The use of force-volume microscopy to examine bacterial attachment to titanium surfaces

Abstract Force-volume microscopy (FVM) was used to study the interfacial and adhesive forces affecting primary bacterial attachment to surfaces. Forces were measured for titanium surfaces immersed either in cation-enriched (CE) solutions of yeast extract amended with phosphate buffer or in control s...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Na, Chongzheng [verfasserIn] McNamara, Christopher J. Konkol, Nick R. Bearce, Kristen A. Mitchell, Ralph Martin, Scot T.

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2010

Schlagwörter:	Force-volume microscopy Atomic force microscopy Interfacial forces Surface adhesion

Anmerkung:	© Springer-Verlag and the University of Milan 2010

Übergeordnetes Werk:	Enthalten in: Annals of microbiology - Berlin : Springer, 1998, 60(2010), 3 vom: 01. Juli, Seite 495-502
Übergeordnetes Werk:	volume:60 ; year:2010 ; number:3 ; day:01 ; month:07 ; pages:495-502

Links:	Volltext

DOI / URN:	10.1007/s13213-010-0078-4

Katalog-ID:	SPR030875099

Internformat


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520			\|a Abstract Force-volume microscopy (FVM) was used to study the interfacial and adhesive forces affecting primary bacterial attachment to surfaces. Forces were measured for titanium surfaces immersed either in cation-enriched (CE) solutions of yeast extract amended with phosphate buffer or in control solutions lacking the cation enrichment. The FVM measurements demonstrated that regions of elevated interfacial repulsion covered 72(±2)% of the surfaces immersed in CE solutions, compared to 26(±2)% for immersion in control solutions. Parallel collection of scanning electron micrographs demonstrated that surface densities of attached Pseudomonas aeruginosa were approximately 0.62(±1.3) × $ 10^{6} $ cells $ cm^{−2} $ compared to 8.7(±0.8) × $ 10^{6} $ cells $ cm^{−2} $ for surfaces immersed in the CE and control solutions, respectively. Interfacial repulsion indicated by FVM measurements therefore served as a predictor of bacterial attachment. Another factor influencing bacterial attachment was the adhesion force. FVM measurements indicated that the upper fifth percentile of surface adhesion was 1784(±40) pN for surfaces immersed in the CE solution compared to 2284(±40) pN for the control solutions. The more extensive regions of elevated interfacial repulsion as well as of decreased surface adhesion provide an explanation for the lower density of attached cells observed for the surfaces immersed in the CE compared to the control solutions. The conclusion is that FVM is a sensitive and informative technique that can be used to measure and explain interactions between microorganisms and surfaces.
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author_variant	c n cn c j m cj cjm n r k nr nrk k a b ka kab r m rm s t m st stm
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allfields	10.1007/s13213-010-0078-4 doi (DE-627)SPR030875099 (SPR)s13213-010-0078-4-e DE-627 ger DE-627 rakwb eng Na, Chongzheng verfasserin aut The use of force-volume microscopy to examine bacterial attachment to titanium surfaces 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag and the University of Milan 2010 Abstract Force-volume microscopy (FVM) was used to study the interfacial and adhesive forces affecting primary bacterial attachment to surfaces. Forces were measured for titanium surfaces immersed either in cation-enriched (CE) solutions of yeast extract amended with phosphate buffer or in control solutions lacking the cation enrichment. The FVM measurements demonstrated that regions of elevated interfacial repulsion covered 72(±2)% of the surfaces immersed in CE solutions, compared to 26(±2)% for