Functional characterization of two alkane hydroxylases in a versatile Pseudomonas aeruginosa strain NY3

Abstract Pseudomonas aeruginosa strain NY3 has an extraordinary capacity to utilize a wide range of substrates, including n–alkanes of lengths $ C_{5} $ to $ C_{34} $, aromatic compounds, phenols, diesel and crude oil, and it can produce a variety of small bioactive molecules, including rhamnolipids...
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Autor*in:	Wang, Yan [verfasserIn] Nie, Maiqian Wan, Yi Tian, Xiaoting Nie, Hongyun Zi, Jing Ma, Xia

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2017

Schlagwörter:	Alkane hydroxylase –alkanes Crude oil-containing waste water Degradation efficiency

Anmerkung:	© Springer-Verlag Berlin Heidelberg and the University of Milan 2017

Übergeordnetes Werk:	Enthalten in: Annals of microbiology - Berlin : Springer, 1998, 67(2017), 7 vom: 08. Juni, Seite 459-468
Übergeordnetes Werk:	volume:67 ; year:2017 ; number:7 ; day:08 ; month:06 ; pages:459-468

Links:	Volltext

DOI / URN:	10.1007/s13213-017-1271-5

Katalog-ID:	SPR030888581

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allfields	10.1007/s13213-017-1271-5 doi (DE-627)SPR030888581 (SPR)s13213-017-1271-5-e DE-627 ger DE-627 rakwb eng Wang, Yan verfasserin aut Functional characterization of two alkane hydroxylases in a versatile Pseudomonas aeruginosa strain NY3 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag Berlin Heidelberg and the University of Milan 2017 Abstract Pseudomonas aeruginosa strain NY3 has an extraordinary capacity to utilize a wide range of substrates, including n–alkanes of lengths $ C_{5} $ to $ C_{34} $, aromatic compounds, phenols, diesel and crude oil, and it can produce a variety of small bioactive molecules, including rhamnolipids, which can enhance its metabolic capacity for hydrophobic organic pollutants. This capacity makes NY3 a good candidate for use in environmental pollution remediation. Alkane hydroxylases catalyze both the initial and rate-limiting step of the terminal oxidation of n–alkanes. To better understand the genetic mechanisms by which P. aeruginosa NY3 degrades such a wide range of n–alkanes, two putative coding genes of alkane hydroxylases were functionally characterized using a gene-knockout approach with three different degradation systems. The single n–alkane test indicated that the hydroxylase AlkB2 acted in the early growth phase and played a major role in the utilization of $ C_{12} $–$ C_{18} $. However, a double mutant showed a trend towards recovery when $ C_{20} $–$ C_{24} $ were used as sole carbon source. This suggests that there are other enzymes capable of utilizing n–alkanes longer than $ C_{20} $. Tests of both artificial n–alkanes mixture and crude oil-containing waste water showed similar results, suggesting that both AlkB1 and AlkB2 are involved in n–alkane degradation, and, moreover, that AlkB2 plays a major role. Finally, given the wider functional range of both AlkBs in the mixture of n–alkanes compared to that of single n–alkanes, these results hint at co-metabolism. Alkane hydroxylase (dpeaa)DE-He213 –alkanes (dpeaa)DE-He213 Crude oil-containing waste water (dpeaa)DE-He213 Degradation efficiency (dpeaa)DE-He213 Nie, Maiqian aut Wan, Yi aut Tian, Xiaoting aut Nie, Hongyun aut Zi, Jing aut Ma, Xia aut Enthalten in Annals of microbiology Berlin : Springer, 1998 67(2017), 7 vom: 08. Juni, Seite 459-468 (DE-627)385615434 (DE-600)2143009-3 1869-2044 nnns volume:67 year:2017 number:7 day:08 month:06 pages:459-468 https://dx.doi.org/10.1007/s13213-017-1271-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_22 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_63 GBV_ILN_95 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_187 GBV_ILN_285 GBV_ILN_370 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4313 GBV_ILN_4328 GBV_ILN_4333 AR 67 2017 7 08 06 459-468
spelling	10.1007/s13213-017-1271-5 doi (DE-627)SPR030888581 (SPR)s13213-017-1271-5-e DE-627 ger DE-627 rakwb eng Wang, Yan verfasserin aut Functional characterization of two alkane hydroxylases in a versatile Pseudomonas aeruginosa strain NY3 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag Berlin Heidelberg and the University of Milan 2017 Abstract Pseudomonas aeruginosa strain NY3 has an extraordinary capacity to utilize a wide range of substrates, including n–alkanes of lengths $ C_{5} $ to $ C_{34} $, aromatic compounds, phenols, diesel and crude oil, and it can produce a variety of small bioactive molecules, including rhamnolipids, which can enhance its metabolic capacity for hydrophobic organic