Repeated batch ethanolic fermentation of very high gravity medium by immobilized Saccharomyces cerevisiae

Abstract The main objective of this study was to evaluate the effect of yeast immobilization on ethanolic fermentation of very high gravity (VHG) medium and to determine the concentrations of yeast storage carbohydrates like trehalose and glycogen during the process. Repeated batch ethanolic ferment...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Puligundla, Pradeep [verfasserIn] Poludasu, Rama Mohan Rai, Jithan Kumar Obulam, Vijaya Sarathi Reddy

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2011

Schlagwörter:	Immobilization Repeated batch fermentation Very high gravity medium

Anmerkung:	© Springer-Verlag and the University of Milan 2011

Übergeordnetes Werk:	Enthalten in: Annals of microbiology - Berlin : Springer, 1998, 61(2011), 4 vom: 05. Feb., Seite 863-869
Übergeordnetes Werk:	volume:61 ; year:2011 ; number:4 ; day:05 ; month:02 ; pages:863-869

Links:	Volltext

DOI / URN:	10.1007/s13213-011-0207-8

Katalog-ID:	SPR030876613

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520			\|a Abstract The main objective of this study was to evaluate the effect of yeast immobilization on ethanolic fermentation of very high gravity (VHG) medium and to determine the concentrations of yeast storage carbohydrates like trehalose and glycogen during the process. Repeated batch ethanolic fermentation of VHG medium was carried out using Saccharomyces cerevisiae immobilized separately within Ca-alginate and κ-carrageenan polymers. Immobilization yields (YI) were between 80 and 90% and ethanol yields ($ Y_{P/S} $) were more than 0.41 with both carriers. An average fermentation efficiency of nearly 70% was observed in 48-h fermentation batches. Compared to free cells, a reduction of more than 50% in the accumulated trehalose, and a two-fold increase in intracellular glycogen levels were observed in immobilized yeast cells at 24 and 48 h of fermentation, respectively, with both carriers. The increased viability (up to four-fold higher) upon 18% ethanol treatment for 2 h, and the sustained viability over four successive batches of immobilized cells showed the protective nature of the polymer carriers. The chemical nature of the carriers was not found to have any adverse effect on ethanol yields. Application of immobilized yeast in porous matrices may serve as a feasible and better technique for ethanol production, at both pilot and industrial scale.
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allfields	10.1007/s13213-011-0207-8 doi (DE-627)SPR030876613 (SPR)s13213-011-0207-8-e DE-627 ger DE-627 rakwb eng Puligundla, Pradeep verfasserin aut Repeated batch ethanolic fermentation of very high gravity medium by immobilized Saccharomyces cerevisiae 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag and the University of Milan 2011 Abstract The main objective of this study was to evaluate the effect of yeast immobilization on ethanolic fermentation of very high gravity (VHG) medium and to determine the concentrations of yeast storage carbohydrates like trehalose and glycogen during the process. Repeated batch ethanolic fermentation of VHG medium was carried out using Saccharomyces cerevisiae immobilized separately within Ca-alginate and κ-carrageenan polymers. Immobilization yields (YI) were between 80 and 90% and ethanol yields ($ Y_{P/S} $) were more than 0.41 with both carriers. An average fermentation efficiency of nearly 70% was observed in 48-h fermentation batches. Compared to free cells, a reduction of more than 50% in the accumulated trehalose, and a two-fold increase in intracellular glycogen levels were observed in immobilized yeast cells at 24 and 48 h of fermentation, respectively, with both carriers. The increased viability (up to four-fold higher) upon 18% ethanol treatment for 2 h, and the sustained viability over four successive batches of immobilized cells showed the protective nature of the polymer carriers. The chemical nature of the carriers was not found to have any adverse effect on ethanol yields. Application of immobilized yeast in porous matrices may serve as a feasible and better technique for ethanol production, at both pilot and industrial scale. Immobilization (dpeaa)DE-He213 Repeated batch fermentation (dpeaa)DE-He213 Very high