the neuraminidase of strain H1N1 as a model system to verify the screening capability of this protein chip approach. Carbohydrate-protein interaction (dpeaa)DE-He213 Drug screening (dpeaa)DE-He213 Neuraminidase (dpeaa)DE-He213 Protein chip (dpeaa)DE-He213 Anti-viral (dpeaa)DE-He213 Park, Chan-Won aut Kwon, Hyuk-Ku aut Jung, Seunho aut Jeong, Hyun-Ja aut Kang, In-Cheol aut Choi, Youngjin aut Enthalten in BioChip journal Seoul : Soc., 2007 8(2014), 3 vom: Sept., Seite 209-217 (DE-627)608497258 (DE-600)2513796-7 2092-7843 nnns volume:8 year:2014 number:3 month:09 pages:209-217 https://dx.doi.org/10.1007/s13206-014-8307-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 8 2014 3 09 209-217
allfields_unstemmed	10.1007/s13206-014-8307-8 doi (DE-627)SPR030869269 (SPR)s13206-014-8307-8-e DE-627 ger DE-627 rakwb eng Lee, Jung-kyu verfasserin aut A protein chip based inhibitor screening for influenza neuraminidases: the importance of glycan-specific recognition 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Korean BioChip Society and Springer-Verlag Berlin Heidelberg 2014 Abstract A protein chip assay system based on a specific carbohydrate-protein interaction has been constructed for the identification of chemical inhibitors of influenza neuraminidase. The devised chip is composed of three layered proteins, that is, a matrix, a probe, and a neuraminidase protein. Transferrin, the matrix protein, was immobilized onto a chip panel to provide biological glycan chains. Fluorescence-labeled lectin or hemagglutinin was added as a glycan-interacting protein probe. Finally specific sialic acid moiety on the transferrin was then cleaved by an avian or human influenza neuraminidase. Each lectin or hemagglutinin showed different glycan specificity as a result of catalytic action of different influenza neuraminidase. Using this principle, we finally tested the neuraminidase of strain H1N1 as a model system to verify the screening capability of this protein chip approach. Carbohydrate-protein interaction (dpeaa)DE-He213 Drug screening (dpeaa)DE-He213 Neuraminidase (dpeaa)DE-He213 Protein chip (dpeaa)DE-He213 Anti-viral (dpeaa)DE-He213 Park, Chan-Won aut Kwon, Hyuk-Ku aut Jung, Seunho aut Jeong, Hyun-Ja aut Kang, In-Cheol aut Choi, Youngjin aut Enthalten in BioChip journal Seoul : Soc., 2007 8(2014), 3 vom: Sept., Seite 209-217 (DE-627)608497258 (DE-600)2513796-7 2092-7843 nnns volume:8 year:2014 number:3 month:09 pages:209-217 https://dx.doi.org/10.1007/s13206-014-8307-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 8 2014 3 09 209-217
allfieldsGer	10.1007/s13206-014-8307-8 doi (DE-627)SPR030869269 (SPR)s13206-014-8307-8-e DE-627 ger DE-627 rakwb eng Lee, Jung-kyu verfasserin aut A protein chip based inhibitor screening for influenza neuraminidases: the importance of glycan-specific recognition 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Korean BioChip Society and Springer-Verlag Berlin Heidelberg 2014 Abstract A protein chip assay system based on a specific carbohydrate-protein interaction has been constructed for the identification of chemical inhibitors of influenza neuraminidase. The devised chip is composed of three layered proteins, that is, a matrix, a probe, and a neuraminidase protein. Transferrin, the matrix protein, was immobilized onto a chip panel to provide biological glycan chains. Fluorescence-labeled lectin or hemagglutinin was added as a glycan-interacting protein probe. Finally specific sialic acid moiety on the transferrin was then cleaved by an avian or human influenza neuraminidase. Each lectin or hemagglutinin showed different glycan specificity as a result of catalytic action of different influenza neuraminidase. Using this principle, we finally tested the neuraminidase of strain H1N1 as a model system to verify the screening capability of this protein chip approach. Carbohydrate-protein interaction (dpeaa)DE-He213 Drug screening (dpeaa)DE-He213 Neuraminidase (dpeaa)DE-He213 Protein chip (dpeaa)DE-He213 Anti-viral (dpeaa)DE-He213 Park, Chan-Won aut Kwon, Hyuk-Ku aut Jung, Seunho aut Jeong, Hyun-Ja aut Kang, In-Cheol aut Choi, Youngjin aut Enthalten in BioChip journal Seoul : Soc., 2007 8(2014), 3 vom: Sept., Seite 209-217 (DE-627)608497258 (DE-600)2513796-7 2092-7843 nnns volume:8 year:2014 number:3 month:09 pages:209-217 https://dx.doi.org/10.1007/s13206-014-8307-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 8 2014 3 09 209-217