immersion in control solutions. Parallel collection of scanning electron micrographs demonstrated that surface densities of attached Pseudomonas aeruginosa were approximately 0.62(±1.3) × $ 10^{6} $ cells $ cm^{−2} $ compared to 8.7(±0.8) × $ 10^{6} $ cells $ cm^{−2} $ for surfaces immersed in the CE and control solutions, respectively. Interfacial repulsion indicated by FVM measurements therefore served as a predictor of bacterial attachment. Another factor influencing bacterial attachment was the adhesion force. FVM measurements indicated that the upper fifth percentile of surface adhesion was 1784(±40) pN for surfaces immersed in the CE solution compared to 2284(±40) pN for the control solutions. The more extensive regions of elevated interfacial repulsion as well as of decreased surface adhesion provide an explanation for the lower density of attached cells observed for the surfaces immersed in the CE compared to the control solutions. The conclusion is that FVM is a sensitive and informative technique that can be used to measure and explain interactions between microorganisms and surfaces. Force-volume microscopy (dpeaa)DE-He213 Atomic force microscopy (dpeaa)DE-He213 Interfacial forces (dpeaa)DE-He213 Surface adhesion (dpeaa)DE-He213 McNamara, Christopher J. aut Konkol, Nick R. aut Bearce, Kristen A. aut Mitchell, Ralph aut Martin, Scot T. aut Enthalten in Annals of microbiology Berlin : Springer, 1998 60(2010), 3 vom: 01. Juli, Seite 495-502 (DE-627)385615434 (DE-600)2143009-3 1869-2044 nnns volume:60 year:2010 number:3 day:01 month:07 pages:495-502 https://dx.doi.org/10.1007/s13213-010-0078-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_22 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_63 GBV_ILN_70 GBV_ILN_95 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_187 GBV_ILN_285 GBV_ILN_370 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4313 GBV_ILN_4328 GBV_ILN_4333 AR 60 2010 3 01 07 495-502
spelling	10.1007/s13213-010-0078-4 doi (DE-627)SPR030875099 (SPR)s13213-010-0078-4-e DE-627 ger DE-627 rakwb eng Na, Chongzheng verfasserin aut The use of force-volume microscopy to examine bacterial attachment to titanium surfaces 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag and the University of Milan 2010 Abstract Force-volume microscopy (FVM) was used to study the interfacial and adhesive forces affecting primary bacterial attachment to surfaces. Forces were measured for titanium surfaces immersed either in cation-enriched (CE) solutions of yeast extract amended with phosphate buffer or in control solutions lacking the cation enrichment. The FVM measurements demonstrated that regions of elevated interfacial repulsion covered 72(±2)% of the surfaces immersed in CE solutions, compared to 26(±2)% for immersion in control solutions. Parallel collection of scanning electron micrographs demonstrated that surface densities of attached Pseudomonas aeruginosa were approximately 0.62(±1.3) × $ 10^{6} $ cells $ cm^{−2} $ compared to 8.7(±0.8) × $ 10^{6} $ cells $ cm^{−2} $ for surfaces immersed in the CE and control solutions, respectively. Interfacial repulsion indicated by FVM measurements therefore served as a predictor of bacterial attachment. Another factor influencing bacterial attachment was the adhesion force. FVM measurements indicated that the upper fifth percentile of surface adhesion was 1784(±40) pN for surfaces immersed in the CE solution compared to 2284(±40) pN for the control solutions. The more extensive regions of elevated interfacial repulsion as well as of decreased surface adhesion provide an explanation for the lower density of attached cells observed for the surfaces immersed in the CE compared to the control solutions. The conclusion is that FVM is a sensitive and informative technique