Prevalence of toxicogenic bacteria in some foods and detection of Bacillus cereus and Staphylococcus aureus enterotoxin genes using multiplex PCR

Abstract Thirty-three food samples representing seven different food products were collected from the market in Sharkia Governorate (Egypt) and analyzed for their bacterial burden, including total mesophilic bacteria, spore formers, Staphylococcus aureus, and Bacillus cereus, using specific and sele...
Ausführliche Beschreibung

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Autor*in:	Abdou, Mohamed A. [verfasserIn] Awny, Nadia Mohammed Abozeid, Azza Abl-Elaziz M.

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2011

Schlagwörter:	Food-borne pathogens Virulence factors Protein profile Enterotoxin genes

Anmerkung:	© Springer-Verlag and the University of Milan 2011

Übergeordnetes Werk:	Enthalten in: Annals of microbiology - Berlin : Springer, 1998, 62(2011), 2 vom: 22. Juni, Seite 569-580
Übergeordnetes Werk:	volume:62 ; year:2011 ; number:2 ; day:22 ; month:06 ; pages:569-580

Links:	Volltext

DOI / URN:	10.1007/s13213-011-0293-7

Katalog-ID:	SPR030877598

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author_variant	m a a ma maa n m a nm nma a a e m a aaem aaema
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allfields	10.1007/s13213-011-0293-7 doi (DE-627)SPR030877598 (SPR)s13213-011-0293-7-e DE-627 ger DE-627 rakwb eng Abdou, Mohamed A. verfasserin aut Prevalence of toxicogenic bacteria in some foods and detection of Bacillus cereus and Staphylococcus aureus enterotoxin genes using multiplex PCR 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag and the University of Milan 2011 Abstract Thirty-three food samples representing seven different food products were collected from the market in Sharkia Governorate (Egypt) and analyzed for their bacterial burden, including total mesophilic bacteria, spore formers, Staphylococcus aureus, and Bacillus cereus, using specific and selective nutrient media. The identified strains were screened for their virulence factors using the agar diffusion method. B. cereus strains CH, GT1, LB3, and G8 were found to be the most potent isolates, with four S. aureus showing nearly equal potency in terms of the virulence factors investigated. Separation of the extracellular proteins of the four most potent B. cereus strains by sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed the presence of protein bands with molecular weights ranging from 30 to 53 kDa that were suspected to be hemolytic enterotoxins. Protein bands having molecular weights between 22 and 33 kDa were also observed in three strains (S1, S2, and S3) of the S. aureus strains examined. Applying the multiplex PCR technique, we used two pairs of primers (FHblC and RHblC; FCytK and R2Cytk) to detect the toxin genes (hblC and cytK) in the suspected toxic B. cereus strains and five pairs of primers (SEA-3 and SEA-4; SEB-1 and SEB-2; SEC-5 and SEC-6; SED-1 and SED-2; SEE-1and SEE-2) to detect the five enterotoxins in the S. aureus strains. Our results indicate that the multiplex PCR amplification enabled the rapid detection and identification of enterotoxin genes in food-borne bacteria. Food-borne pathogens (dpeaa)DE-He213 Virulence factors (dpeaa)DE-He213 Protein profile (dpeaa)DE-He213 Enterotoxin genes (dpeaa)DE-He213 Awny, Nadia Mohammed aut Abozeid, Azza Abl-Elaziz M. aut Enthalten in Annals of microbiology Berlin : Springer, 1998 62(2011), 2 vom: 22. Juni, Seite 569-580 (DE-627)385615434 (DE-600)2143009-3 1869-2044 nnns volume:62 year:2011 number:2 day:22 month:06 pages:569-580 https://dx.doi.org/10.1007/s13213-011-0293-7 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_22 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_63 GBV_ILN_70 GBV_ILN_95 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_187 GBV_ILN_285 GBV_ILN_370 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4313 GBV_ILN_4328 GBV_ILN_4333 AR 62 2011 2 22 06 569-580
