Culturable bacterial pool from aged petroleum-contaminated soil: identification of oil-eating Bacillus strains

Abstract Information gleaned from soil microbiota may provide access to new economically important species. Here, we describe the isolation, identification, and genetic diversity of high-density bacterial populations isolated from aged oil-contaminated soil. Twenty different morphotypes were identif...
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Gespeichert in:

Autor*in:	Granzotto, Giovana [verfasserIn] Marcelino, Paulo Ricardo Franco Barbosa, Aneli de Melo Rodrigues, Elisete Pains Rezende, Maria Inês Oliveira, André Luiz Martinez de

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2012

Schlagwörter:	Bioremediation Bioemulsifier Lipase Soil microbiology

Anmerkung:	© Springer-Verlag and the University of Milan 2012

Übergeordnetes Werk:	Enthalten in: Annals of microbiology - Berlin : Springer, 1998, 62(2012), 4 vom: 22. Feb., Seite 1681-1690
Übergeordnetes Werk:	volume:62 ; year:2012 ; number:4 ; day:22 ; month:02 ; pages:1681-1690

Links:	Volltext

DOI / URN:	10.1007/s13213-012-0425-8

Katalog-ID:	SPR030879035

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allfields	10.1007/s13213-012-0425-8 doi (DE-627)SPR030879035 (SPR)s13213-012-0425-8-e DE-627 ger DE-627 rakwb eng Granzotto, Giovana verfasserin aut Culturable bacterial pool from aged petroleum-contaminated soil: identification of oil-eating Bacillus strains 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag and the University of Milan 2012 Abstract Information gleaned from soil microbiota may provide access to new economically important species. Here, we describe the isolation, identification, and genetic diversity of high-density bacterial populations isolated from aged oil-contaminated soil. Twenty different morphotypes were identified in populations present at densities of up to $ 10^{7} $ cells $ g^{−1} $ soil, encompassing seven bacterial genera based on 16S rRNA sequencing. Six isolates of the genus Bacillus were identified, three of which appear to consume oil. The genetic clusters defined by the DNA fingerprinting analysis suggest that there is a close relationship between these oil-eating Bacillus species. Isolates able to grow using crude oil as a carbon source were biochemically characterized and found to exhibit high lipolytic activity in liquid medium and to produce alkaline-stable biosurfactants. Fluorescence spectroscopy analysis of the cell-free extract from the oil-eating Bacillus sp. strain MO.04b showed an increase in the relative fluorescence intensity of low-molecular-mass aromatics concomitantly with an increase in the protein content, suggesting the transformation of aromatic hydrocarbons to the liquid phase in response to biodegradation. The approach adopted in this study suggests a low diversity of the high-density bacterial population colonizing an aged oil-contaminated soil and may prove useful in selecting bacterial isolates for bioremediation studies and biotechnological applications such as biosurfactant production. Bioremediation (dpeaa)DE-He213 Bioemulsifier (dpeaa)DE-He213 Lipase (dpeaa)DE-He213 Soil microbiology (dpeaa)DE-He213 Marcelino, Paulo Ricardo Franco aut Barbosa, Aneli de Melo aut Rodrigues, Elisete Pains aut Rezende, Maria Inês aut Oliveira, André Luiz Martinez de aut Enthalten in Annals of microbiology Berlin : Springer, 1998 62(2012), 4 vom: 22. Feb., Seite 1681-1690 (DE-627)385615434 (DE-600)2143009-3 1869-2044 nnns volume:62 year:2012 number:4 day:22 month:02 pages:1681-1690 https://dx.doi.org/10.1007/s13213-012-0425-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_22 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_63 GBV_ILN_70 GBV_ILN_95 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_187 GBV_ILN_285 GBV_ILN_370 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4313 GBV_ILN_4328 GBV_ILN_4333 AR 62 2012 4 22 02 1681-1690
