Design and Fabrication of Miniaturized Neuronal Circuits on Microelectrode Arrays Using Agarose Hydrogel Micro-molding Technique

Abstract Dissociated neuronal cultures combined with planar-type microelectrode arrays (MEAs) have been used as a promising read-out platform for the application of cell-based biosensors. There are increasing interests in engineering neuronal cultures to form the desired network topology by surface...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Joo, Sunghoon [verfasserIn] Lim, Jisoon Nam, Yoonkey

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2018

Schlagwörter:	Neuron patterning Microelectrode arrays Surface patterning Micromolding in capillaries Long-term culture

Anmerkung:	© The Korean BioChip Society and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Übergeordnetes Werk:	Enthalten in: BioChip journal - Seoul : Soc., 2007, 12(2018), 3 vom: 10. Juli, Seite 193-201
Übergeordnetes Werk:	volume:12 ; year:2018 ; number:3 ; day:10 ; month:07 ; pages:193-201

Links:	Volltext

DOI / URN:	10.1007/s13206-018-2308-y

Katalog-ID:	SPR030871042

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520			\|a Abstract Dissociated neuronal cultures combined with planar-type microelectrode arrays (MEAs) have been used as a promising read-out platform for the application of cell-based biosensors. There are increasing interests in engineering neuronal cultures to form the desired network topology by surface micropatterning technology. Here, we report a long-term cultivation of primary hippocampal neurons on microelectrode arrays using soft-lithography. Ordered hippocampal neuronal networks were formed by seeding neurons in agarose-microwells and inducing neurite outgrowth through microgrooves. Unlike previous approaches, our technique allowed us to design networks with various microwells on microelectrode arrays with high repeatability. These hippocampal network chips were cultivated for 30 days with excellent pattern fidelity, and neural spikes were successfully measured. We also found that spontaneous activity of the networks could be enhanced by acute disinhibition of inhibitory synapses. The proposed patterning method for neuronal network chips will be a potentially powerful tool for cell-based drug-screening applications.
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matchkey_str	article:20927843:2018----::einnfbiainfiitrzdernlicisnireetoerassnaaoe
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allfields	10.1007/s13206-018-2308-y doi (DE-627)SPR030871042 (SPR)s13206-018-2308-y-e DE-627 ger DE-627 rakwb eng Joo, Sunghoon verfasserin aut Design and Fabrication of Miniaturized Neuronal Circuits on Microelectrode Arrays Using Agarose Hydrogel Micro-molding Technique 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Korean BioChip Society and Springer-Verlag GmbH Germany, part of Springer Nature 2018 Abstract Dissociated neuronal cultures combined with planar-type microelectrode arrays (MEAs) have been used as a promising read-out platform for the application of cell-based biosensors. There are increasing interests in engineering neuronal cultures to form the desired network topology by surface micropatterning technology. Here, we report a long-term cultivation of primary hippocampal neurons on microelectrode arrays using soft-lithography. Ordered hippocampal neuronal networks were formed by seeding neurons in agarose-microwells and inducing neurite outgrowth through microgrooves. Unlike previous approaches, our technique allowed us to design networks with various microwells on microelectrode arrays with high repeatability. These hippocampal network chips were cultivated for 30 days with excellent pattern fidelity, and neural spikes were successfully measured. We also found that spontaneous activity of the networks could be enhanced by acute disinhibition of inhibitory synapses. The proposed patterning method for neuronal network chips will be a potentially powerful tool for cell-based drug-screening applications. Neuron patterning (dpeaa)DE-He213 Microelectrode arrays (dpeaa)DE-He213 Surface patterning (dpeaa)DE-He213 Micromolding in capillaries (dpeaa)DE-He213 Long-term culture (dpeaa)DE-He213 Lim, Jisoon aut Nam, Yoonkey aut Enthalten in BioChip journal Seoul : Soc., 2007 12(2018), 3 vom: 10. Juli, Seite 193-201 (DE-627)608497258 (DE-600)2513796-7 2092-7843 nnns volume:12 year:2018 number:3 day:10 month:07 pages:193-201 https://dx.doi.org/10.1007/s13206-018-2308-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 12 2018 3 10 07 193-201