spelling	10.1007/s13213-011-0293-7 doi (DE-627)SPR030877598 (SPR)s13213-011-0293-7-e DE-627 ger DE-627 rakwb eng Abdou, Mohamed A. verfasserin aut Prevalence of toxicogenic bacteria in some foods and detection of Bacillus cereus and Staphylococcus aureus enterotoxin genes using multiplex PCR 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag and the University of Milan 2011 Abstract Thirty-three food samples representing seven different food products were collected from the market in Sharkia Governorate (Egypt) and analyzed for their bacterial burden, including total mesophilic bacteria, spore formers, Staphylococcus aureus, and Bacillus cereus, using specific and selective nutrient media. The identified strains were screened for their virulence factors using the agar diffusion method. B. cereus strains CH, GT1, LB3, and G8 were found to be the most potent isolates, with four S. aureus showing nearly equal potency in terms of the virulence factors investigated. Separation of the extracellular proteins of the four most potent B. cereus strains by sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed the presence of protein bands with molecular weights ranging from 30 to 53 kDa that were suspected to be hemolytic enterotoxins. Protein bands having molecular weights between 22 and 33 kDa were also observed in three strains (S1, S2, and S3) of the S. aureus strains examined. Applying the multiplex PCR technique, we used two pairs of primers (FHblC and RHblC; FCytK and R2Cytk) to detect the toxin genes (hblC and cytK) in the suspected toxic B. cereus strains and five pairs of primers (SEA-3 and SEA-4; SEB-1 and SEB-2; SEC-5 and SEC-6; SED-1 and SED-2; SEE-1and SEE-2) to detect the five enterotoxins in the S. aureus strains. Our results indicate that the multiplex PCR amplification enabled the rapid detection and identification of enterotoxin genes in food-borne bacteria. Food-borne pathogens (dpeaa)DE-He213 Virulence factors (dpeaa)DE-He213 Protein profile (dpeaa)DE-He213 Enterotoxin genes (dpeaa)DE-He213 Awny, Nadia Mohammed aut Abozeid, Azza Abl-Elaziz M. aut Enthalten in Annals of microbiology Berlin : Springer, 1998 62(2011), 2 vom: 22. Juni, Seite 569-580 (DE-627)385615434 (DE-600)2143009-3 1869-2044 nnns volume:62 year:2011 number:2 day:22 month:06 pages:569-580 https://dx.doi.org/10.1007/s13213-011-0293-7 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_22 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_63 GBV_ILN_70 GBV_ILN_95 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_187 GBV_ILN_285 GBV_ILN_370 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4313 GBV_ILN_4328 GBV_ILN_4333 AR 62 2011 2 22 06 569-580
allfields_unstemmed	10.1007/s13213-011-0293-7 doi (DE-627)SPR030877598 (SPR)s13213-011-0293-7-e DE-627 ger DE-627 rakwb eng Abdou, Mohamed A. verfasserin aut Prevalence of toxicogenic bacteria in some foods and detection of Bacillus cereus and Staphylococcus aureus enterotoxin genes using multiplex PCR 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag and the University of Milan 2011 Abstract Thirty-three food samples representing seven different food products were collected from the market in Sharkia Governorate (Egypt) and analyzed for their bacterial burden, including total mesophilic bacteria, spore formers, Staphylococcus aureus, and Bacillus cereus, using specific and selective nutrient media. The identified strains were screened for their virulence factors using the agar diffusion method. B. cereus strains CH, GT1, LB3, and G8 were found to be the most potent isolates, with four S. aureus showing nearly equal potency in terms of the virulence factors investigated. Separation of the extracellular proteins of the four most potent B. cereus strains by sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed the presence of protein bands with molecular weights ranging from 30 to 53 kDa that were suspected to be hemolytic enterotoxins. Protein bands having molecular weights between 22 and 33 kDa were also observed in three strains (S1, S2, and S3) of the S. aureus strains examined. Applying the multiplex PCR technique, we used two pairs of primers (FHblC and RHblC; FCytK and R2Cytk) to detect the toxin genes (hblC and cytK) in the suspected toxic B. cereus strains and five pairs of primers (SEA-3 and SEA-4; SEB-1 and SEB-2; SEC-5 and SEC-6; SED-1 and SED-2; SEE-1and SEE-2) to detect the five enterotoxins in the S. aureus strains. Our results indicate that the multiplex PCR amplification enabled the rapid detection and identification of enterotoxin genes in food-borne bacteria. Food-borne pathogens (dpeaa)DE-He213 Virulence factors (dpeaa)DE-He213 Protein profile (dpeaa)DE-He213 Enterotoxin genes (dpeaa)DE-He213 Awny, Nadia Mohammed aut Abozeid, Azza Abl-Elaziz M. aut Enthalten in Annals of microbiology Berlin : Springer, 1998 62(2011), 2 vom: 22. Juni, Seite 569-580 (DE-627)385615434 (DE-600)2143009-3 1869-2044 nnns volume:62 year:2011 number:2 day:22 month:06 pages:569-580 https://dx.doi.org/10.1007/s13213-011-0293-7 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_22 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_63 GBV_ILN_70 GBV_ILN_95 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_187 GBV_ILN_285 GBV_ILN_370 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4313 GBV_ILN_4328 GBV_ILN_4333 AR 62 2011 2 22 06 569-580