pollutants. This capacity makes NY3 a good candidate for use in environmental pollution remediation. Alkane hydroxylases catalyze both the initial and rate-limiting step of the terminal oxidation of n–alkanes. To better understand the genetic mechanisms by which P. aeruginosa NY3 degrades such a wide range of n–alkanes, two putative coding genes of alkane hydroxylases were functionally characterized using a gene-knockout approach with three different degradation systems. The single n–alkane test indicated that the hydroxylase AlkB2 acted in the early growth phase and played a major role in the utilization of $ C_{12} $–$ C_{18} $. However, a double mutant showed a trend towards recovery when $ C_{20} $–$ C_{24} $ were used as sole carbon source. This suggests that there are other enzymes capable of utilizing n–alkanes longer than $ C_{20} $. Tests of both artificial n–alkanes mixture and crude oil-containing waste water showed similar results, suggesting that both AlkB1 and AlkB2 are involved in n–alkane degradation, and, moreover, that AlkB2 plays a major role. Finally, given the wider functional range of both AlkBs in the mixture of n–alkanes compared to that of single n–alkanes, these results hint at co-metabolism. Alkane hydroxylase (dpeaa)DE-He213 –alkanes (dpeaa)DE-He213 Crude oil-containing waste water (dpeaa)DE-He213 Degradation efficiency (dpeaa)DE-He213 Nie, Maiqian aut Wan, Yi aut Tian, Xiaoting aut Nie, Hongyun aut Zi, Jing aut Ma, Xia aut Enthalten in Annals of microbiology Berlin : Springer, 1998 67(2017), 7 vom: 08. Juni, Seite 459-468 (DE-627)385615434 (DE-600)2143009-3 1869-2044 nnns volume:67 year:2017 number:7 day:08 month:06 pages:459-468 https://dx.doi.org/10.1007/s13213-017-1271-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_22 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_63 GBV_ILN_95 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_187 GBV_ILN_285 GBV_ILN_370 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4313 GBV_ILN_4328 GBV_ILN_4333 AR 67 2017 7 08 06 459-468
allfields_unstemmed	10.1007/s13213-017-1271-5 doi (DE-627)SPR030888581 (SPR)s13213-017-1271-5-e DE-627 ger DE-627 rakwb eng Wang, Yan verfasserin aut Functional characterization of two alkane hydroxylases in a versatile Pseudomonas aeruginosa strain NY3 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag Berlin Heidelberg and the University of Milan 2017 Abstract Pseudomonas aeruginosa strain NY3 has an extraordinary capacity to utilize a wide range of substrates, including n–alkanes of lengths $ C_{5} $ to $ C_{34} $, aromatic compounds, phenols, diesel and crude oil, and it can produce a variety of small bioactive molecules, including rhamnolipids, which can enhance its metabolic capacity for hydrophobic organic pollutants. This capacity makes NY3 a good candidate for use in environmental pollution remediation. Alkane hydroxylases catalyze both the initial and rate-limiting step of the terminal oxidation of n–alkanes. To better understand the genetic mechanisms by which P. aeruginosa NY3 degrades such a wide range of n–alkanes, two putative coding genes of alkane hydroxylases were functionally characterized using a gene-knockout approach with three different degradation systems. The single n–alkane test indicated that the hydroxylase AlkB2 acted in the early growth phase and played a major role in the utilization of $ C_{12} $–$ C_{18} $. However, a double mutant showed a trend towards recovery when $ C_{20} $–$ C_{24} $ were used as sole carbon source. This suggests that there are other enzymes capable of utilizing n–alkanes longer than $ C_{20} $. Tests of both artificial n–alkanes mixture and crude oil-containing waste water showed similar results, suggesting that both AlkB1 and AlkB2 are involved in n–alkane degradation, and, moreover, that AlkB2 plays a major role. Finally, given the wider functional range of both AlkBs in the mixture of n–alkanes compared to that of single n–alkanes, these results hint at co-metabolism. Alkane hydroxylase (dpeaa)DE-He213 –alkanes (dpeaa)DE-He213 Crude oil-containing waste water (dpeaa)DE-He213 Degradation efficiency (dpeaa)DE-He213 Nie, Maiqian aut Wan, Yi aut Tian, Xiaoting aut Nie, Hongyun aut Zi, Jing aut Ma, Xia aut Enthalten in Annals of microbiology Berlin : Springer, 1998 67(2017), 7 vom: 08. Juni, Seite 459-468 (DE-627)385615434 (DE-600)2143009-3 1869-2044 nnns volume:67 year:2017 number:7 day:08 month:06 pages:459-468 https://dx.doi.org/10.1007/s13213-017-1271-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_22 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_63 GBV_ILN_95 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_187 GBV_ILN_285 GBV_ILN_370 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4313 GBV_ILN_4328 GBV_ILN_4333 AR 67 2017 7 08 06 459-468