spelling	10.1007/s13206-018-2308-y doi (DE-627)SPR030871042 (SPR)s13206-018-2308-y-e DE-627 ger DE-627 rakwb eng Joo, Sunghoon verfasserin aut Design and Fabrication of Miniaturized Neuronal Circuits on Microelectrode Arrays Using Agarose Hydrogel Micro-molding Technique 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Korean BioChip Society and Springer-Verlag GmbH Germany, part of Springer Nature 2018 Abstract Dissociated neuronal cultures combined with planar-type microelectrode arrays (MEAs) have been used as a promising read-out platform for the application of cell-based biosensors. There are increasing interests in engineering neuronal cultures to form the desired network topology by surface micropatterning technology. Here, we report a long-term cultivation of primary hippocampal neurons on microelectrode arrays using soft-lithography. Ordered hippocampal neuronal networks were formed by seeding neurons in agarose-microwells and inducing neurite outgrowth through microgrooves. Unlike previous approaches, our technique allowed us to design networks with various microwells on microelectrode arrays with high repeatability. These hippocampal network chips were cultivated for 30 days with excellent pattern fidelity, and neural spikes were successfully measured. We also found that spontaneous activity of the networks could be enhanced by acute disinhibition of inhibitory synapses. The proposed patterning method for neuronal network chips will be a potentially powerful tool for cell-based drug-screening applications. Neuron patterning (dpeaa)DE-He213 Microelectrode arrays (dpeaa)DE-He213 Surface patterning (dpeaa)DE-He213 Micromolding in capillaries (dpeaa)DE-He213 Long-term culture (dpeaa)DE-He213 Lim, Jisoon aut Nam, Yoonkey aut Enthalten in BioChip journal Seoul : Soc., 2007 12(2018), 3 vom: 10. Juli, Seite 193-201 (DE-627)608497258 (DE-600)2513796-7 2092-7843 nnns volume:12 year:2018 number:3 day:10 month:07 pages:193-201 https://dx.doi.org/10.1007/s13206-018-2308-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 12 2018 3 10 07 193-201
allfields_unstemmed	10.1007/s13206-018-2308-y doi (DE-627)SPR030871042 (SPR)s13206-018-2308-y-e DE-627 ger DE-627 rakwb eng Joo, Sunghoon verfasserin aut Design and Fabrication of Miniaturized Neuronal Circuits on Microelectrode Arrays Using Agarose Hydrogel Micro-molding Technique 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Korean BioChip Society and Springer-Verlag GmbH Germany, part of Springer Nature 2018 Abstract Dissociated neuronal cultures combined with planar-type microelectrode arrays (MEAs) have been used as a promising read-out platform for the application of cell-based biosensors. There are increasing interests in engineering neuronal cultures to form the desired network topology by surface micropatterning technology. Here, we report a long-term cultivation of primary hippocampal neurons on microelectrode arrays using soft-lithography. Ordered hippocampal neuronal networks were formed by seeding neurons in agarose-microwells and inducing neurite outgrowth through microgrooves. Unlike previous approaches, our technique allowed us to design networks with various microwells on microelectrode arrays with high repeatability. These hippocampal network chips were cultivated for 30 days with excellent pattern fidelity, and neural spikes were successfully measured. We also found that spontaneous activity of the networks could be enhanced by acute disinhibition of inhibitory synapses. The proposed patterning method for neuronal network chips will be a potentially powerful tool for cell-based drug-screening applications. Neuron patterning (dpeaa)DE-He213 Microelectrode arrays (dpeaa)DE-He213 Surface patterning (dpeaa)DE-He213 Micromolding in capillaries (dpeaa)DE-He213 Long-term culture (dpeaa)DE-He213 Lim, Jisoon aut Nam, Yoonkey aut Enthalten in BioChip journal Seoul : Soc., 2007 12(2018), 3 vom: 10. Juli, Seite 193-201 (DE-627)608497258 (DE-600)2513796-7 2092-7843 nnns volume:12 year:2018 number:3 day:10 month:07 pages:193-201 https://dx.doi.org/10.1007/s13206-018-2308-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 12 2018 3 10 07 193-201
allfieldsGer	10.1007/s13206-018-2308-y doi (DE-627)SPR030871042 (SPR)s13206-018-2308-y-e DE-627 ger DE-627 rakwb eng Joo, Sunghoon verfasserin aut Design and Fabrication of Miniaturized Neuronal Circuits on Microelectrode Arrays Using Agarose Hydrogel Micro-molding Technique 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Korean BioChip Society and Springer-Verlag GmbH Germany, part of Springer Nature 2018 Abstract Dissociated neuronal cultures combined with planar-type microelectrode arrays (MEAs) have been used as a promising read-out platform for the application of cell-based biosensors. There are increasing interests in engineering neuronal cultures to form the desired network topology by surface micropatterning technology. Here, we report a long-term cultivation of primary hippocampal neurons on microelectrode arrays using soft-lithography. Ordered hippocampal neuronal networks were formed by seeding neurons in agarose-microwells and inducing neurite outgrowth through microgrooves. Unlike previous approaches, our technique allowed us to design networks with various microwells on microelectrode arrays with high repeatability. These hippocampal network chips were cultivated for 30 days with excellent pattern fidelity, and neural spikes were successfully measured. We also found that