allfieldsGer	10.1007/s13213-011-0293-7 doi (DE-627)SPR030877598 (SPR)s13213-011-0293-7-e DE-627 ger DE-627 rakwb eng Abdou, Mohamed A. verfasserin aut Prevalence of toxicogenic bacteria in some foods and detection of Bacillus cereus and Staphylococcus aureus enterotoxin genes using multiplex PCR 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag and the University of Milan 2011 Abstract Thirty-three food samples representing seven different food products were collected from the market in Sharkia Governorate (Egypt) and analyzed for their bacterial burden, including total mesophilic bacteria, spore formers, Staphylococcus aureus, and Bacillus cereus, using specific and selective nutrient media. The identified strains were screened for their virulence factors using the agar diffusion method. B. cereus strains CH, GT1, LB3, and G8 were found to be the most potent isolates, with four S. aureus showing nearly equal potency in terms of the virulence factors investigated. Separation of the extracellular proteins of the four most potent B. cereus strains by sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed the presence of protein bands with molecular weights ranging from 30 to 53 kDa that were suspected to be hemolytic enterotoxins. Protein bands having molecular weights between 22 and 33 kDa were also observed in three strains (S1, S2, and S3) of the S. aureus strains examined. Applying the multiplex PCR technique, we used two pairs of primers (FHblC and RHblC; FCytK and R2Cytk) to detect the toxin genes (hblC and cytK) in the suspected toxic B. cereus strains and five pairs of primers (SEA-3 and SEA-4; SEB-1 and SEB-2; SEC-5 and SEC-6; SED-1 and SED-2; SEE-1and SEE-2) to detect the five enterotoxins in the S. aureus strains. Our results indicate that the multiplex PCR amplification enabled the rapid detection and identification of enterotoxin genes in food-borne bacteria. Food-borne pathogens (dpeaa)DE-He213 Virulence factors (dpeaa)DE-He213 Protein profile (dpeaa)DE-He213 Enterotoxin genes (dpeaa)DE-He213 Awny, Nadia Mohammed aut Abozeid, Azza Abl-Elaziz M. aut Enthalten in Annals of microbiology Berlin : Springer, 1998 62(2011), 2 vom: 22. Juni, Seite 569-580 (DE-627)385615434 (DE-600)2143009-3 1869-2044 nnns volume:62 year:2011 number:2 day:22 month:06 pages:569-580 https://dx.doi.org/10.1007/s13213-011-0293-7 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_22 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_63 GBV_ILN_70 GBV_ILN_95 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_187 GBV_ILN_285 GBV_ILN_370 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4313 GBV_ILN_4328 GBV_ILN_4333 AR 62 2011 2 22 06 569-580
allfieldsSound	10.1007/s13213-011-0293-7 doi (DE-627)SPR030877598 (SPR)s13213-011-0293-7-e DE-627 ger DE-627 rakwb eng Abdou, Mohamed A. verfasserin aut Prevalence of toxicogenic bacteria in some foods and detection of Bacillus cereus and Staphylococcus aureus enterotoxin genes using multiplex PCR 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag and the University of Milan 2011 Abstract Thirty-three food samples representing seven different food products were collected from the market in Sharkia Governorate (Egypt) and analyzed for their bacterial burden, including total mesophilic bacteria, spore formers, Staphylococcus aureus, and Bacillus cereus, using specific and selective nutrient media. The identified strains were screened for their virulence factors using the agar diffusion method. B. cereus strains CH, GT1, LB3, and G8 were found to be the most potent isolates, with four S. aureus showing nearly equal potency in terms of the virulence factors investigated. Separation of the extracellular proteins of the four most potent B. cereus strains by sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed the presence of protein bands with molecular weights ranging from 30 to 53 kDa that were suspected to be hemolytic enterotoxins. Protein bands having molecular weights between 22 and 33 kDa were also observed in three strains (S1, S2, and S3) of the S. aureus strains examined. Applying the