Functional characterization of two alkane hydroxylases in a versatile Pseudomonas aeruginosa strain NY3
Abstract Pseudomonas aeruginosa strain NY3 has an extraordinary capacity to utilize a wide range of substrates, including n–alkanes of lengths $ C_{5} $ to $ C_{34} $, aromatic compounds, phenols, diesel and crude oil, and it can produce a variety of small bioactive molecules, including rhamnolipids...
Ausführliche Beschreibung
Autor*in: |
Wang, Yan [verfasserIn] |
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E-Artikel |
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Sprache: |
Englisch |
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2017 |
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Anmerkung: |
© Springer-Verlag Berlin Heidelberg and the University of Milan 2017 |
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Übergeordnetes Werk: |
Enthalten in: Annals of microbiology - Berlin : Springer, 1998, 67(2017), 7 vom: 08. Juni, Seite 459-468 |
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Übergeordnetes Werk: |
volume:67 ; year:2017 ; number:7 ; day:08 ; month:06 ; pages:459-468 |
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DOI / URN: |
10.1007/s13213-017-1271-5 |
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Katalog-ID: |
SPR030888581 |
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520 | |a Abstract Pseudomonas aeruginosa strain NY3 has an extraordinary capacity to utilize a wide range of substrates, including n–alkanes of lengths $ C_{5} $ to $ C_{34} $, aromatic compounds, phenols, diesel and crude oil, and it can produce a variety of small bioactive molecules, including rhamnolipids, which can enhance its metabolic capacity for hydrophobic organic pollutants. This capacity makes NY3 a good candidate for use in environmental pollution remediation. Alkane hydroxylases catalyze both the initial and rate-limiting step of the terminal oxidation of n–alkanes. To better understand the genetic mechanisms by which P. aeruginosa NY3 degrades such a wide range of n–alkanes, two putative coding genes of alkane hydroxylases were functionally characterized using a gene-knockout approach with three different degradation systems. The single n–alkane test indicated that the hydroxylase AlkB2 acted in the early growth phase and played a major role in the utilization of $ C_{12} $–$ C_{18} $. However, a double mutant showed a trend towards recovery when $ C_{20} $–$ C_{24} $ were used as sole carbon source. This suggests that there are other enzymes capable of utilizing n–alkanes longer than $ C_{20} $. Tests of both artificial n–alkanes mixture and crude oil-containing waste water showed similar results, suggesting that both AlkB1 and AlkB2 are involved in n–alkane degradation, and, moreover, that AlkB2 plays a major role. Finally, given the wider functional range of both AlkBs in the mixture of n–alkanes compared to that of single n–alkanes, these results hint at co-metabolism. | ||
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10.1007/s13213-017-1271-5 doi (DE-627)SPR030888581 (SPR)s13213-017-1271-5-e DE-627 ger DE-627 rakwb eng Wang, Yan verfasserin aut Functional characterization of two alkane hydroxylases in a versatile Pseudomonas aeruginosa strain NY3 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag Berlin Heidelberg and the University of Milan 2017 Abstract Pseudomonas aeruginosa strain NY3 has an extraordinary capacity to utilize a wide range of substrates, including n–alkanes of lengths $ C_{5} $ to $ C_{34} $, aromatic compounds, phenols, diesel and crude oil, and it can produce a variety of small bioactive molecules, including rhamnolipids, which can enhance its metabolic capacity for hydrophobic organic pollutants. This capacity makes NY3 a good candidate for use in environmental pollution remediation. Alkane hydroxylases catalyze both the initial and rate-limiting step of the terminal oxidation of n–alkanes. To better understand the genetic mechanisms by which P. aeruginosa NY3 degrades such a wide range of n–alkanes, two putative coding genes of alkane hydroxylases were functionally characterized using a gene-knockout approach with three different degradation systems. The single n–alkane test indicated that the hydroxylase AlkB2 acted in the early growth phase and played a major role in the utilization of $ C_{12} $–$ C_{18} $. However, a double mutant showed a trend towards recovery when $ C_{20} $–$ C_{24} $ were used as sole carbon source. This suggests that there are other enzymes capable of