Measurement of prostate-specific antigen level as a biomarker for breast cancer by using mass signal amplification
Abstract Recently, it has been known that prostatespecific antigen (PSA) can be a useful biomarker for the diagnosis with breast cancer as well as that with prostate cancer. The level of PSA in patients of breast cancer is much lower than that in patients of prostate cancer, and therefore, the quant...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Nam, Jungchan [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2015 |
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| Schlagwörter: |
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| Anmerkung: |
© The Korean BioChip Society and Springer-Verlag Berlin Heidelberg 2015 |
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| Übergeordnetes Werk: |
Enthalten in: BioChip journal - Seoul : Soc., 2007, 9(2015), 2 vom: 27. März, Seite 124-129 |
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| Übergeordnetes Werk: |
volume:9 ; year:2015 ; number:2 ; day:27 ; month:03 ; pages:124-129 |
| Links: |
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| DOI / URN: |
10.1007/s13206-015-9205-4 |
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| Katalog-ID: |
SPR030869579 |
|---|
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| 100 | 1 | |a Nam, Jungchan |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Measurement of prostate-specific antigen level as a biomarker for breast cancer by using mass signal amplification |
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| 520 | |a Abstract Recently, it has been known that prostatespecific antigen (PSA) can be a useful biomarker for the diagnosis with breast cancer as well as that with prostate cancer. The level of PSA in patients of breast cancer is much lower than that in patients of prostate cancer, and therefore, the quantitative method of PSA with ultra-high sensitivity is required for the breast cancer diagnosis. In this paper, we introduce an efficient method for selective detection and quantification of PSA using gold nanoparticles (AuNPs), biochips, and mass spectrometry (MS). The PSA in a sample was captured by AuNPs and a biochip which present antibodies through a sandwich assay format. The AuNPs carried a huge number of reporter molecules, called amplification tag (Am-tag), and the presence of PSA in the sample was then verified through amplified mass signal of Am-tag by laser desorption/ionization timeof- flight (LDI-TOF) MS analysis to enable ultrasensitive detection of PSA. In addition, PSA in the sample was quantitated by using an internal standard (IS) which was prepared with AuNPs and deuterium-labeled Amtag (dAm-tag) which has a heavier molecular weight. Therefore, comparing the mass intensities between the Am-tag and the dAm-tag allowed quantification of PSA. This method was validated through the quantification of PSA in the female human serums with satisfactory results. We believe our method to detect and analyze PSA quantitatively and highly sensitively will be applied to patients’ serum for the diagnosis of breast cancer. | ||
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| 700 | 1 | |a Yoo, Minyoung |4 aut | |
| 700 | 1 | |a Yeo, Woon-Seok |4 aut | |
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10.1007/s13206-015-9205-4 doi (DE-627)SPR030869579 (SPR)s13206-015-9205-4-e DE-627 ger DE-627 rakwb eng Nam, Jungchan verfasserin aut Measurement of prostate-specific antigen level as a biomarker for breast cancer by using mass signal amplification 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Korean BioChip Society and Springer-Verlag Berlin Heidelberg 2015 Abstract Recently, it has been known that prostatespecific antigen (PSA) can be a useful biomarker for the diagnosis with breast cancer as well as that with prostate cancer. The level of PSA in patients of breast cancer is much lower than that in patients of prostate cancer, and therefore, the quantitative method of PSA with ultra-high sensitivity is required for the breast cancer diagnosis. In this paper, we introduce an efficient method for selective detection and quantification of PSA using gold nanoparticles (AuNPs), biochips, and mass spectrometry (MS). The PSA in a sample was captured by AuNPs and a biochip which present antibodies through a sandwich assay format. The AuNPs carried a huge number of reporter molecules, called amplification tag (Am-tag), and the presence of PSA in the sample was then verified through amplified mass signal of Am-tag by laser desorption/ionization timeof- flight (LDI-TOF) MS analysis to enable ultrasensitive detection of PSA. In addition, PSA in the sample was quantitated by using an internal standard (IS) which was prepared with AuNPs and deuterium-labeled Amtag (dAm-tag) which has a heavier molecular weight. Therefore, comparing the mass intensities between the Am-tag and the dAm-tag allowed quantification of PSA. This method was validated through the quantification of PSA in the female human serums with satisfactory results. We believe our method to detect and analyze PSA quantitatively and highly sensitively will be applied to patients’ serum for the diagnosis of breast cancer. Prostate-specific antigen (dpeaa)DE-He213 Gold nanoparticle (dpeaa)DE-He213 LDI-TOF MS (dpeaa)DE-He213 Self-assembled monolayers (dpeaa)DE-He213 Yoo, Minyoung aut Yeo, Woon-Seok aut Enthalten in BioChip journal Seoul : Soc., 2007 9(2015), 2 vom: 27. März, Seite 124-129 (DE-627)608497258 (DE-600)2513796-7 2092-7843 nnns volume:9 year:2015 number:2 day:27 month:03 pages:124-129 https://dx.doi.org/10.1007/s13206-015-9205-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 9 2015 2 27 03 124-129 |
| spelling |
10.1007/s13206-015-9205-4 doi (DE-627)SPR030869579 (SPR)s13206-015-9205-4-e DE-627 ger DE-627 rakwb eng Nam, Jungchan verfasserin aut Measurement of prostate-specific antigen level as a biomarker for breast cancer by using mass signal amplification 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Korean BioChip Society and Springer-Verlag Berlin Heidelberg 2015 Abstract Recently, it has been known that prostatespecific antigen (PSA) can be a useful biomarker for the diagnosis with breast cancer as well as that with prostate cancer. The level of PSA in patients of breast cancer is much lower than that in patients of prostate cancer, and therefore, the quantitative method of PSA with ultra-high sensitivity is required for the breast cancer diagnosis. In this paper, we introduce an efficient method for selective detection and quantification of PSA using gold nanoparticles (AuNPs), biochips, and mass spectrometry (MS). The PSA in a sample was captured by AuNPs and a biochip which present antibodies through a sandwich assay format. The AuNPs carried a huge number of reporter molecules, called amplification tag (Am-tag), and the presence of PSA in the sample was then verified through amplified mass signal of Am-tag by laser desorption/ionization timeof- flight (LDI-TOF) MS analysis to enable ultrasensitive detection of PSA. In addition, PSA in the sample was quantitated by using an internal standard (IS) which was prepared with AuNPs and deuterium-labeled Amtag (dAm-tag) which has a heavier molecular weight. Therefore, comparing the mass intensities between the Am-tag and the dAm-tag allowed quantification of PSA. This method was validated through the quantification of PSA in the female human serums with satisfactory results. We believe our method to detect and analyze PSA quantitatively and highly sensitively will be applied to patients’ serum for the diagnosis of breast cancer. Prostate-specific antigen (dpeaa)DE-He213 Gold nanoparticle (dpeaa)DE-He213 LDI-TOF MS (dpeaa)DE-He213 Self-assembled monolayers (dpeaa)DE-He213 Yoo, Minyoung aut Yeo, Woon-Seok aut Enthalten in BioChip journal Seoul : Soc., 2007 9(2015), 2 vom: 27. März, Seite 124-129 (DE-627)608497258 (DE-600)2513796-7 2092-7843 nnns volume:9 year:2015 number:2 day:27 month:03 pages:124-129 https://dx.doi.org/10.1007/s13206-015-9205-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 9 2015 2 27 03 124-129 |
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10.1007/s13206-015-9205-4 doi (DE-627)SPR030869579 (SPR)s13206-015-9205-4-e DE-627 ger DE-627 rakwb eng Nam, Jungchan verfasserin aut Measurement of prostate-specific antigen level as a biomarker for breast cancer by using mass signal amplification 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Korean BioChip Society and Springer-Verlag Berlin Heidelberg 2015 Abstract Recently, it has been known that prostatespecific antigen (PSA) can be a useful biomarker for the diagnosis with breast cancer as well as that with prostate cancer. The level of PSA in patients of breast cancer is much lower than that in patients of prostate cancer, and therefore, the quantitative method of PSA with ultra-high sensitivity is required for the breast cancer diagnosis. In this paper, we introduce an efficient method for selective detection and quantification of PSA using gold nanoparticles (AuNPs), biochips, and mass spectrometry (MS). The PSA in a sample was captured by AuNPs and a