Genome shuffling of Trichoderma viride for enhanced cellulase production

Abstract Genome shuffling is an efficient approach for the rapid engineering of microbial strains with desirable industrial phenotypes. In this study, we used genome shuffling in an attempt to improve the cellulase production of the wild-type strain Trichoderma viride TL-124. The initial mutant popu...
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Autor*in:	Xu, Feng [verfasserIn] Jin, Haojie Li, Huamin Tao, Li Wang, Jianping Lv, Jing Chen, Sanfeng

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2011

Schlagwörter:	Cellulase Genome shuffling RAPD

Anmerkung:	© Springer-Verlag and the University of Milan 2011

Übergeordnetes Werk:	Enthalten in: Annals of microbiology - Berlin : Springer, 1998, 62(2011), 2 vom: 25. Mai, Seite 509-515
Übergeordnetes Werk:	volume:62 ; year:2011 ; number:2 ; day:25 ; month:05 ; pages:509-515

Links:	Volltext

DOI / URN:	10.1007/s13213-011-0284-8

Katalog-ID:	SPR030877490

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allfields	10.1007/s13213-011-0284-8 doi (DE-627)SPR030877490 (SPR)s13213-011-0284-8-e DE-627 ger DE-627 rakwb eng Xu, Feng verfasserin aut Genome shuffling of Trichoderma viride for enhanced cellulase production 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag and the University of Milan 2011 Abstract Genome shuffling is an efficient approach for the rapid engineering of microbial strains with desirable industrial phenotypes. In this study, we used genome shuffling in an attempt to improve the cellulase production of the wild-type strain Trichoderma viride TL-124. The initial mutant populations were generated by ultraviolet (UV) irradiation, low-energy ion beam implantation and atmospheric pressure non-equilibrium discharge plasma (APNEDP). The mutants were subjected to recursive protoplast fusion. The cellulase activities of shuffled strains were assayed after solid-state fermentation using wheat straw, an economical raw material, as substrate. The shuffled strain T. viride F161, which was selected from among about 2,000 shuffled strains after two rounds of genome shuffling, exhibited a total cellulase activity of 4.17 U $ g^{-1} $ dry weight, which was 1.97-fold higher that of wild-type strain T. viride TL-124 (2.12 U $ g^{-1} $ dry weight). Analysis by random amplification of polymorphic DNA revealed genetic differences between the shuffled strains and the wild-type strain. Cellulase (dpeaa)DE-He213 Genome shuffling (dpeaa)DE-He213 RAPD (dpeaa)DE-He213 Jin, Haojie aut Li, Huamin aut Tao, Li aut Wang, Jianping aut Lv, Jing aut Chen, Sanfeng aut Enthalten in Annals of microbiology Berlin : Springer, 1998 62(2011), 2 vom: 25. Mai, Seite 509-515 (DE-627)385615434 (DE-600)2143009-3 1869-2044 nnns volume:62 year:2011 number:2 day:25 month:05 pages:509-515 https://dx.doi.org/10.1007/s13213-011-0284-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_22 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_63 GBV_ILN_70 GBV_ILN_95 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_187 GBV_ILN_285 GBV_ILN_370 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4313 GBV_ILN_4328 GBV_ILN_4333 AR 62 2011 2 25 05 509-515
spelling	10.1007/s13213-011-0284-8 doi (DE-627)SPR030877490 (SPR)s13213-011-0284-8-e DE-627 ger DE-627 rakwb eng Xu, Feng verfasserin aut Genome shuffling of Trichoderma viride for enhanced cellulase production 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag and the University of Milan 2011 Abstract Genome shuffling is an efficient approach for the rapid engineering of microbial strains with desirable industrial phenotypes. In this study, we used genome shuffling in an attempt to improve the cellulase production of the wild-type strain Trichoderma viride TL-124. The initial mutant populations were generated by ultraviolet (UV) irradiation, low-energy ion beam implantation and atmospheric pressure non-equilibrium discharge plasma (APNEDP). The mutants were subjected to recursive protoplast fusion. The cellulase activities of shuffled strains were assayed after solid-state fermentation using wheat straw, an economical raw material, as substrate. The shuffled strain T. viride F161, which was selected from among about 2,000 shuffled strains after two rounds of genome shuffling, exhibited a total cellulase activity of 4.17 U $ g^{-1} $ dry weight, which was 1.97-fold higher that of wild-type strain T. viride TL-124 (2.12 U $ g^{-1} $ dry weight). Analysis by random amplification of polymorphic