utilizing n–alkanes longer than $ C_{20} $. Tests of both artificial n–alkanes mixture and crude oil-containing waste water showed similar results, suggesting that both AlkB1 and AlkB2 are involved in n–alkane degradation, and, moreover, that AlkB2 plays a major role. Finally, given the wider functional range of both AlkBs in the mixture of n–alkanes compared to that of single n–alkanes, these results hint at co-metabolism. Alkane hydroxylase (dpeaa)DE-He213 –alkanes (dpeaa)DE-He213 Crude oil-containing waste water (dpeaa)DE-He213 Degradation efficiency (dpeaa)DE-He213 Nie, Maiqian aut Wan, Yi aut Tian, Xiaoting aut Nie, Hongyun aut Zi, Jing aut Ma, Xia aut Enthalten in Annals of microbiology Berlin : Springer, 1998 67(2017), 7 vom: 08. Juni, Seite 459-468 (DE-627)385615434 (DE-600)2143009-3 1869-2044 nnns volume:67 year:2017 number:7 day:08 month:06 pages:459-468 https://dx.doi.org/10.1007/s13213-017-1271-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_22 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_63 GBV_ILN_95 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_187 GBV_ILN_285 GBV_ILN_370 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4313 GBV_ILN_4328 GBV_ILN_4333 AR 67 2017 7 08 06 459-468 |
spelling |
10.1007/s13213-017-1271-5 doi (DE-627)SPR030888581 (SPR)s13213-017-1271-5-e DE-627 ger DE-627 rakwb eng Wang, Yan verfasserin aut Functional characterization of two alkane hydroxylases in a versatile Pseudomonas aeruginosa strain NY3 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag Berlin Heidelberg and the University of Milan 2017 Abstract Pseudomonas aeruginosa strain NY3 has an extraordinary capacity to utilize a wide range of substrates, including n–alkanes of lengths $ C_{5} $ to $ C_{34} $, aromatic compounds, phenols, diesel and crude oil, and it can produce a variety of small bioactive molecules, including rhamnolipids, which can enhance its metabolic capacity for hydrophobic organic pollutants. This capacity makes NY3 a good candidate for use in environmental pollution remediation. Alkane hydroxylases catalyze both the initial and rate-limiting step of the terminal oxidation of n–alkanes. To better understand the genetic mechanisms by which P. aeruginosa NY3 degrades such a wide range of n–alkanes, two putative coding genes of alkane hydroxylases were functionally characterized using a gene-knockout approach with three different degradation systems. The single n–alkane test indicated that the hydroxylase AlkB2 acted in the early growth phase and played a major role in the utilization of $ C_{12} $–$ C_{18} $. However, a double mutant showed a trend towards recovery when $ C_{20} $–$ C_{24} $ were used as sole carbon source. This suggests that there are other enzymes capable of utilizing n–alkanes longer than $ C_{20} $. Tests of both artificial n–alkanes mixture and crude oil-containing waste water showed similar results, suggesting that both AlkB1 and AlkB2 are involved in n–alkane degradation, and, moreover, that AlkB2 plays a major role. Finally, given the wider functional range of both AlkBs in the mixture of n–alkanes compared to that of single n–alkanes, these results hint at co-metabolism. Alkane hydroxylase (dpeaa)DE-He213 –alkanes (dpeaa)DE-He213 Crude oil-containing waste water (dpeaa)DE-He213 Degradation efficiency (dpeaa)DE-He213 Nie, Maiqian aut Wan, Yi aut Tian, Xiaoting aut Nie, Hongyun aut Zi, Jing aut Ma, Xia aut Enthalten in Annals of microbiology Berlin : Springer, 1998 67(2017), 7 vom: 08. Juni, Seite 459-468 (DE-627)385615434 (DE-600)2143009-3 1869-2044 nnns volume:67 year:2017 number:7 day:08 month:06 pages:459-468 https://dx.doi.org/10.1007/s13213-017-1271-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_22 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_63 GBV_ILN_95 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_187 GBV_ILN_285 GBV_ILN_370 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4313 GBV_ILN_4328 GBV_ILN_4333 AR 67 2017 7 08 06 459-468 |