multiplex PCR technique, we used two pairs of primers (FHblC and RHblC; FCytK and R2Cytk) to detect the toxin genes (hblC and cytK) in the suspected toxic B. cereus strains and five pairs of primers (SEA-3 and SEA-4; SEB-1 and SEB-2; SEC-5 and SEC-6; SED-1 and SED-2; SEE-1and SEE-2) to detect the five enterotoxins in the S. aureus strains. Our results indicate that the multiplex PCR amplification enabled the rapid detection and identification of enterotoxin genes in food-borne bacteria. Food-borne pathogens (dpeaa)DE-He213 Virulence factors (dpeaa)DE-He213 Protein profile (dpeaa)DE-He213 Enterotoxin genes (dpeaa)DE-He213 Awny, Nadia Mohammed aut Abozeid, Azza Abl-Elaziz M. aut Enthalten in Annals of microbiology Berlin : Springer, 1998 62(2011), 2 vom: 22. Juni, Seite 569-580 (DE-627)385615434 (DE-600)2143009-3 1869-2044 nnns volume:62 year:2011 number:2 day:22 month:06 pages:569-580 https://dx.doi.org/10.1007/s13213-011-0293-7 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_22 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_63 GBV_ILN_70 GBV_ILN_95 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_187 GBV_ILN_285 GBV_ILN_370 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4313 GBV_ILN_4328 GBV_ILN_4333 AR 62 2011 2 22 06 569-580
language	English
source	Enthalten in Annals of microbiology 62(2011), 2 vom: 22. Juni, Seite 569-580 volume:62 year:2011 number:2 day:22 month:06 pages:569-580
sourceStr	Enthalten in Annals of microbiology 62(2011), 2 vom: 22. Juni, Seite 569-580 volume:62 year:2011 number:2 day:22 month:06 pages:569-580
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Food-borne pathogens Virulence factors Protein profile Enterotoxin genes
isfreeaccess_bool	false
container_title	Annals of microbiology
authorswithroles_txt_mv	Abdou, Mohamed A. @@aut@@ Awny, Nadia Mohammed @@aut@@ Abozeid, Azza Abl-Elaziz M. @@aut@@
publishDateDaySort_date	2011-06-22T00:00:00Z
hierarchy_top_id	385615434
id	SPR030877598
language_de	englisch
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author	Abdou, Mohamed A.
spellingShingle	Abdou, Mohamed A. misc Food-borne pathogens misc Virulence factors misc Protein profile misc Enterotoxin genes Prevalence of toxicogenic bacteria in some foods and detection of Bacillus cereus and Staphylococcus aureus enterotoxin genes using multiplex PCR
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topic_title	Prevalence of toxicogenic bacteria in some foods and detection of Bacillus cereus and Staphylococcus aureus enterotoxin genes using multiplex PCR Food-borne pathogens (dpeaa)DE-He213 Virulence factors (dpeaa)DE-He213 Protein profile (dpeaa)DE-He213 Enterotoxin genes (dpeaa)DE-He213
topic	misc Food-borne pathogens misc Virulence factors misc Protein profile misc Enterotoxin genes
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title	Prevalence of toxicogenic bacteria in some foods and detection of Bacillus cereus and Staphylococcus aureus enterotoxin genes using multiplex PCR
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title_full	Prevalence of toxicogenic bacteria in some foods and detection of Bacillus cereus and Staphylococcus aureus enterotoxin genes using multiplex PCR
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author_browse	Abdou, Mohamed A. Awny, Nadia Mohammed Abozeid, Azza Abl-Elaziz M.
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title_sort	prevalence of toxicogenic bacteria in some foods and detection of bacillus cereus and staphylococcus aureus enterotoxin genes using multiplex pcr
title_auth	Prevalence of toxicogenic bacteria in some foods and detection of Bacillus cereus and Staphylococcus aureus enterotoxin genes using multiplex PCR
abstract	Abstract Thirty-three food samples representing seven different food products were collected from the market in Sharkia Governorate (Egypt) and analyzed for their bacterial burden, including total mesophilic bacteria, spore formers, Staphylococcus aureus, and Bacillus cereus, using specific and selective nutrient media. The identified strains were screened for their virulence factors using the agar diffusion method. B. cereus strains CH, GT1, LB3, and G8 were found to be the most potent isolates, with four S. aureus showing nearly equal potency in terms of the virulence factors investigated. Separation of the extracellular proteins of the four most potent B. cereus strains by sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed the presence of protein bands with