spontaneous activity of the networks could be enhanced by acute disinhibition of inhibitory synapses. The proposed patterning method for neuronal network chips will be a potentially powerful tool for cell-based drug-screening applications. Neuron patterning (dpeaa)DE-He213 Microelectrode arrays (dpeaa)DE-He213 Surface patterning (dpeaa)DE-He213 Micromolding in capillaries (dpeaa)DE-He213 Long-term culture (dpeaa)DE-He213 Lim, Jisoon aut Nam, Yoonkey aut Enthalten in BioChip journal Seoul : Soc., 2007 12(2018), 3 vom: 10. Juli, Seite 193-201 (DE-627)608497258 (DE-600)2513796-7 2092-7843 nnns volume:12 year:2018 number:3 day:10 month:07 pages:193-201 https://dx.doi.org/10.1007/s13206-018-2308-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 12 2018 3 10 07 193-201
allfieldsSound	10.1007/s13206-018-2308-y doi (DE-627)SPR030871042 (SPR)s13206-018-2308-y-e DE-627 ger DE-627 rakwb eng Joo, Sunghoon verfasserin aut Design and Fabrication of Miniaturized Neuronal Circuits on Microelectrode Arrays Using Agarose Hydrogel Micro-molding Technique 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Korean BioChip Society and Springer-Verlag GmbH Germany, part of Springer Nature 2018 Abstract Dissociated neuronal cultures combined with planar-type microelectrode arrays (MEAs) have been used as a promising read-out platform for the application of cell-based biosensors. There are increasing interests in engineering neuronal cultures to form the desired network topology by surface micropatterning technology. Here, we report a long-term cultivation of primary hippocampal neurons on microelectrode arrays using soft-lithography. Ordered hippocampal neuronal networks were formed by seeding neurons in agarose-microwells and inducing neurite outgrowth through microgrooves. Unlike previous approaches, our technique allowed us to design networks with various microwells on microelectrode arrays with high repeatability. These hippocampal network chips were cultivated for 30 days with excellent pattern fidelity, and neural spikes were successfully measured. We also found that spontaneous activity of the networks could be enhanced by acute disinhibition of inhibitory synapses. The proposed patterning method for neuronal network chips will be a potentially powerful tool for cell-based drug-screening applications. Neuron patterning (dpeaa)DE-He213 Microelectrode arrays (dpeaa)DE-He213 Surface patterning (dpeaa)DE-He213 Micromolding in capillaries (dpeaa)DE-He213 Long-term culture (dpeaa)DE-He213 Lim, Jisoon aut Nam, Yoonkey aut Enthalten in BioChip journal Seoul : Soc., 2007 12(2018), 3 vom: 10. Juli, Seite 193-201 (DE-627)608497258 (DE-600)2513796-7 2092-7843 nnns volume:12 year:2018 number:3 day:10 month:07 pages:193-201 https://dx.doi.org/10.1007/s13206-018-2308-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 12 2018 3 10 07 193-201
language	English
source	Enthalten in BioChip journal 12(2018), 3 vom: 10. Juli, Seite 193-201 volume:12 year:2018 number:3 day:10 month:07 pages:193-201
sourceStr	Enthalten in BioChip journal 12(2018), 3 vom: 10. Juli, Seite 193-201 volume:12 year:2018 number:3 day:10 month:07 pages:193-201
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Neuron patterning Microelectrode arrays Surface patterning Micromolding in capillaries Long-term culture
isfreeaccess_bool	false
container_title	BioChip journal
authorswithroles_txt_mv	Joo, Sunghoon @@aut@@ Lim, Jisoon @@aut@@ Nam, Yoonkey @@aut@@
publishDateDaySort_date	2018-07-10T00:00:00Z
hierarchy_top_id	608497258
id	SPR030871042
language_de	englisch
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author	Joo, Sunghoon
spellingShingle	Joo, Sunghoon misc Neuron patterning misc Microelectrode arrays misc Surface patterning misc Micromolding in capillaries misc Long-term culture Design and Fabrication of Miniaturized Neuronal Circuits on Microelectrode Arrays Using Agarose Hydrogel Micro-molding Technique
authorStr	Joo, Sunghoon
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topic_title	Design and Fabrication of Miniaturized Neuronal Circuits on Microelectrode Arrays Using Agarose Hydrogel Micro-molding Technique Neuron patterning (dpeaa)DE-He213 Microelectrode arrays (dpeaa)DE-He213 Surface patterning (dpeaa)DE-He213 Micromolding in capillaries (dpeaa)DE-He213 Long-term culture (dpeaa)DE-He213
topic	misc Neuron patterning misc Microelectrode arrays misc Surface patterning misc Micromolding in capillaries misc Long-term culture
topic_unstemmed	misc Neuron patterning misc Microelectrode arrays misc Surface patterning misc Micromolding in capillaries misc Long-term culture
topic_browse	misc Neuron patterning misc Microelectrode arrays misc Surface patterning misc Micromolding in capillaries misc Long-term culture
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title	Design and Fabrication of Miniaturized Neuronal Circuits on Microelectrode Arrays Using Agarose Hydrogel Micro-molding Technique