DNA revealed genetic differences between the shuffled strains and the wild-type strain. Cellulase (dpeaa)DE-He213 Genome shuffling (dpeaa)DE-He213 RAPD (dpeaa)DE-He213 Jin, Haojie aut Li, Huamin aut Tao, Li aut Wang, Jianping aut Lv, Jing aut Chen, Sanfeng aut Enthalten in Annals of microbiology Berlin : Springer, 1998 62(2011), 2 vom: 25. Mai, Seite 509-515 (DE-627)385615434 (DE-600)2143009-3 1869-2044 nnns volume:62 year:2011 number:2 day:25 month:05 pages:509-515 https://dx.doi.org/10.1007/s13213-011-0284-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_22 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_63 GBV_ILN_70 GBV_ILN_95 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_187 GBV_ILN_285 GBV_ILN_370 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4313 GBV_ILN_4328 GBV_ILN_4333 AR 62 2011 2 25 05 509-515
allfields_unstemmed	10.1007/s13213-011-0284-8 doi (DE-627)SPR030877490 (SPR)s13213-011-0284-8-e DE-627 ger DE-627 rakwb eng Xu, Feng verfasserin aut Genome shuffling of Trichoderma viride for enhanced cellulase production 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag and the University of Milan 2011 Abstract Genome shuffling is an efficient approach for the rapid engineering of microbial strains with desirable industrial phenotypes. In this study, we used genome shuffling in an attempt to improve the cellulase production of the wild-type strain Trichoderma viride TL-124. The initial mutant populations were generated by ultraviolet (UV) irradiation, low-energy ion beam implantation and atmospheric pressure non-equilibrium discharge plasma (APNEDP). The mutants were subjected to recursive protoplast fusion. The cellulase activities of shuffled strains were assayed after solid-state fermentation using wheat straw, an economical raw material, as substrate. The shuffled strain T. viride F161, which was selected from among about 2,000 shuffled strains after two rounds of genome shuffling, exhibited a total cellulase activity of 4.17 U $ g^{-1} $ dry weight, which was 1.97-fold higher that of wild-type strain T. viride TL-124 (2.12 U $ g^{-1} $ dry weight). Analysis by random amplification of polymorphic DNA revealed genetic differences between the shuffled strains and the wild-type strain. Cellulase (dpeaa)DE-He213 Genome shuffling (dpeaa)DE-He213 RAPD (dpeaa)DE-He213 Jin, Haojie aut Li, Huamin aut Tao, Li aut Wang, Jianping aut Lv, Jing aut Chen, Sanfeng aut Enthalten in Annals of microbiology Berlin : Springer, 1998 62(2011), 2 vom: 25. Mai, Seite 509-515 (DE-627)385615434 (DE-600)2143009-3 1869-2044 nnns volume:62 year:2011 number:2 day:25 month:05 pages:509-515 https://dx.doi.org/10.1007/s13213-011-0284-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_22 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_63 GBV_ILN_70 GBV_ILN_95 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_187 GBV_ILN_285 GBV_ILN_370 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4313 GBV_ILN_4328 GBV_ILN_4333 AR 62 2011 2 25 05 509-515
allfieldsGer	10.1007/s13213-011-0284-8 doi (DE-627)SPR030877490 (SPR)s13213-011-0284-8-e DE-627 ger DE-627 rakwb eng Xu, Feng verfasserin aut Genome shuffling of Trichoderma viride for enhanced cellulase production 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag and the University of Milan 2011 Abstract Genome shuffling is an efficient approach for the rapid engineering of microbial strains with desirable industrial phenotypes. In this study, we used genome shuffling in an attempt to improve the cellulase production of the wild-type strain Trichoderma viride TL-124. The initial mutant populations were generated by ultraviolet (UV) irradiation, low-energy ion beam implantation and atmospheric pressure non-equilibrium discharge plasma (APNEDP). The mutants were subjected to recursive protoplast fusion. The cellulase activities of shuffled strains were assayed after solid-state fermentation using wheat straw, an economical raw material, as substrate. The shuffled strain T. viride F161, which was selected from among about 2,000 shuffled strains after two rounds of genome shuffling, exhibited a total cellulase activity of 4.17 U $ g^{-1} $ dry weight, which was 1.97-fold higher that of wild-type strain T. viride TL-124 (2.12 U $ g^{-1} $ dry weight). Analysis by random amplification of polymorphic DNA revealed genetic differences between the shuffled strains and the wild-type strain. Cellulase (dpeaa)DE-He213 Genome shuffling (dpeaa)DE-He213 RAPD (dpeaa)DE-He213 Jin, Haojie aut Li, Huamin aut Tao, Li aut Wang, Jianping aut Lv, Jing aut Chen, Sanfeng aut Enthalten in Annals of microbiology Berlin : Springer, 1998 62(2011), 2 vom: 25. Mai, Seite 509-515 (DE-627)385615434 (DE-600)2143009-3 1869-2044 nnns volume:62 year:2011 number:2 day:25 month:05 pages:509-515 https://dx.doi.org/10.1007/s13213-011-0284-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_22 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_63 GBV_ILN_70 GBV_ILN_95 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_187 GBV_ILN_285 GBV_ILN_370 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4313 GBV_ILN_4328 GBV_ILN_4333 AR 62 2011 2 25 05 509-515