allfieldsGer	10.1007/s13213-017-1271-5 doi (DE-627)SPR030888581 (SPR)s13213-017-1271-5-e DE-627 ger DE-627 rakwb eng Wang, Yan verfasserin aut Functional characterization of two alkane hydroxylases in a versatile Pseudomonas aeruginosa strain NY3 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag Berlin Heidelberg and the University of Milan 2017 Abstract Pseudomonas aeruginosa strain NY3 has an extraordinary capacity to utilize a wide range of substrates, including n–alkanes of lengths $ C_{5} $ to $ C_{34} $, aromatic compounds, phenols, diesel and crude oil, and it can produce a variety of small bioactive molecules, including rhamnolipids, which can enhance its metabolic capacity for hydrophobic organic pollutants. This capacity makes NY3 a good candidate for use in environmental pollution remediation. Alkane hydroxylases catalyze both the initial and rate-limiting step of the terminal oxidation of n–alkanes. To better understand the genetic mechanisms by which P. aeruginosa NY3 degrades such a wide range of n–alkanes, two putative coding genes of alkane hydroxylases were functionally characterized using a gene-knockout approach with three different degradation systems. The single n–alkane test indicated that the hydroxylase AlkB2 acted in the early growth phase and played a major role in the utilization of $ C_{12} $–$ C_{18} $. However, a double mutant showed a trend towards recovery when $ C_{20} $–$ C_{24} $ were used as sole carbon source. This suggests that there are other enzymes capable of utilizing n–alkanes longer than $ C_{20} $. Tests of both artificial n–alkanes mixture and crude oil-containing waste water showed similar results, suggesting that both AlkB1 and AlkB2 are involved in n–alkane degradation, and, moreover, that AlkB2 plays a major role. Finally, given the wider functional range of both AlkBs in the mixture of n–alkanes compared to that of single n–alkanes, these results hint at co-metabolism. Alkane hydroxylase (dpeaa)DE-He213 –alkanes (dpeaa)DE-He213 Crude oil-containing waste water (dpeaa)DE-He213 Degradation efficiency (dpeaa)DE-He213 Nie, Maiqian aut Wan, Yi aut Tian, Xiaoting aut Nie, Hongyun aut Zi, Jing aut Ma, Xia aut Enthalten in Annals of microbiology Berlin : Springer, 1998 67(2017), 7 vom: 08. Juni, Seite 459-468 (DE-627)385615434 (DE-600)2143009-3 1869-2044 nnns volume:67 year:2017 number:7 day:08 month:06 pages:459-468 https://dx.doi.org/10.1007/s13213-017-1271-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_22 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_63 GBV_ILN_95 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_187 GBV_ILN_285 GBV_ILN_370 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4313 GBV_ILN_4328 GBV_ILN_4333 AR 67 2017 7 08 06 459-468
allfieldsSound	10.1007/s13213-017-1271-5 doi (DE-627)SPR030888581 (SPR)s13213-017-1271-5-e DE-627 ger DE-627 rakwb eng Wang, Yan verfasserin aut Functional characterization of two alkane hydroxylases in a versatile Pseudomonas aeruginosa strain NY3 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag Berlin Heidelberg and the University of Milan 2017 Abstract Pseudomonas aeruginosa strain NY3 has an extraordinary capacity to utilize a wide range of substrates, including n–alkanes of lengths $ C_{5} $ to $ C_{34} $, aromatic compounds, phenols, diesel and crude oil, and it can produce a variety of small bioactive molecules, including rhamnolipids, which can enhance its metabolic capacity for hydrophobic organic pollutants. This capacity makes NY3 a good candidate for use in environmental pollution remediation. Alkane hydroxylases catalyze both the initial and rate-limiting step of the terminal oxidation of n–alkanes. To better understand the genetic mechanisms by which P. aeruginosa NY3 degrades such a wide range of n–alkanes, two putative coding genes of alkane hydroxylases were functionally characterized using a gene-knockout approach with three different degradation systems. The single n–alkane test indicated that the hydroxylase AlkB2 acted in the early growth phase and played a major role in the utilization of $ C_{12} $–$ C_{18} $. However, a double mutant showed a trend towards recovery when $ C_{20} $–$ C_{24} $ were used as sole carbon source. This suggests that there are other enzymes capable of utilizing n–alkanes longer than $ C_{20} $. Tests of both artificial n–alkanes mixture and crude oil-containing waste water showed similar