that can be used to measure and explain interactions between microorganisms and surfaces. Force-volume microscopy (dpeaa)DE-He213 Atomic force microscopy (dpeaa)DE-He213 Interfacial forces (dpeaa)DE-He213 Surface adhesion (dpeaa)DE-He213 McNamara, Christopher J. aut Konkol, Nick R. aut Bearce, Kristen A. aut Mitchell, Ralph aut Martin, Scot T. aut Enthalten in Annals of microbiology Berlin : Springer, 1998 60(2010), 3 vom: 01. Juli, Seite 495-502 (DE-627)385615434 (DE-600)2143009-3 1869-2044 nnns volume:60 year:2010 number:3 day:01 month:07 pages:495-502 https://dx.doi.org/10.1007/s13213-010-0078-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_22 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_63 GBV_ILN_70 GBV_ILN_95 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_187 GBV_ILN_285 GBV_ILN_370 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4313 GBV_ILN_4328 GBV_ILN_4333 AR 60 2010 3 01 07 495-502
allfields_unstemmed	10.1007/s13213-010-0078-4 doi (DE-627)SPR030875099 (SPR)s13213-010-0078-4-e DE-627 ger DE-627 rakwb eng Na, Chongzheng verfasserin aut The use of force-volume microscopy to examine bacterial attachment to titanium surfaces 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag and the University of Milan 2010 Abstract Force-volume microscopy (FVM) was used to study the interfacial and adhesive forces affecting primary bacterial attachment to surfaces. Forces were measured for titanium surfaces immersed either in cation-enriched (CE) solutions of yeast extract amended with phosphate buffer or in control solutions lacking the cation enrichment. The FVM measurements demonstrated that regions of elevated interfacial repulsion covered 72(±2)% of the surfaces immersed in CE solutions, compared to 26(±2)% for immersion in control solutions. Parallel collection of scanning electron micrographs demonstrated that surface densities of attached Pseudomonas aeruginosa were approximately 0.62(±1.3) × $ 10^{6} $ cells $ cm^{−2} $ compared to 8.7(±0.8) × $ 10^{6} $ cells $ cm^{−2} $ for surfaces immersed in the CE and control solutions, respectively. Interfacial repulsion indicated by FVM measurements therefore served as a predictor of bacterial attachment. Another factor influencing bacterial attachment was the adhesion force. FVM measurements indicated that the upper fifth percentile of surface adhesion was 1784(±40) pN for surfaces immersed in the CE solution compared to 2284(±40) pN for the control solutions. The more extensive regions of elevated interfacial repulsion as well as of decreased surface adhesion provide an explanation for the lower density of attached cells observed for the surfaces immersed in the CE compared to the control solutions. The conclusion is that FVM is a sensitive and informative technique that can be used to measure and explain interactions between microorganisms and surfaces. Force-volume microscopy (dpeaa)DE-He213 Atomic force microscopy (dpeaa)DE-He213 Interfacial forces (dpeaa)DE-He213 Surface adhesion (dpeaa)DE-He213 McNamara, Christopher J. aut Konkol, Nick R. aut Bearce, Kristen A. aut Mitchell, Ralph aut Martin, Scot T. aut Enthalten in Annals of microbiology Berlin : Springer, 1998 60(2010), 3 vom: 01. Juli, Seite 495-502 (DE-627)385615434 (DE-600)2143009-3 1869-2044 nnns volume:60 year:2010 number:3 day:01 month:07 pages:495-502 https://dx.doi.org/10.1007/s13213-010-0078-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_22 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_63 GBV_ILN_70 GBV_ILN_95 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_187 GBV_ILN_285 GBV_ILN_370 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4313 GBV_ILN_4328 GBV_ILN_4333 AR 60 2010 3 01 07 495-502