spelling	10.1007/s13213-012-0425-8 doi (DE-627)SPR030879035 (SPR)s13213-012-0425-8-e DE-627 ger DE-627 rakwb eng Granzotto, Giovana verfasserin aut Culturable bacterial pool from aged petroleum-contaminated soil: identification of oil-eating Bacillus strains 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag and the University of Milan 2012 Abstract Information gleaned from soil microbiota may provide access to new economically important species. Here, we describe the isolation, identification, and genetic diversity of high-density bacterial populations isolated from aged oil-contaminated soil. Twenty different morphotypes were identified in populations present at densities of up to $ 10^{7} $ cells $ g^{−1} $ soil, encompassing seven bacterial genera based on 16S rRNA sequencing. Six isolates of the genus Bacillus were identified, three of which appear to consume oil. The genetic clusters defined by the DNA fingerprinting analysis suggest that there is a close relationship between these oil-eating Bacillus species. Isolates able to grow using crude oil as a carbon source were biochemically characterized and found to exhibit high lipolytic activity in liquid medium and to produce alkaline-stable biosurfactants. Fluorescence spectroscopy analysis of the cell-free extract from the oil-eating Bacillus sp. strain MO.04b showed an increase in the relative fluorescence intensity of low-molecular-mass aromatics concomitantly with an increase in the protein content, suggesting the transformation of aromatic hydrocarbons to the liquid phase in response to biodegradation. The approach adopted in this study suggests a low diversity of the high-density bacterial population colonizing an aged oil-contaminated soil and may prove useful in selecting bacterial isolates for bioremediation studies and biotechnological applications such as biosurfactant production. Bioremediation (dpeaa)DE-He213 Bioemulsifier (dpeaa)DE-He213 Lipase (dpeaa)DE-He213 Soil microbiology (dpeaa)DE-He213 Marcelino, Paulo Ricardo Franco aut Barbosa, Aneli de Melo aut Rodrigues, Elisete Pains aut Rezende, Maria Inês aut Oliveira, André Luiz Martinez de aut Enthalten in Annals of microbiology Berlin : Springer, 1998 62(2012), 4 vom: 22. Feb., Seite 1681-1690 (DE-627)385615434 (DE-600)2143009-3 1869-2044 nnns volume:62 year:2012 number:4 day:22 month:02 pages:1681-1690 https://dx.doi.org/10.1007/s13213-012-0425-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_22 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_63 GBV_ILN_70 GBV_ILN_95 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_187 GBV_ILN_285 GBV_ILN_370 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4313 GBV_ILN_4328 GBV_ILN_4333 AR 62 2012 4 22 02 1681-1690
allfields_unstemmed	10.1007/s13213-012-0425-8 doi (DE-627)SPR030879035 (SPR)s13213-012-0425-8-e DE-627 ger DE-627 rakwb eng Granzotto, Giovana verfasserin aut Culturable bacterial pool from aged petroleum-contaminated soil: identification of oil-eating Bacillus strains 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag and the University of Milan 2012 Abstract Information gleaned from soil microbiota may provide access to new economically important species. Here, we describe the isolation, identification, and genetic diversity of high-density bacterial populations isolated from aged oil-contaminated soil. Twenty different morphotypes were identified in populations present at densities of up to $ 10^{7} $ cells $ g^{−1} $ soil, encompassing seven bacterial genera based on 16S rRNA sequencing. Six isolates of the genus Bacillus were identified, three of which appear to consume oil. The genetic clusters defined by the DNA fingerprinting analysis suggest that there is a close relationship between these oil-eating Bacillus species. Isolates able to grow using crude oil as a carbon source were biochemically characterized and found to exhibit high lipolytic activity in liquid medium and to produce alkaline-stable biosurfactants. Fluorescence spectroscopy analysis of the cell-free extract from the oil-eating Bacillus sp. strain MO.04b showed an increase in the relative fluorescence intensity of low-molecular-mass aromatics concomitantly with an increase in the protein content, suggesting the transformation of aromatic hydrocarbons to the liquid phase in response to biodegradation. The approach adopted in this study suggests a low diversity of the high-density bacterial population colonizing an aged oil-contaminated soil and may prove useful in selecting bacterial isolates for bioremediation studies and biotechnological applications such as biosurfactant production. Bioremediation (dpeaa)DE-He213 Bioemulsifier (dpeaa)DE-He213 Lipase (dpeaa)DE-He213 Soil microbiology (dpeaa)DE-He213 Marcelino, Paulo Ricardo Franco aut Barbosa, Aneli de Melo aut Rodrigues, Elisete Pains aut Rezende, Maria Inês aut Oliveira, André Luiz Martinez de aut Enthalten in Annals of microbiology Berlin : Springer, 1998 62(2012), 4 vom: 22. Feb., Seite 1681-1690 (DE-627)385615434 (DE-600)2143009-3 1869-2044 nnns volume:62 year:2012 number:4 day:22 month:02 pages:1681-1690 https://dx.doi.org/10.1007/s13213-012-0425-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_22 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_63 GBV_ILN_70 GBV_ILN_95 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_187 GBV_ILN_285 GBV_ILN_370 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4313 GBV_ILN_4328 GBV_ILN_4333 AR 62 2012 4 22 02 1681-1690