results, suggesting that both AlkB1 and AlkB2 are involved in n–alkane degradation, and, moreover, that AlkB2 plays a major role. Finally, given the wider functional range of both AlkBs in the mixture of n–alkanes compared to that of single n–alkanes, these results hint at co-metabolism. Alkane hydroxylase (dpeaa)DE-He213 –alkanes (dpeaa)DE-He213 Crude oil-containing waste water (dpeaa)DE-He213 Degradation efficiency (dpeaa)DE-He213 Nie, Maiqian aut Wan, Yi aut Tian, Xiaoting aut Nie, Hongyun aut Zi, Jing aut Ma, Xia aut Enthalten in Annals of microbiology Berlin : Springer, 1998 67(2017), 7 vom: 08. Juni, Seite 459-468 (DE-627)385615434 (DE-600)2143009-3 1869-2044 nnns volume:67 year:2017 number:7 day:08 month:06 pages:459-468 https://dx.doi.org/10.1007/s13213-017-1271-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_22 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_63 GBV_ILN_95 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_187 GBV_ILN_285 GBV_ILN_370 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4313 GBV_ILN_4328 GBV_ILN_4333 AR 67 2017 7 08 06 459-468
language	English
source	Enthalten in Annals of microbiology 67(2017), 7 vom: 08. Juni, Seite 459-468 volume:67 year:2017 number:7 day:08 month:06 pages:459-468
sourceStr	Enthalten in Annals of microbiology 67(2017), 7 vom: 08. Juni, Seite 459-468 volume:67 year:2017 number:7 day:08 month:06 pages:459-468
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Alkane hydroxylase –alkanes Crude oil-containing waste water Degradation efficiency
isfreeaccess_bool	false
container_title	Annals of microbiology
authorswithroles_txt_mv	Wang, Yan @@aut@@ Nie, Maiqian @@aut@@ Wan, Yi @@aut@@ Tian, Xiaoting @@aut@@ Nie, Hongyun @@aut@@ Zi, Jing @@aut@@ Ma, Xia @@aut@@
publishDateDaySort_date	2017-06-08T00:00:00Z
hierarchy_top_id	385615434
id	SPR030888581
language_de	englisch
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author	Wang, Yan
spellingShingle	Wang, Yan misc Alkane hydroxylase misc –alkanes misc Crude oil-containing waste water misc Degradation efficiency Functional characterization of two alkane hydroxylases in a versatile Pseudomonas aeruginosa strain NY3
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topic_title	Functional characterization of two alkane hydroxylases in a versatile Pseudomonas aeruginosa strain NY3 Alkane hydroxylase (dpeaa)DE-He213 –alkanes (dpeaa)DE-He213 Crude oil-containing waste water (dpeaa)DE-He213 Degradation efficiency (dpeaa)DE-He213
topic	misc Alkane hydroxylase misc –alkanes misc Crude oil-containing waste water misc Degradation efficiency
topic_unstemmed	misc Alkane hydroxylase misc –alkanes misc Crude oil-containing waste water misc Degradation efficiency
topic_browse	misc Alkane hydroxylase misc –alkanes misc Crude oil-containing waste water misc Degradation efficiency
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title	Functional characterization of two alkane hydroxylases in a versatile Pseudomonas aeruginosa strain NY3
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title_full	Functional characterization of two alkane hydroxylases in a versatile Pseudomonas aeruginosa strain NY3
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author_browse	Wang, Yan Nie, Maiqian Wan, Yi Tian, Xiaoting Nie, Hongyun Zi, Jing Ma, Xia
container_volume	67
format_se	Elektronische Aufsätze
author-letter	Wang, Yan
doi_str_mv	10.1007/s13213-017-1271-5
title_sort	functional characterization of two alkane hydroxylases in a versatile pseudomonas aeruginosa strain ny3
title_auth	Functional characterization of two alkane hydroxylases in a versatile Pseudomonas aeruginosa strain NY3
abstract	Abstract Pseudomonas aeruginosa strain NY3 has an extraordinary capacity to utilize a wide range of substrates, including n–alkanes of lengths $ C_{5} $ to $ C_{34} $, aromatic compounds, phenols, diesel and crude oil, and it can produce a variety of small bioactive molecules, including rhamnolipids, which can enhance its metabolic capacity for hydrophobic organic pollutants. This capacity makes NY3 a good candidate for use in environmental pollution remediation. Alkane hydroxylases catalyze both the initial and rate-limiting step of the terminal oxidation of n–alkanes. To better understand the genetic mechanisms by which P. aeruginosa NY3 degrades such a wide range of n–alkanes, two putative coding genes of alkane hydroxylases were functionally characterized using a gene-knockout approach with three different degradation systems. The single n–alkane test indicated that the hydroxylase AlkB2 acted in the early growth phase and played a major role in the