allfieldsSound	10.1007/s13213-011-0284-8 doi (DE-627)SPR030877490 (SPR)s13213-011-0284-8-e DE-627 ger DE-627 rakwb eng Xu, Feng verfasserin aut Genome shuffling of Trichoderma viride for enhanced cellulase production 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag and the University of Milan 2011 Abstract Genome shuffling is an efficient approach for the rapid engineering of microbial strains with desirable industrial phenotypes. In this study, we used genome shuffling in an attempt to improve the cellulase production of the wild-type strain Trichoderma viride TL-124. The initial mutant populations were generated by ultraviolet (UV) irradiation, low-energy ion beam implantation and atmospheric pressure non-equilibrium discharge plasma (APNEDP). The mutants were subjected to recursive protoplast fusion. The cellulase activities of shuffled strains were assayed after solid-state fermentation using wheat straw, an economical raw material, as substrate. The shuffled strain T. viride F161, which was selected from among about 2,000 shuffled strains after two rounds of genome shuffling, exhibited a total cellulase activity of 4.17 U $ g^{-1} $ dry weight, which was 1.97-fold higher that of wild-type strain T. viride TL-124 (2.12 U $ g^{-1} $ dry weight). Analysis by random amplification of polymorphic DNA revealed genetic differences between the shuffled strains and the wild-type strain. Cellulase (dpeaa)DE-He213 Genome shuffling (dpeaa)DE-He213 RAPD (dpeaa)DE-He213 Jin, Haojie aut Li, Huamin aut Tao, Li aut Wang, Jianping aut Lv, Jing aut Chen, Sanfeng aut Enthalten in Annals of microbiology Berlin : Springer, 1998 62(2011), 2 vom: 25. Mai, Seite 509-515 (DE-627)385615434 (DE-600)2143009-3 1869-2044 nnns volume:62 year:2011 number:2 day:25 month:05 pages:509-515 https://dx.doi.org/10.1007/s13213-011-0284-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_22 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_63 GBV_ILN_70 GBV_ILN_95 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_187 GBV_ILN_285 GBV_ILN_370 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4313 GBV_ILN_4328 GBV_ILN_4333 AR 62 2011 2 25 05 509-515
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source	Enthalten in Annals of microbiology 62(2011), 2 vom: 25. Mai, Seite 509-515 volume:62 year:2011 number:2 day:25 month:05 pages:509-515
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authorswithroles_txt_mv	Xu, Feng @@aut@@ Jin, Haojie @@aut@@ Li, Huamin @@aut@@ Tao, Li @@aut@@ Wang, Jianping @@aut@@ Lv, Jing @@aut@@ Chen, Sanfeng @@aut@@
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topic_title	Genome shuffling of Trichoderma viride for enhanced cellulase production Cellulase (dpeaa)DE-He213 Genome shuffling (dpeaa)DE-He213 RAPD (dpeaa)DE-He213
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title	Genome shuffling of Trichoderma viride for enhanced cellulase production
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title_full	Genome shuffling of Trichoderma viride for enhanced cellulase production
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author_browse	Xu, Feng Jin, Haojie Li, Huamin Tao, Li Wang, Jianping Lv, Jing Chen, Sanfeng
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title_sort	genome shuffling of trichoderma viride for enhanced cellulase production
title_auth	Genome shuffling of Trichoderma viride for enhanced cellulase production