allfieldsSound	10.1007/s13206-014-8307-8 doi (DE-627)SPR030869269 (SPR)s13206-014-8307-8-e DE-627 ger DE-627 rakwb eng Lee, Jung-kyu verfasserin aut A protein chip based inhibitor screening for influenza neuraminidases: the importance of glycan-specific recognition 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Korean BioChip Society and Springer-Verlag Berlin Heidelberg 2014 Abstract A protein chip assay system based on a specific carbohydrate-protein interaction has been constructed for the identification of chemical inhibitors of influenza neuraminidase. The devised chip is composed of three layered proteins, that is, a matrix, a probe, and a neuraminidase protein. Transferrin, the matrix protein, was immobilized onto a chip panel to provide biological glycan chains. Fluorescence-labeled lectin or hemagglutinin was added as a glycan-interacting protein probe. Finally specific sialic acid moiety on the transferrin was then cleaved by an avian or human influenza neuraminidase. Each lectin or hemagglutinin showed different glycan specificity as a result of catalytic action of different influenza neuraminidase. Using this principle, we finally tested the neuraminidase of strain H1N1 as a model system to verify the screening capability of this protein chip approach. Carbohydrate-protein interaction (dpeaa)DE-He213 Drug screening (dpeaa)DE-He213 Neuraminidase (dpeaa)DE-He213 Protein chip (dpeaa)DE-He213 Anti-viral (dpeaa)DE-He213 Park, Chan-Won aut Kwon, Hyuk-Ku aut Jung, Seunho aut Jeong, Hyun-Ja aut Kang, In-Cheol aut Choi, Youngjin aut Enthalten in BioChip journal Seoul : Soc., 2007 8(2014), 3 vom: Sept., Seite 209-217 (DE-627)608497258 (DE-600)2513796-7 2092-7843 nnns volume:8 year:2014 number:3 month:09 pages:209-217 https://dx.doi.org/10.1007/s13206-014-8307-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 8 2014 3 09 209-217
language	English
source	Enthalten in BioChip journal 8(2014), 3 vom: Sept., Seite 209-217 volume:8 year:2014 number:3 month:09 pages:209-217
sourceStr	Enthalten in BioChip journal 8(2014), 3 vom: Sept., Seite 209-217 volume:8 year:2014 number:3 month:09 pages:209-217
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Carbohydrate-protein interaction Drug screening Neuraminidase Protein chip Anti-viral
isfreeaccess_bool	false
container_title	BioChip journal
authorswithroles_txt_mv	Lee, Jung-kyu @@aut@@ Park, Chan-Won @@aut@@ Kwon, Hyuk-Ku @@aut@@ Jung, Seunho @@aut@@ Jeong, Hyun-Ja @@aut@@ Kang, In-Cheol @@aut@@ Choi, Youngjin @@aut@@
publishDateDaySort_date	2014-09-01T00:00:00Z
hierarchy_top_id	608497258
id	SPR030869269
language_de	englisch
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author	Lee, Jung-kyu
spellingShingle	Lee, Jung-kyu misc Carbohydrate-protein interaction misc Drug screening misc Neuraminidase misc Protein chip misc Anti-viral A protein chip based inhibitor screening for influenza neuraminidases: the importance of glycan-specific recognition
authorStr	Lee, Jung-kyu
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topic_title	A protein chip based inhibitor screening for influenza neuraminidases: the importance of glycan-specific recognition Carbohydrate-protein interaction (dpeaa)DE-He213 Drug screening (dpeaa)DE-He213 Neuraminidase (dpeaa)DE-He213 Protein chip (dpeaa)DE-He213 Anti-viral (dpeaa)DE-He213
topic	misc Carbohydrate-protein interaction misc Drug screening misc Neuraminidase misc Protein chip misc Anti-viral
topic_unstemmed	misc Carbohydrate-protein interaction misc Drug screening misc Neuraminidase misc Protein chip misc Anti-viral
topic_browse	misc Carbohydrate-protein interaction misc Drug screening misc Neuraminidase misc Protein chip misc Anti-viral
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title	A protein chip based inhibitor screening for influenza neuraminidases: the importance of glycan-specific recognition
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title_full	A protein chip based inhibitor screening for influenza neuraminidases: the importance of glycan-specific recognition