gravity medium (dpeaa)DE-He213 Poludasu, Rama Mohan aut Rai, Jithan Kumar aut Obulam, Vijaya Sarathi Reddy aut Enthalten in Annals of microbiology Berlin : Springer, 1998 61(2011), 4 vom: 05. Feb., Seite 863-869 (DE-627)385615434 (DE-600)2143009-3 1869-2044 nnns volume:61 year:2011 number:4 day:05 month:02 pages:863-869 https://dx.doi.org/10.1007/s13213-011-0207-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_22 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_63 GBV_ILN_70 GBV_ILN_95 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_187 GBV_ILN_285 GBV_ILN_370 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4313 GBV_ILN_4328 GBV_ILN_4333 AR 61 2011 4 05 02 863-869
spelling	10.1007/s13213-011-0207-8 doi (DE-627)SPR030876613 (SPR)s13213-011-0207-8-e DE-627 ger DE-627 rakwb eng Puligundla, Pradeep verfasserin aut Repeated batch ethanolic fermentation of very high gravity medium by immobilized Saccharomyces cerevisiae 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag and the University of Milan 2011 Abstract The main objective of this study was to evaluate the effect of yeast immobilization on ethanolic fermentation of very high gravity (VHG) medium and to determine the concentrations of yeast storage carbohydrates like trehalose and glycogen during the process. Repeated batch ethanolic fermentation of VHG medium was carried out using Saccharomyces cerevisiae immobilized separately within Ca-alginate and κ-carrageenan polymers. Immobilization yields (YI) were between 80 and 90% and ethanol yields ($ Y_{P/S} $) were more than 0.41 with both carriers. An average fermentation efficiency of nearly 70% was observed in 48-h fermentation batches. Compared to free cells, a reduction of more than 50% in the accumulated trehalose, and a two-fold increase in intracellular glycogen levels were observed in immobilized yeast cells at 24 and 48 h of fermentation, respectively, with both carriers. The increased viability (up to four-fold higher) upon 18% ethanol treatment for 2 h, and the sustained viability over four successive batches of immobilized cells showed the protective nature of the polymer carriers. The chemical nature of the carriers was not found to have any adverse effect on ethanol yields. Application of immobilized yeast in porous matrices may serve as a feasible and better technique for ethanol production, at both pilot and industrial scale. Immobilization (dpeaa)DE-He213 Repeated batch fermentation (dpeaa)DE-He213 Very high gravity medium (dpeaa)DE-He213 Poludasu, Rama Mohan aut Rai, Jithan Kumar aut Obulam, Vijaya Sarathi Reddy aut Enthalten in Annals of microbiology Berlin : Springer, 1998 61(2011), 4 vom: 05. Feb., Seite 863-869 (DE-627)385615434 (DE-600)2143009-3 1869-2044 nnns volume:61 year:2011 number:4 day:05 month:02 pages:863-869 https://dx.doi.org/10.1007/s13213-011-0207-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_22 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_63 GBV_ILN_70 GBV_ILN_95 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_187 GBV_ILN_285 GBV_ILN_370 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4313 GBV_ILN_4328 GBV_ILN_4333 AR 61 2011 4 05 02 863-869
allfields_unstemmed	10.1007/s13213-011-0207-8 doi (DE-627)SPR030876613 (SPR)s13213-011-0207-8-e DE-627 ger DE-627 rakwb eng Puligundla, Pradeep verfasserin aut Repeated batch ethanolic fermentation of very high gravity medium by immobilized Saccharomyces cerevisiae 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag and the University of Milan 2011 Abstract The main objective of this study was to evaluate the effect of yeast immobilization on ethanolic fermentation of very high gravity (VHG) medium and to determine the concentrations of yeast storage carbohydrates like trehalose and glycogen during the process. Repeated batch ethanolic fermentation of VHG medium was carried out using Saccharomyces cerevisiae immobilized separately within Ca-alginate and κ-carrageenan polymers. Immobilization yields (YI) were between 80 and 90% and ethanol yields ($ Y_{P/S} $) were more than 0.41 with both carriers. An average fermentation efficiency of nearly 70% was observed in 48-h fermentation batches. Compared to free cells, a reduction of more than 50% in the accumulated trehalose, and a two-fold increase in intracellular glycogen levels were observed in immobilized