biochip which present antibodies through a sandwich assay format. The AuNPs carried a huge number of reporter molecules, called amplification tag (Am-tag), and the presence of PSA in the sample was then verified through amplified mass signal of Am-tag by laser desorption/ionization timeof- flight (LDI-TOF) MS analysis to enable ultrasensitive detection of PSA. In addition, PSA in the sample was quantitated by using an internal standard (IS) which was prepared with AuNPs and deuterium-labeled Amtag (dAm-tag) which has a heavier molecular weight. Therefore, comparing the mass intensities between the Am-tag and the dAm-tag allowed quantification of PSA. This method was validated through the quantification of PSA in the female human serums with satisfactory results. We believe our method to detect and analyze PSA quantitatively and highly sensitively will be applied to patients’ serum for the diagnosis of breast cancer. Prostate-specific antigen (dpeaa)DE-He213 Gold nanoparticle (dpeaa)DE-He213 LDI-TOF MS (dpeaa)DE-He213 Self-assembled monolayers (dpeaa)DE-He213 Yoo, Minyoung aut Yeo, Woon-Seok aut Enthalten in BioChip journal Seoul : Soc., 2007 9(2015), 2 vom: 27. März, Seite 124-129 (DE-627)608497258 (DE-600)2513796-7 2092-7843 nnns volume:9 year:2015 number:2 day:27 month:03 pages:124-129 https://dx.doi.org/10.1007/s13206-015-9205-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 9 2015 2 27 03 124-129 |
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10.1007/s13206-015-9205-4 doi (DE-627)SPR030869579 (SPR)s13206-015-9205-4-e DE-627 ger DE-627 rakwb eng Nam, Jungchan verfasserin aut Measurement of prostate-specific antigen level as a biomarker for breast cancer by using mass signal amplification 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Korean BioChip Society and Springer-Verlag Berlin Heidelberg 2015 Abstract Recently, it has been known that prostatespecific antigen (PSA) can be a useful biomarker for the diagnosis with breast cancer as well as that with prostate cancer. The level of PSA in patients of breast cancer is much lower than that in patients of prostate cancer, and therefore, the quantitative method of PSA with ultra-high sensitivity is required for the breast cancer diagnosis. In this paper, we introduce an efficient method for selective detection and quantification of PSA using gold nanoparticles (AuNPs), biochips, and mass spectrometry (MS). The PSA in a sample was captured by AuNPs and a biochip which present antibodies through a sandwich assay format. The AuNPs carried a huge number of reporter molecules, called amplification tag (Am-tag), and the presence of PSA in the sample was then verified through amplified mass signal of Am-tag by laser desorption/ionization timeof- flight (LDI-TOF) MS analysis to enable ultrasensitive detection of PSA. In addition, PSA in the sample was quantitated by using an internal standard (IS) which was prepared with AuNPs and deuterium-labeled Amtag (dAm-tag) which has a heavier molecular weight. Therefore, comparing the mass intensities between the Am-tag and the dAm-tag allowed quantification of PSA. This method was validated through the quantification of PSA in the female human serums with satisfactory results. We believe our method to detect and analyze PSA quantitatively and highly sensitively will be applied to patients’ serum for the diagnosis of breast cancer. Prostate-specific antigen (dpeaa)DE-He213 Gold nanoparticle (dpeaa)DE-He213 LDI-TOF MS (dpeaa)DE-He213 Self-assembled monolayers (dpeaa)DE-He213 Yoo, Minyoung aut Yeo, Woon-Seok aut Enthalten in BioChip journal Seoul : Soc., 2007 9(2015), 2 vom: 27. März, Seite 124-129 (DE-627)608497258 (DE-600)2513796-7 2092-7843 nnns volume:9 year:2015 number:2 day:27 month:03 pages:124-129 https://dx.doi.org/10.1007/s13206-015-9205-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 9 2015 2 27 03 124-129 |
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10.1007/s13206-015-9205-4 doi (DE-627)SPR030869579 (SPR)s13206-015-9205-4-e DE-627 ger DE-627 rakwb eng Nam, Jungchan verfasserin aut Measurement of prostate-specific antigen level as a biomarker for breast cancer by using mass signal amplification 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Korean BioChip Society and Springer-Verlag Berlin Heidelberg 2015 Abstract Recently, it has been known that prostatespecific antigen (PSA) can be a useful biomarker for the diagnosis with breast cancer as well as that with prostate cancer. The level of PSA in patients of breast cancer is much lower than that in patients of prostate cancer, and