allfieldsGer	10.1007/s13213-010-0078-4 doi (DE-627)SPR030875099 (SPR)s13213-010-0078-4-e DE-627 ger DE-627 rakwb eng Na, Chongzheng verfasserin aut The use of force-volume microscopy to examine bacterial attachment to titanium surfaces 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag and the University of Milan 2010 Abstract Force-volume microscopy (FVM) was used to study the interfacial and adhesive forces affecting primary bacterial attachment to surfaces. Forces were measured for titanium surfaces immersed either in cation-enriched (CE) solutions of yeast extract amended with phosphate buffer or in control solutions lacking the cation enrichment. The FVM measurements demonstrated that regions of elevated interfacial repulsion covered 72(±2)% of the surfaces immersed in CE solutions, compared to 26(±2)% for immersion in control solutions. Parallel collection of scanning electron micrographs demonstrated that surface densities of attached Pseudomonas aeruginosa were approximately 0.62(±1.3) × $ 10^{6} $ cells $ cm^{−2} $ compared to 8.7(±0.8) × $ 10^{6} $ cells $ cm^{−2} $ for surfaces immersed in the CE and control solutions, respectively. Interfacial repulsion indicated by FVM measurements therefore served as a predictor of bacterial attachment. Another factor influencing bacterial attachment was the adhesion force. FVM measurements indicated that the upper fifth percentile of surface adhesion was 1784(±40) pN for surfaces immersed in the CE solution compared to 2284(±40) pN for the control solutions. The more extensive regions of elevated interfacial repulsion as well as of decreased surface adhesion provide an explanation for the lower density of attached cells observed for the surfaces immersed in the CE compared to the control solutions. The conclusion is that FVM is a sensitive and informative technique that can be used to measure and explain interactions between microorganisms and surfaces. Force-volume microscopy (dpeaa)DE-He213 Atomic force microscopy (dpeaa)DE-He213 Interfacial forces (dpeaa)DE-He213 Surface adhesion (dpeaa)DE-He213 McNamara, Christopher J. aut Konkol, Nick R. aut Bearce, Kristen A. aut Mitchell, Ralph aut Martin, Scot T. aut Enthalten in Annals of microbiology Berlin : Springer, 1998 60(2010), 3 vom: 01. Juli, Seite 495-502 (DE-627)385615434 (DE-600)2143009-3 1869-2044 nnns volume:60 year:2010 number:3 day:01 month:07 pages:495-502 https://dx.doi.org/10.1007/s13213-010-0078-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_22 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_63 GBV_ILN_70 GBV_ILN_95 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_187 GBV_ILN_285 GBV_ILN_370 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4313 GBV_ILN_4328 GBV_ILN_4333 AR 60 2010 3 01 07 495-502
allfieldsSound	10.1007/s13213-010-0078-4 doi (DE-627)SPR030875099 (SPR)s13213-010-0078-4-e DE-627 ger DE-627 rakwb eng Na, Chongzheng verfasserin aut The use of force-volume microscopy to examine bacterial attachment to titanium surfaces 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag and the University of Milan 2010 Abstract Force-volume microscopy (FVM) was used to study the interfacial and adhesive forces affecting primary bacterial attachment to surfaces. Forces were measured for titanium surfaces immersed either in cation-enriched (CE) solutions of yeast extract amended with phosphate buffer or in control solutions lacking the cation enrichment. The FVM measurements demonstrated that regions of elevated interfacial repulsion covered 72(±2)% of the surfaces immersed in CE solutions, compared to 26(±2)% for immersion in control solutions. Parallel collection of scanning electron micrographs demonstrated that surface densities of attached Pseudomonas aeruginosa were approximately 0.62(±1.3) × $ 10^{6} $ cells $ cm^{−2} $ compared to 8.7(±0.8) × $ 10^{6} $ cells $ cm^{−2} $ for surfaces immersed in the CE and control solutions, respectively. Interfacial repulsion indicated by FVM measurements therefore served as a predictor of bacterial attachment. Another factor influencing bacterial attachment was the adhesion force. FVM measurements indicated that the upper fifth percentile of surface adhesion was 1784(±40) pN for surfaces immersed in the CE solution compared to 2284(±40) pN for the control solutions. The more extensive regions of elevated interfacial repulsion as well as of decreased surface adhesion provide an explanation for the lower density of attached cells observed for the surfaces immersed in the CE compared to the control solutions. The conclusion is that FVM is a sensitive and informative technique that can be used to measure and explain interactions between microorganisms and surfaces. Force-volume microscopy (dpeaa)DE-He213 Atomic force microscopy (dpeaa)DE-He213 Interfacial forces (dpeaa)DE-He213 Surface adhesion (dpeaa)DE-He213 McNamara, Christopher J. aut Konkol, Nick R. aut Bearce, Kristen A. aut Mitchell, Ralph aut Martin, Scot T. aut Enthalten in Annals of microbiology Berlin : Springer, 1998 60(2010), 3 vom: 01. Juli, Seite 495-502 (DE-627)385615434 (DE-600)2143009-3 1869-2044 nnns volume:60 year:2010 number:3 day:01 month:07 pages:495-502 https://dx.doi.org/10.1007/s13213-010-0078-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_22 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_63 GBV_ILN_70 GBV_ILN_95 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_187 GBV_ILN_285 GBV_ILN_370 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4313 GBV_ILN_4328 GBV_ILN_4333 AR 60 2010 3 01 07 495-502
language	English
source	Enthalten in Annals of microbiology 60(2010), 3 vom: 01. Juli, Seite 495-502 volume:60 year:2010 number:3 day:01 month:07 pages:495-502
sourceStr	Enthalten in Annals of microbiology 60(2010), 3 vom: 01. Juli, Seite 495-502 volume:60 year:2010 number:3 day:01 month:07 pages:495-502
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Force-volume microscopy Atomic force microscopy Interfacial forces Surface adhesion
isfreeaccess_bool	false
container_title	Annals of microbiology
authorswithroles_txt_mv	Na, Chongzheng @@aut@@ McNamara, Christopher J. @@aut@@ Konkol, Nick R. @@aut@@ Bearce, Kristen A. @@aut@@ Mitchell, Ralph @@aut@@ Martin, Scot T. @@aut@@
publishDateDaySort_date	2010-07-01T00:00:00Z
hierarchy_top_id	385615434
id	SPR030875099
language_de	englisch
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author	Na, Chongzheng
spellingShingle	Na, Chongzheng misc Force-volume microscopy misc Atomic force microscopy misc Interfacial forces misc Surface adhesion The use of force-volume microscopy to examine bacterial attachment to titanium surfaces
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topic_title	The use of force-volume microscopy to examine bacterial attachment to titanium surfaces Force-volume microscopy (dpeaa)DE-He213 Atomic force microscopy (dpeaa)DE-He213 Interfacial forces (dpeaa)DE-He213 Surface adhesion (dpeaa)DE-He213
topic	misc Force-volume microscopy misc Atomic force microscopy misc Interfacial forces misc Surface adhesion
topic_unstemmed	misc Force-volume microscopy misc Atomic force microscopy misc Interfacial forces misc Surface adhesion
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title	The use of force-volume microscopy to examine bacterial attachment to titanium surfaces
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title_full	The use of force-volume microscopy to examine bacterial attachment to titanium surfaces
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author_browse	Na, Chongzheng McNamara, Christopher J. Konkol, Nick R. Bearce, Kristen A. Mitchell, Ralph Martin, Scot T.