yeast cells at 24 and 48 h of fermentation, respectively, with both carriers. The increased viability (up to four-fold higher) upon 18% ethanol treatment for 2 h, and the sustained viability over four successive batches of immobilized cells showed the protective nature of the polymer carriers. The chemical nature of the carriers was not found to have any adverse effect on ethanol yields. Application of immobilized yeast in porous matrices may serve as a feasible and better technique for ethanol production, at both pilot and industrial scale. Immobilization (dpeaa)DE-He213 Repeated batch fermentation (dpeaa)DE-He213 Very high gravity medium (dpeaa)DE-He213 Poludasu, Rama Mohan aut Rai, Jithan Kumar aut Obulam, Vijaya Sarathi Reddy aut Enthalten in Annals of microbiology Berlin : Springer, 1998 61(2011), 4 vom: 05. Feb., Seite 863-869 (DE-627)385615434 (DE-600)2143009-3 1869-2044 nnns volume:61 year:2011 number:4 day:05 month:02 pages:863-869 https://dx.doi.org/10.1007/s13213-011-0207-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_22 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_63 GBV_ILN_70 GBV_ILN_95 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_187 GBV_ILN_285 GBV_ILN_370 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4313 GBV_ILN_4328 GBV_ILN_4333 AR 61 2011 4 05 02 863-869
allfieldsGer	10.1007/s13213-011-0207-8 doi (DE-627)SPR030876613 (SPR)s13213-011-0207-8-e DE-627 ger DE-627 rakwb eng Puligundla, Pradeep verfasserin aut Repeated batch ethanolic fermentation of very high gravity medium by immobilized Saccharomyces cerevisiae 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag and the University of Milan 2011 Abstract The main objective of this study was to evaluate the effect of yeast immobilization on ethanolic fermentation of very high gravity (VHG) medium and to determine the concentrations of yeast storage carbohydrates like trehalose and glycogen during the process. Repeated batch ethanolic fermentation of VHG medium was carried out using Saccharomyces cerevisiae immobilized separately within Ca-alginate and κ-carrageenan polymers. Immobilization yields (YI) were between 80 and 90% and ethanol yields ($ Y_{P/S} $) were more than 0.41 with both carriers. An average fermentation efficiency of nearly 70% was observed in 48-h fermentation batches. Compared to free cells, a reduction of more than 50% in the accumulated trehalose, and a two-fold increase in intracellular glycogen levels were observed in immobilized yeast cells at 24 and 48 h of fermentation, respectively, with both carriers. The increased viability (up to four-fold higher) upon 18% ethanol treatment for 2 h, and the sustained viability over four successive batches of immobilized cells showed the protective nature of the polymer carriers. The chemical nature of the carriers was not found to have any adverse effect on ethanol yields. Application of immobilized yeast in porous matrices may serve as a feasible and better technique for ethanol production, at both pilot and industrial scale. Immobilization (dpeaa)DE-He213 Repeated batch fermentation (dpeaa)DE-He213 Very high gravity medium (dpeaa)DE-He213 Poludasu, Rama Mohan aut Rai, Jithan Kumar aut Obulam, Vijaya Sarathi Reddy aut Enthalten in Annals of microbiology Berlin : Springer, 1998 61(2011), 4 vom: 05. Feb., Seite 863-869 (DE-627)385615434 (DE-600)2143009-3 1869-2044 nnns volume:61 year:2011 number:4 day:05 month:02 pages:863-869 https://dx.doi.org/10.1007/s13213-011-0207-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_22 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_63 GBV_ILN_70 GBV_ILN_95 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_187 GBV_ILN_285 GBV_ILN_370 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4313 GBV_ILN_4328 GBV_ILN_4333 AR 61 2011 4 05 02 863-869