therefore, the quantitative method of PSA with ultra-high sensitivity is required for the breast cancer diagnosis. In this paper, we introduce an efficient method for selective detection and quantification of PSA using gold nanoparticles (AuNPs), biochips, and mass spectrometry (MS). The PSA in a sample was captured by AuNPs and a biochip which present antibodies through a sandwich assay format. The AuNPs carried a huge number of reporter molecules, called amplification tag (Am-tag), and the presence of PSA in the sample was then verified through amplified mass signal of Am-tag by laser desorption/ionization timeof- flight (LDI-TOF) MS analysis to enable ultrasensitive detection of PSA. In addition, PSA in the sample was quantitated by using an internal standard (IS) which was prepared with AuNPs and deuterium-labeled Amtag (dAm-tag) which has a heavier molecular weight. Therefore, comparing the mass intensities between the Am-tag and the dAm-tag allowed quantification of PSA. This method was validated through the quantification of PSA in the female human serums with satisfactory results. We believe our method to detect and analyze PSA quantitatively and highly sensitively will be applied to patients’ serum for the diagnosis of breast cancer. Prostate-specific antigen (dpeaa)DE-He213 Gold nanoparticle (dpeaa)DE-He213 LDI-TOF MS (dpeaa)DE-He213 Self-assembled monolayers (dpeaa)DE-He213 Yoo, Minyoung aut Yeo, Woon-Seok aut Enthalten in BioChip journal Seoul : Soc., 2007 9(2015), 2 vom: 27. März, Seite 124-129 (DE-627)608497258 (DE-600)2513796-7 2092-7843 nnns volume:9 year:2015 number:2 day:27 month:03 pages:124-129 https://dx.doi.org/10.1007/s13206-015-9205-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 9 2015 2 27 03 124-129 |
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Nam, Jungchan @@aut@@ Yoo, Minyoung @@aut@@ Yeo, Woon-Seok @@aut@@ |
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The level of PSA in patients of breast cancer is much lower than that in patients of prostate cancer, and therefore, the quantitative method of PSA with ultra-high sensitivity is required for the breast cancer diagnosis. In this paper, we introduce an efficient method for selective detection and quantification of PSA using gold nanoparticles (AuNPs), biochips, and mass spectrometry (MS). The PSA in a sample was captured by AuNPs and a biochip which present antibodies through a sandwich assay format. The AuNPs carried a huge number of reporter molecules, called amplification tag (Am-tag), and the presence of PSA in the sample was then verified through amplified mass signal of Am-tag by laser desorption/ionization timeof- flight (LDI-TOF) MS analysis to enable ultrasensitive detection of PSA. In addition, PSA in the sample was quantitated by using an internal standard (IS) which was prepared with AuNPs and deuterium-labeled Amtag (dAm-tag) which has a heavier molecular weight. Therefore, comparing the mass intensities between the Am-tag and the dAm-tag allowed quantification of PSA. This method was validated through the quantification of PSA in the female human serums with satisfactory results. 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Nam, Jungchan |
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Measurement of prostate-specific antigen level as a biomarker for breast cancer by using mass signal amplification Prostate-specific antigen (dpeaa)DE-He213 Gold nanoparticle (dpeaa)DE-He213 LDI-TOF MS (dpeaa)DE-He213 Self-assembled monolayers (dpeaa)DE-He213 |
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measurement of prostate-specific antigen level as a biomarker for breast cancer by using mass signal amplification |
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Measurement of prostate-specific antigen level as a biomarker for breast cancer by using mass signal amplification |
| abstract |
Abstract Recently, it has been known that prostatespecific antigen (PSA) can be a useful biomarker for the diagnosis with breast cancer as well as that with prostate cancer. The level of PSA in patients of breast cancer is much lower than that in patients of prostate cancer, and therefore, the quantitative method of PSA with ultra-high sensitivity is required for the breast cancer diagnosis. In this paper, we introduce an efficient method for selective detection and quantification of PSA using gold nanoparticles (AuNPs), biochips, and mass spectrometry (MS). The PSA in a sample was captured by AuNPs and a biochip which present antibodies through a sandwich assay format. The AuNPs carried a huge number of