utilization of $ C_{12} $–$ C_{18} $. However, a double mutant showed a trend towards recovery when $ C_{20} $–$ C_{24} $ were used as sole carbon source. This suggests that there are other enzymes capable of utilizing n–alkanes longer than $ C_{20} $. Tests of both artificial n–alkanes mixture and crude oil-containing waste water showed similar results, suggesting that both AlkB1 and AlkB2 are involved in n–alkane degradation, and, moreover, that AlkB2 plays a major role. Finally, given the wider functional range of both AlkBs in the mixture of n–alkanes compared to that of single n–alkanes, these results hint at co-metabolism. © Springer-Verlag Berlin Heidelberg and the University of Milan 2017
abstractGer	Abstract Pseudomonas aeruginosa strain NY3 has an extraordinary capacity to utilize a wide range of substrates, including n–alkanes of lengths $ C_{5} $ to $ C_{34} $, aromatic compounds, phenols, diesel and crude oil, and it can produce a variety of small bioactive molecules, including rhamnolipids, which can enhance its metabolic capacity for hydrophobic organic pollutants. This capacity makes NY3 a good candidate for use in environmental pollution remediation. Alkane hydroxylases catalyze both the initial and rate-limiting step of the terminal oxidation of n–alkanes. To better understand the genetic mechanisms by which P. aeruginosa NY3 degrades such a wide range of n–alkanes, two putative coding genes of alkane hydroxylases were functionally characterized using a gene-knockout approach with three different degradation systems. The single n–alkane test indicated that the hydroxylase AlkB2 acted in the early growth phase and played a major role in the utilization of $ C_{12} $–$ C_{18} $. However, a double mutant showed a trend towards recovery when $ C_{20} $–$ C_{24} $ were used as sole carbon source. This suggests that there are other enzymes capable of utilizing n–alkanes longer than $ C_{20} $. Tests of both artificial n–alkanes mixture and crude oil-containing waste water showed similar results, suggesting that both AlkB1 and AlkB2 are involved in n–alkane degradation, and, moreover, that AlkB2 plays a major role. Finally, given the wider functional range of both AlkBs in the mixture of n–alkanes compared to that of single n–alkanes, these results hint at co-metabolism. © Springer-Verlag Berlin Heidelberg and the University of Milan 2017
abstract_unstemmed	Abstract Pseudomonas aeruginosa strain NY3 has an extraordinary capacity to utilize a wide range of substrates, including n–alkanes of lengths $ C_{5} $ to $ C_{34} $, aromatic compounds, phenols, diesel and crude oil, and it can produce a variety of small bioactive molecules, including rhamnolipids, which can enhance its metabolic capacity for hydrophobic organic pollutants. This capacity makes NY3 a good candidate for use in environmental pollution remediation. Alkane hydroxylases catalyze both the initial and rate-limiting step of the terminal oxidation of n–alkanes. To better understand the genetic mechanisms by which P. aeruginosa NY3 degrades such a wide range of n–alkanes, two putative coding genes of alkane hydroxylases were functionally characterized using a gene-knockout approach with three different degradation systems. The single n–alkane test indicated that the hydroxylase AlkB2 acted in the early growth phase and played a major role in the utilization of $ C_{12} $–$ C_{18} $. However, a double mutant showed a trend towards recovery when $ C_{20} $–$ C_{24} $ were used as sole carbon source. This suggests that there are other enzymes capable of utilizing n–alkanes longer than $ C_{20} $. Tests of both artificial n–alkanes mixture and crude oil-containing waste water showed similar results, suggesting that both AlkB1 and AlkB2 are involved in n–alkane degradation, and, moreover, that AlkB2 plays a major role. Finally, given the wider functional range of both AlkBs in the mixture of n–alkanes compared to that of single n–alkanes, these results hint at co-metabolism. © Springer-Verlag Berlin Heidelberg and the University of Milan 2017
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title_short	Functional characterization of two alkane hydroxylases in a versatile Pseudomonas aeruginosa strain NY3
url	https://dx.doi.org/10.1007/s13213-017-1271-5
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author2	Nie, Maiqian Wan, Yi Tian, Xiaoting Nie, Hongyun Zi, Jing Ma, Xia
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Functional characterization of two alkane hydroxylases in a versatile Pseudomonas aeruginosa strain NY3

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