allfieldsSound	10.1007/s13213-011-0207-8 doi (DE-627)SPR030876613 (SPR)s13213-011-0207-8-e DE-627 ger DE-627 rakwb eng Puligundla, Pradeep verfasserin aut Repeated batch ethanolic fermentation of very high gravity medium by immobilized Saccharomyces cerevisiae 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag and the University of Milan 2011 Abstract The main objective of this study was to evaluate the effect of yeast immobilization on ethanolic fermentation of very high gravity (VHG) medium and to determine the concentrations of yeast storage carbohydrates like trehalose and glycogen during the process. Repeated batch ethanolic fermentation of VHG medium was carried out using Saccharomyces cerevisiae immobilized separately within Ca-alginate and κ-carrageenan polymers. Immobilization yields (YI) were between 80 and 90% and ethanol yields ($ Y_{P/S} $) were more than 0.41 with both carriers. An average fermentation efficiency of nearly 70% was observed in 48-h fermentation batches. Compared to free cells, a reduction of more than 50% in the accumulated trehalose, and a two-fold increase in intracellular glycogen levels were observed in immobilized yeast cells at 24 and 48 h of fermentation, respectively, with both carriers. The increased viability (up to four-fold higher) upon 18% ethanol treatment for 2 h, and the sustained viability over four successive batches of immobilized cells showed the protective nature of the polymer carriers. The chemical nature of the carriers was not found to have any adverse effect on ethanol yields. Application of immobilized yeast in porous matrices may serve as a feasible and better technique for ethanol production, at both pilot and industrial scale. Immobilization (dpeaa)DE-He213 Repeated batch fermentation (dpeaa)DE-He213 Very high gravity medium (dpeaa)DE-He213 Poludasu, Rama Mohan aut Rai, Jithan Kumar aut Obulam, Vijaya Sarathi Reddy aut Enthalten in Annals of microbiology Berlin : Springer, 1998 61(2011), 4 vom: 05. Feb., Seite 863-869 (DE-627)385615434 (DE-600)2143009-3 1869-2044 nnns volume:61 year:2011 number:4 day:05 month:02 pages:863-869 https://dx.doi.org/10.1007/s13213-011-0207-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_22 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_63 GBV_ILN_70 GBV_ILN_95 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_187 GBV_ILN_285 GBV_ILN_370 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4313 GBV_ILN_4328 GBV_ILN_4333 AR 61 2011 4 05 02 863-869
language	English
source	Enthalten in Annals of microbiology 61(2011), 4 vom: 05. Feb., Seite 863-869 volume:61 year:2011 number:4 day:05 month:02 pages:863-869
sourceStr	Enthalten in Annals of microbiology 61(2011), 4 vom: 05. Feb., Seite 863-869 volume:61 year:2011 number:4 day:05 month:02 pages:863-869
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topic_title	Repeated batch ethanolic fermentation of very high gravity medium by immobilized Saccharomyces cerevisiae Immobilization (dpeaa)DE-He213 Repeated batch fermentation (dpeaa)DE-He213 Very high gravity medium (dpeaa)DE-He213
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title	Repeated batch ethanolic fermentation of very high gravity medium by immobilized Saccharomyces cerevisiae
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title_full	Repeated batch ethanolic fermentation of very high gravity medium by immobilized Saccharomyces cerevisiae
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title_sort	repeated batch ethanolic fermentation of very high gravity medium by immobilized saccharomyces cerevisiae
title_auth	Repeated batch ethanolic fermentation of very high gravity medium by immobilized Saccharomyces cerevisiae
abstract	Abstract The main objective of this study was to evaluate the effect of yeast immobilization on ethanolic fermentation of very high gravity (VHG) medium and to determine the concentrations of yeast storage carbohydrates like trehalose and glycogen during the process. Repeated batch ethanolic fermentation of VHG medium was carried out using Saccharomyces cerevisiae immobilized separately within Ca-alginate and κ-carrageenan polymers. Immobilization yields (YI) were between 80 and 90% and ethanol yields ($ Y_{P/S} $) were more than 0.41 with both carriers. An average fermentation efficiency of nearly 70% was observed in 48-h fermentation batches. Compared to free cells, a reduction of more than 50% in the accumulated trehalose, and a two-fold increase in intracellular glycogen levels were observed in immobilized yeast cells at 24 and 48 h of fermentation, respectively, with both carriers. The increased viability (up to four-fold higher) upon 18% ethanol treatment for 2 h, and the sustained viability over four successive batches of immobilized cells showed the protective nature of the polymer carriers. The chemical nature of the carriers was not found to have any adverse effect on ethanol yields. Application of immobilized yeast in porous matrices may serve as a feasible and better technique for ethanol production, at both pilot and industrial scale. © Springer-Verlag and the University of Milan 2011