allfieldsGer	10.1007/s13213-012-0425-8 doi (DE-627)SPR030879035 (SPR)s13213-012-0425-8-e DE-627 ger DE-627 rakwb eng Granzotto, Giovana verfasserin aut Culturable bacterial pool from aged petroleum-contaminated soil: identification of oil-eating Bacillus strains 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag and the University of Milan 2012 Abstract Information gleaned from soil microbiota may provide access to new economically important species. Here, we describe the isolation, identification, and genetic diversity of high-density bacterial populations isolated from aged oil-contaminated soil. Twenty different morphotypes were identified in populations present at densities of up to $ 10^{7} $ cells $ g^{−1} $ soil, encompassing seven bacterial genera based on 16S rRNA sequencing. Six isolates of the genus Bacillus were identified, three of which appear to consume oil. The genetic clusters defined by the DNA fingerprinting analysis suggest that there is a close relationship between these oil-eating Bacillus species. Isolates able to grow using crude oil as a carbon source were biochemically characterized and found to exhibit high lipolytic activity in liquid medium and to produce alkaline-stable biosurfactants. Fluorescence spectroscopy analysis of the cell-free extract from the oil-eating Bacillus sp. strain MO.04b showed an increase in the relative fluorescence intensity of low-molecular-mass aromatics concomitantly with an increase in the protein content, suggesting the transformation of aromatic hydrocarbons to the liquid phase in response to biodegradation. The approach adopted in this study suggests a low diversity of the high-density bacterial population colonizing an aged oil-contaminated soil and may prove useful in selecting bacterial isolates for bioremediation studies and biotechnological applications such as biosurfactant production. Bioremediation (dpeaa)DE-He213 Bioemulsifier (dpeaa)DE-He213 Lipase (dpeaa)DE-He213 Soil microbiology (dpeaa)DE-He213 Marcelino, Paulo Ricardo Franco aut Barbosa, Aneli de Melo aut Rodrigues, Elisete Pains aut Rezende, Maria Inês aut Oliveira, André Luiz Martinez de aut Enthalten in Annals of microbiology Berlin : Springer, 1998 62(2012), 4 vom: 22. Feb., Seite 1681-1690 (DE-627)385615434 (DE-600)2143009-3 1869-2044 nnns volume:62 year:2012 number:4 day:22 month:02 pages:1681-1690 https://dx.doi.org/10.1007/s13213-012-0425-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_22 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_63 GBV_ILN_70 GBV_ILN_95 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_187 GBV_ILN_285 GBV_ILN_370 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4313 GBV_ILN_4328 GBV_ILN_4333 AR 62 2012 4 22 02 1681-1690
allfieldsSound	10.1007/s13213-012-0425-8 doi (DE-627)SPR030879035 (SPR)s13213-012-0425-8-e DE-627 ger DE-627 rakwb eng Granzotto, Giovana verfasserin aut Culturable bacterial pool from aged petroleum-contaminated soil: identification of oil-eating Bacillus strains 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag and the University of Milan 2012 Abstract Information gleaned from soil microbiota may provide access to new economically important species. Here, we describe the isolation, identification, and genetic diversity of high-density bacterial populations isolated from aged oil-contaminated soil. Twenty different morphotypes were identified in populations present at densities of up to $ 10^{7} $ cells $ g^{−1} $ soil, encompassing seven bacterial genera based on 16S rRNA sequencing. Six isolates of the genus Bacillus were identified, three of which appear to consume oil. The genetic clusters defined by the DNA fingerprinting analysis suggest that there is a close relationship between these oil-eating Bacillus species. Isolates able to grow using crude oil as a carbon source were biochemically characterized and found to exhibit high lipolytic activity in liquid medium and to produce alkaline-stable biosurfactants. Fluorescence spectroscopy analysis of the cell-free extract from the oil-eating Bacillus sp. strain MO.04b showed