abstract	Abstract Genome shuffling is an efficient approach for the rapid engineering of microbial strains with desirable industrial phenotypes. In this study, we used genome shuffling in an attempt to improve the cellulase production of the wild-type strain Trichoderma viride TL-124. The initial mutant populations were generated by ultraviolet (UV) irradiation, low-energy ion beam implantation and atmospheric pressure non-equilibrium discharge plasma (APNEDP). The mutants were subjected to recursive protoplast fusion. The cellulase activities of shuffled strains were assayed after solid-state fermentation using wheat straw, an economical raw material, as substrate. The shuffled strain T. viride F161, which was selected from among about 2,000 shuffled strains after two rounds of genome shuffling, exhibited a total cellulase activity of 4.17 U $ g^{-1} $ dry weight, which was 1.97-fold higher that of wild-type strain T. viride TL-124 (2.12 U $ g^{-1} $ dry weight). Analysis by random amplification of polymorphic DNA revealed genetic differences between the shuffled strains and the wild-type strain. © Springer-Verlag and the University of Milan 2011
abstractGer	Abstract Genome shuffling is an efficient approach for the rapid engineering of microbial strains with desirable industrial phenotypes. In this study, we used genome shuffling in an attempt to improve the cellulase production of the wild-type strain Trichoderma viride TL-124. The initial mutant populations were generated by ultraviolet (UV) irradiation, low-energy ion beam implantation and atmospheric pressure non-equilibrium discharge plasma (APNEDP). The mutants were subjected to recursive protoplast fusion. The cellulase activities of shuffled strains were assayed after solid-state fermentation using wheat straw, an economical raw material, as substrate. The shuffled strain T. viride F161, which was selected from among about 2,000 shuffled strains after two rounds of genome shuffling, exhibited a total cellulase activity of 4.17 U $ g^{-1} $ dry weight, which was 1.97-fold higher that of wild-type strain T. viride TL-124 (2.12 U $ g^{-1} $ dry weight). Analysis by random amplification of polymorphic DNA revealed genetic differences between the shuffled strains and the wild-type strain. © Springer-Verlag and the University of Milan 2011
abstract_unstemmed	Abstract Genome shuffling is an efficient approach for the rapid engineering of microbial strains with desirable industrial phenotypes. In this study, we used genome shuffling in an attempt to improve the cellulase production of the wild-type strain Trichoderma viride TL-124. The initial mutant populations were generated by ultraviolet (UV) irradiation, low-energy ion beam implantation and atmospheric pressure non-equilibrium discharge plasma (APNEDP). The mutants were subjected to recursive protoplast fusion. The cellulase activities of shuffled strains were assayed after solid-state fermentation using wheat straw, an economical raw material, as substrate. The shuffled strain T. viride F161, which was selected from among about 2,000 shuffled strains after two rounds of genome shuffling, exhibited a total cellulase activity of 4.17 U $ g^{-1} $ dry weight, which was 1.97-fold higher that of wild-type strain T. viride TL-124 (2.12 U $ g^{-1} $ dry weight). Analysis by random amplification of polymorphic DNA revealed genetic differences between the shuffled strains and the wild-type strain. © Springer-Verlag and the University of Milan 2011
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title_short	Genome shuffling of Trichoderma viride for enhanced cellulase production
url	https://dx.doi.org/10.1007/s13213-011-0284-8
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author2	Jin, Haojie Li, Huamin Tao, Li Wang, Jianping Lv, Jing Chen, Sanfeng
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up_date	2024-07-03T20:39:44.295Z
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In this study, we used genome shuffling in an attempt to improve the cellulase production of the wild-type strain Trichoderma viride TL-124. The initial mutant populations were generated by ultraviolet (UV) irradiation, low-energy ion beam implantation and atmospheric pressure non-equilibrium discharge plasma (APNEDP). The mutants were subjected to recursive protoplast fusion. The cellulase activities of shuffled strains were assayed after solid-state fermentation using wheat straw, an economical raw material, as substrate. The shuffled strain T. viride F161, which was selected from among about 2,000 shuffled strains after two rounds of genome shuffling, exhibited a total cellulase activity of 4.17 U $ g^{-1} $ dry weight, which was 1.97-fold higher that of wild-type strain T. viride TL-124 (2.12 U $ g^{-1} $ dry weight). 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Genome shuffling of Trichoderma viride for enhanced cellulase production

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