reporter molecules, called amplification tag (Am-tag), and the presence of PSA in the sample was then verified through amplified mass signal of Am-tag by laser desorption/ionization timeof- flight (LDI-TOF) MS analysis to enable ultrasensitive detection of PSA. In addition, PSA in the sample was quantitated by using an internal standard (IS) which was prepared with AuNPs and deuterium-labeled Amtag (dAm-tag) which has a heavier molecular weight. Therefore, comparing the mass intensities between the Am-tag and the dAm-tag allowed quantification of PSA. This method was validated through the quantification of PSA in the female human serums with satisfactory results. We believe our method to detect and analyze PSA quantitatively and highly sensitively will be applied to patients’ serum for the diagnosis of breast cancer. © The Korean BioChip Society and Springer-Verlag Berlin Heidelberg 2015 |
| abstractGer |
Abstract Recently, it has been known that prostatespecific antigen (PSA) can be a useful biomarker for the diagnosis with breast cancer as well as that with prostate cancer. The level of PSA in patients of breast cancer is much lower than that in patients of prostate cancer, and therefore, the quantitative method of PSA with ultra-high sensitivity is required for the breast cancer diagnosis. In this paper, we introduce an efficient method for selective detection and quantification of PSA using gold nanoparticles (AuNPs), biochips, and mass spectrometry (MS). The PSA in a sample was captured by AuNPs and a biochip which present antibodies through a sandwich assay format. The AuNPs carried a huge number of reporter molecules, called amplification tag (Am-tag), and the presence of PSA in the sample was then verified through amplified mass signal of Am-tag by laser desorption/ionization timeof- flight (LDI-TOF) MS analysis to enable ultrasensitive detection of PSA. In addition, PSA in the sample was quantitated by using an internal standard (IS) which was prepared with AuNPs and deuterium-labeled Amtag (dAm-tag) which has a heavier molecular weight. Therefore, comparing the mass intensities between the Am-tag and the dAm-tag allowed quantification of PSA. This method was validated through the quantification of PSA in the female human serums with satisfactory results. We believe our method to detect and analyze PSA quantitatively and highly sensitively will be applied to patients’ serum for the diagnosis of breast cancer. © The Korean BioChip Society and Springer-Verlag Berlin Heidelberg 2015 |
| abstract_unstemmed |
Abstract Recently, it has been known that prostatespecific antigen (PSA) can be a useful biomarker for the diagnosis with breast cancer as well as that with prostate cancer. The level of PSA in patients of breast cancer is much lower than that in patients of prostate cancer, and therefore, the quantitative method of PSA with ultra-high sensitivity is required for the breast cancer diagnosis. In this paper, we introduce an efficient method for selective detection and quantification of PSA using gold nanoparticles (AuNPs), biochips, and mass spectrometry (MS). The PSA in a sample was captured by AuNPs and a biochip which present antibodies through a sandwich assay format. The AuNPs carried a huge number of reporter molecules, called amplification tag (Am-tag), and the presence of PSA in the sample was then verified through amplified mass signal of Am-tag by laser desorption/ionization timeof- flight (LDI-TOF) MS analysis to enable ultrasensitive detection of PSA. In addition, PSA in the sample was quantitated by using an internal standard (IS) which was prepared with AuNPs and deuterium-labeled Amtag (dAm-tag) which has a heavier molecular weight. Therefore, comparing the mass intensities between the Am-tag and the dAm-tag allowed quantification of PSA. This method was validated through the quantification of PSA in the female human serums with satisfactory results. We believe our method to detect and analyze PSA quantitatively and highly sensitively will be applied to patients’ serum for the diagnosis of breast cancer. © The Korean BioChip Society and Springer-Verlag Berlin Heidelberg 2015 |
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Measurement of prostate-specific antigen level as a biomarker for breast cancer by using mass signal amplification |
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https://dx.doi.org/10.1007/s13206-015-9205-4 |
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Yoo, Minyoung Yeo, Woon-Seok |
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| score |
7.399617 |