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10.1007/s13213-017-1271-5 doi (DE-627)SPR030888581 (SPR)s13213-017-1271-5-e DE-627 ger DE-627 rakwb eng Wang, Yan verfasserin aut Functional characterization of two alkane hydroxylases in a versatile Pseudomonas aeruginosa strain NY3 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag Berlin Heidelberg and the University of Milan 2017 Abstract Pseudomonas aeruginosa strain NY3 has an extraordinary capacity to utilize a wide range of substrates, including n–alkanes of lengths $ C_{5} $ to $ C_{34} $, aromatic compounds, phenols, diesel and crude oil, and it can produce a variety of small bioactive molecules, including rhamnolipids, which can enhance its metabolic capacity for hydrophobic organic pollutants. This capacity makes NY3 a good candidate for use in environmental pollution remediation. Alkane hydroxylases catalyze both the initial and rate-limiting step of the terminal oxidation of n–alkanes. To better understand the genetic mechanisms by which P. aeruginosa NY3 degrades such a wide range of n–alkanes, two putative coding genes of alkane hydroxylases were functionally characterized using a gene-knockout approach with three different degradation systems. The single n–alkane test indicated that the hydroxylase AlkB2 acted in the early growth phase and played a major role in the utilization of $ C_{12} $–$ C_{18} $. However, a double mutant showed a trend towards recovery when $ C_{20} $–$ C_{24} $ were used as sole carbon source. This suggests that there are other enzymes capable of utilizing n–alkanes longer than $ C_{20} $. Tests of both artificial n–alkanes mixture and crude oil-containing waste water showed similar results, suggesting that both AlkB1 and AlkB2 are involved in n–alkane degradation, and, moreover, that AlkB2 plays a major role. Finally, given the wider functional range of both AlkBs in the mixture of n–alkanes compared to that of single n–alkanes, these results hint at co-metabolism. Alkane hydroxylase (dpeaa)DE-He213 –alkanes (dpeaa)DE-He213 Crude oil-containing waste water (dpeaa)DE-He213 Degradation efficiency (dpeaa)DE-He213 Nie, Maiqian aut Wan, Yi aut Tian, Xiaoting aut Nie, Hongyun aut Zi, Jing aut Ma, Xia aut Enthalten in Annals of microbiology Berlin : Springer, 1998 67(2017), 7 vom: 08. Juni, Seite 459-468 (DE-627)385615434 (DE-600)2143009-3 1869-2044 nnns volume:67 year:2017 number:7 day:08 month:06 pages:459-468 https://dx.doi.org/10.1007/s13213-017-1271-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_22 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_63 GBV_ILN_95 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_187 GBV_ILN_285 GBV_ILN_370 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4313 GBV_ILN_4328 GBV_ILN_4333 AR 67 2017 7 08 06 459-468 |
allfieldsGer |
10.1007/s13213-017-1271-5 doi (DE-627)SPR030888581 (SPR)s13213-017-1271-5-e DE-627 ger DE-627 rakwb eng Wang, Yan verfasserin aut Functional characterization of two alkane hydroxylases in a versatile Pseudomonas aeruginosa strain NY3 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag Berlin Heidelberg and the University of Milan 2017 Abstract Pseudomonas aeruginosa strain NY3 has an extraordinary capacity to utilize a wide range of substrates, including n–alkanes of lengths $ C_{5} $ to $ C_{34} $, aromatic compounds, phenols, diesel and crude oil, and it can produce a variety of small bioactive molecules, including rhamnolipids, which can enhance its metabolic capacity for hydrophobic organic pollutants. This capacity makes NY3 a good candidate for use in environmental pollution remediation. Alkane hydroxylases catalyze both the initial and rate-limiting step of the terminal oxidation of n–alkanes. To better understand the genetic mechanisms by which P. aeruginosa NY3 degrades such a wide range of n–alkanes, two putative coding genes of alkane hydroxylases were functionally characterized using a gene-knockout approach with three different degradation systems. The single n–alkane test indicated that the hydroxylase AlkB2 acted in the early growth phase and played a major role in the utilization of $ C_{12} $–$ C_{18} $. However, a double mutant showed a trend towards recovery when $ C_{20} $–$ C_{24} $ were used as sole carbon source. This suggests that there are other enzymes capable of utilizing n–alkanes longer than $ C_{20} $. Tests of both artificial n–alkanes mixture and crude oil-containing waste water showed similar results, suggesting that both AlkB1 and AlkB2 are involved in n–alkane degradation, and, moreover, that AlkB2 plays a major role. Finally, given the wider functional range of both AlkBs in the mixture of n–alkanes compared to that of single n–alkanes, these results hint at co-metabolism. Alkane hydroxylase (dpeaa)DE-He213 –alkanes (dpeaa)DE-He213 Crude oil-containing waste water (dpeaa)DE-He213 Degradation efficiency (dpeaa)DE-He213 Nie, Maiqian aut Wan, Yi aut Tian, Xiaoting aut Nie, Hongyun aut Zi, Jing aut Ma, Xia aut Enthalten in Annals of microbiology Berlin : Springer, 1998 67(2017), 7 vom: 08. Juni, Seite 459-468 (DE-627)385615434 (DE-600)2143009-3 1869-2044 nnns volume:67 year:2017 number:7 day:08 month:06 pages:459-468 https://dx.doi.org/10.1007/s13213-017-1271-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_22 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_63 GBV_ILN_95 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_187 GBV_ILN_285 GBV_ILN_370 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4313 GBV_ILN_4328 GBV_ILN_4333 AR 67 2017 7 08 06 459-468 |