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title_full	Design and Fabrication of Miniaturized Neuronal Circuits on Microelectrode Arrays Using Agarose Hydrogel Micro-molding Technique
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author_browse	Joo, Sunghoon Lim, Jisoon Nam, Yoonkey
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doi_str_mv	10.1007/s13206-018-2308-y
title_sort	design and fabrication of miniaturized neuronal circuits on microelectrode arrays using agarose hydrogel micro-molding technique
title_auth	Design and Fabrication of Miniaturized Neuronal Circuits on Microelectrode Arrays Using Agarose Hydrogel Micro-molding Technique
abstract	Abstract Dissociated neuronal cultures combined with planar-type microelectrode arrays (MEAs) have been used as a promising read-out platform for the application of cell-based biosensors. There are increasing interests in engineering neuronal cultures to form the desired network topology by surface micropatterning technology. Here, we report a long-term cultivation of primary hippocampal neurons on microelectrode arrays using soft-lithography. Ordered hippocampal neuronal networks were formed by seeding neurons in agarose-microwells and inducing neurite outgrowth through microgrooves. Unlike previous approaches, our technique allowed us to design networks with various microwells on microelectrode arrays with high repeatability. These hippocampal network chips were cultivated for 30 days with excellent pattern fidelity, and neural spikes were successfully measured. We also found that spontaneous activity of the networks could be enhanced by acute disinhibition of inhibitory synapses. The proposed patterning method for neuronal network chips will be a potentially powerful tool for cell-based drug-screening applications. © The Korean BioChip Society and Springer-Verlag GmbH Germany, part of Springer Nature 2018
abstractGer	Abstract Dissociated neuronal cultures combined with planar-type microelectrode arrays (MEAs) have been used as a promising read-out platform for the application of cell-based biosensors. There are increasing interests in engineering neuronal cultures to form the desired network topology by surface micropatterning technology. Here, we report a long-term cultivation of primary hippocampal neurons on microelectrode arrays using soft-lithography. Ordered hippocampal neuronal networks were formed by seeding neurons in agarose-microwells and inducing neurite outgrowth through microgrooves. Unlike previous approaches, our technique allowed us to design networks with various microwells on microelectrode arrays with high repeatability. These hippocampal network chips were cultivated for 30 days with excellent pattern fidelity, and neural spikes were successfully measured. We also found that spontaneous activity of the networks could be enhanced by acute disinhibition of inhibitory synapses. The proposed patterning method for neuronal network chips will be a potentially powerful tool for cell-based drug-screening applications. © The Korean BioChip Society and Springer-Verlag GmbH Germany, part of Springer Nature 2018
abstract_unstemmed	Abstract Dissociated neuronal cultures combined with planar-type microelectrode arrays (MEAs) have been used as a promising read-out platform for the application of cell-based biosensors. There are increasing interests in engineering neuronal cultures to form the desired network topology by surface micropatterning technology. Here, we report a long-term cultivation of primary hippocampal neurons on microelectrode arrays using soft-lithography. Ordered hippocampal neuronal networks were formed by seeding neurons in agarose-microwells and inducing neurite outgrowth through microgrooves. Unlike previous approaches, our technique allowed us to design networks with various microwells on microelectrode arrays with high repeatability. These hippocampal network chips were cultivated for 30 days with excellent pattern fidelity, and neural spikes were successfully measured. We also found that spontaneous activity of the networks could be enhanced by acute disinhibition of inhibitory synapses. The proposed patterning method for neuronal network chips will be a potentially powerful tool for cell-based drug-screening applications. © The Korean BioChip Society and Springer-Verlag GmbH Germany, part of Springer Nature 2018
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container_issue	3
title_short	Design and Fabrication of Miniaturized Neuronal Circuits on Microelectrode Arrays Using Agarose Hydrogel Micro-molding Technique
url	https://dx.doi.org/10.1007/s13206-018-2308-y
remote_bool	true
author2	Lim, Jisoon Nam, Yoonkey
author2Str	Lim, Jisoon Nam, Yoonkey
ppnlink	608497258
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doi_str	10.1007/s13206-018-2308-y
up_date	2024-07-03T20:37:35.046Z
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Design and Fabrication of Miniaturized Neuronal Circuits on Microelectrode Arrays Using Agarose Hydrogel Micro-molding Technique

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