an increase in the relative fluorescence intensity of low-molecular-mass aromatics concomitantly with an increase in the protein content, suggesting the transformation of aromatic hydrocarbons to the liquid phase in response to biodegradation. The approach adopted in this study suggests a low diversity of the high-density bacterial population colonizing an aged oil-contaminated soil and may prove useful in selecting bacterial isolates for bioremediation studies and biotechnological applications such as biosurfactant production. Bioremediation (dpeaa)DE-He213 Bioemulsifier (dpeaa)DE-He213 Lipase (dpeaa)DE-He213 Soil microbiology (dpeaa)DE-He213 Marcelino, Paulo Ricardo Franco aut Barbosa, Aneli de Melo aut Rodrigues, Elisete Pains aut Rezende, Maria Inês aut Oliveira, André Luiz Martinez de aut Enthalten in Annals of microbiology Berlin : Springer, 1998 62(2012), 4 vom: 22. Feb., Seite 1681-1690 (DE-627)385615434 (DE-600)2143009-3 1869-2044 nnns volume:62 year:2012 number:4 day:22 month:02 pages:1681-1690 https://dx.doi.org/10.1007/s13213-012-0425-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_22 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_63 GBV_ILN_70 GBV_ILN_95 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_187 GBV_ILN_285 GBV_ILN_370 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4313 GBV_ILN_4328 GBV_ILN_4333 AR 62 2012 4 22 02 1681-1690
language	English
source	Enthalten in Annals of microbiology 62(2012), 4 vom: 22. Feb., Seite 1681-1690 volume:62 year:2012 number:4 day:22 month:02 pages:1681-1690
sourceStr	Enthalten in Annals of microbiology 62(2012), 4 vom: 22. Feb., Seite 1681-1690 volume:62 year:2012 number:4 day:22 month:02 pages:1681-1690
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Bioremediation Bioemulsifier Lipase Soil microbiology
isfreeaccess_bool	false
container_title	Annals of microbiology
authorswithroles_txt_mv	Granzotto, Giovana @@aut@@ Marcelino, Paulo Ricardo Franco @@aut@@ Barbosa, Aneli de Melo @@aut@@ Rodrigues, Elisete Pains @@aut@@ Rezende, Maria Inês @@aut@@ Oliveira, André Luiz Martinez de @@aut@@
publishDateDaySort_date	2012-02-22T00:00:00Z
hierarchy_top_id	385615434
id	SPR030879035
language_de	englisch
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author	Granzotto, Giovana
spellingShingle	Granzotto, Giovana misc Bioremediation misc Bioemulsifier misc Lipase misc Soil microbiology Culturable bacterial pool from aged petroleum-contaminated soil: identification of oil-eating Bacillus strains
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topic_title	Culturable bacterial pool from aged petroleum-contaminated soil: identification of oil-eating Bacillus strains Bioremediation (dpeaa)DE-He213 Bioemulsifier (dpeaa)DE-He213 Lipase (dpeaa)DE-He213 Soil microbiology (dpeaa)DE-He213
topic	misc Bioremediation misc Bioemulsifier misc Lipase misc Soil microbiology
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title	Culturable bacterial pool from aged petroleum-contaminated soil: identification of oil-eating Bacillus strains
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title_full	Culturable bacterial pool from aged petroleum-contaminated soil: identification of oil-eating Bacillus strains
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author_browse	Granzotto, Giovana Marcelino, Paulo Ricardo Franco Barbosa, Aneli de Melo Rodrigues, Elisete Pains Rezende, Maria Inês Oliveira, André Luiz Martinez de
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title_sort	culturable bacterial pool from aged petroleum-contaminated soil: identification of oil-eating bacillus strains
title_auth	Culturable bacterial pool from aged petroleum-contaminated soil: identification of oil-eating Bacillus strains
abstract	Abstract Information gleaned from soil microbiota may provide access to new economically important species. Here, we describe the isolation, identification, and genetic diversity of high-density bacterial populations isolated from aged oil-contaminated soil. Twenty different morphotypes were identified in populations present at densities of up to $ 10^{7} $ cells $ g^{−1} $ soil, encompassing seven bacterial genera based on 16S rRNA sequencing. Six isolates of the genus Bacillus were identified, three of which appear to consume oil. The genetic clusters defined by the DNA fingerprinting analysis suggest that there is a close relationship between these oil-eating Bacillus species. Isolates able to grow using crude oil as a carbon source were biochemically