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10.1007/s13213-017-1271-5 doi (DE-627)SPR030888581 (SPR)s13213-017-1271-5-e DE-627 ger DE-627 rakwb eng Wang, Yan verfasserin aut Functional characterization of two alkane hydroxylases in a versatile Pseudomonas aeruginosa strain NY3 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag Berlin Heidelberg and the University of Milan 2017 Abstract Pseudomonas aeruginosa strain NY3 has an extraordinary capacity to utilize a wide range of substrates, including n–alkanes of lengths $ C_{5} $ to $ C_{34} $, aromatic compounds, phenols, diesel and crude oil, and it can produce a variety of small bioactive molecules, including rhamnolipids, which can enhance its metabolic capacity for hydrophobic organic pollutants. This capacity makes NY3 a good candidate for use in environmental pollution remediation. Alkane hydroxylases catalyze both the initial and rate-limiting step of the terminal oxidation of n–alkanes. To better understand the genetic mechanisms by which P. aeruginosa NY3 degrades such a wide range of n–alkanes, two putative coding genes of alkane hydroxylases were functionally characterized using a gene-knockout approach with three different degradation systems. The single n–alkane test indicated that the hydroxylase AlkB2 acted in the early growth phase and played a major role in the utilization of $ C_{12} $–$ C_{18} $. However, a double mutant showed a trend towards recovery when $ C_{20} $–$ C_{24} $ were used as sole carbon source. This suggests that there are other enzymes capable of utilizing n–alkanes longer than $ C_{20} $. Tests of both artificial n–alkanes mixture and crude oil-containing waste water showed similar results, suggesting that both AlkB1 and AlkB2 are involved in n–alkane degradation, and, moreover, that AlkB2 plays a major role. Finally, given the wider functional range of both AlkBs in the mixture of n–alkanes compared to that of single n–alkanes, these results hint at co-metabolism. Alkane hydroxylase (dpeaa)DE-He213 –alkanes (dpeaa)DE-He213 Crude oil-containing waste water (dpeaa)DE-He213 Degradation efficiency (dpeaa)DE-He213 Nie, Maiqian aut Wan, Yi aut Tian, Xiaoting aut Nie, Hongyun aut Zi, Jing aut Ma, Xia aut Enthalten in Annals of microbiology Berlin : Springer, 1998 67(2017), 7 vom: 08. Juni, Seite 459-468 (DE-627)385615434 (DE-600)2143009-3 1869-2044 nnns volume:67 year:2017 number:7 day:08 month:06 pages:459-468 https://dx.doi.org/10.1007/s13213-017-1271-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_22 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_63 GBV_ILN_95 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_187 GBV_ILN_285 GBV_ILN_370 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4313 GBV_ILN_4328 GBV_ILN_4333 AR 67 2017 7 08 06 459-468 |
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title_sort |
functional characterization of two alkane hydroxylases in a versatile pseudomonas aeruginosa strain ny3 |
title_auth |
Functional characterization of two alkane hydroxylases in a versatile Pseudomonas aeruginosa strain NY3 |
abstract |
Abstract Pseudomonas aeruginosa strain NY3 has an extraordinary capacity to utilize a wide range of substrates, including n–alkanes of lengths $ C_{5} $ to $ C_{34} $, aromatic compounds, phenols, diesel and crude oil, and it can produce a variety of small bioactive molecules, including rhamnolipids, which can enhance its metabolic capacity for hydrophobic organic pollutants. This capacity makes NY3 a good candidate for use in environmental pollution remediation. Alkane hydroxylases catalyze both the initial and rate-limiting step of the terminal oxidation of n–alkanes. To better understand the genetic mechanisms by which P. aeruginosa NY3 degrades such a wide range of n–alkanes, two putative coding genes of alkane hydroxylases were functionally characterized using a gene-knockout approach with three different degradation systems. The single n–alkane test indicated that the hydroxylase AlkB2 acted in the early growth phase and played a major role in the