molecular weights ranging from 30 to 53 kDa that were suspected to be hemolytic enterotoxins. Protein bands having molecular weights between 22 and 33 kDa were also observed in three strains (S1, S2, and S3) of the S. aureus strains examined. Applying the multiplex PCR technique, we used two pairs of primers (FHblC and RHblC; FCytK and R2Cytk) to detect the toxin genes (hblC and cytK) in the suspected toxic B. cereus strains and five pairs of primers (SEA-3 and SEA-4; SEB-1 and SEB-2; SEC-5 and SEC-6; SED-1 and SED-2; SEE-1and SEE-2) to detect the five enterotoxins in the S. aureus strains. Our results indicate that the multiplex PCR amplification enabled the rapid detection and identification of enterotoxin genes in food-borne bacteria. © Springer-Verlag and the University of Milan 2011
abstractGer	Abstract Thirty-three food samples representing seven different food products were collected from the market in Sharkia Governorate (Egypt) and analyzed for their bacterial burden, including total mesophilic bacteria, spore formers, Staphylococcus aureus, and Bacillus cereus, using specific and selective nutrient media. The identified strains were screened for their virulence factors using the agar diffusion method. B. cereus strains CH, GT1, LB3, and G8 were found to be the most potent isolates, with four S. aureus showing nearly equal potency in terms of the virulence factors investigated. Separation of the extracellular proteins of the four most potent B. cereus strains by sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed the presence of protein bands with molecular weights ranging from 30 to 53 kDa that were suspected to be hemolytic enterotoxins. Protein bands having molecular weights between 22 and 33 kDa were also observed in three strains (S1, S2, and S3) of the S. aureus strains examined. Applying the multiplex PCR technique, we used two pairs of primers (FHblC and RHblC; FCytK and R2Cytk) to detect the toxin genes (hblC and cytK) in the suspected toxic B. cereus strains and five pairs of primers (SEA-3 and SEA-4; SEB-1 and SEB-2; SEC-5 and SEC-6; SED-1 and SED-2; SEE-1and SEE-2) to detect the five enterotoxins in the S. aureus strains. Our results indicate that the multiplex PCR amplification enabled the rapid detection and identification of enterotoxin genes in food-borne bacteria. © Springer-Verlag and the University of Milan 2011
abstract_unstemmed	Abstract Thirty-three food samples representing seven different food products were collected from the market in Sharkia Governorate (Egypt) and analyzed for their bacterial burden, including total mesophilic bacteria, spore formers, Staphylococcus aureus, and Bacillus cereus, using specific and selective nutrient media. The identified strains were screened for their virulence factors using the agar diffusion method. B. cereus strains CH, GT1, LB3, and G8 were found to be the most potent isolates, with four S. aureus showing nearly equal potency in terms of the virulence factors investigated. Separation of the extracellular proteins of the four most potent B. cereus strains by sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed the presence of protein bands with molecular weights ranging from 30 to 53 kDa that were suspected to be hemolytic enterotoxins. Protein bands having molecular weights between 22 and 33 kDa were also observed in three strains (S1, S2, and S3) of the S. aureus strains examined. Applying the multiplex PCR technique, we used two pairs of primers (FHblC and RHblC; FCytK and R2Cytk) to detect the toxin genes (hblC and cytK) in the suspected toxic B. cereus strains and five pairs of primers (SEA-3 and SEA-4; SEB-1 and SEB-2; SEC-5 and SEC-6; SED-1 and SED-2; SEE-1and SEE-2) to detect the five enterotoxins in the S. aureus strains. Our results indicate that the multiplex PCR amplification enabled the rapid detection and identification of enterotoxin genes in food-borne bacteria. © Springer-Verlag and the University of Milan 2011
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title_short	Prevalence of toxicogenic bacteria in some foods and detection of Bacillus cereus and Staphylococcus aureus enterotoxin genes using multiplex PCR
url	https://dx.doi.org/10.1007/s13213-011-0293-7
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Prevalence of toxicogenic bacteria in some foods and detection of Bacillus cereus and Staphylococcus aureus enterotoxin genes using multiplex PCR

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