author_sort	Lee, Jung-kyu
journal	BioChip journal
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container_start_page	209
author_browse	Lee, Jung-kyu Park, Chan-Won Kwon, Hyuk-Ku Jung, Seunho Jeong, Hyun-Ja Kang, In-Cheol Choi, Youngjin
container_volume	8
format_se	Elektronische Aufsätze
author-letter	Lee, Jung-kyu
doi_str_mv	10.1007/s13206-014-8307-8
title_sort	protein chip based inhibitor screening for influenza neuraminidases: the importance of glycan-specific recognition
title_auth	A protein chip based inhibitor screening for influenza neuraminidases: the importance of glycan-specific recognition
abstract	Abstract A protein chip assay system based on a specific carbohydrate-protein interaction has been constructed for the identification of chemical inhibitors of influenza neuraminidase. The devised chip is composed of three layered proteins, that is, a matrix, a probe, and a neuraminidase protein. Transferrin, the matrix protein, was immobilized onto a chip panel to provide biological glycan chains. Fluorescence-labeled lectin or hemagglutinin was added as a glycan-interacting protein probe. Finally specific sialic acid moiety on the transferrin was then cleaved by an avian or human influenza neuraminidase. Each lectin or hemagglutinin showed different glycan specificity as a result of catalytic action of different influenza neuraminidase. Using this principle, we finally tested the neuraminidase of strain H1N1 as a model system to verify the screening capability of this protein chip approach. © The Korean BioChip Society and Springer-Verlag Berlin Heidelberg 2014
abstractGer	Abstract A protein chip assay system based on a specific carbohydrate-protein interaction has been constructed for the identification of chemical inhibitors of influenza neuraminidase. The devised chip is composed of three layered proteins, that is, a matrix, a probe, and a neuraminidase protein. Transferrin, the matrix protein, was immobilized onto a chip panel to provide biological glycan chains. Fluorescence-labeled lectin or hemagglutinin was added as a glycan-interacting protein probe. Finally specific sialic acid moiety on the transferrin was then cleaved by an avian or human influenza neuraminidase. Each lectin or hemagglutinin showed different glycan specificity as a result of catalytic action of different influenza neuraminidase. Using this principle, we finally tested the neuraminidase of strain H1N1 as a model system to verify the screening capability of this protein chip approach. © The Korean BioChip Society and Springer-Verlag Berlin Heidelberg 2014
abstract_unstemmed	Abstract A protein chip assay system based on a specific carbohydrate-protein interaction has been constructed for the identification of chemical inhibitors of influenza neuraminidase. The devised chip is composed of three layered proteins, that is, a matrix, a probe, and a neuraminidase protein. Transferrin, the matrix protein, was immobilized onto a chip panel to provide biological glycan chains. Fluorescence-labeled lectin or hemagglutinin was added as a glycan-interacting protein probe. Finally specific sialic acid moiety on the transferrin was then cleaved by an avian or human influenza neuraminidase. Each lectin or hemagglutinin showed different glycan specificity as a result of catalytic action of different influenza neuraminidase. Using this principle, we finally tested the neuraminidase of strain H1N1 as a model system to verify the screening capability of this protein chip approach. © The Korean BioChip Society and Springer-Verlag Berlin Heidelberg 2014
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container_issue	3
title_short	A protein chip based inhibitor screening for influenza neuraminidases: the importance of glycan-specific recognition
url	https://dx.doi.org/10.1007/s13206-014-8307-8
remote_bool	true
author2	Park, Chan-Won Kwon, Hyuk-Ku Jung, Seunho Jeong, Hyun-Ja Kang, In-Cheol Choi, Youngjin
author2Str	Park, Chan-Won Kwon, Hyuk-Ku Jung, Seunho Jeong, Hyun-Ja Kang, In-Cheol Choi, Youngjin
ppnlink	608497258
mediatype_str_mv	c
isOA_txt	false
hochschulschrift_bool	false
doi_str	10.1007/s13206-014-8307-8
up_date	2024-07-03T20:36:50.910Z
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