utilization of $ C_{12} $–$ C_{18} $. However, a double mutant showed a trend towards recovery when $ C_{20} $–$ C_{24} $ were used as sole carbon source. This suggests that there are other enzymes capable of utilizing n–alkanes longer than $ C_{20} $. Tests of both artificial n–alkanes mixture and crude oil-containing waste water showed similar results, suggesting that both AlkB1 and AlkB2 are involved in n–alkane degradation, and, moreover, that AlkB2 plays a major role. Finally, given the wider functional range of both AlkBs in the mixture of n–alkanes compared to that of single n–alkanes, these results hint at co-metabolism. © Springer-Verlag Berlin Heidelberg and the University of Milan 2017 |
abstractGer |
Abstract Pseudomonas aeruginosa strain NY3 has an extraordinary capacity to utilize a wide range of substrates, including n–alkanes of lengths $ C_{5} $ to $ C_{34} $, aromatic compounds, phenols, diesel and crude oil, and it can produce a variety of small bioactive molecules, including rhamnolipids, which can enhance its metabolic capacity for hydrophobic organic pollutants. This capacity makes NY3 a good candidate for use in environmental pollution remediation. Alkane hydroxylases catalyze both the initial and rate-limiting step of the terminal oxidation of n–alkanes. To better understand the genetic mechanisms by which P. aeruginosa NY3 degrades such a wide range of n–alkanes, two putative coding genes of alkane hydroxylases were functionally characterized using a gene-knockout approach with three different degradation systems. The single n–alkane test indicated that the hydroxylase AlkB2 acted in the early growth phase and played a major role in the utilization of $ C_{12} $–$ C_{18} $. However, a double mutant showed a trend towards recovery when $ C_{20} $–$ C_{24} $ were used as sole carbon source. This suggests that there are other enzymes capable of utilizing n–alkanes longer than $ C_{20} $. Tests of both artificial n–alkanes mixture and crude oil-containing waste water showed similar results, suggesting that both AlkB1 and AlkB2 are involved in n–alkane degradation, and, moreover, that AlkB2 plays a major role. Finally, given the wider functional range of both AlkBs in the mixture of n–alkanes compared to that of single n–alkanes, these results hint at co-metabolism. © Springer-Verlag Berlin Heidelberg and the University of Milan 2017 |
abstract_unstemmed |
Abstract Pseudomonas aeruginosa strain NY3 has an extraordinary capacity to utilize a wide range of substrates, including n–alkanes of lengths $ C_{5} $ to $ C_{34} $, aromatic compounds, phenols, diesel and crude oil, and it can produce a variety of small bioactive molecules, including rhamnolipids, which can enhance its metabolic capacity for hydrophobic organic pollutants. This capacity makes NY3 a good candidate for use in environmental pollution remediation. Alkane hydroxylases catalyze both the initial and rate-limiting step of the terminal oxidation of n–alkanes. To better understand the genetic mechanisms by which P. aeruginosa NY3 degrades such a wide range of n–alkanes, two putative coding genes of alkane hydroxylases were functionally characterized using a gene-knockout approach with three different degradation systems. The single n–alkane test indicated that the hydroxylase AlkB2 acted in the early growth phase and played a major role in the utilization of $ C_{12} $–$ C_{18} $. However, a double mutant showed a trend towards recovery when $ C_{20} $–$ C_{24} $ were used as sole carbon source. This suggests that there are other enzymes capable of utilizing n–alkanes longer than $ C_{20} $. Tests of both artificial n–alkanes mixture and crude oil-containing waste water showed similar results, suggesting that both AlkB1 and AlkB2 are involved in n–alkane degradation, and, moreover, that AlkB2 plays a major role. Finally, given the wider functional range of both AlkBs in the mixture of n–alkanes compared to that of single n–alkanes, these results hint at co-metabolism. © Springer-Verlag Berlin Heidelberg and the University of Milan 2017 |
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title_short |
Functional characterization of two alkane hydroxylases in a versatile Pseudomonas aeruginosa strain NY3 |
url |
https://dx.doi.org/10.1007/s13213-017-1271-5 |
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Nie, Maiqian Wan, Yi Tian, Xiaoting Nie, Hongyun Zi, Jing Ma, Xia |
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up_date |
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