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title_sort	use of force-volume microscopy to examine bacterial attachment to titanium surfaces
title_auth	The use of force-volume microscopy to examine bacterial attachment to titanium surfaces
abstract	Abstract Force-volume microscopy (FVM) was used to study the interfacial and adhesive forces affecting primary bacterial attachment to surfaces. Forces were measured for titanium surfaces immersed either in cation-enriched (CE) solutions of yeast extract amended with phosphate buffer or in control solutions lacking the cation enrichment. The FVM measurements demonstrated that regions of elevated interfacial repulsion covered 72(±2)% of the surfaces immersed in CE solutions, compared to 26(±2)% for immersion in control solutions. Parallel collection of scanning electron micrographs demonstrated that surface densities of attached Pseudomonas aeruginosa were approximately 0.62(±1.3) × $ 10^{6} $ cells $ cm^{−2} $ compared to 8.7(±0.8) × $ 10^{6} $ cells $ cm^{−2} $ for surfaces immersed in the CE and control solutions, respectively. Interfacial repulsion indicated by FVM measurements therefore served as a predictor of bacterial attachment. Another factor influencing bacterial attachment was the adhesion force. FVM measurements indicated that the upper fifth percentile of surface adhesion was 1784(±40) pN for surfaces immersed in the CE solution compared to 2284(±40) pN for the control solutions. The more extensive regions of elevated interfacial repulsion as well as of decreased surface adhesion provide an explanation for the lower density of attached cells observed for the surfaces immersed in the CE compared to the control solutions. The conclusion is that FVM is a sensitive and informative technique that can be used to measure and explain interactions between microorganisms and surfaces. © Springer-Verlag and the University of Milan 2010
abstractGer	Abstract Force-volume microscopy (FVM) was used to study the interfacial and adhesive forces affecting primary bacterial attachment to surfaces. Forces were measured for titanium surfaces immersed either in cation-enriched (CE) solutions of yeast extract amended with phosphate buffer or in control solutions lacking the cation enrichment. The FVM measurements demonstrated that regions of elevated interfacial repulsion covered 72(±2)% of the surfaces immersed in CE solutions, compared to 26(±2)% for immersion in control solutions. Parallel collection of scanning electron micrographs demonstrated that surface densities of attached Pseudomonas aeruginosa were approximately 0.62(±1.3) × $ 10^{6} $ cells $ cm^{−2} $ compared to 8.7(±0.8) × $ 10^{6} $ cells $ cm^{−2} $ for surfaces immersed in the CE and control solutions, respectively. Interfacial repulsion indicated by FVM measurements therefore served as a predictor of bacterial attachment. Another factor influencing bacterial attachment was the adhesion force. FVM measurements indicated that the upper fifth percentile of surface adhesion was 1784(±40) pN for surfaces immersed in the CE solution compared to 2284(±40) pN for the control solutions. The more extensive regions of elevated interfacial repulsion as well as of decreased surface adhesion provide an explanation for the lower density of attached cells observed for the surfaces immersed in the CE compared to the control solutions. The conclusion is that FVM is a sensitive and informative technique that can be used to measure and explain interactions between microorganisms and surfaces. © Springer-Verlag and the University of Milan 2010
abstract_unstemmed	Abstract Force-volume microscopy (FVM) was used to study the interfacial and adhesive forces affecting primary bacterial attachment to surfaces. Forces were measured for titanium surfaces immersed either in cation-enriched (CE) solutions of yeast extract amended with phosphate buffer or in control solutions lacking the cation enrichment. The FVM measurements demonstrated that regions of elevated interfacial repulsion covered 72(±2)% of the surfaces immersed in CE solutions, compared to 26(±2)% for immersion in control solutions. Parallel collection of scanning electron micrographs demonstrated that surface densities of attached Pseudomonas aeruginosa were approximately 0.62(±1.3) × $ 10^{6} $ cells $ cm^{−2} $ compared to 8.7(±0.8) × $ 10^{6} $ cells $ cm^{−2} $ for surfaces immersed in the CE and control solutions, respectively. Interfacial repulsion indicated by FVM measurements therefore served as a predictor of bacterial attachment. Another factor influencing bacterial attachment was the adhesion force. FVM measurements indicated that the upper fifth percentile of surface adhesion was 1784(±40) pN for surfaces immersed in the CE solution compared to 2284(±40) pN for the control solutions. The more extensive regions of elevated interfacial repulsion as well as of decreased surface adhesion provide an explanation for the lower density of attached cells observed for the surfaces immersed in the CE compared to the control solutions. The conclusion is that FVM is a sensitive and informative technique that can be used to measure and explain interactions between microorganisms and surfaces. © Springer-Verlag and the University of Milan 2010
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title_short	The use of force-volume microscopy to examine bacterial attachment to titanium surfaces
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Nicht das Richtige dabei?