characterized and found to exhibit high lipolytic activity in liquid medium and to produce alkaline-stable biosurfactants. Fluorescence spectroscopy analysis of the cell-free extract from the oil-eating Bacillus sp. strain MO.04b showed an increase in the relative fluorescence intensity of low-molecular-mass aromatics concomitantly with an increase in the protein content, suggesting the transformation of aromatic hydrocarbons to the liquid phase in response to biodegradation. The approach adopted in this study suggests a low diversity of the high-density bacterial population colonizing an aged oil-contaminated soil and may prove useful in selecting bacterial isolates for bioremediation studies and biotechnological applications such as biosurfactant production. © Springer-Verlag and the University of Milan 2012
abstractGer	Abstract Information gleaned from soil microbiota may provide access to new economically important species. Here, we describe the isolation, identification, and genetic diversity of high-density bacterial populations isolated from aged oil-contaminated soil. Twenty different morphotypes were identified in populations present at densities of up to $ 10^{7} $ cells $ g^{−1} $ soil, encompassing seven bacterial genera based on 16S rRNA sequencing. Six isolates of the genus Bacillus were identified, three of which appear to consume oil. The genetic clusters defined by the DNA fingerprinting analysis suggest that there is a close relationship between these oil-eating Bacillus species. Isolates able to grow using crude oil as a carbon source were biochemically characterized and found to exhibit high lipolytic activity in liquid medium and to produce alkaline-stable biosurfactants. Fluorescence spectroscopy analysis of the cell-free extract from the oil-eating Bacillus sp. strain MO.04b showed an increase in the relative fluorescence intensity of low-molecular-mass aromatics concomitantly with an increase in the protein content, suggesting the transformation of aromatic hydrocarbons to the liquid phase in response to biodegradation. The approach adopted in this study suggests a low diversity of the high-density bacterial population colonizing an aged oil-contaminated soil and may prove useful in selecting bacterial isolates for bioremediation studies and biotechnological applications such as biosurfactant production. © Springer-Verlag and the University of Milan 2012
abstract_unstemmed	Abstract Information gleaned from soil microbiota may provide access to new economically important species. Here, we describe the isolation, identification, and genetic diversity of high-density bacterial populations isolated from aged oil-contaminated soil. Twenty different morphotypes were identified in populations present at densities of up to $ 10^{7} $ cells $ g^{−1} $ soil, encompassing seven bacterial genera based on 16S rRNA sequencing. Six isolates of the genus Bacillus were identified, three of which appear to consume oil. The genetic clusters defined by the DNA fingerprinting analysis suggest that there is a close relationship between these oil-eating Bacillus species. Isolates able to grow using crude oil as a carbon source were biochemically characterized and found to exhibit high lipolytic activity in liquid medium and to produce alkaline-stable biosurfactants. Fluorescence spectroscopy analysis of the cell-free extract from the oil-eating Bacillus sp. strain MO.04b showed an increase in the relative fluorescence intensity of low-molecular-mass aromatics concomitantly with an increase in the protein content, suggesting the transformation of aromatic hydrocarbons to the liquid phase in response to biodegradation. The approach adopted in this study suggests a low diversity of the high-density bacterial population colonizing an aged oil-contaminated soil and may prove useful in selecting bacterial isolates for bioremediation studies and biotechnological applications such as biosurfactant production. © Springer-Verlag and the University of Milan 2012
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title_short	Culturable bacterial pool from aged petroleum-contaminated soil: identification of oil-eating Bacillus strains
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Culturable bacterial pool from aged petroleum-contaminated soil: identification of oil-eating Bacillus strains

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Zugang & Verfügbarkeit

Vorhandene Bände

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