abstractGer	Abstract The main objective of this study was to evaluate the effect of yeast immobilization on ethanolic fermentation of very high gravity (VHG) medium and to determine the concentrations of yeast storage carbohydrates like trehalose and glycogen during the process. Repeated batch ethanolic fermentation of VHG medium was carried out using Saccharomyces cerevisiae immobilized separately within Ca-alginate and κ-carrageenan polymers. Immobilization yields (YI) were between 80 and 90% and ethanol yields ($ Y_{P/S} $) were more than 0.41 with both carriers. An average fermentation efficiency of nearly 70% was observed in 48-h fermentation batches. Compared to free cells, a reduction of more than 50% in the accumulated trehalose, and a two-fold increase in intracellular glycogen levels were observed in immobilized yeast cells at 24 and 48 h of fermentation, respectively, with both carriers. The increased viability (up to four-fold higher) upon 18% ethanol treatment for 2 h, and the sustained viability over four successive batches of immobilized cells showed the protective nature of the polymer carriers. The chemical nature of the carriers was not found to have any adverse effect on ethanol yields. Application of immobilized yeast in porous matrices may serve as a feasible and better technique for ethanol production, at both pilot and industrial scale. © Springer-Verlag and the University of Milan 2011
abstract_unstemmed	Abstract The main objective of this study was to evaluate the effect of yeast immobilization on ethanolic fermentation of very high gravity (VHG) medium and to determine the concentrations of yeast storage carbohydrates like trehalose and glycogen during the process. Repeated batch ethanolic fermentation of VHG medium was carried out using Saccharomyces cerevisiae immobilized separately within Ca-alginate and κ-carrageenan polymers. Immobilization yields (YI) were between 80 and 90% and ethanol yields ($ Y_{P/S} $) were more than 0.41 with both carriers. An average fermentation efficiency of nearly 70% was observed in 48-h fermentation batches. Compared to free cells, a reduction of more than 50% in the accumulated trehalose, and a two-fold increase in intracellular glycogen levels were observed in immobilized yeast cells at 24 and 48 h of fermentation, respectively, with both carriers. The increased viability (up to four-fold higher) upon 18% ethanol treatment for 2 h, and the sustained viability over four successive batches of immobilized cells showed the protective nature of the polymer carriers. The chemical nature of the carriers was not found to have any adverse effect on ethanol yields. Application of immobilized yeast in porous matrices may serve as a feasible and better technique for ethanol production, at both pilot and industrial scale. © Springer-Verlag and the University of Milan 2011
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title_short	Repeated batch ethanolic fermentation of very high gravity medium by immobilized Saccharomyces cerevisiae
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Application of immobilized yeast in porous matrices may serve as a feasible and better technique for ethanol production, at both pilot and industrial scale.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Immobilization</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Repeated batch fermentation</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Very high gravity medium</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Poludasu, Rama Mohan</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Rai, Jithan Kumar</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Obulam, Vijaya Sarathi Reddy</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Annals of microbiology</subfield><subfield code="d">Berlin : Springer, 1998</subfield><subfield code="g">61(2011), 4 vom: 05. 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Repeated batch ethanolic fermentation of very high gravity medium by immobilized Saccharomyces cerevisiae

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